Mannose – VectorLabs https://staging.vectorlabs.com From linker design and synthesis, bioconjugated design and manufacturing, biomolecule labeling and functionalization, and detection system and solutions, our team is here to help you move forward, with impact. Wed, 22 Oct 2025 17:14:52 +0000 en-US hourly 1 https://wordpress.org/?v=6.9 https://staging.vectorlabs.com/wp-content/uploads/2024/11/V-favicon-02-100x100.png Mannose – VectorLabs https://staging.vectorlabs.com 32 32 Lens Culinaris Agglutinin (LCA), DyLight® 649 https://staging.vectorlabs.com/products/dylight-649-labeled-lens-culinaris-agglutinin/ Tue, 02 May 2023 16:04:48 +0000 https://staging.vectorlabs.com/?post_type=product&p=14318 Description

Lens culinaris agglutinin (LCA) recognizes sequences containing α-linked mannose residues but recognizes additional sugars as part of the receptor structure, giving it a narrower specificity than Con A. An α-linked fucose residue attached to the N-acetylchitobiose portion of the core oligosaccharide significantly enhances affinity. By exploiting this increased specificity, glycoproteins and glycopeptides can be subfractionated with LCA after initial isolation with Con A.

DyLight™ 649 labeled LCA has an appropriate number of bound fluorochromes, which provides the optimum staining characteristics for this lectin. This conjugate is supplied essentially free of unconjugated fluorochromes.

  • Excitation maximum: 655 nm
  • Emission maximum: 670 nm
  • Color: Far red

 

Lens Culinaris Agglutinin (LCA), DyLight™ 649 is provided in a 1 mL aliquot at a concentration of 1 mg active conjugate/ml.

Specifications

Unit Size 1 mg
Applications Immunofluorescence, Glycobiology
Recommended Usage The recommended concentration range for use is 5-20 µg/ml. If a precipitate forms upon long-term storage, warm to 37 ºC.
Recommended Storage 2-8 °C
Maximum Excitation 655 nm
Maximum Emission 670 nm
Solution 10 mM HEPES, 0.15 M NaCl, pH 7.5, 0.08% sodium azide, 0.1 mM CaCl2 , 0.01 mM MnCl2
Concentration 1 mg active conjugate/ml
Conjugate DyLight 649
Color of Fluorescence Far Red
Sugar Specificity Mannose, Glucose

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Description

Lens culinaris agglutinin (LCA) recognizes sequences containing α-linked mannose residues but recognizes additional sugars as part of the receptor structure, giving it a narrower specificity than Con A. An α-linked fucose residue attached to the N-acetylchitobiose portion of the core oligosaccharide significantly enhances affinity. By exploiting this increased specificity, glycoproteins and glycopeptides can be subfractionated with LCA after initial isolation with Con A.

DyLight™ 649 labeled LCA has an appropriate number of bound fluorochromes, which provides the optimum staining characteristics for this lectin. This conjugate is supplied essentially free of unconjugated fluorochromes.

  • Excitation maximum: 655 nm
  • Emission maximum: 670 nm
  • Color: Far red

Lens Culinaris Agglutinin (LCA), DyLight™ 649 is provided in a 1 mL aliquot at a concentration of 1 mg active conjugate/ml.

Specifications

Unit Size1 mg
ApplicationsImmunofluorescence, Glycobiology
Recommended UsageThe recommended concentration range for use is 5-20 µg/ml. If a precipitate forms upon long-term storage, warm to 37 ºC.
Recommended Storage2-8 °C
Maximum Excitation655 nm
Maximum Emission670 nm
Solution10 mM HEPES, 0.15 M NaCl, pH 7.5, 0.08% sodium azide, 0.1 mM CaCl2 , 0.01 mM MnCl2
Concentration1 mg active conjugate/ml
ConjugateDyLight 649
Color of FluorescenceFar Red
Sugar SpecificityMannose, Glucose

Documents

Citations

Technical Information

Lens culinaris agglutinin is composed of four subunits – two of about 17 kDa and two of 8 kDa. 

LCA has been found to be one of the most effective agents in preventing skin allograft rejection in model systems. LCA has been used to purify numerous glycoproteins, including immunoglobulins, histocompatibility antigens, and α2-macroglobulin.

Accompanying each fluorescent lectin is an analysis data sheet summarizing the results of our quality control tests and providing pertinent information on the product. All of these reagents are supplied as solutions preserved with sodium azide. 

Inhibiting/Eluting Sugar: mixture of 200 mM α-methylmannoside/200 mM α-methylglucoside

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]]> Lens Culinaris Agglutinin (LCA), Unconjugated https://staging.vectorlabs.com/products/unconjugated-lens-culinaris-agglutinin/ Tue, 02 May 2023 14:31:38 +0000 https://staging.vectorlabs.com/?post_type=product&p=14116 Description

LCA recognizes sequences containing α-linked mannose residues but recognizes additional sugars as part of the receptor structure, giving it a narrower specificity than Con A. An α-linked fucose residue attached to the N-acetylchitobiose portion of the core oligosaccharide significantly enhances affinity. By exploiting this narrower specificity, glycoproteins and glycopeptides can be subfractionated with LCA after initial isolation with Con A.

Specifications

 
Unit Size 10 mg, 25 mg
Applications Immunohistochemistry / Immunocytochemistry, Immunofluorescence, Blotting Applications, Glycobiology, Mitogenic Stimulation
Recommended Usage Although many buffers can be employed for reconstituting and diluting this lectin, 10 mM HEPES buffered saline, pH 8.5, 0.1 mM CaCl2 is recommended. For preserving solutions stored at 4 ºC, 0.08% sodium azide can be used.
Recommended Storage 2-8 °C
Inhibiting and/or Eluting Sugar mixture of 200 mM α−methylmannoside/200 mM α−methylglucoside
Conjugate Unconjugated
Sugar Specificity Mannose, Glucose

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Description

LCA recognizes sequences containing α-linked mannose residues but recognizes additional sugars as part of the receptor structure, giving it a narrower specificity than Con A. An α-linked fucose residue attached to the N-acetylchitobiose portion of the core oligosaccharide significantly enhances affinity. By exploiting this narrower specificity, glycoproteins and glycopeptides can be subfractionated with LCA after initial isolation with Con A.

Specifications

 
Unit Size10 mg, 25 mg
ApplicationsImmunohistochemistry / Immunocytochemistry, Immunofluorescence, Blotting Applications, Glycobiology, Mitogenic Stimulation
Recommended UsageAlthough many buffers can be employed for reconstituting and diluting this lectin, 10 mM HEPES buffered saline, pH 8.5, 0.1 mM CaCl2 is recommended. For preserving solutions stored at 4 ºC, 0.08% sodium azide can be used.
Recommended Storage2-8 °C
Inhibiting and/or Eluting Sugarmixture of 200 mM α−methylmannoside/200 mM α−methylglucoside
ConjugateUnconjugated
Sugar SpecificityMannose, Glucose

Documents

Citations

Technical Information

Lens culinaris agglutinin is composed of four subunits – two of about 17 kDa and two of 8 kDa. LCA has been found to be one of the most effective agents in preventing skin allograft rejection in model systems. LCA has been used to purify numerous glycoproteins, including immunoglobulins, histocompatibility antigens, and α2-macroglobulin.

