Description

Hydroxy-dPEG®24-TFP ester, product number QBD-11349, is a long (76 atoms, 88.8 Å), hydroxy-terminated, single molecular weight, discrete polyethylene glycol (dPEG®) compound functionalized with a 2,3,5,6-tetrafluorophenyl (TFP) ester for conjugation to primary and secondary amines. This product can be used for modification of amine-functionalized surfaces (e.g., silica, glass), modification of proteins and peptides at accessible amine sites on the molecules, the construction of discrete PEG dendrimers, and many other applications. The free hydroxy end of the dPEG® spacer can be functionalized for further reactions or left unmodified to increase hydrophilicity or modify the charge of conjugate molecules and surfaces.

Specifications

Documents

References

Greg T. Hermanson, Bioconjugate Techniques, 2nd Edition, Elsevier Inc., Burlington, MA 01803, April, 2008 (ISBN-13: 978-0-12-370501-3; ISBN-10: 0-12-370501-0). Specifically see pp. 726-729 in his Chapter 18 on discrete PEG compounds for pegylation applications.

Greg T. Hermanson, Bioconjugate Techniques, 3rd Edition, Elsevier, Waltham, MA 02451, 2013, ISBN 978-0-12-382239-0; See chapter 18, Discrete PEG Reagents, pp.787-821, for a full overview of the dPEG® products.

Description

Hydroxy-dPEG®12-TFP ester, product number QBD-11345, is a medium-length (40 atoms, 46.2 Å), hydroxy-terminated, single molecular weight, discrete polyethylene glycol (dPEG®) compound functionalized with a 2,3,5,6-tetrafluorophenyl (TFP) ester for conjugation to primary and secondary amines. This product can be used for modification of amine-functionalized surfaces (e.g., silica, glass), modification of proteins and peptides at accessible amine sites on the molecules, the construction of discrete PEG dendrimers, and many other applications. The free hydroxy end of the dPEG® spacer can be functionalized for further reactions or left unmodified to increase hydrophilicity or modify the charge of conjugate molecules and surfaces.

Specifications

Documents

References

Greg T. Hermanson, Bioconjugate Techniques, 2nd Edition, Elsevier Inc., Burlington, MA 01803, April, 2008 (ISBN-13: 978-0-12-370501-3; ISBN-10: 0-12-370501-0). Specifically see pp. 726-729 in his Chapter 18 on discrete PEG compounds for pegylation applications.

Greg T. Hermanson, Bioconjugate Techniques, 3rd Edition, Elsevier, Waltham, MA 02451, 2013, ISBN 978-0-12-382239-0; See chapter 18, Discrete PEG Reagents, pp.787-821, for a full overview of the dPEG® products.

Description

Hydroxy-dPEG®4-TFP ester, product number QBD-11341, is a short (16 atoms, 17.9 Å), hydroxy-terminated, single molecular weight, discrete polyethylene glycol (dPEG®) compound functionalized with a 2,3,5,6-tetrafluorophenyl (TFP) ester for conjugation to primary and secondary amines. This product can be used for modification of amine-functionalized surfaces (e.g., silica, glass), modification of proteins and peptides at accessible amine sites on the molecules, the construction of discrete PEG dendrimers, and many other applications. The free hydroxy end of the dPEG® spacer can be functionalized for further reactions or left unmodified to increase hydrophilicity or modify the charge of conjugate molecules and surfaces.

Specifications

Documents

References

Greg T. Hermanson, Bioconjugate Techniques, 2nd Edition, Elsevier Inc., Burlington, MA 01803, April, 2008 (ISBN-13: 978-0-12-370501-3; ISBN-10: 0-12-370501-0). Specifically see pp. 726-729 in his Chapter 18 on discrete PEG compounds for pegylation applications.

Greg T. Hermanson, Bioconjugate Techniques, 3rd Edition, Elsevier, Waltham, MA 02451, 2013, ISBN 978-0-12-382239-0; See chapter 18, Discrete PEG Reagents, pp.787-821, for a full overview of the dPEG® products.

