Category: What’s New – VectorLabs https://staging.vectorlabs.com From linker design and synthesis, bioconjugated design and manufacturing, biomolecule labeling and functionalization, and detection system and solutions, our team is here to help you move forward, with impact. Wed, 22 Oct 2025 17:15:13 +0000 en-US hourly 1 https://wordpress.org/?v=6.8.3 https://staging.vectorlabs.com/wp-content/uploads/2024/11/V-favicon-02-100x100.png Category: What’s New – VectorLabs https://staging.vectorlabs.com 32 32 VectaPlex™ Antibody Removal Kit https://staging.vectorlabs.com/products/vectaplex/ Wed, 28 Jun 2023 19:40:14 +0000 https://staging.vectorlabs.com/?post_type=product&p=16641 Description

Your path to simplified multiplexing starts with VectaPlex™.  This kit is designed to remove antibodies and other non-covalently bound detection reagents from FFPE tissue sections in immunohistochemistry (IHC), immunofluorescence (IF) and tyramide signal amplification (TSA)  workflows.

Benefits include:

  • Removes antibodies and other non-covalently bound detection reagents from FFPE tissue sections​
  • Compatible with immunofluorescence-, fluorescent tyramide- and immunohistochemistry-based workflows​
  • Obtain more spatial biomarker information from each tissue section​
  • Stain with multiple primaries from the same species and a single secondary without cross reactivity​
  • Maintains tissue morphology through multiple cycles – even on delicate specimens like breast & skin
  • Maintains tissue antigenicity through multiple cycles​
  • Primary antibody concentrations do not need to be optimized ​based on the order of detection in multiplex workflows​
  • Easy-to-use, room temperature application​

 

The first image above is a composite image generated from individual images taken during six rounds of immunofluorescence staining using VectaPlex.  FFPE stomach; six mouse primary antibodies – CD20 (red), CD34 (yellow), Desmin (cyan), AE1/AE3 (magenta), CD3 (gray), Vimentin (green) – detected with DyLight™ 488 horse anti-mouse IgG.  Blue indicates DAPI stain.  Vector® TrueView® Autofluorescence Quencher Kit applied to suppress background fluorescence.

The second image above is a 4-plex IHC image taken of esophagus tissue stained for CD68 (brown) using ImmPACT DAB, CD20 (red) using ImmPACT Vector Red, CD34 (blue) using Vector Blue, and Desmin (grey) using ImmPACT SG.  Each round of staining was performed using normal IHC protocols with VectaPlex treatment between each round of staining.

The third image above was captured after three rounds of tyramide staining. VectaPlex was used after each round. FFPE tonsil is shown, following application of 3 mouse primary antibodies – CD3 (red), CD68 (green) and AE1/AE3 (purple) – detected using Opal 3-plex TSA Manual Detection Kit. Blue indicates DAPI stain.

Specifications

Unit Size 125 reactions /  kit
Applications Immunohistochemistry, Immunofluorescence, Tyramide Signal Amplification
Target Species Universal
Recommended Storage (15–25° C)
Format Ready-to-Use

The post VectaPlex™ Antibody Removal Kit appeared first on VectorLabs.

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Description

Your path to simplified multiplexing starts with VectaPlex™.  This kit is designed to remove antibodies and other non-covalently bound detection reagents from FFPE tissue sections in immunohistochemistry (IHC), immunofluorescence (IF) and tyramide signal amplification (TSA)  workflows.

Benefits include:

  • Removes antibodies and other non-covalently bound detection reagents from FFPE tissue sections​
  • Compatible with immunofluorescence-, fluorescent tyramide- and immunohistochemistry-based workflows​
  • Obtain more spatial biomarker information from each tissue section​
  • Stain with multiple primaries from the same species and a single secondary without cross reactivity​
  • Maintains tissue morphology through multiple cycles – even on delicate specimens like breast & skin
  • Maintains tissue antigenicity through multiple cycles​
  • Primary antibody concentrations do not need to be optimized ​based on the order of detection in multiplex workflows​
  • Easy-to-use, room temperature application​

The first image above is a composite image generated from individual images taken during six rounds of immunofluorescence staining using VectaPlex.  FFPE stomach; six mouse primary antibodies – CD20 (red), CD34 (yellow), Desmin (cyan), AE1/AE3 (magenta), CD3 (gray), Vimentin (green) – detected with DyLight™ 488 horse anti-mouse IgG.  Blue indicates DAPI stain.  Vector® TrueView® Autofluorescence Quencher Kit applied to suppress background fluorescence.

The second image above is a 4-plex IHC image taken of esophagus tissue stained for CD68 (brown) using ImmPACT DAB, CD20 (red) using ImmPACT Vector Red, CD34 (blue) using Vector Blue, and Desmin (grey) using ImmPACT SG.  Each round of staining was performed using normal IHC protocols with VectaPlex treatment between each round of staining.

The third image above was captured after three rounds of tyramide staining. VectaPlex was used after each round. FFPE tonsil is shown, following application of 3 mouse primary antibodies – CD3 (red), CD68 (green) and AE1/AE3 (purple) – detected using Opal 3-plex TSA Manual Detection Kit. Blue indicates DAPI stain.

Specifications

Unit Size125 reactions /  kit
ApplicationsImmunohistochemistry, Immunofluorescence, Tyramide Signal Amplification
Target SpeciesUniversal
Recommended Storage(15–25° C)
FormatReady-to-Use

Documents

Technical Information

This ready-to-use kit is designed to remove antibodies and other non-covalently bound detection reagents from formalin-fixed paraffin-embedded (FFPE) tissue sections in immunofluorescence or tyramide signal amplification staining workflows.

VectaPlex simplifies sample processing for manual workflows, accelerates multiplex assay optimization, preserves precious sample, and maintains tissue integrity and morphology through multiple cycles of antibody binding and removal.

 

 

VectaPlex IHC Workflow: After staining with one of the approved substrates, VectaPlex is used to remove detection reagents in preparation for another cycle of staining. The substrate is not removed or impacted by VectaPlex. A single image is collected after all cycles are complete.

IHC Workflow Web

VectaPlex IF Workflow: After IF staining and imaging the sample, VectaPlex is used to remove detection reagents in preparation for another cycle of staining. After all images have been collected, a composite image is formed from individual images.

