[GlcNAc]1-3 – VectorLabs https://staging.vectorlabs.com From linker design and synthesis, bioconjugated design and manufacturing, biomolecule labeling and functionalization, and detection system and solutions, our team is here to help you move forward, with impact. Wed, 22 Oct 2025 17:14:45 +0000 en-US hourly 1 https://wordpress.org/?v=6.9 https://staging.vectorlabs.com/wp-content/uploads/2024/11/V-favicon-02-100x100.png [GlcNAc]1-3 – VectorLabs https://staging.vectorlabs.com 32 32 Lycopersicon Esculentum (Tomato) Lectin (LEL, TL), DyLight® 649 https://staging.vectorlabs.com/products/dylight-649-lycopersicon-esculentum-tomato/ Tue, 02 May 2023 02:25:11 +0000 https://staging.vectorlabs.com/?post_type=product&p=13922 Description

Tomato lectin (from Lycopersicon esculentum) is an effective marker of blood vessels and microglial cells in rodents. Conjugation of the lectin with a fluorophore facilitates fast, one-step detection and visualization using intravascular perfusion methods or direct application to tissue sections.

DyLight™ 649 labeled tomato lectin has an appropriate number of fluorochromes bound to provide the optimum staining characteristics for this lectin. This conjugate is supplied essentially free of unconjugated fluorochromes.

  • Excitation maximum:  655 nm
  • Emission maximum:  670 nm
  • Color:  Far red

Specifications

Unit Size 1 mg
Applications Immunofluorescence, Glycobiology
Recommended Usage The recommended concentration range for use is 5-20 µg/ml. If a precipitate forms upon long-term storage, warm to 37 ºC.
Recommended Storage 2-8 °C
Maximum Excitation 655 nm
Maximum Emission 670 nm
Solution 10 mM HEPES, 0.15 M NaCl, pH 7.5, 0.08% sodium azide, 0.1 mM CaCl2.
Concentration 1 mg active conjugate/ml
Conjugate DyLight 649
Color of Fluorescence Far Red
Sugar Specificity [GlcNAc]1-3, N-Acetylglucosamine

The post Lycopersicon Esculentum (Tomato) Lectin (LEL, TL), DyLight® 649 appeared first on VectorLabs.

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Description

Tomato lectin (from Lycopersicon esculentum) is an effective marker of blood vessels and microglial cells in rodents. Conjugation of the lectin with a fluorophore facilitates fast, one-step detection and visualization using intravascular perfusion methods or direct application to tissue sections.

DyLight™ 649 labeled tomato lectin has an appropriate number of fluorochromes bound to provide the optimum staining characteristics for this lectin. This conjugate is supplied essentially free of unconjugated fluorochromes.

  • Excitation maximum:  655 nm 
  • Emission maximum:  670 nm
  • Color:  Far red

Specifications

Unit Size1 mg
ApplicationsImmunofluorescence, Glycobiology
Recommended UsageThe recommended concentration range for use is 5-20 µg/ml. If a precipitate forms upon long-term storage, warm to 37 ºC.
Recommended Storage2-8 °C
Maximum Excitation655 nm
Maximum Emission670 nm
Solution10 mM HEPES, 0.15 M NaCl, pH 7.5, 0.08% sodium azide, 0.1 mM CaCl2.
Concentration1 mg active conjugate/ml
ConjugateDyLight 649
Color of FluorescenceFar Red
Sugar Specificity[GlcNAc]1-3, N-Acetylglucosamine

Documents

Product FAQs

The Lycopersicon esculentum (tomato) lectin has been widely reported as an effective blood vessel marker for in vivo vascular perfusion studies in rodent species. Investigators have primarily utilized one of the fluorophore conjugated tomato lectin formats to trace blood vasculature in normal and diseased animals via tail vein or intracardiac injection. However, the biotinylated format has also been used. It allows for flexibility in subsequent visualization by way of either fluorescence or enzyme-based methods. Published references are best source for procedural details. Examples of references where biotinylated tomato lectins have been applied via in vivo perfusion are featured below: Robertson, R.T., et al. (2014) Histochem. Cell Biol. 143(2) Thurston, G., et al. (1998) Am. J Pathol. 153(4):1099-1112

Citations

Technical Information

Tomato lectin is a very stable single subunit glycoprotein containing about 50 percent arabinose and galactose and may form multimeric aggregates in solution. Tomato lectin, although sharing some specificities with potato lectin, Datura lectin, and wheat germ agglutinin, has been reported to be dissimilar in many respects. LEL binds well to glycophorin and Tamm-Horsfall glycoprotein.

DyLight™ 649 conjugated tomato lectin emits in the far red and is an excellent second label in systems with green/yellow fluorescence such as GFP expressed in transgenic animals, or with fluorescein conjugates in standard double label studies. The tomato lectin complements our existing range of lectin reagents and should be a valuable tool in examining rodent tumor angiogenesis, tracing neovascular development in xenograft models and brain research.

Accompanying each fluorescent lectin is an analysis data sheet summarizing the results of our quality control tests and providing pertinent information on the product. All of these reagents are supplied as solutions preserved with sodium azide.

