Elispot – VectorLabs https://staging.vectorlabs.com From linker design and synthesis, bioconjugated design and manufacturing, biomolecule labeling and functionalization, and detection system and solutions, our team is here to help you move forward, with impact. Wed, 22 Oct 2025 17:15:30 +0000 en-US hourly 1 https://wordpress.org/?v=6.9 https://staging.vectorlabs.com/wp-content/uploads/2024/11/V-favicon-02-100x100.png Elispot – VectorLabs https://staging.vectorlabs.com 32 32 TMB Substrate Kit, Peroxidase (HRP), (3,3′, 5,5′-tetramethylbenzidine) https://staging.vectorlabs.com/products/tmb-hrp-3-3-5-5-tetramethylbenzidine/ Sun, 21 May 2023 01:21:26 +0000 https://staging.vectorlabs.com/?post_type=product&p=17687 Description

TMB (3,3′, 5,5′ tetramethylbenzidine dihydrochloride) is a sensitive chromogen substrate for peroxidase and a non-carcinogenic substitute for benzidine. TMB can be used for blotting, immunohistochemical, and in situ hybridization applications. Slides stained with TMB can be dehydrated and permanently mounted and the soluble blue product, produced in the absence of the stabilizing solution, is useful for ELISA assays.

The TMB HRP Substrate Kit (SK-4400) contains the reagents needed to produce either a precipitating blue reaction product or a soluble blue reaction product. This kit also contains a stabilizing solution which, when added to the working solution, allows the development of a blue precipitate. Kit materials are pre-packaged in convenient dropper bottles.

Features:

  • Greater sensitivity than conventional substrates
  • Stock solutions supplied in convenient dropper bottles promoting ease of handling
  • No wait times for mixing and dissolving powders or tablets
  • Sufficient reagents to produce 300 ml of working solution

Specifications

Unit Size 1 kit
Applications Immunohistochemistry / Immunocytochemistry, In situ hybridization, Blotting Applications, Elispot, ELISAs
Detection Enzyme(s) Peroxidase
Substrate Reaction Product Precipitating Reaction Product
Chromogen Color Blue
Microscopy Brightfield, Spectral Imaging
Mounting Non-Aqueous (Permanent)
Safety Title WARNING: Cancer – www.P65Warnings.ca.gov

What’s in the box:

  • 6 ml TMB Reagent 1
  • 6 ml TMB Reagent 2
  • 6 ml TMB Reagent 3
  • 6 ml TMB Reagent 4

The post TMB Substrate Kit, Peroxidase (HRP), (3,3′, 5,5′-tetramethylbenzidine) appeared first on VectorLabs.

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Description

TMB (3,3′, 5,5′ tetramethylbenzidine dihydrochloride) is a sensitive chromogen substrate for peroxidase and a non-carcinogenic substitute for benzidine. TMB can be used for blotting, immunohistochemical, and in situ hybridization applications. Slides stained with TMB can be dehydrated and permanently mounted and the soluble blue product, produced in the absence of the stabilizing solution, is useful for ELISA assays.

The TMB HRP Substrate Kit (SK-4400) contains the reagents needed to produce either a precipitating blue reaction product or a soluble blue reaction product. This kit also contains a stabilizing solution which, when added to the working solution, allows the development of a blue precipitate. Kit materials are pre-packaged in convenient dropper bottles.

Features:

  • Greater sensitivity than conventional substrates
  • Stock solutions supplied in convenient dropper bottles promoting ease of handling
  • No wait times for mixing and dissolving powders or tablets
  • Sufficient reagents to produce 300 ml of working solution

Specifications

Unit Size1 kit
ApplicationsImmunohistochemistry / Immunocytochemistry, In situ hybridization, Blotting Applications, Elispot, ELISAs
Detection Enzyme(s)Peroxidase
Substrate Reaction ProductPrecipitating Reaction Product
Chromogen ColorBlue
MicroscopyBrightfield, Spectral Imaging
MountingNon-Aqueous (Permanent)
Safety TitleWARNING: Cancer – www.P65Warnings.ca.gov

What’s in the box:

  • 6 ml TMB Reagent 1
  • 6 ml TMB Reagent 2
  • 6 ml TMB Reagent 3
  • 6 ml TMB Reagent 4

Documents

Technical Information

TMB (3,3′, 5,5′ tetramethylbenzidine dihydrochloride) is a sensitive chromogen substrate for peroxidase and a non-carcinogenic substitute for benzidine. TMB can be used for blotting, immunohistochemical, and in situ hybridization applications. Slides stained with TMB can be dehydrated and permanently mounted and the soluble blue product, produced in the absence of the stabilizing solution, is useful for ELISA assays.

Counterstain / Substrate Compatibility

This table is designed as a reference to determine the optimal counterstain / substrate combination for your application. Consideration should be given to tissue type, antigen unmasking protocol, and other detection parameters to achieve the desired staining intensity.

SubstrateCatalog NumberHematoxylin (H-3404)
Hematoxylin QS (H-3401)		Methyl Green (H-3402)Nuclear Fast Red (H-3404)
ImmPACT DAB (brown)SK-4105Excellent ContrastExcellent ContrastFair Contrast
ImmPACT DAB EqV (brown)SK-4103Excellent ContrastExcellent ContrastFair Contrast
DAB (brown)SK-4100Excellent ContrastExcellent ContrastFair Contrast
DAB-Ni (gray-black)SK-4100Excellent ContrastFair Contrast*Good Contrast
ImmPACT AEC (red)SK-4205Excellent ContrastCounterstain Incompatibility**Color Incompatibility
ImmPACT AMEC Red (red)SK-4285Excellent ContrastCounterstain Incompatibility**Color Incompatibility
AEC (red)SK-4200Excellent ContrastCounterstain Incompatibility**Color Incompatibility
TMB (blue)SK-4400Color IncompatibilityCounterstain IncompatibilityExcellent Contrast
ImmPACT VIP (purple)SK-4605Fair ContrastExcellent ContrastPoor Contrast
Vector VIP (purple)SK-4600Fair ContrastExcellent ContrastPoor Contrast
ImmPACT SG (blue-gray)SK-4705Poor ContrastGood ContrastExcellent Contrast
SG (blue-gray)SK-4700Poor ContrastGood ContrastExcellent Contrast
ImmPACT NovaRED (red)SK-4805Excellent ContrastExcellent Contrast***Color Incompatibility
Vector NovaRED (red)SK-4800Excellent ContrastExcellent Contrast***Color Incompatibility
ImmPACT Vector Red (magenta)SK-5105Excellent ContrastExcellent ContrastColor Incompatibility
Vector Red (magenta)SK-5100Excellent ContrastExcellent ContrastColor Incompatibility
Vector Black (black)SK-5200Excellent ContrastExcellent Contrast*Excellent Contrast
Vector Blue (blue)SK-5300Color IncompatibilityGood ContrastExcellent Contrast
BCIP / NBT (indigo)SK-5400Color IncompatibilityExcellent Contrast*Excellent Contrast
*This substrate shows a slight decrease in sensitivity following the methyl green protocol. This decrease can be minimized by reducing the heat incubation and acetone rinse times.
**Substrate dissolves in acetone wash.
***A slight color change in ImmPACT NovaRED and Vector NovaRED reaction produt may be seen using methyl green.

SUBSTRATEsensitivity2 jpg

The post TMB Substrate Kit, Peroxidase (HRP), (3,3′, 5,5′-tetramethylbenzidine) appeared first on VectorLabs.

