Mitogenic Stimulation – VectorLabs https://staging.vectorlabs.com From linker design and synthesis, bioconjugated design and manufacturing, biomolecule labeling and functionalization, and detection system and solutions, our team is here to help you move forward, with impact. Wed, 22 Oct 2025 17:14:51 +0000 en-US hourly 1 https://wordpress.org/?v=6.9 https://staging.vectorlabs.com/wp-content/uploads/2024/11/V-favicon-02-100x100.png Mitogenic Stimulation – VectorLabs https://staging.vectorlabs.com 32 32 Jacalin, Unconjugated https://staging.vectorlabs.com/products/unconjugated-jacalin/ Tue, 02 May 2023 14:44:44 +0000 https://staging.vectorlabs.com/?post_type=product&p=14147 Description

This lectin appears to bind only O-glycosidically linked oligosaccharides, preferring the structure galactosyl (β-1,3) N-acetylgalactosamine. This structure (the T-antigen) is the oligosaccharide to which peanut agglutinin (PNA) binds. However, unlike PNA, Jacalin will bind a mono- or disialylated form of this structure. This lectin has been used to purify human IgA. The specificity of this lectin also affords the opportunity to localize or isolate glycoproteins with O-glycosidically linked oligosaccharide side chains.

Specifications

Unit Size 25 mg
Applications Glycobiology, Mitogenic Stimulation
Recommended Usage Although many buffers can be employed for reconstituting and diluting this lectin, 10 mM HEPES buffered saline, pH 8.5, 0.1 mM CaCl2 is recommended.For preserving solutions, stored at 4 ºC, 0.08% sodium azide can be used.
Recommended Storage 2-8 °C
Conjugate Unconjugated
Sugar Specificity Galactose

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Description

This lectin appears to bind only O-glycosidically linked oligosaccharides, preferring the structure galactosyl (β-1,3) N-acetylgalactosamine. This structure (the T-antigen) is the oligosaccharide to which peanut agglutinin (PNA) binds. However, unlike PNA, Jacalin will bind a mono- or disialylated form of this structure. This lectin has been used to purify human IgA. The specificity of this lectin also affords the opportunity to localize or isolate glycoproteins with O-glycosidically linked oligosaccharide side chains.

Specifications

Unit Size25 mg
ApplicationsGlycobiology, Mitogenic Stimulation
Recommended UsageAlthough many buffers can be employed for reconstituting and diluting this lectin, 10 mM HEPES buffered saline, pH 8.5, 0.1 mM CaCl2 is recommended.For preserving solutions, stored at 4 ºC, 0.08% sodium azide can be used.
Recommended Storage2-8 °C
ConjugateUnconjugated
Sugar SpecificityGalactose

Documents

Citations

Technical Information

Jacalin is a lectin composed of four subunits of approximately 16 kDa each. This lectin appears to bind only O-glycosidically linked oligosaccharides, preferring the structure galactosyl (β-1,3) N-acetylgalactosamine. This structure (the T-antigen) is the oligosaccharide to which peanut agglutinin (PNA) binds. However, unlike PNA, Jacalin will bind a mono- or disialylated form of this structure. This lectin has been used to purify human IgA. The specificity of this lectin also affords the opportunity to localize or isolate glycoproteins with O-glycosidically linked oligosaccharide side chains.

Inhibiting/Eluting Sugar: 800 mM galactose or 100 mM melibiose

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]]> Lens Culinaris Agglutinin (LCA), Unconjugated https://staging.vectorlabs.com/products/unconjugated-lens-culinaris-agglutinin/ Tue, 02 May 2023 14:31:38 +0000 https://staging.vectorlabs.com/?post_type=product&p=14116 Description

LCA recognizes sequences containing α-linked mannose residues but recognizes additional sugars as part of the receptor structure, giving it a narrower specificity than Con A. An α-linked fucose residue attached to the N-acetylchitobiose portion of the core oligosaccharide significantly enhances affinity. By exploiting this narrower specificity, glycoproteins and glycopeptides can be subfractionated with LCA after initial isolation with Con A.

