Peroxidase – VectorLabs https://staging.vectorlabs.com From linker design and synthesis, bioconjugated design and manufacturing, biomolecule labeling and functionalization, and detection system and solutions, our team is here to help you move forward, with impact. Wed, 22 Oct 2025 17:15:31 +0000 en-US hourly 1 https://wordpress.org/?v=6.9 https://staging.vectorlabs.com/wp-content/uploads/2024/11/V-favicon-02-100x100.png Peroxidase – VectorLabs https://staging.vectorlabs.com 32 32 WestVision™ Peroxidase Polymer, Anti-Mouse IgG (Western Blot Detection) https://staging.vectorlabs.com/products/westvision-hrp-polymer-anti-mouse-igg/ Tue, 02 May 2023 02:16:48 +0000 https://staging.vectorlabs.com/?post_type=product&p=13907 Description

Highly active WestVision™ Peroxidase Polymer antibody conjugates are western blot detection reagents. These reagents are:

  • Sensitive
  • Convenient
  • Economical

    WestVision™ Peroxidase Polymer antibody conjugates bind primary antibodies of mouse (WB-2000) or rabbit (WB-1000) origin with high affinity, producing sharp signal and low background in western blot detection. The conjugates are based on a new method of polymerizing enzymes and attaching these polymers to antibodies. The novel approach employed to form enzyme polymers avoids the use of large dextrans or other macromolecules as a backbone. Attaching these unique polymers to an antibody allows a higher density of enzymes to access a target with minimal interference. The result is an excellent signal-to-noise-ratio in western blot detection.

Specifications

Unit Size 0.8 ml
Applications Blotting Applications, Elispot
Recommended Usage Suggested working dilution range: Chromogenic substrates: 1:500-1:5000 Chemiluminescent substrates: 1:5,000-1:200,000
Solution 50 mM HEPES, 100 mM Na2SO4, pH 6.8, preservative.
Recommended Storage 2-8 °C, DO NOT FREEZE
Concentration 5.0 mg/ml conjugate
Technology Micropolymer Reagents
Detection Enzyme(s) Peroxidase
Conjugate Micropolymer HRP
Target Species Mouse
Host Species Horse
Format Concentrate

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Description

Highly active WestVision™ Peroxidase Polymer antibody conjugates are western blot detection reagents. These reagents are:

  • Sensitive
  • Convenient
  • Economical

    WestVision™ Peroxidase Polymer antibody conjugates bind primary antibodies of mouse (WB-2000) or rabbit (WB-1000) origin with high affinity, producing sharp signal and low background in western blot detection. The conjugates are based on a new method of polymerizing enzymes and attaching these polymers to antibodies. The novel approach employed to form enzyme polymers avoids the use of large dextrans or other macromolecules as a backbone. Attaching these unique polymers to an antibody allows a higher density of enzymes to access a target with minimal interference. The result is an excellent signal-to-noise-ratio in western blot detection.

Specifications

Unit Size0.8 ml
ApplicationsBlotting Applications, Elispot
Recommended UsageSuggested working dilution range: Chromogenic substrates: 1:500-1:5000 Chemiluminescent substrates: 1:5,000-1:200,000
Solution50 mM HEPES, 100 mM Na2SO4, pH 6.8, preservative.
Recommended Storage2-8 °C, DO NOT FREEZE
Concentration5.0 mg/ml conjugate
TechnologyMicropolymer Reagents
Detection Enzyme(s)Peroxidase
ConjugateMicropolymer HRP
Target SpeciesMouse
Host SpeciesHorse
FormatConcentrate

Documents

Citations

The post WestVision™ Peroxidase Polymer, Anti-Mouse IgG (Western Blot Detection) appeared first on VectorLabs.

]]> WestVision™ Peroxidase Polymer, Anti-Rabbit IgG (Western Blot Detection) https://staging.vectorlabs.com/products/westvision-hrp-polymer-anti-rabbit-igg/ Tue, 02 May 2023 02:12:20 +0000 https://staging.vectorlabs.com/?post_type=product&p=13900 Description

Highly active WestVision™ Peroxidase Polymer antibody conjugates have been added to our line of western blot detection reagents. These reagents are:

  • Sensitive
  • Convenient
  • Economical

    WestVision™ Peroxidase Polymer antibody conjugates bind primary antibodies of mouse (WB-2000) or rabbit (WB-1000) origin with high affinity, producing sharp signal and low background in western blot detection. The conjugates are based on a new method of polymerizing enzymes and attaching these polymers to antibodies. The novel approach employed to form enzyme polymers avoids the use of large dextrans or other macromolecules as a backbone. Attaching these unique polymers to an antibody allows a higher density of enzymes to access a target with minimal interference. The result is an excellent signal-to-noise- ratio in western blot detection.

Specifications

Unit Size 0.8 ml
Applications Blotting Applications, Elispot
Recommended Usage Suggested working dilution range: Chromogenic substrates: 1:500-1:5000 Chemiluminescent substrates: 1:5,000-1:200,000
Solution 50 mM HEPES, 100 mM Na2SO4, pH 6.8, preservative.
Recommended Storage 2-8 °C, DO NOT FREEZE
Concentration 5.0 mg/ml conjugate
Technology Micropolymer Reagents
Detection Enzyme(s) Peroxidase
Conjugate Micropolymer HRP
Target Species Rabbit
Host Species Horse
Format Concentrate

The post WestVision™ Peroxidase Polymer, Anti-Rabbit IgG (Western Blot Detection) appeared first on VectorLabs.