IF Workflow web

VectaPlex TSA Workflow: After TSA staining and imaging the sample, VectaPlex is used to remove detection reagents in preparation for another cycle of staining. The tyramide sigal is not removed by VectaPlex. A single image is collected after all cycles are complete.

TSA workflow Web

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]]> Animal-Free Blocker® and Diluent, R.T.U. https://staging.vectorlabs.com/products/animal-free-block-and-diluent-afb/ Thu, 04 May 2023 04:53:03 +0000 https://staging.vectorlabs.com/?post_type=product&p=15151 Description

R.T.U. Animal Free Blocker and Diluent is a ready-to-use solution designed to be used as an antibody diluent and a blocking reagent to stabilize diluted antibody solutions and diminish background due to non-specific binding in immunohistochemical applications. This product contains no animal-derived proteins. The R.T.U. Animal Free Blocker and Diluent is especially convenient in multiplex IHC (multiple antigen labeling) applications where antibodies from several different species are used.

Specifications

Unit Size 100 ml
Applications Immunohistochemistry / Immunocytochemistry, Immunofluorescence, Blotting Applications, Elispot
Recommended Storage 2-8 °C
Recommended Usage This product is designed to be used as an antibody diluent and a blocking reagent to stabilize diluted antibody solutions and diminish background due to non-specific binding in immunohistochemical applications. This reagent contains no animal-derived proteins and can be used as an alternative to sera, BSA, casein or non-fat dry milk in situations when animal protein-based blocking solutions cause increased background or interfere with blot development. Primary and secondary antibodies can be diluted in this solution and stored at 4 ºC for up to a week.
Blocking Action Non-Specific Protein Blocking
Format Ready-to-Use

The post Animal-Free Blocker® and Diluent, R.T.U. appeared first on VectorLabs.

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Description

R.T.U. Animal Free Blocker and Diluent is a ready-to-use solution designed to be used as an antibody diluent and a blocking reagent to stabilize diluted antibody solutions and diminish background due to non-specific binding in immunohistochemical applications. This product contains no animal-derived proteins. The R.T.U. Animal Free Blocker and Diluent is especially convenient in multiplex IHC (multiple antigen labeling) applications where antibodies from several different species are used. 

Specifications

Unit Size100 ml
ApplicationsImmunohistochemistry / Immunocytochemistry, Immunofluorescence, Blotting Applications, Elispot
Recommended Storage2-8 °C
Recommended UsageThis product is designed to be used as an antibody diluent and a blocking reagent to stabilize diluted antibody solutions and diminish background due to non-specific binding in immunohistochemical applications. This reagent contains no animal-derived proteins and can be used as an alternative to sera, BSA, casein or non-fat dry milk in situations when animal protein-based blocking solutions cause increased background or interfere with blot development. Primary and secondary antibodies can be diluted in this solution and stored at 4 ºC for up to a week.
Blocking ActionNon-Specific Protein Blocking
FormatReady-to-Use

Documents

Product FAQs

Both products are derived from the same plant protein and in some applications can be used interchangeably. At a high level, one is presented as a ready-to-use (RTU) format and the other a more economical concentrate. The key difference is that the RTU format has been specifically optimized for use in IHC and IF applications. The RTU format has a neutral pH suitable for tissue and cell-based assays and has been more highly refined to avoid the presence of flocculent material that may cause interference with microscopy work. The 5x concentrated format is best suited for membrane blotting applications.

Technical Information

This plant protein derived product is a universal antibody diluent and blocking reagent intended for cell and tissue based immunohistochemistry (IHC) and immunofluorescent (IF) applications. This ready-to-use solution can be used as an alternative to normal sera, BSA, casein and non-fat dry milk in methods where these or other blocking agents have been found to be ineffective.

It is supplied without any sodium azide, and as such, can be used with both peroxidase and alkaline phosphatase antibody conjugates and all secondary detection reagents including polymer systems and avidin/biotin reagents that incorporate these enzymes. 

Importantly, this is a different formulation than our concentrated (5x) animal free blocker, due to the optimized conditions and neutral pH of the solution for IHC and IF methods. 

The post Animal-Free Blocker® and Diluent, R.T.U. appeared first on VectorLabs.

]]> Streptavidin, CY5 https://staging.vectorlabs.com/products/cy5-streptavidin/ Thu, 04 May 2023 04:12:37 +0000 https://staging.vectorlabs.com/?post_type=product&p=15026 Description

Vector Laboratories fluorochrome-conjugated streptavidin and avidin reagents are highly purified and possess very low non-specific binding properties. They have extremely high affinity for biotin. These fluorescent conjugates can be used to detect biotinylated secondary antibodies and other macromolecules in applications such as immunofluorescence, in situ hybridization, or flow cytometry.

Amplification of fluorescent signals can be easily achieved with our biotinylated secondary antibodies followed by our highly purified fluorochrome-labeled streptavidin or avidin. Using a biotin/avidin or biotin/streptavidin detection system results in an additional layer of amplification over a directly conjugated secondary antibody.

  • Excitation:  650 nm
  • Emission:  670 nm
  • Color:  Far Red

Specifications

Unit Size 1 mg
Applications Immunofluorescence, In situ hybridization, Flow Cytometry/Cell Separation
Recommended Usage Recommended concentration range for use 5-30 µg/ml Avoid using RPMI 1640 or other biotin-containing solutions as diluents.Serum also can contain biotin and should not be added to diluents.
Solution 10 mM HEPES, 0.15 M NaCl, pH 7.5, 0.08% sodium azide.
Maximum Excitation 647 nm
Maximum Emission 663 nm
Recommended Storage 2-8 °C
Concentration 1.0 mg/ml active conjugate
Conjugate Cy5
Color of Fluorescence Far Red
Format Concentrate

The post Streptavidin, CY5 appeared first on VectorLabs.

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Description

Vector Laboratories fluorochrome-conjugated streptavidin and avidin reagents are highly purified and possess very low non-specific binding properties. They have extremely high affinity for biotin. These fluorescent conjugates can be used to detect biotinylated secondary antibodies and other macromolecules in applications such as immunofluorescence, in situ hybridization, or flow cytometry.

Amplification of fluorescent signals can be easily achieved with our biotinylated secondary antibodies followed by our highly purified fluorochrome-labeled streptavidin or avidin. Using a biotin/avidin or biotin/streptavidin detection system results in an additional layer of amplification over a directly conjugated secondary antibody. 