Specifications

 
Unit Size 10 mg, 25 mg
Applications Immunohistochemistry / Immunocytochemistry, Immunofluorescence, Blotting Applications, Glycobiology, Mitogenic Stimulation
Recommended Usage Although many buffers can be employed for reconstituting and diluting this lectin, 10 mM HEPES buffered saline, pH 8.5, 0.1 mM CaCl2 is recommended. For preserving solutions stored at 4 ºC, 0.08% sodium azide can be used.
Recommended Storage 2-8 °C
Inhibiting and/or Eluting Sugar mixture of 200 mM α−methylmannoside/200 mM α−methylglucoside
Conjugate Unconjugated
Sugar Specificity Mannose, Glucose

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Description

LCA recognizes sequences containing α-linked mannose residues but recognizes additional sugars as part of the receptor structure, giving it a narrower specificity than Con A. An α-linked fucose residue attached to the N-acetylchitobiose portion of the core oligosaccharide significantly enhances affinity. By exploiting this narrower specificity, glycoproteins and glycopeptides can be subfractionated with LCA after initial isolation with Con A.

Specifications

 
Unit Size10 mg, 25 mg
ApplicationsImmunohistochemistry / Immunocytochemistry, Immunofluorescence, Blotting Applications, Glycobiology, Mitogenic Stimulation
Recommended UsageAlthough many buffers can be employed for reconstituting and diluting this lectin, 10 mM HEPES buffered saline, pH 8.5, 0.1 mM CaCl2 is recommended. For preserving solutions stored at 4 ºC, 0.08% sodium azide can be used.
Recommended Storage2-8 °C
Inhibiting and/or Eluting Sugarmixture of 200 mM α−methylmannoside/200 mM α−methylglucoside
ConjugateUnconjugated
Sugar SpecificityMannose, Glucose

Documents

Citations

Technical Information

Lens culinaris agglutinin is composed of four subunits – two of about 17 kDa and two of 8 kDa. LCA has been found to be one of the most effective agents in preventing skin allograft rejection in model systems. LCA has been used to purify numerous glycoproteins, including immunoglobulins, histocompatibility antigens, and α2-macroglobulin.

Inhibiting/Eluting Sugar: mixture of 200 mM α-methylmannoside/200 mM α-methylglucoside

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]]> Wheat Germ Agglutinin (WGA), Unconjugated https://staging.vectorlabs.com/products/unconjugated-wheat-germ-agglutinin/ Tue, 02 May 2023 14:24:22 +0000 https://staging.vectorlabs.com/?post_type=product&p=14093

Description

The receptor sugar for WGA is N-acetylglucosamine, with preferential binding to dimers and trimers of this sugar. WGA can bind oligosaccharides containing terminal N-acetylglucosamine or chitobiose, structures which are common to many serum and membrane glycoproteins. Bacterial cell wall peptidoglycans, chitin, cartilage glycosaminoglycans, and glycolipids can also bind WGA. Native WGA has also been reported to interact with some glycoproteins via sialic acid residues (see succinylated WGA).

Specifications

Unit Size 10 mg, 25 mg
Applications Immunohistochemistry / Immunocytochemistry, Immunofluorescence, Blotting Applications, Glycobiology, Mitogenic Stimulation
Recommended Usage Although many buffers can be employed for reconstituting and diluting this lectin, 10 mM HEPES buffered saline, pH 8.5, 0.1 mM CaCl2 is recommended. For preserving solutions stored at 4 ºC, 0.08% sodium azide can be used.
Recommended Storage 2-8 °C
Conjugate Unconjugated

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Description

The receptor sugar for WGA is N-acetylglucosamine, with preferential binding to dimers and trimers of this sugar. WGA can bind oligosaccharides containing terminal N-acetylglucosamine or chitobiose, structures which are common to many serum and membrane glycoproteins. Bacterial cell wall peptidoglycans, chitin, cartilage glycosaminoglycans, and glycolipids can also bind WGA. Native WGA has also been reported to interact with some glycoproteins via sialic acid residues (see succinylated WGA).