]]> Animal-Free Blocker® and Diluent, R.T.U. https://staging.vectorlabs.com/products/animal-free-block-and-diluent-afb/ Thu, 04 May 2023 04:53:03 +0000 https://staging.vectorlabs.com/?post_type=product&p=15151 Description

R.T.U. Animal Free Blocker and Diluent is a ready-to-use solution designed to be used as an antibody diluent and a blocking reagent to stabilize diluted antibody solutions and diminish background due to non-specific binding in immunohistochemical applications. This product contains no animal-derived proteins. The R.T.U. Animal Free Blocker and Diluent is especially convenient in multiplex IHC (multiple antigen labeling) applications where antibodies from several different species are used.

Specifications

Unit Size 100 ml
Applications Immunohistochemistry / Immunocytochemistry, Immunofluorescence, Blotting Applications, Elispot
Recommended Storage 2-8 °C
Recommended Usage This product is designed to be used as an antibody diluent and a blocking reagent to stabilize diluted antibody solutions and diminish background due to non-specific binding in immunohistochemical applications. This reagent contains no animal-derived proteins and can be used as an alternative to sera, BSA, casein or non-fat dry milk in situations when animal protein-based blocking solutions cause increased background or interfere with blot development. Primary and secondary antibodies can be diluted in this solution and stored at 4 ºC for up to a week.
Blocking Action Non-Specific Protein Blocking
Format Ready-to-Use

The post Animal-Free Blocker® and Diluent, R.T.U. appeared first on VectorLabs.

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Description

R.T.U. Animal Free Blocker and Diluent is a ready-to-use solution designed to be used as an antibody diluent and a blocking reagent to stabilize diluted antibody solutions and diminish background due to non-specific binding in immunohistochemical applications. This product contains no animal-derived proteins. The R.T.U. Animal Free Blocker and Diluent is especially convenient in multiplex IHC (multiple antigen labeling) applications where antibodies from several different species are used. 

Specifications

Unit Size100 ml
ApplicationsImmunohistochemistry / Immunocytochemistry, Immunofluorescence, Blotting Applications, Elispot
Recommended Storage2-8 °C
Recommended UsageThis product is designed to be used as an antibody diluent and a blocking reagent to stabilize diluted antibody solutions and diminish background due to non-specific binding in immunohistochemical applications. This reagent contains no animal-derived proteins and can be used as an alternative to sera, BSA, casein or non-fat dry milk in situations when animal protein-based blocking solutions cause increased background or interfere with blot development. Primary and secondary antibodies can be diluted in this solution and stored at 4 ºC for up to a week.
Blocking ActionNon-Specific Protein Blocking
FormatReady-to-Use

Documents

Product FAQs

Both products are derived from the same plant protein and in some applications can be used interchangeably. At a high level, one is presented as a ready-to-use (RTU) format and the other a more economical concentrate. The key difference is that the RTU format has been specifically optimized for use in IHC and IF applications. The RTU format has a neutral pH suitable for tissue and cell-based assays and has been more highly refined to avoid the presence of flocculent material that may cause interference with microscopy work. The 5x concentrated format is best suited for membrane blotting applications.

Technical Information

This plant protein derived product is a universal antibody diluent and blocking reagent intended for cell and tissue based immunohistochemistry (IHC) and immunofluorescent (IF) applications. This ready-to-use solution can be used as an alternative to normal sera, BSA, casein and non-fat dry milk in methods where these or other blocking agents have been found to be ineffective.

It is supplied without any sodium azide, and as such, can be used with both peroxidase and alkaline phosphatase antibody conjugates and all secondary detection reagents including polymer systems and avidin/biotin reagents that incorporate these enzymes. 

Importantly, this is a different formulation than our concentrated (5x) animal free blocker, due to the optimized conditions and neutral pH of the solution for IHC and IF methods. 

The post Animal-Free Blocker® and Diluent, R.T.U. appeared first on VectorLabs.

]]> VECTASTAIN® Elite® ABC Universal PLUS Kit, Peroxidase (Horse Anti-Mouse/Rabbit IgG) https://staging.vectorlabs.com/products/vectastain-elite-hrp-horse-anti-mouse-rabbit-igg/ Tue, 02 May 2023 20:43:16 +0000 https://staging.vectorlabs.com/?post_type=product&p=14741 Description

The VECTASTAIN Elite ABC system is the most sensitive avidin/biotin-based peroxidase system and is approximately 5 times more sensitive than the original VECTASTAIN ABC Kit with the same low background. Our Universal Biotinylated Anti-Mouse/Rabbit IgG secondary antibody included in this kit is a cocktail of biotinylated anti-mouse IgG and anti-rabbit IgG, designed for use with both rabbit and mouse primary antibodies. This antibody should not be used to stain tissues from rodents or rabbits due to its reactivity with IgG present in the tissues of these species.

Features:

  • Advanced avidin/biotin technology: The Elite ABC complex is smaller, very uniform, and highly active, allowing more accessibility for binding to a biotinylated target
  • Cost effective: Higher sensitivity leads to lower cost per slide
  • More complete detection systems: Inclusion of BLOXALL® endogenous enzyme quenching solution and ImmPACT® DAB EqV substrate in the PLUS kit provides added convenience with optimized, matched reagents in the one product.

Specifications

Unit Size 1 Kit
Applications Immunohistochemistry / Immunocytochemistry, In situ hybridization, Blotting Applications, Elispot, ELISAs
Technology ABC Elite (Avidin/Biotin) Systems
Detection Enzyme(s) Peroxidase
Target Species Universal (Mouse/Rabbit)
Format Ready-to-Use

Kit Contents:

  • 50 ml ready-to-use stabilized Elite ABC reagent
  • 50 ml ready-to-use universal, biotinylated anti-mouse/rabbit IgG
  • 50 ml 2.5% normal horse serum
  • 50 ml BLOXALL Endogenous Enzyme Blocking Solution
  • ImmPACT DAB EqV Substrate (25 ml Reagent 1 + 25 ml Reagent 2)

The post VECTASTAIN® Elite® ABC Universal PLUS Kit, Peroxidase (Horse Anti-Mouse/Rabbit IgG) appeared first on VectorLabs.

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Description

The VECTASTAIN Elite ABC system is the most sensitive avidin/biotin-based peroxidase system and is approximately 5 times more sensitive than the original VECTASTAIN ABC Kit with the same low background. Our Universal Biotinylated Anti-Mouse/Rabbit IgG secondary antibody included in this kit is a cocktail of biotinylated anti-mouse IgG and anti-rabbit IgG, designed for use with both rabbit and mouse primary antibodies. This antibody should not be used to stain tissues from rodents or rabbits due to its reactivity with IgG present in the tissues of these species.

Features:

  • Advanced avidin/biotin technology: The Elite ABC complex is smaller, very uniform, and highly active, allowing more accessibility for binding to a biotinylated target
  • Cost effective: Higher sensitivity leads to lower cost per slide
  • More complete detection systems: Inclusion of BLOXALL® endogenous enzyme quenching solution and ImmPACT® DAB EqV substrate in the PLUS kit provides added convenience with optimized, matched reagents in the one product.

Specifications

Unit Size1 Kit
ApplicationsImmunohistochemistry / Immunocytochemistry, In situ hybridization, Blotting Applications, Elispot, ELISAs
TechnologyABC Elite (Avidin/Biotin) Systems
Detection Enzyme(s)Peroxidase
Target SpeciesUniversal (Mouse/Rabbit)
FormatReady-to-Use

Kit Contents:

  • 50 ml ready-to-use stabilized Elite ABC reagent
  • 50 ml ready-to-use universal, biotinylated anti-mouse/rabbit IgG
  • 50 ml 2.5% normal horse serum
  • 50 ml BLOXALL Endogenous Enzyme Blocking Solution
  • ImmPACT DAB EqV Substrate (25 ml Reagent 1 + 25 ml Reagent 2)

Documents

Product FAQs

We offer a choice of either peroxidase or alkaline phosphatase based VECTASTAIN ABC kit detection systems. Selection of which enzyme system to use would be the first step. Most IHC applications involve detecting an unconjugated primary antibody. Consider the species in which the primary antibody is raised (e.g. rabbit), and then match to to corresponding species-specific VECTASTAIN ABC kit (e.g. VECTASTAIN ABC Rabbit IgG kit). If you already have a biotinylated target (i.e. biotinylated secondary antibody or primary antibody), then a standard VECTASTAIN ABC kit containing just the avidin/biotinylated enzyme reagents would be required. Note that only the VECTASTAIN Universal Elite PLUS ABC kit (PK-8200) contains a substrate.