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Description

Highly active WestVision™ Peroxidase Polymer antibody conjugates have been added to our line of western blot detection reagents. These reagents are:

  • Sensitive
  • Convenient
  • Economical

    WestVision™ Peroxidase Polymer antibody conjugates bind primary antibodies of mouse (WB-2000) or rabbit (WB-1000) origin with high affinity, producing sharp signal and low background in western blot detection. The conjugates are based on a new method of polymerizing enzymes and attaching these polymers to antibodies. The novel approach employed to form enzyme polymers avoids the use of large dextrans or other macromolecules as a backbone. Attaching these unique polymers to an antibody allows a higher density of enzymes to access a target with minimal interference. The result is an excellent signal-to-noise- ratio in western blot detection.

Specifications

Host SpeciesHorse

Unit Size0.8 ml
ApplicationsBlotting Applications, Elispot
Recommended UsageSuggested working dilution range: Chromogenic substrates: 1:500-1:5000 Chemiluminescent substrates: 1:5,000-1:200,000
Solution50 mM HEPES, 100 mM Na2SO4, pH 6.8, preservative.
Recommended Storage2-8 °C, DO NOT FREEZE
Concentration5.0 mg/ml conjugate
TechnologyMicropolymer Reagents
Detection Enzyme(s)Peroxidase
ConjugateMicropolymer HRP
Target SpeciesRabbit
FormatConcentrate

Documents

Citations

Technical Information

Highly active WestVision™ Peroxidase Polymer antibody conjugates have been added to our line of western blot detection reagents. These reagents are:

  • Sensitive
  • Convenient
  • Economical

WestVision™ Peroxidase Polymer antibody conjugates bind primary antibodies of mouse (WB-2000) or rabbit (WB-1000) origin with high affinity, producing sharp signal and low background in western blot detection. The conjugates are based on a new method of polymerizing enzymes and attaching these polymers to antibodies. The novel approach employed to form enzyme polymers avoids the use of large dextrans or other macromolecules as a backbone. Attaching these unique polymers to an antibody allows a higher density of enzymes to access a target with minimal interference. The result is an excellent signal-to-noise- ratio in western blot detection.

The post WestVision™ Peroxidase Polymer, Anti-Rabbit IgG (Western Blot Detection) appeared first on VectorLabs.

]]> Goat Anti-Biotin, Peroxidase Conjugated https://staging.vectorlabs.com/products/goat-anti-biotin-peroxidase-conjugated/ Tue, 02 May 2023 01:10:55 +0000 https://staging.vectorlabs.com/?post_type=product&p=13819 Description

Peroxidase conjugated anti-biotin antibodies are produced with the highest specific acitivity enzymes in optimal enzyme/antibody ratios. The covalent linkage between the enzyme and antibody is chosen to provide stable, highly active conjugates for immunohistochemical, in situ hybridization, enzyme immunoassay, or transfer blot applications.

Specifications

Unit Size 1 mg
Applications Immunohistochemistry / Immunocytochemistry, Immunofluorescence, In situ hybridization, Blotting Applications, Elispot, ELISAs
Concentration 1 mg active conjugate/ml
Recommended Storage 2-8 °C, DO NOT FREEZE
Solution 10 mM sodium phosphate, pH 7.5, 0.15 M NaCl, 0.05% thimerosal
Recommended Usage Moles peroxidase/mole IgG:3.3 Recommended diluent for microtitration plate assays: 10 mM sodium phosphate, 0.15 M NaCl, pH 7.5, 0.1% Tween 20.Working concentration of HRP anti-biotin is 0.5 – 5.0 µg/ml. Do not use sodium azide with this product since it is an inhibitor of horseradish peroxidase.
Detection Target Biotin
Conjugate Peroxidase
Host Species Goat
Format Concentrate
Safety Title WARNING: Cancer – www.P65Warnings.ca.gov

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Description

Peroxidase conjugated anti-biotin antibodies are produced with the highest specific acitivity enzymes in optimal enzyme/antibody ratios. The covalent linkage between the enzyme and antibody is chosen to provide stable, highly active conjugates for immunohistochemical, in situ hybridization, enzyme immunoassay, or transfer blot applications.

