Horse – VectorLabs https://staging.vectorlabs.com From linker design and synthesis, bioconjugated design and manufacturing, biomolecule labeling and functionalization, and detection system and solutions, our team is here to help you move forward, with impact. Wed, 22 Oct 2025 17:15:30 +0000 en-US hourly 1 https://wordpress.org/?v=6.9 https://staging.vectorlabs.com/wp-content/uploads/2024/11/V-favicon-02-100x100.png Horse – VectorLabs https://staging.vectorlabs.com 32 32 VECTASTAIN® Elite® ABC Universal PLUS Kit, Peroxidase (Horse Anti-Mouse/Rabbit IgG) https://staging.vectorlabs.com/products/vectastain-elite-hrp-horse-anti-mouse-rabbit-igg/ Tue, 02 May 2023 20:43:16 +0000 https://staging.vectorlabs.com/?post_type=product&p=14741 Description

The VECTASTAIN Elite ABC system is the most sensitive avidin/biotin-based peroxidase system and is approximately 5 times more sensitive than the original VECTASTAIN ABC Kit with the same low background. Our Universal Biotinylated Anti-Mouse/Rabbit IgG secondary antibody included in this kit is a cocktail of biotinylated anti-mouse IgG and anti-rabbit IgG, designed for use with both rabbit and mouse primary antibodies. This antibody should not be used to stain tissues from rodents or rabbits due to its reactivity with IgG present in the tissues of these species.

Features:

  • Advanced avidin/biotin technology: The Elite ABC complex is smaller, very uniform, and highly active, allowing more accessibility for binding to a biotinylated target
  • Cost effective: Higher sensitivity leads to lower cost per slide
  • More complete detection systems: Inclusion of BLOXALL® endogenous enzyme quenching solution and ImmPACT® DAB EqV substrate in the PLUS kit provides added convenience with optimized, matched reagents in the one product.

Specifications

Unit Size 1 Kit
Applications Immunohistochemistry / Immunocytochemistry, In situ hybridization, Blotting Applications, Elispot, ELISAs
Technology ABC Elite (Avidin/Biotin) Systems
Detection Enzyme(s) Peroxidase
Target Species Universal (Mouse/Rabbit)
Format Ready-to-Use

Kit Contents:

  • 50 ml ready-to-use stabilized Elite ABC reagent
  • 50 ml ready-to-use universal, biotinylated anti-mouse/rabbit IgG
  • 50 ml 2.5% normal horse serum
  • 50 ml BLOXALL Endogenous Enzyme Blocking Solution
  • ImmPACT DAB EqV Substrate (25 ml Reagent 1 + 25 ml Reagent 2)

The post VECTASTAIN® Elite® ABC Universal PLUS Kit, Peroxidase (Horse Anti-Mouse/Rabbit IgG) appeared first on VectorLabs.

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Description

The VECTASTAIN Elite ABC system is the most sensitive avidin/biotin-based peroxidase system and is approximately 5 times more sensitive than the original VECTASTAIN ABC Kit with the same low background. Our Universal Biotinylated Anti-Mouse/Rabbit IgG secondary antibody included in this kit is a cocktail of biotinylated anti-mouse IgG and anti-rabbit IgG, designed for use with both rabbit and mouse primary antibodies. This antibody should not be used to stain tissues from rodents or rabbits due to its reactivity with IgG present in the tissues of these species.

Features:

  • Advanced avidin/biotin technology: The Elite ABC complex is smaller, very uniform, and highly active, allowing more accessibility for binding to a biotinylated target
  • Cost effective: Higher sensitivity leads to lower cost per slide
  • More complete detection systems: Inclusion of BLOXALL® endogenous enzyme quenching solution and ImmPACT® DAB EqV substrate in the PLUS kit provides added convenience with optimized, matched reagents in the one product.

Specifications

Unit Size1 Kit
ApplicationsImmunohistochemistry / Immunocytochemistry, In situ hybridization, Blotting Applications, Elispot, ELISAs
TechnologyABC Elite (Avidin/Biotin) Systems
Detection Enzyme(s)Peroxidase
Target SpeciesUniversal (Mouse/Rabbit)
FormatReady-to-Use

Kit Contents:

  • 50 ml ready-to-use stabilized Elite ABC reagent
  • 50 ml ready-to-use universal, biotinylated anti-mouse/rabbit IgG
  • 50 ml 2.5% normal horse serum
  • 50 ml BLOXALL Endogenous Enzyme Blocking Solution
  • ImmPACT DAB EqV Substrate (25 ml Reagent 1 + 25 ml Reagent 2)

Documents

Product FAQs

We offer a choice of either peroxidase or alkaline phosphatase based VECTASTAIN ABC kit detection systems. Selection of which enzyme system to use would be the first step. Most IHC applications involve detecting an unconjugated primary antibody. Consider the species in which the primary antibody is raised (e.g. rabbit), and then match to to corresponding species-specific VECTASTAIN ABC kit (e.g. VECTASTAIN ABC Rabbit IgG kit). If you already have a biotinylated target (i.e. biotinylated secondary antibody or primary antibody), then a standard VECTASTAIN ABC kit containing just the avidin/biotinylated enzyme reagents would be required. Note that only the VECTASTAIN Universal Elite PLUS ABC kit (PK-8200) contains a substrate.

Citations

Technical Information

With more than 50,000 citations to its credit, the VECTASTAIN ABC kit remains widely popular. Based on the versatile avidin-biotin complex interaction, the system is modular, and along with our selection of secondary antibodies, can accommodate a wide array of primary antibody and tissue species. Our ABC kits are economical and continue to be a staple product in any immunohistochemistry (IHC) and immunocytochemistry (ICC) laboratory.

Peroxidase substrates produce sharp, dense precipitates with crisp localization. These characteristics, in conjunction with the high sensitivity and low background of the VECTASTAIN ABC systems, make the peroxidase enzyme a preferred choice in many applications. (eg. In neural tissue, the peroxidase system is often preferred because it gives more consistent labeling of both cell bodies and processes.)

VECTASTAIN Elite ABC Kit Components:

  • 50 ml ready-to-use stabilized Elite® ABC reagent
  • 50 ml ready-to-use universal, biotinylated anti-mouse/rabbit IgG
  • 50 ml 2.5% normal horse serum
  • 50 ml BLOXALL Endogenous Enzyme Blocking Solution
  • ImmPACT DAB EqV Substrate (25 ml Reagent 1 + 25 ml Reagent 2)

Sufficient reagents to stain approximately 250-500 sections.

The Avidin Biotin Complex Method

The VECTASTAIN ABC systems are extremely sensitive due to the form and number of active enzyme molecules associated with the preformed Avidin/Biotinylated enzyme Complex. This ABC complex takes advantage of two important properties of avidin: 1) an extraordinarily high affinity for biotin (over one million times higher than antibody for most antigens), and 2) four biotin-binding sites. These properties allow macromolecular complexes (ABCs) to be formed by mixing Avidin DH (Reagent A) with its paired biotinylated enzyme (Reagent B) prior to use. The ABC reagent, once formed, remains stable for many hours after formation and can be used for several days after preparation.

