Description
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Description
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Specifications
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Documents
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Product FAQs
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Citations
- Technical Information
Description
Tomato lectin, although sharing some specificities with potato lectin, Datura lectin, and wheat germ agglutinin, has been reported to be dissimilar in many respects. LEL binds well to glycophorin and Tamm-Horsfall glycoprotein and has been used effectively to label vascular endothelium in rodents.
Texas Red® labeled Tomato lectin has an appropriate number of fluorochromes bound to provide the optimum staining characteristics for this lectin. This conjugate is supplied essentially free of unconjugated fluorochromes. The excitation maximum is at 595 nm and the emission maximum is at 615 nm.
Specifications
| Unit Size | 1 mg |
|---|---|
| Applications | Immunofluorescence, Glycobiology |
| Recommended Usage | If a precipitate forms upon long-term storage, warm to 37 ºC. |
| Recommended Storage | 2-8 °C |
| Maximum Excitation | 595-604 nm |
| Maximum Emission | 606-615 nm |
| Solution | 10 mM HEPES, 0.15 M NaCl, pH 7.5, 0.08% sodium azide 0.1 mM CaCl2. |
| Concentration | 1 mg active conjugate/ml |
| Conjugate | Texas Red |
| Color of Fluorescence | Red |
| Sugar Specificity | [GlcNAc]1-3, N-Acetylglucosamine |
Documents
Product FAQs
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Can the biotinylated Lycopersicon esculentum (tomato) lectin be used for in vivo perfusion studies to trace blood vessels in mice?
The Lycopersicon esculentum (tomato) lectin has been widely reported as an effective blood vessel marker for in vivo vascular perfusion studies in rodent species. Investigators have primarily utilized one of the fluorophore conjugated tomato lectin formats to trace blood vasculature in normal and diseased animals via tail vein or intracardiac injection. However, the biotinylated format has also been used. It allows for flexibility in subsequent visualization by way of either fluorescence or enzyme-based methods. Published references are best source for procedural details. Examples of references where biotinylated tomato lectins have been applied via in vivo perfusion are featured below: Robertson, R.T., et al. (2014) Histochem. Cell Biol. 143(2) Thurston, G., et al. (1998) Am. J Pathol. 153(4):1099-1112
Citations
112
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Technical Information
Tomato lectin is a very stable single subunit glycoprotein containing about 50 percent arabinose and galactose and may form multimeric aggregates in solution.
Tomato lectin (from Lycopersicon esculentum) is an effective marker of blood vessels and microglial cells in rodents. Conjugation of the lectin with a fluorophore facilitates fast, one-step detection and visualization using intravascular perfusion methods or direct application to tissue sections.
Texas Red® or DyLight™ 594 conjugated tomato lectin provides an excellent contrast to green/yellow fluorescence such as GFP expressed in transgenic animals, or with fluorescein conjugates in standard double label studies. The tomato lectin complements our existing range of lectin reagents and should be a valuable tool in examining rodent tumor angiogenesis, tracing neovascular development in xenograft models and brain research.
Accompanying each fluorescent lectin is an analysis data sheet summarizing the results of our quality control tests and providing pertinent information on the product. All of these reagents are supplied as solutions preserved with sodium azide.
Inhibiting/Eluting Sugar: Chitin Hydrolysate
