Brochures
Please find below free resources that will help you to optimize your assays. Please select one or more brochures from the list in the form, enter your contact information, and submit, and we will be happy to send you these resources free of charge. Otherwise, feel free to download and browse.
The Immunohistochemistry Resource Guide will help you navigate through the many choices of detection systems, substrates, and ancillary reagents that complete your workflow.
Tissue sample preparation includes multiple steps, and many of them offer different choices – paraffin embedding versus cryopreservation, section thickness, and antigen retrieval buffer, among others. In this guide, we will walk you through key decisions and troubleshooting issues to empower you to ask the right questions and optimize your protocols.
Blocking is a key step to ensure low background and a clear staining of the target epitope. In this guide, we will walk you through practical solutions to each potential source of non-specific staining in IHC and IF applications to empower you to improve your staining results.
A valuable resource for those considering localizing two or more antigens in the same tissue section. Protocols for chromogenic detection are accompanied by schematic illustrations of the procedures.
A chart of all pair-wise combinations of substrates is included along with photographic examples. Practical tips regarding order of substrates, blocking, and proper controls are also provided.
When applying a coverslip to your specimens, selecting the appropriate mounting medium is essential to preserve the clarity, contrast, and stability of histochemical stains and precipitable enzyme substrates.
Individual kits with essential IHC workflow components in pre-diluted, ready-to-use formats,
empower end-users with more consistent results through a reduction of assay variables, avoiding the uncertainty of using mismatched reagents and ultimately saving time.
Localize mouse primary antibodies on mouse tissue using the M.O.M. Kits to produce crisp, strong, specific staining of antibody targets.
VectaMount Express is a non-aqueous clearing and mounting medium enabling the rapid mounting of cell and tissue specimens following immunohistochemistry (IHC) staining. This novel formulation is engineered to enable mounting directly following staining, saving time by eliminating the need for extensive ethanol and clearing washes prior to coverslipping.
This ImmPRESS Enzyme Polymer brochure describes ImmPRESS micropolymer technology and includes schematics to help the user choose the most appropriate format for their application.
In addition to photomicrographs that help the user visualize the color that is generally produced
by each substrate, this brochure contains information on substrate properties that are especially useful for those who wish to multiplex (or introduce multiple labels in the same tissue section): A table of substrate properties, a schematic of relative senstitivities of substrate formulations, and a table of pairwise substrate compatibility are also included.
A quick reference guide that outlines the systematic controls to help identify background sources in an immunohistochemistry protocol using ABC systems. Also troubleshoot issues of weak or no staining. Laminated.
This Application Note describes the use of ImmPRESS HRP polymer on three popular autostainers: Dako Autostainer Plus, Leica Bond Rx, and Ventana Discovery Ultra. This Application Note includes complete staining protocols for each system along with images of stained tissue sections obtained using these protocols.
Learn about optimized Human on Human Immunodetection with the comparison of workflow and staining efficiency between the hapten-tagged method and the H.O.H. Kit method using frozen tissue sections.
This guide for troubleshooting mouse primary antibodies on mouse tissue describes some common difficulties encountered in this procedure and details strategies for customizing the M.O.M. Kits to address them.
Multiplexing is a powerful technique that enables the extraction of detailed, spatially resolved information from tissue samples and provides deeper insights into intricate biological processes. Despite its widespread use, multiplexing faces challenges including cross reactivity between rounds of detection reagents and complex assay optimizations. To address this, antibody stripping processes were pioneered.
Individual kits with essential IHC workflow components in pre-diluted, ready-to-use formats,
empower end-users with more consistent results through a reduction of assay variables, avoiding the uncertainty of using mismatched reagents and ultimately saving time.
This ImmPRESS Enzyme Polymer brochure describes ImmPRESS micropolymer technology and includes schematics to help the user choose the most appropriate format for their application.
In addition to photomicrographs that help the user visualize the color that is generally produced
by each substrate, this brochure contains information on substrate properties that are especially useful for those who wish to multiplex (or introduce multiple labels in the same tissue section): A table of substrate properties, a schematic of relative senstitivities of substrate formulations, and a table of pairwise substrate compatibility are also included.
Localize mouse primary antibodies on mouse tissue using the M.O.M. Kits to produce crisp, strong, specific staining of antibody targets.
