Category: Specialty Products – VectorLabs https://staging.vectorlabs.com From linker design and synthesis, bioconjugated design and manufacturing, biomolecule labeling and functionalization, and detection system and solutions, our team is here to help you move forward, with impact. Wed, 22 Oct 2025 17:15:31 +0000 en-US hourly 1 https://wordpress.org/?v=6.9 https://staging.vectorlabs.com/wp-content/uploads/2024/11/V-favicon-02-100x100.png Category: Specialty Products – VectorLabs https://staging.vectorlabs.com 32 32 VectaCell™ Trolox Antifade Reagent for Live Cell Imaging https://staging.vectorlabs.com/products/vectacell-trolox-antifade-reagent/ Tue, 02 May 2023 16:00:15 +0000 https://staging.vectorlabs.com/?post_type=product&p=14307 Description

VectaCell Trolox is an antifading additive for live cell imaging. It is a stock solution of the antioxidant Trolox (6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid) in filtered ethanol. Trolox is a water-soluble and cell-permeable analog of vitamin E that efficiently prevents formation of various reactive oxygen species, such as singlet oxygen (O2), superoxide anion (O2) or hydrogen peroxide (H2O2).

Specifications

Unit Size 2 ml
Applications Immunofluorescence, Cellular Imaging
Concentration 100 mM (in ethanol)
Recommended Usage Instructions for use: Dilute the Trolox Solution into culture media or imaging buffer to a final concentration between 0.1 mM and 1 mM. Notes: The optimal working concentration of Trolox in imaging media will depend on the cell type and their tolerance to hypoxia. For cells that have low tolerance to hypoxia, or can be affected by the addition of ethanol, the appropriate controls should be included in the assay.
Recommended Storage 2-8 °C
Microscopy Fluorescence
Antifade Yes

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Description

VectaCell Trolox is an antifading additive for live cell imaging. It is a stock solution of the antioxidant Trolox (6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid) in filtered ethanol. Trolox is a water-soluble and cell-permeable analog of vitamin E that efficiently prevents formation of various reactive oxygen species, such as singlet oxygen (O2), superoxide anion (O2) or hydrogen peroxide (H2O2).

Specifications

Unit Size2 ml
ApplicationsImmunofluorescence, Cellular Imaging
Concentration100 mM (in ethanol)
Recommended UsageInstructions for use: Dilute the Trolox Solution into culture media or imaging buffer to a final concentration between 0.1 mM and 1 mM. Notes: The optimal working concentration of Trolox in imaging media will depend on the cell type and their tolerance to hypoxia. For cells that have low tolerance to hypoxia, or can be affected by the addition of ethanol, the appropriate controls should be included in the assay.
Recommended Storage2-8 °C
MicroscopyFluorescence
AntifadeYes

Documents

Technical Information

VectaCell Trolox contains both Trolox and its oxidized form Trolox-quinone. This system reduces photo-bleaching and blinking during live cell imaging,1, 2 and has also been shown to have a cytoprotective effect and low cytotoxicity for different cell lines.3

Trolox has been used to reduce fluorophore bleaching1 as well as the fluorophore blinking2 during the observation of fluorescent labels. Although the mechanism is not completely understood, it has been attributed to the combination of its oxygen-scavenger capacity that prevents oxidative bleaching and the complementary reduction reaction that helps prevent blinking.

VectaCell Trolox is supplied as a convenient 100 mM stock solution.

References:

  1. Rasnik et al. Nonblinking and long-lasting single-molecule fourorescence imaging. (2006) Nat. Meth. 3:891-893
  2. Cordes et al. On the Mechanism of Trolox as Antiblinking and Antibleaching Reagent. (2009) J. Am. Chem. Soc. 131:5018-5019
  3. Wu et al. The cytoprotective effect of Trolox demonstrated with three types of human cells. (1990) Biochem. Cell Biol. 68: 1189-1194

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]]> NEUROBIOTIN® Tracer https://staging.vectorlabs.com/products/neurobiotin-tracer/ Tue, 02 May 2023 15:56:09 +0000 https://staging.vectorlabs.com/?post_type=product&p=14294 Description

NEUROBIOTIN Tracer (SP-1120) is an amino derivative of biotin that can be used as an intracellular label for cells, particularly neurons. It is used for visualizing neural architecture and for the identification of gap junction coupling.