Specifications

Unit Size10 mg, 25 mg
ApplicationsImmunohistochemistry / Immunocytochemistry, Immunofluorescence, Blotting Applications, Glycobiology, Mitogenic Stimulation
Recommended UsageAlthough many buffers can be employed for reconstituting and diluting this lectin, 10 mM HEPES buffered saline, pH 8.5, 0.1 mM CaCl2 is recommended. For preserving solutions stored at 4 ºC, 0.08% sodium azide can be used.
Recommended Storage2-8 °C
ConjugateUnconjugated
Sugar SpecificityN-Acetylglucosamine

Documents

Citations

Technical Information

Wheat germ agglutinin (WGA) contains a group of closely related isolectins, with an isoelectric point about pH 9. This lectin is used for the purification of insulin receptors and for neuronal tracing.

Inhibiting/Eluting Sugar: Chitin Hydrolysate or 500 mM N-acetylglucosamine with salt and/or acid elution generally required

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]]> Soybean Agglutinin (SBA), Unconjugated https://staging.vectorlabs.com/products/unconjugated-soybean-agglutinin/ Tue, 02 May 2023 14:19:57 +0000 https://staging.vectorlabs.com/?post_type=product&p=14082 Description

SBA preferentially binds to oligosaccharide structures with terminal α- or β-linked N-acetylgalactosamine, and to a lesser extent, galactose residues. Binding can be blocked by substitutions on penultimate sugars, such as fucose attached to the penultimate galactose in blood group B substance.

Specifications

More Information
Unit Size 10 mg, 25 mg
Applications Immunohistochemistry / Immunocytochemistry, Immunofluorescence, Blotting Applications, Glycobiology, Mitogenic Stimulation
Recommended Usage Although many buffers can be employed for reconstituting and diluting this lectin, we recommend 10 mM HEPES buffered saline, pH 8.5, 0.1 mM CaCl2. For preserving solutions stored at 4 ºC, 0.08% sodium azide can be used.
Recommended Storage 2-8 °C
Conjugate Unconjugated
Sugar Specificity Galactose, N-Acetylgalactosamine

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Description

SBA preferentially binds to oligosaccharide structures with terminal α- or β-linked N-acetylgalactosamine, and to a lesser extent, galactose residues. Binding can be blocked by substitutions on penultimate sugars, such as fucose attached to the penultimate galactose in blood group B substance.

Specifications

More Information
Unit Size10 mg, 25 mg
ApplicationsImmunohistochemistry / Immunocytochemistry, Immunofluorescence, Blotting Applications, Glycobiology, Mitogenic Stimulation
Recommended UsageAlthough many buffers can be employed for reconstituting and diluting this lectin, we recommend 10 mM HEPES buffered saline, pH 8.5, 0.1 mM CaCl2. For preserving solutions stored at 4 ºC, 0.08% sodium azide can be used.
Recommended Storage2-8 °C
ConjugateUnconjugated
Sugar SpecificityGalactose, N-Acetylgalactosamine

Documents

Citations

Technical Information

Composed of four subunits of approximately equal size, soybean agglutinin is a family of closely related isolectins. This glycoprotein has a molecular weight of about 120 kDa and an isoelectric point near pH 6.0. SBA preferentially binds to oligosaccharide structures with terminal α- or β-linked N-acetylgalactosamine, and to a lesser extent, galactose residues. Binding can be blocked by substitutions on penultimate sugars, such as fucose attached to the penultimate galactose in blood group B substance.

An important application for SBA is the separation of pluripotent stem cells from human bone marrow. Cells fractionated by SBA do not produce graft vs host disease and can be used in bone marrow transplantation across histocompatibility barriers.

Inhibiting/Eluting Sugar: 200 mM N-acetylgalactosamine

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]]> Wisteria Floribunda Lectin (WFA, WFL), Unconjugated https://staging.vectorlabs.com/products/unconj-wisteria-floribunda-lectin-wfa-wfl/ Thu, 27 Apr 2023 17:22:30 +0000 https://staging.vectorlabs.com/?post_type=product&p=12504 Description

The binding specificity of Wisteria floribunda lectin (WFL) is not completely clear but this lectin appears to preferentially bind carbohydrate structures terminating in N-acetylgalactosamine linked α or β to the 3 or 6 position of galactose. This lectin has been used to fractionate lymphocyte populations, and although not mitogenic, elicits the production of lymphokines from murine splenocytes.