VectaMount Express is a non-aqueous clearing and mounting medium enabling the rapid mounting of cell and tissue specimens following immunohistochemistry (IHC) staining. This novel formulation is engineered to enable mounting directly following staining, saving time by eliminating the need for extensive ethanol and clearing washes prior to coverslipping.
When applying a coverslip to your specimens, selecting the appropriate mounting medium is essential to preserve the clarity, contrast, and stability of histochemical stains and precipitable enzyme substrates.
The Immunohistochemistry Resource Guide will help you navigate through the many choices of detection systems, substrates, and ancillary reagents that complete your workflow.
Tissue sample preparation includes multiple steps, and many of them offer different choices – paraffin embedding versus cryopreservation, section thickness, and antigen retrieval buffer, among others. In this guide, we will walk you through key decisions and troubleshooting issues to empower you to ask the right questions and optimize your protocols.
Blocking is a key step to ensure low background and a clear staining of the target epitope. In this guide, we will walk you through practical solutions to each potential source of non-specific staining in IHC and IF applications to empower you to improve your staining results.
A valuable resource for those considering localizing two or more antigens in the same tissue section. Protocols for chromogenic detection are accompanied by schematic illustrations of the procedures.
A chart of all pair-wise combinations of substrates is included along with photographic examples. Practical tips regarding order of substrates, blocking, and proper controls are also provided.
Learn more about a revolutionary immunohistochemical technology, Human on Human (H.O.H.™) Immunodetection, enabling you to assess therapeutic antibodies in human tissues effortlessly in this poster.
A quick reference guide that outlines the systematic controls to help identify background sources in an immunohistochemistry protocol using ABC systems. Also troubleshoot issues of weak or no staining. Laminated.
This guide for troubleshooting mouse primary antibodies on mouse tissue describes some common difficulties encountered in this procedure and details strategies for customizing the M.O.M. Kits to address them.
This Application Note describes the use of ImmPRESS HRP polymer on three popular autostainers: Dako Autostainer Plus, Leica Bond Rx, and Ventana Discovery Ultra. This Application Note includes complete staining protocols for each system along with images of stained tissue sections obtained using these protocols.
Learn about optimized Human on Human Immunodetection with the comparison of workflow and staining efficiency between the hapten-tagged method and the H.O.H. Kit method using frozen tissue sections.
Multiplexing is a powerful technique that enables the extraction of detailed, spatially resolved information from tissue samples and provides deeper insights into intricate biological processes. Despite its widespread use, multiplexing faces challenges including cross reactivity between rounds of detection reagents and complex assay optimizations. To address this, antibody stripping processes were pioneered.
Mounting, the last step in the IF workflow, is critical for tissue integrity, optical clarity, long-term storage, signal intensity, and fluorescence preservation. This IF mounting guide provides information to help researchers choose the best mounting medium for their project and offers tips to help reduce photobleaching during the image visualization process.
Whether you’re just starting off doing immunofluorescence or are a seasoned researcher, the Immunofluorescence Guide will take you through the important steps and considerations needed
for all immunofluorescence applications. This guide offers you an overview of the IF workflow, optimization tips & tricks, and advice regarding autofluorescence (how to select the best anti-fade medium to preserve your fluorescence signal for imaging and archiving).
Product specific information for VECTASHIELD Vibrance antifade mounting media is available for download in this concise brochure. Application notes, key advantages, competitor comparison data and customer testimonials are featured.
Product specific information for VECTASHIELD PLUS Antifade Mounting Media is available for download in this concise brochure. Application notes, key advantages, competitor comparison data, informative tables & charts.
For those wanting additional details on the simple protocol and mode of action of the TrueVIEW Autofluorescence Quenching Kit, this brochure contains clear, concise scematics, comparision data with other types of quenchers, and customer testimonials.
VectaFluor™ Antibody Reagents include DyLight® dye-conjugated secondary antibodies as pre-diluted, ready-to-use solutions. VectaFluor™ Duet IF Double Labeling Kits allow you to apply two colors in one step.
VECTASHIELD Mounting Media are well-established, market-leading products that complete the workflow and provide excellent signal retention for image acquisition and specimen archiving.
In this white paper, learn about the issues of autofluorescence in immunofluorescence applications and innovations which reduce autofluorescence and improve signal to noise ratios.