Citations

Technical Information

With more than 50,000 citations to its credit, the VECTASTAIN ABC kit remains widely popular. Based on the versatile avidin-biotin complex interaction, the system is modular, and along with our selection of secondary antibodies, can accommodate a wide array of primary antibody and tissue species. Our ABC kits are economical and continue to be a staple product in any immunohistochemistry (IHC) and immunocytochemistry (ICC) laboratory.

Peroxidase substrates produce sharp, dense precipitates with crisp localization. These characteristics, in conjunction with the high sensitivity and low background of the VECTASTAIN ABC systems, make the peroxidase enzyme a preferred choice in many applications. (eg. In neural tissue, the peroxidase system is often preferred because it gives more consistent labeling of both cell bodies and processes.)

VECTASTAIN Elite ABC Kit Components:

  • 50 ml ready-to-use stabilized Elite® ABC reagent
  • 50 ml ready-to-use universal, biotinylated anti-mouse/rabbit IgG
  • 50 ml 2.5% normal horse serum
  • 50 ml BLOXALL Endogenous Enzyme Blocking Solution
  • ImmPACT DAB EqV Substrate (25 ml Reagent 1 + 25 ml Reagent 2)

Sufficient reagents to stain approximately 250-500 sections.

The Avidin Biotin Complex Method

The VECTASTAIN ABC systems are extremely sensitive due to the form and number of active enzyme molecules associated with the preformed Avidin/Biotinylated enzyme Complex. This ABC complex takes advantage of two important properties of avidin: 1) an extraordinarily high affinity for biotin (over one million times higher than antibody for most antigens), and 2) four biotin-binding sites. These properties allow macromolecular complexes (ABCs) to be formed by mixing Avidin DH (Reagent A) with its paired biotinylated enzyme (Reagent B) prior to use. The ABC reagent, once formed, remains stable for many hours after formation and can be used for several days after preparation.

Biotinylated primary antibodies, secondaries, lectins, neuronal tracers, nucleic acids, and ligands can be effectively visualized in applications such as:

  • Tissue staining
  • Multiple labeling (Multiplex IHC)
  • Western blotting
  • Southern and northern blotting
  • In situ hybridization detection (ISH)
  • Enzyme immunoassays (ELISA)
  • Neuronal tracing

All applications benefit from the high sensitivity, low background, reproducibility, and economy of the VECTASTAIN ABC system.

ABCuse 1 jpg

Choosing a VECTASTAIN ABC Kit

1) Choose an enzyme system:

The VECTASTAIN ABC Kits are available with a choice of two different enzyme systems

  • Peroxidase (ABC, Elite® ABC, RTU or Kits)
  • Alkaline Phosphatase (ABC-AP)

 

2) Choose the biotinylated secondary antibody:

To detect a biotinylated target, you will need only the ABC Reagent contained in the Standard Kit, followed by an appropriate substrate.

To detect an unlabeled primary antibody, you will need a biotinylated secondary antibody that binds to the primary antibody species chosen, the ABC Reagent, and an appropriate substrate. For example, to detect a primary antibody made in rabbit, the appropriate choice is a VECTASTAIN ABC Kit designated “Rabbit IgG”.

For additional versatility and convenience, Vector Laboratories offers secondary antibody products that recognize more than one species of primary antibody. Our Universal Biotinylated Anti-Mouse/Rabbit IgG secondary antibody (Cat. No. BA-1400) is a cocktail of biotinylated anti-mouse IgG and anti-rabbit IgG, designed for use with both rabbit and mouse primary antibodies. This antibody should not be used to stain tissues from rodents or rabbits due to its reactivity with IgG present in the tissues of these species. The Universal Pan-Specific Anti- Mouse/Rabbit/Goat secondary antibody (Cat. No. BA-1300) (which is included in our VECTASTAIN Universal Kits) is a cocktail of biotinylated anti-mouse IgG, anti-rabbit IgG, and anti-goat IgG that recognizes mouse, rabbit, and goat primary antibodies. This secondary antibody should not be used to stain rat, mouse, or other rodent, rabbit, goat, bovine, or sheep tissue due to potential reactivity with endogenous IgG.

3) Choose a convenient format:

Finally, several kits are available in prediluted, stabilized formats for convenience and ease of use. See kits designated R.T.U. or Ready-To-Use.

Consider Species Cross-Reactivity

When choosing the optimal detection system for your application, it is important to consider not only the species of the primary antibody but also the species of the tissue under examination. If the species of the primary antibody and the species of the tissue are closely related (e.g. rat and mouse), the biotinylated secondary antibody may cross react with endogenous IgG in the tissue section leading to background staining. The following three options minimize background staining in these instances:

  • Biotinylate the primary antibody and detect with a Standard VECTASTAIN Elite ABC Kit, PK-6100 (only includes the avidin-biotin complex, or ABC reagent).
  • Use a biotinylated secondary antibody specifically adsorbed to remove cross-reacting antibodies of closely-related species (e.g. biotinylated anti-mouse IgG, rat adsorbed, Cat. No. BA-2001).
  • Use the M.O.M.® Immunodetection System for applications of mouse primary antibodies on mouse tissue.

 

Substrates

After choosing the VECTASTAIN ABC Kit for your application, select a substrate that matches the enzyme system of the kit.

The post VECTASTAIN® Elite® ABC Universal PLUS Kit, Peroxidase (Horse Anti-Mouse/Rabbit IgG) appeared first on VectorLabs.

]]> ImmPRESS® HRP Horse Anti-Mouse IgG PLUS Polymer Kit, Peroxidase https://staging.vectorlabs.com/products/immpress-hrp-horse-anti-mouse-igg-plus-kit/ Tue, 02 May 2023 20:35:50 +0000 https://staging.vectorlabs.com/?post_type=product&p=14729 Description

The ImmPRESS polymerized reporter enzyme staining system uses novel conjugation and micropolymer chemistries to create a highly sensitive, ready-to-use, one-step detection system. This unique micropolymer of highly active enzyme (peroxidase) is attached to our affinity purified secondary antibodies, producing reagents with outstanding sensitivity and low background. The inclusion of BLOXALL® endogenous enzyme quenching solution and ImmPACT® DAB EqV substrate in the PLUS kit provides a more complete detection system.

Features:

  • Enhanced accessibility to nuclear and membrane antigens
  • Ready-to-use in a convenient dropper bottle
  • Shorter assay time
  • Simplified multiple labeling

 

The ImmPRESS (15 ml) Kit will stain approximately 75-150 sections.

Specifications

Unit Size 1 Kit
Applications Immunohistochemistry / Immunocytochemistry, In situ hybridization, ELISAs
Technology Micropolymer Reagents
Detection Enzyme(s) Peroxidase
Conjugate Micropolymer HRP
Format Ready-to-Use

Kit Contents:

  • 15 ml ImmPRESS (Peroxidase) Polymer Anti-Mouse IgG Reagent (made in horse, ready-to-use)
  • 15 ml 2.5% Normal Horse Serum for blocking (ready-to-use)
  • 15 ml BLOXALL Endogenous Enzyme Blocking Solution
  • ImmPACT DAB EqV Substrate (7.5 ml Reagent 1 + 7.5 ml Reagent 2)

The post ImmPRESS® HRP Horse Anti-Mouse IgG PLUS Polymer Kit, Peroxidase appeared first on VectorLabs.