Inhibiting/Eluting Sugar: Chitin Hydrolysate

The post Lycopersicon Esculentum (Tomato) Lectin (LEL, TL), DyLight® 649 appeared first on VectorLabs.

]]> Lycopersicon Esculentum (Tomato) Lectin (LEL, TL), Texas Red™ https://staging.vectorlabs.com/products/texas-red-lycopersicon-esculentum-lectin/ Tue, 02 May 2023 02:05:53 +0000 https://staging.vectorlabs.com/?post_type=product&p=13891 Description

Tomato lectin, although sharing some specificities with potato lectin, Datura lectin, and wheat germ agglutinin, has been reported to be dissimilar in many respects. LEL binds well to glycophorin and Tamm-Horsfall glycoprotein and has been used effectively to label vascular endothelium in rodents.

Texas Red® labeled Tomato lectin has an appropriate number of fluorochromes bound to provide the optimum staining characteristics for this lectin. This conjugate is supplied essentially free of unconjugated fluorochromes. The excitation maximum is at 595 nm and the emission maximum is at 615 nm.

Specifications

Unit Size 1 mg
Applications Immunofluorescence, Glycobiology
Recommended Usage If a precipitate forms upon long-term storage, warm to 37 ºC.
Recommended Storage 2-8 °C
Maximum Excitation 595-604 nm
Maximum Emission 606-615 nm
Solution 10 mM HEPES, 0.15 M NaCl, pH 7.5, 0.08% sodium azide 0.1 mM CaCl2.
Concentration 1 mg active conjugate/ml
Conjugate Texas Red
Color of Fluorescence Red
Sugar Specificity [GlcNAc]1-3, N-Acetylglucosamine

The post Lycopersicon Esculentum (Tomato) Lectin (LEL, TL), Texas Red™ appeared first on VectorLabs.

]]>

Description

Tomato lectin, although sharing some specificities with potato lectin, Datura lectin, and wheat germ agglutinin, has been reported to be dissimilar in many respects. LEL binds well to glycophorin and Tamm-Horsfall glycoprotein and has been used effectively to label vascular endothelium in rodents.

Texas Red® labeled Tomato lectin has an appropriate number of fluorochromes bound to provide the optimum staining characteristics for this lectin. This conjugate is supplied essentially free of unconjugated fluorochromes. The excitation maximum is at 595 nm and the emission maximum is at 615 nm.

Specifications

Unit Size1 mg
ApplicationsImmunofluorescence, Glycobiology
Recommended UsageIf a precipitate forms upon long-term storage, warm to 37 ºC.
Recommended Storage2-8 °C
Maximum Excitation595-604 nm
Maximum Emission606-615 nm
Solution10 mM HEPES, 0.15 M NaCl, pH 7.5, 0.08% sodium azide 0.1 mM CaCl2.
Concentration1 mg active conjugate/ml
ConjugateTexas Red
Color of FluorescenceRed
Sugar Specificity[GlcNAc]1-3, N-Acetylglucosamine

Documents

Product FAQs

The Lycopersicon esculentum (tomato) lectin has been widely reported as an effective blood vessel marker for in vivo vascular perfusion studies in rodent species. Investigators have primarily utilized one of the fluorophore conjugated tomato lectin formats to trace blood vasculature in normal and diseased animals via tail vein or intracardiac injection. However, the biotinylated format has also been used. It allows for flexibility in subsequent visualization by way of either fluorescence or enzyme-based methods. Published references are best source for procedural details. Examples of references where biotinylated tomato lectins have been applied via in vivo perfusion are featured below: Robertson, R.T., et al. (2014) Histochem. Cell Biol. 143(2) Thurston, G., et al. (1998) Am. J Pathol. 153(4):1099-1112

Citations

Technical Information

Tomato lectin is a very stable single subunit glycoprotein containing about 50 percent arabinose and galactose and may form multimeric aggregates in solution. 

Tomato lectin (from Lycopersicon esculentum) is an effective marker of blood vessels and microglial cells in rodents. Conjugation of the lectin with a fluorophore facilitates fast, one-step detection and visualization using intravascular perfusion methods or direct application to tissue sections.

Texas Red® or DyLight™ 594 conjugated tomato lectin provides an excellent contrast to green/yellow fluorescence such as GFP expressed in transgenic animals, or with fluorescein conjugates in standard double label studies. The tomato lectin complements our existing range of lectin reagents and should be a valuable tool in examining rodent tumor angiogenesis, tracing neovascular development in xenograft models and brain research.

Accompanying each fluorescent lectin is an analysis data sheet summarizing the results of our quality control tests and providing pertinent information on the product. All of these reagents are supplied as solutions preserved with sodium azide.

Inhibiting/Eluting Sugar: Chitin Hydrolysate

The post Lycopersicon Esculentum (Tomato) Lectin (LEL, TL), Texas Red™ appeared first on VectorLabs.

