AZDye™ 532 DBCO is an azide reactive probe that can be used for imaging azide-tagged biomolecules via a copper-free “click reaction”. DBCO moiety reacts with azides to form a stable triazole and does not require Cu-catalyst or elevated temperatures. In application where the presence of copper is a concern AZDye™ 532 DBCO is an ideal alternative to copper requiring fluorescent alkynes.
AZDye™ 532 DBCO is ideally suited for the frequency-doubled Nd:YAG laser line. This probe is water-soluble and its fluorescence is pH independent over a wide pH range. The brightness and photostability of AF 532 dyes are well suited to direct imaging of low-abundance targets.
AZDye™ 532 is spectrally almost identical to many other fluorescent dyes based on sulfonated rhodamine core, including Alexa Fluor® 532 and CF®532 Dye.
| Unit Size | 1 mg, 5 mg, 25 mg |
|---|---|
| Abs/Em Maxima | 532/554 nm |
| Extinction Coefficient | 78,000 |
| Flow Cytometry Laser Line | 532 nm |
| Microscopy Laser Line | 532 nm |
| Spectrally Similar Dyes | Alexa Fluor® 532, Atto™ 532, CF® 532 dyes |
| Molecular weight | 872.92 |
| CAS | N/A |
| Solubility | Water, DMSO, DMF |
| Purity | >95% (HPLC) |
| Appearance | Orange to light red solid |
| Storage Conditions | -20°C. Desiccate |
| Shipping Conditions | Ambient temperature |
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