Fluorophore Conjugated

Fluorophore-conjugated avidin and biotinylated reagents are specialized tools used in protein detection and visualization, particularly in fluorescence-based techniques. These reagents combine the strong, specific interaction between biotin and avidin/streptavidin/neutravidin with the optical sensitivity of fluorescent dyes (fluorophores), enabling precise localization and quantification of target biomolecules.

Vector Laboratories offers a comprehensive range of fluorophore-conjugated avidin and biotinylated reagents for protein detection and visualization, optimized for applications such as fluorescence microscopy, flow cytometry, and molecular imaging. This product line includes fluorescein-labeled avidin derivatives like Fluorescein Avidin D, FITC, suitable for general fluorescence detection, and Fluorescein Avidin DCS, Cell Sorting Grade, which is charge-modified for reduced background in flow cytometry applications. Complementing these are a wide array of streptavidin-fluorophore conjugates designed for versatility across the fluorescence spectrum. These include Streptavidin, CY3 and CY5, ideal for red and far-red imaging, as well as conjugates labeled with DyLight® dyes—specifically DyLight 488, 549, 594, and 649—which offer high brightness and photostability for multiplexed or long-exposure imaging. Additional options like Streptavidin, Fluorescein and Streptavidin, Texas Red™ provide further flexibility for classical fluorescence detection platforms. Together, these reagents form a powerful toolkit for precise, high-sensitivity detection of biotin-labeled targets in a variety of biological and analytical systems.

These reagents are widely used in immunofluorescence (IF), fluorescence in situ hybridization (FISH), cell surface labeling, and glycan detection, providing high specificity, multiplexing capability, and minimal background. Their modular format enables flexible assay design and strong signal amplification while preserving biological integrity in fixed or live-cell systems.