]]> Datura Stramonium Lectin (DSL), Unconjugated https://staging.vectorlabs.com/products/unconj-datura-stramonium-lectin-dsl/ Thu, 27 Apr 2023 14:35:13 +0000 https://staging.vectorlabs.com/?post_type=product&p=12426 Description

The carbohydrate binding site recognizes (β-1,4) linked N-acetylglucosamine oligomers, preferring chitobiose or chitotriose over a single N-acetylglucosamine residue. This lectin binds well in the acidic pH range but its affinity decreases above pH 8.0. DSL also binds well to N-acetyllactosamine and oligomers containing repeating N-acetyllactosamine sequences. A branched pentasaccharide including two N-acetyllactosamine disaccharides linked to mannose (β-1,6) and (β-1,2) was reported to be the most potent inhibitor of agglutination.

Specifications

More Information
Unit Size 5 mg
Applications Glycobiology, Mitogenic Stimulation
Recommended Usage Though many buffers can be employed for reconstituting and diluting this lectin, 10 mM HEPES buffered saline, pH 8.5, 0.1 mM CaCl2 is recommended. For preserving solutions stored at 4 ºC, 0.08% sodium azide can be used. Aggregation may occur with time if stored at concentrations greater than 2 mg/ml.
Recommended Storage 2-8 °C; for long term storage, aliquots may be stored frozen or preserved with 0.08% sodium azide in the recommended buffer and stored at 2-8 °C
Conjugate Unconjugated
Sugar Specificity [GlcNAc]1-3, N-Acetylglucosamine

The post Datura Stramonium Lectin (DSL), Unconjugated appeared first on VectorLabs.

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Description

The carbohydrate binding site recognizes (β-1,4) linked N-acetylglucosamine oligomers, preferring chitobiose or chitotriose over a single N-acetylglucosamine residue. This lectin binds well in the acidic pH range but its affinity decreases above pH 8.0. DSL also binds well to N-acetyllactosamine and oligomers containing repeating N-acetyllactosamine sequences. A branched pentasaccharide including two N-acetyllactosamine disaccharides linked to mannose (β-1,6) and (β-1,2) was reported to be the most potent inhibitor of agglutination.

Specifications

More Information
Unit Size5 mg
ApplicationsGlycobiology, Mitogenic Stimulation
Recommended UsageThough many buffers can be employed for reconstituting and diluting this lectin, 10 mM HEPES buffered saline, pH 8.5, 0.1 mM CaCl2 is recommended. For preserving solutions stored at 4 ºC, 0.08% sodium azide can be used. Aggregation may occur with time if stored at concentrations greater than 2 mg/ml.
Recommended Storage2-8 °C; for long term storage, aliquots may be stored frozen or preserved with 0.08% sodium azide in the recommended buffer and stored at 2-8 °C
ConjugateUnconjugated
Sugar Specificity[GlcNAc]1-3, N-Acetylglucosamine

Documents

Citations

Technical Information

DSL contains two chains of 40 kDa and 46 kDa joined by disulfide bonds. This lectin is free of a reported 32 kDa contaminant protein. 

Inhibiting/Eluting Sugar: Chitin Hydrolysate

The post Datura Stramonium Lectin (DSL), Unconjugated appeared first on VectorLabs.

]]> Lycopersicon esculentum (Tomato) Lectin (LEL, TL), Unconjugated https://staging.vectorlabs.com/products/unconj-lycopersicon-esculentum-tomato-lectin/ Thu, 27 Apr 2023 14:31:30 +0000 https://staging.vectorlabs.com/?post_type=product&p=12418 Description

Tomato lectin (from Lycopersicon esculentum) is an effective marker of blood vessels and microglial cells in rodents. Conjugation of the lectin with a fluorophore facilitates fast, one-step detection and visualization using intravascular perfusion methods or direct application to tissue sections.

Specifications

Unit Size 2 mg
Applications Glycobiology
Recommended Usage Although many buffers can be employed for reconstituting this lectin, 10 mM HEPES buffered saline, pH 8.5, 0.1 mM CaCl2 is recommended.
Recommended Storage 2-8 °C; for long term storage, aliquots may be stored frozen or preserved with 0.08% sodium azide in the recommended buffer and stored at 2-8 °C
Conjugate Unconjugated
Sugar Specificity [GlcNAc]1-3, N-Acetylglucosamine

The post Lycopersicon esculentum (Tomato) Lectin (LEL, TL), Unconjugated appeared first on VectorLabs.

]]>

Description

Tomato lectin (from Lycopersicon esculentum) is an effective marker of blood vessels and microglial cells in rodents. Conjugation of the lectin with a fluorophore facilitates fast, one-step detection and visualization using intravascular perfusion methods or direct application to tissue sections.

Specifications

Unit Size2 mg
ApplicationsGlycobiology
Recommended UsageAlthough many buffers can be employed for reconstituting this lectin, 10 mM HEPES buffered saline, pH 8.5, 0.1 mM CaCl2 is recommended.
Recommended Storage2-8 °C; for long term storage, aliquots may be stored frozen or preserved with 0.08% sodium azide in the recommended buffer and stored at 2-8 °C
ConjugateUnconjugated
Sugar Specificity[GlcNAc]1-3, N-Acetylglucosamine

Documents

Citations

Technical Information

Tomato lectin (from Lycopersicon esculentum) is a very stable single subunit glycoprotein containing about 50 percent arabinose and galactose and may form multimeric aggregates in solution. Tomato lectin, although sharing some specificities with potato lectin, Datura lectin, and wheat germ agglutinin, has been reported to be dissimilar in many respects. LEL binds well to glycophorin and Tamm-Horsfall glycoprotein and has been used effectively to label vascular endothelium in rodents.

