Glucose – VectorLabs https://staging.vectorlabs.com From linker design and synthesis, bioconjugated design and manufacturing, biomolecule labeling and functionalization, and detection system and solutions, our team is here to help you move forward, with impact. Wed, 22 Oct 2025 17:14:43 +0000 en-US hourly 1 https://wordpress.org/?v=6.9 https://staging.vectorlabs.com/wp-content/uploads/2024/11/V-favicon-02-100x100.png Glucose – VectorLabs https://staging.vectorlabs.com 32 32 Lens Culinaris Agglutinin (LCA), DyLight® 649 https://staging.vectorlabs.com/products/dylight-649-labeled-lens-culinaris-agglutinin/ Tue, 02 May 2023 16:04:48 +0000 https://staging.vectorlabs.com/?post_type=product&p=14318 Description

Lens culinaris agglutinin (LCA) recognizes sequences containing α-linked mannose residues but recognizes additional sugars as part of the receptor structure, giving it a narrower specificity than Con A. An α-linked fucose residue attached to the N-acetylchitobiose portion of the core oligosaccharide significantly enhances affinity. By exploiting this increased specificity, glycoproteins and glycopeptides can be subfractionated with LCA after initial isolation with Con A.

DyLight™ 649 labeled LCA has an appropriate number of bound fluorochromes, which provides the optimum staining characteristics for this lectin. This conjugate is supplied essentially free of unconjugated fluorochromes.

  • Excitation maximum: 655 nm
  • Emission maximum: 670 nm
  • Color: Far red

 

Lens Culinaris Agglutinin (LCA), DyLight™ 649 is provided in a 1 mL aliquot at a concentration of 1 mg active conjugate/ml.

Specifications

Unit Size 1 mg
Applications Immunofluorescence, Glycobiology
Recommended Usage The recommended concentration range for use is 5-20 µg/ml. If a precipitate forms upon long-term storage, warm to 37 ºC.
Recommended Storage 2-8 °C
Maximum Excitation 655 nm
Maximum Emission 670 nm
Solution 10 mM HEPES, 0.15 M NaCl, pH 7.5, 0.08% sodium azide, 0.1 mM CaCl2 , 0.01 mM MnCl2
Concentration 1 mg active conjugate/ml
Conjugate DyLight 649
Color of Fluorescence Far Red
Sugar Specificity Mannose, Glucose

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Description

Lens culinaris agglutinin (LCA) recognizes sequences containing α-linked mannose residues but recognizes additional sugars as part of the receptor structure, giving it a narrower specificity than Con A. An α-linked fucose residue attached to the N-acetylchitobiose portion of the core oligosaccharide significantly enhances affinity. By exploiting this increased specificity, glycoproteins and glycopeptides can be subfractionated with LCA after initial isolation with Con A.

DyLight™ 649 labeled LCA has an appropriate number of bound fluorochromes, which provides the optimum staining characteristics for this lectin. This conjugate is supplied essentially free of unconjugated fluorochromes.

  • Excitation maximum: 655 nm
  • Emission maximum: 670 nm
  • Color: Far red

Lens Culinaris Agglutinin (LCA), DyLight™ 649 is provided in a 1 mL aliquot at a concentration of 1 mg active conjugate/ml.

Specifications

Unit Size1 mg
ApplicationsImmunofluorescence, Glycobiology
Recommended UsageThe recommended concentration range for use is 5-20 µg/ml. If a precipitate forms upon long-term storage, warm to 37 ºC.
Recommended Storage2-8 °C
Maximum Excitation655 nm
Maximum Emission670 nm
Solution10 mM HEPES, 0.15 M NaCl, pH 7.5, 0.08% sodium azide, 0.1 mM CaCl2 , 0.01 mM MnCl2
Concentration1 mg active conjugate/ml
ConjugateDyLight 649
Color of FluorescenceFar Red
Sugar SpecificityMannose, Glucose

Documents

Citations

Technical Information

Lens culinaris agglutinin is composed of four subunits – two of about 17 kDa and two of 8 kDa. 

LCA has been found to be one of the most effective agents in preventing skin allograft rejection in model systems. LCA has been used to purify numerous glycoproteins, including immunoglobulins, histocompatibility antigens, and α2-macroglobulin.

Accompanying each fluorescent lectin is an analysis data sheet summarizing the results of our quality control tests and providing pertinent information on the product. All of these reagents are supplied as solutions preserved with sodium azide. 

Inhibiting/Eluting Sugar: mixture of 200 mM α-methylmannoside/200 mM α-methylglucoside

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]]> Lens Culinaris Agglutinin (LCA), Unconjugated https://staging.vectorlabs.com/products/unconjugated-lens-culinaris-agglutinin/ Tue, 02 May 2023 14:31:38 +0000 https://staging.vectorlabs.com/?post_type=product&p=14116 Description

LCA recognizes sequences containing α-linked mannose residues but recognizes additional sugars as part of the receptor structure, giving it a narrower specificity than Con A. An α-linked fucose residue attached to the N-acetylchitobiose portion of the core oligosaccharide significantly enhances affinity. By exploiting this narrower specificity, glycoproteins and glycopeptides can be subfractionated with LCA after initial isolation with Con A.