Description

S-acetyl-dPEG®12-OH, product number QBD-10939, contains an acetyl-protected thiol on a medium-length (36 atoms, 43.1 Å) hydroxy-terminated, discrete polyethylene glycol (dPEG®) linker. This product can be used to thiolate biomolecules.

Bioconjugation frequently employs thiolation because the reactions to install thiol groups on molecules or to react with molecules containing sulfhydryl groups are relatively simple and often chemoselective. The thioacetyl group is intended to install an acetyl-protected thiol onto biomolecules. While installing a protected thiol through a reaction between a carboxylic acid and a primary amine is widely popular in bioconjugation technology, it is not always possible or feasible. S-acetyl-dPEG®12-OH permits the installation of a protected thiol through a short, hydroxy-terminated discrete PEG® linker. Hydroxylamine hydrochloride (CAS number 5470-11-1) easily removes the S-acetyl group that protects the thiol, thus exposing the sulfhydryl for subsequent reaction. One application for S-acetyl-dPEG®12-OH is the modification of nucleic acids using phosphoramidite chemistry.

Specifications

Documents

References

Greg T. Hermanson, Bioconjugate Techniques, 3rd Edition, Elsevier, Waltham, MA 02451, 2013, ISBN 978-0-12-382239-0; See Chapter 18, Discrete PEG Reagents, pp. 787-821, for a full overview of the dPEG® products.

Description

Amino-dPEG®36-OH, product number QBD-10869, also known as Amino-dPEG®36-alcohol, is a long (107 atoms, 129.0 Å), monodispersed PEGylation reagent designed to modify surfaces and biomolecules. One end of the molecule terminates with a primary amine, while the other end terminates with a primary alcohol group. This extremely hydrophilic PEGylation reagent reacts with carboxylate groups via the primary amine, leaving the hydroxy terminus free for functionalization and further reactions or as a hydrophilicity or charge modifier for the conjugate molecule.

The most common uses for Amino-dPEG®36-OH include modifying biomolecules and passivating surfaces with a highly hydrophilic coating. The primary amine on one end of the dPEG® linker reacts with carboxylates to form stable amide bonds and with aldehydes and ketones to form labile Schiff bases that can be reduced to secondary amines under mild conditions for greater stability. Conjugations with this molecule most commonly use the amine-carboxylate reaction with a carbodiimide such as EDC. Alternatively, activation of carboxylate as the NHS or TFP ester followed by reaction under slightly basic conditions also permits conjugation of the carboxylate and amino moieties.

Specifications

Documents

References

Greg T. Hermanson, Bioconjugate Techniques, 3rd Edition, Elsevier, Waltham, MA 02451, 2013, ISBN 978-0-12-382239-0; See Chapter 18, Discrete PEG Reagents, pp. 787-821, for a full overview of the dPEG® products.

Description

Azido-dPEG®36-OH (Azido-dPEG®36-alcohol), product number QBD-10544, is a click chemistry reagent used to modify surfaces and biomolecules or to crosslink with other surfaces or biomolecules in supramolecular construction. This product consists of an azide and a terminal hydroxy group separated by a long (108 atoms), single molecular weight, discrete-length polyethylene glycol (dPEG®) chain. The terminal azide reacts in copper(I)-catalyzed, ruthenium-catalyzed, and strain-promoted azide-alkyne cycloaddition reactions (CuAAC, RuAAC, and SPAAC, respectively). If left unmodified, the alcohol group increases the water solubility and hydrodynamic volume of molecules and surfaces to which it is conjugated. Modifying the alcohol with a reactive group such as chloride, tosylate, or mesylate allows Azido-dPEG®36-OH to function as a crosslinker through subsequent chemical manipulations.

As a click chemistry reagent, azido-dPEG®36-OH can participate in CuAAC, RuAAC, and SPAAC reactions. Because Vector Laboratories’ dPEG® products are amphiphilic, these reactions can occur almost equally well in water or organic solvents. The primary alcohol group on the opposite end of the linker can be used as a neutrally charged functional group. In this function, the alcohol enhances the water solubility of the conjugate molecule. Alternatively, the alcohol group can be functionalized with reactive groups and used to modify the conjugate further. Thus, azido-dPEG®36-OH can be used in conjugations with biomolecules where organic solvents may damage the conjugate target. This product was published in a 2014 paper demonstrating high-throughput modifications of siRNA via CuAAC.

