Histology – VectorLabs https://staging.vectorlabs.com From linker design and synthesis, bioconjugated design and manufacturing, biomolecule labeling and functionalization, and detection system and solutions, our team is here to help you move forward, with impact. Wed, 22 Oct 2025 17:14:30 +0000 en-US hourly 1 https://wordpress.org/?v=6.9 https://staging.vectorlabs.com/wp-content/uploads/2024/11/V-favicon-02-100x100.png Histology – VectorLabs https://staging.vectorlabs.com 32 32 Alcian Blue (pH 2.5) Stain Kit https://staging.vectorlabs.com/products/alcian-blue-ph-2-5-stain-kit/ Tue, 02 May 2023 19:19:38 +0000 https://staging.vectorlabs.com/?post_type=product&p=14506 Description

The Alcian Blue (pH 2.5) Stain Kit is intended for use in the histological visualization of sulfated and carboxylated acid mucopolysaccharides and sulfated and carboxylated sialomucins (glycoproteins).

  • Acidic Sulfated Mucosubstances: Blue
  • Hyaluronic Acid: Blue
  • Sialomucins: Blue
  • Nuclei: Red

 

Control Tissue: Small Intestine, Appendix, Colon

Specifications

Unit Size 1 Kit
Applications Histology
Microscopy Brightfield
Cellular Stain Nucleus, Glycoproteins
Color Blue, Red
Format Ready-to-Use

Components:

  • Alcian Blue Solution (pH 2.5), 30 ml
  • Nuclear Fast Red (Enhanced), 30 ml
  • Acetic Acid Solution (3%), 60 ml (30 ml x 2)

The post Alcian Blue (pH 2.5) Stain Kit appeared first on VectorLabs.

]]>

Description

The Alcian Blue (pH 2.5) Stain Kit is intended for use in the histological visualization of sulfated and carboxylated acid mucopolysaccharides and sulfated and carboxylated sialomucins (glycoproteins).

  • Acidic Sulfated Mucosubstances: Blue
  • Hyaluronic Acid: Blue
  • Sialomucins: Blue
  • Nuclei: Red

Control Tissue: Small Intestine, Appendix, Colon

Specifications

Unit Size1 Kit
ApplicationsHistology
MicroscopyBrightfield
Cellular StainNucleus, Glycoproteins
ColorBlue, Red
FormatReady-to-Use

Components:

  • Alcian Blue Solution (pH 2.5), 30 ml
  • Nuclear Fast Red (Enhanced), 30 ml
  • Acetic Acid Solution (3%), 60 ml (30 ml x 2)

Documents

Citations

Technical Information

Components:

  • Alcian Blue Solution (pH 2.5), 30 ml
  • Nuclear Fast Red (Enhanced), 30 ml
  • Acetic Acid Solution (3%),  60 ml (30 ml x 2)

Notes:

a) Intensity should be optimized for each tissue type, fixative used, and presentation (frozen or paraffin-embedded) 

b) Use standard laboratory precautions to avoid contact with skin and eyes. 

c) Harmful if swallowed. Do not take internally.

d) These reagents are for research use only.

e) Follow all federal, state and local regulations for disposal. 

The post Alcian Blue (pH 2.5) Stain Kit appeared first on VectorLabs.

]]> Hematoxylin and Eosin Stain Kit https://staging.vectorlabs.com/products/hematoxylin-and-eosin-stain-kit/ Tue, 02 May 2023 18:56:47 +0000 https://staging.vectorlabs.com/?post_type=product&p=14498 Description

The Hematoxylin and Eosin Stain Kit is intended for use in histology and cytology applications. Included in this kit is a modified Eosin that provides the benefits of a traditional alcoholic formulation with significant improvements in usability. Advantages include lower evaporation rate, better color patterns, and improved surface tension to remain on tissue section. Our Hematoxylin produces crisp, intense blue nuclei providing optimal contrast to the Eosin stained cytoplasm.

Specifications

 
Unit Size 1 Kit
Applications Histology
Microscopy Brightfield
Cellular Stain Nucleus, Cytoplasm
Color Blue, Pink
Format Ready-to-Use

The post Hematoxylin and Eosin Stain Kit appeared first on VectorLabs.