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Description

The ImmPRESS polymerized reporter enzyme staining system uses novel conjugation and micropolymer chemistries to create a highly sensitive, ready-to-use, one-step detection system. This unique micropolymer of highly active enzyme (peroxidase) is attached to our affinity purified secondary antibodies, producing reagents with outstanding sensitivity and low background. The inclusion of BLOXALL® endogenous enzyme quenching solution and ImmPACT® DAB EqV substrate in the PLUS kit provides a more complete detection system.

Features:

  • Enhanced accessibility to nuclear and membrane antigens
  • Ready-to-use in a convenient dropper bottle
  • Shorter assay time
  • Simplified multiple labeling

The ImmPRESS (15 ml) Kit will stain approximately 75-150 sections.

Specifications

Unit Size1 Kit
ApplicationsImmunohistochemistry / Immunocytochemistry, In situ hybridization, ELISAs
TechnologyMicropolymer Reagents
Detection Enzyme(s)Peroxidase
ConjugateMicropolymer HRP
FormatReady-to-Use

Kit Contents:

  • 15 ml ImmPRESS (Peroxidase) Polymer Anti-Mouse IgG Reagent (made in horse, ready-to-use)
  • 15 ml 2.5% Normal Horse Serum for blocking (ready-to-use)
  • 15 ml BLOXALL Endogenous Enzyme Blocking Solution
  • ImmPACT DAB EqV Substrate (7.5 ml Reagent 1 + 7.5 ml Reagent 2)

Documents

Technical Information

The peroxidase micropolymers of the ImmPRESS HRP polymer reagent limit steric interference and provide enhanced accessibility to the target, avoiding the disadvantages of other polymer systems that use large dextrans or other macromolecules as backbones. The result is crisp, strong staining of antibody targets, especially nuclear and membrane antigens (such as Ki67, estrogen receptor, bcl-2, CD3, CD8 and CD10) and greater sensitivity than other polymer systems.

Staining Procedure

The staining procedure is simple as shown in the diagram below. Following a blocking step with the diluted normal horse serum, sections are incubated with primary antibody. After a brief wash, the appropriate ImmPRESS Reagent is added to the sections and incubated for 30 minutes. Sections are again rinsed and the slides are developed with the peroxidase substrate of choice.

ImmPRESS SL600 jpg

 

Consider Species Cross-Reactivity

When choosing the optimal detection system for your application, it is important to consider not only the species of the primary antibody but also the species of the tissue. If the species of the primary antibody and the species of the tissue are closely related (e.g. rat and mouse), the secondary antibody may bind to endogenous IgG in the tissue section leading to background. The following options minimize background staining in these instances:

  • Use a secondary antibody specifically adsorbed to remove cross-reacting antibodies of closely-related species (e.g. ImmPRESS Anti-Mouse IgG, Rat Adsorbed).
  • Use the M.O.M.® ImmPRESS Kit (Cat. No. MP-2400) for applications of mouse primary antibodies on mouse tissue.

Product review on Biocompare.com.

 

The post ImmPRESS® HRP Horse Anti-Mouse IgG PLUS Polymer Kit, Peroxidase appeared first on VectorLabs.

]]> ImmPRESS® HRP Horse Anti-Rabbit IgG PLUS Polymer Kit, Peroxidase https://staging.vectorlabs.com/products/immpress-hrp-horse-anti-rabbit-igg-plus-kit/ Tue, 02 May 2023 20:31:43 +0000 https://staging.vectorlabs.com/?post_type=product&p=14720 Description

The ImmPRESS polymerized reporter enzyme staining system uses novel conjugation and micropolymer chemistries to create a highly sensitive, ready-to-use, one-step detection system. This unique micropolymer of highly active enzyme (peroxidase or alkaline phosphatase) is attached to our affinity purified secondary antibodies, producing reagents with outstanding sensitivity and low background. The inclusion of BLOXALL® endogenous enzyme quenching solution and ImmPACT® DAB EqV substrate in the PLUS kit provides a more complete detection system.

Features:

  • Enhanced accessibility to nuclear and membrane antigens
  • Ready-to-use in a convenient dropper bottle
  • Shorter assay time
  • Simplified multiple labeling

 

The ImmPRESS (15 ml) Kit will stain approximately 75-150 sections.

Specifications

Unit Size 1 Kit
Applications Immunohistochemistry / Immunocytochemistry, In situ hybridization, ELISAs
Technology Micropolymer Reagents
Detection Enzyme(s) Peroxidase
Conjugate Micropolymer HRP
Target Species Rabbit
Format Ready-to-Use

Kit Contents:

  • 15 ml ImmPRESS (Peroxidase) Polymer Anti-Rabbit IgG Reagent (made in horse, ready-to-use)
  • 15 ml 2.5% Normal Horse Serum for blocking (ready-to-use)
  • 15 ml BLOXALL Endogenous Enzyme Blocking Solution
  • ImmPACT DAB EqV Substrate (7.5 ml Reagent 1 + 7.5 ml Reagent 2)

The post ImmPRESS® HRP Horse Anti-Rabbit IgG PLUS Polymer Kit, Peroxidase appeared first on VectorLabs.

]]>

Description

The ImmPRESS polymerized reporter enzyme staining system uses novel conjugation and micropolymer chemistries to create a highly sensitive, ready-to-use, one-step detection system. This unique micropolymer of highly active enzyme (peroxidase or alkaline phosphatase) is attached to our affinity purified secondary antibodies, producing reagents with outstanding sensitivity and low background. The inclusion of BLOXALL® endogenous enzyme quenching solution and ImmPACT® DAB EqV substrate in the PLUS kit provides a more complete detection system.

Features:

  • Enhanced accessibility to nuclear and membrane antigens
  • Ready-to-use in a convenient dropper bottle
  • Shorter assay time
  • Simplified multiple labeling

The ImmPRESS (15 ml) Kit will stain approximately 75-150 sections.

Specifications

Unit Size1 Kit
ApplicationsImmunohistochemistry / Immunocytochemistry, In situ hybridization, ELISAs
TechnologyMicropolymer Reagents
Detection Enzyme(s)Peroxidase
ConjugateMicropolymer HRP
Target SpeciesRabbit
FormatReady-to-Use

Kit Contents:

  • 15 ml ImmPRESS (Peroxidase) Polymer Anti-Rabbit IgG Reagent (made in horse, ready-to-use)
  • 15 ml 2.5% Normal Horse Serum for blocking (ready-to-use)
  • 15 ml BLOXALL Endogenous Enzyme Blocking Solution
  • ImmPACT DAB EqV Substrate (7.5 ml Reagent 1 + 7.5 ml Reagent 2)

Documents

Technical Information

The peroxidase micropolymers of the ImmPRESS HRP polymer reagent limit steric interference and provide enhanced accessibility to the target, avoiding the disadvantages of other polymer systems that use large dextrans or other macromolecules as backbones. The result is crisp, strong staining of antibody targets, especially nuclear and membrane antigens (such as Ki67, estrogen receptor, bcl-2, CD3, CD8 and CD10) and greater sensitivity than other polymer systems.

Staining Procedure

The staining procedure is simple as shown in the diagram below. Following a blocking step with the diluted normal horse serum, sections are incubated with primary antibody. After a brief wash, the appropriate ImmPRESS Reagent is added to the sections and incubated for 30 minutes. Sections are again rinsed and the slides are developed with the peroxidase substrate of choice.