Inhibiting/Eluting Sugar: mixture of 200 mM α-methylmannoside/200 mM α-methylglucoside

The post Lens Culinaris Agglutinin (LCA), Unconjugated appeared first on VectorLabs.

]]> Galanthus Nivalis Lectin (GNL), Agarose bound https://staging.vectorlabs.com/products/agarose-bound-galanthus-nivalis-lectin-gnl/ Tue, 02 May 2023 04:46:53 +0000 https://staging.vectorlabs.com/?post_type=product&p=14020 Description

Agarose bound Galanthus nivalis lectin is prepared using our affinity-purified lectins. Galanthus nivalis lectin, unlike most mannose-specific lectins, is not a metalloprotein and does not require Ca++ or Mn++ for binding. Binding seems to be preferentially directed toward structures containing (α-1,3) mannose residues.

In contrast to most mannose-binding lectins, GNL will not bind α-linked glucose. Reports indicate that this lectin binds rat and mouse IgM but not IgG. The only protein from human serum reported to bind to this lectin is α2-macroglobulin. GNL binds to many viral glycoproteins.

Features:

  • Bead diameter ranges in size from 45-165 microns
  • Matrix is stable in solutions at pH 3-11 as well as many organic solvents
  • Immobilized lectins are prepared using affinity purified lectins
  • Covalent attachment preserves lectin activity and minimizes conformational changes that might result in nonspecific or hydrophobic interactions
  • Hydrophilic spacer arm is inserted between the lectin and the matrix
  • Conjugated proteins are not leached off the beads by Tris or other routinely used buffers
  • No residual charges present after conjugation.  This minimizes non-specific binding to the matrix
  • Product supplied as a 1:1 suspension in buffer
  • 3 mg lectin/ml gel
  • Inhibiting/Eluting Sugar: 100 mM – 200 mM α-methylmannoside or Glycoprotein Eluting Solution (ES-1100)

Specifications

Unit Size 5 ml
Applications Glycobiology, Affinity Chromatography
Recommended Storage 2-8 °C DO NOT FREEZE
Solution 10 mM HEPES, pH 7.5, 0.15 M NaCl, 0.1 mM CaCl2, 0.01 mM MnCl2, 20 mM mannose, 0.08% sodium azide
Recommended Usage Wash gel thoroughly with buffer before use to remove sugar added to stabilize the lectin. Recommended product for eluting glycoconjugates bound to this agarose-lectin: Glycoprotein Eluting Solution, Cat. No. ES-1100. Alternatively, 0.1 M α methyl mannoside can be used.For those glycoconjugates having a very high affinity for GNL, it may be necessary to lower the pH of the eluting sugar solution to pH 4.0 with acetic acid and increase the concentration of the α methyl mannoside to 0.5 M. After use, wash the gel with several column volumes of buffered saline, then resuspend gel in buffered saline containing 0.08% sodium azide for storage.
Matrix Conjugate Lectins
Sugar Specificity Mannose
Conjugate Agarose

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Description

Agarose bound Galanthus nivalis lectin is prepared using our affinity-purified lectins. Galanthus nivalis lectin, unlike most mannose-specific lectins, is not a metalloprotein and does not require Ca++ or Mn++ for binding. Binding seems to be preferentially directed toward structures containing (α-1,3) mannose residues. 

In contrast to most mannose-binding lectins, GNL will not bind α-linked glucose. Reports indicate that this lectin binds rat and mouse IgM but not IgG. The only protein from human serum reported to bind to this lectin is α2-macroglobulin. GNL binds to many viral glycoproteins.

Features:

  • Bead diameter ranges in size from 45-165 microns
  • Matrix is stable in solutions at pH 3-11 as well as many organic solvents
  • Immobilized lectins are prepared using affinity purified lectins
  • Covalent attachment preserves lectin activity and minimizes conformational changes that might result in nonspecific or hydrophobic interactions
  • Hydrophilic spacer arm is inserted between the lectin and the matrix
  • Conjugated proteins are not leached off the beads by Tris or other routinely used buffers
  • No residual charges present after conjugation.  This minimizes non-specific binding to the matrix
  • Product supplied as a 1:1 suspension in buffer
  • 3 mg lectin/ml gel
  • Inhibiting/Eluting Sugar: 100 mM – 200 mM α-methylmannoside or Glycoprotein Eluting Solution (ES-1100)

Specifications

Unit Size5 ml
ApplicationsGlycobiology, Affinity Chromatography
Recommended Storage2-8 °C DO NOT FREEZE
Solution10 mM HEPES, pH 7.5, 0.15 M NaCl, 0.1 mM CaCl2, 0.01 mM MnCl2, 20 mM mannose, 0.08% sodium azide
Recommended UsageWash gel thoroughly with buffer before use to remove sugar added to stabilize the lectin. Recommended product for eluting glycoconjugates bound to this agarose-lectin: Glycoprotein Eluting Solution, Cat. No. ES-1100. Alternatively, 0.1 M α methyl mannoside can be used.For those glycoconjugates having a very high affinity for GNL, it may be necessary to lower the pH of the eluting sugar solution to pH 4.0 with acetic acid and increase the concentration of the α methyl mannoside to 0.5 M. After use, wash the gel with several column volumes of buffered saline, then resuspend gel in buffered saline containing 0.08% sodium azide for storage.
Matrix ConjugateLectins
Sugar SpecificityMannose
ConjugateAgarose

Documents

Product FAQs

Our agarose lectin products are supplied as hydrated matrix solutions in amber glass bottles. The agarose (bead) material will settle and you will see two phases in the tube supplied. The upper phase is buffer. A column can be prepared in a commercial plastic device such as Bio-Rad Cat # 732-6008 or an inverted Pasteur pipet with glass wool lightly packed in the neck to retain the agarose. 1) Draw (pipet) the desired amount of settled agarose-lectin (gel) from the stock bottle into the prepared column and let the buffer drain by gravity.(Sometimes an air bubble in the column tip prevents flow; tapping the column should get the flow started). 2) Wash the gel with 10 column volumes of buffer, such as HBS (10 mM HEPES, 0.15 M NaCl, pH 7.5) and discard the flow through. 3) Place a collection vessel (e.g. glass test tube) under the column tip and apply the glycoprotein-containing solution.Allow the solution to drain through using gravity. We recommend against pushing or pulling the material through the column. Retain the flow through material until the desired binding has been confirmed. 4) After sample application, wash column with 2-3 column volumes of buffer (or until the absorbance at 280nm is reduced to a satisfactory level) to remove unbound materials before elution. 5) Place a fresh collection vessel under the column tip.Apply the eluting solution again letting gravity do the work of moving the solution over the column. Note that in some cases, several column volumes of eluting solution may be required to achieved adequate release of bound material. 6) Following elution, the column can be prepared for reuse by washing with 10 column volumes of buffer. 7) If the column is to be stored, equilibrate the column with buffer containing 0.08% sodium azide. Cover the column with a plastic wrap, or similar, to prevent desiccation and keep at 4 degrees Celsius. The column will be stable for many months when stored under these conditions.

The pH should be near neutral, the maximum pressure for packing the resin is 10 psi, and the maximum flow rate 3.5 ml/min.

