Fluorescein – VectorLabs https://staging.vectorlabs.com From linker design and synthesis, bioconjugated design and manufacturing, biomolecule labeling and functionalization, and detection system and solutions, our team is here to help you move forward, with impact. Wed, 22 Oct 2025 17:11:33 +0000 en-US hourly 1 https://wordpress.org/?v=6.9 https://staging.vectorlabs.com/wp-content/uploads/2024/11/V-favicon-02-100x100.png Fluorescein – VectorLabs https://staging.vectorlabs.com 32 32 Griffonia (Bandeiraea) Simplicifolia Lectin I (GSL I, BSL I), Fluorescein https://staging.vectorlabs.com/products/fluorescein-griffonia-bandeiraea-simplicifolia/ Tue, 02 May 2023 14:14:34 +0000 https://staging.vectorlabs.com/?post_type=product&p=14072 Description

GSL I is a family of glycoproteins with molecular weights of approximately 114 kDa. There are two types of subunits, termed A and B, with slightly different molecular weights. These subunits combine to form tetrameric structures, resulting in five isolectins. The A-rich lectin preferentially agglutinates blood group A erythrocytes and thus appears to be specific for α-N-acetylgalactosamine residues, while the B-rich lectin preferentially agglutinates blood group B cells and is specific for α-galactose residues. Our GSL I is a mixture of the five isolectins. GSL I has been reported to bind several glycoproteins including laminin.

Fluorescein labeled GSL I has an appropriate number of fluorochromes bound to provide the optimum staining characteristics for this lectin. This conjugate is supplied essentially free of unconjugated fluorochromes. The excitation maximum is at 495 nm and the emission maximum is at 515 nm.

Specifications

Unit Size 2 mg, 5 mg
Applications Immunofluorescence, Glycobiology
Recommended Usage The recommended concentration range for use is 5-20 µg/ml.
Recommended Storage 2-8 °C
Maximum Excitation 495-500 nm
Inhibiting and/or Eluting Sugar Mixture of 200 mM galactose (S-9003)/200 mM N-acetylgalactosamine (S-9001).
Maximum Emission 514-521 nm
Solution 10 mM HEPES, 0.15 M NaCl, pH 7.5, 0.08% sodium azide, 0.1 mM CaCl2
Concentration FL-1101-2 (2 mg active conjugate/ml)
FL-1101-5 (5 mg active conjugate/ml)
Conjugate Fluorescein
Color of Fluorescence Green
Sugar Specificity Galactose, N-Acetylgalactosamine

The post Griffonia (Bandeiraea) Simplicifolia Lectin I (GSL I, BSL I), Fluorescein appeared first on VectorLabs.

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Description

GSL I is a family of glycoproteins with molecular weights of approximately 114 kDa. There are two types of subunits, termed A and B, with slightly different molecular weights. These subunits combine to form tetrameric structures, resulting in five isolectins. The A-rich lectin preferentially agglutinates blood group A erythrocytes and thus appears to be specific for α-N-acetylgalactosamine residues, while the B-rich lectin preferentially agglutinates blood group B cells and is specific for α-galactose residues. Our GSL I is a mixture of the five isolectins. GSL I has been reported to bind several glycoproteins including laminin.

Fluorescein labeled GSL I has an appropriate number of fluorochromes bound to provide the optimum staining characteristics for this lectin. This conjugate is supplied essentially free of unconjugated fluorochromes. The excitation maximum is at 495 nm and the emission maximum is at 515 nm.

Specifications

Unit Size2 mg, 5 mg
ApplicationsImmunofluorescence, Glycobiology
Recommended UsageThe recommended concentration range for use is 5-20 µg/ml.
Recommended Storage2-8 °C
Maximum Excitation495-500 nm
Inhibiting and/or Eluting SugarMixture of 200 mM galactose (S-9003)/200 mM N-acetylgalactosamine (S-9001).
Maximum Emission514-521 nm
Solution10 mM HEPES, 0.15 M NaCl, pH 7.5, 0.08% sodium azide, 0.1 mM CaCl2
ConcentrationFL-1101-2 (2 mg active conjugate/ml)
FL-1101-5 (5 mg active conjugate/ml)
ConjugateFluorescein
Color of FluorescenceGreen
Sugar SpecificityGalactose, N-Acetylgalactosamine

Documents

Citations

Technical Information

Accompanying each fluorescent lectin is an analysis data sheet summarizing the results of our quality control tests and providing pertinent information on the product. All of these reagents are supplied as solutions preserved with sodium azide.