Biotinylated primary antibodies, secondaries, lectins, neuronal tracers, nucleic acids, and ligands can be effectively visualized in applications such as:

  • Tissue staining
  • Multiple labeling (Multiplex IHC)
  • Western blotting
  • Southern and northern blotting
  • In situ hybridization detection (ISH)
  • Enzyme immunoassays (ELISA)
  • Neuronal tracing

All applications benefit from the high sensitivity, low background, reproducibility, and economy of the VECTASTAIN ABC system.

ABCuse 1 jpg

Choosing a VECTASTAIN ABC Kit

1) Choose an enzyme system:

The VECTASTAIN ABC Kits are available with a choice of two different enzyme systems

  • Peroxidase (ABC, Elite® ABC, RTU or Kits)
  • Alkaline Phosphatase (ABC-AP)

 

2) Choose the biotinylated secondary antibody:

To detect a biotinylated target, you will need only the ABC Reagent contained in the Standard Kit, followed by an appropriate substrate.

To detect an unlabeled primary antibody, you will need a biotinylated secondary antibody that binds to the primary antibody species chosen, the ABC Reagent, and an appropriate substrate. For example, to detect a primary antibody made in rabbit, the appropriate choice is a VECTASTAIN ABC Kit designated “Rabbit IgG”.

For additional versatility and convenience, Vector Laboratories offers secondary antibody products that recognize more than one species of primary antibody. Our Universal Biotinylated Anti-Mouse/Rabbit IgG secondary antibody (Cat. No. BA-1400) is a cocktail of biotinylated anti-mouse IgG and anti-rabbit IgG, designed for use with both rabbit and mouse primary antibodies. This antibody should not be used to stain tissues from rodents or rabbits due to its reactivity with IgG present in the tissues of these species. The Universal Pan-Specific Anti- Mouse/Rabbit/Goat secondary antibody (Cat. No. BA-1300) (which is included in our VECTASTAIN Universal Kits) is a cocktail of biotinylated anti-mouse IgG, anti-rabbit IgG, and anti-goat IgG that recognizes mouse, rabbit, and goat primary antibodies. This secondary antibody should not be used to stain rat, mouse, or other rodent, rabbit, goat, bovine, or sheep tissue due to potential reactivity with endogenous IgG.

3) Choose a convenient format:

Finally, several kits are available in prediluted, stabilized formats for convenience and ease of use. See kits designated R.T.U. or Ready-To-Use.

Consider Species Cross-Reactivity

When choosing the optimal detection system for your application, it is important to consider not only the species of the primary antibody but also the species of the tissue under examination. If the species of the primary antibody and the species of the tissue are closely related (e.g. rat and mouse), the biotinylated secondary antibody may cross react with endogenous IgG in the tissue section leading to background staining. The following three options minimize background staining in these instances:

  • Biotinylate the primary antibody and detect with a Standard VECTASTAIN Elite ABC Kit, PK-6100 (only includes the avidin-biotin complex, or ABC reagent).
  • Use a biotinylated secondary antibody specifically adsorbed to remove cross-reacting antibodies of closely-related species (e.g. biotinylated anti-mouse IgG, rat adsorbed, Cat. No. BA-2001).
  • Use the M.O.M.® Immunodetection System for applications of mouse primary antibodies on mouse tissue.

 

Substrates

After choosing the VECTASTAIN ABC Kit for your application, select a substrate that matches the enzyme system of the kit.

The post VECTASTAIN® Elite® ABC Universal PLUS Kit, Peroxidase (Horse Anti-Mouse/Rabbit IgG) appeared first on VectorLabs.

]]> ImmPRESS® HRP Horse Anti-Mouse IgG PLUS Polymer Kit, Peroxidase https://staging.vectorlabs.com/products/immpress-hrp-horse-anti-mouse-igg-plus-kit/ Tue, 02 May 2023 20:35:50 +0000 https://staging.vectorlabs.com/?post_type=product&p=14729 Description

The ImmPRESS polymerized reporter enzyme staining system uses novel conjugation and micropolymer chemistries to create a highly sensitive, ready-to-use, one-step detection system. This unique micropolymer of highly active enzyme (peroxidase) is attached to our affinity purified secondary antibodies, producing reagents with outstanding sensitivity and low background. The inclusion of BLOXALL® endogenous enzyme quenching solution and ImmPACT® DAB EqV substrate in the PLUS kit provides a more complete detection system.

Features:

  • Enhanced accessibility to nuclear and membrane antigens
  • Ready-to-use in a convenient dropper bottle
  • Shorter assay time
  • Simplified multiple labeling

 

The ImmPRESS (15 ml) Kit will stain approximately 75-150 sections.

Specifications

Unit Size 1 Kit
Applications Immunohistochemistry / Immunocytochemistry, In situ hybridization, ELISAs
Technology Micropolymer Reagents
Detection Enzyme(s) Peroxidase
Conjugate Micropolymer HRP
Format Ready-to-Use

Kit Contents:

  • 15 ml ImmPRESS (Peroxidase) Polymer Anti-Mouse IgG Reagent (made in horse, ready-to-use)
  • 15 ml 2.5% Normal Horse Serum for blocking (ready-to-use)
  • 15 ml BLOXALL Endogenous Enzyme Blocking Solution
  • ImmPACT DAB EqV Substrate (7.5 ml Reagent 1 + 7.5 ml Reagent 2)

The post ImmPRESS® HRP Horse Anti-Mouse IgG PLUS Polymer Kit, Peroxidase appeared first on VectorLabs.

]]>

Description

The ImmPRESS polymerized reporter enzyme staining system uses novel conjugation and micropolymer chemistries to create a highly sensitive, ready-to-use, one-step detection system. This unique micropolymer of highly active enzyme (peroxidase) is attached to our affinity purified secondary antibodies, producing reagents with outstanding sensitivity and low background. The inclusion of BLOXALL® endogenous enzyme quenching solution and ImmPACT® DAB EqV substrate in the PLUS kit provides a more complete detection system.

Features:

  • Enhanced accessibility to nuclear and membrane antigens
  • Ready-to-use in a convenient dropper bottle
  • Shorter assay time
  • Simplified multiple labeling

The ImmPRESS (15 ml) Kit will stain approximately 75-150 sections.

Specifications

Unit Size1 Kit
ApplicationsImmunohistochemistry / Immunocytochemistry, In situ hybridization, ELISAs
TechnologyMicropolymer Reagents
Detection Enzyme(s)Peroxidase
ConjugateMicropolymer HRP
FormatReady-to-Use

Kit Contents:

  • 15 ml ImmPRESS (Peroxidase) Polymer Anti-Mouse IgG Reagent (made in horse, ready-to-use)
  • 15 ml 2.5% Normal Horse Serum for blocking (ready-to-use)
  • 15 ml BLOXALL Endogenous Enzyme Blocking Solution
  • ImmPACT DAB EqV Substrate (7.5 ml Reagent 1 + 7.5 ml Reagent 2)

Documents

Technical Information

The peroxidase micropolymers of the ImmPRESS HRP polymer reagent limit steric interference and provide enhanced accessibility to the target, avoiding the disadvantages of other polymer systems that use large dextrans or other macromolecules as backbones. The result is crisp, strong staining of antibody targets, especially nuclear and membrane antigens (such as Ki67, estrogen receptor, bcl-2, CD3, CD8 and CD10) and greater sensitivity than other polymer systems.