  • Excitation:  650 nm
  • Emission:  670 nm
  • Color:  Far Red

Specifications

Unit Size1 mg
ApplicationsImmunofluorescence, In situ hybridization, Flow Cytometry/Cell Separation
Recommended UsageRecommended concentration range for use 5-30 µg/ml Avoid using RPMI 1640 or other biotin-containing solutions as diluents.Serum also can contain biotin and should not be added to diluents.
Solution10 mM HEPES, 0.15 M NaCl, pH 7.5, 0.08% sodium azide.
Maximum Excitation647 nm
Maximum Emission663 nm
Recommended Storage2-8 °C
Concentration1.0 mg/ml active conjugate
ConjugateCy5
Color of FluorescenceFar Red
FormatConcentrate

Documents

Citations

The post Streptavidin, CY5 appeared first on VectorLabs.

]]> ImmPRESS® Duet Double Staining Polymer Kit (HRP Anti-Mouse IgG-brown, AP Anti-Rabbit IgG-magenta) https://staging.vectorlabs.com/products/immpress-duet-double-staining-kit-red/ Tue, 02 May 2023 20:20:29 +0000 https://staging.vectorlabs.com/?post_type=product&p=14695 Description

This ImmPRESS Duet Double Staining Polymer Kit enables fast, well-defined localization and visualization of two different target antigens on the same tissue section. Utilizing our unique ImmPRESS micropolymer chemistry, we independently conjugate highly-active horseradish peroxidase (HRP) and alkaline phosphatase (AP) enzyme polymers to anti-mouse IgG and anti-rabbit IgG affinity-purified, highly cross-adsorbed secondary antibodies.

Features:

Save time and streamline your double staining IHC workflow with the ImmPRESS Duet Double Staining Polymer Kit.

  • Complete Kits for IHC Double Labeling
  • Convenient Dual Specificity (anti-mouse IgG/rabbit IgG) ImmPRESS HRP/AP Polymer Reagent
  • High sensitivity and Specificity
  • Non-Biotin Based Reagents
  • Prediluted, Ready-to-use

Kit Contents:

  • 15 ml BLOXALL® Blocking Solution
  • 15 ml 2.5% Normal Serum
  • 15 ml ImmPRESS Duet HRP/AP Polymer Reagent (mixture of ImmPRESS HRP Anti-Mouse IgG and ImmPRESS AP Anti-Rabbit IgG)
  • ImmPACT® DAB EqV Substrate (HRP, brown)
  • ImmPACT Vector® Red Substrate (AP, magenta)

Note: The ImmPRESS Duet Double Staining Polymer Kit is intended for use on non-rodent tissue specimens.

Specifications

Unit Size 1 kit
Applications Immunohistochemistry / Immunocytochemistry
Technology Micropolymer Reagents
Detection Enzyme(s) HRP/AP
Conjugate Micropolymer HRP/Micropolymer AP
Target Species Universal (Mouse/Rabbit)
Format Ready-to-Use
Blocking Action Endogenous Peroxidase, Endogenous Alkaline Phosphatase, Non-Specific Protein Blocking

 

The post ImmPRESS® Duet Double Staining Polymer Kit (HRP Anti-Mouse IgG-brown, AP Anti-Rabbit IgG-magenta) appeared first on VectorLabs.

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Description

 

This ImmPRESS Duet Double Staining Polymer Kit enables fast, well-defined localization and visualization of two different target antigens on the same tissue section. Utilizing our unique ImmPRESS micropolymer chemistry, we independently conjugate highly-active horseradish peroxidase (HRP) and alkaline phosphatase (AP) enzyme polymers to anti-mouse IgG and anti-rabbit IgG affinity-purified, highly cross-adsorbed secondary antibodies.

Features:

Save time and streamline your double staining IHC workflow with the ImmPRESS Duet Double Staining Polymer Kit.

  • Complete Kits for IHC Double Labeling
  • Convenient Dual Specificity (anti-mouse IgG/rabbit IgG) ImmPRESS HRP/AP Polymer Reagent
  • High sensitivity and Specificity
  • Non-Biotin Based Reagents
  • Prediluted, Ready-to-use

 

Kit Contents:

  • 15 ml BLOXALL® Blocking Solution
  • 15 ml 2.5% Normal Serum
  • 15 ml ImmPRESS Duet HRP/AP Polymer Reagent (mixture of ImmPRESS HRP Anti-Mouse IgG and ImmPRESS AP Anti-Rabbit IgG)
  • ImmPACT® DAB EqV Substrate (HRP, brown)
  • ImmPACT Vector® Red Substrate (AP, magenta)

 

Note: The ImmPRESS Duet Double Staining Polymer Kit is intended for use on non-rodent tissue specimens.

Specifications

Unit Size1 kit
ApplicationsImmunohistochemistry / Immunocytochemistry
TechnologyMicropolymer Reagents
Detection Enzyme(s)HRP/AP
ConjugateMicropolymer HRP/Micropolymer AP
Target SpeciesUniversal (Mouse/Rabbit)
FormatReady-to-Use
Blocking ActionEndogenous Peroxidase, Endogenous Alkaline Phosphatase, Non-Specific Protein Blocking

Documents

 

Technical Information

Save time and streamline your double staining IHC workflow with the ImmPRESS Duet Double Staining Polymer Kit. These reagents are blended at optimized dilutions to provide a stable pre-diluted, ready-to-use HRP/AP formula that will detect mouse and rabbit primary antibodies, and facilitate a time-saving, simplified, one-step double label detection protocol. For maximum sensitivity and staining contrast between target antigens, ImmPACT DAB EqV (HRP, brown) and ImmPACT Vector Red (AP, magenta) substrates are included to complete this easy to use staining kit.

Reliable and reproducible results are obtained on tissue sections where the two target antigens do not over-lap (co-localize) in the same structure of the same cell, but rather are expressed in different cell compartments or different cell types.

Also included, BLOXALL Blocking Solution abolishes all endogenous peroxidase and alkaline phosphatase activity in a single 10 minute incubation. In addition, 2.5% Normal Serum blocks all non-specific binding of detection reagents to ensure crisp, specific staining.