Specifications

 
Unit Size 10 mg, 25 mg
Applications Immunohistochemistry / Immunocytochemistry, Immunofluorescence, Blotting Applications, Glycobiology, Mitogenic Stimulation
Recommended Usage Although many buffers can be employed for reconstituting and diluting this lectin, 10 mM HEPES buffered saline, pH 8.5, 0.1 mM CaCl2 is recommended. For preserving solutions stored at 4 ºC, 0.08% sodium azide can be used.
Recommended Storage 2-8 °C
Inhibiting and/or Eluting Sugar mixture of 200 mM α−methylmannoside/200 mM α−methylglucoside
Conjugate Unconjugated
Sugar Specificity Mannose, Glucose

The post Lens Culinaris Agglutinin (LCA), Unconjugated appeared first on VectorLabs.

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Description

LCA recognizes sequences containing α-linked mannose residues but recognizes additional sugars as part of the receptor structure, giving it a narrower specificity than Con A. An α-linked fucose residue attached to the N-acetylchitobiose portion of the core oligosaccharide significantly enhances affinity. By exploiting this narrower specificity, glycoproteins and glycopeptides can be subfractionated with LCA after initial isolation with Con A.

Specifications

 
Unit Size10 mg, 25 mg
ApplicationsImmunohistochemistry / Immunocytochemistry, Immunofluorescence, Blotting Applications, Glycobiology, Mitogenic Stimulation
Recommended UsageAlthough many buffers can be employed for reconstituting and diluting this lectin, 10 mM HEPES buffered saline, pH 8.5, 0.1 mM CaCl2 is recommended. For preserving solutions stored at 4 ºC, 0.08% sodium azide can be used.
Recommended Storage2-8 °C
Inhibiting and/or Eluting Sugarmixture of 200 mM α−methylmannoside/200 mM α−methylglucoside
ConjugateUnconjugated
Sugar SpecificityMannose, Glucose

Documents

Citations

Technical Information

Lens culinaris agglutinin is composed of four subunits – two of about 17 kDa and two of 8 kDa. LCA has been found to be one of the most effective agents in preventing skin allograft rejection in model systems. LCA has been used to purify numerous glycoproteins, including immunoglobulins, histocompatibility antigens, and α2-macroglobulin.

Inhibiting/Eluting Sugar: mixture of 200 mM α-methylmannoside/200 mM α-methylglucoside

The post Lens Culinaris Agglutinin (LCA), Unconjugated appeared first on VectorLabs.

]]> Concanavalin A (Con A), Agarose bound https://staging.vectorlabs.com/products/agarose-bound-concanavalin-a-con-a/ Tue, 02 May 2023 02:40:58 +0000 https://staging.vectorlabs.com/?post_type=product&p=13951

Description

Agarose bound Con A is prepared using our affinity-purified lectins. Con A recognizes α-linked mannose present as part of a core oligosaccharide in many serum and membrane glycoproteins. At neutral and alkaline pH, Con A exists as a tetramer of four identical subunits; below pH 5.6, Con A dissociates into active dimers of 52 kDa. Acetylation, succinylation, or other derivatizations can also produce stable forms with dimeric structures. (See succinylated Con A). Nicks in the sequence are often present in the purest preparations due to hydrolytic damage within the seeds.

Features:

  • Bead diameter ranges in size from 45-165 microns
  • Matrix is stable in solutions at pH 3-11 as well as many organic solvents
  • Immobilized lectins are prepared using affinity purified lectins
  • Covalent attachment preserves lectin activity and minimizes conformational changes that might result in nonspecific or hydrophobic interactions
  • Hydrophilic spacer arm is inserted between the lectin and the matrix
  • Conjugated proteins are not leached off the beads by Tris or other routinely used buffers
  • No residual charges present after conjugation.  This minimizes non-specific binding to the matrix
  • Product supplied as a 1:1 suspension in buffer

Specifications

Unit Size 10 ml, 100 ml
Applications Glycobiology, Affinity Chromatography
Recommended Storage 2-8 °C DO NOT FREEZE
Solution 10 mM HEPES, pH 7.5, 0.15 M NaCl, 0.1 mM CaCl2, 0.01 mM MnCl2, 20 mM glucose, 0.08% sodium azide
Recommended Usage Wash gel thoroughly with buffer before use to remove sugar added to stabilize the lectin. Recommended product for eluting glycoconjugates bound to this agarose-lectin: Glycoprotein Eluting Solution, Cat. No. ES-1100. Alternatively, a 0.2 M α-methyl mannoside/0.2 M α-methyl glucoside mixture can be used. After use, wash the gel with several column volumes of buffered saline, then resuspend gel in buffered saline containing 0.08% sodium azide for storage.
Matrix Conjugate Lectins
Sugar Specificity Mannose, Glucose
Conjugate Agarose

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Description

Agarose bound Con A is prepared using our affinity-purified lectins. Con A recognizes α-linked mannose present as part of a core oligosaccharide in many serum and membrane glycoproteins. At neutral and alkaline pH, Con A exists as a tetramer of four identical subunits; below pH 5.6, Con A dissociates into active dimers of 52 kDa. Acetylation, succinylation, or other derivatizations can also produce stable forms with dimeric structures. (See succinylated Con A). Nicks in the sequence are often present in the purest preparations due to hydrolytic damage within the seeds.