Features:

  • Can be used in many types of preparations including in vivo, whole mounts, slice preparations, or cultured cells
  • Can be delivered by many routes such as intracellular electrodes, microinjection, cut-loading, or scrape-loading
  • Can be detected using avidin or streptavidin systems with either chromogenic or fluorescence visualization methods

Advantages over biocytin and other neuronal labels:

  • Better solubility
  • More efficiently iontophoresed
  • Remains in cell longer
  • Non-toxic
  • Can be fixed with formalin or glutaraldehyde

Specifications

Unit Size 20 mg, 50 mg
Chemical Formula C12H23ClN4O2S
Recommended Storage 2-8 °C (desiccated). Once in solution, store frozen.
Molecular Weight 322.8
Neuronal Tracer – Direction of Transport Anterograde/Retrograde
Detection Method Avidin(Streptavidin)/Biotin Method, Chromogenic, Fluorescence

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Description

NEUROBIOTIN Tracer (SP-1120) is an amino derivative of biotin that can be used as an intracellular label for cells, particularly neurons. It is used for visualizing neural architecture and for the identification of gap junction coupling.

Features:

  • Can be used in many types of preparations including in vivo, whole mounts, slice preparations, or cultured cells
  • Can be delivered by many routes such as intracellular electrodes, microinjection, cut-loading, or scrape-loading
  • Can be detected using avidin or streptavidin systems with either chromogenic or fluorescence visualization methods

 

Advantages over biocytin and other neuronal labels:

  • Better solubility
  • More efficiently iontophoresed
  • Remains in cell longer
  • Non-toxic
  • Can be fixed with formalin or glutaraldehyde

Specifications

Unit Size20 mg, 50 mg
Chemical FormulaC12H23ClN4O2S
Recommended Storage2-8 °C (desiccated). Once in solution, store frozen.
Molecular Weight322.8
Neuronal Tracer – Direction of TransportAnterograde/Retrograde
Detection MethodAvidin(Streptavidin)/Biotin Method, Chromogenic, Fluorescence

Documents

Citations

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]]> NEUROBIOTIN® 350 Tracer https://staging.vectorlabs.com/products/neurobiotin-350/ Tue, 02 May 2023 00:21:00 +0000 https://staging.vectorlabs.com/?post_type=product&p=13778 Description

NEUROBIOTIN 350 Tracer is a tri-functional molecule designed for neuronal tracing and cell filling.

Features:

  • Bright blue fluorophore, similar in fluorescence to AMCA or Alexa Fluor® 350
  • Biotin label with a biotinidase-resistant linkage
  • Fixable primary amine
  • Used for visualizing neural architecture and for the identification of gap junction coupling
  • Can be used in many types of preparations including in vivo, whole mounts, slice preparations, or cultured cells
  • Can be delivered by many routes such as intracellular electrodes, microinjection, cut-loading, or scrape-loading
  • Biotin label can be detected using avidin or streptavidin systems with either chromogenic or fluorescence visualization methods

Advantages of NEUROBIOTIN Tracer over biocytin and other neuronal labels:

  • Better solubility
  • More efficiently iontophoresed
  • Remains in cell longer
  • Non-toxic

Specifications

Unit Size 2 mg
Maximum Emission 452 nm
Recommended Storage 2-8 °C (desiccated). Once in solution, store frozen. This product does not contain an antimicrobial agent.
Recommended Usage NEUROBIOTIN 350 is soluble at >5% in the following: water, 1M potassium acetate, 1 M KCl, 1 M NaCl, 1 M Tris, pH 8.5, and 100 mM Tris, pH 7.5.Solubility in phosphate buffers is significantly lower.Solvents and concentrations will require optimization depending on the application.It may be important to note that unbuffered NEUROBIOTIN 350 is somewhat acidic and may require buffering for some applications. NEUROBIOTIN 350 is a tri-functional molecule designed for neuronal tracing and cell filling that contains: •a biotin label with a biotinidase-resistant linker. •a bright fluorophore, similar in fluorescence to AMCA or Alexa Fluor® 350. •a fixable primary amino group. Fixable with formaldehyde or glutaraldehyde
Molecular Weight 573 g/mol
Maximum Excitation 346 nm
Neuronal Tracer – Direction of Transport Anterograde/Retrograde
Detection Method Avidin(Streptavidin)/Biotin Method, Chromogenic, Fluorescence

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Description

NEUROBIOTIN 350 Tracer is a tri-functional molecule designed for neuronal tracing and cell filling.