Citations

Technical Information

Our coupling method provides several advantages over the traditional cyanogen bromide procedure:

  • Maximum carbohydrate binding activity of the coupled lectins is retained
  • Linkage is stable over a range of pH values
  • Conjugated proteins are not leached off the beads by Tris or other routinely used buffers
  • No residual charges are present after conjugation. This minimizes non-specific binding to the matrix

Our agarose bound lectins are supplied at a constant concentration of lectin per ml of settled beads. The concentration for each lectin is selected to achieve the highest glycoconjugate binding capacity per mg of lectin present in the beads. Each lot is tested for its binding capacity using glycoproteins known to bind the lectin. This provides a guideline for the user and assures the quality of our agarose bound lectins.

Inhibiting/Eluting Sugar: 100 mM – 200 mM α-methylmannoside

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]]> Concanavalin A (Con A), Agarose bound https://staging.vectorlabs.com/products/agarose-bound-concanavalin-a-con-a/ Tue, 02 May 2023 02:40:58 +0000 https://staging.vectorlabs.com/?post_type=product&p=13951

Description

Agarose bound Con A is prepared using our affinity-purified lectins. Con A recognizes α-linked mannose present as part of a core oligosaccharide in many serum and membrane glycoproteins. At neutral and alkaline pH, Con A exists as a tetramer of four identical subunits; below pH 5.6, Con A dissociates into active dimers of 52 kDa. Acetylation, succinylation, or other derivatizations can also produce stable forms with dimeric structures. (See succinylated Con A). Nicks in the sequence are often present in the purest preparations due to hydrolytic damage within the seeds.

Features:

  • Bead diameter ranges in size from 45-165 microns
  • Matrix is stable in solutions at pH 3-11 as well as many organic solvents
  • Immobilized lectins are prepared using affinity purified lectins
  • Covalent attachment preserves lectin activity and minimizes conformational changes that might result in nonspecific or hydrophobic interactions
  • Hydrophilic spacer arm is inserted between the lectin and the matrix
  • Conjugated proteins are not leached off the beads by Tris or other routinely used buffers
  • No residual charges present after conjugation.  This minimizes non-specific binding to the matrix
  • Product supplied as a 1:1 suspension in buffer

Specifications

Unit Size 10 ml, 100 ml
Applications Glycobiology, Affinity Chromatography
Recommended Storage 2-8 °C DO NOT FREEZE
Solution 10 mM HEPES, pH 7.5, 0.15 M NaCl, 0.1 mM CaCl2, 0.01 mM MnCl2, 20 mM glucose, 0.08% sodium azide
Recommended Usage Wash gel thoroughly with buffer before use to remove sugar added to stabilize the lectin. Recommended product for eluting glycoconjugates bound to this agarose-lectin: Glycoprotein Eluting Solution, Cat. No. ES-1100. Alternatively, a 0.2 M α-methyl mannoside/0.2 M α-methyl glucoside mixture can be used. After use, wash the gel with several column volumes of buffered saline, then resuspend gel in buffered saline containing 0.08% sodium azide for storage.
Matrix Conjugate Lectins
Sugar Specificity Mannose, Glucose
Conjugate Agarose

The post Concanavalin A (Con A), Agarose bound appeared first on VectorLabs.

]]>

Description

Agarose bound Con A is prepared using our affinity-purified lectins. Con A recognizes α-linked mannose present as part of a core oligosaccharide in many serum and membrane glycoproteins. At neutral and alkaline pH, Con A exists as a tetramer of four identical subunits; below pH 5.6, Con A dissociates into active dimers of 52 kDa. Acetylation, succinylation, or other derivatizations can also produce stable forms with dimeric structures. (See succinylated Con A). Nicks in the sequence are often present in the purest preparations due to hydrolytic damage within the seeds.

Features:

  • Bead diameter ranges in size from 45-165 microns
  • Matrix is stable in solutions at pH 3-11 as well as many organic solvents
  • Immobilized lectins are prepared using affinity purified lectins
  • Covalent attachment preserves lectin activity and minimizes conformational changes that might result in nonspecific or hydrophobic interactions
  • Hydrophilic spacer arm is inserted between the lectin and the matrix
  • Conjugated proteins are not leached off the beads by Tris or other routinely used buffers
  • No residual charges present after conjugation.  This minimizes non-specific binding to the matrix
  • Product supplied as a 1:1 suspension in buffer

Specifications

Unit Size10 ml, 100 ml
ApplicationsGlycobiology, Affinity Chromatography
Recommended Storage2-8 °C DO NOT FREEZE
Solution10 mM HEPES, pH 7.5, 0.15 M NaCl, 0.1 mM CaCl2, 0.01 mM MnCl2, 20 mM glucose, 0.08% sodium azide
Recommended UsageWash gel thoroughly with buffer before use to remove sugar added to stabilize the lectin. Recommended product for eluting glycoconjugates bound to this agarose-lectin: Glycoprotein Eluting Solution, Cat. No. ES-1100. Alternatively, a 0.2 M α-methyl mannoside/0.2 M α-methyl glucoside mixture can be used. After use, wash the gel with several column volumes of buffered saline, then resuspend gel in buffered saline containing 0.08% sodium azide for storage.
Matrix ConjugateLectins
Sugar SpecificityMannose, Glucose
ConjugateAgarose

Documents

Product FAQs

Our agarose lectin products are supplied as hydrated matrix solutions in amber glass bottles. The agarose (bead) material will settle and you will see two phases in the tube supplied. The upper phase is buffer. A column can be prepared in a commercial plastic device such as Bio-Rad Cat # 732-6008 or an inverted Pasteur pipet with glass wool lightly packed in the neck to retain the agarose. 1) Draw (pipet) the desired amount of settled agarose-lectin (gel) from the stock bottle into the prepared column and let the buffer drain by gravity.(Sometimes an air bubble in the column tip prevents flow; tapping the column should get the flow started). 2) Wash the gel with 10 column volumes of buffer, such as HBS (10 mM HEPES, 0.15 M NaCl, pH 7.5) and discard the flow through. 3) Place a collection vessel (e.g. glass test tube) under the column tip and apply the glycoprotein-containing solution.Allow the solution to drain through using gravity. We recommend against pushing or pulling the material through the column. Retain the flow through material until the desired binding has been confirmed. 4) After sample application, wash column with 2-3 column volumes of buffer (or until the absorbance at 280nm is reduced to a satisfactory level) to remove unbound materials before elution. 5) Place a fresh collection vessel under the column tip.Apply the eluting solution again letting gravity do the work of moving the solution over the column. Note that in some cases, several column volumes of eluting solution may be required to achieved adequate release of bound material. 6) Following elution, the column can be prepared for reuse by washing with 10 column volumes of buffer. 7) If the column is to be stored, equilibrate the column with buffer containing 0.08% sodium azide. Cover the column with a plastic wrap, or similar, to prevent desiccation and keep at 4 degrees Celsius. The column will be stable for many months when stored under these conditions.

The pH should be near neutral, the maximum pressure for packing the resin is 10 psi, and the maximum flow rate 3.5 ml/min.

