DyLight 594 – VectorLabs https://staging.vectorlabs.com From linker design and synthesis, bioconjugated design and manufacturing, biomolecule labeling and functionalization, and detection system and solutions, our team is here to help you move forward, with impact. Wed, 22 Oct 2025 17:11:15 +0000 en-US hourly 1 https://wordpress.org/?v=6.9 https://staging.vectorlabs.com/wp-content/uploads/2024/11/V-favicon-02-100x100.png DyLight 594 – VectorLabs https://staging.vectorlabs.com 32 32 Ulex Europaeus Agglutinin I (UEA I), DyLight® 594 https://staging.vectorlabs.com/products/dylight-594-ulex-europaeus-agglutinin/ Tue, 02 May 2023 19:28:32 +0000 https://staging.vectorlabs.com/?post_type=product&p=14531 Description

Ulex europaeus agglutinin I binds to many glycoproteins and glycolipids containing α-linked fucose residues, such as ABO blood group glycoconjugates. This lectin preferentially binds blood group O cells and has been used to determine secretor status. It has been established as an excellent marker for human endothelial cells.

DyLight 594 labeled Ulex europaeus agglutinin I has an appropriate number of fluorochromes bound to provide the optimum staining characteristics for this lectin. This conjugate is supplied essentially free of unconjugated fluorochromes.

  • Excitation at 592 nm
  • Emission at 617 nm
  • Color:  Red

Specifications

Unit Size 1 mg
Applications Immunofluorescence, Glycobiology
Recommended Usage The recommended concentration range for use is 5-20 µg/ml. If a precipitate forms upon long-term storage, warm to 37 ºC.
Recommended Storage 2-8 °C
Maximum Excitation 592 nm
Maximum Emission 617 nm
Solution 10 mM HEPES, 0.15 M NaCl, pH 7.5, 0.08% sodium azide, 0.1 mM CaCl2.
Concentration 1 mg active conjugate/ml
Conjugate DyLight 594
Color of Fluorescence Red
Sugar Specificity Fucose, Arabinose

The post Ulex Europaeus Agglutinin I (UEA I), DyLight® 594 appeared first on VectorLabs.

]]>

Description

Ulex europaeus agglutinin I binds to many glycoproteins and glycolipids containing α-linked fucose residues, such as ABO blood group glycoconjugates. This lectin preferentially binds blood group O cells and has been used to determine secretor status. It has been established as an excellent marker for human endothelial cells.

DyLight 594 labeled Ulex europaeus agglutinin I has an appropriate number of fluorochromes bound to provide the optimum staining characteristics for this lectin. This conjugate is supplied essentially free of unconjugated fluorochromes.

  • Excitation at 592 nm
  • Emission at 617 nm
  • Color:  Red

Specifications

Unit Size1 mg
ApplicationsImmunofluorescence, Glycobiology
Recommended UsageThe recommended concentration range for use is 5-20 µg/ml. If a precipitate forms upon long-term storage, warm to 37 ºC.
Recommended Storage2-8 °C
Maximum Excitation592 nm
Maximum Emission617 nm
Solution10 mM HEPES, 0.15 M NaCl, pH 7.5, 0.08% sodium azide, 0.1 mM CaCl2.
Concentration1 mg active conjugate/ml
ConjugateDyLight 594
Color of FluorescenceRed
Sugar SpecificityFucose, Arabinose

Documents

Citations

Technical Information

Accompanying each fluorescent lectin is an analysis data sheet summarizing the results of our quality control tests and providing pertinent information on the product. All of these reagents are supplied as solutions preserved with sodium azide.

Inhibiting/Eluting Sugar: 50 mM – 100 mM L-fucose

The post Ulex Europaeus Agglutinin I (UEA I), DyLight® 594 appeared first on VectorLabs.

]]> Streptavidin, DyLight™ 594 https://staging.vectorlabs.com/products/dylight-594-streptavidin/ Mon, 01 May 2023 19:42:16 +0000 https://staging.vectorlabs.com/?post_type=product&p=13589 Description

Features:

  • Can be used to detect biotinylated secondary antibodies and other macromolecules in applications such as immunofluorescence, in situ hybridization, or flow cytometry
  • Highly purified and possess very low non-specific binding properties
  • Extremely high affinity for biotin
  • Advantages of DyLight dyes include greater photostability, pH independence, and brighter fluorescence
  • Excitation at 592 nm
  • Emission at 617 nm
  • Color:  Red

Specifications

Unit Size 1 mg
Applications Immunofluorescence, In situ hybridization, Flow Cytometry/Cell Separation
Recommended Usage Recommended concentration range for use: 5-30 µg/ml. Avoid using RPMI 1640 or other biotin-containing solutions as diluents. Serum also can contain biotin and should not be added to diluents.
Solution 10 mM HEPES, 0.15 M NaCl, pH 7.5, 0.08% sodium azide.
Maximum Excitation 592 nm
Maximum Emission 617 nm
Recommended Storage 2-8 °C
Concentration 1.0 mg/ml active conjugate
Conjugate DyLight 594
Color of Fluorescence Red
Format Concentrate

The post Streptavidin, DyLight™ 594 appeared first on VectorLabs.

