Flow Cytometry – VectorLabs https://staging.vectorlabs.com From linker design and synthesis, bioconjugated design and manufacturing, biomolecule labeling and functionalization, and detection system and solutions, our team is here to help you move forward, with impact. Wed, 22 Oct 2025 17:11:11 +0000 en-US hourly 1 https://wordpress.org/?v=6.9 https://staging.vectorlabs.com/wp-content/uploads/2024/11/V-favicon-02-100x100.png Flow Cytometry – VectorLabs https://staging.vectorlabs.com 32 32 Streptavidin, CY5 https://staging.vectorlabs.com/products/cy5-streptavidin/ Thu, 04 May 2023 04:12:37 +0000 https://staging.vectorlabs.com/?post_type=product&p=15026 Description

Vector Laboratories fluorochrome-conjugated streptavidin and avidin reagents are highly purified and possess very low non-specific binding properties. They have extremely high affinity for biotin. These fluorescent conjugates can be used to detect biotinylated secondary antibodies and other macromolecules in applications such as immunofluorescence, in situ hybridization, or flow cytometry.

Amplification of fluorescent signals can be easily achieved with our biotinylated secondary antibodies followed by our highly purified fluorochrome-labeled streptavidin or avidin. Using a biotin/avidin or biotin/streptavidin detection system results in an additional layer of amplification over a directly conjugated secondary antibody.

  • Excitation:  650 nm
  • Emission:  670 nm
  • Color:  Far Red

Specifications

Unit Size 1 mg
Applications Immunofluorescence, In situ hybridization, Flow Cytometry/Cell Separation
Recommended Usage Recommended concentration range for use 5-30 µg/ml Avoid using RPMI 1640 or other biotin-containing solutions as diluents.Serum also can contain biotin and should not be added to diluents.
Solution 10 mM HEPES, 0.15 M NaCl, pH 7.5, 0.08% sodium azide.
Maximum Excitation 647 nm
Maximum Emission 663 nm
Recommended Storage 2-8 °C
Concentration 1.0 mg/ml active conjugate
Conjugate Cy5
Color of Fluorescence Far Red
Format Concentrate

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Description

Vector Laboratories fluorochrome-conjugated streptavidin and avidin reagents are highly purified and possess very low non-specific binding properties. They have extremely high affinity for biotin. These fluorescent conjugates can be used to detect biotinylated secondary antibodies and other macromolecules in applications such as immunofluorescence, in situ hybridization, or flow cytometry.

Amplification of fluorescent signals can be easily achieved with our biotinylated secondary antibodies followed by our highly purified fluorochrome-labeled streptavidin or avidin. Using a biotin/avidin or biotin/streptavidin detection system results in an additional layer of amplification over a directly conjugated secondary antibody. 

  • Excitation:  650 nm
  • Emission:  670 nm
  • Color:  Far Red

Specifications

Unit Size1 mg
ApplicationsImmunofluorescence, In situ hybridization, Flow Cytometry/Cell Separation
Recommended UsageRecommended concentration range for use 5-30 µg/ml Avoid using RPMI 1640 or other biotin-containing solutions as diluents.Serum also can contain biotin and should not be added to diluents.
Solution10 mM HEPES, 0.15 M NaCl, pH 7.5, 0.08% sodium azide.
Maximum Excitation647 nm
Maximum Emission663 nm
Recommended Storage2-8 °C
Concentration1.0 mg/ml active conjugate
ConjugateCy5
Color of FluorescenceFar Red
FormatConcentrate

Documents

Citations

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]]> Streptavidin, CY3 https://staging.vectorlabs.com/products/cy3-streptavidin/ Tue, 02 May 2023 19:24:51 +0000 https://staging.vectorlabs.com/?post_type=product&p=14518 Description

Vector Laboratories fluorochrome-conjugated streptavidin and avidin reagents are highly purified and possess very low non-specific binding properties. They have extremely high affinity for biotin. These fluorescent conjugates can be used to detect biotinylated secondary antibodies and other macromolecules in applications such as immunofluorescence, in situ hybridization, or flow cytometry.

Amplification of fluorescent signals can be easily achieved with our biotinylated secondary antibodies followed by our highly purified fluorochrome-labeled streptavidin or avidin. Using a biotin/avidin or biotin/streptavidin detection system results in an additional layer of amplification over a directly conjugated secondary antibody.