Citations

Technical Information

Con A requires calcium or manganese ions at each of its four saccharide binding sites. Although these divalent metal ions are bound tightly to the polypeptide structure, buffers which can bind calcium (such as phosphate) generally should be avoided in diluting Con A, since a gradual loss in activity may occur.

Agarose bound* Con A is prepared using our affinity-purified lectins. Heat stable, cross-linked 4% agarose beads with a molecular weight exclusion limit of about 2×107 daltons are used as the solid-phase matrix to which the lectins are covalently coupled. 

This coupling method provides several advantages over the traditional cyanogen bromide procedure:

  • Maximum carbohydrate binding activity of the coupled lectins is retained
  • Linkage is stable over a range of pH values

Our agarose bound lectins are supplied at a constant concentration of lectin per ml of settled beads. The concentration for each lectin is selected to achieve the highest glycoconjugate binding capacity per mg of lectin present in the beads. Each lot is tested for its binding capacity using glycoproteins known to bind the lectin. This provides a guideline for the user and assures the quality of our agarose bound lectins.

Inhibiting/Eluting Sugar: mixture of 200 mM α-methylmannoside/200 mM α-methylglucoside or Glycoprotein Eluting Solution (ES-1100)

*6 mg lectin/ml gel

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]]> Glysite™ Scout Glycan Screening Kit, Immunofluorescence 488 https://staging.vectorlabs.com/products/glysite-scout-screening-kit-488/ Sun, 30 Apr 2023 03:33:40 +0000 https://staging.vectorlabs.com/?post_type=product&p=13446 Description

Glysite Scout Glycan Screening Kit, Immunofluorescence 488 is a fully integrated kit for the detection of glycan expression in tissue sections. Included in the kit is a diverse panel of glycan binders (lectins) ideal for the preliminary screening of tissue samples with unknown glycosylation changes with specificity to mannose, complex N-glycan, core O-glycan, fucose, sialic acid, sulfation, GlcNAc, chitin, galactose, and LacNAc.

Validated on human and mouse tissue—including FFPE tissue, across a range of tissue types including colon, lung, spleen, kidney, liver, pancreas, testes, heart, bladder, and uterus—this versatile kit is flexible to meet your needs. Glysite Scout Glycan Screening Kits, Immunofluorescence are available with 3 fluorophore options, 488 (GSK-3000), 594 (GSK-2000), and 649 (GSK-1000).

Specifications

Unit Size 1 kit
Applications Immunofluorescence, Glycobiology
Recommended Storage Store reagents in original bottles at 2–8 °C. Do not freeze the kit.
Conjugate DyLight 488
Sugar Specificity Mannose, Galactose, Fucose, Sialic Acid

Kit Components

This glycan screening kit is designed to provide the investigator with a panel of eight biotinylated lectin conjugates. The lectins have been selected to offer a variety of sugar specificities and are of the same high quality as the reagents offered individually.

Glysite Scout Glycan Screening Kit, Immunofluorescence 488 contains the following biotinylated lectins:

  • AAL (Aleuria Aurantia)
  • ECL, ECA (Erythrina Cristagalli)
  • GNL (Galanthus Nivalis)
  • Jacalin
  • MAL II (Maackia Amurensis II)
  • PHA-L (Phaseolus Vulgaris Leucoagglutinin)
  • WFA, WFL (Wisteria Fluoribunda)
  • WGA (Wheat Germ Agglutinin)

 

In addition to the glycan binders, the kit includes optimized immunofluorescence reagents for glycan detection, including blocking solutions, DyLight™ streptavidin, and VECTASHIELD Vibrance® Antifade Mounting Medium with DAPI.

The post Glysite™ Scout Glycan Screening Kit, Immunofluorescence 488 appeared first on VectorLabs.

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Resources

Description

Glysite Scout Glycan Screening Kit, Immunofluorescence 488 is a fully integrated kit for the detection of glycan expression in tissue sections. Included in the kit is a diverse panel of glycan binders (lectins) ideal for the preliminary screening of tissue samples with unknown glycosylation changes with specificity to mannose, complex N-glycan, core O-glycan, fucose, sialic acid, sulfation, GlcNAc, chitin, galactose, and LacNAc.

Validated on human and mouse tissue—including FFPE tissue, across a range of tissue types including colon, lung, spleen, kidney, liver, pancreas, testes, heart, bladder, and uterus—this versatile kit is flexible to meet your needs. Glysite Scout Glycan Screening Kits, Immunofluorescence are available with 3 fluorophore options, 488 (GSK-3000), 594 (GSK-2000), and 649 (GSK-1000).

Specifications

Unit Size1 kit
ApplicationsImmunofluorescence, Glycobiology
Recommended StorageStore reagents in original bottles at 2–8 °C. Do not freeze the kit.
ConjugateDyLight 488
Sugar SpecificityMannose, Galactose, Fucose, Sialic Acid

Kit Components

This glycan screening kit is designed to provide the investigator with a panel of eight biotinylated lectin conjugates. The lectins have been selected to offer a variety of sugar specificities and are of the same high quality as the reagents offered individually.

Glysite Scout Glycan Screening Kit, Immunofluorescence 488 contains the following biotinylated lectins:

  • AAL (Aleuria Aurantia)
  • ECL, ECA (Erythrina Cristagalli)
  • GNL (Galanthus Nivalis)
  • Jacalin
  • MAL II (Maackia Amurensis II)
  • PHA-L (Phaseolus Vulgaris Leucoagglutinin)
  • WFA, WFL (Wisteria Fluoribunda)
  • WGA (Wheat Germ Agglutinin)

In addition to the glycan binders, the kit includes optimized immunofluorescence reagents for glycan detection, including blocking solutions, DyLight™ streptavidin, and VECTASHIELD Vibrance® Antifade Mounting Medium with DAPI. 

Documents

Product FAQs

Virtually any tissue type and species are suitable for use with a Glysite Scout Glycan Screening Kit, Immunofluorescence, as glycans are ubiquitous in nature and in life. The kit has been validated on human and mouse tissue—including FFPE tissue, across a range of organs, including colon, lung, spleen, kidney, liver, pancreas, testes, heart, bladder, and uterus.

Each of the three Glysite Scout Glycan Screening Kits, Immunofluorescence contain the same lectins but with a different fluorescent streptavidin detection reagent, offering a choice of Dylight™ 488 (GSK-3000), Dylight™ 594 (GSK-2000), or Dylight™ 649 (GSK-1000). This allows users to choose the kit which best matches the capabilities of their fluorescent microscope and also provides flexibility for multiplexing with other fluorescent detection reagents they might already be using. 

VECTASHIELD Vibrance is an antifade medium for immunofluorescence applications with setting properties, which means the coverslip is immobilized after curing and does not require sealing. Additionally, slides can be stored at room temperature, enabling extended archiving time. VECTASHIELD PLUS does not have setting properties and remains molten, and slides must be stored at 2–8°C. Both VECTASHIELD Vibrance and VECTASHIELD PLUS have superior antifade/anti-photobleaching properties across the spectrum and are compatible with commonly used fluorophores. 

The need for an antigen retrieval step in a staining protocol depends on multiple variables, including the target glycan and its location in the specimen, the glycan-binder, the type of tissue, and the method and length of fixation. Investigators should run controls with and without antigen retrieval to determine if this step is needed for their specimen. 