]]>

Description

Features:

  • Can be used to detect biotinylated secondary antibodies and other macromolecules in applications such as immunofluorescence, in situ hybridization, or flow cytometry
  • Highly purified and possess very low non-specific binding properties
  • Extremely high affinity for biotin
  • Advantages of DyLight dyes include greater photostability, pH independence, and brighter fluorescence
  • Excitation at 592 nm
  • Emission at 617 nm
  • Color:  Red

Specifications

Unit Size1 mg
ApplicationsImmunofluorescence, In situ hybridization, Flow Cytometry/Cell Separation
Recommended UsageRecommended concentration range for use: 5-30 µg/ml. Avoid using RPMI 1640 or other biotin-containing solutions as diluents. Serum also can contain biotin and should not be added to diluents.
Solution10 mM HEPES, 0.15 M NaCl, pH 7.5, 0.08% sodium azide.
Maximum Excitation592 nm
Maximum Emission617 nm
Recommended Storage2-8 °C
Concentration1.0 mg/ml active conjugate
ConjugateDyLight 594
Color of FluorescenceRed
FormatConcentrate

Documents

Citations

Technical Information

Vector Laboratories fluorochrome-conjugated streptavidin and avidin reagents are highly purified and possess very low non-specific binding properties. They have extremely high affinity for biotin. These fluorescent conjugates can be used to detect biotinylated secondary antibodies and other macromolecules in applications such as immunofluorescence, in situ hybridization, or flow cytometry.

Amplification of fluorescent signals can be easily achieved with our biotinylated secondary antibodies followed by our highly purified fluorochrome-labeled streptavidin or avidin. Using a biotin/avidin or biotin/streptavidin detection system results in an additional layer of amplification over a directly conjugated secondary antibody.

DyLight dyes offer several advantages including greater photostability, pH independence, and brighter fluorescence. We offer DyLight conjugated streptavidin for use in a variety of applications, in particular, cell- and tissue-based immunofluorescent detection. The DyLight conjugates are completely stable from pH 4 to pH 9, making them compatible with many aqueous-based buffers and diluents.

DyLightspectra jpg

Dylight Table1

The post Streptavidin, DyLight™ 594 appeared first on VectorLabs.

]]> Griffonia Simplicifolia Lectin I (GSL I) Isolectin B4, DyLight® 594 https://staging.vectorlabs.com/products/dylight-594-gsl-i-isolectin-b4/ Tue, 25 Apr 2023 15:42:08 +0000 https://staging.vectorlabs.com/?post_type=product&p=11778 Description

GSL I-B4 isolectin contains only the B subunits. It is a useful marker for endothelial cells from nonprimates such as mouse, rat, rabbit, and goat as well as a marker for non-peptidergic unmyelinated primary afferent neurons.  This “B”-rich lectin preferentially agglutinates blood group B cells and is specific for α-galactose residues.

DyLight™ 594 GSL I-B4 isolectin is produced by using special conjugation procedures to incorporate DyLight™ 594 into our affinity-purified lectin. This conjugate has an appropriate number of fluorochromes bound which provide the maximum fluorescence and optimum staining characteristics for this particular lectin. This reagent is supplied essentially free of unconjugated fluorochromes and inactive lectin.

Specifications

Unit Size 0.5 mg
Applications Immunofluorescence, Glycobiology
Recommended Usage The recommended concentration range for use is 5-20 µg/ml.
Recommended Storage 2-8 °C
Maximum Excitation 592 nm
Inhibiting and/or Eluting Sugar Inhibiting Sugar: 500 mM galactose (S-9003) or 100 mM raffinose.
Maximum Emission 617 nm
Solution 10 mM HEPES, 0.15 M NaCl, pH 7.5, 0.08% sodium azide, 0.1 mM CaCl2.
Concentration 1 mg active conjugate/ml
Conjugate DyLight 594
Color of Fluorescence Red
Sugar Specificity Galactose

The post Griffonia Simplicifolia Lectin I (GSL I) Isolectin B4, DyLight® 594 appeared first on VectorLabs.

]]>

Description

GSL I-B4 isolectin contains only the B subunits. It is a useful marker for endothelial cells from nonprimates such as mouse, rat, rabbit, and goat as well as a marker for non-peptidergic unmyelinated primary afferent neurons.  This “B”-rich lectin preferentially agglutinates blood group B cells and is specific for α-galactose residues. 

DyLight™ 594 GSL I-B4 isolectin is produced by using special conjugation procedures to incorporate DyLight™ 594 into our affinity-purified lectin. This conjugate has an appropriate number of fluorochromes bound which provide the maximum fluorescence and optimum staining characteristics for this particular lectin. This reagent is supplied essentially free of unconjugated fluorochromes and inactive lectin.