Specifications

Unit Size 5 mg
Applications Glycobiology, Mitogenic Stimulation
Recommended Usage Although many buffers can be employed for reconstituting and diluting this lectin, 10 mM HEPES buffered saline, pH 8.5, 0.1 mM CaCl2 is recommended. For preserving solutions stored at 4 ºC, 0.08% sodium azide can be used.
Recommended Storage 2-8 °C
Conjugate Unconjugated
Sugar Specificity N-Acetylgalactosamine

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Description

The binding specificity of Wisteria floribunda lectin (WFL) is not completely clear but this lectin appears to preferentially bind carbohydrate structures terminating in N-acetylgalactosamine linked α or β to the 3 or 6 position of galactose. This lectin has been used to fractionate lymphocyte populations, and although not mitogenic, elicits the production of lymphokines from murine splenocytes.

Specifications

Unit Size5 mg
ApplicationsGlycobiology, Mitogenic Stimulation
Recommended UsageAlthough many buffers can be employed for reconstituting and diluting this lectin, 10 mM HEPES buffered saline, pH 8.5, 0.1 mM CaCl2 is recommended. For preserving solutions stored at 4 ºC, 0.08% sodium azide can be used.
Recommended Storage2-8 °C
ConjugateUnconjugated
Sugar SpecificityN-Acetylgalactosamine

Documents

Citations

Technical Information

Inhibiting/Eluting Sugar: 200 mM N-acetylgalactosamine

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]]> Maclura Pomifera Lectin (MPL), Unconjugated https://staging.vectorlabs.com/products/unconj-maclura-pomifera-lectin-mpl/ Thu, 27 Apr 2023 17:19:30 +0000 https://staging.vectorlabs.com/?post_type=product&p=12497 Description

There appear to be two different chains in combinations giving rise to five isolectins. The carbohydrate specificity of either type of subunit appears to be the same, preferring α-linked N-acetylgalactosamine structures.

Inhibiting Sugar: 500 mM galactose

Unit Size 5 mg
Applications Glycobiology, Mitogenic Stimulation
Recommended Usage Although many buffers can be employed for reconstituting and diluting this lectin, 10 mM HEPES buffered saline, pH 8.5, 0.1 mM CaCl2 is recommended. For preserving solutions stored at 4 ºC, 0.08% sodium azide can be used.
Recommended Storage 2-8 °C
Conjugate Unconjugated
Sugar Specificity Galactose, N-Acetylgalactosamine

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Description

There appear to be two different chains in combinations giving rise to five isolectins. The carbohydrate specificity of either type of subunit appears to be the same, preferring α-linked N-acetylgalactosamine structures.

Inhibiting Sugar: 500 mM galactose

Specifications

Unit Size5 mg
ApplicationsGlycobiology, Mitogenic Stimulation
Recommended UsageAlthough many buffers can be employed for reconstituting and diluting this lectin, 10 mM HEPES buffered saline, pH 8.5, 0.1 mM CaCl2 is recommended. For preserving solutions stored at 4 ºC, 0.08% sodium azide can be used.
Recommended Storage2-8 °C
ConjugateUnconjugated
Sugar SpecificityGalactose, N-Acetylgalactosamine

Documents

Citations

The post Maclura Pomifera Lectin (MPL), Unconjugated appeared first on VectorLabs.

]]> Maackia Amurensis Lectin I (MAL I), Unconjugated https://staging.vectorlabs.com/products/unconj-maackia-amurensis-lectin-i-mal-i/ Thu, 27 Apr 2023 17:10:30 +0000 https://staging.vectorlabs.com/?post_type=product&p=12483 Description

Maackia amurensis lectin I binds gal (β-1,4) glcNAc but tolerates substitution of N-acetyllactosamine with sialic acid at the 3 position of galactose. However, MAL I does not appear to bind this structure when substitution with sialic acid is on the 6 position of galactose.

Specifications

Unit Size 5 mg
Applications Glycobiology, Mitogenic Stimulation
Recommended Usage Although many buffers can be employed for reconstituting and diluting this lectin, 10 mM HEPES buffered saline, pH 8.5, 0.1 mM CaCl2 is recommended. For preserving solutions stored at 4 ºC, 0.08% sodium azide can be used.
Recommended Storage 2-8 °C
Conjugate Unconjugated
Sugar Specificity Galactose, Lactose

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Description

Maackia amurensis lectin I binds gal (β-1,4) glcNAc but tolerates substitution of N-acetyllactosamine with sialic acid at the 3 position of galactose. However, MAL I does not appear to bind this structure when substitution with sialic acid is on the 6 position of galactose.

