FP-1064 – VectorLabs https://staging.vectorlabs.com From linker design and synthesis, bioconjugated design and manufacturing, biomolecule labeling and functionalization, and detection system and solutions, our team is here to help you move forward, with impact. Wed, 22 Oct 2025 17:07:12 +0000 en-US hourly 1 https://wordpress.org/?v=6.9 https://staging.vectorlabs.com/wp-content/uploads/2024/11/V-favicon-02-100x100.png FP-1064 – VectorLabs https://staging.vectorlabs.com 32 32 AZDye 405 Azide https://staging.vectorlabs.com/products/azdye-405-azide/ Tue, 19 Sep 2023 17:48:30 +0000 https://staging.vectorlabs.com/?post_type=product&p=21325 Description

AZDye™ 405 Azide can be reacted with terminal alkynes via a copper-catalyzed click reaction (CuAAC). It also reacts with strained cyclooctyne via a copper-free “click chemistry” reaction to form a stable triazole and does not require Cu-catalyst or elevated temperatures.

AZDye™ 405 is a bright, blue-fluorescent probe optimally excited by the 407 nm spectral line of the krypton laser or the 408 nm violet laser diode. This probe is water-soluble and its fluorescence is pH independent over a wide pH range. The brightness and photostability of this dye are best suited to direct imaging of moderate-abundance targets.

AZDye™ 405 is structurally similar to Alexa Fluor® 405 or Cascade Blue, and spectrally is a match to DyLight® 405, Alexa Fluor® 405, CF® 405S Dye, Cascade Blue, or any other sulfonated pyrene based fluorescent dyes.

[caption id="attachment_25931" align="alignnone" width="1000"] Abs/Em Spectra[/caption]

Specifications

Unit Size 1 mg, 5 mg, 25 mg
Abs/Em Maxima 402/424 nm
Extinction Coefficient
35,000
Flow Cytometry Laser Line
405 nm
Microscopy Laser Line
405 nm
Spectrally Similar Dyes Alexa Fluor® 405, CF® 405, Cascade Blue®, DyLight® 405
Molecular weight 640.65 (protonated) 706.60 (sodium salt)
CAS N/A
Solubility Water, DMSO, DMF
Purity >95% (HPLC)
Appearance Yellow solid
Storage Conditions -20°C. Desiccate
Shipping Conditions Ambient temperature

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AZDye™ 405 Azide is a water-soluble, pH-insensitive from pH 4 to pH 10, blue-fluorescent probe with excitation ideally suited for the 407 nm spectral line of the krypton laser or the 408 nm violet laser diode. AZDye™ 405 is often used in multicolor applications, including flow cytometry and super-resolution microscopy using STORM.

Description

AZDye™ 405 Azide can be reacted with terminal alkynes via a copper-catalyzed click reaction (CuAAC). It also reacts with strained cyclooctyne via a copper-free “click chemistry” reaction to form a stable triazole and does not require Cu-catalyst or elevated temperatures.

AZDye™ 405 is a bright, blue-fluorescent probe optimally excited by the 407 nm spectral line of the krypton laser or the 408 nm violet laser diode. This probe is water-soluble and its fluorescence is pH independent over a wide pH range. The brightness and photostability of this dye are best suited to direct imaging of moderate-abundance targets.

AZDye™ 405 is structurally similar to Alexa Fluor® 405 or Cascade Blue, and spectrally is a match to DyLight® 405, Alexa Fluor® 405, CF® 405S Dye, Cascade Blue, or any other sulfonated pyrene based fluorescent dyes.

Specifications

Unit Size1 mg, 5 mg, 25 mg
Abs/Em Maxima402/424 nm
Extinction Coefficient
35,000
Flow Cytometry Laser Line
405 nm
Microscopy Laser Line
405 nm
Spectrally Similar DyesAlexa Fluor® 405, CF® 405, Cascade Blue®, DyLight® 405
Molecular weight640.65 (protonated) 706.60 (sodium salt)
CASN/A
SolubilityWater, DMSO, DMF
Purity>95% (HPLC)
AppearanceYellow solid
Storage Conditions-20°C. Desiccate
Shipping ConditionsAmbient temperature

Abs/Em Spectra

AF405

Documents

Selected References

  1. Schuler, D., et al. (2021). Differential Labeling of Chemically Modified Peptides and Lipids among Cyanobacteria Planktothrix and Microcystis. Microorganisms, 9, 1578. [MDPI]

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