Specifications

Unit Size0.5 mg
ApplicationsImmunofluorescence, Glycobiology
Recommended UsageThe recommended concentration range for use is 5-20 µg/ml.
Recommended Storage2-8 °C
Maximum Excitation592 nm
Inhibiting and/or Eluting SugarInhibiting Sugar: 500 mM galactose (S-9003) or 100 mM raffinose.
Maximum Emission617 nm
Solution10 mM HEPES, 0.15 M NaCl, pH 7.5, 0.08% sodium azide, 0.1 mM CaCl2.
Concentration1 mg active conjugate/ml
ConjugateDyLight 594
Color of FluorescenceRed
Sugar SpecificityGalactose

Documents

Product FAQs

Yes, there are a number of published references describing the use of TrueVIEW Autofluorescence Quenching Kit in the scientific literature. Please refer to a partial list of these publications in the Technical Information section of the product detail page for SP-8400.

Citations

Technical Information

GSL I is a family of glycoproteins with molecular weights of approximately 114 kDa. There are two types of subunits, termed A and B, with slightly different molecular weights. These subunits combine to form tetrameric structures, resulting in five isolectins. The A -rich lectin preferentially agglutinates blood group A erythrocytes and thus appears to be specific for α-N-acetylgalactosamine residues, while the B -rich lectin preferentially agglutinates blood group B cells and is specific for α-galactose residues. Our GSL I is a mixture of the five isolectins. GSL I has been reported to bind several glycoproteins including laminin.

Accompanying each fluorescent lectin is an analysis data sheet summarizing the results of our quality control tests and providing pertinent information on the product. All of these reagents are supplied as solutions preserved with sodium azide.

  • Excitation maximum:  592 nm
  • emission maximum:  617 nm
  • Color:  Red

Inhibiting Sugar: 500 mM galactose or 100 mM raffinose

The post Griffonia Simplicifolia Lectin I (GSL I) Isolectin B4, DyLight® 594 appeared first on VectorLabs.

]]> Lycopersicon Esculentum (Tomato) Lectin (LEL, TL), DyLight® 594 https://staging.vectorlabs.com/products/dylight-594-lycopersicon-esculentum-tomato/ Tue, 25 Apr 2023 15:37:12 +0000 https://staging.vectorlabs.com/?post_type=product&p=11770 Description

Tomato lectin (from Lycopersicon esculentum) is an effective marker of blood vessels and microglial cells in rodents. Conjugation of the lectin with a fluorophore facilitates fast, one-step detection and visualization using intravascular perfusion methods or direct application to tissue sections.

DyLight™ 594 labeled tomato lectin has an appropriate number of fluorochromes bound to provide the optimum staining characteristics for this lectin. This conjugate is supplied essentially free of unconjugated fluorochromes.

  • Excitation maximum:  592 nm
  • Emission maximum:  617 nm
  • Color:  Red

Specifications

Unit Size 1 mg
Applications Immunofluorescence, Glycobiology
Recommended Usage The recommended concentration range for use is 5-20 µg/ml. If a precipitate forms upon long-term storage, warm to 37 ºC.
Recommended Storage 2-8 °C
Maximum Excitation 592 nm
Maximum Emission 617 nm
Solution 10 mM HEPES, 0.15 M NaCl, pH 7.5, 0.08% sodium azide, 0.1 mM CaCl2.
Concentration 1 mg active conjugate/ml
Conjugate DyLight 594
Color of Fluorescence Red
Sugar Specificity [GlcNAc]1-3, N-Acetylglucosamine

The post Lycopersicon Esculentum (Tomato) Lectin (LEL, TL), DyLight® 594 appeared first on VectorLabs.

]]>

Description

Tomato lectin (from Lycopersicon esculentum) is an effective marker of blood vessels and microglial cells in rodents. Conjugation of the lectin with a fluorophore facilitates fast, one-step detection and visualization using intravascular perfusion methods or direct application to tissue sections.

DyLight™ 594 labeled tomato lectin has an appropriate number of fluorochromes bound to provide the optimum staining characteristics for this lectin. This conjugate is supplied essentially free of unconjugated fluorochromes.

  • Excitation maximum:  592 nm
  • Emission maximum:  617 nm
  • Color:  Red

Specifications

Unit Size1 mg
ApplicationsImmunofluorescence, Glycobiology
Recommended UsageThe recommended concentration range for use is 5-20 µg/ml. If a precipitate forms upon long-term storage, warm to 37 ºC.
Recommended Storage2-8 °C
Maximum Excitation592 nm
Maximum Emission617 nm
Solution10 mM HEPES, 0.15 M NaCl, pH 7.5, 0.08% sodium azide, 0.1 mM CaCl2.
Concentration1 mg active conjugate/ml
ConjugateDyLight 594
Color of FluorescenceRed
Sugar Specificity[GlcNAc]1-3, N-Acetylglucosamine

Documents

Product FAQs

Yes, there are a number of published references describing the use of TrueVIEW Autofluorescence Quenching Kit in the scientific literature. Please refer to a partial list of these publications in the Technical Information section of the product detail page for SP-8400.

Citations

Technical Information

Tomato lectin is a very stable single subunit glycoprotein containing about 50 percent arabinose and galactose and may form multimeric aggregates in solution. Tomato lectin, although sharing some specificities with potato lectin, Datura lectin, and wheat germ agglutinin, has been reported to be dissimilar in many respects. LEL binds well to glycophorin and Tamm-Horsfall glycoprotein and has been used effectively to label vascular endothelium in rodents.