Features:

  • Bright blue fluorophore, similar in fluorescence to AMCA or Alexa Fluor® 350
  • Biotin label with a biotinidase-resistant linkage
  • Fixable primary amine
  • Used for visualizing neural architecture and for the identification of gap junction coupling
  • Can be used in many types of preparations including in vivo, whole mounts, slice preparations, or cultured cells
  • Can be delivered by many routes such as intracellular electrodes, microinjection, cut-loading, or scrape-loading
  • Biotin label can be detected using avidin or streptavidin systems with either chromogenic or fluorescence visualization methods

 

Advantages of NEUROBIOTIN Tracer over biocytin and other neuronal labels:

  • Better solubility
  • More efficiently iontophoresed
  • Remains in cell longer
  • Non-toxic
  • Can be fixed with formalin or glutaraldehyde

Specifications

Unit Size2 mg
Maximum Emission452 nm
Recommended Storage2-8 °C (desiccated). Once in solution, store frozen. This product does not contain an antimicrobial agent.
Recommended UsageNEUROBIOTIN 350 is soluble at >5% in the following: water, 1M potassium acetate, 1 M KCl, 1 M NaCl, 1 M Tris, pH 8.5, and 100 mM Tris, pH 7.5.Solubility in phosphate buffers is significantly lower.Solvents and concentrations will require optimization depending on the application.It may be important to note that unbuffered NEUROBIOTIN 350 is somewhat acidic and may require buffering for some applications. NEUROBIOTIN 350 is a tri-functional molecule designed for neuronal tracing and cell filling that contains: •a biotin label with a biotinidase-resistant linker. •a bright fluorophore, similar in fluorescence to AMCA or Alexa Fluor® 350. •a fixable primary amino group. Fixable with formaldehyde or glutaraldehyde
Molecular Weight573 g/mol
Maximum Excitation346 nm
Neuronal Tracer – Direction of TransportAnterograde/Retrograde
Detection MethodAvidin(Streptavidin)/Biotin Method, Chromogenic, Fluorescence

Documents

Citations

Technical Information

Similar in structure to NEUROBIOTIN 488, NEUROBIOTIN 350 is also an amine containing biotin derivative. However, it is conjugated with a bright blue fluorophore that would contrast with other green or red fluorescent markers or reagents present in the same preparation. For administration purposes NEUROBIOTIN 350 has a net positive charge and is highly soluble in a number of commonly used buffer and salt solutions.

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]]> NEUROBIOTIN®-PLUS Tracer https://staging.vectorlabs.com/products/neurobiotin-plus/ Tue, 02 May 2023 00:15:53 +0000 https://staging.vectorlabs.com/?post_type=product&p=13768 Description

NEUROBIOTIN-Plus Tracer is a biotin label that is impervious to cleavage and breakdown by biotinidase. It is used for visualizing neural architecture and for the identification of gap junction coupling

Features:

  • Fixable primary amine
  • Can be used in many types of preparations including in vivo, whole mounts, slice preparations, or cultured cells
  • Can be delivered by many routes such as intracellular electrodes, microinjection, cut-loading, or scrape-loading
  • Biotin label can be detected using avidin or streptavidin systems with either chromogenic or fluorescence visualization methods

Advantages of NEUROBIOTIN Tracer over biocytin and other neuronal labels:

  • Better solubility
  • More efficiently iontophoresed
  • Remains in cell longer
  • Non-toxic
  • Can be fixed with formalin or glutaraldehyde

Specifications

Unit Size 5 mg
Recommended Storage 2-8 °C (desiccated). Once in solution, store frozen. This product does not contain an antimicrobial agent.
Recommended Usage Fixable with formaldehyde or glutaraldehyde
Solution 10% solutions can be made in water, buffers, 1 M KCl, 1 M NaCl, or dimethylsulfoxide.
Molecular Weight 358 g/mol
Neuronal Tracer – Direction of Transport Anterograde/Retrograde
Detection Method Avidin(Streptavidin)/Biotin Method, Chromogenic, Fluorescence

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Description

NEUROBIOTIN-Plus Tracer is a biotin label that is impervious to cleavage and breakdown by biotinidase. It is used for visualizing neural architecture and for the identification of gap junction coupling

Features:

  • Fixable primary amine
  • Can be used in many types of preparations including in vivo, whole mounts, slice preparations, or cultured cells
  • Can be delivered by many routes such as intracellular electrodes, microinjection, cut-loading, or scrape-loading
  • Biotin label can be detected using avidin or streptavidin systems with either chromogenic or fluorescence visualization methods