  • Excitation:  552 nm
  • Emission:  565 nm
  • Color:  Red

Specifications

Unit Size 1 mg
Applications Immunofluorescence, In situ hybridization, Flow Cytometry/Cell Separation
Recommended Usage Recommended concentration range for use 5-30 µg/ml Avoid using RPMI 1640 or other biotin-containing solutions as diluents. Serum also can contain biotin and should not be added to diluents.
Solution 10 mM HEPES, 0.15 M NaCl, pH 7.5, 0.08% sodium azide.
Maximum Excitation 550 nm
Maximum Emission 570 nm
Recommended Storage 2-8 °C
Concentration 1.0 mg/ml active conjugate
Conjugate Cy3
Color of Fluorescence Red
Format Concentrate

The post Streptavidin, CY3 appeared first on VectorLabs.

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Description

Vector Laboratories fluorochrome-conjugated streptavidin and avidin reagents are highly purified and possess very low non-specific binding properties. They have extremely high affinity for biotin. These fluorescent conjugates can be used to detect biotinylated secondary antibodies and other macromolecules in applications such as immunofluorescence, in situ hybridization, or flow cytometry.

Amplification of fluorescent signals can be easily achieved with our biotinylated secondary antibodies followed by our highly purified fluorochrome-labeled streptavidin or avidin. Using a biotin/avidin or biotin/streptavidin detection system results in an additional layer of amplification over a directly conjugated secondary antibody. 

  • Excitation:  552 nm
  • Emission:  565 nm
  • Color:  Red

Specifications

Unit Size1 mg
ApplicationsImmunofluorescence, In situ hybridization, Flow Cytometry/Cell Separation
Recommended UsageRecommended concentration range for use 5-30 µg/ml Avoid using RPMI 1640 or other biotin-containing solutions as diluents. Serum also can contain biotin and should not be added to diluents.
Solution10 mM HEPES, 0.15 M NaCl, pH 7.5, 0.08% sodium azide.
Maximum Excitation550 nm
Maximum Emission570 nm
Recommended Storage2-8 °C
Concentration1.0 mg/ml active conjugate
ConjugateCy3
Color of FluorescenceRed
FormatConcentrate

Documents

Citations

The post Streptavidin, CY3 appeared first on VectorLabs.

]]> Lycopersicon Esculentum (Tomato) Lectin (LEL, TL), Texas Red™ https://staging.vectorlabs.com/products/texas-red-lycopersicon-esculentum-lectin/ Tue, 02 May 2023 02:05:53 +0000 https://staging.vectorlabs.com/?post_type=product&p=13891 Description

Tomato lectin, although sharing some specificities with potato lectin, Datura lectin, and wheat germ agglutinin, has been reported to be dissimilar in many respects. LEL binds well to glycophorin and Tamm-Horsfall glycoprotein and has been used effectively to label vascular endothelium in rodents.

Texas Red® labeled Tomato lectin has an appropriate number of fluorochromes bound to provide the optimum staining characteristics for this lectin. This conjugate is supplied essentially free of unconjugated fluorochromes. The excitation maximum is at 595 nm and the emission maximum is at 615 nm.

Specifications

Unit Size 1 mg
Applications Immunofluorescence, Glycobiology
Recommended Usage If a precipitate forms upon long-term storage, warm to 37 ºC.
Recommended Storage 2-8 °C
Maximum Excitation 595-604 nm
Maximum Emission 606-615 nm
Solution 10 mM HEPES, 0.15 M NaCl, pH 7.5, 0.08% sodium azide 0.1 mM CaCl2.
Concentration 1 mg active conjugate/ml
Conjugate Texas Red
Color of Fluorescence Red
Sugar Specificity [GlcNAc]1-3, N-Acetylglucosamine

The post Lycopersicon Esculentum (Tomato) Lectin (LEL, TL), Texas Red™ appeared first on VectorLabs.

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Description

Tomato lectin, although sharing some specificities with potato lectin, Datura lectin, and wheat germ agglutinin, has been reported to be dissimilar in many respects. LEL binds well to glycophorin and Tamm-Horsfall glycoprotein and has been used effectively to label vascular endothelium in rodents.

Texas Red® labeled Tomato lectin has an appropriate number of fluorochromes bound to provide the optimum staining characteristics for this lectin. This conjugate is supplied essentially free of unconjugated fluorochromes. The excitation maximum is at 595 nm and the emission maximum is at 615 nm.