ImmPRESS SL600 jpg

 

Consider Species Cross-Reactivity

When choosing the optimal detection system for your application, it is important to consider not only the species of the primary antibody but also the species of the tissue. If the species of the primary antibody and the species of the tissue are closely related (e.g. rat and mouse), the secondary antibody may bind to endogenous IgG in the tissue section leading to background. The following options minimize background staining in these instances:

  • Use a secondary antibody specifically adsorbed to remove cross-reacting antibodies of closely-related species (e.g. ImmPRESS Anti-Mouse IgG, Rat Adsorbed).
  • Use the M.O.M.® ImmPRESS Kit (Cat. No. MP-2400) for applications of mouse primary antibodies on mouse tissue.

Product review on Biocompare.com.

 

The post ImmPRESS® HRP Horse Anti-Rabbit IgG PLUS Polymer Kit, Peroxidase appeared first on VectorLabs.

]]> ImmPRESS® HRP Universal (Horse Anti-Mouse/Rabbit IgG) PLUS Polymer Kit, Peroxidase https://staging.vectorlabs.com/products/immpress-hrp-plus-horse-anti-mouse-rabbit-igg/ Tue, 02 May 2023 20:25:30 +0000 https://staging.vectorlabs.com/?post_type=product&p=14712 Description

The ImmPRESS polymerized reporter enzyme staining system uses novel conjugation and micropolymer chemistries to create a highly sensitive, ready-to-use, one-step detection system. The Inclusion of BLOXALL® endogenous enzyme quenching solution and ImmPACT® DAB EqV substrate in the PLUS kit provides added convenience with optimized, matched reagents in one product.

Features:

  • High sensitivity and low background
  • Enhanced accessibility to nuclear and membrane antigens
  • Ready-to-use in a convenient dropper bottle
  • Shorter assay time
  • Simplified multiple labeling
  • Designed for use with both rabbit and mouse primary antibodies

The ImmPRESS (15 ml) Kit will stain approximately 75-150 sections.

Note: This polymer detection kit should not be used to stain tissues from rodents or rabbits due to its reactivity with IgG present in the tissues of these species.

Specifications

Unit Size 1 Kit
Applications Immunohistochemistry / Immunocytochemistry, In situ hybridization, ELISAs
Technology Micropolymer Reagents
Detection Enzyme(s) Peroxidase
Conjugate Micropolymer HRP
Target Species Universal (Mouse/Rabbit)
Format Ready-to-Use

Kit Contents:

  • 15 ml ImmPRESS Universal Antibody (anti-mouse IgG/anti-rabbit IgG, Peroxidase) Polymer Reagent (made in horse, ready-to-use)
  • 15 ml 2.5% Normal Horse Serum for blocking (ready-to-use)
  • 15 ml BLOXALL Endogenous Enzyme Blocking Solution
  • ImmPACT DAB EqV Substrate (7.5 ml Reagent 1 + 7.5 ml Reagent 2)

The post ImmPRESS® HRP Universal (Horse Anti-Mouse/Rabbit IgG) PLUS Polymer Kit, Peroxidase appeared first on VectorLabs.

]]>

Description

 

The ImmPRESS polymerized reporter enzyme staining system uses novel conjugation and micropolymer chemistries to create a highly sensitive, ready-to-use, one-step detection system. The Inclusion of BLOXALL® endogenous enzyme quenching solution and ImmPACT® DAB EqV substrate in the PLUS kit provides added convenience with optimized, matched reagents in one product. 

Features:

  • High sensitivity and low background
  • Enhanced accessibility to nuclear and membrane antigens
  • Ready-to-use in a convenient dropper bottle
  • Shorter assay time
  • Simplified multiple labeling
  • Designed for use with both rabbit and mouse primary antibodies

 

The ImmPRESS (15 ml) Kit will stain approximately 75-150 sections.

Note: This polymer detection kit should not be used to stain tissues from rodents or rabbits due to its reactivity with IgG present in the tissues of these species.

Specifications

Unit Size1 Kit
ApplicationsImmunohistochemistry / Immunocytochemistry, In situ hybridization, ELISAs
TechnologyMicropolymer Reagents
Detection Enzyme(s)Peroxidase
ConjugateMicropolymer HRP
Target SpeciesUniversal (Mouse/Rabbit)
FormatReady-to-Use

Kit Contents:

  • 15 ml ImmPRESS Universal Antibody (anti-mouse IgG/anti-rabbit IgG, Peroxidase) Polymer Reagent (made in horse, ready-to-use)
  • 15 ml 2.5% Normal Horse Serum for blocking (ready-to-use)
  • 15 ml BLOXALL Endogenous Enzyme Blocking Solution
  • ImmPACT DAB EqV Substrate (7.5 ml Reagent 1 + 7.5 ml Reagent 2)

Documents

Technical Information

The peroxidase micropolymers of the ImmPRESS HRP polymer reagent limit steric interference and provide enhanced accessibility to the target, avoiding the disadvantages of other polymer systems that use large dextrans or other macromolecules as backbones. The result is crisp, strong staining of antibody targets, especially nuclear and membrane antigens (such as Ki67, estrogen receptor, bcl-2, CD3, CD8 and CD10) and greater sensitivity than other polymer systems.

Staining Procedure

The staining procedure is simple as shown in the diagram below. Following a blocking step with the diluted normal horse serum, sections are incubated with primary antibody. After a brief wash, the appropriate ImmPRESS Reagent is added to the sections and incubated for 30 minutes. Sections are again rinsed and the slides are developed with the peroxidase substrate of choice.

ImmPRESS SL600 jpg

 
 

Consider Species Cross-Reactivity

When choosing the optimal detection system for your application, it is important to consider not only the species of the primary antibody but also the species of the tissue. If the species of the primary antibody and the species of the tissue are closely related (e.g. rat and mouse), the secondary antibody may bind to endogenous IgG in the tissue section leading to background. The following options minimize background staining in these instances:

  • Use a secondary antibody specifically adsorbed to remove cross-reacting antibodies of closely-related species (e.g. ImmPRESS Anti-Mouse IgG, Rat Adsorbed).
  • Use the M.O.M.® ImmPRESS Kit (Cat. No. MP-2400) for applications of mouse primary antibodies on mouse tissue.

Product review on Biocompare.com.

 

The post ImmPRESS® HRP Universal (Horse Anti-Mouse/Rabbit IgG) PLUS Polymer Kit, Peroxidase appeared first on VectorLabs.

]]> ImmPRESS® Duet Double Staining Polymer Kit (HRP Anti-Mouse IgG-brown, AP Anti-Rabbit IgG-magenta) https://staging.vectorlabs.com/products/immpress-duet-double-staining-kit-red/ Tue, 02 May 2023 20:20:29 +0000 https://staging.vectorlabs.com/?post_type=product&p=14695 Description

This ImmPRESS Duet Double Staining Polymer Kit enables fast, well-defined localization and visualization of two different target antigens on the same tissue section. Utilizing our unique ImmPRESS micropolymer chemistry, we independently conjugate highly-active horseradish peroxidase (HRP) and alkaline phosphatase (AP) enzyme polymers to anti-mouse IgG and anti-rabbit IgG affinity-purified, highly cross-adsorbed secondary antibodies.

Features:

Save time and streamline your double staining IHC workflow with the ImmPRESS Duet Double Staining Polymer Kit.

  • Complete Kits for IHC Double Labeling
  • Convenient Dual Specificity (anti-mouse IgG/rabbit IgG) ImmPRESS HRP/AP Polymer Reagent
  • High sensitivity and Specificity
  • Non-Biotin Based Reagents
  • Prediluted, Ready-to-use

Kit Contents:

  • 15 ml BLOXALL® Blocking Solution
  • 15 ml 2.5% Normal Serum
  • 15 ml ImmPRESS Duet HRP/AP Polymer Reagent (mixture of ImmPRESS HRP Anti-Mouse IgG and ImmPRESS AP Anti-Rabbit IgG)
  • ImmPACT® DAB EqV Substrate (HRP, brown)
  • ImmPACT Vector® Red Substrate (AP, magenta)

Note: The ImmPRESS Duet Double Staining Polymer Kit is intended for use on non-rodent tissue specimens.