Specifications

Unit Size1 mg
ApplicationsImmunohistochemistry / Immunocytochemistry, Immunofluorescence, In situ hybridization, Blotting Applications, Elispot, ELISAs
Concentration1 mg active conjugate/ml
Recommended Storage2-8 °C, DO NOT FREEZE
Solution10 mM sodium phosphate, pH 7.5, 0.15 M NaCl, 0.05% thimerosal
Recommended UsageMoles peroxidase/mole IgG:3.3 Recommended diluent for microtitration plate assays: 10 mM sodium phosphate, 0.15 M NaCl, pH 7.5, 0.1% Tween 20.Working concentration of HRP anti-biotin is 0.5 – 5.0 µg/ml. Do not use sodium azide with this product since it is an inhibitor of horseradish peroxidase.
Detection TargetBiotin
ConjugatePeroxidase
Host SpeciesGoat
FormatConcentrate
Safety TitleWARNING: Cancer – www.P65Warnings.ca.gov

Documents

Citations

Technical Information

Our high affinity polyclonal anti-biotin antibody is produced by hyperimmunizing goats with biotin and is purified by affinity chromatography. Anti-Biotin is available unconjugated, bound to heat stable, cross-linked agarose beads or labeled with enzymes or fluorescein. Agarose bound Anti-Biotin contains 1 mg of Anti-Biotin per ml of settled 4% agarose gel. This product can be used to bind and dissociate biotinylated molecules. Fluorescein labeled Anti-Biotin has been optimally conjugated to provide a bright fluorescent signal. The horseradish peroxidase and alkaline phosphatase conjugates have been produced with the highest specific activity enzymes in optimal enzyme/antibody ratios. The covalent linkage between the enzyme and antibody was chosen to provide stable, highly active conjugates for immunohistochemical, enzyme immunoassay, or transfer blot applications.

The post Goat Anti-Biotin, Peroxidase Conjugated appeared first on VectorLabs.

]]> DuoLuX® Chemiluminescent and Fluorescent Substrate, Peroxidase (HRP) https://staging.vectorlabs.com/products/duolux-chemiluminescent-and-fluorescent-hrp/ Mon, 01 May 2023 23:40:50 +0000 https://staging.vectorlabs.com/?post_type=product&p=13724 Description

The DuoLuX Chemiluminescent/Chemifluorescent Substrate can be used for western, Southern, northern, or dot blots, or for ELISA applications. This substrate is about 10 times more sensitive than the alkaline phosphatase substrate BCIP/NBT and about 100 times more sensitive than the peroxidase substrate DAB. In addition to its chemiluminescent properties, the reaction product of the DuoLuX Substrate is also fluorescent.

Features:

  • Unique formula based on acridan chemistry
  • Very high sensitivity
  • Prolonged and intense light emission
  • Permanent fluorescence
  • Luminescence – peak emission at 453 nm
  • Fluorescence – excitation maximum at 405 nm (also excites at 254 nm and 365 nm). Maximum fluorescence emission at 453nm.

Specifications

Unit Size 200 ml
Applications Blotting Applications, Elispot, ELISAs
Detection Enzyme(s) Peroxidase
Substrate Reaction Product Chemiluminescent/ Chemifluorescent Product

What’s in the box:

  • 100 ml DuoLuX Substrate (Reagent 1)
  • 100 ml Peroxidase Converter Solution (Reagent 2)

The post DuoLuX® Chemiluminescent and Fluorescent Substrate, Peroxidase (HRP) appeared first on VectorLabs.

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Description

The DuoLuX Chemiluminescent/Chemifluorescent Substrate can be used for western, Southern, northern, or dot blots, or for ELISA applications. This substrate is about 10 times more sensitive than the alkaline phosphatase substrate BCIP/NBT and about 100 times more sensitive than the peroxidase substrate DAB. In addition to its chemiluminescent properties, the reaction product of the DuoLuX Substrate is also fluorescent.

Features:

  • Unique formula based on acridan chemistry
  • Very high sensitivity
  • Prolonged and intense light emission
  • Permanent fluorescence
  • Luminescence – peak emission at 453 nm
  • Fluorescence – excitation maximum at 405 nm (also excites at 254 nm and 365 nm). Maximum fluorescence emission at 453nm.

Specifications

Unit Size200 ml
ApplicationsBlotting Applications, Elispot, ELISAs
Detection Enzyme(s)Peroxidase
Substrate Reaction ProductChemiluminescent/ Chemifluorescent Product

What’s in the box:

  • 100 ml DuoLuX Substrate (Reagent 1)
  • 100 ml Peroxidase Converter Solution (Reagent 2)

Documents

Citations

Technical Information

Contents:

The DuoLuX Chemiluminescent/Chemifluorescent Substrate for peroxidase (SK-6604) is supplied in two bottles, consisting of 100 ml of the DuoLuX Substrate (Reagent 1) and 100 ml of a peroxidase converter solution (Reagent 2). Reagents 1 and 2 are mixed in equal volumes just prior to use, providing 200 ml of peroxidase substrate working solution.

Chemiluminescent properties

Light emission of the DuoLuX Chemiluminescent/ Chemifluorescent Substrate occurs continuously over a long period of time for both the peroxidase and alkaline phosphatase substrates. With either substrate formula, reacted DuoLuX Substrate luminesces in the blue range with a peak emission at 453 nm. Unlike other chemiluminescent substrates, blots can be reexposed to film many times over an 8 hour period to optimize band intensities or resolution.

DLchemilumlifetime400 jpg

Serial dilutions (1:2) of mouse IgG were resolved by PAGE, transferred onto nitrocellulose membrane and detected with the DuoLuX™ Substrate using either the Alkaline Phosphatase-based VECTASTAIN® ABC-AmP Kit (lower left) or Peroxidase Streptavidin (lower right). Graph shows prolonged light emission characteristics of the DuoLuX™ Substrate with each enzyme.