Specifications

Unit Size1 mg
ApplicationsImmunofluorescence, Glycobiology
Recommended UsageIf a precipitate forms upon long-term storage, warm to 37 ºC.
Recommended Storage2-8 °C
Maximum Excitation595-604 nm
Maximum Emission606-615 nm
Solution10 mM HEPES, 0.15 M NaCl, pH 7.5, 0.08% sodium azide 0.1 mM CaCl2.
Concentration1 mg active conjugate/ml
ConjugateTexas Red
Color of FluorescenceRed
Sugar Specificity[GlcNAc]1-3, N-Acetylglucosamine

Documents

Product FAQs

The Lycopersicon esculentum (tomato) lectin has been widely reported as an effective blood vessel marker for in vivo vascular perfusion studies in rodent species. Investigators have primarily utilized one of the fluorophore conjugated tomato lectin formats to trace blood vasculature in normal and diseased animals via tail vein or intracardiac injection. However, the biotinylated format has also been used. It allows for flexibility in subsequent visualization by way of either fluorescence or enzyme-based methods. Published references are best source for procedural details. Examples of references where biotinylated tomato lectins have been applied via in vivo perfusion are featured below: Robertson, R.T., et al. (2014) Histochem. Cell Biol. 143(2) Thurston, G., et al. (1998) Am. J Pathol. 153(4):1099-1112

Citations

Technical Information

Tomato lectin is a very stable single subunit glycoprotein containing about 50 percent arabinose and galactose and may form multimeric aggregates in solution. 

Tomato lectin (from Lycopersicon esculentum) is an effective marker of blood vessels and microglial cells in rodents. Conjugation of the lectin with a fluorophore facilitates fast, one-step detection and visualization using intravascular perfusion methods or direct application to tissue sections.

Texas Red® or DyLight™ 594 conjugated tomato lectin provides an excellent contrast to green/yellow fluorescence such as GFP expressed in transgenic animals, or with fluorescein conjugates in standard double label studies. The tomato lectin complements our existing range of lectin reagents and should be a valuable tool in examining rodent tumor angiogenesis, tracing neovascular development in xenograft models and brain research.

Accompanying each fluorescent lectin is an analysis data sheet summarizing the results of our quality control tests and providing pertinent information on the product. All of these reagents are supplied as solutions preserved with sodium azide.

Inhibiting/Eluting Sugar: Chitin Hydrolysate

The post Lycopersicon Esculentum (Tomato) Lectin (LEL, TL), Texas Red™ appeared first on VectorLabs.

]]> Horse Anti-Mouse IgG Antibody (H+L), Texas Red™ https://staging.vectorlabs.com/products/texas-red-horse-anti-mouse-igg/ Tue, 02 May 2023 01:43:13 +0000 https://staging.vectorlabs.com/?post_type=product&p=13884 Description

Texas Red Horse Anti-Mouse IgG Antibody can be used for immunofluorescence and other applications. Optimal F/P ratios have been established for each conjugate to ensure maximum fluorescence with minimal background staining.

Features:

  • Recognizes both heavy and light chains (H+L)
  • Optimally labeled with Texas Red to provide the brightest label for fluorescence microscopy
  • Supplied in solution
  • Excitation: 595 nm
  • Emission: 615 nm
  • Color: Red

Specifications

Unit Size 1.5 mg
Applications Immunofluorescence, In situ hybridization, Blotting Applications, Flow Cytometry/Cell Separation
Concentration 1.5 mg active conjugate/ml
Recommended Storage 2-8 °C
Solution 10 mM HEPES, 0.15 M NaCl, pH 7.5, 0.08% sodium azide
Maximum Emission 606-615 nm
Maximum Excitation 595-604 nm
Recommended Usage The recommended concentration range for use is 5-20 µg/ml. If this antibody is to be used in tissues which may contain cross-reacting endogenous immunoglobulins, dilution of this antibody may be made in buffers containing 2 % normal serum from the same species as the tissue.
Target Species Mouse
Conjugate Texas Red
Color of Fluorescence Red
Host Species Horse
Format Concentrate

The post Horse Anti-Mouse IgG Antibody (H+L), Texas Red™ appeared first on VectorLabs.