Inhibiting/Eluting Sugar: Chitin Hydrolysate

The post Lycopersicon esculentum (Tomato) Lectin (LEL, TL), Unconjugated appeared first on VectorLabs.

]]> Datura Stramonium Lectin (DSL), Fluorescein https://staging.vectorlabs.com/products/fluorescein-datura-stramonium-lectin-dsl/ Tue, 25 Apr 2023 19:00:29 +0000 https://staging.vectorlabs.com/?post_type=product&p=11938 Description

The carbohydrate binding site recognizes (β-1,4) linked N-acetylglucosamine oligomers, preferring chitobiose or chitotriose over a single N-acetylglucosamine residue. This lectin binds well in the acidic pH range but its affinity decreases above pH 8.0.

DSL also binds well to N-acetyllactosamine and oligomers containing repeating N-acetyllactosamine sequences. A branched pentasaccharide including two N-acetyllactosamine disaccharides linked to mannose (β-1,6) and (β-1,2) was reported to be the most potent inhibitor of agglutination.

Fluorescein labeled DSL has an appropriate number of fluorochromes bound to provide the optimum staining characteristics for this lectin. This conjugate is supplied essentially free of unconjugated fluorochromes. The excitation maximum is at 495 nm and the emission maximum is at 515 nm.

Specifications

Unit Size 2 mg
Applications Immunofluorescence, Glycobiology
Recommended Usage The recommended concentration range for use is 5-20 µg/ml.
Recommended Storage 2-8°C
Maximum Excitation 495-500 nm
Maximum Emission 514-521 nm
Solution 10 mM HEPES, 0.15 M NaCl, pH 7.5, 0.08% sodium azide, 0.1 mM CaCl2
Concentration 2 mg active conjugate/ml
Conjugate Fluorescein
Color of Fluorescence Green
Sugar Specificity [GlcNAc]1-3, N-Acetylglucosamine

The post Datura Stramonium Lectin (DSL), Fluorescein appeared first on VectorLabs.

]]>

Description

The carbohydrate binding site recognizes (β-1,4) linked N-acetylglucosamine oligomers, preferring chitobiose or chitotriose over a single N-acetylglucosamine residue. This lectin binds well in the acidic pH range but its affinity decreases above pH 8.0.

DSL also binds well to N-acetyllactosamine and oligomers containing repeating N-acetyllactosamine sequences. A branched pentasaccharide including two N-acetyllactosamine disaccharides linked to mannose (β-1,6) and (β-1,2) was reported to be the most potent inhibitor of agglutination. 

Fluorescein labeled DSL has an appropriate number of fluorochromes bound to provide the optimum staining characteristics for this lectin. This conjugate is supplied essentially free of unconjugated fluorochromes. The excitation maximum is at 495 nm and the emission maximum is at 515 nm.

Specifications

Unit Size2 mg
ApplicationsImmunofluorescence, Glycobiology
Recommended UsageThe recommended concentration range for use is 5-20 µg/ml.
Recommended Storage2-8°C
Maximum Excitation495-500 nm
Maximum Emission514-521 nm
Solution10 mM HEPES, 0.15 M NaCl, pH 7.5, 0.08% sodium azide, 0.1 mM CaCl2
Concentration2 mg active conjugate/ml
ConjugateFluorescein
Color of FluorescenceGreen
Sugar Specificity[GlcNAc]1-3, N-Acetylglucosamine

Documents

Citations

Technical Information

DSL contains two chains of 40 kDa and 46 kDa joined by disulfide bonds. This lectin is free of a reported 32 kDa contaminant protein. 

Accompanying each fluorescent lectin is an analysis data sheet summarizing the results of our quality control tests and providing pertinent information on the product. All of these reagents are supplied as solutions preserved with sodium azide.

Inhibiting/Eluting Sugar: Chitin Hydrolysate

The post Datura Stramonium Lectin (DSL), Fluorescein appeared first on VectorLabs.

]]> Lycopersicon esculentum (Tomato) Lectin (LEL, TL), Fluorescein https://staging.vectorlabs.com/products/fluorescein-lycopersicon-esculentum-tomato/ Tue, 25 Apr 2023 18:57:03 +0000 https://staging.vectorlabs.com/?post_type=product&p=11930 Description

Tomato lectin (from Lycopersicon esculentum) is an effective marker of blood vessels and microglial cells in rodents. Conjugation of the lectin with fluorescein facilitates fast, one-step detection and visualization using intravascular perfusion methods or direct application to tissue sections.

