Vector® TrueVIEW® Autofluorescence Quenching Kit
Dramatically reduce autofluorescence to reveal true immunofluorescence
In tissue sections, autofluorescence is the unwanted fluorescence that can make it difficult or impossible to distinguish antigen-specific signal from non-specific background noise. The novel TrueVIEW Autofluorescence Quenching Kit specifically binds and quenches autofluorescent elements from non-lipofuscin sources, significantly enhancing signal-to-noise in most immunofluorescence assays—even in the most challenging tissues.
WITH TrueVIEW Quencher
WITHOUT Treatment
Reduction of autofluorescence in human spleen using the TrueVIEW Autofluorescence Quenching Kit. Human spleen sections (FFPE) stained using mouse anti-CD20 (red) and rabbit anti-Ki67 (green) primary antibodies detected with VectaFluor™ Duet kit (DK-8818). Note significant reduction of autofluorescence in the treated section.
Our customers have great things to say about the Vector TrueVIEW Autofluorescence Kit!
Why TrueVIEW Quencher?
- Specific reduction of autofluorescence from non-lipofuscin sources
- Easy-to-use, one-step method
- Quick 5 min incubation
- Compatible with a wide selection of fluorophores
- Compatible with standard epifluorescence and confocal laser microscopes
Comparison with other autofluorescence reducing agents
Whereas most methods for reducing tissue autofluorescence act primarily on lipofuscin granules, the TrueVIEW quencher targets fluorescence from non-lipofuscin sources, including aldehyde fixation, red blood cells, and structural elements, such as collagen and elastin. It provides a clear, unambiguous “true view” of target antigen localization, even in problematic tissues, such as kidney, spleen, and pancreas.
Comparisons with other commercial and “home brew” approaches show that Vector TrueVIEW is easier to use and more effective at reducing autofluorescence. The images below show the results of side-by-side comparisons on serial sections of formalin-fixed, paraffin embedded human pancreas visualized using a standard fluorescein (green) filter. No specific immunofluorescence staining was conducted.
No Treatment (Endogenous autofluorescence)
TrueVIEW Quencher Treated
Competitive treatments for dealing with autofluorescence
Company A
Company B
Company C
Alternative treatments for dealing with autofluorescence
Copper Sulfate Solution
Sodium Borohydride
Sudan Black B
Additional resources available from Vector Labs
Listen to the podcast
LISTEN to the podcast by Timothy Karpishin, PhD, Director of Chemistry at Vector Laboratories, Inc. describing TrueVIEW Autofluorescence Quenching Kit at the 43rd Annual NSH Symposium/Convention.
Download the white paper
DOWNLOAD the white paper to learn about the issue of autofluorescence in immunofluorescence applications along with innovations that reduce autofluorescence and improve signal to noise ratios.
Download the brochure
Learn more about the Vector TrueVIEW Autofluorescence Quenching Kit by downloading the product brochure.
The TrueVIEW Autofluorescence Kit is easy to use
Following completion of immunofluorescence staining:
Mode of Action
Product Details & Ordering Information
| Product | Catalog Number | Applications | Unit Size |
|
TrueVIEW Autofluorescence Quenching Kit |
SP-8400 | Immunofluorescence, In situ hybridization | 15 mL |
|
TrueVIEW Autofluorescence Quenching Kit with DAPI |
SP-8500 | Immunofluorescence, In situ hybridization | 15 mL |
|
VECTASHIELD® Vibrance™ Antifade Mounting Medium |
H-1700 | Immunofluorescence, In situ hybridization, Cellular Imaging | 2 mL, 10 mL |
|
VECTASHIELD Vibrance™ with DAPI Antifade Mounting Medium |
H-1800 | Immunofluorescence, In situ hybridization, Cellular Imaging | 2 mL, 10 mL |
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