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Vector® Blue Substrate Kit, Alkaline Phosphatase (AP) (SK-5300)



The Vector Blue AP Substrate Kit produces a blue, heat stable reaction product. This chromogen can be used singly or in combination with other alkaline phosphatase or peroxidase substrates for multiple label applications.


  • Greater sensitivity than conventional substrates
  • Consistent and reliable
  • Ideal for IHC/ICC/ISH and blots
  • Fluorescent
  • Heat Stable
  • Permanent mounting
  • Stock solutions supplied in convenient dropper bottles promoting ease of handling
  • No wait times for mixing and dissolving powders or tablets
  • Sufficient reagents to produce 200 ml of working solution
  • Excitation: broad, peak at ~500 nm*
  • Emission: broad, peak at ~680 nm*


*Histochem J. (1998) 8:577


Unit Size1 kit
ApplicationsImmunohistochemistry / Immunocytochemistry, Immunofluorescence, In situ hybridization, Blotting Applications, Elispot
Detection Enzyme(s)Alkaline Phosphatase
Substrate Reaction ProductPrecipitating Reaction Product
Chromogen ColorBlue
Color of FluorescenceFar Red
MicroscopyBrightfield, Fluorescence, Spectral Imaging
Additional Substrate PropertiesContrast with Tissue Pigments, Fluorescent, Heat Resistant, Multiple Labeling
MountingAqueous (Hardening), Aqueous (Non-Hardening), Non-Aqueous (Permanent)
Safety TitleWARNING: Reproductive Harm –

Kit Contents:

  • 4 ml Vector Blue Reagent 1
  • 4 ml Vector Blue Reagent 2
  • 3 ml Vector Blue Reagent 3


Technical Information


Vector Blue is both chromogenic and fluorescent. Sections stained with the Vector Blue substrate can be dehydrated, cleared, and permanently mounted; however, non-xylene clearing agents and non-xylene based mounting media (such as VectaMount®, H-5000) must be used because the reaction product is soluble in xylene. Vector Blue can also be used on blots in applications such as ELISPOT, singly or in combination with a peroxidase substrate such as AEC. This kit contains stock solutions in convenient dropper bottles.

SUBSTRATEsensitivity2 jpg  Substrate.CounterstainChart jpg

SubstrateCombos jpg

Applicable patents and legal notices are available at legal notices.

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