Tissue components such as red blood cells, elastin, and collagen are strongly fluorescent, particularly in FFPE sections, making it difficult to discern between relevant signal and background. Formalin fixation tends to induce a significant amount of fluorescence to a specimen. The Vector TrueVIEW Autofluorescence Quenching Kit provides a dramatic reduction of autofluorescence, allowing a clear view of the desired signal, so it was utilized as needed on the specimens represented in the images. 

If co-labeling with a primary antibody, users should optimize each protocol separately to achieve maximum signal to noise ratios. Once this is complete, the two protocols can be combined, with the Glysite Scout Glycan Screening Kit protocol applied first, followed by the antibody staining protocol. Users should perform the proper controls to demonstrate that the reagents in each protocol are not cross–reacting with each other. 

The Carbo-Free Blocking Solution included in the Glysite Scout Glycan Screening Kit is virtually free of glycoproteins or other glycosylated molecules. This is critical since these kits detect glycans and would therefore bind to blocking reagents which contained glycosylated components. Users should not use other blocking solutions (e.g., milk derivatives, sera) as it may lead to high background. 

Each Glysite Scout Glycan Screening Kit contains enough reagents for approximately 50 tissue samples per lectin, or up to 400 samples if using one lectin per tissue sample (8 lectins x 50 tissue samples). 

The lectins were selected based upon their broad applicability and specificity to frequently cited glycan targets from literature sources, spanning fields of Cancer, Immunology, Metabolism, Inflammation, Virology, and Tumor Microenvironment, among others. They enable detection of all the major glycan motifs for the evaluation of glycan distribution in a target specimen. 

Technical Information

The lectins selected for the Glysite Scout Glycan Screening Kit, Immunofluorescence 488 were specifically chosen to provide detection of all of the major glycan motifs for the evaluation of glycan distribution in a target specimen. Follow our optimized protocol or view our how-to video to learn how you can rapidly explore glycan expression in your tissue section.

In addition to the glycan binders, the kit includes optimized immunofluorescence reagents for glycan detection, including blocking solutions, DyLight™ streptavidin, and VECTASHIELD Vibrance® Antifade Mounting Medium with DAPI.

Legal Information

DyLight is a trademark of Pierce Biotechnology, Inc.

The post Glysite™ Scout Glycan Screening Kit, Immunofluorescence 488 appeared first on VectorLabs.

]]> Glysite™ Scout Glycan Screening Kit, Immunofluorescence 594 https://staging.vectorlabs.com/products/glysite-scout-screening-kit-594/ Sun, 30 Apr 2023 03:28:46 +0000 https://staging.vectorlabs.com/?post_type=product&p=13436 Description

Glysite Scout Glycan Screening Kit, Immunofluorescence 594 is a fully integrated kit for the detection of glycan expression in tissue sections. Included in the kit is a diverse panel of glycan binders (lectins) ideal for the preliminary screening of tissue samples with unknown glycosylation changes with specificity to mannose, complex N-glycan, core O-glycan, fucose, sialic acid, sulfation, GlcNAc, chitin, galactose, and LacNAc.

Validated on human and mouse tissue—including FFPE tissue, across a range of tissue types including colon, lung, spleen, kidney, liver, pancreas, testes, heart, bladder, and uterus—this versatile kit is flexible to meet your needs. Glysite Scout Glycan Screening Kits, Immunofluorescence are available with 3 fluorophore options, 488 (GSK-3000), 594 (GSK-2000), and 649 (GSK-1000).

Specifications

Unit Size 1 kit
Applications Immunofluorescence, Glycobiology
Recommended Storage Store reagents in original bottles at 2–8 °C. Do not freeze the kit.
Conjugate DyLight 549
Sugar Specificity Mannose, Galactose, Fucose, Sialic Acid

 

Kit Components

Kit Contents:

This glycan screening kit is designed to provide the investigator with a panel of eight biotinylated lectin conjugates. The lectins have been selected to offer a variety of sugar specificities and are of the same high quality as the reagents offered individually.

Glysite Scout Glycan Screening Kit, Immunofluorescence 594 contains the following biotinylated lectins:

  • AAL (Aleuria Aurantia)
  • ECL, ECA (Erythrina Cristagalli)
  • GNL (Galanthus Nivalis)
  • Jacalin
  • MAL II (Maackia Amurensis II)
  • PHA-L (Phaseolus Vulgaris Leucoagglutinin)
  • WFA, WFL (Wisteria Fluoribunda)
  • WGA (Wheat Germ Agglutinin)

In addition to the glycan binders, the kit includes optimized immunofluorescence reagents for glycan detection, including blocking solutions, DyLight™ streptavidin, and VECTASHIELD Vibrance® Antifade Mounting Medium with DAPI.

The post Glysite™ Scout Glycan Screening Kit, Immunofluorescence 594 appeared first on VectorLabs.

]]>

Resources

Description

Glysite Scout Glycan Screening Kit, Immunofluorescence 594 is a fully integrated kit for the detection of glycan expression in tissue sections. Included in the kit is a diverse panel of glycan binders (lectins) ideal for the preliminary screening of tissue samples with unknown glycosylation changes with specificity to mannose, complex N-glycan, core O-glycan, fucose, sialic acid, sulfation, GlcNAc, chitin, galactose, and LacNAc.

Validated on human and mouse tissue—including FFPE tissue, across a range of tissue types including colon, lung, spleen, kidney, liver, pancreas, testes, heart, bladder, and uterus—this versatile kit is flexible to meet your needs. Glysite Scout Glycan Screening Kits, Immunofluorescence are available with 3 fluorophore options, 488 (GSK-3000), 594 (GSK-2000), and 649 (GSK-1000).

Specifications

Unit Size1 kit
ApplicationsImmunofluorescence, Glycobiology
Recommended StorageStore reagents in original bottles at 2–8 °C. Do not freeze the kit.
ConjugateDyLight 549
Sugar SpecificityMannose, Galactose, Fucose, Sialic Acid

Kit Components

Kit Contents:

This glycan screening kit is designed to provide the investigator with a panel of eight biotinylated lectin conjugates. The lectins have been selected to offer a variety of sugar specificities and are of the same high quality as the reagents offered individually.

Glysite Scout Glycan Screening Kit, Immunofluorescence 594 contains the following biotinylated lectins:

  • AAL (Aleuria Aurantia)
  • ECL, ECA (Erythrina Cristagalli)
  • GNL (Galanthus Nivalis)
  • Jacalin
  • MAL II (Maackia Amurensis II)
  • PHA-L (Phaseolus Vulgaris Leucoagglutinin)
  • WFA, WFL (Wisteria Fluoribunda)
  • WGA (Wheat Germ Agglutinin)

In addition to the glycan binders, the kit includes optimized immunofluorescence reagents for glycan detection, including blocking solutions, DyLight™ streptavidin, and VECTASHIELD Vibrance® Antifade Mounting Medium with DAPI. 

Documents

Product FAQs

Virtually any tissue type and species are suitable for use with a Glysite Scout Glycan Screening Kit, Immunofluorescence, as glycans are ubiquitous in nature and in life. The kit has been validated on human and mouse tissue—including FFPE tissue, across a range of organs, including colon, lung, spleen, kidney, liver, pancreas, testes, heart, bladder, and uterus.