Texas Red® or DyLight® 594 conjugated tomato lectin provides an excellent contrast to green/yellow fluorescence such as GFP expressed in transgenic animals, or with fluorescein conjugates in standard double label studies. The tomato lectin complements our existing range of lectin reagents and should be a valuable tool in examining rodent tumor angiogenesis, tracing neovascular development in xenograft models and brain research.

Accompanying each fluorescent lectin is an analysis data sheet summarizing the results of our quality control tests and providing pertinent information on the product. All of these reagents are supplied as solutions preserved with sodium azide.

Inhibiting/Eluting Sugar: Chitin Hydrolysate

The post Lycopersicon Esculentum (Tomato) Lectin (LEL, TL), DyLight® 594 appeared first on VectorLabs.

]]> VectaFluor™ Excel Amplified Anti-Mouse IgG, DyLight® 594 Antibody Kit https://staging.vectorlabs.com/products/vectafluor-excel-dylight-594-anti-mouse-igg-kit/ Tue, 25 Apr 2023 15:21:55 +0000 https://staging.vectorlabs.com/?post_type=product&p=11753 Description

VectaFluor Excel Amplified Fluorescent Staining System offers a non-biotin amplification method for fluorescence applications. This system uses a ready-to-use (R.T.U.) Amplifier Antibody, followed by a ready-to-use (R.T.U.) VectaFluor DyLight dye-labeled detection system.

Features:

  • Ready-to-use
  • High sensitivity
  • Low background
  • VectaFluor DyLight 594 labeled anti-goat IgG antibody for detection
  • Excitation: 592 nm
  • Emission: 617 nm
  • Color: Red

Specifications

Unit Size 1 kit
Applications Immunofluorescence
Technology Amplified Fluorescence Systems
Conjugate DyLight 594
Color of Fluorescence Red
Target Species Mouse
Format Ready-to-Use

The post VectaFluor™ Excel Amplified Anti-Mouse IgG, DyLight® 594 Antibody Kit appeared first on VectorLabs.

]]>

Description

VectaFluor Excel Amplified Fluorescent Staining System offers a non-biotin amplification method for fluorescence applications. This system uses a ready-to-use (R.T.U.) Amplifier Antibody, followed by a ready-to-use (R.T.U.) VectaFluor DyLight dye-labeled detection system.

Features:

  • Ready-to-use
  • High sensitivity
  • Low background
  • VectaFluor DyLight 594 labeled anti-goat IgG antibody for detection
  • Excitation: 592 nm
  • Emission: 617 nm
  • Color: Red

Specifications

Unit Size1 kit
ApplicationsImmunofluorescence
TechnologyAmplified Fluorescence Systems
ConjugateDyLight 594
Color of FluorescenceRed
Target SpeciesMouse
FormatReady-to-Use

Kit contents:

  • 15 ml 2.5% Normal Horse Serum for blocking
  • 15 ml Amplifier Antibody (Goat anti-mouse IgG)
  • 15 ml VectaFluor DyLight labeled Horse Anti-Goat IgG

Documents

Citations

Technical Information

All the reagents in the VectaFluor Excel Kit are affinity purified and extensively cross-adsorbed to ensure high sensitivity and low background. In addition, the VectaFluor Reagents are conjugated to the DyLight dyes using technology that ensures maximum labeling without compromising antibody affinity or specificity. DyLight dyes offer several advantages such as brighter fluorescence, greater photostability, and pH independence.

All reagents are supplied in stabilized, R.T.U. solutions for maximum convenience.

Kit Contents:

  • 15 ml 2.5% Normal Horse Serum (ready-to-use) for blocking
  • 15 ml Amplifier Antibody (Goat anti-mouse IgG; ready-to-use)
  • 15 ml VectaFluor DyLight labeled Horse Anti-Goat IgG (ready-to-use)

VectaFluorExcelDiagram jpg

The post VectaFluor™ Excel Amplified Anti-Mouse IgG, DyLight® 594 Antibody Kit appeared first on VectorLabs.

]]> VectaFluor™ Excel Amplified Anti-Rabbit IgG, DyLight® 594 Antibody Kit https://staging.vectorlabs.com/products/vectafluor-excel-dylight-594-anti-rabbit-igg-kit/ Tue, 25 Apr 2023 15:07:25 +0000 https://staging.vectorlabs.com/?post_type=product&p=11729 Description

VectaFluor Excel Amplified Fluorescent Staining System offers a non-biotin amplification method for fluorescence applications. This system uses a ready-to-use (R.T.U.) Amplifier Antibody, followed by a ready-to-use (R.T.U.) VectaFluor DyLight dye-labeled detection system.