Fluorescent Properties

In addition to its chemiluminescent properties, the reaction product of the DuoLuX Substrate is also fluorescent. Fluorescence can be recorded with a digital imaging system or a conventional camera months after chemiluminescence has faded. The excitation maximum is at 405 nm, but other wavelengths (254 nm and 365 nm) also excite. Maximum fluorescent emission occurs at 453 nm. For fluorescence detection, nitrocellulose is recommended due to the intrinsic fluorescence of nylon. Acquisition of fluorescent signal requires a much shorter exposure time than chemiluminescence, often a fraction of a second.

DLfluor400 jpg

Western blot visualized by fluorescence using the VECTASTAIN® ABC-AmP Kit with the DuoLuX™ Substrate. Serial dilutions (1:2) of rabbit IgG heavy chain were resolved by PAGE, transferred onto nitrocellulose membrane, and detected with biotinylated anti-rabbit IgG, followed by the VECTASTAIN® ABC-AmP Kit with the DuoLuX™Substrate. [A] Fluorescent detection by image acquisition (1 second). [B] Fluorescent image of the same blot as shown in [A] obtained 12 months later. (Same 1 second acquisition time.)

Applications

The DuoLuX Chemiluminescent/Chemifluorescent Substrate is ideal for development of protein and nucleic acid blots.  Complete kits are available for western blot development (VECTASTAIN® ABC-AmP system).

Protein Detection

For western For western blot applications using chemiluminescent detection, the sensitivity using either alkaline phosphatase or peroxidase is approximately 1 pg of protein. Film exposure times using peroxidase are generally 5-30 seconds; exposure times for alkaline phosphatase are 1-5 minutes. Using fluorescence detection, sensitivities are less than 10 pg of protein. For western blots, DuoLuX™ Chemiluminescent/Chemifluorescent Substrate can be used on either nitrocellulose or PVDF membranes but, in general, nitrocellulose is preferred.

DLwestchemi400 jpg

Western blot visualized by chemiluminescence. Serial dilutions of rabbit IgG heavy chain were resolved by electrophoresis, transferred onto nitrocellulose membrane and detected with biotinylated anti-rabbit IgG, using the VECTASTAIN® ABC-AmP Kit with the DuoLuX™ Substrate. Chemiluminescent detection was captured by exposure to film.

DLwestfluor400 jpg

Western blot visualized by fluorescence. Serial dilutions (1:2) of mouse IgG were resolved by PAGE, transferred onto nitrocellulose membrane, and detected with biotinylated anti-mouse IgG, Peroxidase Streptavidin and the DuoLuX™ Substrate. Fluorescent image was obtained in 0.07 seconds.

Nucleic acid detection

For developing Southern, northern, or dot blots using alkaline phosphatase, typical film exposure times range from 30 seconds to 10 minutes with sensitivity ranging from 100 fg to 10 pg of nucleic acid. Using peroxidase, exposure times are somewhat shorter and sensitivities are slightly less than those for alkaline phosphatase. Both nitrocellulose or nylon membranes can be used.

DLsouthernchemi400 jpg

Southern blot of the E. coli phosphoenolpyruvate carboxylase gene.  Various dilutions of a 1.3 kb PCR product of the E. coli ppc gene was labeled with PHOTOPROBE® (LA) Biotin and hybridized to immobilized Tsp509 I-digested E. coli genomic DNA.  Probe was detected with Alkaline Phophatase Streptavidin followed by DuoLuX™ Substrate visualized by chemiluminescence.

The post DuoLuX® Chemiluminescent and Fluorescent Substrate, Peroxidase (HRP) appeared first on VectorLabs.

]]> Streptavidin, Peroxidase, R.T.U. https://staging.vectorlabs.com/products/streptavidin-horseradish-peroxidase-ready-to-use/ Fri, 28 Apr 2023 21:28:38 +0000 https://staging.vectorlabs.com/?post_type=product&p=13073 Description

Horseradish Peroxidase Streptavidin (HRP Streptavidin) is produced by our own coupling procedure which preserves the high specific activity of the peroxidase. Using biotinylated primary or secondary intermediates and peroxidase-labeled streptavidin, antigens can be localized in histological sections, cytospin preparations, or smears.

Features:

  • Avidin and streptavidin reagents are powerful tools to detect or purify biotinylated proteins, nucleic acids, and other macromolecules
  • Horseradish Peroxidase Streptavidin is offered as a concentrate (1 mg/ml) or in a ready-to-use (R.T.U.), prediluted, stabilized solution in a dropper bottle
  • For horseradish peroxidase streptavidin (Sav-HRP) that is optimized for use in solid-phase assays such as ELISAs and blotting applications, see the following product:
    Streptavidin, Horseradish Peroxidase (Sav-HRP), Concentrate, for ELISAs and Blots

Specifications

Unit Size 100 ml
Applications Immunohistochemistry / Immunocytochemistry, In situ hybridization, Blotting Applications, Elispot, ELISAs
Recommended Usage For most applications the recommended incubation time is 30 minutes. Do not use sodium azide with this product since it is an inhibitor of horseradish peroxidase. FOR LABORATORY USE.
Recommended Storage 2-8 °C, DO NOT FREEZE
Concentration 1.0 µg/ml active conjugate
Detection Enzyme(s) Peroxidase
Conjugate Peroxidase
Format Ready-to-Use

The post Streptavidin, Peroxidase, R.T.U. appeared first on VectorLabs.