Specifications

Documents

References

Greg T. Hermanson, Bioconjugate Techniques, 3rd Edition, Elsevier, Waltham, MA 02451, 2013, ISBN 978-0-12-382239-0; See Chapter 18, Discrete PEG Reagents, pp. 787-821, for a full overview of the dPEG® products.

Application of Multivalent Interactions for Recognition Imaging and Delivery of Therapeutics. Saikat Manna. August 2016.

Description

Azido-dPEG®24-OH (Azido-dPEG®24-alcohol), product number QBD-10543, is a click chemistry reagent used to modify surfaces and biomolecules or to crosslink with other surfaces or biomolecules in supramolecular construction. This product consists of an azide and a terminal hydroxy group separated by a long (72 atoms), single molecular weight, discrete-length polyethylene glycol (dPEG®) chain. The terminal azide reacts in copper(I)-catalyzed, ruthenium-catalyzed, and strain-promoted azide-alkyne cycloaddition reactions (CuAAC, RuAAC, and SPAAC, respectively). If left unmodified, the alcohol group increases the water solubility and hydrodynamic volume of molecules and surfaces to which it is conjugated. Modifying the alcohol with a reactive group such as chloride, tosylate, or mesylate allows Azido-dPEG®24-OH to function as a crosslinker through subsequent chemical manipulations.

As a click chemistry reagent, azido-dPEG®24-OH can participate in CuAAC, RuAAC, and SPAAC reactions. Because Vector Laboratories’ dPEG® products are amphiphilic, these reactions can occur almost equally well in water or organic solvents. Thus, azido-dPEG®24-OH can be used in conjugations with biomolecules where organic solvents may damage the conjugate target.

The primary alcohol group on the opposite end of the linker can be used as a neutrally charged functional group. In this function, the alcohol enhances the water solubility of the conjugate molecule. Alternatively, the alcohol group can be functionalized with reactive groups and used to modify the conjugate further.

Specifications

Documents

References

Greg T. Hermanson, Bioconjugate Techniques, 3rd Edition, Elsevier, Waltham, MA 02451, 2013, ISBN 978-0-12-382239-0; See Chapter 18, Discrete PEG Reagents, pp. 787-821, for a full overview of the dPEG® products.

High-Throughput Chemical Modification of Oligonucleotides for Systematic Structure-Activity Relationship Evaluation. Daniel Zewge, Francis Gosselin, David Matthew Tellers, Ian W. Davies, Vasant Jadhav, Sandhya S. Nerurkar, Yu Yuan, Jenny Li, W. Michael Flanagan, and Denise M. Kenski. Bioconjugate Chemistry. 2014, November 14, 2014. DOI” 10.1021/bc500453q.

Description

Azido-dPEG®8-OH (Azido-dPEG®8-alcohol), product number QBD-10542, is a click chemistry reagent used to modify surfaces and biomolecules or to crosslink with other surfaces or biomolecules in supramolecular construction. This product consists of an azide and a terminal hydroxy group separated by a medium-length (24 atoms), single molecular weight, discrete-length polyethylene glycol (dPEG®) chain. The terminal azide reacts in copper(I)-catalyzed, ruthenium-catalyzed, and strain-promoted azide-alkyne cycloaddition reactions (CuAAC, RuAAC, and SPAAC, respectively). If left unmodified, the alcohol group increases the water solubility and hydrodynamic volume of molecules and surfaces to which it is conjugated. Modifying the alcohol with a reactive group such as chloride, tosylate, or mesylate allows Azido-dPEG®8-OH to function as a crosslinker through subsequent chemical manipulations.

As a click chemistry reagent, azido-dPEG®8-OH can participate in CuAAC, RuAAC, and SPAAC reactions. Because Vector Laboratories’ dPEG® products are amphiphilic, these reactions can occur almost equally well in water or organic solvents. Thus, azido-dPEG®8-OH can be used in conjugations with biomolecules where organic solvents may damage the conjugate target.