Specifications

Unit Size 1 kit
Applications Immunohistochemistry / Immunocytochemistry
Technology Micropolymer Reagents
Detection Enzyme(s) HRP/AP
Conjugate Micropolymer HRP/Micropolymer AP
Target Species Universal (Mouse/Rabbit)
Format Ready-to-Use
Blocking Action Endogenous Peroxidase, Endogenous Alkaline Phosphatase, Non-Specific Protein Blocking

 

The post ImmPRESS® Duet Double Staining Polymer Kit (HRP Anti-Mouse IgG-brown, AP Anti-Rabbit IgG-magenta) appeared first on VectorLabs.

]]>

Description

 

This ImmPRESS Duet Double Staining Polymer Kit enables fast, well-defined localization and visualization of two different target antigens on the same tissue section. Utilizing our unique ImmPRESS micropolymer chemistry, we independently conjugate highly-active horseradish peroxidase (HRP) and alkaline phosphatase (AP) enzyme polymers to anti-mouse IgG and anti-rabbit IgG affinity-purified, highly cross-adsorbed secondary antibodies.

Features:

Save time and streamline your double staining IHC workflow with the ImmPRESS Duet Double Staining Polymer Kit.

  • Complete Kits for IHC Double Labeling
  • Convenient Dual Specificity (anti-mouse IgG/rabbit IgG) ImmPRESS HRP/AP Polymer Reagent
  • High sensitivity and Specificity
  • Non-Biotin Based Reagents
  • Prediluted, Ready-to-use

 

Kit Contents:

  • 15 ml BLOXALL® Blocking Solution
  • 15 ml 2.5% Normal Serum
  • 15 ml ImmPRESS Duet HRP/AP Polymer Reagent (mixture of ImmPRESS HRP Anti-Mouse IgG and ImmPRESS AP Anti-Rabbit IgG)
  • ImmPACT® DAB EqV Substrate (HRP, brown)
  • ImmPACT Vector® Red Substrate (AP, magenta)

 

Note: The ImmPRESS Duet Double Staining Polymer Kit is intended for use on non-rodent tissue specimens.

Specifications

Unit Size1 kit
ApplicationsImmunohistochemistry / Immunocytochemistry
TechnologyMicropolymer Reagents
Detection Enzyme(s)HRP/AP
ConjugateMicropolymer HRP/Micropolymer AP
Target SpeciesUniversal (Mouse/Rabbit)
FormatReady-to-Use
Blocking ActionEndogenous Peroxidase, Endogenous Alkaline Phosphatase, Non-Specific Protein Blocking

Documents

 

Technical Information

Save time and streamline your double staining IHC workflow with the ImmPRESS Duet Double Staining Polymer Kit. These reagents are blended at optimized dilutions to provide a stable pre-diluted, ready-to-use HRP/AP formula that will detect mouse and rabbit primary antibodies, and facilitate a time-saving, simplified, one-step double label detection protocol. For maximum sensitivity and staining contrast between target antigens, ImmPACT DAB EqV (HRP, brown) and ImmPACT Vector Red (AP, magenta) substrates are included to complete this easy to use staining kit.

Reliable and reproducible results are obtained on tissue sections where the two target antigens do not over-lap (co-localize) in the same structure of the same cell, but rather are expressed in different cell compartments or different cell types.

Also included, BLOXALL Blocking Solution abolishes all endogenous peroxidase and alkaline phosphatase activity in a single 10 minute incubation. In addition, 2.5% Normal Serum blocks all non-specific binding of detection reagents to ensure crisp, specific staining.

All reagents are supplied in stabilized, ready-to-use (R.T.U.) solutions for maximum convenience, and consistency between assays. The ImmPRESS Duet Double Staining Polymer Kits offer a complete and simple solution for visualizing target antigens in cell and tissue preparations and contains sufficient reagents to stain approximately 150 sections.

Product review on Biocompare.com.

The post ImmPRESS® Duet Double Staining Polymer Kit (HRP Anti-Mouse IgG-brown, AP Anti-Rabbit IgG-magenta) appeared first on VectorLabs.

]]> ImmPRESS® Duet Double Staining Polymer Kit (HRP – Anti-Rabbit IgG-brown, AP – Anti-Mouse IgG-magenta) https://staging.vectorlabs.com/products/immpress-duet-staining-kit-rb-br-ms-red/ Tue, 02 May 2023 20:12:52 +0000 https://staging.vectorlabs.com/?post_type=product&p=14675 Description

This ImmPRESS Duet Double Staining Polymer Kit enables fast, well-defined localization and visualization of two different target antigens on the same tissue section. Utilizing our unique ImmPRESS micropolymer chemistry, we independently conjugate highly-active horseradish peroxidase (HRP) and alkaline phosphatase (AP) enzyme polymers to anti-rabbit IgG and anti-mouse IgG affinity-purified, highly cross-adsorbed secondary antibodies.

Features:

Save time and streamline your double staining IHC workflow with the ImmPRESS Duet Double Staining Polymer Kit.

  • Complete Kits for IHC Double Labeling
  • Convenient Dual Specificity (anti-mouse IgG/rabbit IgG) ImmPRESS HRP/AP Polymer Reagent
  • High Sensitivity and Specificity
  • Non-Biotin Based Reagents
  • Prediluted, Ready-to-use

Kit Contents:

  • 15 ml BLOXALL® Blocking Solution
  • 15 ml 2.5% Normal Serum
  • 15 ml ImmPRESS Duet HRP/AP Polymer Reagent (mixture of ImmPRESS HRP Anti-Rabbit IgG and ImmPRESS  AP Anti-Mouse IgG)
  • ImmPACT® DAB EqV Substrate (HRP, brown)
  • ImmPACT Vector® Red Substrate (AP, magenta)

 

Note: The ImmPRESS Duet Double Staining Polymer Kit is intended for use on non-rodent tissue specimens.

Specifications

Unit Size 1 kit
Applications Immunohistochemistry / Immunocytochemistry
Technology Micropolymer Reagents
Detection Enzyme(s) HRP/AP
Conjugate Micropolymer HRP/Micropolymer AP
Target Species Universal (Mouse/Rabbit)
Format Ready-to-Use
Blocking Action Endogenous Peroxidase, Endogenous Alkaline Phosphatase, Non-Specific Protein Blocking

The post ImmPRESS® Duet Double Staining Polymer Kit (HRP – Anti-Rabbit IgG-brown, AP – Anti-Mouse IgG-magenta) appeared first on VectorLabs.

]]>

Description

 

This ImmPRESS Duet Double Staining Polymer Kit enables fast, well-defined localization and visualization of two different target antigens on the same tissue section. Utilizing our unique ImmPRESS micropolymer chemistry, we independently conjugate highly-active horseradish peroxidase (HRP) and alkaline phosphatase (AP) enzyme polymers to anti-rabbit IgG and anti-mouse IgG affinity-purified, highly cross-adsorbed secondary antibodies.

Features:

Save time and streamline your double staining IHC workflow with the ImmPRESS Duet Double Staining Polymer Kit.

