Enhance Bioconjugation with High-Efficiency CuAAC Click Chemistry Reagents

Click chemistry reactions are renowned for their outstanding selectivity, efficiency, and versatility across a wide range of applications.

Among them, the Copper-Catalyzed Azide-Alkyne Cycloaddition (CuAAC) is one of the most widely used and flexible reactions. CuAAC involves a 1,3-dipolar cycloaddition that links an azide and an alkyne in the presence of a copper(I) catalyst, producing a stable 1,4-disubstituted-1,2,3-triazole. Known for its high yields and exceptional specificity, CuAAC click chemistry has become a vital tool in both biological and chemical research

Cuaac Ligation Reaction
Schematic representation of copper-catalyzed click reaction.
The azide and alkyne functional groups are largely inert to biological systems allowing them to be incorporated into proteins, nucleic acids, sugars, and other biomolecules without disrupting normal biological processes. This makes CuAAC highly effective in diverse applications delivering efficient, high-yield results even in complex environments.
Vector Laboratories offers a comprehensive portfolio of high-purity azide and terminal alkyne reagents to deliver complete solutions for your experimental needs. With high purity, consistency, and rigorous quality control, our reagents ensure reproducibility, reliability, and high performance across broad applications.

The Role of Accelerating Ligands in CuAAC:

To further enhance the efficiency and biocompatibility of CuAAC reactions Accelerating Ligands act to stabilize the copper catalyst and reduce cytotoxicity. For researchers looking to optimize CuAAC for specific applications, a variety of ligands are available to optimize reaction conditions to maximum efficiency and minimize cytotoxicity: THPTA, BTTAA, BTTES, BTTP, and TBTA.

Key Features of CuAAC Reactions:

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Selectivity:
The CuAAC reaction forms covalent bonds in richly functionalized environments allowing precise conjugations to occur.
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Solvent Versatility:
CuAAC can be performed in both aqueous and organic solvents, offering flexibility in reaction conditions based on the needs of the specific experiment or synthesis.
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Small, Inert Tags:
Azide and alkyne tags are small, inert functional groups compatible with various biomolecules and metabolic processes.
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Quantitative Yields:
CuAAC azide-alkyne reactions typically achieve quantitative or near-quantitative yields reducing waste and simplifying post-reaction purification steps.
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Versatile Options:
CuAAC reactions are broadly applicable with azide-functionalized reagents capable of conjugating to terminal alkyne-modified molecules such as proteins, peptides, nucleic acids, and small molecules.

Key Benefits of CuAAC Reactions:

Superior Selectivity:
CuAAC exhibits excellent selectivity ensuring highly specific ligation between azide- and alkyne-modified biomolecules, minimizing off-target interactions.
Higher Efficiency:
Achieving near-quantitative yields minimizes waste and reduces the need for extensive purification, streamlining workflows and processes.
Flexible Reaction Conditions:
The ability to perform CuAAC in both aqueous and organic solvents allow for usage in both biological systems or synthetic organic chemistry.

Broad Applicability of CuAAC Reactions:

  • Biomolecule Conjugation (ex. proteins, peptides, glycans, nucleic acids, lipids, nanoparticles)
  • Organic Synthesis
  • Nanotechnology and Material Science
  • Metabolic Labeling
  • Peptide and Protein Engineering
  • Tissue Engineering and Regenerative Medicine
  • Chemical Biology
  • Surface functionalization
  • Metabolomics
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to take the next step in your bioconjugate journey.