Each of the three Glysite Scout Glycan Screening Kits, Immunofluorescence contain the same lectins but with a different fluorescent streptavidin detection reagent, offering a choice of Dylight™ 488 (GSK-3000), Dylight™ 594 (GSK-2000), or Dylight™ 649 (GSK-1000). This allows users to choose the kit which best matches the capabilities of their fluorescent microscope and also provides flexibility for multiplexing with other fluorescent detection reagents they might already be using. 

VECTASHIELD Vibrance is an antifade medium for immunofluorescence applications with setting properties, which means the coverslip is immobilized after curing and does not require sealing. Additionally, slides can be stored at room temperature, enabling extended archiving time. VECTASHIELD PLUS does not have setting properties and remains molten, and slides must be stored at 2–8°C. Both VECTASHIELD Vibrance and VECTASHIELD PLUS have superior antifade/anti-photobleaching properties across the spectrum and are compatible with commonly used fluorophores. 

The need for an antigen retrieval step in a staining protocol depends on multiple variables, including the target glycan and its location in the specimen, the glycan-binder, the type of tissue, and the method and length of fixation. Investigators should run controls with and without antigen retrieval to determine if this step is needed for their specimen. 

Tissue components such as red blood cells, elastin, and collagen are strongly fluorescent, particularly in FFPE sections, making it difficult to discern between relevant signal and background. Formalin fixation tends to induce a significant amount of fluorescence to a specimen. The Vector TrueVIEW Autofluorescence Quenching Kit provides a dramatic reduction of autofluorescence, allowing a clear view of the desired signal, so it was utilized as needed on the specimens represented in the images. 

If co-labeling with a primary antibody, users should optimize each protocol separately to achieve maximum signal to noise ratios. Once this is complete, the two protocols can be combined, with the Glysite Scout Glycan Screening Kit protocol applied first, followed by the antibody staining protocol. Users should perform the proper controls to demonstrate that the reagents in each protocol are not cross–reacting with each other. 

The Carbo-Free Blocking Solution included in the Glysite Scout Glycan Screening Kit is virtually free of glycoproteins or other glycosylated molecules. This is critical since these kits detect glycans and would therefore bind to blocking reagents which contained glycosylated components. Users should not use other blocking solutions (e.g., milk derivatives, sera) as it may lead to high background. 

Each Glysite Scout Glycan Screening Kit contains enough reagents for approximately 50 tissue samples per lectin, or up to 400 samples if using one lectin per tissue sample (8 lectins x 50 tissue samples). 

The lectins were selected based upon their broad applicability and specificity to frequently cited glycan targets from literature sources, spanning fields of Cancer, Immunology, Metabolism, Inflammation, Virology, and Tumor Microenvironment, among others. They enable detection of all the major glycan motifs for the evaluation of glycan distribution in a target specimen. 

Technical Information

The lectins selected for the Glysite Scout Glycan Screening Kit, Immunofluorescence 594 were specifically chosen to provide detection of all of the major glycan motifs for the evaluation of glycan distribution in a target specimen. Follow our optimized protocol or view our how-to video to learn how you can rapidly explore glycan expression in your tissue section.

In addition to the glycan binders, the kit includes optimized immunofluorescence reagents for glycan detection, including blocking solutions, DyLight™ streptavidin, and VECTASHIELD Vibrance® Antifade Mounting Medium with DAPI. 

Legal Information

DyLight is a trademark of Pierce Biotechnology, Inc.

The post Glysite™ Scout Glycan Screening Kit, Immunofluorescence 594 appeared first on VectorLabs.

]]> Glysite™ Scout Glycan Screening Kit, Immunofluorescence 649 https://staging.vectorlabs.com/products/glysite-scout-screening-kit-649/ Sun, 30 Apr 2023 03:21:25 +0000 https://staging.vectorlabs.com/?post_type=product&p=13428 Description

Glysite Scout Glycan Screening Kit, Immunofluorescence 649 is a fully integrated kit for the detection of glycan expression in tissue sections. Included in the kit is a diverse panel of glycan binders (lectins) ideal for the preliminary screening of tissue samples with unknown glycosylation changes with specificity to mannose, complex N-glycan, core O-glycan, fucose, sialic acid, sulfation, GlcNAc, chitin, galactose, and LacNAc.

Validated on human and mouse tissue—including FFPE tissue, across a range of tissue types including colon, lung, spleen, kidney, liver, pancreas, testes, heart, bladder, and uterus—this versatile kit is flexible to meet your needs. Glysite Scout Glycan Screening Kits, Immunofluorescence are available with 3 fluorophore options, 488 (GSK-3000), 594 (GSK-2000), and 649 (GSK-1000).

Specifications

Unit Size 1 kit
Applications Immunofluorescence, Glycobiology
Recommended Storage Store reagents in original bottles at 2–8 °C. Do not freeze the kit.
Conjugate DyLight 649
Sugar Specificity Mannose, Galactose, Fucose, Sialic Acid

Kit Components

Kit Contents:

This glycan screening kit is designed to provide the investigator with a panel of eight biotinylated lectin conjugates. The lectins have been selected to offer a variety of sugar specificities and are of the same high quality as the reagents offered individually.

Glysite Scout Glycan Screening Kit, Immunofluorescence 649 contains the following biotinylated lectins:

  • AAL (Aleuria Aurantia)
  • ECL, ECA (Erythrina Cristagalli)
  • GNL (Galanthus Nivalis)
  • Jacalin
  • MAL II (Maackia Amurensis II)
  • PHA-L (Phaseolus Vulgaris Leucoagglutinin)
  • WFA, WFL (Wisteria Fluoribunda)
  • WGA (Wheat Germ Agglutinin)

 

In addition to the glycan binders, the kit includes optimized immunofluorescence reagents for glycan detection, including blocking solutions, DyLight™ streptavidin, and VECTASHIELD Vibrance® Antifade Mounting Medium with DAPI.

The post Glysite™ Scout Glycan Screening Kit, Immunofluorescence 649 appeared first on VectorLabs.

]]>

Description

Glysite Scout Glycan Screening Kit, Immunofluorescence 649 is a fully integrated kit for the detection of glycan expression in tissue sections. Included in the kit is a diverse panel of glycan binders (lectins) ideal for the preliminary screening of tissue samples with unknown glycosylation changes with specificity to mannose, complex N-glycan, core O-glycan, fucose, sialic acid, sulfation, GlcNAc, chitin, galactose, and LacNAc.

Validated on human and mouse tissue—including FFPE tissue, across a range of tissue types including colon, lung, spleen, kidney, liver, pancreas, testes, heart, bladder, and uterus—this versatile kit is flexible to meet your needs. Glysite Scout Glycan Screening Kits, Immunofluorescence are available with 3 fluorophore options, 488 (GSK-3000), 594 (GSK-2000), and 649 (GSK-1000).

Specifications

Unit Size1 kit
ApplicationsImmunofluorescence, Glycobiology
Recommended StorageStore reagents in original bottles at 2–8 °C. Do not freeze the kit.
ConjugateDyLight 649
Sugar SpecificityMannose, Galactose, Fucose, Sialic Acid

Kit Components

Kit Contents:

This glycan screening kit is designed to provide the investigator with a panel of eight biotinylated lectin conjugates. The lectins have been selected to offer a variety of sugar specificities and are of the same high quality as the reagents offered individually.