Specifications

Unit Size 1 mg
Applications Immunofluorescence, Glycobiology
Recommended Usage The recommended concentration range for use is 5-20 µg/ml.
Recommended Storage 2-8°C
Maximum Excitation 495-500 nm
Maximum Emission 514-521 nm
Solution 10 mM HEPES, 0.15 M NaCl, pH 7.5, 0.08% sodium azide, 0.1 mM CaCl2, 5mg/ml β cyclodextrin
Concentration 2 mg active conjugate/ml
Conjugate Fluorescein
Color of Fluorescence Green
Sugar Specificity [GlcNAc]1-3, N-Acetylglucosamine

The post Lycopersicon esculentum (Tomato) Lectin (LEL, TL), Fluorescein appeared first on VectorLabs.

]]>

Description

Tomato lectin (from Lycopersicon esculentum) is an effective marker of blood vessels and microglial cells in rodents. Conjugation of the lectin with fluorescein facilitates fast, one-step detection and visualization using intravascular perfusion methods or direct application to tissue sections.

Specifications

Unit Size1 mg
ApplicationsImmunofluorescence, Glycobiology
Recommended UsageThe recommended concentration range for use is 5-20 µg/ml.
Recommended Storage2-8°C
Maximum Excitation495-500 nm
Maximum Emission514-521 nm
Solution10 mM HEPES, 0.15 M NaCl, pH 7.5, 0.08% sodium azide, 0.1 mM CaCl2, 5mg/ml β cyclodextrin
Concentration2 mg active conjugate/ml
ConjugateFluorescein
Color of FluorescenceGreen
Sugar Specificity[GlcNAc]1-3, N-Acetylglucosamine

Documents

Product FAQs

Yes, there are a number of published references describing the use of TrueVIEW Autofluorescence Quenching Kit in the scientific literature. Please refer to a partial list of these publications in the Technical Information section of the product detail page for SP-8400.

Citations

Technical Information

Tomato lectin is a very stable single subunit glycoprotein containing about 50 percent arabinose and galactose and may form multimeric aggregates in solution. Tomato lectin, although sharing some specificities with potato lectin, Datura lectin, and wheat germ agglutinin, has been reported to be dissimilar in many respects. LEL binds well to glycophorin and Tamm-Horsfall glycoprotein and has been used effectively to label vascular endothelium in rodents.

Accompanying each fluorescent lectin is an analysis data sheet summarizing the results of our quality control tests and providing pertinent information on the product. All of these reagents are supplied as solutions preserved with sodium azide.

Fluorescein labeled Tomato lectin has an appropriate number of fluorochromes bound to provide the optimum staining characteristics for this lectin. This conjugate is supplied essentially free of unconjugated fluorochromes.

  • Excitation maximum:  495 nm
  • Emission maximum:  515 nm
  • Color:  Green

Inhibiting/Eluting Sugar: Chitin Hydrolysate

The post Lycopersicon esculentum (Tomato) Lectin (LEL, TL), Fluorescein appeared first on VectorLabs.

]]> Lycopersicon Esculentum (Tomato) Lectin (LEL, TL), DyLight® 594 https://staging.vectorlabs.com/products/dylight-594-lycopersicon-esculentum-tomato/ Tue, 25 Apr 2023 15:37:12 +0000 https://staging.vectorlabs.com/?post_type=product&p=11770 Description

Tomato lectin (from Lycopersicon esculentum) is an effective marker of blood vessels and microglial cells in rodents. Conjugation of the lectin with a fluorophore facilitates fast, one-step detection and visualization using intravascular perfusion methods or direct application to tissue sections.

DyLight™ 594 labeled tomato lectin has an appropriate number of fluorochromes bound to provide the optimum staining characteristics for this lectin. This conjugate is supplied essentially free of unconjugated fluorochromes.

  • Excitation maximum:  592 nm
  • Emission maximum:  617 nm
  • Color:  Red

Specifications

Unit Size 1 mg
Applications Immunofluorescence, Glycobiology
Recommended Usage The recommended concentration range for use is 5-20 µg/ml. If a precipitate forms upon long-term storage, warm to 37 ºC.
Recommended Storage 2-8 °C
Maximum Excitation 592 nm
Maximum Emission 617 nm
Solution 10 mM HEPES, 0.15 M NaCl, pH 7.5, 0.08% sodium azide, 0.1 mM CaCl2.
Concentration 1 mg active conjugate/ml
Conjugate DyLight 594
Color of Fluorescence Red
Sugar Specificity [GlcNAc]1-3, N-Acetylglucosamine

The post Lycopersicon Esculentum (Tomato) Lectin (LEL, TL), DyLight® 594 appeared first on VectorLabs.

]]>

Description

Tomato lectin (from Lycopersicon esculentum) is an effective marker of blood vessels and microglial cells in rodents. Conjugation of the lectin with a fluorophore facilitates fast, one-step detection and visualization using intravascular perfusion methods or direct application to tissue sections.

DyLight™ 594 labeled tomato lectin has an appropriate number of fluorochromes bound to provide the optimum staining characteristics for this lectin. This conjugate is supplied essentially free of unconjugated fluorochromes.