Features:

  • Bead diameter ranges in size from 45-165 microns
  • Matrix is stable in solutions at pH 3-11 as well as many organic solvents
  • Immobilized lectins are prepared using affinity purified lectins
  • Covalent attachment preserves lectin activity and minimizes conformational changes that might result in nonspecific or hydrophobic interactions
  • Hydrophilic spacer arm is inserted between the lectin and the matrix
  • Conjugated proteins are not leached off the beads by Tris or other routinely used buffers
  • No residual charges present after conjugation.  This minimizes non-specific binding to the matrix
  • Product supplied as a 1:1 suspension in buffer

Specifications

Unit Size10 ml, 100 ml
ApplicationsGlycobiology, Affinity Chromatography
Recommended Storage2-8 °C DO NOT FREEZE
Solution10 mM HEPES, pH 7.5, 0.15 M NaCl, 0.1 mM CaCl2, 0.01 mM MnCl2, 20 mM glucose, 0.08% sodium azide
Recommended UsageWash gel thoroughly with buffer before use to remove sugar added to stabilize the lectin. Recommended product for eluting glycoconjugates bound to this agarose-lectin: Glycoprotein Eluting Solution, Cat. No. ES-1100. Alternatively, a 0.2 M α-methyl mannoside/0.2 M α-methyl glucoside mixture can be used. After use, wash the gel with several column volumes of buffered saline, then resuspend gel in buffered saline containing 0.08% sodium azide for storage.
Matrix ConjugateLectins
Sugar SpecificityMannose, Glucose
ConjugateAgarose

Documents

Product FAQs

Our agarose lectin products are supplied as hydrated matrix solutions in amber glass bottles. The agarose (bead) material will settle and you will see two phases in the tube supplied. The upper phase is buffer. A column can be prepared in a commercial plastic device such as Bio-Rad Cat # 732-6008 or an inverted Pasteur pipet with glass wool lightly packed in the neck to retain the agarose. 1) Draw (pipet) the desired amount of settled agarose-lectin (gel) from the stock bottle into the prepared column and let the buffer drain by gravity.(Sometimes an air bubble in the column tip prevents flow; tapping the column should get the flow started). 2) Wash the gel with 10 column volumes of buffer, such as HBS (10 mM HEPES, 0.15 M NaCl, pH 7.5) and discard the flow through. 3) Place a collection vessel (e.g. glass test tube) under the column tip and apply the glycoprotein-containing solution.Allow the solution to drain through using gravity. We recommend against pushing or pulling the material through the column. Retain the flow through material until the desired binding has been confirmed. 4) After sample application, wash column with 2-3 column volumes of buffer (or until the absorbance at 280nm is reduced to a satisfactory level) to remove unbound materials before elution. 5) Place a fresh collection vessel under the column tip.Apply the eluting solution again letting gravity do the work of moving the solution over the column. Note that in some cases, several column volumes of eluting solution may be required to achieved adequate release of bound material. 6) Following elution, the column can be prepared for reuse by washing with 10 column volumes of buffer. 7) If the column is to be stored, equilibrate the column with buffer containing 0.08% sodium azide. Cover the column with a plastic wrap, or similar, to prevent desiccation and keep at 4 degrees Celsius. The column will be stable for many months when stored under these conditions.

The pH should be near neutral, the maximum pressure for packing the resin is 10 psi, and the maximum flow rate 3.5 ml/min.

Citations

Technical Information

Con A requires calcium or manganese ions at each of its four saccharide binding sites. Although these divalent metal ions are bound tightly to the polypeptide structure, buffers which can bind calcium (such as phosphate) generally should be avoided in diluting Con A, since a gradual loss in activity may occur.

Agarose bound* Con A is prepared using our affinity-purified lectins. Heat stable, cross-linked 4% agarose beads with a molecular weight exclusion limit of about 2×107 daltons are used as the solid-phase matrix to which the lectins are covalently coupled. 

This coupling method provides several advantages over the traditional cyanogen bromide procedure:

  • Maximum carbohydrate binding activity of the coupled lectins is retained
  • Linkage is stable over a range of pH values

Our agarose bound lectins are supplied at a constant concentration of lectin per ml of settled beads. The concentration for each lectin is selected to achieve the highest glycoconjugate binding capacity per mg of lectin present in the beads. Each lot is tested for its binding capacity using glycoproteins known to bind the lectin. This provides a guideline for the user and assures the quality of our agarose bound lectins.