Glysite Scout Glycan Screening Kit, Immunofluorescence 649 contains the following biotinylated lectins:

  • AAL (Aleuria Aurantia)
  • ECL, ECA (Erythrina Cristagalli)
  • GNL (Galanthus Nivalis)
  • Jacalin
  • MAL II (Maackia Amurensis II)
  • PHA-L (Phaseolus Vulgaris Leucoagglutinin)
  • WFA, WFL (Wisteria Fluoribunda)
  • WGA (Wheat Germ Agglutinin)

In addition to the glycan binders, the kit includes optimized immunofluorescence reagents for glycan detection, including blocking solutions, DyLight™ streptavidin, and VECTASHIELD Vibrance® Antifade Mounting Medium with DAPI. 

Documents

Product FAQs

Virtually any tissue type and species are suitable for use with a Glysite Scout Glycan Screening Kit, Immunofluorescence, as glycans are ubiquitous in nature and in life. The kit has been validated on human and mouse tissue—including FFPE tissue, across a range of organs, including colon, lung, spleen, kidney, liver, pancreas, testes, heart, bladder, and uterus.

Each of the three Glysite Scout Glycan Screening Kits, Immunofluorescence contain the same lectins but with a different fluorescent streptavidin detection reagent, offering a choice of Dylight™ 488 (GSK-3000), Dylight™ 594 (GSK-2000), or Dylight™ 649 (GSK-1000). This allows users to choose the kit which best matches the capabilities of their fluorescent microscope and also provides flexibility for multiplexing with other fluorescent detection reagents they might already be using. 

VECTASHIELD Vibrance is an antifade medium for immunofluorescence applications with setting properties, which means the coverslip is immobilized after curing and does not require sealing. Additionally, slides can be stored at room temperature, enabling extended archiving time. VECTASHIELD PLUS does not have setting properties and remains molten, and slides must be stored at 2–8°C. Both VECTASHIELD Vibrance and VECTASHIELD PLUS have superior antifade/anti-photobleaching properties across the spectrum and are compatible with commonly used fluorophores. 

The need for an antigen retrieval step in a staining protocol depends on multiple variables, including the target glycan and its location in the specimen, the glycan-binder, the type of tissue, and the method and length of fixation. Investigators should run controls with and without antigen retrieval to determine if this step is needed for their specimen. 

Tissue components such as red blood cells, elastin, and collagen are strongly fluorescent, particularly in FFPE sections, making it difficult to discern between relevant signal and background. Formalin fixation tends to induce a significant amount of fluorescence to a specimen. The Vector TrueVIEW Autofluorescence Quenching Kit provides a dramatic reduction of autofluorescence, allowing a clear view of the desired signal, so it was utilized as needed on the specimens represented in the images. 

If co-labeling with a primary antibody, users should optimize each protocol separately to achieve maximum signal to noise ratios. Once this is complete, the two protocols can be combined, with the Glysite Scout Glycan Screening Kit protocol applied first, followed by the antibody staining protocol. Users should perform the proper controls to demonstrate that the reagents in each protocol are not cross–reacting with each other. 

The Carbo-Free Blocking Solution included in the Glysite Scout Glycan Screening Kit is virtually free of glycoproteins or other glycosylated molecules. This is critical since these kits detect glycans and would therefore bind to blocking reagents which contained glycosylated components. Users should not use other blocking solutions (e.g., milk derivatives, sera) as it may lead to high background. 

Each Glysite Scout Glycan Screening Kit contains enough reagents for approximately 50 tissue samples per lectin, or up to 400 samples if using one lectin per tissue sample (8 lectins x 50 tissue samples). 

The lectins were selected based upon their broad applicability and specificity to frequently cited glycan targets from literature sources, spanning fields of Cancer, Immunology, Metabolism, Inflammation, Virology, and Tumor Microenvironment, among others. They enable detection of all the major glycan motifs for the evaluation of glycan distribution in a target specimen. 

Technical Information

The lectins selected for the Glysite Scout Glycan Screening Kit, Immunofluorescence 649 were specifically chosen to provide detection of all of the major glycan motifs for the evaluation of glycan distribution in a target specimen. Follow our optimized protocol or view our how-to video to learn how you can rapidly explore glycan expression in your tissue section.

In addition to the glycan binders, the kit includes optimized immunofluorescence reagents for glycan detection, including blocking solutions, DyLight™ streptavidin, and VECTASHIELD Vibrance® Antifade Mounting Medium with DAPI.

Legal Information

DyLight is a trademark of Pierce Biotechnology, Inc.

The post Glysite™ Scout Glycan Screening Kit, Immunofluorescence 649 appeared first on VectorLabs.

]]> Lectin Kit II, Biotinylated https://staging.vectorlabs.com/products/lectin-kit-2-biotinylated/ Sat, 29 Apr 2023 18:26:36 +0000 https://staging.vectorlabs.com/?post_type=product&p=13372

Description

Our lectin screening kits are designed to provide the investigator with a panel of six lectins or lectin conjugates. The lectins have been selected to offer a variety of sugar specificities and are of the same high quality as the reagents offered individually.

Each Kit II (LK-3000, BK-2100, FLK-3100, RLK-3200) consists of 1 mg of the following lectins or lectin conjugates: GSL I, LCA, PHA-E, PHA-L, PSA, Succinylated WGA.

Specifications

Unit Size 1 kit
Applications Immunohistochemistry / Immunocytochemistry, Immunofluorescence, Blotting Applications, Elispot, ELISAs, Glycobiology
Conjugate Biotinylated

Kit Contents:

This lectin screening kit is designed to provide the investigator with a panel of six biotinylated lectin conjugates. The lectins have been selected to offer a variety of sugar specificities and are of the same high quality as the reagents offered individually.

Kit II consists of 1 mg of the following biotinylated lectins:

  • GSL I (Griffonia (Bandeiraea) simplicifolia lectin I; 2 mg/ml)
  • PSA (Pisum sativum agglutinin; 2 mg/ml)
  • LCA (Lens culinaris agglutinin; 2 mg/ml)
  • PHA-E (Phaseolus vulgaris Erythroagglutinin; 2 mg/ml)
  • PHA-L (Phaseolus vulgaris Leucoagglutinin; 2 mg/ml)
  • Succinylated WGA (Wheat germ agglutinin, succinylated; 2 mg/ml)

The post Lectin Kit II, Biotinylated appeared first on VectorLabs.

]]>

Resources

Description

Our lectin screening kits are designed to provide the investigator with a panel of six lectins or lectin conjugates. The lectins have been selected to offer a variety of sugar specificities and are of the same high quality as the reagents offered individually.

Each Kit II (LK-3000, BK-2100, FLK-3100, RLK-3200) consists of 1 mg of the following lectins or lectin conjugates: GSL I, LCA, PHA-E, PHA-L, PSA, Succinylated WGA.

Specifications

Unit Size1 kit
ApplicationsImmunohistochemistry / Immunocytochemistry, Immunofluorescence, Blotting Applications, Elispot, ELISAs, Glycobiology
ConjugateBiotinylated

Kit Contents:

This lectin screening kit is designed to provide the investigator with a panel of six biotinylated lectin conjugates. The lectins have been selected to offer a variety of sugar specificities and are of the same high quality as the reagents offered individually.