All reagents are supplied in stabilized, ready-to-use (R.T.U.) solutions for maximum convenience, and consistency between assays. The ImmPRESS Duet Double Staining Polymer Kits offer a complete and simple solution for visualizing target antigens in cell and tissue preparations and contains sufficient reagents to stain approximately 150 sections.

Product review on Biocompare.com.

The post ImmPRESS® Duet Double Staining Polymer Kit (HRP Anti-Mouse IgG-brown, AP Anti-Rabbit IgG-magenta) appeared first on VectorLabs.

]]> ImmPRESS® Duet Double Staining Polymer Kit (HRP – Anti-Rabbit IgG-brown, AP – Anti-Mouse IgG-magenta) https://staging.vectorlabs.com/products/immpress-duet-staining-kit-rb-br-ms-red/ Tue, 02 May 2023 20:12:52 +0000 https://staging.vectorlabs.com/?post_type=product&p=14675 Description

This ImmPRESS Duet Double Staining Polymer Kit enables fast, well-defined localization and visualization of two different target antigens on the same tissue section. Utilizing our unique ImmPRESS micropolymer chemistry, we independently conjugate highly-active horseradish peroxidase (HRP) and alkaline phosphatase (AP) enzyme polymers to anti-rabbit IgG and anti-mouse IgG affinity-purified, highly cross-adsorbed secondary antibodies.

Features:

Save time and streamline your double staining IHC workflow with the ImmPRESS Duet Double Staining Polymer Kit.

  • Complete Kits for IHC Double Labeling
  • Convenient Dual Specificity (anti-mouse IgG/rabbit IgG) ImmPRESS HRP/AP Polymer Reagent
  • High Sensitivity and Specificity
  • Non-Biotin Based Reagents
  • Prediluted, Ready-to-use

Kit Contents:

  • 15 ml BLOXALL® Blocking Solution
  • 15 ml 2.5% Normal Serum
  • 15 ml ImmPRESS Duet HRP/AP Polymer Reagent (mixture of ImmPRESS HRP Anti-Rabbit IgG and ImmPRESS  AP Anti-Mouse IgG)
  • ImmPACT® DAB EqV Substrate (HRP, brown)
  • ImmPACT Vector® Red Substrate (AP, magenta)

 

Note: The ImmPRESS Duet Double Staining Polymer Kit is intended for use on non-rodent tissue specimens.

Specifications

Unit Size 1 kit
Applications Immunohistochemistry / Immunocytochemistry
Technology Micropolymer Reagents
Detection Enzyme(s) HRP/AP
Conjugate Micropolymer HRP/Micropolymer AP
Target Species Universal (Mouse/Rabbit)
Format Ready-to-Use
Blocking Action Endogenous Peroxidase, Endogenous Alkaline Phosphatase, Non-Specific Protein Blocking

The post ImmPRESS® Duet Double Staining Polymer Kit (HRP – Anti-Rabbit IgG-brown, AP – Anti-Mouse IgG-magenta) appeared first on VectorLabs.

]]>

Description

 

This ImmPRESS Duet Double Staining Polymer Kit enables fast, well-defined localization and visualization of two different target antigens on the same tissue section. Utilizing our unique ImmPRESS micropolymer chemistry, we independently conjugate highly-active horseradish peroxidase (HRP) and alkaline phosphatase (AP) enzyme polymers to anti-rabbit IgG and anti-mouse IgG affinity-purified, highly cross-adsorbed secondary antibodies.

Features:

Save time and streamline your double staining IHC workflow with the ImmPRESS Duet Double Staining Polymer Kit.

  • Complete Kits for IHC Double Labeling
  • Convenient Dual Specificity (anti-mouse IgG/rabbit IgG) ImmPRESS HRP/AP Polymer Reagent
  • High Sensitivity and Specificity
  • Non-Biotin Based Reagents
  • Prediluted, Ready-to-use

 

Kit Contents:

  • 15 ml BLOXALL® Blocking Solution
  • 15 ml 2.5% Normal Serum
  • 15 ml ImmPRESS Duet HRP/AP Polymer Reagent (mixture of ImmPRESS HRP Anti-Rabbit IgG and ImmPRESS  AP Anti-Mouse IgG)
  • ImmPACT® DAB EqV Substrate (HRP, brown)
  • ImmPACT Vector® Red Substrate (AP, magenta)

Note: The ImmPRESS Duet Double Staining Polymer Kit is intended for use on non-rodent tissue specimens.

Specifications

Unit Size1 kit
ApplicationsImmunohistochemistry / Immunocytochemistry
TechnologyMicropolymer Reagents
Detection Enzyme(s)HRP/AP
ConjugateMicropolymer HRP/Micropolymer AP
Target SpeciesUniversal (Mouse/Rabbit)
FormatReady-to-Use
Blocking ActionEndogenous Peroxidase, Endogenous Alkaline Phosphatase, Non-Specific Protein Blocking

Documents

Technical Information

Save time and streamline your double staining IHC workflow with the ImmPRESS Duet Double Staining Polymer Kit.  These reagents are blended at optimized dilutions to provide a stable pre-diluted, ready-to-use HRP/AP formula that will detect mouse and rabbit primary antibodies, and facilitate a time-saving, simplified, one-step double label detection protocol. For maximum sensitivity and staining contrast between target antigens, ImmPACT DAB EqV (HRP, brown) and ImmPACT Vector® Red (AP, magenta) substrates are included to complete this easy to use staining kit.

Reliable and reproducible results are obtained on tissue sections where the two target antigens do not over-lap (co-localize) in the same structure of the same cell, but rather are expressed in different cell compartments or different cell types.

Also included, BLOXALL  Blocking Solution abolishes all endogenous peroxidase and alkaline phosphatase activity in a single 10 minute incubation. In addition, 2.5% Normal Serum blocks all non-specific binding of detection reagents to ensure crisp, specific staining.

All reagents are supplied in stabilized, ready-to-use (R.T.U.) solutions for maximum convenience, and consistency between assays. The ImmPRESS Duet Double Staining Polymer Kits offer a complete and simple solution for visualizing target antigens in cell and tissue preparations and contains sufficient reagents to stain approximately 150 sections.

Product review on Biocompare.com.

The post ImmPRESS® Duet Double Staining Polymer Kit (HRP – Anti-Rabbit IgG-brown, AP – Anti-Mouse IgG-magenta) appeared first on VectorLabs.