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Description

Texas Red Horse Anti-Mouse IgG Antibody can be used for immunofluorescence and other applications. Optimal F/P ratios have been established for each conjugate to ensure maximum fluorescence with minimal background staining.

Features:

  • Recognizes both heavy and light chains (H+L) 
  • Optimally labeled with Texas Red to provide the brightest label for fluorescence microscopy 
  • Supplied in solution 
  • Excitation: 595 nm 
  • Emission: 615 nm 
  • Color: Red

Specifications

Unit Size1.5 mg
ApplicationsImmunofluorescence, In situ hybridization, Blotting Applications, Flow Cytometry/Cell Separation
Concentration1.5 mg active conjugate/ml
Recommended Storage2-8 °C
Solution10 mM HEPES, 0.15 M NaCl, pH 7.5, 0.08% sodium azide
Maximum Emission606-615 nm
Maximum Excitation595-604 nm
Recommended UsageThe recommended concentration range for use is 5-20 µg/ml. If this antibody is to be used in tissues which may contain cross-reacting endogenous immunoglobulins, dilution of this antibody may be made in buffers containing 2 % normal serum from the same species as the tissue.
Target SpeciesMouse
ConjugateTexas Red
Color of FluorescenceRed
Host SpeciesHorse
FormatConcentrate

Documents

 

Citations

Technical Information

The anti-mouse Ig antibodies are prepared by hyperimmunizing animals in a manner that produces high affinity antibodies. These are then purified by an affinity chromatography procedure designed to remove any low affinity antibodies which may be present. Cross-reactivities that are likely to interfere with specific labeling are removed by solid-phase adsorption techniques. The final product is then subjected to rigorous quality control assays including immunodiffusion, solid-phase enzyme immunoassays, gel electrophoresis and solid-phase binding assays. In preparing the labeled antibodies, great care is taken to ensure the maximum degree of labeling with no alteration in the specificity and affinity of the antibody. The labeled antibody then undergoes a further series of quality control assays, including immunohistochemical analysis. 

Texas Red (sulforhodamine 101) produces a bright red fluorescence well removed from the emission spectrum of fluorescein. Texas Red Horse Anti-Mouse IgG Antibody conjugate is ideal as a single or double label reagent for flow cytometry or tissue staining.

The post Horse Anti-Mouse IgG Antibody (H+L), Texas Red™ appeared first on VectorLabs.

]]> Goat Anti-Rabbit IgG Antibody (H+L), Texas Red™ https://staging.vectorlabs.com/products/texas-red-goat-anti-rabbit-igg/ Tue, 02 May 2023 01:40:33 +0000 https://staging.vectorlabs.com/?post_type=product&p=13877 Description

The Texas Red Goat Anti-Rabbit IgG Antibody can be used for immunofluorescence and other applications. Optimal F/P ratios have been established for each conjugate to ensure maximum fluorescence with minimal background staining.

Features:

  • Recognizes both heavy and light chains (H+L)
  • Optimally labeled with Texas Red to provide the brightest label for fluorescence microscopy
  • Supplied in solution
  • Excitation: 595 nm
  • Emission: 615 nm
  • Color: Red

Specifications

Unit Size 1.5 mg
Applications Immunofluorescence, In situ hybridization, Blotting Applications, Flow Cytometry/Cell Separation
Concentration 1.5 mg active conjugate/ml
Recommended Storage 2-8 °C
Solution 10 mM HEPES, 0.15 M NaCl, pH 7.5, 0.08% sodium azide
Maximum Emission 606-615 nm
Maximum Excitation 595-604 nm
Recommended Usage The recommended concentration range for use is 5-20 µg/ml.If this antibody is to be used in tissues which may contain cross-reacting endogenous immunoglobulins, dilution of this antibody may be made in buffers containing 2 % normal serum from the same species as the tissue.
Target Species Rabbit
Conjugate Texas Red
Color of Fluorescence Red
Host Species Goat
Format Concentrate

The post Goat Anti-Rabbit IgG Antibody (H+L), Texas Red™ appeared first on VectorLabs.

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Description

The Texas Red Goat Anti-Rabbit IgG Antibody can be used for immunofluorescence and other applications. Optimal F/P ratios have been established for each conjugate to ensure maximum fluorescence with minimal background staining.