Features:

  • High sensitivity
  • Low background
  • VectaFluor DyLight 594 labeled anti-goat IgG antibody for detection
  • Excitation: 592 nm
  • Emission: 617 nm
  • Color: Red

Specifications

Unit Size 1 kit
Applications Immunofluorescence
Technology Amplified Fluorescence Systems
Conjugate DyLight 594
Color of Fluorescence Red
Target Species Rabbit
Format Ready-to-Use

The post VectaFluor™ Excel Amplified Anti-Rabbit IgG, DyLight® 594 Antibody Kit appeared first on VectorLabs.

]]>

Description

VectaFluor Excel Amplified Fluorescent Staining System offers a non-biotin amplification method for fluorescence applications. This system uses a ready-to-use (R.T.U.) Amplifier Antibody, followed by a ready-to-use (R.T.U.) VectaFluor DyLight dye-labeled detection system.

Features:

  • High sensitivity
  • Low background
  • VectaFluor DyLight 594 labeled anti-goat IgG antibody for detection
  • Excitation: 592 nm
  • Emission: 617 nm
  • Color: Red

Specifications

Unit Size1 kit
ApplicationsImmunofluorescence
TechnologyAmplified Fluorescence Systems
ConjugateDyLight 594
Color of FluorescenceRed
Target SpeciesRabbit
FormatReady-to-Use

Kit contents:

  • 15 ml 2.5% Normal Horse Serum for blocking
  • 15 ml Amplifier Antibody (Goat anti-rabbit IgG)
  • 15 ml VectaFluor DyLight labeled Horse Anti-Goat IgG

Documents

Citations

Technical Information

All the reagents in the VectaFluor Excel Kit are affinity purified and extensively cross-adsorbed to ensure high sensitivity and low background. In addition, the VectaFluor Reagents are conjugated to the DyLight dyes using technology that ensures maximum labeling without compromising antibody affinity or specificity. DyLight dyes offer several advantages such as brighter fluorescence, greater photostability, and pH independence.

All reagents are supplied in stabilized, R.T.U. solutions for maximum convenience.

Kit Contents:

  • 15 ml 2.5% Normal Horse Serum (ready-to-use) for blocking
  • 15 ml Amplifier Antibody (Goat anti-rabbit IgG; ready-to-use)
  • 15 ml VectaFluor DyLightt labeled Horse Anti-Goat IgG (ready-to-use)

VectaFluorExcelDiagram jpg

The post VectaFluor™ Excel Amplified Anti-Rabbit IgG, DyLight® 594 Antibody Kit appeared first on VectorLabs.

]]> Goat Anti-Digoxigenin/Digoxin (DIG) Antibody, DyLight™ 594 https://staging.vectorlabs.com/products/dylight-594-anti-digoxigenin-digoxin-dig/ Tue, 25 Apr 2023 14:52:17 +0000 https://staging.vectorlabs.com/?post_type=product&p=11713 Description

Digoxigenin (DIG) is a small plant-derived molecule not found in animals. DIG is used to label nucleic acid probes for applications such as in situ hybridization. DIG-labeled probes are detected with antibodies specifically directed against the DIG label. The DyLight™ conjugated antibodies allow a one-step fluorescent visualization of DIG-labeled probes.

Features:

  • High affinity and highly purified antibodies for FISH
  • Excitation:  592 nm
  • Emission:  617 nm
  • Color:  Red

Specifications

Unit Size 0.5 mg
Applications In situ hybridization
Concentration 1.0 mg active conjugate/ml
Recommended Storage 2-8 °C
Solution 10 mM HEPES, 0.15 M NaCl, pH 7.5, 0.08% sodium azide.
Maximum Emission 617 nm
Maximum Excitation 592 nm
Recommended Usage The recommended concentration range for use is 5-20 µg/ml.
Detection Target Digoxigenin/Digoxin (DIG)
Conjugate DyLight 594
Color of Fluorescence Red
Host Species Goat
Format Concentrate

The post Goat Anti-Digoxigenin/Digoxin (DIG) Antibody, DyLight™ 594 appeared first on VectorLabs.

]]>

Description

Digoxigenin (DIG) is a small plant-derived molecule not found in animals. DIG is used to label nucleic acid probes for applications such as in situ hybridization. DIG-labeled probes are detected with antibodies specifically directed against the DIG label. The DyLight™ conjugated antibodies allow a one-step fluorescent visualization of DIG-labeled probes.

Features:

  • High affinity and highly purified antibodies for FISH
  • Excitation:  592 nm
  • Emission:  617 nm
  • Color:  Red

Specifications

Unit Size0.5 mg
ApplicationsIn situ hybridization
Concentration1.0 mg active conjugate/ml
Recommended Storage2-8 °C
Solution10 mM HEPES, 0.15 M NaCl, pH 7.5, 0.08% sodium azide.
Maximum Emission617 nm
Maximum Excitation592 nm
Recommended UsageThe recommended concentration range for use is 5-20 µg/ml.
Detection TargetDigoxigenin/Digoxin (DIG)
ConjugateDyLight 594
Color of FluorescenceRed
Host SpeciesGoat
FormatConcentrate

Documents

Citations

Technical Information

 

DyLight™ dyes offer several advantages including greater photostability, pH independence and brighter fluorescence. DyLight™ conjugates are completely stable from pH 4 to pH 9, making them compatible with many buffers and diluents.

