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ImmPACT® Vector® Red Substrate Kit, Alkaline Phosphatase (AP) (SK-5105)



ImmPACT Vector Red AP Substrate Kit produces a magenta reaction product that is crisper and is 2-4 times more sensitive than the original Vector® Red substrate kit (SK-5100). It can be used singly or in combination with other alkaline phosphatase or peroxidase substrates for multiple label applications.


  • Ideal for IHC/ICC/ISH and blots
  • Fluorescent
  • Heat Stable
  • Permanent mounting
  • Supplied as 120 ml of ready-to-use diluent and stock solutions in dropper bottles
  • Excitation: broad excitation spectrum, approx. 365 nm to 560 nm
  • Emission: Above 560 nm
  • Color: Red


Unit Size1 kit
ApplicationsImmunohistochemistry / Immunocytochemistry, Immunofluorescence, In situ hybridization, Blotting Applications, Elispot
Detection Enzyme(s)Alkaline Phosphatase
Substrate Reaction ProductPrecipitating Reaction Product
Chromogen ColorMagenta
Color of FluorescenceRed
MicroscopyBrightfield, Fluorescence, Spectral Imaging
Additional Substrate PropertiesContrast with Tissue Pigments, Fluorescent, Heat Resistant, Multiple Labeling
MountingAqueous (Hardening), Aqueous (Non-Hardening), Non-Aqueous (Permanent)

Kit Contents:

  • 120 ml ImmPACT Vector Red Diluent
  • 2.5 ml ImmPACT Vector Red Reagent 1
  • 2.5 ml ImmPACT Vector Red Reagent 2


Technical Information

Both Vector Red and ImmPACT Vector Red alkaline phosphatase (AP) substrates produce red, heat stable reaction products that are also highly fluorescent and non-fading. Preparations stained with either Vector Red or ImmPACT Vector Red AP chromogen can be dehydrated, cleared, and permanently mounted. Because of their stability under ISH conditions, both chromogens can be used in IHC/ISH double labeling applications. Both formulations can be used for either manual or automated staining methods.

The bright fluorescence of the Vector Red or ImmPACT Vector Red reaction products are visible using DyLight 594, Texas Red® or TRITC filter systems. The fluorescence is especially bright in dehydrated, permanently mounted sections. The fluorescence is stable and does not photobleach like most fluorescent dyes.

Either substrate formulation can also be used on blots. Compared to other AP substrates, the Vector Red and ImmPACT Vector Red reaction products have a wider dynamic range, offering better quantitation of targets on blots.

SUBSTRATEsensitivity2 jpg

Substrate.CounterstainChart jpg SubstrateCombos jpg

Applicable patents and legal notices are available at legal notices.

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