]]> VectaFluor™ Duet Immunofluorescence Double Labeling Kit, DyLight® 594 Anti-Rabbit (red), DyLight® 488 Anti-Mouse (green) https://staging.vectorlabs.com/products/vectafluor-duet-kit-mouse-gr-rabbit-red/ Tue, 02 May 2023 19:41:06 +0000 https://staging.vectorlabs.com/?post_type=product&p=14539 Description

VectaFluor Duet Kit offers maximum convenience to achieve immunofluorescence double labeling in one step. This kit includes a ready-to-use detection reagent that will detect mouse and rabbit primary antibodies with green and red DyLight fluorescent dyes in a single step.  Our affinity purified, extensively cross-adsorbed secondary antibodies are conjugated to DyLight dyes in a manner that ensures maximum degree of labeling without compromising antibody affinity or specificity. VectaFluor Duet is compatible with fluorescence staining of cells and tissues.

Features:

  • Robust cocktail formulation of DyLight anti-mouse IgG/anti-rabbit IgG secondary antibodies
  • Affinity-purified, ultrapure, high affinity antibodies

Specifications

Unit Size 1 kit
Applications Immunofluorescence
Target Species Universal (Mouse/Rabbit)
Conjugate DyLight 488/DyLight 594
Color of Fluorescence Kit (Green, Red)
Host Species Horse
Format Ready-to-Use

Kit Contents:

  • 15 ml 2.5% Normal Horse Serum (ready-to-use) for blocking
  • 15 ml VectaFluor Duet Reagent [DyLight 594 Anti-Rabbit IgG and DyLight 488 Anti-Mouse IgG cocktail (rabbit-red, mouse-green)] made in horse

The post VectaFluor™ Duet Immunofluorescence Double Labeling Kit, DyLight® 594 Anti-Rabbit (red), DyLight® 488 Anti-Mouse (green) appeared first on VectorLabs.

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Description

VectaFluor Duet Kit offers maximum convenience to achieve immunofluorescence double labeling in one step. This kit includes a ready-to-use detection reagent that will detect mouse and rabbit primary antibodies with green and red DyLight fluorescent dyes in a single step.  Our affinity purified, extensively cross-adsorbed secondary antibodies are conjugated to DyLight dyes in a manner that ensures maximum degree of labeling without compromising antibody affinity or specificity. VectaFluor Duet is compatible with fluorescence staining of cells and tissues.

Features:

  • Robust cocktail formulation of DyLight anti-mouse IgG/anti-rabbit IgG secondary antibodies
  • Affinity-purified, ultrapure, high affinity antibodies

Specifications

Unit Size1 kit
ApplicationsImmunofluorescence
Target SpeciesUniversal (Mouse/Rabbit)
ConjugateDyLight 488/DyLight 594
Color of FluorescenceKit (Green, Red)
Host SpeciesHorse
FormatReady-to-Use

Kit Contents:

  • 15 ml 2.5% Normal Horse Serum (ready-to-use) for blocking
  • 15 ml VectaFluor Duet Reagent [DyLight 594 Anti-Rabbit IgG and DyLight 488 Anti-Mouse IgG cocktail (rabbit-red, mouse-green)] made in horse

Documents

Technical Information

Kit Contents:

  • 15 ml 2.5% Normal Horse Serum (ready-to-use) for blocking 
  • 15 ml VectaFluor Duet Reagent [DyLight 594 Anti-Rabbit IgG and DyLight 488 Anti-Mouse IgG cocktail (rabbit-red, mouse-green)] made in horse, ready-to-use in 5% NHS. 

DyLight™ 488:

  • Excitation maximum:   493 nm
  • Emission maximum:  518 nm
  • Color:  Green

DyLight™ 594:

  • Excitation maximum:   592 nm
  • Emission maximum:  617 nm
  • Color:  Red

DyLight fluorescent dyes are direct alternatives to traditional fluorophores such as fluorescein (FITC) and rhodamine. The excitation and emission spectra parallel that of other commercially available fluorescent reagents allowing for easy substitution into an existing protocol without requiring any further instrumentation or filter sets.  

DyLight dyes offer a number of potential advantages including greater photostability and brighter fluorescence.  DyLight dyes are completely stable over a pH range of 4-9 making them compatible with many aqueous-based buffers and diluents. The DyLight dyes can be applied as single labels or in combination with other DyLight dyes and fluorophores as part of a multiple immunofluorescent antigen staining methodology.  The Dylight dyes currently offered are DyLight 488 (green), DyLight 549 (orange), DyLight 594 (red), and DyLight 649 (far red).

The post VectaFluor™ Duet Immunofluorescence Double Labeling Kit, DyLight® 594 Anti-Rabbit (red), DyLight® 488 Anti-Mouse (green) appeared first on VectorLabs.

]]> Streptavidin, CY3 https://staging.vectorlabs.com/products/cy3-streptavidin/ Tue, 02 May 2023 19:24:51 +0000 https://staging.vectorlabs.com/?post_type=product&p=14518 Description

Vector Laboratories fluorochrome-conjugated streptavidin and avidin reagents are highly purified and possess very low non-specific binding properties. They have extremely high affinity for biotin. These fluorescent conjugates can be used to detect biotinylated secondary antibodies and other macromolecules in applications such as immunofluorescence, in situ hybridization, or flow cytometry.

Amplification of fluorescent signals can be easily achieved with our biotinylated secondary antibodies followed by our highly purified fluorochrome-labeled streptavidin or avidin. Using a biotin/avidin or biotin/streptavidin detection system results in an additional layer of amplification over a directly conjugated secondary antibody.

  • Excitation:  552 nm
  • Emission:  565 nm
  • Color:  Red

Specifications

Unit Size 1 mg
Applications Immunofluorescence, In situ hybridization, Flow Cytometry/Cell Separation
Recommended Usage Recommended concentration range for use 5-30 µg/ml Avoid using RPMI 1640 or other biotin-containing solutions as diluents. Serum also can contain biotin and should not be added to diluents.
Solution 10 mM HEPES, 0.15 M NaCl, pH 7.5, 0.08% sodium azide.
Maximum Excitation 550 nm
Maximum Emission 570 nm
Recommended Storage 2-8 °C
Concentration 1.0 mg/ml active conjugate
Conjugate Cy3
Color of Fluorescence Red
Format Concentrate

The post Streptavidin, CY3 appeared first on VectorLabs.