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Description

Horseradish Peroxidase Streptavidin (HRP Streptavidin) is produced by our own coupling procedure which preserves the high specific activity of the peroxidase. Using biotinylated primary or secondary intermediates and peroxidase-labeled streptavidin, antigens can be localized in histological sections, cytospin preparations, or smears.

Features:

  • Avidin and streptavidin reagents are powerful tools to detect or purify biotinylated proteins, nucleic acids, and other macromolecules
  • Horseradish Peroxidase Streptavidin is offered as a concentrate (1 mg/ml) or in a ready-to-use (R.T.U.), prediluted, stabilized solution in a dropper bottle
  • For horseradish peroxidase streptavidin (Sav-HRP) that is optimized for use in solid-phase assays such as ELISAs and blotting applications, see the following product:
    Streptavidin, Horseradish Peroxidase (Sav-HRP), Concentrate, for ELISAs and Blots

Specifications

Unit Size100 ml
ApplicationsImmunohistochemistry / Immunocytochemistry, In situ hybridization, Blotting Applications, Elispot, ELISAs
Recommended UsageFor most applications the recommended incubation time is 30 minutes. Do not use sodium azide with this product since it is an inhibitor of horseradish peroxidase. FOR LABORATORY USE.
Recommended Storage2-8 °C, DO NOT FREEZE
Concentration1.0 µg/ml active conjugate
Detection Enzyme(s)Peroxidase
ConjugatePeroxidase
FormatReady-to-Use

Documents

Citations

The post Streptavidin, Peroxidase, R.T.U. appeared first on VectorLabs.

]]> Streptavidin, Peroxidase, ELISA Grade (Concentrate, for ELISAs and Blots) https://staging.vectorlabs.com/products/streptavidin-hrp-concentrate-elisas/ Fri, 28 Apr 2023 21:15:47 +0000 https://staging.vectorlabs.com/?post_type=product&p=13047 Description

Features:

  • Avidin and streptavidin reagents are powerful tools to detect or purify biotinylated proteins, nucleic acids, and other macromolecules.
  • Optimized for use in solid phase assays such as ELISAs and blotting applications
  • Horseradish Peroxidase Streptavidin (HRP Streptavidin) is produced by our own coupling procedure which preserves the high specific activity of the peroxidase
  • For horseradish peroxidase streptavidin (Sav-HRP) that is optimized for use in tissue/cell staining applications, see the following product:

    Streptavidin, Horseradish Peroxidase (Sav-HRP), Concentrate, for IHC/ICC

Specifications

Unit Size 1 mg
Applications Blotting Applications, Elispot, ELISAs
Recommended Usage Do not use sodium azide with this product since it is an inhibitor of horseradish peroxidase. Recommended concentration range for use is 5-10 µg/ml. Note: This product is different from Cat. No. SA-5004 which has been optimized for use in immunohistochemical applications.
Solution 0.05 M HEPES, 0.1M Na2S04 pH 6.8, preservative
Recommended Storage 2-8 °C, DO NOT FREEZE
Concentration 1.0 mg/ml active conjugate
Detection Enzyme(s) Peroxidase
Conjugate Peroxidase
Format Concentrate

The post Streptavidin, Peroxidase, ELISA Grade (Concentrate, for ELISAs and Blots) appeared first on VectorLabs.

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Description

Features:

  • Avidin and streptavidin reagents are powerful tools to detect or purify biotinylated proteins, nucleic acids, and other macromolecules.
  • Optimized for use in solid phase assays such as ELISAs and blotting applications
  • Horseradish Peroxidase Streptavidin (HRP Streptavidin) is produced by our own coupling procedure which preserves the high specific activity of the peroxidase

  • For horseradish peroxidase streptavidin (Sav-HRP) that is optimized for use in tissue/cell staining applications, see the following product:

    Streptavidin, Horseradish Peroxidase (Sav-HRP), Concentrate, for IHC/ICC

Specifications

Unit Size1 mg
ApplicationsBlotting Applications, Elispot, ELISAs
Recommended UsageDo not use sodium azide with this product since it is an inhibitor of horseradish peroxidase. Recommended concentration range for use is 5-10 µg/ml. Note: This product is different from Cat. No. SA-5004 which has been optimized for use in immunohistochemical applications.
Solution0.05 M HEPES, 0.1M Na2S04 pH 6.8, preservative
Recommended Storage2-8 °C, DO NOT FREEZE
Concentration1.0 mg/ml active conjugate
Detection Enzyme(s)Peroxidase
ConjugatePeroxidase
FormatConcentrate

Documents

Citations

Technical Information

Avidin and streptavidin reagents are powerful tools to detect or purify biotinylated proteins, nucleic acids, and other macromolecules.