  • Complete Kits for IHC Double Labeling
  • Convenient Dual Specificity (anti-mouse IgG/rabbit IgG) ImmPRESS HRP/AP Polymer Reagent
  • High Sensitivity and Specificity
  • Non-Biotin Based Reagents
  • Prediluted, Ready-to-use

 

Kit Contents:

  • 15 ml BLOXALL® Blocking Solution
  • 15 ml 2.5% Normal Serum
  • 15 ml ImmPRESS Duet HRP/AP Polymer Reagent (mixture of ImmPRESS HRP Anti-Rabbit IgG and ImmPRESS  AP Anti-Mouse IgG)
  • ImmPACT® DAB EqV Substrate (HRP, brown)
  • ImmPACT Vector® Red Substrate (AP, magenta)

Note: The ImmPRESS Duet Double Staining Polymer Kit is intended for use on non-rodent tissue specimens.

Specifications

Unit Size1 kit
ApplicationsImmunohistochemistry / Immunocytochemistry
TechnologyMicropolymer Reagents
Detection Enzyme(s)HRP/AP
ConjugateMicropolymer HRP/Micropolymer AP
Target SpeciesUniversal (Mouse/Rabbit)
FormatReady-to-Use
Blocking ActionEndogenous Peroxidase, Endogenous Alkaline Phosphatase, Non-Specific Protein Blocking

Documents

Technical Information

Save time and streamline your double staining IHC workflow with the ImmPRESS Duet Double Staining Polymer Kit.  These reagents are blended at optimized dilutions to provide a stable pre-diluted, ready-to-use HRP/AP formula that will detect mouse and rabbit primary antibodies, and facilitate a time-saving, simplified, one-step double label detection protocol. For maximum sensitivity and staining contrast between target antigens, ImmPACT DAB EqV (HRP, brown) and ImmPACT Vector® Red (AP, magenta) substrates are included to complete this easy to use staining kit.

Reliable and reproducible results are obtained on tissue sections where the two target antigens do not over-lap (co-localize) in the same structure of the same cell, but rather are expressed in different cell compartments or different cell types.

Also included, BLOXALL  Blocking Solution abolishes all endogenous peroxidase and alkaline phosphatase activity in a single 10 minute incubation. In addition, 2.5% Normal Serum blocks all non-specific binding of detection reagents to ensure crisp, specific staining.

All reagents are supplied in stabilized, ready-to-use (R.T.U.) solutions for maximum convenience, and consistency between assays. The ImmPRESS Duet Double Staining Polymer Kits offer a complete and simple solution for visualizing target antigens in cell and tissue preparations and contains sufficient reagents to stain approximately 150 sections.

Product review on Biocompare.com.

The post ImmPRESS® Duet Double Staining Polymer Kit (HRP – Anti-Rabbit IgG-brown, AP – Anti-Mouse IgG-magenta) appeared first on VectorLabs.

]]> ImmPRESS®-AP Goat Anti-Rat IgG (Mouse Adsorbed) Polymer Detection Kit, Alkaline Phosphatase https://staging.vectorlabs.com/products/immpress-ap-anti-rat-igg-mouseads-kit/ Tue, 02 May 2023 18:53:08 +0000 https://staging.vectorlabs.com/?post_type=product&p=14491 Description

The ImmPRESS polymerized reporter enzyme staining system uses novel conjugation and micropolymer chemistries to create a highly sensitive, ready-to-use, one-step detection system. This unique micropolymer of highly active enzyme is attached to our affinity purified secondary antibodies, producing reagents with outstanding sensitivity and low background. This secondary antibody can be used to stain mouse tissue because it is specifically adsorbed to remove cross-reacting antibodies of closely-related species.

Features:

  • Non-biotin
  • Enhanced accessibility to nuclear and membrane antigens
  • Ready-to-use in a convenient dropper bottle
  • Shorter assay time
  • Simplified multiple labeling

 

The ImmPRESS (15 ml) Kit will stain approximately 75-150 sections.

Specifications

Unit Size 15 ml
Applications Immunohistochemistry / Immunocytochemistry, In situ hybridization, ELISAs
Technology Micropolymer Reagents
Detection Enzyme(s) Alkaline Phosphatase
Conjugate Micropolymer AP
Target Species Rat
Format Ready-to-Use

Kit Contents:

  • 15 ml ImmPRESS-AP (alkaline phosphatase) Polymer Anti-Rat IgG Reagent (ready-to-use)
  • 15 ml 2.5% Normal Goat Serum for blocking (ready-to-use)

The post ImmPRESS®-AP Goat Anti-Rat IgG (Mouse Adsorbed) Polymer Detection Kit, Alkaline Phosphatase appeared first on VectorLabs.

]]>

Description

The ImmPRESS polymerized reporter enzyme staining system uses novel conjugation and micropolymer chemistries to create a highly sensitive, ready-to-use, one-step detection system. This unique micropolymer of highly active enzyme is attached to our affinity purified secondary antibodies, producing reagents with outstanding sensitivity and low background. This secondary antibody can be used to stain mouse tissue because it is specifically adsorbed to remove cross-reacting antibodies of closely-related species.

Features:

  • Non-biotin
  • Enhanced accessibility to nuclear and membrane antigens
  • Ready-to-use in a convenient dropper bottle
  • Shorter assay time
  • Simplified multiple labeling

The ImmPRESS (15 ml) Kit will stain approximately 75-150 sections.

Specifications

Unit Size15 ml
ApplicationsImmunohistochemistry / Immunocytochemistry, In situ hybridization, ELISAs
TechnologyMicropolymer Reagents
Detection Enzyme(s)Alkaline Phosphatase
ConjugateMicropolymer AP
Target SpeciesRat
FormatReady-to-Use

Kit Contents:

  • 15 ml ImmPRESS-AP (alkaline phosphatase) Polymer Anti-Rat IgG Reagent (ready-to-use)
  • 15 ml 2.5% Normal Goat Serum for blocking (ready-to-use)

Documents

Product FAQs

The ImmPRESS Excel kits are a two-step peroxidase polymer based detection system that include an unconjugated amplifier antibody. This intermediate amplifier antibody increases sensitivity of the assay at least three- to four-fold over that of a one-step polymer based system, by facilitating the introduction of more peroxidase enzyme at the site of specific antigen localization. This increase in sensitivity would be advantageous in instances of weak antigen expression, and to further dilute out an expensive primary antibody. The ImmPRESS Excel Amplifier kits are presented as a complete kit format that include enzyme quench, blocking serum and ImmPACT® DAB EqV peroxidase substrate, in addition to the amplifier antibody and defined ImmPRESS polymer secondary antibody. However, as the two published references below indicate, this format is still modular and allows for the substitution of different detection reagents. These references describe using ImmPACT NovaRED™ peroxidase substrate in combination with the ImmPRESS Excel Amplifier kits. Oncogenesis (2017) 6, e293; doi:10.1038/oncsis.2016.82 Tan, E.M.S., et al (2017) Front. Med. 4:162 (doi: 10.3389/fmed.2017.00162)

Yes, we conducted studies on the compatibility of the ImmPRESS HRP polymer reagents with three commercially available autostainers (Agilent/Dako Autostainer Plus, Leica Bond Rx, and Ventana Discovery Ultra). We showed that our reagents are suitable for IHC detection on each of these platforms. The ImmPRESS polymer reagents and enzyme substrates generated equivalent IHC staining results compared to reagents from the instrument manufacturer. Some modifications in the protocol were performed to optimize the signal-to-noise ratio, such as a shorter incubation time for the ImmPRESS polymer and increase in the number of buffer washes following polymer incubation. Please see the following application note for more details about each automated platform:https://staging.vectorlabs.com/wp-content/uploads/2022/11/VL_LIT3023_ImmPRESS_AppNote_4.pdf

Technical Information

The ImmPRESS-AP Alkaline Phosphatase Polymer Reagent micropolymer technology limits steric interference and provides enhanced accessibility to the target avoiding the disadvantages of other polymer systems that use large dextrans or other macromolecules as backbones.  The result is crisp, strong staining of antibody targets, especially nuclear and membrane antigens, and  greater sensitivity than conventional alkaline phosphatase antibody conjugates and other alkaline phosphatase polymer systems for immunohistochemistry and other applications. 
 