The post Goat Anti-Digoxigenin/Digoxin (DIG) Antibody, DyLight™ 594 appeared first on VectorLabs.

]]> Horse Anti-Mouse IgG Antibody (H+L), DyLight™ 594 https://staging.vectorlabs.com/products/dylight-594-horse-anti-mouse-igg/ Tue, 25 Apr 2023 14:41:39 +0000 https://staging.vectorlabs.com/?post_type=product&p=11693 Description

Features:

  • Affinity-purified, ultrapure, high affinity antibody
  • Thoroughly adsorbed against serum and immunoglobulins from potentially interfering species
  • Unless otherwise specified, antibodies recognizes both heavy and light chains (H+L)
  • Optimally labeled with DyLight™ 594 to provide the brightest label for fluorescence microscopy
  • Supplied in solution
  • Excitation: 592 nm
  • Emission: 617 nm
  • Color: Red

Specifications

Unit Size 1.5 mg
Applications Immunofluorescence, In situ hybridization, Blotting Applications, Flow Cytometry/Cell Separation
Concentration 1.5 mg active conjugate/ml
Recommended Storage 2-8 °C
Solution 10 mM HEPES, 0.15 M NaCl, pH 7.5, 0.08% sodium azide.
Maximum Emission 617 nm
Maximum Excitation 592 nm
Recommended Usage Recommended concentration range for use 5-20 µg/ml. If this antibody is to be used in tissues which may contain cross-reacting endogenous immunoglobulins, dilution of this antibody may be made in buffers containing 2% normal serum from the same species as the tissue.
Target Species Mouse
Conjugate DyLight 594
Color of Fluorescence Red
Host Species Horse
Format Concentrate

The post Horse Anti-Mouse IgG Antibody (H+L), DyLight™ 594 appeared first on VectorLabs.

]]>

Description

Features:

  • Affinity-purified, ultrapure, high affinity antibody 
  • Thoroughly adsorbed against serum and immunoglobulins from potentially interfering species 
  • Unless otherwise specified, antibodies recognizes both heavy and light chains (H+L) 
  • Optimally labeled with DyLight™ 594 to provide the brightest label for fluorescence microscopy 
  • Supplied in solution 
  • Excitation: 592 nm 
  • Emission: 617 nm 
  • Color: Red

Specifications

Unit Size1.5 mg
ApplicationsImmunofluorescence, In situ hybridization, Blotting Applications, Flow Cytometry/Cell Separation
Concentration1.5 mg active conjugate/ml
Recommended Storage2-8 °C
Solution10 mM HEPES, 0.15 M NaCl, pH 7.5, 0.08% sodium azide.
Maximum Emission617 nm
Maximum Excitation592 nm
Recommended UsageRecommended concentration range for use 5-20 µg/ml. If this antibody is to be used in tissues which may contain cross-reacting endogenous immunoglobulins, dilution of this antibody may be made in buffers containing 2% normal serum from the same species as the tissue.
Target SpeciesMouse
ConjugateDyLight 594
Color of FluorescenceRed
Host SpeciesHorse
FormatConcentrate

Documents

Citations

Technical Information

The anti-mouse Ig antibodies are prepared by hyperimmunizing animals in a manner that produces high affinity antibodies. These are then purified by an affinity chromatography procedure designed to remove any low affinity antibodies which may be present. Cross-reactivities that are likely to interfere with specific labeling are removed by solid-phase adsorption techniques. The final product is then subjected to rigorous quality control assays including immunodiffusion, solid-phase enzyme immunoassays, gel electrophoresis and solid-phase binding assays. In preparing the labeled antibodies, great care is taken to ensure the maximum degree of labeling with no alteration in the specificity and affinity of the antibody. The labeled antibody then undergoes a further series of quality control assays, including immunohistochemical analysis. Unless otherwise specified, our antibodies will recognize both heavy and light chains (H+L).

DyLight™ fluorescent dyes are direct alternatives to traditional fluorophores such as fluorescein (FITC) and rhodamine. The excitation and emission spectra parallel that of other commercially available fluorescent reagents allowing for easy substitution into an existing protocol without requiring any further instrumentation or filter sets.  

DyLight™ dyes offer a number of potential advantages including greater photostability and brighter fluorescence.  DyLight™ dyes are completely stable over a pH range of 4-9 making them compatible with many aqueous-based buffers and diluents. The DyLight™ dyes can be applied as single labels or in combination with other DyLight™ dyes and fluorophores as part of a multiple immunofluorescent antigen staining methodology.  The DyLight™ dyes currently offered are DyLight™ 488 (green), DyLight™ 549 (orange), DyLight™ 594 (red), and DyLight™ 649 (far red).

DyLightspectra jpg
Dylight Table1 jpg

The post Horse Anti-Mouse IgG Antibody (H+L), DyLight™ 594 appeared first on VectorLabs.