Staining Procedure

The staining procedure is simple as shown in the diagram below. Following a blocking step with the diluted normal horse serum, sections are incubated with primary antibody. After a brief wash, the appropriate ImmPRESS Reagent is added to the sections and incubated for 30 minutes. Sections are again rinsed and the slides are developed with the peroxidase substrate of choice.

ImmPRESS SL600 jpg

 

Consider Species Cross-Reactivity

When choosing the optimal detection system for your application, it is important to consider not only the species of the primary antibody but also the species of the tissue. If the species of the primary antibody and the species of the tissue are closely related (e.g. rat and mouse), the secondary antibody may bind to endogenous IgG in the tissue section leading to background. The following options minimize background staining in these instances:

  • Use a secondary antibody specifically adsorbed to remove cross-reacting antibodies of closely-related species (e.g. ImmPRESS Anti-Mouse IgG, Rat Adsorbed).
  • Use the M.O.M.® ImmPRESS Kit (Cat. No. MP-2400) for applications of mouse primary antibodies on mouse tissue.

Product review on Biocompare.com.

 

The post ImmPRESS® HRP Horse Anti-Mouse IgG PLUS Polymer Kit, Peroxidase appeared first on VectorLabs.

]]> ImmPRESS® HRP Horse Anti-Rabbit IgG PLUS Polymer Kit, Peroxidase https://staging.vectorlabs.com/products/immpress-hrp-horse-anti-rabbit-igg-plus-kit/ Tue, 02 May 2023 20:31:43 +0000 https://staging.vectorlabs.com/?post_type=product&p=14720 Description

The ImmPRESS polymerized reporter enzyme staining system uses novel conjugation and micropolymer chemistries to create a highly sensitive, ready-to-use, one-step detection system. This unique micropolymer of highly active enzyme (peroxidase or alkaline phosphatase) is attached to our affinity purified secondary antibodies, producing reagents with outstanding sensitivity and low background. The inclusion of BLOXALL® endogenous enzyme quenching solution and ImmPACT® DAB EqV substrate in the PLUS kit provides a more complete detection system.

Features:

  • Enhanced accessibility to nuclear and membrane antigens
  • Ready-to-use in a convenient dropper bottle
  • Shorter assay time
  • Simplified multiple labeling

 

The ImmPRESS (15 ml) Kit will stain approximately 75-150 sections.

Specifications

Unit Size 1 Kit
Applications Immunohistochemistry / Immunocytochemistry, In situ hybridization, ELISAs
Technology Micropolymer Reagents
Detection Enzyme(s) Peroxidase
Conjugate Micropolymer HRP
Target Species Rabbit
Format Ready-to-Use

Kit Contents:

  • 15 ml ImmPRESS (Peroxidase) Polymer Anti-Rabbit IgG Reagent (made in horse, ready-to-use)
  • 15 ml 2.5% Normal Horse Serum for blocking (ready-to-use)
  • 15 ml BLOXALL Endogenous Enzyme Blocking Solution
  • ImmPACT DAB EqV Substrate (7.5 ml Reagent 1 + 7.5 ml Reagent 2)

The post ImmPRESS® HRP Horse Anti-Rabbit IgG PLUS Polymer Kit, Peroxidase appeared first on VectorLabs.

]]>

Description

The ImmPRESS polymerized reporter enzyme staining system uses novel conjugation and micropolymer chemistries to create a highly sensitive, ready-to-use, one-step detection system. This unique micropolymer of highly active enzyme (peroxidase or alkaline phosphatase) is attached to our affinity purified secondary antibodies, producing reagents with outstanding sensitivity and low background. The inclusion of BLOXALL® endogenous enzyme quenching solution and ImmPACT® DAB EqV substrate in the PLUS kit provides a more complete detection system.

Features:

  • Enhanced accessibility to nuclear and membrane antigens
  • Ready-to-use in a convenient dropper bottle
  • Shorter assay time
  • Simplified multiple labeling

The ImmPRESS (15 ml) Kit will stain approximately 75-150 sections.

Specifications

Unit Size1 Kit
ApplicationsImmunohistochemistry / Immunocytochemistry, In situ hybridization, ELISAs
TechnologyMicropolymer Reagents
Detection Enzyme(s)Peroxidase
ConjugateMicropolymer HRP
Target SpeciesRabbit
FormatReady-to-Use

Kit Contents:

  • 15 ml ImmPRESS (Peroxidase) Polymer Anti-Rabbit IgG Reagent (made in horse, ready-to-use)
  • 15 ml 2.5% Normal Horse Serum for blocking (ready-to-use)
  • 15 ml BLOXALL Endogenous Enzyme Blocking Solution
  • ImmPACT DAB EqV Substrate (7.5 ml Reagent 1 + 7.5 ml Reagent 2)

Documents

Technical Information

The peroxidase micropolymers of the ImmPRESS HRP polymer reagent limit steric interference and provide enhanced accessibility to the target, avoiding the disadvantages of other polymer systems that use large dextrans or other macromolecules as backbones. The result is crisp, strong staining of antibody targets, especially nuclear and membrane antigens (such as Ki67, estrogen receptor, bcl-2, CD3, CD8 and CD10) and greater sensitivity than other polymer systems.

Staining Procedure

The staining procedure is simple as shown in the diagram below. Following a blocking step with the diluted normal horse serum, sections are incubated with primary antibody. After a brief wash, the appropriate ImmPRESS Reagent is added to the sections and incubated for 30 minutes. Sections are again rinsed and the slides are developed with the peroxidase substrate of choice.

ImmPRESS SL600 jpg

 

Consider Species Cross-Reactivity

When choosing the optimal detection system for your application, it is important to consider not only the species of the primary antibody but also the species of the tissue. If the species of the primary antibody and the species of the tissue are closely related (e.g. rat and mouse), the secondary antibody may bind to endogenous IgG in the tissue section leading to background. The following options minimize background staining in these instances:

  • Use a secondary antibody specifically adsorbed to remove cross-reacting antibodies of closely-related species (e.g. ImmPRESS Anti-Mouse IgG, Rat Adsorbed).
  • Use the M.O.M.® ImmPRESS Kit (Cat. No. MP-2400) for applications of mouse primary antibodies on mouse tissue.

Product review on Biocompare.com.

 

The post ImmPRESS® HRP Horse Anti-Rabbit IgG PLUS Polymer Kit, Peroxidase appeared first on VectorLabs.

]]> ImmPRESS® HRP Universal (Horse Anti-Mouse/Rabbit IgG) PLUS Polymer Kit, Peroxidase https://staging.vectorlabs.com/products/immpress-hrp-plus-horse-anti-mouse-rabbit-igg/ Tue, 02 May 2023 20:25:30 +0000 https://staging.vectorlabs.com/?post_type=product&p=14712 Description

The ImmPRESS polymerized reporter enzyme staining system uses novel conjugation and micropolymer chemistries to create a highly sensitive, ready-to-use, one-step detection system. The Inclusion of BLOXALL® endogenous enzyme quenching solution and ImmPACT® DAB EqV substrate in the PLUS kit provides added convenience with optimized, matched reagents in one product.

Features:

  • High sensitivity and low background
  • Enhanced accessibility to nuclear and membrane antigens
  • Ready-to-use in a convenient dropper bottle
  • Shorter assay time
  • Simplified multiple labeling
  • Designed for use with both rabbit and mouse primary antibodies

The ImmPRESS (15 ml) Kit will stain approximately 75-150 sections.