Immunostaining Protocol

The staining procedure is simple as shown in the diagram below. Following a blocking step with the diluted normal horse serum, sections are incubated with primary antibody. After a brief wash, the appropriate ImmPRESS Reagent is added to the sections and incubated for 30 minutes. Sections are again rinsed and the slides are developed with the peroxidase substrate of choice.

ImmPRESS SL600 jpg

 

Consider Species Cross-Reactivity

When choosing the optimal detection system for your application, it is important to consider not only the species of the primary antibody but also the species of the tissue. If the species of the primary antibody and the species of the tissue are closely related (e.g. rat and mouse), the secondary antibody may bind to endogenous IgG in the tissue section leading to background. The following options minimize background staining in these instances:

  • Use a secondary antibody specifically adsorbed to remove cross-reacting antibodies of closely-related species (e.g. ImmPRESS Anti-Mouse IgG, Rat Adsorbed).
  • Use the M.O.M.® ImmPRESS Kit (Cat. No. MP-2400) for applications of mouse primary antibodies on mouse tissue.

The post ImmPRESS®-AP Goat Anti-Rat IgG (Mouse Adsorbed) Polymer Detection Kit, Alkaline Phosphatase appeared first on VectorLabs.

]]> ImmPRESS®-AP Horse Anti-Goat IgG Polymer Detection Kit, Alkaline Phosphatase https://staging.vectorlabs.com/products/immpress-ap-anti-goat-igg-kit/ Tue, 02 May 2023 18:46:28 +0000 https://staging.vectorlabs.com/?post_type=product&p=14480 Description

The ImmPRESS polymerized reporter enzyme staining system uses novel conjugation and micropolymer chemistries to create a highly sensitive, ready-to-use, one-step detection system. This unique micropolymer of highly active enzyme (alkaline phosphatase) is attached to our affinity purified secondary antibodies, producing reagents with outstanding sensitivity and low background.

Features:

  • Enhanced accessibility to nuclear and membrane antigens
  • Ready-to-use in a convenient dropper bottle
  • Shorter assay time
  • Simplified multiple labeling

Kit Contents:

  • ImmPRESS-AP (alkaline phosphatase) Polymer Anti-Goat IgG Reagent (ready-to-use)
  • 2.5% Normal Horse Serum for blocking (ready-to-use)

 

The ImmPRESS (15 ml) Kit will stain approximately 75-150 sections and the ImmPRESS (50 ml) Kit will stain approximately 250-500 sections.

Specifications

Unit Size 15 ml, 50 ml
Applications Immunohistochemistry / Immunocytochemistry, In situ hybridization, ELISAs
Technology Micropolymer Reagents
Detection Enzyme(s) Alkaline Phosphatase
Conjugate Micropolymer AP
Target Species Goat
Format Ready-to-Use

The post ImmPRESS®-AP Horse Anti-Goat IgG Polymer Detection Kit, Alkaline Phosphatase appeared first on VectorLabs.

]]>

Description

The ImmPRESS polymerized reporter enzyme staining system uses novel conjugation and micropolymer chemistries to create a highly sensitive, ready-to-use, one-step detection system. This unique micropolymer of highly active enzyme (alkaline phosphatase) is attached to our affinity purified secondary antibodies, producing reagents with outstanding sensitivity and low background.  

Features:

  • Enhanced accessibility to nuclear and membrane antigens
  • Ready-to-use in a convenient dropper bottle
  • Shorter assay time
  • Simplified multiple labeling

 

Kit Contents:

  • ImmPRESS-AP (alkaline phosphatase) Polymer Anti-Goat IgG Reagent (ready-to-use)
  • 2.5% Normal Horse Serum for blocking (ready-to-use)

The ImmPRESS (15 ml) Kit will stain approximately 75-150 sections and the ImmPRESS (50 ml) Kit will stain approximately 250-500 sections.

Specifications

Unit Size15 ml, 50 ml
ApplicationsImmunohistochemistry / Immunocytochemistry, In situ hybridization, ELISAs
TechnologyMicropolymer Reagents
Detection Enzyme(s)Alkaline Phosphatase
ConjugateMicropolymer AP
Target SpeciesGoat
FormatReady-to-Use

Documents

Product FAQs

The ImmPRESS Excel kits are a two-step peroxidase polymer based detection system that include an unconjugated amplifier antibody. This intermediate amplifier antibody increases sensitivity of the assay at least three- to four-fold over that of a one-step polymer based system, by facilitating the introduction of more peroxidase enzyme at the site of specific antigen localization. This increase in sensitivity would be advantageous in instances of weak antigen expression, and to further dilute out an expensive primary antibody. The ImmPRESS Excel Amplifier kits are presented as a complete kit format that include enzyme quench, blocking serum and ImmPACT® DAB EqV peroxidase substrate, in addition to the amplifier antibody and defined ImmPRESS polymer secondary antibody. However, as the two published references below indicate, this format is still modular and allows for the substitution of different detection reagents. These references describe using ImmPACT NovaRED™ peroxidase substrate in combination with the ImmPRESS Excel Amplifier kits. Oncogenesis (2017) 6, e293; doi:10.1038/oncsis.2016.82 Tan, E.M.S., et al (2017) Front. Med. 4:162 (doi: 10.3389/fmed.2017.00162)

Yes, we conducted studies on the compatibility of the ImmPRESS HRP polymer reagents with three commercially available autostainers (Agilent/Dako Autostainer Plus, Leica Bond Rx, and Ventana Discovery Ultra). We showed that our reagents are suitable for IHC detection on each of these platforms. The ImmPRESS polymer reagents and enzyme substrates generated equivalent IHC staining results compared to reagents from the instrument manufacturer. Some modifications in the protocol were performed to optimize the signal-to-noise ratio, such as a shorter incubation time for the ImmPRESS polymer and increase in the number of buffer washes following polymer incubation. Please see the following application note for more details about each automated platform:https://staging.vectorlabs.com/wp-content/uploads/2022/11/VL_LIT3023_ImmPRESS_AppNote_4.pdf

Technical Information

The ImmPRESS-AP Alkaline Phosphatase Polymer Reagent micropolymer technology limits steric interference and provides enhanced accessibility to the target avoiding the disadvantages of other polymer systems that use large dextrans or other macromolecules as backbones.  The result is crisp, strong staining of antibody targets, especially nuclear and membrane antigens, and  greater sensitivity than conventional alkaline phosphatase antibody conjugates and other alkaline phosphatase polymer systems for immunohistochemistry and other applications. 

Staining Procedure

The staining procedure is simple as shown in the diagram below. Following a blocking step with the diluted normal horse serum, sections are incubated with primary antibody. After a brief wash, the appropriate ImmPRESS Reagent is added to the sections and incubated for 30 minutes. Sections are again rinsed and the slides are developed with the peroxidase substrate of choice.

ImmPRESS SL600 jpg

 

Consider Species Cross-Reactivity

When choosing the optimal detection system for your application, it is important to consider not only the species of the primary antibody but also the species of the tissue. If the species of the primary antibody and the species of the tissue are closely related (e.g. rat and mouse), the secondary antibody may bind to endogenous IgG in the tissue section leading to background. The following options minimize background staining in these instances:

  • Use a secondary antibody specifically adsorbed to remove cross-reacting antibodies of closely-related species (e.g. ImmPRESS Anti-Mouse IgG, Rat Adsorbed).
  • Use the M.O.M.® ImmPRESS Kit (Cat. No. MP-2400) for applications of mouse primary antibodies on mouse tissue.

The post ImmPRESS®-AP Horse Anti-Goat IgG Polymer Detection Kit, Alkaline Phosphatase appeared first on VectorLabs.

]]>