Inhibiting/Eluting Sugar: mixture of 200 mM galactose/200 mM N-acetylgalactosamine

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]]> Peanut Agglutinin (PNA), Fluorescein https://staging.vectorlabs.com/products/fluorescein-peanut-agglutinin-pna/ Tue, 02 May 2023 14:09:57 +0000 https://staging.vectorlabs.com/?post_type=product&p=14062 Description

Peanut agglutinin binds preferentially to the T-antigen, a galactosyl (β-1,3) N-acetylgalactosamine structure present in many glycoconjugates such as M and N blood groups, gangliosides, and many other soluble and membrane-associated glycoproteins and glycolipids. With certain exceptions, the receptor sequence for PNA is normally sialylated which prevents the lectin from binding to its receptor oligosaccharide (see Jacalin). Even sialic acid which is not bound directly to the receptor sugars may inhibit binding. The presence of calcium ions in diluents can enhance the binding of PNA to receptors, possibly by neutralizing the negative charges on sialic acid residues adjacent to the receptor sequence.

Fluorescein labeled Peanut agglutinin has an appropriate number of fluorochromes bound to provide the optimum staining characteristics for this lectin. This conjugate is supplied essentially free of unconjugated fluorochromes.

  • Excitation maximum: 495 nm
  • Emission maximum: 515 nm
  • Color: Green

Specifications

Unit Size 5 mg
Applications Immunofluorescence, Glycobiology
Recommended Usage The recommended concentration range for use is 5-20 µg/ml.
Recommended Storage 2-8 °C
Maximum Excitation 495-500 nm
Inhibiting and/or Eluting Sugar 200 mM galactose (S-9003)
Maximum Emission 514-521 nm
Solution 10 mM HEPES, 0.15 M NaCl, pH 7.5, 0.08% sodium azide, 0.1 mM CaCl2, 0.01mM MnCl2
Concentration 5 mg active conjugate/ml
Conjugate Fluorescein
Color of Fluorescence Green
Sugar Specificity Galactose

The post Peanut Agglutinin (PNA), Fluorescein appeared first on VectorLabs.

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Description

Peanut agglutinin binds preferentially to the T-antigen, a galactosyl (β-1,3) N-acetylgalactosamine structure present in many glycoconjugates such as M and N blood groups, gangliosides, and many other soluble and membrane-associated glycoproteins and glycolipids. With certain exceptions, the receptor sequence for PNA is normally sialylated which prevents the lectin from binding to its receptor oligosaccharide (see Jacalin). Even sialic acid which is not bound directly to the receptor sugars may inhibit binding. The presence of calcium ions in diluents can enhance the binding of PNA to receptors, possibly by neutralizing the negative charges on sialic acid residues adjacent to the receptor sequence.

Fluorescein labeled Peanut agglutinin has an appropriate number of fluorochromes bound to provide the optimum staining characteristics for this lectin. This conjugate is supplied essentially free of unconjugated fluorochromes.

  • Excitation maximum: 495 nm
  • Emission maximum: 515 nm
  • Color: Green

Specifications

Unit Size5 mg
ApplicationsImmunofluorescence, Glycobiology
Recommended UsageThe recommended concentration range for use is 5-20 µg/ml.
Recommended Storage2-8 °C
Maximum Excitation495-500 nm
Inhibiting and/or Eluting Sugar200 mM galactose (S-9003)
Maximum Emission514-521 nm
Solution10 mM HEPES, 0.15 M NaCl, pH 7.5, 0.08% sodium azide, 0.1 mM CaCl2, 0.01mM MnCl2
Concentration5 mg active conjugate/ml
ConjugateFluorescein
Color of FluorescenceGreen
Sugar SpecificityGalactose

Documents

Citations

Technical Information

PNA is useful in distinguishing between normal and tumor tissues and in assessing malignancy in transitional mucosa. In addition, PNA binding can be used to measure cellular maturity in lymphoid tissues, to distinguish a variety of lymphocyte subpopulations in man and experimental animals, and to measure the levels of lymphoid cell populations in many diseases. PNA can be employed in the fractionation of stem cells in mice for use in bone marrow transplantation across histocompatibility barriers.

A major cell surface receptor for PNA may be asialo GM1 ganglioside. Since PNA shares specificity with the antibody to this glycolipid, PNA and the antibody can be used interchangeably in some applications.

Accompanying each fluorescent lectin is an analysis data sheet summarizing the results of our quality control tests and providing pertinent information on the product. All of these reagents are supplied as solutions preserved with sodium azide.

Inhibiting/Eluting Sugar: 200 mM galactose

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]]> Ulex Europaeus Agglutinin I (UEA I), Fluorescein https://staging.vectorlabs.com/products/fluorescein-ulex-europaeus-agglutinin/ Tue, 02 May 2023 05:02:44 +0000 https://staging.vectorlabs.com/?post_type=product&p=14052 Description

Ulex europaeus agglutinin I binds to many glycoproteins and glycolipids containing α-linked fucose residues, such as ABO blood group glycoconjugates. This lectin preferentially binds blood group O cells and has been used to determine secretor status. It has been established as an excellent marker for human endothelial cells.

Fluorescein labeled Ulex europaeus agglutinin I has an appropriate number of fluorochromes bound to provide the optimum staining characteristics for this lectin. This conjugate is supplied essentially free of unconjugated fluorochromes.