Features:

  • Recognizes both heavy and light chains (H+L)
  • Optimally labeled with Texas Red to provide the brightest label for fluorescence microscopy
  • Supplied in solution
  • Excitation: 595 nm
  • Emission: 615 nm
  • Color: Red

Specifications

Unit Size1.5 mg
ApplicationsImmunofluorescence, In situ hybridization, Blotting Applications, Flow Cytometry/Cell Separation
Concentration1.5 mg active conjugate/ml
Recommended Storage2-8 °C
Solution10 mM HEPES, 0.15 M NaCl, pH 7.5, 0.08% sodium azide
Maximum Emission606-615 nm
Maximum Excitation595-604 nm
Recommended UsageThe recommended concentration range for use is 5-20 µg/ml.If this antibody is to be used in tissues which may contain cross-reacting endogenous immunoglobulins, dilution of this antibody may be made in buffers containing 2 % normal serum from the same species as the tissue.
Target SpeciesRabbit
ConjugateTexas Red
Color of FluorescenceRed
Host SpeciesGoat
FormatConcentrate

Documents

Citations

Technical Information

The goat anti-rabbit Ig antibodies are prepared by hyperimmunizing animals in a manner that produces high affinity antibodies. These are then purified by an affinity chromatography procedure designed to remove any low affinity antibodies which may be present. Cross-reactivities that are likely to interfere with specific labeling are removed by solid-phase adsorption techniques. The final product is then subjected to rigorous quality control assays including immunodiffusion, solid-phase enzyme immunoassays, gel electrophoresis and solid-phase binding assays. In preparing the labeled antibodies, great care is taken to ensure the maximum degree of labeling with no alteration in the specificity and affinity of the antibody. The labeled antibody then undergoes a further series of quality control assays, including immunohistochemical analysis. 

Texas Red (sulforhodamine 101) excites at about 595 nm and emits at about 615 nm, producing a bright red fluorescence well removed from the emission spectrum of fluorescein. Texas Red Goat Anti-Rabbit IgG (H+L) Antibody conjugate is ideal as a single or double label reagent for flow cytometry or tissue staining.

The post Goat Anti-Rabbit IgG Antibody (H+L), Texas Red™ appeared first on VectorLabs.

]]> Bovine Serum Albumin (BSA), Immunohistochemical Grade https://staging.vectorlabs.com/products/bovine-serum-albumin-immunohistochemical-grade/ Tue, 02 May 2023 01:32:56 +0000 https://staging.vectorlabs.com/?post_type=product&p=13862 Description

This ultrapure grade of bovine serum albumin (BSA) can be used as a diluent or a blocking agent.  It is free of impurities present in other grades of BSA which can introduce artifacts or increase background in immunohistochemical staining, ELISAs, or blot development.

Specifications

Unit Size 500 mg
Applications Immunohistochemistry / Immunocytochemistry, Immunofluorescence, In situ hybridization, Blotting Applications, Elispot, ELISAs, Flow Cytometry/Cell Separation
Recommended Storage 2-8 °C
Blocking Action Non-Specific Protein Blocking
Format Lyophilized

The post Bovine Serum Albumin (BSA), Immunohistochemical Grade appeared first on VectorLabs.

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Description

This ultrapure grade of bovine serum albumin (BSA) can be used as a diluent or a blocking agent.  It is free of impurities present in other grades of BSA which can introduce artifacts or increase background in immunohistochemical staining, ELISAs, or blot development.

Specifications

Unit Size500 mg
ApplicationsImmunohistochemistry / Immunocytochemistry, Immunofluorescence, In situ hybridization, Blotting Applications, Elispot, ELISAs, Flow Cytometry/Cell Separation
Recommended Storage2-8 °C
Blocking ActionNon-Specific Protein Blocking
FormatLyophilized

Documents

Citations

The post Bovine Serum Albumin (BSA), Immunohistochemical Grade appeared first on VectorLabs.