]]>

Description

Vector Laboratories fluorochrome-conjugated streptavidin and avidin reagents are highly purified and possess very low non-specific binding properties. They have extremely high affinity for biotin. These fluorescent conjugates can be used to detect biotinylated secondary antibodies and other macromolecules in applications such as immunofluorescence, in situ hybridization, or flow cytometry.

Amplification of fluorescent signals can be easily achieved with our biotinylated secondary antibodies followed by our highly purified fluorochrome-labeled streptavidin or avidin. Using a biotin/avidin or biotin/streptavidin detection system results in an additional layer of amplification over a directly conjugated secondary antibody. 

  • Excitation:  552 nm
  • Emission:  565 nm
  • Color:  Red

Specifications

Unit Size1 mg
ApplicationsImmunofluorescence, In situ hybridization, Flow Cytometry/Cell Separation
Recommended UsageRecommended concentration range for use 5-30 µg/ml Avoid using RPMI 1640 or other biotin-containing solutions as diluents. Serum also can contain biotin and should not be added to diluents.
Solution10 mM HEPES, 0.15 M NaCl, pH 7.5, 0.08% sodium azide.
Maximum Excitation550 nm
Maximum Emission570 nm
Recommended Storage2-8 °C
Concentration1.0 mg/ml active conjugate
ConjugateCy3
Color of FluorescenceRed
FormatConcentrate

Documents

Citations

The post Streptavidin, CY3 appeared first on VectorLabs.

]]> Vector® TrueVIEW® Autofluorescence Quenching Kit https://staging.vectorlabs.com/products/trueview-autofluorescence-quenching-kit/ Mon, 01 May 2023 20:44:17 +0000 https://staging.vectorlabs.com/?post_type=product&p=13656 Description

The TrueVIEW Autofluorescence Quenching Kit provides a novel way to diminish unwanted autofluorescence from non-lipofuscin sources and dramatically improve signal-to-noise ratio. TrueVIEW removes unwanted autofluorescence in tissue sections due to aldehyde fixation, the presence of red blood cells, and structural elements such as collagen and elastin. The quenching action of the kit reagents provides a clear, unambiguous, “true view” localization of the target antigen.

This kit offers you:

  • Effectivity with problematic tissues such as kidney and spleen
  • An easy-to-use, one-step method
  • Compatibility with a wide selection of fluorophores
  • Compatibility with standard epifluorescence and confocal laser microscopes
  • Availability with DAPI counterstain and without.

One kit is sufficient to treat approximately 100 to 150 tissue sections.

Specifications

Unit Size 15 ml
Applications Immunofluorescence, In situ hybridization
Blocking Action Autofluorescence
Format Concentrate

Kit Contents:

What’s in the box?

  • TrueVIEW Reagent A, 5 ml
  • TrueVIEW Reagent B, 5 ml
  • TrueVIEW Reagent C, 5 ml
  • VECTASHIELD Vibrance® Antifade Mounting Medium, 2 ml

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Description

The TrueVIEW Autofluorescence Quenching Kit provides a novel way to diminish unwanted autofluorescence from non-lipofuscin sources and dramatically improve signal-to-noise ratio. TrueVIEW removes unwanted autofluorescence in tissue sections due to aldehyde fixation, the presence of red blood cells, and structural elements such as collagen and elastin. The quenching action of the kit reagents provides a clear, unambiguous, “true view” localization of the target antigen.

This kit offers you:

  • Effectivity with problematic tissues such as kidney and spleen
  • An easy-to-use, one-step method
  • Compatibility with a wide selection of fluorophores
  • Compatibility with standard epifluorescence and confocal laser microscopes
  • Availability with DAPI counterstain and without.

One kit is sufficient to treat approximately 100 to 150 tissue sections. 

Specifications

Unit Size15 ml
ApplicationsImmunofluorescence, In situ hybridization
Blocking ActionAutofluorescence
FormatConcentrate

Kit Contents:

What’s in the box?

  • TrueVIEW Reagent A, 5 ml
  • TrueVIEW Reagent B, 5 ml
  • TrueVIEW Reagent C, 5 ml
  • VECTASHIELD Vibrance® Antifade Mounting Medium, 2 ml

Documents

Product FAQs

During product development, applying the TrueVIEW™ Quenching reagent at the end of our standard IF procedure yielded the most optimal reduction in autofluorescence signal. The reagent is retained on the tissue at the time of mounting, allowing for extended quenching action, with little to no effect on the specific fluorescent signal. However, in applications with very brief staining procedures, such as a primary antibody directly conjugated with a fluorophore, application of TrueVIEW™ Quenching reagent may be just as effective at the start of the procedure.

Yes, there are a number of published references describing the use of TrueVIEW Autofluorescence Quenching Kit in the scientific literature. Please refer to a partial list of these publications in the Technical Information section of the product detail page for SP-8400.

The working solution of TrueVIEW works in an electrostatic manner to greatly reduce or eliminate fluorescence in tissue sections induced through the use of an aldehyde based fixative. TrueVIEW is also effective at reducing fluorescence from tissue components such as collagen, elastin and red blood cells. TureVIEW does not work to reduce autofluorescence due to lipofuscin.

Once made up, the working solution of TrueVIEW can be stored either on the bench top or in the fridge (2-8C) fro about 48 hours (2 days) withut loss of activity or function. Following this time we would suggest discarding unused working solution and make fresh solution as required.

Yes, the working solution is a blue color that does “stain” the tissue section blue. This indicates an active and appropriate chemical reaction is occurring. The blue stain on the section does not fluoresce and does not interfere with the immunofluorescence application.

Both TrueVIEW products, SP-8400 and SP-8500, are supplied with 2 mL of VECTASHIELD Vibrance antifade mounting media. We have found that the mounting media does play a crucial role in maintaining the high signal to noise ratio when using TrueVIEW. VECTASHIELD Vibrance is included therefore as a critical component of the kit and it is recommended to use the media supplied for optimal results. At this time we do not have sufficient data to confidently recommend the use of other vendors mounting media with TrueVIEW. Substitution of VECTASHIELD Vibrance with another mounting media may result in less than satisfactory results.