  • Excitation maximum: 495 nm
  • Emission maximum: 515 nm
  • Color: Green

Specifications

Unit Size 2 mg, 5 mg
Applications Immunofluorescence, Glycobiology
Recommended Usage The recommended concentration range for use is 5-20 µg/ml.
Recommended Storage 2-8 °C
Maximum Excitation 495-500 nm
Maximum Emission 514-521 nm
Solution 10 mM HEPES, 0.15 M NaCl, pH 7.5, 0.08% sodium azide, 0.1 mM CaCl2
Concentration FL-1061-2 (2 mg active conjugate/ml)
FL-1061-5 (5 mg active conjugate/ml)
Conjugate Fluorescein
Color of Fluorescence Green
Sugar Specificity Fucose, Arabinose

The post Ulex Europaeus Agglutinin I (UEA I), Fluorescein appeared first on VectorLabs.

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Description

Ulex europaeus agglutinin I binds to many glycoproteins and glycolipids containing α-linked fucose residues, such as ABO blood group glycoconjugates. This lectin preferentially binds blood group O cells and has been used to determine secretor status. It has been established as an excellent marker for human endothelial cells.

Fluorescein labeled Ulex europaeus agglutinin I has an appropriate number of fluorochromes bound to provide the optimum staining characteristics for this lectin. This conjugate is supplied essentially free of unconjugated fluorochromes.

  • Excitation maximum: 495 nm
  • Emission maximum: 515 nm
  • Color: Green

Specifications

Unit Size2 mg, 5 mg
ApplicationsImmunofluorescence, Glycobiology
Recommended UsageThe recommended concentration range for use is 5-20 µg/ml.
Recommended Storage2-8 °C
Maximum Excitation495-500 nm
Maximum Emission514-521 nm
Solution10 mM HEPES, 0.15 M NaCl, pH 7.5, 0.08% sodium azide, 0.1 mM CaCl2
ConcentrationFL-1061-2 (2 mg active conjugate/ml)
FL-1061-5 (5 mg active conjugate/ml)
ConjugateFluorescein
Color of FluorescenceGreen
Sugar SpecificityFucose, Arabinose

Documents

Citations

Technical Information

Accompanying each fluorescent lectin is an analysis data sheet summarizing the results of our quality control tests and providing pertinent information on the product. All of these reagents are supplied as solutions preserved with sodium azide.

Inhibiting/Eluting Sugar: 50 mM – 100 mM L-fucose

The post Ulex Europaeus Agglutinin I (UEA I), Fluorescein appeared first on VectorLabs.

]]> Dolichos Biflorus Agglutinin (DBA), Fluorescein https://staging.vectorlabs.com/products/fluorescein-dolichos-biflorus-agglutinin/ Tue, 02 May 2023 04:58:16 +0000 https://staging.vectorlabs.com/?post_type=product&p=14042 Description

Dolichos biflorus agglutinin has a carbohydrate specificity toward α-linked N-acetylgalactosamine.  It has been used to establish secretor status in blood group A individuals by hemagglutination inhibition techniques and for blood typing. This lectin has also been used as a general marker of developing renal collecting ducts.

Fluorescein labeled Dolichos biflorus agglutinin has an appropriate number of fluorochromes bound to provide the optimum staining characteristics for this lectin. This conjugate is supplied essentially free of unconjugated fluorochromes. The excitation maximum is at 495 nm and the emission maximum is at 515 nm.

Specifications

Unit Size 2 mg, 5 mg
Applications Immunofluorescence, Glycobiology
Recommended Usage The recommended concentration range for use is 5-20 µg/ml.
Recommended Storage 2-8 °C
Maximum Excitation 495-500 nm
Maximum Emission 514-521 nm
Solution 10 mM HEPES, 0.15 M NaCl, pH 7.5, 0.08% sodium azide, 0.1 mM CaCl2
Concentration FL-1031-2 (2 mg active conjugate/ml)
FL-1031-5 (5 mg active conjugate/ml)
Conjugate Fluorescein
Color of Fluorescence Green
Sugar Specificity N-Acetylgalactosamine

The post Dolichos Biflorus Agglutinin (DBA), Fluorescein appeared first on VectorLabs.

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Description

Dolichos biflorus agglutinin has a carbohydrate specificity toward α-linked N-acetylgalactosamine.  It has been used to establish secretor status in blood group A individuals by hemagglutination inhibition techniques and for blood typing. This lectin has also been used as a general marker of developing renal collecting ducts.

Fluorescein labeled Dolichos biflorus agglutinin has an appropriate number of fluorochromes bound to provide the optimum staining characteristics for this lectin. This conjugate is supplied essentially free of unconjugated fluorochromes. The excitation maximum is at 495 nm and the emission maximum is at 515 nm.