Kit II consists of 1 mg of the following biotinylated lectins:

  • GSL I (Griffonia (Bandeiraea) simplicifolia lectin I; 2 mg/ml)
  • PSA (Pisum sativum agglutinin; 2 mg/ml)
  • LCA (Lens culinaris agglutinin; 2 mg/ml)
  • PHA-E (Phaseolus vulgaris Erythroagglutinin; 2 mg/ml)
  • PHA-L (Phaseolus vulgaris Leucoagglutinin; 2 mg/ml)
  • Succinylated WGA (Wheat germ agglutinin, succinylated; 2 mg/ml)

Documents

Technical Information

These bioinylated conjugates are prepared from our affinity-purified lectins and are optimally labeled with biotin. Essentially free of inactive lectin conjugates and containing no free biotin, these biotinylated lectins provide ideal intermediates for examining glycoconjugates using the Biotin-Avidin/Streptavidin System. First a biotin-labeled lectin is added, followed by the VECTASTAIN® ABC Reagent, Avidin D conjugate, or streptavidin derivative.

The post Lectin Kit II, Biotinylated appeared first on VectorLabs.

]]> Concanavalin A (Con A), Rhodamine https://staging.vectorlabs.com/products/rhodamine-concanavalin-a-con-a/ Fri, 28 Apr 2023 19:55:21 +0000 https://staging.vectorlabs.com/?post_type=product&p=12848 Description

Con A recognizes α-linked mannose present as part of a “core oligosaccharide” in many serum and membrane glycoproteins.

Rhodamine labeled Con A has an appropriate number of fluorochromes bound to provide the optimum staining characteristics for this lectin. This conjugate is supplied essentially free of unconjugated fluorochromes. The excitation maximum is at 550 nm and the emission maximum is at 575 nm.

Specifications

Unit Size 25 mg
Applications Immunofluorescence, Glycobiology
Recommended Usage The recommended concentration range for use is 5-20 µg/ml.
Recommended Storage 2-8 °C
Maximum Excitation 545-555 nm
Maximum Emission 570-580 nm
Solution 10 mM HEPES, 0.15 M NaCl, pH 7.5 0.08% sodium azide, 0.1 mM CaCl2, 0.01 mM MnCl2, 5 mM mannose
Concentration 5 mg active conjugate/ml
Conjugate Rhodamine
Color of Fluorescence Red
Sugar Specificity Mannose, Glucose

The post Concanavalin A (Con A), Rhodamine appeared first on VectorLabs.

]]>

Description

Con A recognizes α-linked mannose present as part of a “core oligosaccharide” in many serum and membrane glycoproteins.

Rhodamine labeled Con A has an appropriate number of fluorochromes bound to provide the optimum staining characteristics for this lectin. This conjugate is supplied essentially free of unconjugated fluorochromes. The excitation maximum is at 550 nm and the emission maximum is at 575 nm.

Specifications

Unit Size25 mg
ApplicationsImmunofluorescence, Glycobiology
Recommended UsageThe recommended concentration range for use is 5-20 µg/ml.
Recommended Storage2-8 °C
Maximum Excitation545-555 nm
Maximum Emission570-580 nm
Solution10 mM HEPES, 0.15 M NaCl, pH 7.5 0.08% sodium azide, 0.1 mM CaCl2, 0.01 mM MnCl2, 5 mM mannose
Concentration5 mg active conjugate/ml
ConjugateRhodamine
Color of FluorescenceRed
Sugar SpecificityMannose, Glucose

Documents

Citations

Technical Information

At neutral and alkaline pH, Con A exists as a tetramer of four identical subunits; below pH 5.6, Con A dissociates into active dimers of 52 kDa. Acetylation, succinylation, or other derivatizations can also produce stable forms with dimeric structures. (See succinylated Con A). Nicks in the sequence are often present in the purest preparations due to hydrolytic damage within the seeds.

Con A requires calcium or manganese ions at each of its four saccharide binding sites. Although these divalent metal ions are bound tightly to the polypeptide structure, buffers which can bind calcium (such as phosphate) generally should be avoided in diluting Con A, since a gradual loss in activity may occur.

Accompanying each fluorescent lectin is an analysis data sheet summarizing the results of our quality control tests and providing pertinent information on the product. All of these reagents are supplied as solutions preserved with sodium azide.

Inhibiting/Eluting Sugar: mixture of 200 mM α-methylmannoside/200 mM α-methylglucoside

The post Concanavalin A (Con A), Rhodamine appeared first on VectorLabs.

]]> Narcissus Pseudonarcissus (Daffodil) Lectin (NPL, NPA), Unconjugated https://staging.vectorlabs.com/products/unconj-narcissus-pseudonarcissus-lectin/ Thu, 27 Apr 2023 17:25:42 +0000 https://staging.vectorlabs.com/?post_type=product&p=12511 Description

NPL, isolated from daffodil bulbs, has a specificity toward α-linked mannose, preferring polymannose structures containing (α-1,6) linkages. Binding to mannose polymers can occur via internal mannose residues and is not dependent on structural integrity of a non-reducing end sugar. NPL also binds some galactomannans, and differs in other binding characteristics from a related lectin, Galanthus nivalis lectin. Unlike Con A, LCA, or PSA, NPL does not bind glucose.

Specifications

Unit Size 5 mg
Applications Glycobiology
Recommended Usage Although many buffers can be employed for reconstituting and diluting this lectin, 10 mM HEPES buffered saline, pH 8.5, 0.1 mM CaCl2 is recommended. For preserving solutions stored at 4 ºC, 0.08% sodium azide can be used.
Recommended Storage 2-8 °C
Conjugate Unconjugated
Sugar Specificity Mannose

The post Narcissus Pseudonarcissus (Daffodil) Lectin (NPL, NPA), Unconjugated appeared first on VectorLabs.

]]>

Description

NPL, isolated from daffodil bulbs, has a specificity toward α-linked mannose, preferring polymannose structures containing (α-1,6) linkages. Binding to mannose polymers can occur via internal mannose residues and is not dependent on structural integrity of a non-reducing end sugar. NPL also binds some galactomannans, and differs in other binding characteristics from a related lectin, Galanthus nivalis lectin. Unlike Con A, LCA, or PSA, NPL does not bind glucose.

Specifications

Unit Size5 mg
ApplicationsGlycobiology
Recommended UsageAlthough many buffers can be employed for reconstituting and diluting this lectin, 10 mM HEPES buffered saline, pH 8.5, 0.1 mM CaCl2 is recommended. For preserving solutions stored at 4 ºC, 0.08% sodium azide can be used.
Recommended Storage2-8 °C
ConjugateUnconjugated
Sugar SpecificityMannose

Documents

Technical Information

This lectin exists as a tetramer at neutral pH but below pH 5.0 and above pH 9.0, NPL dissociates into monomers. 

Inhibiting Sugar: 400 mM α-methylmannoside

The post Narcissus Pseudonarcissus (Daffodil) Lectin (NPL, NPA), Unconjugated appeared first on VectorLabs.

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