  • Excitation maximum:  592 nm
  • Emission maximum:  617 nm
  • Color:  Red

Specifications

Unit Size1 mg
ApplicationsImmunofluorescence, Glycobiology
Recommended UsageThe recommended concentration range for use is 5-20 µg/ml. If a precipitate forms upon long-term storage, warm to 37 ºC.
Recommended Storage2-8 °C
Maximum Excitation592 nm
Maximum Emission617 nm
Solution10 mM HEPES, 0.15 M NaCl, pH 7.5, 0.08% sodium azide, 0.1 mM CaCl2.
Concentration1 mg active conjugate/ml
ConjugateDyLight 594
Color of FluorescenceRed
Sugar Specificity[GlcNAc]1-3, N-Acetylglucosamine

Documents

Product FAQs

Yes, there are a number of published references describing the use of TrueVIEW Autofluorescence Quenching Kit in the scientific literature. Please refer to a partial list of these publications in the Technical Information section of the product detail page for SP-8400.

Citations

Technical Information

Tomato lectin is a very stable single subunit glycoprotein containing about 50 percent arabinose and galactose and may form multimeric aggregates in solution. Tomato lectin, although sharing some specificities with potato lectin, Datura lectin, and wheat germ agglutinin, has been reported to be dissimilar in many respects. LEL binds well to glycophorin and Tamm-Horsfall glycoprotein and has been used effectively to label vascular endothelium in rodents.

Texas Red® or DyLight® 594 conjugated tomato lectin provides an excellent contrast to green/yellow fluorescence such as GFP expressed in transgenic animals, or with fluorescein conjugates in standard double label studies. The tomato lectin complements our existing range of lectin reagents and should be a valuable tool in examining rodent tumor angiogenesis, tracing neovascular development in xenograft models and brain research.

Accompanying each fluorescent lectin is an analysis data sheet summarizing the results of our quality control tests and providing pertinent information on the product. All of these reagents are supplied as solutions preserved with sodium azide.

Inhibiting/Eluting Sugar: Chitin Hydrolysate

The post Lycopersicon Esculentum (Tomato) Lectin (LEL, TL), DyLight® 594 appeared first on VectorLabs.

]]> Lycopersicon Esculentum (Tomato) Lectin (LEL, TL), DyLight® 488 https://staging.vectorlabs.com/products/dylight-488-lycopersicon-esculentum-tomato/ Tue, 25 Apr 2023 15:30:52 +0000 https://staging.vectorlabs.com/?post_type=product&p=11761 Description

Tomato lectin (from Lycopersicon esculentum) is an effective marker of blood vessels and microglial cells in rodents. Conjugation of the lectin with a fluorophore facilitates fast, one-step detection and visualization using intravascular perfusion methods or direct application to tissue sections.

DyLight™ 488 labeled tomato lectin has an appropriate number of fluorochromes bound to provide the optimum staining characteristics for this lectin. This conjugate is supplied essentially free of unconjugated fluorochromes. The excitation maximum is at 493 nm and the emission maximum is at 518 nm.

Specifications

Unit Size 1 mg
Applications Immunofluorescence, Glycobiology
Recommended Usage The recommended concentration range for use is 5-20 µg/ml. If a precipitate forms upon long-term storage, warm to 37 ºC.
Recommended Storage 2-8 °C
Maximum Excitation 493 nm
Maximum Emission 518 nm
Solution 10 mM HEPES, 0.15 M NaCl, pH 7.5, 0.08% sodium azide, 0.1 mM CaCl2.
Concentration 1 mg active conjugate/ml
Conjugate DyLight 488
Color of Fluorescence Green
Sugar Specificity [GlcNAc]1-3, N-Acetylglucosamine

The post Lycopersicon Esculentum (Tomato) Lectin (LEL, TL), DyLight® 488 appeared first on VectorLabs.

]]>

Description

Tomato lectin (from Lycopersicon esculentum) is an effective marker of blood vessels and microglial cells in rodents. Conjugation of the lectin with a fluorophore facilitates fast, one-step detection and visualization using intravascular perfusion methods or direct application to tissue sections.

DyLight™ 488 labeled tomato lectin has an appropriate number of fluorochromes bound to provide the optimum staining characteristics for this lectin. This conjugate is supplied essentially free of unconjugated fluorochromes. The excitation maximum is at 493 nm and the emission maximum is at 518 nm.

Specifications

Unit Size1 mg
ApplicationsImmunofluorescence, Glycobiology
Recommended UsageThe recommended concentration range for use is 5-20 µg/ml. If a precipitate forms upon long-term storage, warm to 37 ºC.
Recommended Storage2-8 °C
Maximum Excitation493 nm
Maximum Emission518 nm
Solution10 mM HEPES, 0.15 M NaCl, pH 7.5, 0.08% sodium azide, 0.1 mM CaCl2.
Concentration1 mg active conjugate/ml
ConjugateDyLight 488
Color of FluorescenceGreen
Sugar Specificity[GlcNAc]1-3, N-Acetylglucosamine

Documents

Product FAQs

Yes, there are a number of published references describing the use of TrueVIEW Autofluorescence Quenching Kit in the scientific literature. Please refer to a partial list of these publications in the Technical Information section of the product detail page for SP-8400.