Specifications

Unit Size2 mg, 5 mg
ApplicationsImmunofluorescence, Glycobiology
Recommended UsageThe recommended concentration range for use is 5-20 µg/ml.
Recommended Storage2-8 °C
Maximum Excitation495-500 nm
Maximum Emission514-521 nm
Solution10 mM HEPES, 0.15 M NaCl, pH 7.5, 0.08% sodium azide, 0.1 mM CaCl2
ConcentrationFL-1031-2 (2 mg active conjugate/ml)
FL-1031-5 (5 mg active conjugate/ml)
ConjugateFluorescein
Color of FluorescenceGreen
Sugar SpecificityN-Acetylgalactosamine

Documents

Citations

Technical Information

Dolichos biflorus agglutinin is a glycoprotein with a molecular weight of about 111 kDa and consists of 4 subunits of approximately equal size. 

Accompanying each fluorescent lectin is an analysis data sheet summarizing the results of our quality control tests and providing pertinent information on the product. All of these reagents are supplied as solutions preserved with sodium azide.

Inhibiting/Eluting Sugar: 200 mM N-acetylgalactosamine

The post Dolichos Biflorus Agglutinin (DBA), Fluorescein appeared first on VectorLabs.

]]> Wheat Germ Agglutinin (WGA), Fluorescein https://staging.vectorlabs.com/products/fluorescein-wheat-germ-agglutinin-wga/ Tue, 02 May 2023 04:52:08 +0000 https://staging.vectorlabs.com/?post_type=product&p=14028 Description

The receptor sugar for WGA is N-acetylglucosamine, with preferential binding to dimers and trimers of this sugar. WGA can bind oligosaccharides containing terminal N-acetylglucosamine or chitobiose, structures which are common to many serum and membrane glycoproteins. Bacterial cell wall peptidoglycans, chitin, cartilage glycosaminoglycans, and glycolipids can also bind WGA. Native WGA has also been reported to interact with some glycoproteins via sialic acid residues (see succinylated WGA).

Fluorescein labeled WGA has an appropriate number of fluorochromes bound to provide the optimum staining characteristics for this lectin. This conjugate is supplied essentially free of unconjugated fluorochromes. The excitation maximum is at 495 nm and the emission maximum is at 515 nm.

Specifications

Unit Size 5 mg, 10 mg
Applications Immunofluorescence, Glycobiology
Recommended Usage The recommended concentration range for use is 5-20 µg/ml.
Recommended Storage 2-8 °C
Maximum Excitation 495-500 nm
Maximum Emission 514-521 nm
Solution 10 mM HEPES, 0.15 M NaCl, pH 7.5, 0.08% sodium azide, 0.1 mM CaCl2
Concentration 5 mg active conjugate/ml
Conjugate Fluorescein
Color of Fluorescence Green
Sugar Specificity N-Acetylglucosamine

The post Wheat Germ Agglutinin (WGA), Fluorescein appeared first on VectorLabs.

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Description

The receptor sugar for WGA is N-acetylglucosamine, with preferential binding to dimers and trimers of this sugar. WGA can bind oligosaccharides containing terminal N-acetylglucosamine or chitobiose, structures which are common to many serum and membrane glycoproteins. Bacterial cell wall peptidoglycans, chitin, cartilage glycosaminoglycans, and glycolipids can also bind WGA. Native WGA has also been reported to interact with some glycoproteins via sialic acid residues (see succinylated WGA).

Fluorescein labeled WGA has an appropriate number of fluorochromes bound to provide the optimum staining characteristics for this lectin. This conjugate is supplied essentially free of unconjugated fluorochromes. The excitation maximum is at 495 nm and the emission maximum is at 515 nm.

Specifications

Unit Size5 mg, 10 mg
ApplicationsImmunofluorescence, Glycobiology
Recommended UsageThe recommended concentration range for use is 5-20 µg/ml.
Recommended Storage2-8 °C
Maximum Excitation495-500 nm
Maximum Emission514-521 nm
Solution10 mM HEPES, 0.15 M NaCl, pH 7.5, 0.08% sodium azide, 0.1 mM CaCl2
Concentration5 mg active conjugate/ml
ConjugateFluorescein
Color of FluorescenceGreen
Sugar SpecificityN-Acetylglucosamine

Documents

Citations

Technical Information

Wheat germ agglutinin (WGA) contains a group of closely related isolectins, with an isoelectric point about pH 9. The receptor sugar for WGA is N-acetylglucosamine, with preferential binding to dimers and trimers of this sugar. WGA can bind oligosaccharides containing terminal N-acetylglucosamine or chitobiose, structures which are common to many serum and membrane glycoproteins. Bacterial cell wall peptidoglycans, chitin, cartilage glycosaminoglycans, and glycolipids can also bind WGA. Native WGA has also been reported to interact with some glycoproteins via sialic acid residues (see succinylated WGA). This lectin is used for the purification of insulin receptors and for neuronal tracing.

Fluorescein labeled WGA has an appropriate number of fluorochromes bound to provide the optimum staining characteristics for this lectin. This conjugate is supplied essentially free of unconjugated fluorochromes. The excitation maximum is at 495 nm and the emission maximum is at 515 nm.