Note: This polymer detection kit should not be used to stain tissues from rodents or rabbits due to its reactivity with IgG present in the tissues of these species.

Specifications

Unit Size 1 Kit
Applications Immunohistochemistry / Immunocytochemistry, In situ hybridization, ELISAs
Technology Micropolymer Reagents
Detection Enzyme(s) Peroxidase
Conjugate Micropolymer HRP
Target Species Universal (Mouse/Rabbit)
Format Ready-to-Use

Kit Contents:

  • 15 ml ImmPRESS Universal Antibody (anti-mouse IgG/anti-rabbit IgG, Peroxidase) Polymer Reagent (made in horse, ready-to-use)
  • 15 ml 2.5% Normal Horse Serum for blocking (ready-to-use)
  • 15 ml BLOXALL Endogenous Enzyme Blocking Solution
  • ImmPACT DAB EqV Substrate (7.5 ml Reagent 1 + 7.5 ml Reagent 2)

The post ImmPRESS® HRP Universal (Horse Anti-Mouse/Rabbit IgG) PLUS Polymer Kit, Peroxidase appeared first on VectorLabs.

]]>

Description

 

The ImmPRESS polymerized reporter enzyme staining system uses novel conjugation and micropolymer chemistries to create a highly sensitive, ready-to-use, one-step detection system. The Inclusion of BLOXALL® endogenous enzyme quenching solution and ImmPACT® DAB EqV substrate in the PLUS kit provides added convenience with optimized, matched reagents in one product. 

Features:

  • High sensitivity and low background
  • Enhanced accessibility to nuclear and membrane antigens
  • Ready-to-use in a convenient dropper bottle
  • Shorter assay time
  • Simplified multiple labeling
  • Designed for use with both rabbit and mouse primary antibodies

 

The ImmPRESS (15 ml) Kit will stain approximately 75-150 sections.

Note: This polymer detection kit should not be used to stain tissues from rodents or rabbits due to its reactivity with IgG present in the tissues of these species.

Specifications

Unit Size1 Kit
ApplicationsImmunohistochemistry / Immunocytochemistry, In situ hybridization, ELISAs
TechnologyMicropolymer Reagents
Detection Enzyme(s)Peroxidase
ConjugateMicropolymer HRP
Target SpeciesUniversal (Mouse/Rabbit)
FormatReady-to-Use

Kit Contents:

  • 15 ml ImmPRESS Universal Antibody (anti-mouse IgG/anti-rabbit IgG, Peroxidase) Polymer Reagent (made in horse, ready-to-use)
  • 15 ml 2.5% Normal Horse Serum for blocking (ready-to-use)
  • 15 ml BLOXALL Endogenous Enzyme Blocking Solution
  • ImmPACT DAB EqV Substrate (7.5 ml Reagent 1 + 7.5 ml Reagent 2)

Documents

Technical Information

The peroxidase micropolymers of the ImmPRESS HRP polymer reagent limit steric interference and provide enhanced accessibility to the target, avoiding the disadvantages of other polymer systems that use large dextrans or other macromolecules as backbones. The result is crisp, strong staining of antibody targets, especially nuclear and membrane antigens (such as Ki67, estrogen receptor, bcl-2, CD3, CD8 and CD10) and greater sensitivity than other polymer systems.

Staining Procedure

The staining procedure is simple as shown in the diagram below. Following a blocking step with the diluted normal horse serum, sections are incubated with primary antibody. After a brief wash, the appropriate ImmPRESS Reagent is added to the sections and incubated for 30 minutes. Sections are again rinsed and the slides are developed with the peroxidase substrate of choice.

ImmPRESS SL600 jpg

 
 

Consider Species Cross-Reactivity

When choosing the optimal detection system for your application, it is important to consider not only the species of the primary antibody but also the species of the tissue. If the species of the primary antibody and the species of the tissue are closely related (e.g. rat and mouse), the secondary antibody may bind to endogenous IgG in the tissue section leading to background. The following options minimize background staining in these instances:

  • Use a secondary antibody specifically adsorbed to remove cross-reacting antibodies of closely-related species (e.g. ImmPRESS Anti-Mouse IgG, Rat Adsorbed).
  • Use the M.O.M.® ImmPRESS Kit (Cat. No. MP-2400) for applications of mouse primary antibodies on mouse tissue.

Product review on Biocompare.com.

 

The post ImmPRESS® HRP Universal (Horse Anti-Mouse/Rabbit IgG) PLUS Polymer Kit, Peroxidase appeared first on VectorLabs.

]]> ImmPRESS®-AP Horse Anti-Goat IgG Polymer Detection Kit, Alkaline Phosphatase https://staging.vectorlabs.com/products/immpress-ap-anti-goat-igg-kit/ Tue, 02 May 2023 18:46:28 +0000 https://staging.vectorlabs.com/?post_type=product&p=14480 Description

The ImmPRESS polymerized reporter enzyme staining system uses novel conjugation and micropolymer chemistries to create a highly sensitive, ready-to-use, one-step detection system. This unique micropolymer of highly active enzyme (alkaline phosphatase) is attached to our affinity purified secondary antibodies, producing reagents with outstanding sensitivity and low background.

Features:

  • Enhanced accessibility to nuclear and membrane antigens
  • Ready-to-use in a convenient dropper bottle
  • Shorter assay time
  • Simplified multiple labeling

Kit Contents:

  • ImmPRESS-AP (alkaline phosphatase) Polymer Anti-Goat IgG Reagent (ready-to-use)
  • 2.5% Normal Horse Serum for blocking (ready-to-use)

 

The ImmPRESS (15 ml) Kit will stain approximately 75-150 sections and the ImmPRESS (50 ml) Kit will stain approximately 250-500 sections.

Specifications

Unit Size 15 ml, 50 ml
Applications Immunohistochemistry / Immunocytochemistry, In situ hybridization, ELISAs
Technology Micropolymer Reagents
Detection Enzyme(s) Alkaline Phosphatase
Conjugate Micropolymer AP
Target Species Goat
Format Ready-to-Use

The post ImmPRESS®-AP Horse Anti-Goat IgG Polymer Detection Kit, Alkaline Phosphatase appeared first on VectorLabs.

]]>

Description

The ImmPRESS polymerized reporter enzyme staining system uses novel conjugation and micropolymer chemistries to create a highly sensitive, ready-to-use, one-step detection system. This unique micropolymer of highly active enzyme (alkaline phosphatase) is attached to our affinity purified secondary antibodies, producing reagents with outstanding sensitivity and low background.  

Features:

  • Enhanced accessibility to nuclear and membrane antigens
  • Ready-to-use in a convenient dropper bottle
  • Shorter assay time
  • Simplified multiple labeling

 

Kit Contents:

  • ImmPRESS-AP (alkaline phosphatase) Polymer Anti-Goat IgG Reagent (ready-to-use)
  • 2.5% Normal Horse Serum for blocking (ready-to-use)

The ImmPRESS (15 ml) Kit will stain approximately 75-150 sections and the ImmPRESS (50 ml) Kit will stain approximately 250-500 sections.