]]> Streptavidin, DyLight™ 594 https://staging.vectorlabs.com/products/dylight-594-streptavidin/ Mon, 01 May 2023 19:42:16 +0000 https://staging.vectorlabs.com/?post_type=product&p=13589 Description

Features:

  • Can be used to detect biotinylated secondary antibodies and other macromolecules in applications such as immunofluorescence, in situ hybridization, or flow cytometry
  • Highly purified and possess very low non-specific binding properties
  • Extremely high affinity for biotin
  • Advantages of DyLight dyes include greater photostability, pH independence, and brighter fluorescence
  • Excitation at 592 nm
  • Emission at 617 nm
  • Color:  Red

Specifications

Unit Size 1 mg
Applications Immunofluorescence, In situ hybridization, Flow Cytometry/Cell Separation
Recommended Usage Recommended concentration range for use: 5-30 µg/ml. Avoid using RPMI 1640 or other biotin-containing solutions as diluents. Serum also can contain biotin and should not be added to diluents.
Solution 10 mM HEPES, 0.15 M NaCl, pH 7.5, 0.08% sodium azide.
Maximum Excitation 592 nm
Maximum Emission 617 nm
Recommended Storage 2-8 °C
Concentration 1.0 mg/ml active conjugate
Conjugate DyLight 594
Color of Fluorescence Red
Format Concentrate

The post Streptavidin, DyLight™ 594 appeared first on VectorLabs.

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Description

Features:

  • Can be used to detect biotinylated secondary antibodies and other macromolecules in applications such as immunofluorescence, in situ hybridization, or flow cytometry
  • Highly purified and possess very low non-specific binding properties
  • Extremely high affinity for biotin
  • Advantages of DyLight dyes include greater photostability, pH independence, and brighter fluorescence
  • Excitation at 592 nm
  • Emission at 617 nm
  • Color:  Red

Specifications

Unit Size1 mg
ApplicationsImmunofluorescence, In situ hybridization, Flow Cytometry/Cell Separation
Recommended UsageRecommended concentration range for use: 5-30 µg/ml. Avoid using RPMI 1640 or other biotin-containing solutions as diluents. Serum also can contain biotin and should not be added to diluents.
Solution10 mM HEPES, 0.15 M NaCl, pH 7.5, 0.08% sodium azide.
Maximum Excitation592 nm
Maximum Emission617 nm
Recommended Storage2-8 °C
Concentration1.0 mg/ml active conjugate
ConjugateDyLight 594
Color of FluorescenceRed
FormatConcentrate

Documents

Citations

Technical Information

Vector Laboratories fluorochrome-conjugated streptavidin and avidin reagents are highly purified and possess very low non-specific binding properties. They have extremely high affinity for biotin. These fluorescent conjugates can be used to detect biotinylated secondary antibodies and other macromolecules in applications such as immunofluorescence, in situ hybridization, or flow cytometry.

Amplification of fluorescent signals can be easily achieved with our biotinylated secondary antibodies followed by our highly purified fluorochrome-labeled streptavidin or avidin. Using a biotin/avidin or biotin/streptavidin detection system results in an additional layer of amplification over a directly conjugated secondary antibody.

DyLight dyes offer several advantages including greater photostability, pH independence, and brighter fluorescence. We offer DyLight conjugated streptavidin for use in a variety of applications, in particular, cell- and tissue-based immunofluorescent detection. The DyLight conjugates are completely stable from pH 4 to pH 9, making them compatible with many aqueous-based buffers and diluents.

DyLightspectra jpg

Dylight Table1

The post Streptavidin, DyLight™ 594 appeared first on VectorLabs.

]]> Streptavidin, DyLight® 488 https://staging.vectorlabs.com/products/dylight-488-streptavidin/ Mon, 01 May 2023 19:35:55 +0000 https://staging.vectorlabs.com/?post_type=product&p=13562 Description

DyLight 488 Streptavidin can be used to detect biotinylated secondary antibodies and other macromolecules in applications such as immunofluorescence, in situ hybridization, or flow cytometry.

Features:

  • Advantages of DyLight™ dyes include greater photostability, pH independence, and brighter fluorescence
  • Excitation maximum: 493 nm
  • Emission maximum: 518 nm
  • Color:  Green

Specifications

Unit Size 1 mg
Applications Immunofluorescence, In situ hybridization, Flow Cytometry/Cell Separation
Recommended Usage Recommended concentration range for use: 5-30 µg/ml. Avoid using RPMI 1640 or other biotin-containing solutions as diluents. Serum also can contain biotin and should not be added to diluents.
Solution 10 mM HEPES, 0.15 M NaCl, pH 7.5, 0.08% sodium azide.
Maximum Excitation 493 nm
Maximum Emission 518 nm
Recommended Storage 2-8 °C
Concentration 1.0 mg/ml active conjugate
Conjugate DyLight 488
Color of Fluorescence Green
Format Concentrate

The post Streptavidin, DyLight® 488 appeared first on VectorLabs.