Vector Laboratories enzyme-conjugated avidin and streptavidin are produced with the highest specific activity enzymes in optimal ratios. Specific covalent linkages are chosen to provide stable, highly active conjugates.

This Horseradish peroxidase streptavidin preparation (HRP-Sav; SA-5014) is optimized for use in solid-phase assays such as ELISAs and blotting applications.

For horseradish peroxidase streptavidin (Sav-HRP) that is optimized for use in tissue/cell staining applications, see the following product:

Streptavidin, Horseradish Peroxidase (Sav-HRP), Concentrate, for IHC/ICC

The post Streptavidin, Peroxidase, ELISA Grade (Concentrate, for ELISAs and Blots) appeared first on VectorLabs.

]]> Streptavidin, Peroxidase (Concentrate, for IHC) https://staging.vectorlabs.com/products/streptavidin-hrp-concentrate-ihc/ Fri, 28 Apr 2023 21:02:19 +0000 https://staging.vectorlabs.com/?post_type=product&p=13015 Description

Horseradish Peroxidase Streptavidin (HRP Streptavidin) is produced by our own coupling procedure that preserves the high specific activity of the peroxidase.  Using biotinylated primary or secondary intermediates and peroxidase-labeled streptavidin, antigens can be localized in histological sections, cytospin preparations, or smears.

Features:

  • Avidin and streptavidin reagents are powerful tools to detect or purify biotinylated proteins, nucleic acids, and other macromolecules
  • Horseradish Peroxidase Streptavidin is offered as a concentrate (1 mg/ml) or in a ready-to-use (R.T.U.), prediluted, stabilized solution in a dropper bottle

 

For horseradish peroxidase streptavidin (Sav-HRP) that is optimized for use in solid-phase assays such as ELISAs and blotting applications, see the following product:

Streptavidin, Horseradish Peroxidase (Sav-HRP), Concentrate, for ELISAs and Blots

Specifications

Unit Size 1 mg
Applications Immunohistochemistry / Immunocytochemistry, In situ hybridization
Recommended Usage Molar ratio active conjugate: 1.5. Do not use sodium azide with this product since it is an inhibitor of horseradish peroxidase. The recommended concentration range for use is 2-5 µg/ml.
Solution 0.05 M HEPES, 0.1M Na2S04 pH 6.8, preservative
Recommended Storage 2-8 °C, DO NOT FREEZE
Concentration 1.0 mg/ml active conjugate
Detection Enzyme(s) Peroxidase
Conjugate Peroxidase
Format Concentrate

The post Streptavidin, Peroxidase (Concentrate, for IHC) appeared first on VectorLabs.

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Description

Horseradish Peroxidase Streptavidin (HRP Streptavidin) is produced by our own coupling procedure that preserves the high specific activity of the peroxidase.  Using biotinylated primary or secondary intermediates and peroxidase-labeled streptavidin, antigens can be localized in histological sections, cytospin preparations, or smears.

Features:

  • Avidin and streptavidin reagents are powerful tools to detect or purify biotinylated proteins, nucleic acids, and other macromolecules
  • Horseradish Peroxidase Streptavidin is offered as a concentrate (1 mg/ml) or in a ready-to-use (R.T.U.), prediluted, stabilized solution in a dropper bottle

For horseradish peroxidase streptavidin (Sav-HRP) that is optimized for use in solid-phase assays such as ELISAs and blotting applications, see the following product:

Streptavidin, Horseradish Peroxidase (Sav-HRP), Concentrate, for ELISAs and Blots

Specifications

Unit Size1 mg
ApplicationsImmunohistochemistry / Immunocytochemistry, In situ hybridization
Recommended UsageMolar ratio active conjugate: 1.5. Do not use sodium azide with this product since it is an inhibitor of horseradish peroxidase. The recommended concentration range for use is 2-5 µg/ml.
Solution0.05 M HEPES, 0.1M Na2S04 pH 6.8, preservative
Recommended Storage2-8 °C, DO NOT FREEZE
Concentration1.0 mg/ml active conjugate
Detection Enzyme(s)Peroxidase
ConjugatePeroxidase
FormatConcentrate

Documents

Citations

The post Streptavidin, Peroxidase (Concentrate, for IHC) appeared first on VectorLabs.

]]> Wheat Germ Agglutinin (WGA), Peroxidase https://staging.vectorlabs.com/products/peroxidase-conjugated-wheat-germ-agglutinin/ Fri, 28 Apr 2023 19:51:54 +0000 https://staging.vectorlabs.com/?post_type=product&p=12841 Description

Peroxidase conjugated Wheat Germ Agglutinin is specially prepared for neuronal transport studies. This product is supplied as 40 mg/ml of protein in a 2 mg package ready for injection. After tissue preparation, detection can be readily achieved with a peroxidase substrate.

Specifications

Unit Size 2 mg
Applications Immunohistochemistry / Immunocytochemistry, Neurobiology, Blotting Applications, ELISAs, Glycobiology
Conjugate Peroxidase
Sugar Specificity N-Acetylglucosamine
Safety Title WARNING: Cancer – www.P65Warnings.ca.gov

The post Wheat Germ Agglutinin (WGA), Peroxidase appeared first on VectorLabs.