No, we have found that the TrueVIEW reagent lifts off the tissue using TBS or HEPES buffer. Detergents are incompatible.

Citations

Technical Information

Tissue autofluorescence often occurs with aldehyde fixation or from inherent native tissue components (collagen, elastin, and red blood cells). The extent and intensity of autofluorescence background frequently makes it difficult or impossible to distinguish specific signals in immunofluorescence applications.

Most methods for reduction of tissue autofluorescence act primarily on lipofuscin granules, and are not broadly effective against the most common sources of autofluorescence targeted by TrueVIEW Quencher.

Current methods for reducing autofluorescence primarily include “home brew” concoctions such as sodium borohydride and other ink-based products. These methods are essentially ineffective against aldehyde induced autofluorescence. In contrast, TrueVIEW reagent binds to hydrophilic compounds and effectively quenches endogenous autofluorescence.

 
Spleen2 jpg
 

Image showing Spleen FFPE tissue stain, with & without TrueVIEW autofluorescence quencer. Antigen retrieved with Antigen Unmasking Solution. Spleen tissue stained with Mouse Anti-CD20 (red) and Rabbit Anti-Ki67 (green), followed by VectaFluor™ Duet Kit, and mounted in VECTASHIELD Vibrance Mounting Medium with DAPI.

Left Image:  No TrueVIEW Quencher treatment – 20x objective, red channel exposed 200ms, green channel 200ms. Note presence of interfering autofluorescence.

Right Image: Cells treated with TrueVIEW Autofluorescence Quencher – 20x objective, red channel exposed 200ms, green channel exposed 800ms. Note absence of autofluorescence.

Human tonsil (FFPE); Section stained for AE1/AE3 using fluorescein label (green). Treated with TrueVIEW and mounted with VECTASHIELD Vibrance Antifade Mounting Medium with DAPI (nuclei blue).
 
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Image showing Pancreas FFPE tissue stain. Antigen retrieved with Antigen Unmasking Solution, stained with Guinea Pig x Insulin (green) and Mouse x END (red), followed by FL-Goat x Guinea Pig IgG + DyLight™ 594-Horse x Mouse IgG. Mounted in VECTASHIELD Vibrance Mounting Medium with DAPI.

Left Image: No TrueVIEW Quencher used, 20x objective, red channel exposed 600ms, green channel 600ms.

Right Image: TrueVIEW Quencher used with 20x objective, red channel exposed 600ms, green channel exposed 600ms.

Easy To Apply

Following completion of the IF staining procedure:

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Mode of Action

Following completion of the IF staining procedure:

TVaction jpg

Signal to Noise Optimization

The primary antibody and detection reagents should be optimized (titered) in conjunction with Vector TrueVIEW Quenching Kit to achieve maximum signal to noise.

TVprimary opt jpg

Optimization of Exposure

Increasing exposure times may be necessary to achieve optimal image acquisition for TrueVIEW Quencher treated slides.

TVexp opt600 jpg

Comparison Between Vector TrueVIEW Reagent and Other Autofluorescence Reducing Agents

We compared the effectiveness of Vector TrueVIEW quenching action in parallel with other commercially available autofluorescence reducing products and “home brew” reagents, on serial sections of formalin-fixed, paraffin-embedded human pancreas visualized using a standard fluorescein (green) filter. No specific immunofluorescence staining was conducted. The images below highlight our results. All images were acquired under identical conditions (including microscope objective and exposure times).

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TVtimecourse jpg

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]]> VECTASHIELD® PLUS Antifade Mounting Medium https://staging.vectorlabs.com/products/vectashield-plus-antifade/ Mon, 01 May 2023 15:29:14 +0000 https://staging.vectorlabs.com/?post_type=product&p=13487 Description

VECTASHIELD PLUS protects against fading across the visible spectrum, even under far-red wavelengths. It is compatible with most commercially available fluorophores. This new formulation of non-setting media improves upon the original VECTASHIELD products. Specifically, VECTASHIELD PLUS exhibits no inherent background or toning and provides superior fluorophore signal retention across the spectrum, including far-red wavelengths.

Features:

  • No inherent toning or background
  • Superior signal retention across the spectrum from blue to far red
  • Mounted sections can be viewed immediately
  • Available in two convenient sizes
  • Superior non-setting formulation

 

Specifications

Unit Size 2 ml, 10 ml
Applications Immunofluorescence, In situ hybridization
Recommended Storage 2-8 ºC
Mounting Aqueous (Non-Hardening)
Antifade Yes
Counterstain None

Technical Information

The refractive index for VECTASHIELD Mounting Medium is 1.45.

VECTASHIELD PLUS Mounting Medium does not solidify, but rather, remains a liquid on the slide—which can then be stored without sealing. If desired, coverslips can be sealed around the perimeter using nail polish or plastic sealant. Mounted slides should be stored at 2-8 °C and protected from light.

VECTASHIELD Mounting Medium Antifade Comparison

Other manufacturers measure the antifade properties of their mountants using labeled microspheres or arrayed spots. Vector Labs prefers to measure antifade properties of VECTASHIELD mountants using frozen tissue sections that have been immunohistochemically stained with fluorescently labeled secondary antibodies. Antifade capability is then measured using a 40x objective with real time imaging over 30 seconds of continuous exposure to the excitation illumination. Individual intensity measurements are recorded from 6 separate labeled regions and the average is calculated. The intensity after 30 second exposure is expressed as a percentage of the intensity at zero time. The values for PG are taken from the manufacturer’s published results.

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Description

VECTASHIELD PLUS protects against fading across the visible spectrum, even under far-red wavelengths. It is compatible with most commercially available fluorophores. This new formulation of non-setting media improves upon the original VECTASHIELD products. Specifically, VECTASHIELD PLUS exhibits no inherent background or toning and provides superior fluorophore signal retention across the spectrum, including far-red wavelengths.

Features:

  • No inherent toning or background
  • Superior signal retention across the spectrum from blue to far red
  • Mounted sections can be viewed immediately
  • Available in two convenient sizes
  • Superior non-setting formulation

Specifications

Unit Size2 ml, 10 ml
ApplicationsImmunofluorescence, In situ hybridization
Recommended Storage2-8 ºC
MountingAqueous (Non-Hardening)
AntifadeYes
CounterstainNone

Documents

Product FAQ

VECTASHIELD PLUS provides several advantages over the regular VECTASHIELD (H-1000 & H-1200). In particular, VECTASHIELD PLUS media have significantly lower inherent background​ toning and provide superior signal retention particularly in the far-red spectrum (e.g. Cy5).

