DyLight 549 – VectorLabs https://staging.vectorlabs.com From linker design and synthesis, bioconjugated design and manufacturing, biomolecule labeling and functionalization, and detection system and solutions, our team is here to help you move forward, with impact. Wed, 22 Oct 2025 17:14:19 +0000 en-US hourly 1 https://wordpress.org/?v=6.9 https://staging.vectorlabs.com/wp-content/uploads/2024/11/V-favicon-02-100x100.png DyLight 549 – VectorLabs https://staging.vectorlabs.com 32 32 Streptavidin, DyLight™ 549 https://staging.vectorlabs.com/products/dylight-549-streptavidin/ Sun, 30 Apr 2023 03:48:22 +0000 https://staging.vectorlabs.com/?post_type=product&p=13465 Description

Vector Laboratories fluorochrome-conjugated streptavidin and avidin reagents are highly purified and possess very low non-specific binding properties. They have extremely high affinity for biotin. These fluorescent conjugates can be used to detect biotinylated secondary antibodies and other macromolecules in applications such as immunofluorescence, in situ hybridization, or flow cytometry.

Features:

  • Highly purified and possess very low non-specific binding properties
  • Excitation at 556 nm
  • Emission at 571 nm
  • Color:  Orange

Specifications

Unit Size 1 mg
Applications Immunofluorescence, In situ hybridization, Flow Cytometry/Cell Separation
Recommended Usage 5-30 µg/ml. Avoid using RPMI 1640 or other biotin-containing solutions as diluents. Serum also can contain biotin and should not be added to diluents.
Solution 10 mM HEPES, 0.15 M NaCl, pH 7.5, 0.08% sodium azide.
Maximum Excitation 556 nm
Maximum Emission 571 nm
Recommended Storage 2-8 °C
Concentration 1.0 mg/ml active conjugate
Conjugate DyLight 549
Color of Fluorescence Orange
Format Concentrate

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Description

Vector Laboratories fluorochrome-conjugated streptavidin and avidin reagents are highly purified and possess very low non-specific binding properties. They have extremely high affinity for biotin. These fluorescent conjugates can be used to detect biotinylated secondary antibodies and other macromolecules in applications such as immunofluorescence, in situ hybridization, or flow cytometry.

Features:

  • Highly purified and possess very low non-specific binding properties
  • Excitation at 556 nm
  • Emission at 571 nm
  • Color:  Orange

Specifications

Unit Size1 mg
ApplicationsImmunofluorescence, In situ hybridization, Flow Cytometry/Cell Separation
Recommended Usage5-30 µg/ml. Avoid using RPMI 1640 or other biotin-containing solutions as diluents. Serum also can contain biotin and should not be added to diluents.
Solution10 mM HEPES, 0.15 M NaCl, pH 7.5, 0.08% sodium azide.
Maximum Excitation556 nm
Maximum Emission571 nm
Recommended Storage2-8 °C
Concentration1.0 mg/ml active conjugate
ConjugateDyLight 549
Color of FluorescenceOrange
FormatConcentrate

Documents

Citations

Technical Information

Amplification of fluorescent signals can be easily achieved with our biotinylated secondary antibodies followed by our highly purified fluorochrome-labeled streptavidin or avidin. Using a biotin/avidin or biotin/streptavidin detection system results in an additional layer of amplification over a directly conjugated secondary antibody.

DyLight™ dyes offer several advantages including greater photostability, pH independence, and brighter fluorescence. We offer DyLight™ conjugated streptavidin for use in a variety of applications, in particular, cell- and tissue-based immunofluorescent detection. The DyLight™ conjugates are completely stable from pH 4 to pH 9, making them compatible with many aqueous-based buffers and diluents.

DyLightspectra jpg

Dylight Table1

The post Streptavidin, DyLight™ 549 appeared first on VectorLabs.

]]> Glysite™ Scout Glycan Screening Kit, Immunofluorescence 594 https://staging.vectorlabs.com/products/glysite-scout-screening-kit-594/ Sun, 30 Apr 2023 03:28:46 +0000 https://staging.vectorlabs.com/?post_type=product&p=13436 Description

Glysite Scout Glycan Screening Kit, Immunofluorescence 594 is a fully integrated kit for the detection of glycan expression in tissue sections. Included in the kit is a diverse panel of glycan binders (lectins) ideal for the preliminary screening of tissue samples with unknown glycosylation changes with specificity to mannose, complex N-glycan, core O-glycan, fucose, sialic acid, sulfation, GlcNAc, chitin, galactose, and LacNAc.

Validated on human and mouse tissue—including FFPE tissue, across a range of tissue types including colon, lung, spleen, kidney, liver, pancreas, testes, heart, bladder, and uterus—this versatile kit is flexible to meet your needs. Glysite Scout Glycan Screening Kits, Immunofluorescence are available with 3 fluorophore options, 488 (GSK-3000), 594 (GSK-2000), and 649 (GSK-1000).