 

Advantages of NEUROBIOTIN Tracer over biocytin and other neuronal labels:

  • Better solubility
  • More efficiently iontophoresed
  • Remains in cell longer
  • Non-toxic
  • Can be fixed with formalin or glutaraldehyde

Specifications

Unit Size5 mg
Recommended Storage2-8 °C (desiccated). Once in solution, store frozen. This product does not contain an antimicrobial agent.
Recommended UsageFixable with formaldehyde or glutaraldehyde
Solution10% solutions can be made in water, buffers, 1 M KCl, 1 M NaCl, or dimethylsulfoxide.
Molecular Weight358 g/mol
Neuronal Tracer – Direction of TransportAnterograde/Retrograde
Detection MethodAvidin(Streptavidin)/Biotin Method, Chromogenic, Fluorescence

Documents

Citations

Technical Information

Investigators have identified rapid degradation of injected tracers such as biocytin due to the presence of biotinidase activity in brain tissue*. This loss of stability of the tracer significantly reduces the viable postinjection period and may compromise complete detection of a neural network. NEUROBIOTIN-Plus was designed to be impervious to cleavage and breakdown by biotinidase. This newly synthesized biotin-containing compound contains fixable amine groups, is highly soluble and has a low molecular weight. These characteristics make it an ideal candidate for experiments requiring long postinjection survival times and optimal uptake along the entire neural tract.

NEUROBIOTIN-Plus shares the same key advantages of NEUROBIOTIN Tracer over biocytin and other neuronal labels:

  • Better solubility
  • More efficiently iontophoresed
  • Remains in cell longer
  • Non-toxic
  • Can be fixed with formalin or glutaraldehyde/li>

NEUROBIOTIN-Plus can be detected using avidin or streptavidin systems with either chromogenic or fluorescence visualization methods.

*Mishra, A. et al (2010) ACS Chem. Neurosci. 1:129-138.

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]]> NEUROBIOTIN® 488 Tracer https://staging.vectorlabs.com/products/neurobiotin-488/ Tue, 02 May 2023 00:08:57 +0000 https://staging.vectorlabs.com/?post_type=product&p=13758 Description

NEUROBIOTIN 488 Tracer is a tri-functional molecule designed for neuronal tracing and cell filling.

Features:

  • Bright green fluorophore, similar in fluorescence to fluorescein, Cy2 or Alexa Fluor® 488
  • Biotin label with a biotinidase-resistant linkage
  • Fixable primary amine
  • Used for visualizing neural architecture and for the identification of gap junction coupling
  • Can be used in many types of preparations including in vivo, whole mounts, slice preparations, or cultured cells
  • Can be delivered by many routes such as intracellular electrodes, microinjection, cut-loading, or scrape-loading

Advantages of NEUROBIOTIN Tracer over biocytin and other neuronal labels:

  • Better solubility
  • More efficiently iontophoresed
  • Remains in cell longer
  • Non-toxic

Specifications

Unit Size 2 mg
Maximum Emission 517 nm
Recommended Storage 2-8 °C (desiccated). Once in solution, store frozen. This product does not contain an antimicrobial agent.
Recommended Usage NEUROBIOTIN 488 is soluble under the following conditions.Solvents and concentrations will require optimization depending on the application. Up to 10% in 3.0 M Tris, pH 8.8 Up to 2% in 0.25 M Tris, pH 8.8 or 0.25 M Tris, 1.0 M NaCl, pH 8.8 Up to 2% in 0.1 M bicarbonate buffer, pH 8.5 or 0.25 M bicarbonate, 1.0 M NaCl, pH 8.5 Up to 0.5% in 0.13 M cesium gluconate, 10 mM HEPES, pH 7.2 Up to 10% in DMSO
Molecular Weight 747 g/mol
Maximum Excitation 493 nm
Neuronal Tracer – Direction of Transport Anterograde/Retrograde
Detection Method Avidin(Streptavidin)/Biotin Method, Chromogenic, Fluorescence

The post NEUROBIOTIN® 488 Tracer appeared first on VectorLabs.

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Description

NEUROBIOTIN 488 Tracer is a tri-functional molecule designed for neuronal tracing and cell filling.