]]>

Description

The Hematoxylin and Eosin Stain Kit is intended for use in histology and cytology applications. Included in this kit is a modified Eosin that provides the benefits of a traditional alcoholic formulation with significant improvements in usability. Advantages include lower evaporation rate, better color patterns, and improved surface tension to remain on tissue section. Our Hematoxylin produces crisp, intense blue nuclei providing optimal contrast to the Eosin stained cytoplasm.

Specifications

 
Unit Size1 Kit
ApplicationsHistology
MicroscopyBrightfield
Cellular StainNucleus, Cytoplasm
ColorBlue, Pink
FormatReady-to-Use

Documents

Citations

Technical Information

Colors:

  • Cytoplasm:  Light Pink
  • Erythrocytes:  Pink/Red
  • Collagen:  Pink
  • Nuclei:  Blue
  • Muscle:  Pink/Rose

Control Tissues:  Small intestine, Appendix, Colon, Prostate

Components:

  • Hematoxylin, Mayer’s (Modifed), 30 ml
  • Bluing Reagent, 30 ml
  • Eosin Y Solution (Modified), 30 ml

Notes:

a) Intensity should be optimized for each tissue type, fixative used, and presentation (frozen or paraffin-embedded)

b) Use standard laboratory precautions to avoid contact with skin and eyes.

c) Harmful if swallowed. Do not take internally.

d) These reagents are for research use only.

e) Follow all federal, state and local regulations for disposal.

The post Hematoxylin and Eosin Stain Kit appeared first on VectorLabs.

]]> Hematoxylin QS Counterstain https://staging.vectorlabs.com/products/vector-hematoxylin-qs-counterstain/ Wed, 26 Apr 2023 14:53:50 +0000 https://staging.vectorlabs.com/?post_type=product&p=12257 Description

Vector Hematoxylin QS (H-3404), a modification of Mayer’s hematoxylin developed especially for immunocytochemistry, provides crisp blue nuclear staining. This formula is ready-to-use without filtration, requires no separate bluing step, and stains in less than 45 seconds.

Features:

  • Provides excellent color contrast with most commonly used peroxidase and alkaline phosphatase substrates
  • Suitable for use with nonaqueous and aqueous mountants
  • Mercury-free

Specifications

Unit Size 100 ml
Applications Immunohistochemistry / Immunocytochemistry, In situ hybridization
Format Ready-to-Use

The post Hematoxylin QS Counterstain appeared first on VectorLabs.

]]>

Description

Vector Hematoxylin QS (H-3404), a modification of Mayer’s hematoxylin developed especially for immunocytochemistry, provides crisp blue nuclear staining. This formula is ready-to-use without filtration, requires no separate bluing step, and stains in less than 45 seconds.

Features:

  • Provides excellent color contrast with most commonly used peroxidase and alkaline phosphatase substrates
  • Suitable for use with nonaqueous and aqueous mountants
  • Mercury-free

Specifications

Unit Size100 ml
ApplicationsImmunohistochemistry / Immunocytochemistry, In situ hybridization
FormatReady-to-Use

Documents

Citations

Technical Information

Counterstain/Substrate Compatibility Table

This table is designed as a reference to determine the optimal counterstain/substrate combination for your application.

cstaincomp jpg

Optimize Counterstain Intensity

The post Hematoxylin QS Counterstain appeared first on VectorLabs.

]]> Nuclear Fast Red Counterstain https://staging.vectorlabs.com/products/vector-nuclear-fast-red-counterstain/ Wed, 26 Apr 2023 04:51:16 +0000 https://staging.vectorlabs.com/?post_type=product&p=12244 Description

Nuclei stained with Vector® Nuclear Fast Red (H-3403) have a pink appearance. Vector Nuclear Fast Red is an excellent choice in multiple labeling applications when hematoxylin obscures the substrate colors.

Features:

  • Good contrast with a variety of substrates
  • Rapid staining procedure requiring only one step
  • Suitable for use with non-aqueous mountants

Specifications

Unit Size 500 ml
Applications Immunohistochemistry / Immunocytochemistry, In situ hybridization
Format Ready-to-Use

The post Nuclear Fast Red Counterstain appeared first on VectorLabs.