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Description

Peroxidase conjugated Wheat Germ Agglutinin is specially prepared for neuronal transport studies. This product is supplied as 40 mg/ml of protein in a 2 mg package ready for injection. After tissue preparation, detection can be readily achieved with a peroxidase substrate.

Specifications

Unit Size2 mg
ApplicationsImmunohistochemistry / Immunocytochemistry, Neurobiology, Blotting Applications, ELISAs, Glycobiology
ConjugatePeroxidase
Sugar SpecificityN-Acetylglucosamine
Safety TitleWARNING: Cancer – www.P65Warnings.ca.gov

Documents

Citations

Technical Information

Wheat germ agglutinin (WGA) contains a group of closely related isolectins, with an isoelectric point about pH 9. The receptor sugar for WGA is N-acetylglucosamine, with preferential binding to dimers and trimers of this sugar. WGA can bind oligosaccharides containing terminal N-acetylglucosamine or chitobiose, structures which are common to many serum and membrane glycoproteins. Bacterial cell wall peptidoglycans, chitin, cartilage glycosaminoglycans, and glycolipids can also bind WGA. Native WGA has also been reported to interact with some glycoproteins via sialic acid residues (see succinylated WGA). This lectin is used for the purification of insulin receptors and for neuronal tracing.

WGA-HRP can be used for both anterograde and retrograde tracing. Our peroxidase-wheat germ agglutinin is specially prepared for neuronal transport studies. WGA-peroxidase features a high concentration of protein and is supplied in a 2 mg package at 40 mg/ml ready for injection. Unconjugated wheat germ agglutinin, also used in neuronal studies is available as 10 mg and 25 mg of salt-free, lyophilized lectin.

Inhibiting/Eluting Sugar: Chitin Hydrolysate or 500 mM N-acetylglucosamine with salt and/or acid elution generally required

The post Wheat Germ Agglutinin (WGA), Peroxidase appeared first on VectorLabs.

]]> Horse Anti-Goat IgG Antibody (H+L), Peroxidase https://staging.vectorlabs.com/products/peroxidase-horse-anti-goat-igg/ Thu, 27 Apr 2023 18:57:03 +0000 https://staging.vectorlabs.com/?post_type=product&p=12678 Description

Features:

  • Affinity-purified, ultrapure, high affinity antibody
  • Suitable for use with goat, sheep, and bovine IgG primary antibodies
  • Thoroughly adsorbed against serum and immunoglobulins from potentially interfering species
  • Unless otherwise specified, antibodies recognizes both heavy and light chains (H+L)
  • Cross-linked with the highest specific activity horseradish peroxidase
  • Supplied in solution

Specifications

Unit Size 1 mg
Applications Immunohistochemistry / Immunocytochemistry, In situ hybridization, Blotting Applications, Elispot, ELISAs
Concentration 1 mg active conjugate/ml
Recommended Storage 2-8 °C, DO NOT FREEZE
Solution 10 mM HEPES, 0.15 M NaCl, pH 7.5, preservative
Recommended Usage Important Note: The use of bovine products such as casein, serum, albumin or non-fat dry milk as blocking agents in ELISA or blot assays may produce a high background due to cross-reactivity of anti-goat IgG with bovine immunoglobulins that may be present. If this antibody is to be used in thse applications, blocking with Animal-Free blocker™ solution (Cat. No. SP-5030) is recommended. Recommended diluent for microtitration plate assays: 10 mM phosphate, 0.15 M NaCl, pH 7.5, 0.1% Tween 20. Working concentration of HRP-anti goat IgG is 0.5-5.0 µg/ml.
Target Species Goat
Conjugate Peroxidase
Host Species Horse
Format Concentrate
Safety Title WARNING: Cancer – www.P65Warnings.ca.gov

The post Horse Anti-Goat IgG Antibody (H+L), Peroxidase appeared first on VectorLabs.

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Description

Features:

  • Affinity-purified, ultrapure, high affinity antibody 
  • Suitable for use with goat, sheep, and bovine IgG primary antibodies
  • Thoroughly adsorbed against serum and immunoglobulins from potentially interfering species 
  • Unless otherwise specified, antibodies recognizes both heavy and light chains (H+L) 
  • Cross-linked with the highest specific activity horseradish peroxidase 
  • Supplied in solution

Specifications

Unit Size1 mg
ApplicationsImmunohistochemistry / Immunocytochemistry, In situ hybridization, Blotting Applications, Elispot, ELISAs
Concentration1 mg active conjugate/ml
Recommended Storage2-8 °C, DO NOT FREEZE
Solution10 mM HEPES, 0.15 M NaCl, pH 7.5, preservative
Recommended UsageImportant Note: The use of bovine products such as casein, serum, albumin or non-fat dry milk as blocking agents in ELISA or blot assays may produce a high background due to cross-reactivity of anti-goat IgG with bovine immunoglobulins that may be present. If this antibody is to be used in thse applications, blocking with Animal-Free blocker™ solution (Cat. No. SP-5030) is recommended. Recommended diluent for microtitration plate assays: 10 mM phosphate, 0.15 M NaCl, pH 7.5, 0.1% Tween 20. Working concentration of HRP-anti goat IgG is 0.5-5.0 µg/ml.
Target SpeciesGoat
ConjugatePeroxidase
Host SpeciesHorse
FormatConcentrate
Safety TitleWARNING: Cancer – www.P65Warnings.ca.gov

Documents

Citations

Technical Information

Our high affinity Horse Anti-Goat IgG Antibody is purified by affinity chromatography and adsorbed to remove any cross-reactivities that are likely to interfere with specific labeling. This antibody is then cross-linked with the highest specific activity horseradish peroxidase. A proprietary procedure for conjugation allows the maximum preservation of enzyme activity and antibody specificity. This antibody is subjected to rigorous quality control assays and can be used for tissue staining, ELISAs, and blots.

