Blocking Reagents

Blocking reagents are used in protein detection techniques to prevent non-specific binding of antibodies or probes to surfaces. They work by occupying potential binding sites on membranes, plates, or tissues that might otherwise attract antibodies non-specifically. Proper blocking is essential for reducing background noise and increasing the accuracy of detection. This step is routinely used in methods such as Western Blotting, ELISA, and Immunohistochemistry (IHC).

We provide a comprehensive range of blocking reagents—including protein blocking solutions, Western Blot blocking buffers, endogenous biotin and enzyme blockers, sera, mouse Ig blocking reagents, and autofluorescence quenchers—designed to reduce non-specific binding and background in diverse assays.