]]>

Description

Nuclei stained with Vector® Nuclear Fast Red (H-3403) have a pink appearance. Vector Nuclear Fast Red is an excellent choice in multiple labeling applications when hematoxylin obscures the substrate colors.

Features:

  • Good contrast with a variety of substrates
  • Rapid staining procedure requiring only one step
  • Suitable for use with non-aqueous mountants

Specifications

Unit Size500 ml
ApplicationsImmunohistochemistry / Immunocytochemistry, In situ hybridization
FormatReady-to-Use

Documents

Citations

Technical Information

Optimize Counterstain Intensity

The post Nuclear Fast Red Counterstain appeared first on VectorLabs.

]]> Methyl Green Counterstain https://staging.vectorlabs.com/products/vector-methyl-green-counterstain/ Wed, 26 Apr 2023 04:45:58 +0000 https://staging.vectorlabs.com/?post_type=product&p=12234 Description

Vector Methyl Green stains cell nuclei light green. It is an excellent alternative in multiple labeling when hematoxylin obscures the substrate colors.

Features:

  • Simple, two-step procedure
  • Suitable for use with a wide range of enzyme substrates
  • Suitable for use with non-aqueous mountants

Specifications

Unit Size 500 ml
Applications Immunohistochemistry / Immunocytochemistry, In situ hybridization
Format Ready-to-Use

The post Methyl Green Counterstain appeared first on VectorLabs.

]]>

Description

Vector Methyl Green stains cell nuclei light green. It is an excellent alternative in multiple labeling when hematoxylin obscures the substrate colors.

Features:

  • Simple, two-step procedure
  • Suitable for use with a wide range of enzyme substrates
  • Suitable for use with non-aqueous mountants

Specifications

Unit Size500 ml
ApplicationsImmunohistochemistry / Immunocytochemistry, In situ hybridization
FormatReady-to-Use

Documents

Product FAQs

Methyl Green counterstain does take a little bit of optimization in some applications. We would suggest heating a volume of the counterstain (~300 ml) to 60 °C and add to a Coplin jar or similar glass staining rack, and submerse the slides. This approach facilitates a better uptake of stain into the section compared with placing the slide on a heated surface and placing drops of stain onto the section. Following the incubation time (~3-5 min), remove slides and wash in tap water. Omit the acetone, acetic acid rinse step described in the instructions and move the slides directly into 95% ethanol for the dehydration, clearing and mounting process. This methodology will retain more stain in the nuclei and hence produce greater intensity.

Citations

Technical Information

Counterstain/Substrate Compatibility Table

This table is designed as a reference to determine the optimal counterstain/substrate combination for your application.

cstaincomp jpg

Optimize Counterstain Intensity

The post Methyl Green Counterstain appeared first on VectorLabs.

]]> Hematoxylin Counterstain https://staging.vectorlabs.com/products/vector-hematoxylin-counterstain/ Wed, 26 Apr 2023 04:39:20 +0000 https://staging.vectorlabs.com/?post_type=product&p=12220 Description

Vector Hematoxylin is based on Gill’s formulation, it produces blue stained nuclei with crisp detail.

Features:

  • Alcohol and mercury free
  • Progressive stain formula
  • Provides excellent color contrast with most commonly used peroxidase and alkaline phosphatase substrates
  • Suitable for use with non-aqueous and aqueous mountants

Specifications

Unit Size 500 ml
Applications Immunohistochemistry / Immunocytochemistry, In situ hybridization
Format Ready-to-Use

The post Hematoxylin Counterstain appeared first on VectorLabs.

]]>

Description

Vector Hematoxylin is based on Gill’s formulation, it produces blue stained nuclei with crisp detail.

Features:

  • Alcohol and mercury free
  • Progressive stain formula
  • Provides excellent color contrast with most commonly used peroxidase and alkaline phosphatase substrates
  • Suitable for use with non-aqueous and aqueous mountants

Specifications

Unit Size500 ml
ApplicationsImmunohistochemistry / Immunocytochemistry, In situ hybridization
FormatReady-to-Use

Documents

Citations

Technical Information

Counterstain/Substrate Compatibility Table

This table is designed as a reference to determine the optimal counterstain/substrate combination for your application.

cstaincomp jpg

Optimize Counterstain Intensity

The post Hematoxylin Counterstain appeared first on VectorLabs.