Features:

  • Bright green fluorophore, similar in fluorescence to fluorescein, Cy2 or Alexa Fluor® 488
  • Biotin label with a biotinidase-resistant linkage
  • Fixable primary amine
  • Used for visualizing neural architecture and for the identification of gap junction coupling
  • Can be used in many types of preparations including in vivo, whole mounts, slice preparations, or cultured cells
  • Can be delivered by many routes such as intracellular electrodes, microinjection, cut-loading, or scrape-loading

 

Advantages of NEUROBIOTIN Tracer over biocytin and other neuronal labels:

  • Better solubility
  • More efficiently iontophoresed
  • Remains in cell longer
  • Non-toxic
  • Can be fixed with formalin or glutaraldehyde

Specifications

Unit Size2 mg
Maximum Emission517 nm
Recommended Storage2-8 °C (desiccated). Once in solution, store frozen. This product does not contain an antimicrobial agent.
Recommended UsageNEUROBIOTIN 488 is soluble under the following conditions.Solvents and concentrations will require optimization depending on the application. Up to 10% in 3.0 M Tris, pH 8.8 Up to 2% in 0.25 M Tris, pH 8.8 or 0.25 M Tris, 1.0 M NaCl, pH 8.8 Up to 2% in 0.1 M bicarbonate buffer, pH 8.5 or 0.25 M bicarbonate, 1.0 M NaCl, pH 8.5 Up to 0.5% in 0.13 M cesium gluconate, 10 mM HEPES, pH 7.2 Up to 10% in DMSO
Molecular Weight747 g/mol
Maximum Excitation493 nm
Neuronal Tracer – Direction of TransportAnterograde/Retrograde
Detection MethodAvidin(Streptavidin)/Biotin Method, Chromogenic, Fluorescence

Documents

Citations

Technical Information

NEUROBIOTIN 488 Tracer is transported along axons and fine projections and is compatible with patch clamp applications and pressure injection methods. NEUROBIOTIN 488 Tracer is readily fixed with standard aldehyde based fixatives. This tracer can be viewed directly under fluorescence microscopy. 

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]]> Wheat Germ Agglutinin (WGA), Peroxidase https://staging.vectorlabs.com/products/peroxidase-conjugated-wheat-germ-agglutinin/ Fri, 28 Apr 2023 19:51:54 +0000 https://staging.vectorlabs.com/?post_type=product&p=12841 Description

Peroxidase conjugated Wheat Germ Agglutinin is specially prepared for neuronal transport studies. This product is supplied as 40 mg/ml of protein in a 2 mg package ready for injection. After tissue preparation, detection can be readily achieved with a peroxidase substrate.

Specifications

Unit Size 2 mg
Applications Immunohistochemistry / Immunocytochemistry, Neurobiology, Blotting Applications, ELISAs, Glycobiology
Conjugate Peroxidase
Sugar Specificity N-Acetylglucosamine
Safety Title WARNING: Cancer – www.P65Warnings.ca.gov

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Description

Peroxidase conjugated Wheat Germ Agglutinin is specially prepared for neuronal transport studies. This product is supplied as 40 mg/ml of protein in a 2 mg package ready for injection. After tissue preparation, detection can be readily achieved with a peroxidase substrate.

Specifications

Unit Size2 mg
ApplicationsImmunohistochemistry / Immunocytochemistry, Neurobiology, Blotting Applications, ELISAs, Glycobiology
ConjugatePeroxidase
Sugar SpecificityN-Acetylglucosamine
Safety TitleWARNING: Cancer – www.P65Warnings.ca.gov

Documents

Citations

Technical Information

Wheat germ agglutinin (WGA) contains a group of closely related isolectins, with an isoelectric point about pH 9. The receptor sugar for WGA is N-acetylglucosamine, with preferential binding to dimers and trimers of this sugar. WGA can bind oligosaccharides containing terminal N-acetylglucosamine or chitobiose, structures which are common to many serum and membrane glycoproteins. Bacterial cell wall peptidoglycans, chitin, cartilage glycosaminoglycans, and glycolipids can also bind WGA. Native WGA has also been reported to interact with some glycoproteins via sialic acid residues (see succinylated WGA). This lectin is used for the purification of insulin receptors and for neuronal tracing.

WGA-HRP can be used for both anterograde and retrograde tracing. Our peroxidase-wheat germ agglutinin is specially prepared for neuronal transport studies. WGA-peroxidase features a high concentration of protein and is supplied in a 2 mg package at 40 mg/ml ready for injection. Unconjugated wheat germ agglutinin, also used in neuronal studies is available as 10 mg and 25 mg of salt-free, lyophilized lectin.