VECTASHIELD Antifade Mounting Media Compatibility list – based on in-house testing and data from published papers.
Multiplexing is a powerful technique that enables the extraction of detailed, spatially resolved information from tissue samples and provides deeper insights into intricate biological processes. Despite its widespread use, multiplexing faces challenges including cross reactivity between rounds of detection reagents and complex assay optimizations. To address this, antibody stripping processes were pioneered.
Vector Laboratories offers a diverse catalog of fluorescent dyes engineered to support a wide range of applications
With multiple chemistries for easy conjugation to a variety of biomolecules, Vector fluorophores meet a wide range of scientific research needs. Our portfolio includes fluorescent dyes with top-tier performance, covering the full spectral range from UV to near-infrared, and featuring multiple reactive groups for versatile labeling.
TrueView Autofluorescence Quencher Podcast
Meet the lead scientist who developed the TrueVIEW Autofluorescence Quenching Kit. This podcast, sponsored by the National Society of Histotechnology, is a candid conversation with Tim Karpishan, Ph.D. about the development of this patent-pending product and what’s next. Listen to the Podcast
Product specific information for VECTASHIELD Vibrance antifade mounting media is available for download in this concise brochure. Application notes, key advantages, competitor comparison data and customer testimonials are featured.
Product specific information for VECTASHIELD PLUS Antifade Mounting Media is available for download in this concise brochure. Application notes, key advantages, competitor comparison data, informative tables & charts.
For those wanting additional details on the simple protocol and mode of action of the TrueVIEW Autofluorescence Quenching Kit, this brochure contains clear, concise scematics, comparision data with other types of quenchers, and customer testimonials.
VECTASHIELD Mounting Media are well-established, market-leading products that complete the workflow and provide excellent signal retention for image acquisition and specimen archiving.
VectaFluor™ Antibody Reagents include DyLight® dye-conjugated secondary antibodies as pre-diluted, ready-to-use solutions. VectaFluor™ Duet IF Double Labeling Kits allow you to apply two colors in one step.
Vector Laboratories offers a diverse catalog of fluorescent dyes engineered to support a wide range of applications
Mounting, the last step in the IF workflow, is critical for tissue integrity, optical clarity, long-term storage, signal intensity, and fluorescence preservation. This IF mounting guide provides information to help researchers choose the best mounting medium for their project and offers tips to help reduce photobleaching during the image visualization process.
Whether you’re just starting off doing immunofluorescence or are a seasoned researcher, the Immunofluorescence Guide will take you through the important steps and considerations needed
for all immunofluorescence applications. This guide offers you an overview of the IF workflow, optimization tips & tricks, and advice regarding autofluorescence (how to select the best anti-fade medium to preserve your fluorescence signal for imaging and archiving).
With multiple chemistries for easy conjugation to a variety of biomolecules, Vector fluorophores meet a wide range of scientific research needs. Our portfolio includes fluorescent dyes with top-tier performance, covering the full spectral range from UV to near-infrared, and featuring multiple reactive groups for versatile labeling.
TrueView Autofluorescence Quencher Podcast
Meet the lead scientist who developed the TrueVIEW Autofluorescence Quenching Kit. This podcast, sponsored by the National Society of Histotechnology, is a candid conversation with Tim Karpishan, Ph.D. about the development of this patent-pending product and what’s next. Listen to the Podcast
VECTASHIELD Antifade Mounting Media Compatibility list – based on in-house testing and data from published papers.
In this white paper, learn about the issues of autofluorescence in immunofluorescence applications and innovations which reduce autofluorescence and improve signal to noise ratios.
Multiplexing is a powerful technique that enables the extraction of detailed, spatially resolved information from tissue samples and provides deeper insights into intricate biological processes. Despite its widespread use, multiplexing faces challenges including cross reactivity between rounds of detection reagents and complex assay optimizations. To address this, antibody stripping processes were pioneered.
Bioconjugation provides a way to create biomolecule conjugates, with the linking of two molecules to form a single hybrid. The resulting product retains the activity of each component, yet also gains a novel function that is not possible with either molecule alone. This guide provides an overview of bioconjugation, applications and uses, as well as tools for successful bioconjugation.