Specifications

Unit Size15 ml, 50 ml
ApplicationsImmunohistochemistry / Immunocytochemistry, In situ hybridization, ELISAs
TechnologyMicropolymer Reagents
Detection Enzyme(s)Alkaline Phosphatase
ConjugateMicropolymer AP
Target SpeciesGoat
FormatReady-to-Use

Documents

Product FAQs

The ImmPRESS Excel kits are a two-step peroxidase polymer based detection system that include an unconjugated amplifier antibody. This intermediate amplifier antibody increases sensitivity of the assay at least three- to four-fold over that of a one-step polymer based system, by facilitating the introduction of more peroxidase enzyme at the site of specific antigen localization. This increase in sensitivity would be advantageous in instances of weak antigen expression, and to further dilute out an expensive primary antibody. The ImmPRESS Excel Amplifier kits are presented as a complete kit format that include enzyme quench, blocking serum and ImmPACT® DAB EqV peroxidase substrate, in addition to the amplifier antibody and defined ImmPRESS polymer secondary antibody. However, as the two published references below indicate, this format is still modular and allows for the substitution of different detection reagents. These references describe using ImmPACT NovaRED™ peroxidase substrate in combination with the ImmPRESS Excel Amplifier kits. Oncogenesis (2017) 6, e293; doi:10.1038/oncsis.2016.82 Tan, E.M.S., et al (2017) Front. Med. 4:162 (doi: 10.3389/fmed.2017.00162)

Yes, we conducted studies on the compatibility of the ImmPRESS HRP polymer reagents with three commercially available autostainers (Agilent/Dako Autostainer Plus, Leica Bond Rx, and Ventana Discovery Ultra). We showed that our reagents are suitable for IHC detection on each of these platforms. The ImmPRESS polymer reagents and enzyme substrates generated equivalent IHC staining results compared to reagents from the instrument manufacturer. Some modifications in the protocol were performed to optimize the signal-to-noise ratio, such as a shorter incubation time for the ImmPRESS polymer and increase in the number of buffer washes following polymer incubation. Please see the following application note for more details about each automated platform:https://staging.vectorlabs.com/wp-content/uploads/2022/11/VL_LIT3023_ImmPRESS_AppNote_4.pdf

Technical Information

The ImmPRESS-AP Alkaline Phosphatase Polymer Reagent micropolymer technology limits steric interference and provides enhanced accessibility to the target avoiding the disadvantages of other polymer systems that use large dextrans or other macromolecules as backbones.  The result is crisp, strong staining of antibody targets, especially nuclear and membrane antigens, and  greater sensitivity than conventional alkaline phosphatase antibody conjugates and other alkaline phosphatase polymer systems for immunohistochemistry and other applications. 

Staining Procedure

The staining procedure is simple as shown in the diagram below. Following a blocking step with the diluted normal horse serum, sections are incubated with primary antibody. After a brief wash, the appropriate ImmPRESS Reagent is added to the sections and incubated for 30 minutes. Sections are again rinsed and the slides are developed with the peroxidase substrate of choice.

ImmPRESS SL600 jpg

 

Consider Species Cross-Reactivity

When choosing the optimal detection system for your application, it is important to consider not only the species of the primary antibody but also the species of the tissue. If the species of the primary antibody and the species of the tissue are closely related (e.g. rat and mouse), the secondary antibody may bind to endogenous IgG in the tissue section leading to background. The following options minimize background staining in these instances:

  • Use a secondary antibody specifically adsorbed to remove cross-reacting antibodies of closely-related species (e.g. ImmPRESS Anti-Mouse IgG, Rat Adsorbed).
  • Use the M.O.M.® ImmPRESS Kit (Cat. No. MP-2400) for applications of mouse primary antibodies on mouse tissue.

The post ImmPRESS®-AP Horse Anti-Goat IgG Polymer Detection Kit, Alkaline Phosphatase appeared first on VectorLabs.

]]> Horse Anti-Mouse/Rabbit IgG Antibody (H+L) (Universal), Biotinylated, R.T.U. https://staging.vectorlabs.com/products/biotinylated-horse-anti-mouse-rabbit-igg/ Tue, 02 May 2023 16:55:36 +0000 https://staging.vectorlabs.com/?post_type=product&p=14432 Description

This R.T.U biotinylated universal secondary antibody binds equally well to both mouse IgG (H+L) and rabbit IgG (H+L) primary antibodies. This antibody has significant cross-reactivity for rodent IgG and is not recommended for use in staining rodent tissue.

Features:

  • Affinity-purified, ultrapure, high affinity antibody
  • Thoroughly adsorbed against serum and immunoglobulins from potentially interfering species
  • Recognizes both heavy and light chains (H+L)
  • Biotinylated to ensure the maximum degree of labeling without compromising the specificity or affinity of the antibody
  • Can be used for tissue and cell staining, ELISAs, and blots
  • Included in the VECTASTAIN® ABC kits.
  • Supplied in a convenient ready-to-use prediluted, stabilized solution
  • This pan-specific, biotinylated “universal” secondary antibody is specially formulated to recognize IgG (H+L) primary antibodies from mouse and rabbit

 

Note: This antibody has significant cross-reactivity for rodent IgG and is not recommended for use in staining rodent tissue.
Note: This product is the same biotinylated secondary antibody included in the VECTASTAIN Elite® ABC Kit (Universal; PK-6200) and VECTASTAIN ABC-AP Kit (Universal; AK-5200), but is not the same as that provided in the VECTASTAIN Universal Quick Kit, PK-8800.

Specifications

Unit Size 50 ml
Applications Immunohistochemistry / Immunocytochemistry, Immunofluorescence, In situ hybridization, Blotting Applications, Elispot, ELISAs
Concentration Ready-to-use solution of biotinylated horse anti-mouse/anti-rabbit IgG in PBS* containing carrier protein and 0.08% sodium azide as preservative.
Recommended Storage 2-8 °C
Recommended Usage Following incubation with primary antibody, wash specimens in buffer.Apply RTU Biotinylated Anti-Mouse/Anti-Rabbit IgG and incubate for 30 minutes at room temperature.Wash in buffer for 5 minutes and follow with avidin or streptavidin based detection reagent such as RTU VECTASTAIN®® ABC Reagent(PK-7100). * 10mM phosphate, pH 7.8, 0.15M NaCl.
Target Species Universal (Mouse/Rabbit)
Conjugate Biotinylated
Host Species Horse
Format Ready-to-Use

The post Horse Anti-Mouse/Rabbit IgG Antibody (H+L) (Universal), Biotinylated, R.T.U. appeared first on VectorLabs.

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Description

This R.T.U biotinylated universal secondary antibody binds equally well to both mouse IgG (H+L) and rabbit IgG (H+L) primary antibodies. This antibody has significant cross-reactivity for rodent IgG and is not recommended for use in staining rodent tissue.

Features:

  • Affinity-purified, ultrapure, high affinity antibody 
  • Thoroughly adsorbed against serum and immunoglobulins from potentially interfering species 
  • Recognizes both heavy and light chains (H+L) 
  • Biotinylated to ensure the maximum degree of labeling without compromising the specificity or affinity of the antibody 
  • Can be used for tissue and cell staining, ELISAs, and blots 
  • Included in the VECTASTAIN® ABC kits. 
  • Supplied in a convenient ready-to-use prediluted, stabilized solution
  • This pan-specific, biotinylated “universal” secondary antibody is specially formulated to recognize IgG (H+L) primary antibodies from mouse and rabbit

Note: This antibody has significant cross-reactivity for rodent IgG and is not recommended for use in staining rodent tissue.
Note: This product is the same biotinylated secondary antibody included in the VECTASTAIN Elite® ABC Kit (Universal; PK-6200) and VECTASTAIN ABC-AP Kit (Universal; AK-5200), but is not the same as that provided in the VECTASTAIN Universal Quick Kit, PK-8800.