The primary alcohol group on the opposite end of the linker can be used as a neutrally charged functional group. In this function, the alcohol enhances the water solubility of the conjugate molecule. Alternatively, the alcohol group can be functionalized with reactive groups and used to modify the conjugate further.

Specifications

Documents

References

Greg T. Hermanson, Bioconjugate Techniques, 3rd Edition, Elsevier, Waltham, MA 02451, 2013, ISBN 978-0-12-382239-0; See Chapter 18, Discrete PEG Reagents, pp. 787-821, for a full overview of the dPEG® products.

Description

Azido-dPEG®4-OH (Azido-dPEG®4-alcohol), product number QBD-10541, is a click chemistry reagent used to modify surfaces and biomolecules or to crosslink with other surfaces or biomolecules in supramolecular construction. This product consists of an azide and a terminal hydroxy group separated by a short (14 atoms), single molecular weight, discrete-length polyethylene glycol (dPEG®) chain. The terminal azide reacts in copper(I)-catalyzed, ruthenium-catalyzed, and strain-promoted azide-alkyne cycloaddition reactions (CuAAC, RuAAC, and SPAAC, respectively). If left unmodified, the alcohol group increases the water solubility and hydrodynamic volume of molecules and surfaces to which it is conjugated. Modifying the alcohol with a reactive group such as chloride, tosylate, or mesylate allows Azido-dPEG®4-OH to function as a crosslinker through subsequent chemical manipulations.

As a click chemistry reagent, azido-dPEG®4-OH can participate in CuAAC, RuAAC, and SPAAC reactions. Because Vector Laboratories’ dPEG® products are amphiphilic, these reactions can occur almost equally well in water or organic solvents. Thus, azido-dPEG®4-OH can be used in conjugations with biomolecules where organic solvents may damage the conjugate target.

The primary alcohol group on the opposite end of the linker can be used as a neutrally charged functional group. In this function, the alcohol enhances the water solubility of the conjugate molecule. Alternatively, the alcohol group can be functionalized with reactive groups and used to modify the conjugate further.

Specifications

Documents

References

Greg T. Hermanson, Bioconjugate Techniques, 3rd Edition, Elsevier, Waltham, MA 02451, 2013, ISBN 978-0-12-382239-0; See Chapter 18, Discrete PEG Reagents, pp. 787-821, for a full overview of the dPEG® products.

Description

Azido-dPEG®12-OH (Azido-dPEG®12-alcohol), product number QBD-10340, is a click chemistry reagent used to modify surfaces and biomolecules or to crosslink with other surfaces or biomolecules in supramolecular construction. This product consists of an azide and a terminal hydroxy group separated by a medium-length (37 atoms), single molecular weight, discrete-length polyethylene glycol (dPEG®) chain. The terminal azide reacts in copper(I)-catalyzed, ruthenium-catalyzed, and strain-promoted azide-alkyne cycloaddition reactions (CuAAC, RuAAC, and SPAAC, respectively). If left unmodified, the alcohol group increases the water solubility and hydrodynamic volume of molecules and surfaces to which it is conjugated. Modifying the alcohol with a reactive group such as chloride, tosylate, or mesylate allows Azido-dPEG®12-OH to function as a crosslinker through subsequent chemical manipulations.

As a click chemistry reagent, azido-dPEG®12-OH can participate in CuAAC, RuAAC, and SPAAC reactions. Because Vector Laboratories’ dPEG® products are amphiphilic, these reactions can occur almost equally well in water or organic solvents. Thus, azido-dPEG®12-OH can be used in conjugations with biomolecules where organic solvents may damage the conjugate target.

The primary alcohol group on the opposite end of the linker can be used as a neutrally charged functional group. In this function, the alcohol enhances the water solubility of the conjugate molecule. Alternatively, the alcohol group can be functionalized with reactive groups and used to modify the conjugate further.

Specifications

Documents

References

Greg T. Hermanson, Bioconjugate Techniques, 3rd Edition, Elsevier, Waltham, MA 02451, 2013, ISBN 978-0-12-382239-0; See Chapter 18, Discrete PEG Reagents, pp. 787-821, for a full overview of the dPEG® products.