Accompanying each fluorescent lectin is an analysis data sheet summarizing the results of our quality control tests and providing pertinent information on the product. All of these reagents are supplied as solutions preserved with sodium azide.

Inhibiting/Eluting Sugar: Chitin Hydrolysate or 500 mM N-acetylglucosamine with salt and/or acid elution generally required

The post Wheat Germ Agglutinin (WGA), Fluorescein appeared first on VectorLabs.

]]> Streptavidin, Fluorescein https://staging.vectorlabs.com/products/fluorescein-streptavidin/ Fri, 28 Apr 2023 20:59:29 +0000 https://staging.vectorlabs.com/?post_type=product&p=13008 Description

Streptavidin, fluorescein (FITC) can be used to detect biotinylated secondary antibodies and other macromolecules in applications such as immunofluorescence, in situ hybridization, or flow cytometry. Amplification of fluorescent signals can be easily achieved with our highly purified fluorescein labeled streptavidin.

Features:

  • Recommended for routine immunofluorescence applications
  • Extremely high affinity for biotin
  • Has an optimal fluorochrome to protein ratio
  • Compared to conventional primary and secondary fluorescent techniques, can provide greater sensitivity and lower background staining
  • Excitation maximum at 495 nm
  • Emission maximum at about 515 nm
  • Color:  Green

Specifications

Unit Size 1 mg
Applications Immunofluorescence, In situ hybridization, Flow Cytometry/Cell Separation
Recommended Usage For diluting this product, we recommend a HEPES-, bicarbonate- or phosphate- buffered saline solution, approximately pH 8.2. Avoid using RPMI 1640 or other biotin-containing solutions as diluents. Serum also can contain biotin and should not be added to diluents. The recommended range of concentrations for staining of cells or tissues is 5-30 µg/ml.
Solution 10 mM HEPES, 0.15 M NaCl, pH 7.5, 0.08% sodium azide.
Maximum Excitation 495-500 nm
Maximum Emission 514-521 nm
Recommended Storage 2-8 °C
Concentration 1.0 mg active conjugate/ml
Conjugate Fluorescein
Color of Fluorescence Green
Format Concentrate

The post Streptavidin, Fluorescein appeared first on VectorLabs.

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Description

Streptavidin, fluorescein (FITC) can be used to detect biotinylated secondary antibodies and other macromolecules in applications such as immunofluorescence, in situ hybridization, or flow cytometry. Amplification of fluorescent signals can be easily achieved with our highly purified fluorescein labeled streptavidin.

Features:

  • Recommended for routine immunofluorescence applications
  • Extremely high affinity for biotin
  • Has an optimal fluorochrome to protein ratio
  • Compared to conventional primary and secondary fluorescent techniques, can provide greater sensitivity and lower background staining
  • Excitation maximum at 495 nm
  • Emission maximum at about 515 nm
  • Color:  Green

Specifications

Unit Size1 mg
ApplicationsImmunofluorescence, In situ hybridization, Flow Cytometry/Cell Separation
Recommended UsageFor diluting this product, we recommend a HEPES-, bicarbonate- or phosphate- buffered saline solution, approximately pH 8.2. Avoid using RPMI 1640 or other biotin-containing solutions as diluents. Serum also can contain biotin and should not be added to diluents. The recommended range of concentrations for staining of cells or tissues is 5-30 µg/ml.
Solution10 mM HEPES, 0.15 M NaCl, pH 7.5, 0.08% sodium azide.
Maximum Excitation495-500 nm
Maximum Emission514-521 nm
Recommended Storage2-8 °C
Concentration1.0 mg active conjugate/ml
ConjugateFluorescein
Color of FluorescenceGreen
FormatConcentrate

Documents

Citations

Technical Information

Vector Laboratories fluorochrome-conjugated streptavidin and avidin reagents are highly purified and possess very low non-specific binding properties. Using a biotin/avidin or biotin/streptavidin detection system results in an additional layer of amplification over a directly conjugated secondary antibody.

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]]> M.O.M.® (Mouse on Mouse) Immunodetection Kit, Fluorescein https://staging.vectorlabs.com/products/mouse-on-mouse-fluorescein-kit/ Tue, 25 Apr 2023 19:44:05 +0000 https://staging.vectorlabs.com/?post_type=product&p=12022 Description

The Vector M.O.M. Fluorescein Kit is specifically designed to reduce endogenous mouse IgG staining when using mouse primary antibodies on mouse tissue. It contains a proprietary Mouse IgG Blocking Reagent, a M.O.M. Biotinylated Anti-Mouse Ig Reagent, and Fluorescein Avidin DCS and is based on a simple and easy to follow avidin/biotin protocol.