Specifications

Unit Size50 ml
ApplicationsImmunohistochemistry / Immunocytochemistry, Immunofluorescence, In situ hybridization, Blotting Applications, Elispot, ELISAs
ConcentrationReady-to-use solution of biotinylated horse anti-mouse/anti-rabbit IgG in PBS* containing carrier protein and 0.08% sodium azide as preservative.
Recommended Storage2-8 °C
Recommended UsageFollowing incubation with primary antibody, wash specimens in buffer.Apply RTU Biotinylated Anti-Mouse/Anti-Rabbit IgG and incubate for 30 minutes at room temperature.Wash in buffer for 5 minutes and follow with avidin or streptavidin based detection reagent such as RTU VECTASTAIN®® ABC Reagent(PK-7100). * 10mM phosphate, pH 7.8, 0.15M NaCl.
Target SpeciesUniversal (Mouse/Rabbit)
ConjugateBiotinylated
Host SpeciesHorse
FormatReady-to-Use

Documents

Citations

Technical Information

Vector Laboratories affinity-purified antibodies are of unmatched quality. These antibodies are prepared using proprietary immunization schedules that produce high affinity antibodies. The antibodies are then purified by affinity chromatography, and cross-reactivities that are likely to interfere with specific labeling are removed by solid phase adsorption techniques. The biotinylated secondary antibodies are conjugated to ensure the maximum degree of labeling without compromising the specificity or affinity of the antibody. These antibodies are subjected to rigorous quality control assays and can be used for tissue and cell staining, ELISAs, and blots.

This biotinylated Universal secondary antibody is produced in horse and binds equally well to both mouse IgG (H+L) and rabbit IgG (H+L) primary antibodies. The high staining quality and sensitivity achieved using this product is equivalent to that obtained with either our biotinylated anti-mouse IgG (H+L) or biotinylated anti-rabbit IgG (H+L) used individually. The convenience of using a single biotinylated secondary antibody for both species of primary and the elimination of possible mistakes in using the wrong secondary antibody is an advantage of this product.

 
Ready-to-Use (R.T.U.) Biotinylated Secondary Antibodies are stabilized, prediluted, ready-to-use solutions of our biotinylated secondary antibodies. These antibody solutions are provided in convenient dropper bottle format for ease of use.

The post Horse Anti-Mouse/Rabbit IgG Antibody (H+L) (Universal), Biotinylated, R.T.U. appeared first on VectorLabs.

]]> Horse Anti-Goat IgG Antibody (H+L), Biotinylated, R.T.U. https://staging.vectorlabs.com/products/rtu-biotinylated-horse-anti-goat-igg/ Tue, 02 May 2023 16:50:00 +0000 https://staging.vectorlabs.com/?post_type=product&p=14414 Description

The Ready-to-Use (R.T.U.) Biotinylated Horse Anti-Goat IgG Antibody is used in an avidin-biotin or streptavidin-biotin detection system. These antibodies can be used for tissue and cell staining, ELISAs, and blots.

Features:

  • Suitable for use with goat, sheep, and bovine IgG primary antibodies
  • Recognizes both heavy and light chains (H+L)

Specifications

More Information
Unit Size 50 ml
Applications Immunohistochemistry / Immunocytochemistry, Immunofluorescence, In situ hybridization, Blotting Applications, Elispot, ELISAs
Concentration Ready-to-use solution of biotinylated horse anti-goat IgG in PBS* containing carrier protein and 0.08% sodium azide as preservative.
Recommended Storage 2-8 °C
Recommended Usage Following incubation with primary antibody, wash specimens in buffer.Apply RTU Biotinylated Anti-Goat IgG and incubate for 30 minutes at room temperature.Wash inbuffer for 5 minutes and follow with avidin- or streptavidin-based detection reagent such as RTU VECTASTAIN® Elite® ABC Reagent (PK-7100). * 10mM sodium phosphate, pH 7.8, 0.15M NaCl.
Target Species Goat
Conjugate Biotinylated
Host Species Horse
Format Ready-to-Use

The post Horse Anti-Goat IgG Antibody (H+L), Biotinylated, R.T.U. appeared first on VectorLabs.

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Description

The Ready-to-Use (R.T.U.) Biotinylated Horse Anti-Goat IgG Antibody is used in an avidin-biotin or streptavidin-biotin detection system. These antibodies can be used for tissue and cell staining, ELISAs, and blots.

Features:

  • Suitable for use with goat, sheep, and bovine IgG primary antibodies
  • Recognizes both heavy and light chains (H+L) 

Specifications

More Information
Unit Size50 ml
ApplicationsImmunohistochemistry / Immunocytochemistry, Immunofluorescence, In situ hybridization, Blotting Applications, Elispot, ELISAs
ConcentrationReady-to-use solution of biotinylated horse anti-goat IgG in PBS* containing carrier protein and 0.08% sodium azide as preservative.
Recommended Storage2-8 °C
Recommended UsageFollowing incubation with primary antibody, wash specimens in buffer.Apply RTU Biotinylated Anti-Goat IgG and incubate for 30 minutes at room temperature.Wash inbuffer for 5 minutes and follow with avidin- or streptavidin-based detection reagent such as RTU VECTASTAIN® Elite® ABC Reagent (PK-7100). * 10mM sodium phosphate, pH 7.8, 0.15M NaCl.
Target SpeciesGoat
ConjugateBiotinylated
Host SpeciesHorse
FormatReady-to-Use

Documents

Technical Information

Vector Laboratories affinity-purified antibodies are of unmatched quality. These antibodies are prepared using proprietary immunization schedules that produce high affinity antibodies. The antibodies are then purified by affinity chromatography, and cross-reactivities that are likely to interfere with specific labeling are removed by solid phase adsorption techniques. The biotinylated secondary antibodies are conjugated to ensure the maximum degree of labeling without compromising the specificity or affinity of the antibody. These antibodies are subjected to rigorous quality control assays and can be used for tissue and cell staining, ELISAs, and blots.

Ready-to-Use (R.T.U.) Biotinylated Secondary Antibodies are stabilized, prediluted, ready-to-use solutions of our biotinylated secondary antibodies. These antibody solutions are provided in convenient dropper bottle format for ease of use.

The use of bovine products such as casein, serum, albumin or non-fat dry milk as blocking agents in ELISA or blot assays may produce a high background due to cross-reactivity of this antibody with bovine immunoglobulins that may be present. If this antibody is to be used in these applications, blocking with Animal-Free Blocker® solution (Cat. No. SP-5030) is recommended.

The post Horse Anti-Goat IgG Antibody (H+L), Biotinylated, R.T.U. appeared first on VectorLabs.

]]> Horse Anti-Mouse IgG Antibody (H+L), Biotinylated, R.T.U. https://staging.vectorlabs.com/products/rtu-biotinylated-horse-anti-mouse-igg/ Tue, 02 May 2023 16:44:38 +0000 https://staging.vectorlabs.com/?post_type=product&p=14403 Description

R.T.U. Biotinylated Horse Anti-Mouse IgG Antibody is used in an avidin-biotin or streptavidin-biotin detection system. These antibodies can be used for tissue and cell staining, ELISAs, and blots.