Citations

Technical Information

Tomato lectin is a very stable single subunit glycoprotein containing about 50 percent arabinose and galactose and may form multimeric aggregates in solution. Tomato lectin, although sharing some specificities with potato lectin, Datura lectin, and wheat germ agglutinin, has been reported to be dissimilar in many respects. LEL binds well to glycophorin and Tamm-Horsfall glycoprotein and has been used effectively to label vascular endothelium in rodents.

Accompanying each fluorescent lectin is an analysis data sheet summarizing the results of our quality control tests and providing pertinent information on the product. All of these reagents are supplied as solutions preserved with sodium azide.

Inhibiting/Eluting Sugar: Chitin Hydrolysate

The post Lycopersicon Esculentum (Tomato) Lectin (LEL, TL), DyLight® 488 appeared first on VectorLabs.

]]> Datura Stramonium Lectin (DSL), Biotinylated https://staging.vectorlabs.com/products/biotinylated-datura-stramonium-lectin-dsl/ Wed, 19 Apr 2023 21:30:25 +0000 https://staging.vectorlabs.com/?post_type=product&p=11025 Description

The carbohydrate binding site recognizes (β-1,4) linked N-acetylglucosamine oligomers, preferring chitobiose or chitotriose over a single N-acetylglucosamine residue. This lectin binds well in the acidic pH range but its affinity decreases above pH 8.0.

DSL also binds well to N-acetyllactosamine and oligomers containing repeating N-acetyllactosamine sequences. A branched pentasaccharide including two N-acetyllactosamine disaccharides linked to mannose (β-1,6) and (β-1,2) was reported to be the most potent inhibitor of agglutination.

Biotinylated Datura stramonium lectin has an appropriate number of biotins bound to provide the optimum staining characteristics for this lectin. This conjugate is supplied essentially free of unconjugated biotins and is preserved with sodium azide.

Specifications

Unit Size 2 mg
Applications Immunohistochemistry / Immunocytochemistry, Immunofluorescence, Blotting Applications, Elispot, ELISAs, Glycobiology
Recommended Usage For most applications, we recommend a freshly prepared working solution of 5-20 µg/ml in the below buffer.
Recommended Storage 2-8 °C; Store frozen for long term storage
Solution 10 mM HEPES, pH 7.5, 0.15 M NaCl, 0.08% sodium azide, 0.1 mM CaCl2.
Concentration 2 mg active conjugate/ml
Conjugate Biotinylated
Sugar Specificity [GlcNAc]1-3, N-Acetylglucosamine

Technical Information

DSL contains two chains of 40 kDa and 46 kDa joined by disulfide bonds. This lectin is free of a reported 32 kDa contaminant protein.

This biotinylated lectin is an ideal intermediate for examining glycoconjugates using the Biotin-Avidin/Streptavidin System. First the biotinylated lectin is added, followed by the VECTASTAIN ABC Reagent, Avidin D conjugate, or streptavidin derivative.

Inhibiting/Eluting Sugar: Chitin Hydrolysate

The post Datura Stramonium Lectin (DSL), Biotinylated appeared first on VectorLabs.

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Description

The carbohydrate binding site recognizes (β-1,4) linked N-acetylglucosamine oligomers, preferring chitobiose or chitotriose over a single N-acetylglucosamine residue. This lectin binds well in the acidic pH range but its affinity decreases above pH 8.0.

DSL also binds well to N-acetyllactosamine and oligomers containing repeating N-acetyllactosamine sequences. A branched pentasaccharide including two N-acetyllactosamine disaccharides linked to mannose (β-1,6) and (β-1,2) was reported to be the most potent inhibitor of agglutination. 

Biotinylated Datura stramonium lectin has an appropriate number of biotins bound to provide the optimum staining characteristics for this lectin. This conjugate is supplied essentially free of unconjugated biotins and is preserved with sodium azide.

Specifications

Unit Size2 mg
ApplicationsImmunohistochemistry / Immunocytochemistry, Immunofluorescence, Blotting Applications, Elispot, ELISAs, Glycobiology
Recommended UsageFor most applications, we recommend a freshly prepared working solution of 5-20 µg/ml in the below buffer.
Recommended Storage2-8 °C; Store frozen for long term storage
Solution10 mM HEPES, pH 7.5, 0.15 M NaCl, 0.08% sodium azide, 0.1 mM CaCl2.
Concentration2 mg active conjugate/ml
ConjugateBiotinylated
Sugar Specificity[GlcNAc]1-3, N-Acetylglucosamine

Documents

Product FAQs

From our experience we have found that some lectins require Ca++ to be present for optimal binding activity. We suggest using calcium chloride (CaCl2) to fortify working solutions and ensure a minimum level of Ca++ is met. This may be particularly pertinent if using phosphate based buffers as diluents and storage solutions.

Citations

Technical Information

DSL contains two chains of 40 kDa and 46 kDa joined by disulfide bonds. This lectin is free of a reported 32 kDa contaminant protein. 

This biotinylated lectin is an ideal intermediate for examining glycoconjugates using the Biotin-Avidin/Streptavidin System. First the biotinylated lectin is added, followed by the VECTASTAIN ABC Reagent, Avidin D conjugate, or streptavidin derivative.

