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AQuora® 488-NHS Ester is an amine-reactive fluorophore engineered with SuperHydrophilic™ technology designed to improve solubility during labeling and of the dye labeled conjugate. As a result, the dye labeled conjugates made with AQuora® 488-NHS Ester yield enhanced signal and signal-to-noise ratios in fluorescence-based applications including fluorescent western blotting, fluorescence-based microscopy, flow cytometry, and cell-based assays. AQuora® 488-NHS Ester is a xanthene-based dye with photophysical properties comparable to Alexa Fluor® 488 & DyLight® 488.
This AQuora® 488-NHS Ester dye is activated with a N-hydroxysuccinimide (NHS) ester group which reacts with free primary amines, such as the amines on the side chain of lysines, forming a stable amide bond.
Alexa Fluor® and DyLight® are registered trademarks of Thermo Fisher Scientific.
Unit Size | 1mg |
---|---|
Format | Solid |
Storage | Store at -20°C. Protect from light. May ship at ambient temperatures. |
Country of Origin | United States of America |
Label | AQuora® 488 |
Excitation / Emission Maximum | 493 nm / 522 nm |
A280 Correction Factor | 0.110 |
Reactive Group | NHS Ester |
Extinction Coefficient | 73,000 |
Spectrally Similar Dyes | Alexa Fluor® 488 & DyLight® 488 |
Molecular Weight | 1250 |
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