Inhibiting/Eluting Sugar: mixture of 200 mM α-methylmannoside/200 mM α-methylglucoside or Glycoprotein Eluting Solution (ES-1100)

*6 mg lectin/ml gel

The post Concanavalin A (Con A), Agarose bound appeared first on VectorLabs.

]]> Concanavalin A (Con A), Rhodamine https://staging.vectorlabs.com/products/rhodamine-concanavalin-a-con-a/ Fri, 28 Apr 2023 19:55:21 +0000 https://staging.vectorlabs.com/?post_type=product&p=12848 Description

Con A recognizes α-linked mannose present as part of a “core oligosaccharide” in many serum and membrane glycoproteins.

Rhodamine labeled Con A has an appropriate number of fluorochromes bound to provide the optimum staining characteristics for this lectin. This conjugate is supplied essentially free of unconjugated fluorochromes. The excitation maximum is at 550 nm and the emission maximum is at 575 nm.

Specifications

Unit Size 25 mg
Applications Immunofluorescence, Glycobiology
Recommended Usage The recommended concentration range for use is 5-20 µg/ml.
Recommended Storage 2-8 °C
Maximum Excitation 545-555 nm
Maximum Emission 570-580 nm
Solution 10 mM HEPES, 0.15 M NaCl, pH 7.5 0.08% sodium azide, 0.1 mM CaCl2, 0.01 mM MnCl2, 5 mM mannose
Concentration 5 mg active conjugate/ml
Conjugate Rhodamine
Color of Fluorescence Red
Sugar Specificity Mannose, Glucose

The post Concanavalin A (Con A), Rhodamine appeared first on VectorLabs.

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Description

Con A recognizes α-linked mannose present as part of a “core oligosaccharide” in many serum and membrane glycoproteins.

Rhodamine labeled Con A has an appropriate number of fluorochromes bound to provide the optimum staining characteristics for this lectin. This conjugate is supplied essentially free of unconjugated fluorochromes. The excitation maximum is at 550 nm and the emission maximum is at 575 nm.

Specifications

Unit Size25 mg
ApplicationsImmunofluorescence, Glycobiology
Recommended UsageThe recommended concentration range for use is 5-20 µg/ml.
Recommended Storage2-8 °C
Maximum Excitation545-555 nm
Maximum Emission570-580 nm
Solution10 mM HEPES, 0.15 M NaCl, pH 7.5 0.08% sodium azide, 0.1 mM CaCl2, 0.01 mM MnCl2, 5 mM mannose
Concentration5 mg active conjugate/ml
ConjugateRhodamine
Color of FluorescenceRed
Sugar SpecificityMannose, Glucose

Documents

Citations

Technical Information

At neutral and alkaline pH, Con A exists as a tetramer of four identical subunits; below pH 5.6, Con A dissociates into active dimers of 52 kDa. Acetylation, succinylation, or other derivatizations can also produce stable forms with dimeric structures. (See succinylated Con A). Nicks in the sequence are often present in the purest preparations due to hydrolytic damage within the seeds.

Con A requires calcium or manganese ions at each of its four saccharide binding sites. Although these divalent metal ions are bound tightly to the polypeptide structure, buffers which can bind calcium (such as phosphate) generally should be avoided in diluting Con A, since a gradual loss in activity may occur.

Accompanying each fluorescent lectin is an analysis data sheet summarizing the results of our quality control tests and providing pertinent information on the product. All of these reagents are supplied as solutions preserved with sodium azide.

Inhibiting/Eluting Sugar: mixture of 200 mM α-methylmannoside/200 mM α-methylglucoside

The post Concanavalin A (Con A), Rhodamine appeared first on VectorLabs.

]]> Pisum Sativum Agglutinin (PSA), Unconjugated https://staging.vectorlabs.com/products/unconj-pisum-sativum-agglutinin-psa/ Thu, 27 Apr 2023 14:05:38 +0000 https://staging.vectorlabs.com/?post_type=product&p=12368 Description

Pisum sativum agglutinin is nearly identical in structure and carbohydrate specificity to Lens culinaris agglutinin. The lectin has specificity toward α-linked mannose-containing oligosaccharides, with an N-acetylchitobiose-linked α-fucose residue included in the receptor sequence. Calcium and manganese ions are required for activity.

Specifications

Unit Size 10 mg
Applications Immunohistochemistry / Immunocytochemistry, Immunofluorescence, Blotting Applications, Glycobiology, Mitogenic Stimulation
Recommended Usage Although many buffers can be employed for reconstituting and diluting this lectin, 10 mM HEPES buffered saline, pH 8.5, 0.1 mM CaCl2 is recommended. For preserving solutions stored at 4 ºC, 0.08% sodium azide can be used.
Recommended Storage 2-8 °C
Conjugate Unconjugated
Sugar Specificity Mannose, Glucose

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Description

Pisum sativum agglutinin is nearly identical in structure and carbohydrate specificity to Lens culinaris agglutinin. The lectin has specificity toward α-linked mannose-containing oligosaccharides, with an N-acetylchitobiose-linked α-fucose residue included in the receptor sequence. Calcium and manganese ions are required for activity.