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Description

DyLight 488 Streptavidin can be used to detect biotinylated secondary antibodies and other macromolecules in applications such as immunofluorescence, in situ hybridization, or flow cytometry. 

Features:

  • Advantages of DyLight™ dyes include greater photostability, pH independence, and brighter fluorescence
  • Excitation maximum: 493 nm
  • Emission maximum: 518 nm
  • Color:  Green

Specifications

Unit Size1 mg
ApplicationsImmunofluorescence, In situ hybridization, Flow Cytometry/Cell Separation
Recommended UsageRecommended concentration range for use: 5-30 µg/ml. Avoid using RPMI 1640 or other biotin-containing solutions as diluents. Serum also can contain biotin and should not be added to diluents.
Solution10 mM HEPES, 0.15 M NaCl, pH 7.5, 0.08% sodium azide.
Maximum Excitation493 nm
Maximum Emission518 nm
Recommended Storage2-8 °C
Concentration1.0 mg/ml active conjugate
ConjugateDyLight 488
Color of FluorescenceGreen
FormatConcentrate

Documents

Citations

Technical Information

Vector Laboratories fluorochrome-conjugated streptavidin and avidin reagents are highly purified and possess very low non-specific binding properties. They have extremely high affinity for biotin. 

Amplification of fluorescent signals can be easily achieved with our biotinylated secondary antibodies followed by our highly purified fluorochrome-labeled streptavidin or avidin. Using a biotin/avidin or biotin/streptavidin detection system results in an additional layer of amplification over a directly conjugated secondary antibody.

DyLight™ dyes offer several advantages including greater photostability, pH independence, and brighter fluorescence. We offer DyLight™ conjugated streptavidin for use in a variety of applications, in particular, cell- and tissue-based immunofluorescent detection. The DyLight™ conjugates are completely stable from pH 4 to pH 9, making them compatible with many aqueous-based buffers and diluents.

DyLightspectra jpg

Dylight Table1

The post Streptavidin, DyLight® 488 appeared first on VectorLabs.

]]> Goat Anti-Digoxigenin/Digoxin (DIG) Antibody, DyLight™ 488 https://staging.vectorlabs.com/products/dylight-488-anti-digoxigenin-digoxin-dig/ Mon, 01 May 2023 19:02:49 +0000 https://staging.vectorlabs.com/?post_type=product&p=13538 Description

Digoxigenin (DIG) is a small plant-derived molecule not found in animals. DIG is used to label nucleic acid probes for applications such as in situ hybridization. DIG-labeled probes are detected with antibodies specifically directed against the DIG label. We have generated high affinity and highly purified antibodies for this purpose. The DyLight™ conjugated antibodies allow a one-step fluorescent visualization of DIG-labeled probes.

Features:

  • High affinity and highly purified antibody to detect DIG-labed probes
  • DyLight® 488 conjugated antibodies allow a one-step fluorescent visualization of DIG-labeled probes
  • Excitation:  493 nm
  • Emission:  518 nm
  • Color:  Green

Specifications

Unit Size 0.5 mg
Applications In situ hybridization
Concentration 1.0 mg active conjugate/ml
Recommended Storage 2-8 °C
Solution 10 mM HEPES, 0.15 M NaCl, pH 7.8, 0.08% sodium azide.
Maximum Emission 518 nm
Maximum Excitation 493 nm
Recommended Usage The recommended concentration range for use is 5-20 µg/ml.
Detection Target Digoxigenin/Digoxin (DIG)
Conjugate DyLight 488
Color of Fluorescence Green
Host Species Goat
Format Concentrate

The post Goat Anti-Digoxigenin/Digoxin (DIG) Antibody, DyLight™ 488 appeared first on VectorLabs.

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Description

Digoxigenin (DIG) is a small plant-derived molecule not found in animals. DIG is used to label nucleic acid probes for applications such as in situ hybridization. DIG-labeled probes are detected with antibodies specifically directed against the DIG label. We have generated high affinity and highly purified antibodies for this purpose. The DyLight™ conjugated antibodies allow a one-step fluorescent visualization of DIG-labeled probes.