Specifications

Unit Size5 mg
ApplicationsGlycobiology, Mitogenic Stimulation
Recommended UsageAlthough many buffers can be employed for reconstituting and diluting this lectin, 10 mM HEPES buffered saline, pH 8.5, 0.1 mM CaCl2 is recommended. For preserving solutions stored at 4 ºC, 0.08% sodium azide can be used.
Recommended Storage2-8 °C
ConjugateUnconjugated
Sugar SpecificityGalactose, Lactose

Documents

Citations

Technical Information

This lectin is the leucoagglutinin or mitogenic isolectin from Maackia seeds.

Geisler, C and Jarvis, D.L. Glycobiology, vol. 21, no. 8 pp. 988-993, 2011

Inhibiting Sugar: 200 mM lactose

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]]> Bauhinia Purpurea Lectin (BPL), Unconjugated https://staging.vectorlabs.com/products/unconj-bauhinia-purpurea-lectin-bpl/ Thu, 27 Apr 2023 17:03:45 +0000 https://staging.vectorlabs.com/?post_type=product&p=12468 Description

BPL binding appears to be highest for glycoconjugates containing galactosyl (β-1,3)N-acetylgalactosamine structures but oligosaccharides with a terminal α-linked N-acetylgalactosamine can also bind. Although binding specificity is similar to that of peanut agglutinin, tissue staining patterns of these two lectins are distinct.

Inhibiting/Eluting Sugar: 100 mM lactose

Specifications

More Information
Unit Size 5 mg
Applications Glycobiology, Mitogenic Stimulation
Recommended Usage Although many buffers can be employed for reconstituting and diluting this lectin, 10 mM HEPES buffered saline, pH 8.5, 0.1 mM CaCl2 is recommended. For preserving solutions stored at 4 ºC, 0.08% sodium azide can be used.
Recommended Storage 2-8 °C
Conjugate Unconjugated
Sugar Specificity N-Acetylgalactosamine, Lactose

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Description

BPL binding appears to be highest for glycoconjugates containing galactosyl (β-1,3)N-acetylgalactosamine structures but oligosaccharides with a terminal α-linked N-acetylgalactosamine can also bind. Although binding specificity is similar to that of peanut agglutinin, tissue staining patterns of these two lectins are distinct.

Inhibiting/Eluting Sugar: 100 mM lactose

Specifications

More Information
Unit Size5 mg
ApplicationsGlycobiology, Mitogenic Stimulation
Recommended UsageAlthough many buffers can be employed for reconstituting and diluting this lectin, 10 mM HEPES buffered saline, pH 8.5, 0.1 mM CaCl2 is recommended. For preserving solutions stored at 4 ºC, 0.08% sodium azide can be used.
Recommended Storage2-8 °C
ConjugateUnconjugated
Sugar SpecificityN-Acetylgalactosamine, Lactose

Documents

Citations

The post Bauhinia Purpurea Lectin (BPL), Unconjugated appeared first on VectorLabs.

]]> Maackia Amurensis Lectin II (MAL II), Unconjugated https://staging.vectorlabs.com/products/unconj-maackia-amurensis-lectin-ii-mal-ii/ Thu, 27 Apr 2023 16:58:47 +0000 https://staging.vectorlabs.com/?post_type=product&p=12455 Description

Although the specificity of this lectin is not well defined, MAL II appears to bind only particular carbohydrate structures that contain sialic acid. Unlike Sambucus nigra lectin (SNA) which seems to prefer structures with (α-2,6) linked sialic acid, MAL II appears to bind sialic acid in an (α-2,3) linkage. Tissue staining patterns are also very different among MAL I, SNA and MAL II.