Specifications

Unit Size10 mg
ApplicationsImmunohistochemistry / Immunocytochemistry, Immunofluorescence, Blotting Applications, Glycobiology, Mitogenic Stimulation
Recommended UsageAlthough many buffers can be employed for reconstituting and diluting this lectin, 10 mM HEPES buffered saline, pH 8.5, 0.1 mM CaCl2 is recommended. For preserving solutions stored at 4 ºC, 0.08% sodium azide can be used.
Recommended Storage2-8 °C
ConjugateUnconjugated
Sugar SpecificityMannose, Glucose

Documents

Citations

Technical Information

PSA has been used to fractionate cells, to isolate glycoproteins and glycopeptides, to distinguish between normal and virally transformed cells, as a T-cell mitogen, and as an inhibitor of allograft rejection.

Inhibiting/Eluting Sugar: mixture of 200 mM α-methylmannoside/200 mM α-methylglucoside

The post Pisum Sativum Agglutinin (PSA), Unconjugated appeared first on VectorLabs.

]]> Concanavalin A (Con A), Unconjugated https://staging.vectorlabs.com/products/unconj-concanavalin-a-con-a/ Thu, 27 Apr 2023 13:58:28 +0000 https://staging.vectorlabs.com/?post_type=product&p=12353 Description

Con A recognizes α-linked mannose present as part of a “core oligosaccharide” in many serum and membrane glycoproteins.

Specifications

More Information
Unit Size 500 mg
Applications Immunohistochemistry / Immunocytochemistry, Immunofluorescence, Blotting Applications, Glycobiology, Mitogenic Stimulation
Recommended Usage Although many buffers can be employed for reconstituting and diluting this lectin, 10 mM HEPES buffered saline, pH 8.5, 0.1 mM CaCl2 is recommended. For preserving solutions stored at 4 ºC, 0.08% sodium azide can be used.
Conjugate Unconjugated
Sugar Specificity Mannose, Glucose

The post Concanavalin A (Con A), Unconjugated appeared first on VectorLabs.

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Description

Con A recognizes α-linked mannose present as part of a “core oligosaccharide” in many serum and membrane glycoproteins.

Specifications

More Information
Unit Size500 mg
ApplicationsImmunohistochemistry / Immunocytochemistry, Immunofluorescence, Blotting Applications, Glycobiology, Mitogenic Stimulation
Recommended UsageAlthough many buffers can be employed for reconstituting and diluting this lectin, 10 mM HEPES buffered saline, pH 8.5, 0.1 mM CaCl2 is recommended. For preserving solutions stored at 4 ºC, 0.08% sodium azide can be used.
ConjugateUnconjugated
Sugar SpecificityMannose, Glucose

Documents

Citations

Technical Information

Con A recognizes α-linked mannose present as part of a core oligosaccharide in many serum and membrane glycoproteins. At neutral and alkaline pH, Con A exists as a tetramer of four identical subunits; below pH 5.6, Con A dissociates into active dimers of 52 kDa. Acetylation, succinylation, or other derivatizations can also produce stable forms with dimeric structures. (See succinylated Con A). Nicks in the sequence are often present in the purest preparations due to hydrolytic damage within the seeds.

Con A requires calcium or manganese ions at each of its four saccharide binding sites. Although these divalent metal ions are bound tightly to the polypeptide structure, buffers which can bind calcium (such as phosphate) generally should be avoided in diluting Con A, since a gradual loss in activity may occur.

Inhibiting/Eluting Sugar: mixture of 200 mM α-methylmannoside/200 mM α-methylglucoside

The post Concanavalin A (Con A), Unconjugated appeared first on VectorLabs.

]]> Lens Culinaris Agglutinin (LCA), Fluorescein https://staging.vectorlabs.com/products/fluorescein-lens-culinaris-agglutinin-lca/ Tue, 25 Apr 2023 18:37:36 +0000 https://staging.vectorlabs.com/?post_type=product&p=11886 Description

LCA recognizes sequences containing α-linked mannose residues but recognizes additional sugars as part of the receptor structure, giving it a narrower specificity than Con A. An α-linked fucose residue attached to the N-acetylchitobiose portion of the core oligosaccharide significantly enhances affinity. By exploiting this narrower specificity, glycoproteins and glycopeptides can be subfractionated with LCA after initial isolation with Con A.

Fluorescein labeled LCA has an appropriate number of fluorochromes bound to provide the optimum staining characteristics for this lectin. This conjugate is supplied essentially free of unconjugated fluorochromes. The excitation maximum is at 495 nm and the emission maximum is at 515 nm.

