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ImmPACT® DAB EqV Substrate Kit, Peroxidase (HRP) (SK-4103)



ImmPACT DAB EqV consists of equal volumes of Reagent 1 and Reagent 2, and when mixed together form a working solution. ImmPACT DAB EqV produces a dark brown reaction product and is 3 to 5 times more sensitive than the original DAB substrate.


  • Optimized for IHC/ICC/ISH
  • Heat Stable
  • Suitable for permanent mounting


Unit Size100 ml, 400 ml
ApplicationsImmunohistochemistry / Immunocytochemistry, In situ hybridization, Blotting Applications, Elispot
Detection Enzyme(s)Peroxidase
Substrate Reaction ProductPrecipitating Reaction Product
Chromogen ColorBrown
MicroscopyBrightfield, Darkfield, Electron, Spectral Imaging
Additional Substrate PropertiesHeat Resistant, Multiple Labeling
MountingAqueous (Hardening), Aqueous (Non-Hardening), Non-Aqueous (Permanent)

Technical Information

DAB (3,3′-diaminobenzidine) HRP substrate produces a dark brown reaction product and can be used for both immunohistochemical and blotting applications.The DAB chromogen is effective as a single label or as a second color for multiple antigen labeling. Because of its heat-resistance, DAB can be used in IHC/ ISH double labeling applications. With the aid of imaging systems and software, the spectral profile of DAB can be distinguished from our other proprietary enzyme substrates in applications where antigens are co-localized. Sections stained with DAB can also be viewed by darkfield and electron microscopy. Slides developed with DAB can be dehydrated, cleared, and permanently mounted. The DAB reaction product can be intensified with DAB Enhancing Solution (H-2200).

The working solution is stable for several days when stored at 4°C. This substrate can be used for both manual and automated staining methods. ImmPACT DAB EqV can be used for blot detection on nylon and PVDF membranes but is not recommended for nitrocellulose membranes.


SUBSTRATEsensitivity2 jpg Substrate.CounterstainChart jpg SubstrateCombos jpg

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