Inhibiting/Eluting Sugar: Chitin Hydrolysate or 500 mM N-acetylglucosamine with salt and/or acid elution generally required

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]]> Phaseolus Vulgaris Leucoagglutinin (PHA-L), Unconjugated https://staging.vectorlabs.com/products/unconj-phaseolus-vulgaris-leucoagglutinin/ Thu, 27 Apr 2023 14:16:22 +0000 https://staging.vectorlabs.com/?post_type=product&p=12389 Description

Phaseolus vulgaris agglutinin is the name ascribed to a family of lectins, each of which consists of four subunits. There are two different types of subunits. One appears to be involved primarily in red cell agglutination and has been designated the “E” subunit (for erythroagglutinin). The other type is involved in lymphocyte agglutination and mitogenic activity and has been termed the “L” subunit (for leucoagglutinin). These subunits combine to produce five isolectins.

The unconjugated PHA-L has been found to be an excellent, specific marker for use in anterograde neuronal tracing

Specifications

Unit Size 5 mg
Applications Immunohistochemistry / Immunocytochemistry, Immunofluorescence, Blotting Applications, Glycobiology, Mitogenic Stimulation
Recommended Usage Reconstitution: The lectin should be reconstituted 10 mM phosphate, pH 8.0. If reconstituted in 1 ml of 10 mM phosphate, the resulting solution will be 5 mM phosphate, 10 mM NaCl with trace CaCl2. After reconstitution, and if appropriate for use, a preservative such as sodium azide (0.04%) may be added. If the lectin is to be used for mitogenic assays, filter solution through a sterile 0.22 µm filter immediately after reconstitution, aliquot and store frozen. NOTE: Mitogenic activity may be diminished by repeated freezing and thawing. Anterograde Neuronal Tracing: For neuronal transport studies, reconstitute in 0.2 ml of 10 mM sodium phosphate, pH 8.0.Both the pH and ionic strength of the reconstituting buffer are important for optimal anterograde transport. A specific protocol for this application is available on request.
Recommended Storage 2-8 °C
Conjugate Unconjugated
Sugar Specificity Galactose, Complex Structures

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Description

Phaseolus vulgaris agglutinin is the name ascribed to a family of lectins, each of which consists of four subunits. There are two different types of subunits. One appears to be involved primarily in red cell agglutination and has been designated the “E” subunit (for erythroagglutinin). The other type is involved in lymphocyte agglutination and mitogenic activity and has been termed the “L” subunit (for leucoagglutinin). These subunits combine to produce five isolectins. 

The unconjugated PHA-L has been found to be an excellent, specific marker for use in anterograde neuronal tracing

Specifications

Unit Size5 mg
ApplicationsImmunohistochemistry / Immunocytochemistry, Immunofluorescence, Blotting Applications, Glycobiology, Mitogenic Stimulation
Recommended UsageReconstitution: The lectin should be reconstituted 10 mM phosphate, pH 8.0. If reconstituted in 1 ml of 10 mM phosphate, the resulting solution will be 5 mM phosphate, 10 mM NaCl with trace CaCl2. After reconstitution, and if appropriate for use, a preservative such as sodium azide (0.04%) may be added. If the lectin is to be used for mitogenic assays, filter solution through a sterile 0.22 µm filter immediately after reconstitution, aliquot and store frozen. NOTE: Mitogenic activity may be diminished by repeated freezing and thawing. Anterograde Neuronal Tracing: For neuronal transport studies, reconstitute in 0.2 ml of 10 mM sodium phosphate, pH 8.0.Both the pH and ionic strength of the reconstituting buffer are important for optimal anterograde transport. A specific protocol for this application is available on request.
Recommended Storage2-8 °C
ConjugateUnconjugated
Sugar SpecificityGalactose, Complex Structures

Documents

Citations

Technical Information

PHA-L, with four L type subunits, does not agglutinate red cells but is a potent mitogen. The other three isolectins, designated E3L1, E2L2, and E1L3, have erythroagglutinating and mitogenic activities proportional to the number of respective E or L subunits. We have termed the mixture of the five isolectins PHA (E+L).

After iontophoretic injection of unconjugated PHA-L, the approximate rate of anterograde transport is about 4-6 mm/day, with survival periods of over 18 days having been observed. Once transported, the unconjugated PHA-L is best visualized for light microscopy with antibody to the lectin, followed by the appropriate detection system and substrate. Fluorescent staining can also be used for a faster staining procedure. A complete applications protocol is available upon request.

Elution: 100 mM acetic acid

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