This guide will take you through the necessary steps to troubleshoot common problems that you may encounter in your bioconjugation process.
dPEG®, from Vector Laboratories, are “discrete polyethylene glycol” products that can be utilized in ADC linker design. While traditional PEGs are not single compounds, each dPEG product represents a single compound with a unique, specific, single molecular weight (MW) (Figure 1). Vector Laboratories manufactures dPEG using a patented process to synthesize monodisperse linkers with longer lengths (>12 EO), orthogonal branching, greater functional group customization, and superior purity.
The Click Chemistry Toolbox by Vector Labs offers a comprehensive suite of reagents for handle introduction, handle installation, reaction acceleration, detection and tagging, multifunctional linker deployment, and target isolation for downstream enrichment.
Although ready-made biotinylated proteins are often available from commercial sources, there are many instances when specialized proteins are not available in this form, requiring the researcher to biotinylate their own protein. Making your own biotinylated proteins is not without its challenges though but thankfully, ChromaLINK Biotin allows you to biotinylate proteins quickly and easily and then confirm the number of biotins incorporated so you can proceed with confidence.
Learn about quantitative and reproducible bioconjugation with the SoluLINK® technology platform, which provides novel methods to quickly, easily and quantifiably link proteins, nucleic acids and other macromolecules to enzymes, fluorophores, haptens and other biomolecules.
SoluLINK Bioconjugation Technology provides a non-reducing, gentle method for labeling antibodies. This white paper walks you through the creation of quantifiable HRP Conjugates using SoluLINK technology.
Quantitation of biotin is important for the optimization of biotinylation procedures. This white paper will walk you through how the QuanTagTM Biotin Kit, which contains proprietary, low molecular weight chemical reagents which react with biotin, yielding a visible color change that is quantifiable spectrophotometrically, is an accurate and simple way to quantify biotin as compared to other methods such as traditional avidin-based HABA assays.
This white paper walks you through the preparation of antibody-oligonucleotide conjugates using SoluLINK Bioconjugation Technology. This methodology allows for the production of multiple antibody-oligonucleotide conjugates on the benchtop without the need for chromatographic purification.
Historically, it has been easier to design ways to use oligonucleotideprotein conjugates than it has been to prepare them. Until now. This white paper walks you through the use of SoluLINK Bioconjugation Technology to easily and efficiently prepare oligonucleotide-protein conjugates.
This report describes a UV-traceable biotinylation reagent that permits non-destructive quantification of biotin incorporation by means of a simple UV scan of the labeled antibody.
NanoLINK® Streptavidin Magnetic Beads offer unparalled binding capacity, allowing scientists to optimize the efficiency of their magnetic, bead‐based assays. This white paper details how the higher binding capacity of these beads generates reduced background and a more sensitive assay.
Antibody drug conjugates (ADCs) are one of the fastest growing modalities of treatment for various cancers as well as non-oncological conditions such as autoimmune disorders, rheumatoid arthritis and infectious diseases. As the field of precision medicine evolves, the demand for more sophisticated and effective biologics has intensified.
Nascent Protein Synthesis Assay Kits
Fluorescent Probes for Cu-free Click Chemistry
Fluorogenic Azides Probes
Azide Plus – Next Generatin Azide Probes
Iso-TaG Reagents and Kits
Cleavable Click Chemistry Biotin Probes
Click Chemistry Enrichment Kits and Media
dPEG®, from Vector Laboratories, are “discrete polyethylene glycol” products that can be utilized in ADC linker design. While traditional PEGs are not single compounds, each dPEG product represents a single compound with a unique, specific, single molecular weight (MW) (Figure 1). Vector Laboratories manufactures dPEG using a patented process to synthesize monodisperse linkers with longer lengths (>12 EO), orthogonal branching, greater functional group customization, and superior purity.
The Click Chemistry Toolbox by Vector Labs offers a comprehensive suite of reagents for handle introduction, handle installation, reaction acceleration, detection and tagging, multifunctional linker deployment, and target isolation for downstream enrichment.
Bioconjugation provides a way to create biomolecule conjugates, with the linking of two molecules to form a single hybrid. The resulting product retains the activity of each component, yet also gains a novel function that is not possible with either molecule alone. This guide provides an overview of bioconjugation, applications and uses, as well as tools for successful bioconjugation.