Features:

  • High affinity and highly purified antibody to detect DIG-labed probes
  • DyLight® 488 conjugated antibodies allow a one-step fluorescent visualization of DIG-labeled probes
  • Excitation:  493 nm
  • Emission:  518 nm
  • Color:  Green

Specifications

Unit Size0.5 mg
ApplicationsIn situ hybridization
Concentration1.0 mg active conjugate/ml
Recommended Storage2-8 °C
Solution10 mM HEPES, 0.15 M NaCl, pH 7.8, 0.08% sodium azide.
Maximum Emission518 nm
Maximum Excitation493 nm
Recommended UsageThe recommended concentration range for use is 5-20 µg/ml.
Detection TargetDigoxigenin/Digoxin (DIG)
ConjugateDyLight 488
Color of FluorescenceGreen
Host SpeciesGoat
FormatConcentrate

Documents

Citations

Technical Information

DyLight™ dyes offer several advantages including greater photostability, pH independence and brighter fluorescence. DyLight™ conjugates are completely stable from pH 4 to pH 9, making them compatible with many buffers and diluents.

The post Goat Anti-Digoxigenin/Digoxin (DIG) Antibody, DyLight™ 488 appeared first on VectorLabs.

]]> Streptavidin, DyLight™ 549 https://staging.vectorlabs.com/products/dylight-549-streptavidin/ Sun, 30 Apr 2023 03:48:22 +0000 https://staging.vectorlabs.com/?post_type=product&p=13465 Description

Vector Laboratories fluorochrome-conjugated streptavidin and avidin reagents are highly purified and possess very low non-specific binding properties. They have extremely high affinity for biotin. These fluorescent conjugates can be used to detect biotinylated secondary antibodies and other macromolecules in applications such as immunofluorescence, in situ hybridization, or flow cytometry.

Features:

  • Highly purified and possess very low non-specific binding properties
  • Excitation at 556 nm
  • Emission at 571 nm
  • Color:  Orange

Specifications

Unit Size 1 mg
Applications Immunofluorescence, In situ hybridization, Flow Cytometry/Cell Separation
Recommended Usage 5-30 µg/ml. Avoid using RPMI 1640 or other biotin-containing solutions as diluents. Serum also can contain biotin and should not be added to diluents.
Solution 10 mM HEPES, 0.15 M NaCl, pH 7.5, 0.08% sodium azide.
Maximum Excitation 556 nm
Maximum Emission 571 nm
Recommended Storage 2-8 °C
Concentration 1.0 mg/ml active conjugate
Conjugate DyLight 549
Color of Fluorescence Orange
Format Concentrate

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Description

Vector Laboratories fluorochrome-conjugated streptavidin and avidin reagents are highly purified and possess very low non-specific binding properties. They have extremely high affinity for biotin. These fluorescent conjugates can be used to detect biotinylated secondary antibodies and other macromolecules in applications such as immunofluorescence, in situ hybridization, or flow cytometry.

Features:

  • Highly purified and possess very low non-specific binding properties
  • Excitation at 556 nm
  • Emission at 571 nm
  • Color:  Orange

Specifications

Unit Size1 mg
ApplicationsImmunofluorescence, In situ hybridization, Flow Cytometry/Cell Separation
Recommended Usage5-30 µg/ml. Avoid using RPMI 1640 or other biotin-containing solutions as diluents. Serum also can contain biotin and should not be added to diluents.
Solution10 mM HEPES, 0.15 M NaCl, pH 7.5, 0.08% sodium azide.
Maximum Excitation556 nm
Maximum Emission571 nm
Recommended Storage2-8 °C
Concentration1.0 mg/ml active conjugate
ConjugateDyLight 549
Color of FluorescenceOrange
FormatConcentrate

Documents

Citations

Technical Information

Amplification of fluorescent signals can be easily achieved with our biotinylated secondary antibodies followed by our highly purified fluorochrome-labeled streptavidin or avidin. Using a biotin/avidin or biotin/streptavidin detection system results in an additional layer of amplification over a directly conjugated secondary antibody.

DyLight™ dyes offer several advantages including greater photostability, pH independence, and brighter fluorescence. We offer DyLight™ conjugated streptavidin for use in a variety of applications, in particular, cell- and tissue-based immunofluorescent detection. The DyLight™ conjugates are completely stable from pH 4 to pH 9, making them compatible with many aqueous-based buffers and diluents.

DyLightspectra jpg

Dylight Table1

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