Biotin DBCO is a biotinylated reagent containing a dibenzocyclooctyne (DBCO) group, enabling copper-free strain-promoted alkyne–azide cycloaddition (SPAAC) with azide-functionalized biomolecules. This reagent allows for rapid, bioorthogonal biotin labeling in living systems or sensitive samples without the need for metal catalysts, facilitating downstream detection via avidin or streptavidin binding.
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