Antibody drug conjugates (ADCs) are one of the fastest growing modalities of treatment for various cancers as well as non-oncological conditions such as autoimmune disorders, rheumatoid arthritis and infectious diseases. As the field of precision medicine evolves, the demand for more sophisticated and effective biologics has intensified.
Nascent Protein Synthesis Assay Kits
Fluorescent Probes for Cu-free Click Chemistry
Fluorogenic Azides Probes
Azide Plus – Next Generatin Azide Probes
Iso-TaG Reagents and Kits
Cleavable Click Chemistry Biotin Probes
Click Chemistry Enrichment Kits and Media
This guide will take you through the necessary steps to troubleshoot common problems that you may encounter in your bioconjugation process.
Although ready-made biotinylated proteins are often available from commercial sources, there are many instances when specialized proteins are not available in this form, requiring the researcher to biotinylate their own protein. Making your own biotinylated proteins is not without its challenges though but thankfully, ChromaLINK Biotin allows you to biotinylate proteins quickly and easily and then confirm the number of biotins incorporated so you can proceed with confidence.
Learn about quantitative and reproducible bioconjugation with the SoluLINK® technology platform, which provides novel methods to quickly, easily and quantifiably link proteins, nucleic acids and other macromolecules to enzymes, fluorophores, haptens and other biomolecules.
SoluLINK Bioconjugation Technology provides a non-reducing, gentle method for labeling antibodies. This white paper walks you through the creation of quantifiable HRP Conjugates using SoluLINK technology.
Quantitation of biotin is important for the optimization of biotinylation procedures. This white paper will walk you through how the QuanTagTM Biotin Kit, which contains proprietary, low molecular weight chemical reagents which react with biotin, yielding a visible color change that is quantifiable spectrophotometrically, is an accurate and simple way to quantify biotin as compared to other methods such as traditional avidin-based HABA assays.
This white paper walks you through the preparation of antibody-oligonucleotide conjugates using SoluLINK Bioconjugation Technology. This methodology allows for the production of multiple antibody-oligonucleotide conjugates on the benchtop without the need for chromatographic purification.
Historically, it has been easier to design ways to use oligonucleotideprotein conjugates than it has been to prepare them. Until now. This white paper walks you through the use of SoluLINK Bioconjugation Technology to easily and efficiently prepare oligonucleotide-protein conjugates.
This report describes a UV-traceable biotinylation reagent that permits non-destructive quantification of biotin incorporation by means of a simple UV scan of the labeled antibody.
NanoLINK® Streptavidin Magnetic Beads offer unparalled binding capacity, allowing scientists to optimize the efficiency of their magnetic, bead‐based assays. This white paper details how the higher binding capacity of these beads generates reduced background and a more sensitive assay.
Get to know your lectin! This infographic will walk you through what a lectin is, how they bind to glycans, and resources to help you leverage glycan binding insights in your research.
The Lectins Application and Resource Guide is a content rich educational booklet that provides
insight into how lectins are applied in biological sciences. With a focus on plant derived lectins, the guide showcases the flexibility and utilization of lectins and lectin conjugates in established lab applications.
Exploring the World of Glycobiology eBook will walk you through a detailed background and research applications to empower you to examine the role of glycobiology in your investigations.
Examining Altered Glycobiology in Cancer will walk you through a brief background and research being performed to empower you to unlock deeper insights in your cancer investigation with glycobiology.
Explore your tissue’s sweet spot and home in on what matters most with a comprehensive view of glycan expression. Glysite Scout Glycan Screening Kits, Immunofluorescence, are fully integrated kits for the detection of glycan expression in tissue sections. Our curated lectin selection enables detection of all the major glycan motifs for the evaluation of glycan distribution in a target specimen.
The Lectins Infographic will walk you through a brief background and practical applications to empower you to integrate lectins into your protocols and grow your glycobiology toolkit.
As a part of a new journey for cancer research, we adapted the GlysiteTM Scout Glycan Screening Kit, which consists of a curated panel of lectins, to detect potential glycosylation changes between normal and cancer tissues including colon, breast, pancreas and kidney. This poster demonstrates an established, standard workflow of lectin- integrated tissue immunoassays. This poster was presented at 2023 Discover BMB Conference, as well as the 2023 joint meeting of Tissue, Matrix and Pathobiology Meeting.