]]> Horse Anti-Goat IgG Antibody (H+L), DyLight™ 594 https://staging.vectorlabs.com/products/horse-anti-goat-igg-antibody-hl-dylight-594/ Tue, 25 Apr 2023 04:01:32 +0000 https://staging.vectorlabs.com/?post_type=product&p=11679 Description

DyLight 594 Horse Anti-Goat IgG Antibody can be used for immunofluorescence and other applications. Optimal F/P ratios have been established for each conjugate to ensure maximum fluorescence with minimal background staining.

Features:

  • Affinity-purified, ultrapure, high affinity antibody
  • Suitable for use with goat, sheep, and bovine IgG primary antibodies
  • Thoroughly adsorbed against serum and immunoglobulins from potentially interfering species
  • Recognizes both heavy and light chains (H+L)
  • Optimally labeled with DyLight™ 594 to provide the brightest label for fluorescence microscopy
  • Supplied in solution
  • Excitation: 592 nm
  • Emission: 617 nm
  • Color: Red

Specifications

Unit Size 1.5 mg
Applications Immunofluorescence, In situ hybridization, Blotting Applications, Flow Cytometry/Cell Separation
Concentration 1.5 mg active conjugate/ml
Recommended Storage 2-8 °C
Solution 10 mM HEPES, 0.15 M NaCl, pH 7.5, 0.08% sodium azide.
Maximum Emission 617 nm
Maximum Excitation 592 nm
Recommended Usage Recommended concentration range for use 5-20 µg/ml. If this antibody is to be used in tissues which may contain cross-reacting endogenous immunoglobulins, dilution of this antibody may be made in buffers containing 2% normal serum from the same species as the tissue.
Target Species Goat
Conjugate DyLight 594
Color of Fluorescence Red
Host Species Horse
Format Concentrate

The post Horse Anti-Goat IgG Antibody (H+L), DyLight™ 594 appeared first on VectorLabs.

]]>

Description

DyLight 594 Horse Anti-Goat IgG Antibody can be used for immunofluorescence and other applications. Optimal F/P ratios have been established for each conjugate to ensure maximum fluorescence with minimal background staining.

Features:

  • Affinity-purified, ultrapure, high affinity antibody 
  • Suitable for use with goat, sheep, and bovine IgG primary antibodies
  • Thoroughly adsorbed against serum and immunoglobulins from potentially interfering species 
  • Recognizes both heavy and light chains (H+L) 
  • Optimally labeled with DyLight™ 594 to provide the brightest label for fluorescence microscopy 
  • Supplied in solution 
  • Excitation: 592 nm 
  • Emission: 617 nm 
  • Color: Red

Specifications

Unit Size1.5 mg
ApplicationsImmunofluorescence, In situ hybridization, Blotting Applications, Flow Cytometry/Cell Separation
Concentration1.5 mg active conjugate/ml
Recommended Storage2-8 °C
Solution10 mM HEPES, 0.15 M NaCl, pH 7.5, 0.08% sodium azide.
Maximum Emission617 nm
Maximum Excitation592 nm
Recommended UsageRecommended concentration range for use 5-20 µg/ml. If this antibody is to be used in tissues which may contain cross-reacting endogenous immunoglobulins, dilution of this antibody may be made in buffers containing 2% normal serum from the same species as the tissue.
Target SpeciesGoat
ConjugateDyLight 594
Color of FluorescenceRed
Host SpeciesHorse
FormatConcentrate

Documents

Technical Information

The horse anti-goat Ig antibodies are prepared by hyperimmunizing animals in a manner that produces high affinity antibodies. These are then purified by an affinity chromatography procedure designed to remove any low affinity antibodies which may be present. Cross-reactivities that are likely to interfere with specific labeling are removed by solid-phase adsorption techniques. The final product is then subjected to rigorous quality control assays including immunodiffusion, solid-phase enzyme immunoassays, gel electrophoresis and solid-phase binding assays. In preparing the labeled antibodies, great care is taken to ensure the maximum degree of labeling with no alteration in the specificity and affinity of the antibody. The labeled antibody then undergoes a further series of quality control assays, including immunohistochemical analysis. 

This antibody is suitable for use with goat, sheep, and bovine IgG primary antibodies.

DyLight™ fluorescent dyes are direct alternatives to traditional fluorophores such as fluorescein (FITC) and rhodamine. The excitation and emission spectra parallel that of other commercially available fluorescent reagents allowing for easy substitution into an existing protocol without requiring any further instrumentation or filter sets.  

DyLight™ dyes offer a number of potential advantages including greater photostability and brighter fluorescence.  DyLight™ dyes are completely stable over a pH range of 4-9 making them compatible with many aqueous-based buffers and diluents. The DyLight™ dyes can be applied as single labels or in combination with other DyLight™ dyes and fluorophores as part of a multiple immunofluorescent antigen staining methodology.  The DyLight™ dyes currently offered are DyLight™ 488 (green), DyLight™ 549 (orange), DyLight™ 594 (red), and DyLight™ 649 (far red).

DyLightspectra jpg
Dylight Table1 jpg

The post Horse Anti-Goat IgG Antibody (H+L), DyLight™ 594 appeared first on VectorLabs.