Features:

  • Affinity-purified, ultrapure, high affinity antibody
  • Thoroughly adsorbed against serum and immunoglobulins from potentially interfering species. (For rat tissue applications, please see anti-mouse, rat adsorbed, secondary antibodies.)
  • Recognizes both heavy and light chains (H+L)
  • Biotinylated to ensure the maximum degree of labeling without compromising the specificity or affinity of the antibody
  • Can be used for tissue and cell staining, ELISAs, and blots
  • Included in the VECTASTAIN® ABC kits
  • Supplied in a convenient ready-to-use prediluted, stabilized solution

Specifications

Unit Size 50 ml
Applications Immunohistochemistry / Immunocytochemistry, Immunofluorescence, In situ hybridization, Blotting Applications, Elispot, ELISAs
Concentration Ready-to-use solution of biotinylated horse anti-mouse IgG in PBS* containing carrier protein and 0.08% sodium azide as preservative.
Recommended Storage 2-8 °C
Recommended Usage Following incubation with primary antibody, wash specimens in buffer.Apply RTU Biotinylated Anti-Mouse IgG and incubate for 30 minutes at room temperature.Wash in buffer for 5 minutes and follow with avidin- or streptavidin-based detection reagent such as RTU VECTASTAIN® ABC Reagent (PK-7100). * 10mM sodium phosphate, pH 7.8, 0.15M NaCl.
Target Species Mouse
Conjugate Biotinylated
Host Species Horse
Format Ready-to-Use

The post Horse Anti-Mouse IgG Antibody (H+L), Biotinylated, R.T.U. appeared first on VectorLabs.

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Description

R.T.U. Biotinylated Horse Anti-Mouse IgG Antibody is used in an avidin-biotin or streptavidin-biotin detection system. These antibodies can be used for tissue and cell staining, ELISAs, and blots.

Features:

  • Affinity-purified, ultrapure, high affinity antibody 
  • Thoroughly adsorbed against serum and immunoglobulins from potentially interfering species. (For rat tissue applications, please see anti-mouse, rat adsorbed, secondary antibodies.) 
  • Recognizes both heavy and light chains (H+L) 
  • Biotinylated to ensure the maximum degree of labeling without compromising the specificity or affinity of the antibody 
  • Can be used for tissue and cell staining, ELISAs, and blots 
  • Included in the VECTASTAIN® ABC kits
  • Supplied in a convenient ready-to-use prediluted, stabilized solution 

Specifications

Unit Size50 ml
ApplicationsImmunohistochemistry / Immunocytochemistry, Immunofluorescence, In situ hybridization, Blotting Applications, Elispot, ELISAs
ConcentrationReady-to-use solution of biotinylated horse anti-mouse IgG in PBS* containing carrier protein and 0.08% sodium azide as preservative.
Recommended Storage2-8 °C
Recommended UsageFollowing incubation with primary antibody, wash specimens in buffer.Apply RTU Biotinylated Anti-Mouse IgG and incubate for 30 minutes at room temperature.Wash in buffer for 5 minutes and follow with avidin- or streptavidin-based detection reagent such as RTU VECTASTAIN® ABC Reagent (PK-7100). * 10mM sodium phosphate, pH 7.8, 0.15M NaCl.
Target SpeciesMouse
ConjugateBiotinylated
Host SpeciesHorse
FormatReady-to-Use

Documents

Product FAQs

Make a fresh working solution of DAB substrate per instructions. Place a small volume (~1 mL) of this DAB substrate into a clean glass test tube. To this 1 ml aliquot, add one drop (~50 µL) of Reagent B only from the VECTASTAIN® Elite ABC kit. If the HRP enzyme is active and the DAB reacts with it, an immediate color change will be observed. This indicates the end detection reagents are working appropriately.

Citations

Technical Information

Vector Laboratories affinity-purified antibodies are of unmatched quality. These antibodies are prepared using proprietary immunization schedules that produce high affinity antibodies. The antibodies are then purified by affinity chromatography, and cross-reactivities that are likely to interfere with specific labeling are removed by solid phase adsorption techniques. The biotinylated secondary antibodies are conjugated to ensure the maximum degree of labeling without compromising the specificity or affinity of the antibody. These antibodies are subjected to rigorous quality control assays.

The post Horse Anti-Mouse IgG Antibody (H+L), Biotinylated, R.T.U. appeared first on VectorLabs.

]]> Horse Anti-Rabbit IgG Antibody (H+L), Biotinylated, R.T.U. https://staging.vectorlabs.com/products/rtu-biotinylated-horse-anti-rabbit-igg/ Tue, 02 May 2023 16:41:39 +0000 https://staging.vectorlabs.com/?post_type=product&p=14396 Description

The R.T.U. biotinylated horse anti-rabbit IgG secondary antibody is used in an avidin-biotin or streptavidin-biotin detection system. These antibodies can be used for tissue and cell staining, ELISAs, and blots.

Features:

  • Recognizes both heavy and light chains (H+L)
  • Biotinylated to ensure the maximum degree of labeling without compromising the specificity or affinity of the antibody
  • Supplied in a convenient ready-to-use prediluted, stabilized solution

Specifications

Unit Size 50 ml
Applications Immunohistochemistry / Immunocytochemistry, Immunofluorescence, In situ hybridization, Blotting Applications, Elispot, ELISAs
Concentration Ready-to-use solution of biotinylated horse anti-rabbit IgG in PBS* containing carrier protein and 0.08% sodium azide as preservative.
Recommended Storage 2-8 °C
Recommended Usage Following incubation with primary antibody, wash specimens in buffer.Apply RTU Biotinylated Anti-Rabbit IgG and incubate for 30 minutes at room temperature.Wash in buffer for 5 minutes and follow with avidin- or streptavidin-based detection reagent such as RTU VECTASTAIN® ABC Reagent (PK-7100).
Target Species Rabbit
Conjugate Biotinylated
Host Species Horse
Format Ready-to-Use

The post Horse Anti-Rabbit IgG Antibody (H+L), Biotinylated, R.T.U. appeared first on VectorLabs.

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Description

The R.T.U. biotinylated horse anti-rabbit IgG secondary antibody is used in an avidin-biotin or streptavidin-biotin detection system. These antibodies can be used for tissue and cell staining, ELISAs, and blots. 

Features:

  • Recognizes both heavy and light chains (H+L) 
  • Biotinylated to ensure the maximum degree of labeling without compromising the specificity or affinity of the antibody 
  • Supplied in a convenient ready-to-use prediluted, stabilized solution 

Specifications

Unit Size50 ml
ApplicationsImmunohistochemistry / Immunocytochemistry, Immunofluorescence, In situ hybridization, Blotting Applications, Elispot, ELISAs
ConcentrationReady-to-use solution of biotinylated horse anti-rabbit IgG in PBS* containing carrier protein and 0.08% sodium azide as preservative.
Recommended Storage2-8 °C
Recommended UsageFollowing incubation with primary antibody, wash specimens in buffer.Apply RTU Biotinylated Anti-Rabbit IgG and incubate for 30 minutes at room temperature.Wash in buffer for 5 minutes and follow with avidin- or streptavidin-based detection reagent such as RTU VECTASTAIN® ABC Reagent (PK-7100).
Target SpeciesRabbit
ConjugateBiotinylated
Host SpeciesHorse
FormatReady-to-Use

Documents

Citations

Technical Information

Vector Laboratories affinity-purified antibodies are of unmatched quality. These antibodies are prepared using proprietary immunization schedules that produce high affinity antibodies. The antibodies are then purified by affinity chromatography, and cross-reactivities that are likely to interfere with specific labeling are removed by solid phase adsorption techniques. The biotinylated secondary antibodies are conjugated to ensure the maximum degree of labeling without compromising the specificity or affinity of the antibody. 