Multiplexed protein and glycan analysis in native cellular contexts holds great promise for uncovering the composition, interactions, and functions of distinct cell types in complex biological systems. However, current multiplexed imaging technologies often face limitations in detection sensitivity or involve technically demanding protocols. To address these issues, we have developed an ultra-sensitive, multiplexed in situ protein and glycan profiling approach that combines reiterative staining with off-the-shelf antibodies and lectins and the newly developed Cleavable Fluorescent Probe (CFP ) cleavable TSA fluorophores. The poster was presented at 2025 AACR Conference. Please see the Press Release.
This guide will take you through potential issues you may encounter while using the Glysite™ Explorer in situ PLA Glycan Detection Kit (GEK-1000) and necessary steps to troubleshoot these problems.
Learn about this fully integrated tool for the spatial detection of glycans proximal to a protein of interest in FFPE tissues, FFPE cell pellets, and fixed cells. This system integrates a curated panel of Vector’s Glysite™ Explorer Lectins with Navinci’s proprietary in situ Proximity Ligation Assay (isPLA).
Explore your tissue’s sweet spot and home in on what matters most with a comprehensive view of glycan expression. Glysite Scout Glycan Screening Kits, Immunofluorescence, are fully integrated kits for the detection of glycan expression in tissue sections. Our curated lectin selection enables detection of all the major glycan motifs for the evaluation of glycan distribution in a target specimen.
Learn about this fully integrated tool for the spatial detection of glycans proximal to a protein of interest in FFPE tissues, FFPE cell pellets, and fixed cells. This system integrates a curated panel of Vector’s Glysite™ Explorer Lectins with Navinci’s proprietary in situ Proximity Ligation Assay (isPLA).
The Lectins Application and Resource Guide is a content rich educational booklet that provides
insight into how lectins are applied in biological sciences. With a focus on plant derived lectins, the guide showcases the flexibility and utilization of lectins and lectin conjugates in established lab applications.
Exploring the World of Glycobiology eBook will walk you through a detailed background and research applications to empower you to examine the role of glycobiology in your investigations.
Examining Altered Glycobiology in Cancer will walk you through a brief background and research being performed to empower you to unlock deeper insights in your cancer investigation with glycobiology.
As a part of a new journey for cancer research, we adapted the GlysiteTM Scout Glycan Screening Kit, which consists of a curated panel of lectins, to detect potential glycosylation changes between normal and cancer tissues including colon, breast, pancreas and kidney. This poster demonstrates an established, standard workflow of lectin- integrated tissue immunoassays. This poster was presented at 2023 Discover BMB Conference, as well as the 2023 joint meeting of Tissue, Matrix and Pathobiology Meeting.
Multiplexed protein and glycan analysis in native cellular contexts holds great promise for uncovering the composition, interactions, and functions of distinct cell types in complex biological systems. However, current multiplexed imaging technologies often face limitations in detection sensitivity or involve technically demanding protocols. To address these issues, we have developed an ultra-sensitive, multiplexed in situ protein and glycan profiling approach that combines reiterative staining with off-the-shelf antibodies and lectins and the newly developed Cleavable Fluorescent Probe (CFP ) cleavable TSA fluorophores. The poster was presented at 2025 AACR Conference. Please see the Press Release.
Get to know your lectin! This infographic will walk you through what a lectin is, how they bind to glycans, and resources to help you leverage glycan binding insights in your research.
The Lectins Infographic will walk you through a brief background and practical applications to empower you to integrate lectins into your protocols and grow your glycobiology toolkit.
This guide will take you through potential issues you may encounter while using the Glysite™ Explorer in situ PLA Glycan Detection Kit (GEK-1000) and necessary steps to troubleshoot these problems.
The family of NEUROBIOTIN products offers many advantages over other neuronal labels on the market. These products offer better solubility, are more efficiently iontophoresed, remain in the cell longer, are non-toxic, and can be fixed with formalin or glutaraldehyde.
The family of NEUROBIOTIN products offers many advantages over other neuronal labels on the market. These products offer better solubility, are more efficiently iontophoresed, remain in the cell longer, are non-toxic, and can be fixed with formalin or glutaraldehyde.