]]> VectaFluor™ Horse Anti-Mouse IgG, DyLight™ 594 Antibody Kit, R.T.U. https://staging.vectorlabs.com/products/vectafluor-dylightr-594-anti-mouse-igg/ Tue, 25 Apr 2023 03:48:47 +0000 https://staging.vectorlabs.com/?post_type=product&p=11648 Description

VectaFluor Ready-to-Use (R.T.U.) DyLight dye-labeled Secondary Antibodies offer maximum convenience for fluorescence staining of cells and tissues. Our affinity purified, extensively cross-adsorbed secondary antibodies are conjugated to DyLight dyes in a manner that ensures maximum degree of labeling without compromising antibody affinity or specificity. DyLight dyes offer several advantages such as brighter fluorescence, greater photostability, and pH independence.

Features:

  • Recognizes both heavy and light chains (H+L)
  • Optimally labeled with DyLight 594 to provide the brightest label for fluorescence microscopy
  • Excitation: 592 nm
  • Emission: 617 nm
  • Color: Red

 

Kit Contents:

  • 15 ml pre-diluted, stabilized secondary antibody solution
  • 15 ml 2.5% normal horse serum for blocking

Specifications

Unit Size 1 kit
Applications Immunofluorescence, In situ hybridization, Flow Cytometry/Cell Separation
Maximum Emission 617 nm
Maximum Excitation 592 nm
Target Species Mouse
Conjugate DyLight 594
Color of Fluorescence Red
Host Species Horse
Format Ready-to-Use

Technical Information

VectaFluor R.T.U. Antibody Reagents are supplied as 15 ml pre-diluted, stabilized, ready-to-use solutions along with 15 ml ready-to-use 2.5% normal horse serum for blocking.

DyLight fluorescent dyes are direct alternatives to traditional fluorophores such as fluorescein (FITC) and rhodamine. The excitation and emission spectra parallel that of other commercially available fluorescent reagents allowing for easy substitution into an existing protocol without requiring any further instrumentation or filter sets.

DyLight dyes offer a number of potential advantages including greater photostability and brighter fluorescence.  DyLight dyes are completely stable over a pH range of 4-9 making them compatible with many aqueous-based buffers and diluents. The DyLight dyes can be applied as single labels or in combination with other DyLight dyes and fluorophores as part of a multiple immunofluorescent antigen staining methodology.  The Dylight dyes currently offered are DyLight 488 (green), DyLight 549 (orange), DyLight 594 (red), and DyLight 649 (far red)

Dylightspectra Jpg Dylightspectra Vectorlabs
Dylight Table1 Jpg Dylight-table1 Vectorlabs

The post VectaFluor™ Horse Anti-Mouse IgG, DyLight™ 594 Antibody Kit, R.T.U. appeared first on VectorLabs.

]]>

Description

VectaFluor Ready-to-Use (R.T.U.) DyLight dye-labeled Secondary Antibodies offer maximum convenience for fluorescence staining of cells and tissues. Our affinity purified, extensively cross-adsorbed secondary antibodies are conjugated to DyLight dyes in a manner that ensures maximum degree of labeling without compromising antibody affinity or specificity. DyLight dyes offer several advantages such as brighter fluorescence, greater photostability, and pH independence.

Features:

  • Recognizes both heavy and light chains (H+L) 
  • Optimally labeled with DyLight 594 to provide the brightest label for fluorescence microscopy 
  • Excitation: 592 nm 
  • Emission: 617 nm 
  • Color: Red

Kit Contents:

  • 15 ml pre-diluted, stabilized secondary antibody solution 
  • 15 ml 2.5% normal horse serum for blocking

Specifications

Unit Size1 kit
ApplicationsImmunofluorescence, In situ hybridization, Flow Cytometry/Cell Separation
Maximum Emission617 nm
Maximum Excitation592 nm
Target SpeciesMouse
ConjugateDyLight 594
Color of FluorescenceRed
Host SpeciesHorse
FormatReady-to-Use

Documents

Technical Information

VectaFluor R.T.U. Antibody Reagents are supplied as 15 ml pre-diluted, stabilized, ready-to-use solutions along with 15 ml ready-to-use 2.5% normal horse serum for blocking.

DyLight fluorescent dyes are direct alternatives to traditional fluorophores such as fluorescein (FITC) and rhodamine. The excitation and emission spectra parallel that of other commercially available fluorescent reagents allowing for easy substitution into an existing protocol without requiring any further instrumentation or filter sets.  

DyLight dyes offer a number of potential advantages including greater photostability and brighter fluorescence.  DyLight dyes are completely stable over a pH range of 4-9 making them compatible with many aqueous-based buffers and diluents. The DyLight dyes can be applied as single labels or in combination with other DyLight dyes and fluorophores as part of a multiple immunofluorescent antigen staining methodology.  The Dylight dyes currently offered are DyLight 488 (green), DyLight 549 (orange), DyLight 594 (red), and DyLight 649 (far red)

DyLightspectra jpg
Dylight Table1 jpg

The post VectaFluor™ Horse Anti-Mouse IgG, DyLight™ 594 Antibody Kit, R.T.U. appeared first on VectorLabs.

]]>