]]> ImmEdge® Hydrophobic Barrier PAP Pen https://staging.vectorlabs.com/products/immedge-hydrophobic-barrier-pen/ Wed, 12 Apr 2023 17:44:52 +0000 https://staging.vectorlabs.com/?post_type=product&p=9686 Description

The ImmEdge pen (H-4000) is an improvement over the PAP pen for tissue specimen preparation. Its hydrophobic barrier feature provides a stable, water-repellent barrier that keeps reagents localized on tissue specimens, preventing reagent mixing when multiple sections are mounted on the same slide.

The barrel of the ImmEdge Pen is composed of aluminum which allows for consistent tip retention, easier tip saturation, and improved fluid flow.

ImmEdge Hydrophobic PAP Barrier Pen Features:

  • Stable for use with buffers with and without detergent (Tween 20, Triton X-100, etc.)
  • Light blue barrier compatible with enzyme or fluorescence-based detection systems
  • Soluble in all commonly used clearing agents
  • Environmentally friendly – does not contain “ozone-depleting” substances as identified by the Montreal Protocol or the Council of the European Union

Specifications

 
Unit Size 2 pens
Applications Immunohistochemistry / Immunocytochemistry, Immunofluorescence, In situ hybridization
Safety Title WARNING: Cancer and Reproductive Harm – www.P65Warnings.ca.gov

The post ImmEdge® Hydrophobic Barrier PAP Pen appeared first on VectorLabs.

]]>

Description

The ImmEdge pen (H-4000) is an improvement over the PAP pen for tissue specimen preparation. Its hydrophobic barrier feature provides a stable, water-repellent barrier that keeps reagents localized on tissue specimens, preventing reagent mixing when multiple sections are mounted on the same slide.

The barrel of the ImmEdge Pen is composed of aluminum which allows for consistent tip retention, easier tip saturation, and improved fluid flow.

ImmEdge Hydrophobic PAP Barrier Pen Features:

  • Stable for use with buffers with and without detergent (Tween 20, Triton X-100, etc.)
  • Light blue barrier compatible with enzyme or fluorescence-based detection systems
  • Soluble in all commonly used clearing agents
  • Environmentally friendly – does not contain “ozone-depleting” substances as identified by the Montreal Protocol or the Council of the European Union

Specifications

 
Unit Size2 pens
ApplicationsImmunohistochemistry / Immunocytochemistry, Immunofluorescence, In situ hybridization
Safety TitleWARNING: Cancer and Reproductive Harm – www.P65Warnings.ca.gov

Documents

Product FAQs

The volume of the ImmEdge Pen (H-4000) is about 6 mL. We have not characterized how many times it can be used or overall length of the barrier due to variability in its application.

The nib provided with each ImmEdge Pen is 3 mm wide.

Yes. The ImmEdge Pen residue contains a wax constituent that allows for application to microscope slides that have buffer or similar aqueous solutions on the surface. Applying the pen residue to wet slides does not affect adhesion of the residue nor interfere with the tissue section.

Yes, the pen residue does fluoresce. However, this property does not limit the pens use to only light microscope applications. We routinely use the ImmEdge pen for immunofluorescent tissue staining applications. Usually the pen residue is applied well outside of the tissue section perimeter, and as such, the inherent fluorescent properties of the pen residue do not interfere with specific fluorescent signal.

Yes. The pen design ensures the nib can move and that the nib is not adhered to the pen itself. The nib needs to move so that once received the nib can be depressed to break the internal membrane to allow residue to flood the nib. In some instances, during shipping, the nib may become dislodged and fall out. Simply put the nib back into the neck of the pen and use as per the supplied instructions. Once the residue floods the nib, the nib material expands slightly to hold the nib within the pen neck.

The pen residue does contain a solvent that dries within seconds of being placed onto the slide. There is no requirement to wait for the residue to dry. In essence the residue dries the moment it is applied.

Citations

Technical Information

Check out the video below on how to use a hydrophobic barrier pen.

Resources

The post ImmEdge® Hydrophobic Barrier PAP Pen appeared first on VectorLabs.

]]>