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NEUROBIOTIN® 488 Tracer (SP-1125-2)

The product has been discontinued

Please consider the following as potential substitutes: SP-1120-20. NEUROBIOTIN Tracer. Or contact Technical Support @ [email protected] for assistance in finding alternative.


NEUROBIOTIN 488 Tracer is a tri-functional molecule designed for neuronal tracing and cell filling.


  • Bright green fluorophore, similar in fluorescence to fluorescein, Cy2 or Alexa Fluor® 488
  • Biotin label with a biotinidase-resistant linkage
  • Fixable primary amine
  • Used for visualizing neural architecture and for the identification of gap junction coupling
  • Can be used in many types of preparations including in vivo, whole mounts, slice preparations, or cultured cells
  • Can be delivered by many routes such as intracellular electrodes, microinjection, cut-loading, or scrape-loading


Advantages of NEUROBIOTIN Tracer over biocytin and other neuronal labels:

  • Better solubility
  • More efficiently iontophoresed
  • Remains in cell longer
  • Non-toxic
  • Can be fixed with formalin or glutaraldehyde


Unit Size2 mg
Maximum Emission517 nm
Recommended Storage2-8 °C (desiccated). Once in solution, store frozen. This product does not contain an antimicrobial agent.
Recommended UsageNEUROBIOTIN 488 is soluble under the following conditions.Solvents and concentrations will require optimization depending on the application. Up to 10% in 3.0 M Tris, pH 8.8 Up to 2% in 0.25 M Tris, pH 8.8 or 0.25 M Tris, 1.0 M NaCl, pH 8.8 Up to 2% in 0.1 M bicarbonate buffer, pH 8.5 or 0.25 M bicarbonate, 1.0 M NaCl, pH 8.5 Up to 0.5% in 0.13 M cesium gluconate, 10 mM HEPES, pH 7.2 Up to 10% in DMSO
Molecular Weight747 g/mol
Maximum Excitation493 nm
Neuronal Tracer – Direction of TransportAnterograde/Retrograde
Detection MethodAvidin(Streptavidin)/Biotin Method, Chromogenic, Fluorescence


Technical Information

NEUROBIOTIN 488 Tracer is transported along axons and fine projections and is compatible with patch clamp applications and pressure injection methods. NEUROBIOTIN 488 Tracer is readily fixed with standard aldehyde based fixatives. This tracer can be viewed directly under fluorescence microscopy. 

Applicable patents and legal notices are available at legal notices.