AZDye™ 488 NHS Ester is an amine reactive, green-emitting dye routinely used to label proteins or antibodies through primary amines (Lys), amine-modified oligonucleotides, and other amine-containing biomolecules. The labeling occurs most efficiently at pH 7-9 and forms a stable, covalent amide bond.
AZDye™ 488 is a water-soluble, highly photostable dye with a very high fluorescence quantum yield. AZDye 488 has green fluorescence that is pH independent from pH 4 to pH 10 with excitation ideally suited for the 488 nm laser line. AZDye™ 488 dye can be conjugated to a variety of antibodies, peptides, proteins, tracers, and amplification substrates which are often used for generation of stable signals in imaging and flow cytometry.
AZDye™ 488 Ester is structurally identical to Alexa Fluor® 488 NHS Ester. Its absorption/emission spectra is a perfect match to spectra of many other fluorescent dyes based on sulfonated rhodamine 110 core, including DyLight® 488, Alexa Fluor® 488, and CF® 488 Dye.
| Unit Size | 1 mg, 5 mg, 25 mg, 100 mg |
|---|---|
| Reactivity | Primary amines |
| Abs/Em Maxima | 494/517 nm |
| Extinction coefficient | 73,000 cm-1M-1 |
| Solubility | Water, DMSO, DMF |
| Spectrally similar dyes | Alexa Fluor® 488, DyLight® 488, Fluorescein, Oregon Green 488 |
| Molecular weight | 631.54 (protonated) |
| Storage Conditions | -20°C. |
| Shipping Conditions | Ambient temperature |
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