Specifications

Unit Size 2 mg
Applications Glycobiology, Mitogenic Stimulation
Recommended Storage 2-8 °C
Solution 10 mM HEPES, pH 7.5, 0.15 M NaCl, 0.08% sodium azide, 0.1mM CaCl2.
Concentration 2 mg/ml
Conjugate Unconjugated
Sugar Specificity Sialic Acid

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Description

Although the specificity of this lectin is not well defined, MAL II appears to bind only particular carbohydrate structures that contain sialic acid. Unlike Sambucus nigra lectin (SNA) which seems to prefer structures with (α-2,6) linked sialic acid, MAL II appears to bind sialic acid in an (α-2,3) linkage. Tissue staining patterns are also very different among MAL I, SNA and MAL II.

Specifications

Unit Size2 mg
ApplicationsGlycobiology, Mitogenic Stimulation
Recommended Storage2-8 °C
Solution10 mM HEPES, pH 7.5, 0.15 M NaCl, 0.08% sodium azide, 0.1mM CaCl2.
Concentration2 mg/ml
ConjugateUnconjugated
Sugar SpecificitySialic Acid

Documents

Citations

Technical Information

Maackia amurensis lectin II is a glycoprotein consisting of two subunits each of which is composed of disulfide-linked chains.

Geisler, C and Jarvis, D.L. Glycobiology, vol. 21, no. 8 pp. 988-993, 2011

Inhibitor: Human glycophorin

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]]> Datura Stramonium Lectin (DSL), Unconjugated https://staging.vectorlabs.com/products/unconj-datura-stramonium-lectin-dsl/ Thu, 27 Apr 2023 14:35:13 +0000 https://staging.vectorlabs.com/?post_type=product&p=12426 Description

The carbohydrate binding site recognizes (β-1,4) linked N-acetylglucosamine oligomers, preferring chitobiose or chitotriose over a single N-acetylglucosamine residue. This lectin binds well in the acidic pH range but its affinity decreases above pH 8.0. DSL also binds well to N-acetyllactosamine and oligomers containing repeating N-acetyllactosamine sequences. A branched pentasaccharide including two N-acetyllactosamine disaccharides linked to mannose (β-1,6) and (β-1,2) was reported to be the most potent inhibitor of agglutination.

Specifications

More Information
Unit Size 5 mg
Applications Glycobiology, Mitogenic Stimulation
Recommended Usage Though many buffers can be employed for reconstituting and diluting this lectin, 10 mM HEPES buffered saline, pH 8.5, 0.1 mM CaCl2 is recommended. For preserving solutions stored at 4 ºC, 0.08% sodium azide can be used. Aggregation may occur with time if stored at concentrations greater than 2 mg/ml.
Recommended Storage 2-8 °C; for long term storage, aliquots may be stored frozen or preserved with 0.08% sodium azide in the recommended buffer and stored at 2-8 °C
Conjugate Unconjugated
Sugar Specificity [GlcNAc]1-3, N-Acetylglucosamine

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Description

The carbohydrate binding site recognizes (β-1,4) linked N-acetylglucosamine oligomers, preferring chitobiose or chitotriose over a single N-acetylglucosamine residue. This lectin binds well in the acidic pH range but its affinity decreases above pH 8.0. DSL also binds well to N-acetyllactosamine and oligomers containing repeating N-acetyllactosamine sequences. A branched pentasaccharide including two N-acetyllactosamine disaccharides linked to mannose (β-1,6) and (β-1,2) was reported to be the most potent inhibitor of agglutination.

Specifications

More Information
Unit Size5 mg
ApplicationsGlycobiology, Mitogenic Stimulation
Recommended UsageThough many buffers can be employed for reconstituting and diluting this lectin, 10 mM HEPES buffered saline, pH 8.5, 0.1 mM CaCl2 is recommended. For preserving solutions stored at 4 ºC, 0.08% sodium azide can be used. Aggregation may occur with time if stored at concentrations greater than 2 mg/ml.
Recommended Storage2-8 °C; for long term storage, aliquots may be stored frozen or preserved with 0.08% sodium azide in the recommended buffer and stored at 2-8 °C
ConjugateUnconjugated
Sugar Specificity[GlcNAc]1-3, N-Acetylglucosamine

Documents

Citations

Technical Information

DSL contains two chains of 40 kDa and 46 kDa joined by disulfide bonds. This lectin is free of a reported 32 kDa contaminant protein. 

Inhibiting/Eluting Sugar: Chitin Hydrolysate

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