Key advantages of the Vector M.O.M. Immunodetection Kits:

  • Clear, crisp specific staining of antigens of interest
  • Based on VECTASTAIN® ABC reagent protocols
  • Procedures are simple and easy to follow
  • No tedious calculations or antibody prebinding steps required

Kit Contents:

  • 6 ml of M.O.M. Protein Concentrate
  • 1 ml M.O.M. Mouse Ig Blocking Reagent
  • 0.1 ml M.O.M. Biotinylated Anti-Mouse IgG Reagent
  • 0.4 ml Fluorescein Avidin DCS

 

The Vector® M.O.M. Immunodetection Kit contains enough stock reagents to produce about 25 ml of working solution which is generally sufficient to stain approximately 250 tissue sections.

Specifications

Unit Size 1 kit
Applications Immunofluorescence
Technology ABC (Avidin/Biotin) Systems, Mouse on Mouse (M.O.M.) Systems
Conjugate Fluorescein
Color of Fluorescence Green
Target Species Mouse
Format Concentrate
Blocking Action Endogenous Mouse Ig

The post M.O.M.® (Mouse on Mouse) Immunodetection Kit, Fluorescein appeared first on VectorLabs.

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Description

The Vector M.O.M. Fluorescein Kit is specifically designed to reduce endogenous mouse IgG staining when using mouse primary antibodies on mouse tissue. It contains a proprietary Mouse IgG Blocking Reagent, a M.O.M. Biotinylated Anti-Mouse Ig Reagent, and Fluorescein Avidin DCS and is based on a simple and easy to follow avidin/biotin protocol.

Key advantages of the Vector M.O.M. Immunodetection Kits:

  • Clear, crisp specific staining of antigens of interest
  • Based on VECTASTAIN® ABC reagent protocols
  • Procedures are simple and easy to follow
  • No tedious calculations or antibody prebinding steps required

Kit Contents:

  • 6 ml of M.O.M. Protein Concentrate
  • 1 ml M.O.M. Mouse Ig Blocking Reagent
  • 0.1 ml M.O.M. Biotinylated Anti-Mouse IgG Reagent
  • 0.4 ml Fluorescein Avidin DCS

The Vector® M.O.M. Immunodetection Kit contains enough stock reagents to produce about 25 ml of working solution which is generally sufficient to stain approximately 250 tissue sections.

Specifications

Unit Size1 kit
ApplicationsImmunofluorescence
TechnologyABC (Avidin/Biotin) Systems, Mouse on Mouse (M.O.M.) Systems
ConjugateFluorescein
Color of FluorescenceGreen
Target SpeciesMouse
FormatConcentrate
Blocking ActionEndogenous Mouse Ig

Documents

Product FAQs

Vector Laboratories’ Mouse on Mouse detection kits (M.O.M.®) are designed for the detection of mouse IgG primary antibodies on mouse tissue sections. For detecting mouse primary antibodies on rat tissue, we offer a selection of anti-mouse, rat adsorbed secondary detection reagents. These are intended to be used for this application and would generate the most optimal signal to noise staining ratio. We offer a biotinylated anti-mouse IgG, rat adsorbed secondary antibody (Cat. No. BA-2001) for use with VECTASTAIN® ABC kits or avidin and streptavidin enzyme conjugates. Alternatively, we offer an ImmPRESS® HRP polymer anti-mouse IgG, rat adsorbed detection kit (Cat. No. MP-7422) for a convenient, one-step IHC methodology.

Citations

Technical Information

The inability of an anti-mouse secondary antibody to distinguish between a primary antibody produced in mice and the endogenous mouse immunoglobulins present in mouse tissue results in high background staining which obscures specific staining.  This problem can be essentially eliminated by using a Vector M.O.M. Kit.  The result is clear, crisp specific staining of the antigens of interest. Excellent staining results for a once difficult application have now become routine with the Vector M.O.M. System.

The Vector M.O.M. Immunodetection Kits are useful in any mouse-on-mouse application, such as studies in genetically engineered mice, including transgenic and knock-out models, as well as mouse xenografts.

The M.O.M. Kits can be also used in multiple antigen staining protocols to localize two different mouse primary antibodies on the same mouse tissue sections using fluorescent or enzyme-based detection systems. Detailed information for multiple antigen labeling can be found in our brochure, Discovery Through Color: A Guide to Multiple Antigen Labeling , available on our website.

Four Vector M.O.M. Kits are available. The three kits based on avidin/biotin technology use the same proprietary M.O.M. Mouse Ig Blocking Reagent and the M.O.M. Biotinylated Anti-Mouse Ig Reagent, but offer a choice of an enzyme-based or fluorescent-based visualization method. M.O.M. Mouse Ig Blocking Reagent and M.O.M. Biotinylated Anti-Mouse Ig Reagent are also available separately.

The Vector M.O.M. ImmPRESS® Kit contains our proprietary M.O.M. Mouse Ig Blocking Reagent paired with a specialized, ready-to-use, one-step, non-biotin M.O.M. ImmPRESS™ Peroxidase Polymer reagent.

The post M.O.M.® (Mouse on Mouse) Immunodetection Kit, Fluorescein appeared first on VectorLabs.