Similar to other non-setting media, we recommend you to seal the coverslip perimeter if the intention is to store the slides for a period of time.

Yes, the antifade mounting media are at least equivalent in signal retention. We have not seen any reduction in performance ​with any fluorophores evaluated, and with some dyes (i.e. Alexa Fluor 647 and Dylight 649) there are advantages. 

We do know that regular VECTASHIELD antifade mounting media (H-1000 & H-1200) are suitable for SRM.​ However, the formulation for VECTASHIELD PLUS is different from that used for ​regular VECTASHIELD. Indeed one component of H-1000 and​ H-1200 that may have been a contributing factor to the underlying blue​ tone and that appears to play a key role in optimal signal for SRM, has​ been removed from VECTASHIELD PLUS. Therefore, until we have data to the ​contrary VECTASHIELD PLUS would not be recommended for SRM.

Technical Information

The refractive index for VECTASHIELD Mounting Medium is 1.45.

VECTASHIELD PLUS Mounting Medium does not solidify, but rather, remains a liquid on the slide—which can then be stored without sealing. If desired, coverslips can be sealed around the perimeter using nail polish or plastic sealant. Mounted slides should be stored at 2-8 °C and protected from light.

VECTASHIELD Mounting Medium Antifade Comparison

Other manufacturers measure the antifade properties of their mountants using labeled microspheres or arrayed spots. Vector Labs prefers to measure antifade properties of VECTASHIELD mountants using frozen tissue sections that have been immunohistochemically stained with fluorescently labeled secondary antibodies. Antifade capability is then measured using a 40x objective with real time imaging over 30 seconds of continuous exposure to the excitation illumination. Individual intensity measurements are recorded from 6 separate labeled regions and the average is calculated. The intensity after 30 second exposure is expressed as a percentage of the intensity at zero time. The values for PG are taken from the manufacturer’s published results.

New Fluorophore Signal Table VECTASHIELD jpg New Fluoro Remaining Graph jpg VS.Chart .v2 jpg

Resources

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How to mount a coverslip on a slide

Correctly coverslipping your slides ensures that your tissue specimens are preserved for optimal imaging and viewing. In this blog post, we will walk you through how to properly coverslip your slides to protect your samples.

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How to choose antifade mounting media

As far as antifade mounting media go, one-size-fits-all solutions are few and far between. Here, we break down key factors you should consider when choosing the best antifade mounting medium for your experiment.

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Step by Step Protocol
v2 VL LIT3018 VECTASHIELD PLUS Brochure Cover 1 jpg
Product-specific information

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]]> H.O.H.™ (Human on Human) Immunodetection Kit https://staging.vectorlabs.com/products/human-on-human-immunodetection-kit/ Sun, 30 Apr 2023 02:41:03 +0000 https://staging.vectorlabs.com/?post_type=product&p=13399 Description

The H.O.H. Immunodetection Kit is intended to detect human (or humanized) antibodies on frozen or paraffin embedded human tissue sections.

Key advantages of the H.O.H. Immunodetection Kit:

  • Clear, crisp, specific staining of antigens of interest
  • Procedures are simple and easy to follow
  • No tedious calculations are required

 

The H.O.H. Immunodetection Kit contains enough stock reagents to produce about 5 ml of working solution, which is generally sufficient to stain approximately 50 - 100 tissue sections.

 

Specifications

Unit Size 1 kit
Applications Immunohistochemistry / Immunocytochemistry, Blotting Applications
Technology Human on Human (H.O.H.) Systems
Detection Enzyme(s) Peroxidase
Target Species Human
Format Concentrate
Blocking Action Endogenous Human Ig

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Resources

Description

The H.O.H. Immunodetection Kit is intended to detect human (or humanized) antibodies on frozen or paraffin embedded human tissue sections.

Key advantages of the H.O.H. Immunodetection Kit:

  • Clear, crisp, specific staining of antigens of interest
  • Procedures are simple and easy to follow
  • No tedious calculations are required

 

The H.O.H. Immunodetection Kit contains enough stock reagents to produce about 5 ml of working solution, which is generally sufficient to stain approximately 50 – 100 tissue sections.

Specifications

Unit Size1 kit
ApplicationsImmunohistochemistry / Immunocytochemistry, Blotting Applications
TechnologyHuman on Human (H.O.H.) Systems
Detection Enzyme(s)Peroxidase
Target SpeciesHuman
FormatConcentrate
Blocking ActionEndogenous Human Ig

Kit Components

What’s in the box:

  • 5 ml Protein Block
  • 2.5 ml Solution A
  • 2.5 ml Solution B
  • 300 µl HRP Anti-Goat IgG
  • 5 ml HRP Antibody Diluent
  • 2.5 ml ImmPACT DAB EqV Reagent 1 (Chromogen)
  • 2.5 ml ImmPACT DAB EqV Reagent 2 (Diluent)

Documents

Technical Information

The H.O.H. Immunodetection Kit is intended to detect human (or humanized) antibodies on frozen or paraffin embedded human tissue sections.

The inability of a secondary antibody to distinguish between a primary antibody produced in humans and the endogenous human immunoglobulins present in human tissue results in high background staining, which obscures specific staining. This problem can be eliminated by using our Human on Human Kit and the result is clear, crisp, specific staining of the antigens of interest.

This kit employs a straightforward two-step primary antibody preparation followed by standard IHC assay detection procedures. Once the human primary antibody solution has been prepared, assay time is approximately 90 minutes.

This comprehensive kit includes all required volume-matched reagents including ImmPACT DAB EqV peroxidase substrate. The procedures for use are easy to follow and omit the necessity of tedious and often confusing calculations. The H.O.H. Immunodetection Kit can be applied to most cell and tissue based staining situations where the detection of endogenous human IgG would be problematic when using a human, humanized, or chimeric human primary antibody. This kit is effective in eliminating confounding interference from endogenous human IgG on highly antigenic frozen or paraffin embedded tissue sections.

HOH 2 mockup 1600x1000px

Download our HOH-3000 Whitepaper:

Optimized Human on Human Immunodetection

 

 

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