Specifications

Unit Size 5 mg
Applications Immunofluorescence, Glycobiology
Recommended Usage The recommended concentration range for use is 5-20 µg/ml.
Recommended Storage 2-8 °C
Maximum Excitation 495-500 nm
Maximum Emission 514-521 nm
Solution 10 mM HEPES, 0.15 M NaCl, pH 7.5, 0.08% sodium azide, 0.1 mM CaCl2, 0.01 mM MnCl2.
Concentration 5 mg active conjugate/ml
Conjugate Fluorescein
Color of Fluorescence Green
Sugar Specificity Mannose, Glucose

The post Lens Culinaris Agglutinin (LCA), Fluorescein appeared first on VectorLabs.

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Description

LCA recognizes sequences containing α-linked mannose residues but recognizes additional sugars as part of the receptor structure, giving it a narrower specificity than Con A. An α-linked fucose residue attached to the N-acetylchitobiose portion of the core oligosaccharide significantly enhances affinity. By exploiting this narrower specificity, glycoproteins and glycopeptides can be subfractionated with LCA after initial isolation with Con A.

Fluorescein labeled LCA has an appropriate number of fluorochromes bound to provide the optimum staining characteristics for this lectin. This conjugate is supplied essentially free of unconjugated fluorochromes. The excitation maximum is at 495 nm and the emission maximum is at 515 nm.

Specifications

Unit Size5 mg
ApplicationsImmunofluorescence, Glycobiology
Recommended UsageThe recommended concentration range for use is 5-20 µg/ml.
Recommended Storage2-8 °C
Maximum Excitation495-500 nm
Maximum Emission514-521 nm
Solution10 mM HEPES, 0.15 M NaCl, pH 7.5, 0.08% sodium azide, 0.1 mM CaCl2, 0.01 mM MnCl2.
Concentration5 mg active conjugate/ml
ConjugateFluorescein
Color of FluorescenceGreen
Sugar SpecificityMannose, Glucose

Documents

Citations

Technical Information

Lens culinaris agglutinin is composed of four subunits – two of about 17 kDa and two of 8 kDa. LCA has been found to be one of the most effective agents in preventing skin allograft rejection in model systems. LCA has been used to purify numerous glycoproteins, including immunoglobulins, histocompatibility antigens, and α2-macroglobulin.

Accompanying each fluorescent lectin is an analysis data sheet summarizing the results of our quality control tests and providing pertinent information on the product. All of these reagents are supplied as solutions preserved with sodium azide. 

Inhibiting/Eluting Sugar: mixture of 200 mM α-methylmannoside/200 mM α-methylglucoside

The post Lens Culinaris Agglutinin (LCA), Fluorescein appeared first on VectorLabs.

]]> Concanavalin A (Con A), Fluorescein https://staging.vectorlabs.com/products/fluorescein-concanavalin-a-con-a/ Tue, 25 Apr 2023 16:24:12 +0000 https://staging.vectorlabs.com/?post_type=product&p=11865 Description

Con A recognizes α-linked mannose present as part of a “core oligosaccharide” in many serum and membrane glycoproteins.

Fluorescein labeled Con A has an appropriate number of fluorochromes bound to provide the optimum staining characteristics for this lectin. This conjugate is supplied essentially free of unconjugated fluorochromes.

  • Excitation maximum: 495 nm
  • Emission maximum: 515 nm
  • Color: Green

Specifications

Unit Size 25 mg
Applications Immunofluorescence, Glycobiology
Recommended Usage The recommended concentration range for use is 5-20 µg/ml.
Recommended Storage 2-8 °C
Maximum Excitation 495-500 nm
Maximum Emission 514-521 nm
Solution 10 mM HEPES, 0.15 M NaCl, pH 7.5, 0.08% sodium azide, 0.1 mM CaCl2, 0.01 mM MnCl2.
Concentration 5 mg active conjugate/ml
Conjugate Fluorescein
Color of Fluorescence Green
Sugar Specificity Mannose, Glucose

The post Concanavalin A (Con A), Fluorescein appeared first on VectorLabs.

]]>

Description

Con A recognizes α-linked mannose present as part of a “core oligosaccharide” in many serum and membrane glycoproteins. 

Fluorescein labeled Con A has an appropriate number of fluorochromes bound to provide the optimum staining characteristics for this lectin. This conjugate is supplied essentially free of unconjugated fluorochromes. 

  • Excitation maximum: 495 nm
  • Emission maximum: 515 nm
  • Color: Green

Specifications

Unit Size25 mg
ApplicationsImmunofluorescence, Glycobiology
Recommended UsageThe recommended concentration range for use is 5-20 µg/ml.
Recommended Storage2-8 °C
Maximum Excitation495-500 nm
Maximum Emission514-521 nm
Solution10 mM HEPES, 0.15 M NaCl, pH 7.5, 0.08% sodium azide, 0.1 mM CaCl2, 0.01 mM MnCl2.
Concentration5 mg active conjugate/ml
ConjugateFluorescein
Color of FluorescenceGreen
Sugar SpecificityMannose, Glucose

Documents

Citations

Technical Information

At neutral and alkaline pH, Con A exists as a tetramer of four identical subunits; below pH 5.6, Con A dissociates into active dimers of 52 kDa. Acetylation, succinylation, or other derivatizations can also produce stable forms with dimeric structures. (See succinylated Con A). Nicks in the sequence are often present in the purest preparations due to hydrolytic damage within the seeds.
 