]]> Lectin Kit I, Fluorescein https://staging.vectorlabs.com/products/lectin-kit-i-fluorescein-labeled/ Tue, 25 Apr 2023 19:36:21 +0000 https://staging.vectorlabs.com/?post_type=product&p=12015 Description

Our lectin screening kits are designed to provide the investigator with a panel of seven lectins or lectin conjugates. The lectins have been selected to offer a variety of sugar specificities and are of the same high quality as the reagents offered individually.

Each Kit I (BK-1000, FLK-2100, RLK-2200) consists of 1 mg of the following lectins or lectin conjugates: Con A, DBA, PNA, RCA120 ,SBA, UEA I, WGA.

Specifications

Unit Size 1 kit
Applications Immunofluorescence, Glycobiology
Conjugate Fluorescein
Color of Fluorescence Green
Sugar Specificity Mannose, Galactose, Fucose, N-Acetylgalactosamine, N-Acetylglucosamine

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Description

Our lectin screening kits are designed to provide the investigator with a panel of seven lectins or lectin conjugates. The lectins have been selected to offer a variety of sugar specificities and are of the same high quality as the reagents offered individually.

Each Kit I (BK-1000, FLK-2100, RLK-2200) consists of 1 mg of the following lectins or lectin conjugates: Con A, DBA, PNA, RCA120 ,SBA, UEA I, WGA.

Specifications

Unit Size1 kit
ApplicationsImmunofluorescence, Glycobiology
ConjugateFluorescein
Color of FluorescenceGreen
Sugar SpecificityMannose, Galactose, Fucose, N-Acetylgalactosamine, N-Acetylglucosamine

Kit Contents:

This lectin screening kit is designed to provide the investigator with a panel of seven fluorescein labeled lectin conjugates. The lectins have been selected to offer a variety of sugar specificities and are of the same high quality as the reagents offered individually.

Kit I consists of 1 mg of the following fluorescein labeled lectin conjugates:

  • Con A (Concanavalin A; 2 mg/ml)
  • SBA (Glycine max (soybean) agglutinin; 2 mg/ml)
  • WGA (Triticum vulgaris (wheat germ) agglutinin; 2 mg/ml)
  • DBA (Dolichos biflorus agglutinin; 2 mg/ml)
  • UEA I (Ulex europaeus agglutinin I; 2 mg/ml)
  • RCA120 (Ricinus communis agglutinin; 2 mg/ml)
  • PNA (Arachis hypogaea (peanut) agglutinin; 2 mg/ml

These lectins are supplied essentially free of unconjugated fluorochrome and inactive lectin.

Fluorescein labeled lectin has an excitation maximum at 495 nm and an emission maximum at 515 nm.

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Technical Information

Our fluorescein labeled lectins are produced by using the highest quality fluorescein isothiocyanate, our affinity-purified lectins, and special conjugation procedures. The lectin conjugates have the appropriate number of fluorochromes bound which provide the maximum fluorescence and optimum staining characteristics for each particular lectin. These lectins are supplied essentially free of unconjugated fluorochrome and inactive lectin. Fluorescein labeled lectin has an excitation maximum at 495 nm and an emission maximum at 515 nm.

Accompanying each fluorescent lectin kit is an analysis data sheet summarizing the results of our quality control tests and providing pertinent information on the product. All of these reagents are supplied as solutions preserved with sodium azide.

Lectin screening kits jpg

The post Lectin Kit I, Fluorescein appeared first on VectorLabs.

]]> Aleuria Aurantia Lectin (AAL), Fluorescein https://staging.vectorlabs.com/products/fluorescein-aleuria-aurantia-lectin-aal/ Tue, 25 Apr 2023 19:33:01 +0000 https://staging.vectorlabs.com/?post_type=product&p=12004 Description

Unlike Ulex europaeus and Lotus tetragonolobus lectins which prefer (α -1,2) linked fucose residues, Aleuria aurantia lectin binds preferentially to fucose linked (α -1,6) to N-acetylglucosamine or to fucose linked (α -1,3) to N-acetyllactosamine related structures. AAL also reversibly binds fucose attached to nucleic acids.

Fluorescein labeled Aleuria aurantia lectin has an appropriate number of fluorochromes bound to provide the optimum staining characteristics for this lectin. This conjugate is supplied essentially free of unconjugated fluorochromes. The excitation maximum is at 495 nm and the emission maximum is at 515 nm.

Specifications

Unit Size 1 mg
Applications Immunofluorescence, Glycobiology
Recommended Usage The recommended concentration range for use is 5-20 µg/ml.
Recommended Storage 2-8°C
Maximum Excitation 495-500 nm
Inhibiting and/or Eluting Sugar 100 mM-400 mM L-fucose
Maximum Emission 514-521 nm
Solution 10 mM HEPES, pH 7.5, 0.15 M NaCl, 0.08% sodium azide.
Concentration 1 mg active conjugate/ml
Conjugate Fluorescein
Color of Fluorescence Green
Sugar Specificity Fucose, Arabinose

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Description

Unlike Ulex europaeus and Lotus tetragonolobus lectins which prefer (α -1,2) linked fucose residues, Aleuria aurantia lectin binds preferentially to fucose linked (α -1,6) to N-acetylglucosamine or to fucose linked (α -1,3) to N-acetyllactosamine related structures. AAL also reversibly binds fucose attached to nucleic acids.