As well as recognizing goat IgG, this antibody is suitable to detect sheep and bovine IgG primary antibodies.

The use of bovine products such as casein, serum, albumin or non-fat dry milk as blocking agents in ELISA or blot assays may produce a high background due to cross-reactivity of this antibody with bovine immunoglobulins that may be present. If this antibody is to be used in these applications, blocking with Animal-Free Blocker® solution (Cat. No. SP-5030) is recommended.

The post Horse Anti-Goat IgG Antibody (H+L), Peroxidase appeared first on VectorLabs.

]]> Goat Anti-Rat IgG, Mouse Adsorbed, Antibody (H+L), Peroxidase https://staging.vectorlabs.com/products/peroxidase-goat-anti-rat-igg-mouse-adsorbed/ Thu, 27 Apr 2023 18:54:00 +0000 https://staging.vectorlabs.com/?post_type=product&p=12671 Description

HRP Goat Anti-Rat IgG, Mouse Adsorbed binds to rat primary antibodies for tissue staining, ELISAs, and blots. It is designed for use with mouse tissues that may contain endogenous mouse immunoglobulins.

Features:

  • Recognizes both heavy and light chains (H+L)
  • Cross-linked with the highest specific activity horseradish peroxidase
  • Supplied in solution

Specifications

Unit Size 0.5 mg
Applications Immunohistochemistry / Immunocytochemistry, Blotting Applications, Elispot, ELISAs
Concentration 1 mg active conjugate/ml
Recommended Storage 2-8 °C, DO NOT FREEZE
Solution 10 mM HEPES, 0.15 M NaCl, pH 7.5, preservative
Recommended Usage Recommended diluent for microtitration plate assays: 10 mM phosphate, 0.15 M NaCl, pH 7.5, 0.1% Tween 20. Working concentration of HRP-anti rat IgG is 0.5-5.0 µg/ml.
Target Species Rat
Conjugate Peroxidase
Host Species Goat
Format Concentrate
Safety Title WARNING: Cancer – www.P65Warnings.ca.gov

The post Goat Anti-Rat IgG, Mouse Adsorbed, Antibody (H+L), Peroxidase appeared first on VectorLabs.

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Description

HRP Goat Anti-Rat IgG, Mouse Adsorbed binds to rat primary antibodies for tissue staining, ELISAs, and blots. It is designed for use with mouse tissues that may contain endogenous mouse immunoglobulins.

Features:

  • Recognizes both heavy and light chains (H+L) 
  • Cross-linked with the highest specific activity horseradish peroxidase 
  • Supplied in solution

Specifications

Unit Size0.5 mg
ApplicationsImmunohistochemistry / Immunocytochemistry, Blotting Applications, Elispot, ELISAs
Concentration1 mg active conjugate/ml
Recommended Storage2-8 °C, DO NOT FREEZE
Solution10 mM HEPES, 0.15 M NaCl, pH 7.5, preservative
Recommended UsageRecommended diluent for microtitration plate assays: 10 mM phosphate, 0.15 M NaCl, pH 7.5, 0.1% Tween 20. Working concentration of HRP-anti rat IgG is 0.5-5.0 µg/ml.
Target SpeciesRat
ConjugatePeroxidase
Host SpeciesGoat
FormatConcentrate
Safety TitleWARNING: Cancer – www.P65Warnings.ca.gov

Documents

Citations

Technical Information

Our high affinity Goat Anti-Rat IgG Antibody is purified by affinity chromatography and adsorbed to remove any cross-reactivities that are likely to interfere with specific labeling. This antibody is then cross-linked with the highest specific activity horseradish peroxidase. A proprietary procedure for conjugation allows the maximum preservation of enzyme activity and antibody specificity. 

This specially prepared antibody is designed to be used on tissues that may contain interfering endogenous immunoglobulins. The anti-rat IgG (H+L), mouse adsorbed products have been thoroughly adsorbed by a solid phase technique to remove antibodies that cross-react with mouse immunoglobulins. This adsorbed antibody allows primary antibodies made in rat to be used on mouse tissues.

Peroxidase Goat Anti-Rat IgG, mouse adsorbed, is supplied in solution. With some exceptions, working concentration of HRP Anti-Rat IgG, mouse adsorbed, is 0.5-5.0 μg/ml.

The post Goat Anti-Rat IgG, Mouse Adsorbed, Antibody (H+L), Peroxidase appeared first on VectorLabs.

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