Inhibiting/Eluting Sugar: Chitin Hydrolysate

The post Datura Stramonium Lectin (DSL), Biotinylated appeared first on VectorLabs.

]]> Lycopersicon esculentum (Tomato) Lectin (LEL, TL), Biotinylated https://staging.vectorlabs.com/products/biotinylated-lycopersicon-tomato-lectin/ Wed, 19 Apr 2023 21:24:05 +0000 https://staging.vectorlabs.com/?post_type=product&p=11015 Description

Tomato lectin, although sharing some specificities with potato lectin, Datura lectin, and wheat germ agglutinin, has been reported to be dissimilar in many respects. LEL binds well to glycophorin and Tamm-Horsfall glycoprotein and has been used effectively to label vascular endothelium in rodents.

Biotinylated tomato lectin has an appropriate number of biotins bound to provide the optimum staining characteristics for this lectin. This conjugate is supplied essentially free of unconjugated biotins and is preserved with sodium azide.

Specifications

Unit Size 1 mg
Applications Immunohistochemistry / Immunocytochemistry, Immunofluorescence, Blotting Applications, Elispot, ELISAs, Glycobiology
Recommended Usage For most applications, we recommend a freshly prepared working solution of 5-20 µg/ml in the below buffer.
Recommended Storage 2-8 °C; Store frozen for long term storage
Solution 10 mM HEPES, pH 7.5, 0.15 M NaCl, 0.08% sodium azide, 0.1 mM CaCl2
Concentration 2 mg active conjugate/ml
Conjugate Biotinylated
Sugar Specificity [GlcNAc]1-3, N-Acetylglucosamine

Technical Information

Tomato lectin is a very stable single subunit glycoprotein containing about 50 percent arabinose and galactose and may form multimeric aggregates in solution.

This biotinylated lectin is an ideal intermediate for examining glycoconjugates using the Biotin-Avidin/Streptavidin System. First the biotinylated lectin is added, followed by the VECTASTAIN® ABC Reagent, Avidin D conjugate, or streptavidin derivative.

Inhibiting/Eluting Sugar: Chitin Hydrolysate

The post Lycopersicon esculentum (Tomato) Lectin (LEL, TL), Biotinylated appeared first on VectorLabs.

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Description

Tomato lectin, although sharing some specificities with potato lectin, Datura lectin, and wheat germ agglutinin, has been reported to be dissimilar in many respects. LEL binds well to glycophorin and Tamm-Horsfall glycoprotein and has been used effectively to label vascular endothelium in rodents.

Biotinylated tomato lectin has an appropriate number of biotins bound to provide the optimum staining characteristics for this lectin. This conjugate is supplied essentially free of unconjugated biotins and is preserved with sodium azide.

Specifications

Unit Size1 mg
ApplicationsImmunohistochemistry / Immunocytochemistry, Immunofluorescence, Blotting Applications, Elispot, ELISAs, Glycobiology
Recommended UsageFor most applications, we recommend a freshly prepared working solution of 5-20 µg/ml in the below buffer.
Recommended Storage2-8 °C; Store frozen for long term storage
Solution10 mM HEPES, pH 7.5, 0.15 M NaCl, 0.08% sodium azide, 0.1 mM CaCl2
Concentration2 mg active conjugate/ml
ConjugateBiotinylated
Sugar Specificity[GlcNAc]1-3, N-Acetylglucosamine

Documents

Product FAQs

The Lycopersicon esculentum (tomato) lectin has been widely reported as an effective blood vessel marker for in vivo vascular perfusion studies in rodent species. Investigators have primarily utilized one of the fluorophore conjugated tomato lectin formats to trace blood vasculature in normal and diseased animals via tail vein or intracardiac injection. However, the biotinylated format has also been used. It allows for flexibility in subsequent visualization by way of either fluorescence or enzyme-based methods. Published references are best source for procedural details. Examples of references where biotinylated tomato lectins have been applied via in vivo perfusion are featured below: Robertson, R.T., et al. (2014) Histochem. Cell Biol. 143(2) Thurston, G., et al. (1998) Am. J Pathol. 153(4):1099-1112

From our experience we have found that some lectins require Ca++ to be present for optimal binding activity. We suggest using calcium chloride (CaCl2) to fortify working solutions and ensure a minimum level of Ca++ is met. This may be particularly pertinent if using phosphate based buffers as diluents and storage solutions.

Citations

Technical Information

Tomato lectin is a very stable single subunit glycoprotein containing about 50 percent arabinose and galactose and may form multimeric aggregates in solution. 

This biotinylated lectin is an ideal intermediate for examining glycoconjugates using the Biotin-Avidin/Streptavidin System. First the biotinylated lectin is added, followed by the VECTASTAIN® ABC Reagent, Avidin D conjugate, or streptavidin derivative.

Inhibiting/Eluting Sugar: Chitin Hydrolysate

The post Lycopersicon esculentum (Tomato) Lectin (LEL, TL), Biotinylated appeared first on VectorLabs.

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