Species-On-Species

Sensitivity. Reliability. Versatility.

Precision Solutions for Species-on-Species Detection

Species-on-species detection, such as mouse-on-mouse and human-on-human immunodetection workflows, can pose significant challenges due to high background staining and cross-reactivity with endogenous immunoglobulins. Conventional detection methods often struggle to differentiate between primary antibodies and native immunoglobulins in tissue samples, leading to non-specific staining and ambiguous results.

Vector Laboratories offers specialized immunodetection kits designed to eliminate these obstacles and enable clear, specific staining of primary antibodies in challenging species-on-species applications.

Mouse-on-Mouse (M.O.M.®) Immunodetection Kit

The Vector M.O.M. (Mouse-on-Mouse) Immunodetection Kits are engineered to address the challenge of high background staining when detecting mouse antigens in mouse tissues. Vector M.O.M. Kits offer a streamlined workflow with no complex calculations or pre-binding steps, making what was once a difficult process now simple and routine. The result? Clear, crisp, and specific antigen detection in mouse tissue samples. Ideal for use in studies involving genetically engineered mice, transgenic models, knock-out models, and xenografts.
Without M.O.M.
Sos Mom Pic1
With M.O.M.
Sos Mom Pic2
Figure 1: LEFT image: Without M.O.M. Mouse intestine stained with standard anti-mouse Ig polymer system and Vector DAB Substrate. Hematoxylin counterstain. Note background IgG staining. RIGHT image: With M.O.M. Mouse intestine stained with Vector M.O.M. ImmPRESS® Kit, Vector DAB, and no primary antibody.

Available M.O.M Immunodetection Kits

BMK-2202 

M.O.M.® (Mouse on Mouse) Immunodetection Kit, Basic   

MP-2400 
M.O.M.® (Mouse on Mouse) ImmPRESS® HRP (Peroxidase) Polymer Kit  
PK-2200 
M.O.M.® (Mouse on Mouse ) Elite® Immunodetection Kit, Peroxidase
FMK-2201 
M.O.M.® (Mouse on Mouse) Immunodetection Kit, Fluorescein
MKB-2213-1
M.O.M.® (Mouse on Mouse) Blocking Reagent
MKB-2225-.1 
M.O.M.® (Mouse on Mouse) Biotinylated Anti-Mouse IgG Reagent
MPX-2402-15 

M.O.M.® (Mouse on Mouse) ImmPRESS® Polymer Reagent, Anti-Mouse IgG, Peroxidase 

Human-on-Human (H.O.H.™) Immunodetection Kit

The Vector H.O.H. (Human-on-Human) Immunodetection Kit is designed for immunohistochemical detection of human antigens in human tissue sections. By incorporating proprietary blocking technology, the H.O.H. Kit ensures precise and specific antigen detection, even in complex human tissue samples. This system is ideal for pathology research, clinical studies, and biomarker discovery, where accurate immunohistochemical analysis of human tissues is critical.
Sos Hoh Figure2 Pic1 L
Sos Hoh Figure2 Pic2 L
Figure 2: LEFT image: Serial sections of human kidney (FFPE) showing strong, specific staining (brown regions) using human anti-cytokeratin primary antibody detected with HOH-3000. RIGHT image: Negative control. Serial sections of human kidney (FFPE) showing an absence of staining (no background) with the omission of the primary antibody in the HOH-3000 assay. Note: Both sections counterstained with hematoxylin (blue nuclei).

Humanized anti-Desmin on frozen human colon

Standard IHC Detection
Sos Hoh Colon Pic1
Vector H.O.H. Detection
Sos Hoh Colon Pic2

Humanized anti-Cytokeratin on FFPE human kidney

Standard IHC Detection
Sos Hoh Kidney Pic1
Vector H.O.H. Detection
Sos Hoh Kidney Pic2

Available H.O.H Immunodetection Kits

HOH-3000 
H.O.H.® (Human on Human) Immunodetection Kit  

Why Choose Vector Laboratories?

With decades of expertise in immunodetection solutions, Vector Laboratories is a trusted leader in species-on-species detection. Our M.O.M. and H.O.H. Kits empower researchers to overcome the inherent challenges of working with mouse and human tissue samples ensuring high-quality staining results with ease. Our solutions are relied upon by scientists worldwide for their precision, reliability, and proven effectiveness in even the most demanding immunohistochemistry (IHC) applications.

Ready to improve the accuracy and clarity of your mouse-on-mouse or human-on-human immunodetection workflows? Explore our kits and experience the Vector Laboratories difference.

Technical and Educational Resources

View our resources below for more in-depth immunofluorescence and immunohistochemistry information.
Immunofluorescence Resource Guide
Streamline the immunofluorescence process with this step-by-step guide to finding the optimal reagents for your project.
Immunohistochemistry Resource Guide
Step-by-step guide for selecting the best reagents for your immunohistochemistry workflow.
IHC Multiplexing Resource Guide
Discover the steps and considerations needed for creating stunning multicolor IHC images.