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Glysite™ Explorer in situ PLA Glycan Detection Kit
Go beyond protein expression: unlock a new dimension of glycosylation in cells and tissues
The Glysite™ Explorer in situ PLA Glycan Detection Kit is a fully integrated tool for the spatial detection of glycans proximal to a protein of interest in FFPE tissues, FFPE cell pellets, and fixed cells. This kit integrates a curated panel of Vector’s Glysite™ Explorer Lectins and detection reagents with Navinci’s proprietary in situ Proximity Ligation Assay (isPLA). Glysite™ Explorer Lectins, covering eight major glycan categories, can be selected individually by researchers to pair with their primary antibody targeting a protein of interest. This provides a simple approach to understand protein glycosylation with a spatial context.
Protein Glycosylation, Visualized
Reveal glycosylation patterns to unlock deeper biological insights.
- Investigate the glycosylation status of proteins and validate glycan-modulated targets
- Gain orthogonal data that goes beyond standard spatial protein detection
- Fast, easy, and reproducible workflow
Seamless Integration, No Special Setup
Gain insights into glycosylation without changing your workflow.
- Designed to plug directly into standard IHC and isPLA protocols
- No need for specialized equipment, additional training, or complex setup
- Enables additional glycosylation analysis using existing samples and processes
Diverse Sample Compatibility
Analyze glycosylation across a wide range of sample types.
- Works with FFPE tissues, FFPE cells, and fixed cells
- Ideal for biomarker discovery, translational research, and the study of disease biology
- Enables comparative studies across archived and fresh samples
Reliable, Validated Technology
Built for confidence and reproducibility.
- Uses validated lectins and a robust isPLA technology
- Curated components ensure specificity and consistency across experiments
- Backed by optimized protocols and quality manufacturing processes
How Glysite™ Explorer Empowers Research
Use Case: Immune Checkpoint Investigations with Harvard’s Barthel and Schatton Labs
Dr. Steven Barthel and Dr. Tobias Schatton co-direct the Program of Glyco-Immunology and Oncology (PGIO) at Brigham and Women’s Hospital, Harvard Medical School. Their teams use the Glysite™ Explorer isPLA Glycan Detection Kit to explore the proximity of glycans to immune checkpoint proteins in melanoma cells and a variety of immune cell subsets. This data will add to the growing understanding of the impact of glycosylation on the tumor microenvironment and cancer progression.
isPLA of melanoma cells using the Glysite Explorer Kit and a Glysite Explorer Lectin paired with a primary antibody against an immune checkpoint protein.
isPLA of melanoma cells using the Glysite Explorer Kit and a Glysite Explorer Lectin paired with a primary antibody against an immune checkpoint protein.
How the Glysite™ Explorer in situ PLA Glycan Detection Kit Works
Why Choose the Glysite™ Explorer in situ PLA Glycan Detection Kit?
Protein glycosylation is a vital post-translational modification that influences protein function, stability, and cellular communication. It plays a major role in health and disease, making it a key area of research in biotechnology, pharmaceuticals, and disease diagnostics.
This kit is the first of its kind and plugs into your IHC workflow for the spatial visualization of protein-glycan proximity, unlocking a new dimension of data from your samples. Built on Vector’s validated lectins and a robust isPLA technology, this kit provides an optimized protocol for evaluating the glycosylation states of proteins.
| Features | Benefits |
|---|---|
|
Novel tool for spatial visualization of proteins and glycans of your choice |
In situ detection of glycans proximal to a target protein in specimens using lectins as glycan binders. |
|
Provides a new lens to advance functional proteomics |
This tool can be used to understand protein-specific glycosylation, protein-glycan interactions, or detect glycosylation changes associated with disease states. |
|
Utilizes existing equipment and workflow |
Compatible with FFPE tissues, FFPE cell pellets, and fixed cells. Leverages routinely used lectins, primary antibodies, and isPLA workflows to provide a rapid assessment of specimens. No need for specialized equipment. |
|
Leverages the well-established isPLA technology |
The workflow is optimized and validated for performance and reproducibility. The Navinci isPLA technology is supported by a strong record of publications. |
Product Configuration and Ordering
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Resources
Learn more about how the Glysite™ Explorer Kit provides a streamlined approach to unravel protein glycosylation in any sample.
Guidebook: Glycobiology and Cancer
Learn more about how Vector reagents support investigation of complex glycans in cancer research.
Lectin Infographic
Explore how lectins serve as powerful research tools for a variety of applications.
Video Presentation
Unraveling Protein Glycosylation with the
New Glysite™ Explorer in situ PLA Glycan Detection Kit
Explore the world of glycobiology, including implications in health and disease and applications of lectins.
Explore our suite of products and resources to help researchers profile, characterize, and capture complex glycans in biological systems.
Press Release: Advancing Functional Proteomics with Navinci
This continuation of our partnership with Navinci is part of our shared mission to bring glyco-insights to researchers everywhere.
Have questions about the Glysite™ Explorer Kit and Glysite™ Explorer Lectins? Need guidance on other glycobiology research tools? Want to make an order? Consult with our experts free of cost. Fill in your information and one of our experts will get in touch with you.