Con A requires calcium or manganese ions at each of its four saccharide binding sites. Although these divalent metal ions are bound tightly to the polypeptide structure, buffers which can bind calcium (such as phosphate) generally should be avoided in diluting Con A, since a gradual loss in activity may occur.

Accompanying each fluorescent lectin is an analysis data sheet summarizing the results of our quality control tests and providing pertinent information on the product. All of these reagents are supplied as solutions preserved with sodium azide.

Inhibiting/Eluting Sugar: mixture of 200 mM α-methylmannoside/200 mM α-methylglucoside

The post Concanavalin A (Con A), Fluorescein appeared first on VectorLabs.

]]> Pisum Sativum Agglutinin (PSA), Biotinylated https://staging.vectorlabs.com/products/biotinylated-pisum-sativum-agglutinin-psa/ Wed, 19 Apr 2023 17:36:28 +0000 https://staging.vectorlabs.com/?post_type=product&p=10932 Description

The lectin has specificity toward α-linked mannose-containing oligosaccharides, with an N-acetylchitobiose-linked α-fucose residue included in the receptor sequence.

Biotinylated Pisum sativum agglutinin has an appropriate number of biotins bound to provide the optimum staining characteristics for this lectin. This conjugate is supplied essentially free of unconjugated biotins and is preserved with sodium azide.

Specifications

Unit Size 5 mg
Applications Immunohistochemistry / Immunocytochemistry, Immunofluorescence, Blotting Applications, Elispot, ELISAs, Glycobiology
Recommended Usage For most applications we recommend a freshly prepared working solution of 5-20 µg/ml in the below buffer.
Recommended Storage 2-8 °C; Store frozen for long term storage
Solution 10 mM HEPES, pH 7.5, 0.15 M NaCl, 0.1 mM CaCl2, 0.01 mM MnCl2, 0.08% sodium azide
Concentration 5 mg active conjugate/ml
Conjugate Biotinylated
Sugar Specificity Mannose, Glucose

Technical Information

Pisum sativum agglutinin is nearly identical in structure and carbohydrate specificity to Lens culinaris agglutinin. Calcium and manganese ions are required for activity. PSA has been used to fractionate cells, to isolate glycoproteins and glycopeptides, to distinguish between normal and virally transformed cells, as a T-cell mitogen, and as an inhibitor of allograft rejection.

This biotinylated lectin is an ideal intermediate for examining glycoconjugates using the Biotin-Avidin/Streptavidin System. First the biotinylated lectin is added, followed by the VECTASTAIN ABC Reagent, Avidin D conjugate, or streptavidin derivative.

Inhibiting/Eluting Sugar: mixture of 200 mM α-methylmannoside/200 mM α-methylglucoside

The post Pisum Sativum Agglutinin (PSA), Biotinylated appeared first on VectorLabs.

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Description

The lectin has specificity toward α-linked mannose-containing oligosaccharides, with an N-acetylchitobiose-linked α-fucose residue included in the receptor sequence. 

Biotinylated Pisum sativum agglutinin has an appropriate number of biotins bound to provide the optimum staining characteristics for this lectin. This conjugate is supplied essentially free of unconjugated biotins and is preserved with sodium azide.

Specifications

Unit Size5 mg
ApplicationsImmunohistochemistry / Immunocytochemistry, Immunofluorescence, Blotting Applications, Elispot, ELISAs, Glycobiology
Recommended UsageFor most applications we recommend a freshly prepared working solution of 5-20 µg/ml in the below buffer.
Recommended Storage2-8 °C; Store frozen for long term storage
Solution10 mM HEPES, pH 7.5, 0.15 M NaCl, 0.1 mM CaCl2, 0.01 mM MnCl2, 0.08% sodium azide
Concentration5 mg active conjugate/ml
ConjugateBiotinylated
Sugar SpecificityMannose, Glucose

Documents

Product FAQs

From our experience we have found that some lectins require Ca++ to be present for optimal binding activity. We suggest using calcium chloride (CaCl2) to fortify working solutions and ensure a minimum level of Ca++ is met. This may be particularly pertinent if using phosphate based buffers as diluents and storage solutions.

Citations

Technical Information

Pisum sativum agglutinin is nearly identical in structure and carbohydrate specificity to Lens culinaris agglutinin. Calcium and manganese ions are required for activity. PSA has been used to fractionate cells, to isolate glycoproteins and glycopeptides, to distinguish between normal and virally transformed cells, as a T-cell mitogen, and as an inhibitor of allograft rejection.

This biotinylated lectin is an ideal intermediate for examining glycoconjugates using the Biotin-Avidin/Streptavidin System. First the biotinylated lectin is added, followed by the VECTASTAIN ABC Reagent, Avidin D conjugate, or streptavidin derivative.