These antibody solutions are provided in convenient dropper bottle format for ease of use.

Biotinylated Horse Anti-Rabbit IgG (H+L) Antibody can be used in single antigen labeling or paired with our biotinylated horse anti-mouse IgG in multiple antigen labeling applications.

This antibody can be detected with the VECTASTAIN® ABC systems or other (strept)avidin reagent

The post Horse Anti-Rabbit IgG Antibody (H+L), Biotinylated, R.T.U. appeared first on VectorLabs.

]]> ImmPRESS®-VR Horse Anti-Mouse IgG Polymer Detection Kit, Peroxidase, Veterinary Reagent https://staging.vectorlabs.com/products/immpress-hrp-vr-horse-anti-mouse-igg-kit/ Tue, 02 May 2023 16:20:15 +0000 https://staging.vectorlabs.com/?post_type=product&p=14355 Description

The ImmPRESS Polymerized Reporter Enzyme Staining System is an enzymatic, one-step detection kit. ImmPRESS Polymer Reagents consist of unique micropolymers of highly active peroxidase enzyme attached to highly cross-adsorbed, affinity purified secondary antibodies. The ImmPRESS Polymer Reagents produce outstanding immunohistochemistry and immunocytochemistry results due to increased target accessibility, binding specificity, and signal intensity along with low background staining.

ImmPRESS VR Polymer Detection Reagents are additionally cross-adsorbed to ensure minimal cross-reactivity against endogenous tissue elements in animal species commonly used for diagnostics and research-based animal model systems.

Features:

  • Enhanced accessibility to nuclear and membrane antigens
  • Ready-to-use in a convenient dropper bottle
  • Shorter assay time
  • Simplified multiple labeling
  • Minimal cross-reactivity with bovine, goat, sheep, pig, horse, cat, dog, and rabbit (as well as human). For mouse and rat tissues, separate kits are recommended (for rat, MP-7422; for mouse, MP-2400).

Specifications

 
Unit Size 15 ml
Applications Immunohistochemistry / Immunocytochemistry, In situ hybridization, ELISAs
Technology Micropolymer Reagents
Detection Enzyme(s) Peroxidase
Conjugate Micropolymer HRP
Target Species Mouse
Format Ready-to-Use

The post ImmPRESS®-VR Horse Anti-Mouse IgG Polymer Detection Kit, Peroxidase, Veterinary Reagent appeared first on VectorLabs.

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Description

The ImmPRESS Polymerized Reporter Enzyme Staining System is an enzymatic, one-step detection kit. ImmPRESS Polymer Reagents consist of unique micropolymers of highly active peroxidase enzyme attached to highly cross-adsorbed, affinity purified secondary antibodies. The ImmPRESS Polymer Reagents produce outstanding immunohistochemistry and immunocytochemistry results due to increased target accessibility, binding specificity, and signal intensity along with low background staining.

ImmPRESS VR Polymer Detection Reagents are additionally cross-adsorbed to ensure minimal cross-reactivity against endogenous tissue elements in animal species commonly used for diagnostics and research-based animal model systems.

Features:

  • Enhanced accessibility to nuclear and membrane antigens
  • Ready-to-use in a convenient dropper bottle
  • Shorter assay time
  • Simplified multiple labeling
  • Minimal cross-reactivity with bovine, goat, sheep, pig, horse, cat, dog, and rabbit (as well as human). For mouse and rat tissues, separate kits are recommended (for rat, MP-7422; for mouse, MP-2400).

Specifications

 
Unit Size15 ml
ApplicationsImmunohistochemistry / Immunocytochemistry, In situ hybridization, ELISAs
TechnologyMicropolymer Reagents
Detection Enzyme(s)Peroxidase
ConjugateMicropolymer HRP
Target SpeciesMouse
FormatReady-to-Use

Kit Contents:

  • ImmPRESS VR Polymer Anti-Mouse IgG Reagent (made in horse, ready-to-use)
  • 2.5% Normal Horse Serum for blocking (ready-to-use)
  • The ImmPRESS (15 ml) Kit will stain approximately 75-150 sections.

Documents

Product FAQs

The ImmPRESS Excel kits are a two-step peroxidase polymer based detection system that include an unconjugated amplifier antibody. This intermediate amplifier antibody increases sensitivity of the assay at least three- to four-fold over that of a one-step polymer based system, by facilitating the introduction of more peroxidase enzyme at the site of specific antigen localization. This increase in sensitivity would be advantageous in instances of weak antigen expression, and to further dilute out an expensive primary antibody. The ImmPRESS Excel Amplifier kits are presented as a complete kit format that include enzyme quench, blocking serum and ImmPACT® DAB EqV peroxidase substrate, in addition to the amplifier antibody and defined ImmPRESS polymer secondary antibody. However, as the two published references below indicate, this format is still modular and allows for the substitution of different detection reagents. These references describe using ImmPACT NovaRED™ peroxidase substrate in combination with the ImmPRESS Excel Amplifier kits. Oncogenesis (2017) 6, e293; doi:10.1038/oncsis.2016.82 Tan, E.M.S., et al (2017) Front. Med. 4:162 (doi: 10.3389/fmed.2017.00162)

Yes, we conducted studies on the compatibility of the ImmPRESS HRP polymer reagents with three commercially available autostainers (Agilent/Dako Autostainer Plus, Leica Bond Rx, and Ventana Discovery Ultra). We showed that our reagents are suitable for IHC detection on each of these platforms. The ImmPRESS polymer reagents and enzyme substrates generated equivalent IHC staining results compared to reagents from the instrument manufacturer. Some modifications in the protocol were performed to optimize the signal-to-noise ratio, such as a shorter incubation time for the ImmPRESS polymer and increase in the number of buffer washes following polymer incubation. Please see the following application note for more details about each automated platform:https://staging.vectorlabs.com/wp-content/uploads/2022/11/VL_LIT3023_ImmPRESS_AppNote_4.pdf

Citations

Technical Information

For mouse and rat tissues, separate kits are recommended (for rat, MP-7422; for mouse, MP-2400).

Staining Procedure

The staining procedure is simple as shown in the diagram below. Following a blocking step with the diluted normal horse serum, sections are incubated with primary antibody. After a brief wash, the appropriate ImmPRESS Reagent is added to the sections and incubated for 30 minutes. Sections are again rinsed and the slides are developed with the peroxidase substrate of choice.

ImmPRESS SL600 jpg

 
Product review on Biocompare.com.
 

The post ImmPRESS®-VR Horse Anti-Mouse IgG Polymer Detection Kit, Peroxidase, Veterinary Reagent appeared first on VectorLabs.

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