Fluorescein labeled Aleuria aurantia lectin has an appropriate number of fluorochromes bound to provide the optimum staining characteristics for this lectin. This conjugate is supplied essentially free of unconjugated fluorochromes. The excitation maximum is at 495 nm and the emission maximum is at 515 nm.

Specifications

Unit Size1 mg
ApplicationsImmunofluorescence, Glycobiology
Recommended UsageThe recommended concentration range for use is 5-20 µg/ml.
Recommended Storage2-8°C
Maximum Excitation495-500 nm
Inhibiting and/or Eluting Sugar100 mM-400 mM L-fucose
Maximum Emission514-521 nm
Solution10 mM HEPES, pH 7.5, 0.15 M NaCl, 0.08% sodium azide.
Concentration1 mg active conjugate/ml
ConjugateFluorescein
Color of FluorescenceGreen
Sugar SpecificityFucose, Arabinose

Documents

Citations

Technical Information

This lectin is a dimer of two identical subunits of about 36 kDa each. 

Accompanying each fluorescent lectin is an analysis data sheet summarizing the results of our quality control tests and providing pertinent information on the product. All of these reagents are supplied as solutions preserved with sodium azide.

Inhibiting/Eluting Sugar: 100 mM L-fucose

The post Aleuria Aurantia Lectin (AAL), Fluorescein appeared first on VectorLabs.

]]> Wisteria Floribunda Lectin (WFA, WFL), Fluorescein https://staging.vectorlabs.com/products/fluorescein-wisteria-floribunda-lectin/ Tue, 25 Apr 2023 19:28:37 +0000 https://staging.vectorlabs.com/?post_type=product&p=11994 Description

The binding specificity of Wisteria floribunda lectin (WFL) is not completely clear but this lectin appears to preferentially bind carbohydrate structures terminating in N-acetylgalactosamine linked α or β to the 3 or 6 position of galactose. This lectin has been used to fractionate lymphocyte populations, and although not mitogenic, elicits the production of lymphokines from murine splenocytes.

Fluorescein labeled WFL has an appropriate number of fluorochromes bound to provide the optimum staining characteristics for this lectin. This conjugate is supplied essentially free of unconjugated fluorochromes.

  • Excitation maximum: 495 nm
  • Emission maximum: 515 nm
  • Color: Green

Specifications

Unit Size 2 mg
Applications Immunofluorescence, Glycobiology
Recommended Usage The recommended concentration range for use is 5-20 µg/ml.
Recommended Storage 2-8°C
Maximum Excitation 495-500 nm
Inhibiting and/or Eluting Sugar 200 mM N-acetylgalactosamine(S-9001)
Maximum Emission 514-521 nm
Solution 10 mM HEPES, 0.15 M NaCl, pH 7.5, 0.08% sodium azide. 0.1 mM CaCl2
Concentration 2 mg active conjugate/ml
Conjugate Fluorescein
Color of Fluorescence Green
Sugar Specificity N-Acetylgalactosamine

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Description

The binding specificity of Wisteria floribunda lectin (WFL) is not completely clear but this lectin appears to preferentially bind carbohydrate structures terminating in N-acetylgalactosamine linked α or β to the 3 or 6 position of galactose. This lectin has been used to fractionate lymphocyte populations, and although not mitogenic, elicits the production of lymphokines from murine splenocytes.

Fluorescein labeled WFL has an appropriate number of fluorochromes bound to provide the optimum staining characteristics for this lectin. This conjugate is supplied essentially free of unconjugated fluorochromes.

  • Excitation maximum: 495 nm
  • Emission maximum: 515 nm
  • Color: Green

Specifications

Unit Size2 mg
ApplicationsImmunofluorescence, Glycobiology
Recommended UsageThe recommended concentration range for use is 5-20 µg/ml.
Recommended Storage2-8°C
Maximum Excitation495-500 nm
Inhibiting and/or Eluting Sugar200 mM N-acetylgalactosamine(S-9001)
Maximum Emission514-521 nm
Solution10 mM HEPES, 0.15 M NaCl, pH 7.5, 0.08% sodium azide. 0.1 mM CaCl2
Concentration2 mg active conjugate/ml
ConjugateFluorescein
Color of FluorescenceGreen
Sugar SpecificityN-Acetylgalactosamine

Documents

Citations

Technical Information

Accompanying each fluorescent lectin is an analysis data sheet summarizing the results of our quality control tests and providing pertinent information on the product. All of these reagents are supplied as solutions preserved with sodium azide.

Inhibiting/Eluting Sugar: 200 mM N-acetylgalactosamine

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