Inhibiting/Eluting Sugar: mixture of 200 mM α-methylmannoside/200 mM α-methylglucoside

The post Pisum Sativum Agglutinin (PSA), Biotinylated appeared first on VectorLabs.

]]> Lens Culinaris Agglutinin (LCA), Biotinylated https://staging.vectorlabs.com/products/biotinylated-lens-culinaris-agglutinin-lca/ Wed, 19 Apr 2023 17:30:48 +0000 https://staging.vectorlabs.com/?post_type=product&p=10925 Description

LCA recognizes sequences containing α-linked mannose residues but recognizes additional sugars as part of the receptor structure, giving it a narrower specificity than Con A. An α-linked fucose residue attached to the N-acetylchitobiose portion of the core oligosaccharide significantly enhances affinity.  By exploiting this narrower specificity, glycoproteins and glycopeptides can be subfractionated with LCA after initial isolation with Con A.

Biotinylated Lens culinaris agglutinin has an appropriate number of biotins bound to provide the optimum staining characteristics for this lectin. This conjugate is supplied essentially free of unconjugated biotins and is preserved with sodium azide.

Specifications

Unit Size 5 mg
Applications Immunohistochemistry / Immunocytochemistry, Immunofluorescence, Blotting Applications, Elispot, ELISAs, Glycobiology
Recommended Usage For most applications we recommend a freshly prepared working solution of 5-20 µg/ml in the below buffer.
Recommended Storage 2-8 °C; Store frozen for long term storage
Solution 10 mM HEPES, 0.15 M NaCl, pH 7.5, 0.1 mM CaCl2, 0.01 mM MnCl2, 0.08% sodium azide, 10 mM methyl-alpha-D-mannopyranoside.
Concentration 5 mg active conjugate/ml
Conjugate Biotinylated
Sugar Specificity Mannose, Glucose

Technical Information

Lens culinaris agglutinin is composed of four subunits – two of about 17 kDa and two of 8 kDa. LCA has been found to be one of the most effective agents in preventing skin allograft rejection in model systems. LCA has been used to purify numerous glycoproteins, including immunoglobulins, histocompatibility antigens, and α2-macroglobulin.

This biotinylated lectin is an ideal intermediate for examining glycoconjugates using the Biotin-Avidin/Streptavidin System. First the biotinylated lectin is added, followed by the VECTASTAIN ABC Reagent, Avidin D conjugate, or streptavidin derivative.

Inhibiting/Eluting Sugar: mixture of 200 mM α-methylmannoside/200 mM α-methylglucoside

The post Lens Culinaris Agglutinin (LCA), Biotinylated appeared first on VectorLabs.

]]>

Description

LCA recognizes sequences containing α-linked mannose residues but recognizes additional sugars as part of the receptor structure, giving it a narrower specificity than Con A. An α-linked fucose residue attached to the N-acetylchitobiose portion of the core oligosaccharide significantly enhances affinity.  By exploiting this narrower specificity, glycoproteins and glycopeptides can be subfractionated with LCA after initial isolation with Con A.

Biotinylated Lens culinaris agglutinin has an appropriate number of biotins bound to provide the optimum staining characteristics for this lectin. This conjugate is supplied essentially free of unconjugated biotins and is preserved with sodium azide.

Specifications

Unit Size5 mg
ApplicationsImmunohistochemistry / Immunocytochemistry, Immunofluorescence, Blotting Applications, Elispot, ELISAs, Glycobiology
Recommended UsageFor most applications we recommend a freshly prepared working solution of 5-20 µg/ml in the below buffer.
Recommended Storage2-8 °C; Store frozen for long term storage
Solution10 mM HEPES, 0.15 M NaCl, pH 7.5, 0.1 mM CaCl2, 0.01 mM MnCl2, 0.08% sodium azide, 10 mM methyl-alpha-D-mannopyranoside.
Concentration5 mg active conjugate/ml
ConjugateBiotinylated
Sugar SpecificityMannose, Glucose

Documents

Product FAQs

From our experience we have found that some lectins require Ca++ to be present for optimal binding activity. We suggest using calcium chloride (CaCl2) to fortify working solutions and ensure a minimum level of Ca++ is met. This may be particularly pertinent if using phosphate based buffers as diluents and storage solutions.

Citations

Technical Information

Lens culinaris agglutinin is composed of four subunits – two of about 17 kDa and two of 8 kDa. LCA has been found to be one of the most effective agents in preventing skin allograft rejection in model systems. LCA has been used to purify numerous glycoproteins, including immunoglobulins, histocompatibility antigens, and α2-macroglobulin.

This biotinylated lectin is an ideal intermediate for examining glycoconjugates using the Biotin-Avidin/Streptavidin System. First the biotinylated lectin is added, followed by the VECTASTAIN ABC Reagent, Avidin D conjugate, or streptavidin derivative.

Inhibiting/Eluting Sugar: mixture of 200 mM α-methylmannoside/200 mM α-methylglucoside

The post Lens Culinaris Agglutinin (LCA), Biotinylated appeared first on VectorLabs.

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