Specifications

Unit Size 1 kit
Applications Immunofluorescence, Glycobiology
Recommended Storage Store reagents in original bottles at 2–8 °C. Do not freeze the kit.
Conjugate DyLight 549
Sugar Specificity Mannose, Galactose, Fucose, Sialic Acid

 

Kit Components

Kit Contents:

This glycan screening kit is designed to provide the investigator with a panel of eight biotinylated lectin conjugates. The lectins have been selected to offer a variety of sugar specificities and are of the same high quality as the reagents offered individually.

Glysite Scout Glycan Screening Kit, Immunofluorescence 594 contains the following biotinylated lectins:

  • AAL (Aleuria Aurantia)
  • ECL, ECA (Erythrina Cristagalli)
  • GNL (Galanthus Nivalis)
  • Jacalin
  • MAL II (Maackia Amurensis II)
  • PHA-L (Phaseolus Vulgaris Leucoagglutinin)
  • WFA, WFL (Wisteria Fluoribunda)
  • WGA (Wheat Germ Agglutinin)

In addition to the glycan binders, the kit includes optimized immunofluorescence reagents for glycan detection, including blocking solutions, DyLight™ streptavidin, and VECTASHIELD Vibrance® Antifade Mounting Medium with DAPI.

The post Glysite™ Scout Glycan Screening Kit, Immunofluorescence 594 appeared first on VectorLabs.

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Resources

Description

Glysite Scout Glycan Screening Kit, Immunofluorescence 594 is a fully integrated kit for the detection of glycan expression in tissue sections. Included in the kit is a diverse panel of glycan binders (lectins) ideal for the preliminary screening of tissue samples with unknown glycosylation changes with specificity to mannose, complex N-glycan, core O-glycan, fucose, sialic acid, sulfation, GlcNAc, chitin, galactose, and LacNAc.

Validated on human and mouse tissue—including FFPE tissue, across a range of tissue types including colon, lung, spleen, kidney, liver, pancreas, testes, heart, bladder, and uterus—this versatile kit is flexible to meet your needs. Glysite Scout Glycan Screening Kits, Immunofluorescence are available with 3 fluorophore options, 488 (GSK-3000), 594 (GSK-2000), and 649 (GSK-1000).

Specifications

Unit Size1 kit
ApplicationsImmunofluorescence, Glycobiology
Recommended StorageStore reagents in original bottles at 2–8 °C. Do not freeze the kit.
ConjugateDyLight 549
Sugar SpecificityMannose, Galactose, Fucose, Sialic Acid

Kit Components

Kit Contents:

This glycan screening kit is designed to provide the investigator with a panel of eight biotinylated lectin conjugates. The lectins have been selected to offer a variety of sugar specificities and are of the same high quality as the reagents offered individually.

Glysite Scout Glycan Screening Kit, Immunofluorescence 594 contains the following biotinylated lectins:

  • AAL (Aleuria Aurantia)
  • ECL, ECA (Erythrina Cristagalli)
  • GNL (Galanthus Nivalis)
  • Jacalin
  • MAL II (Maackia Amurensis II)
  • PHA-L (Phaseolus Vulgaris Leucoagglutinin)
  • WFA, WFL (Wisteria Fluoribunda)
  • WGA (Wheat Germ Agglutinin)

In addition to the glycan binders, the kit includes optimized immunofluorescence reagents for glycan detection, including blocking solutions, DyLight™ streptavidin, and VECTASHIELD Vibrance® Antifade Mounting Medium with DAPI. 

Documents

Product FAQs

Virtually any tissue type and species are suitable for use with a Glysite Scout Glycan Screening Kit, Immunofluorescence, as glycans are ubiquitous in nature and in life. The kit has been validated on human and mouse tissue—including FFPE tissue, across a range of organs, including colon, lung, spleen, kidney, liver, pancreas, testes, heart, bladder, and uterus.

Each of the three Glysite Scout Glycan Screening Kits, Immunofluorescence contain the same lectins but with a different fluorescent streptavidin detection reagent, offering a choice of Dylight™ 488 (GSK-3000), Dylight™ 594 (GSK-2000), or Dylight™ 649 (GSK-1000). This allows users to choose the kit which best matches the capabilities of their fluorescent microscope and also provides flexibility for multiplexing with other fluorescent detection reagents they might already be using. 

VECTASHIELD Vibrance is an antifade medium for immunofluorescence applications with setting properties, which means the coverslip is immobilized after curing and does not require sealing. Additionally, slides can be stored at room temperature, enabling extended archiving time. VECTASHIELD PLUS does not have setting properties and remains molten, and slides must be stored at 2–8°C. Both VECTASHIELD Vibrance and VECTASHIELD PLUS have superior antifade/anti-photobleaching properties across the spectrum and are compatible with commonly used fluorophores. 

The need for an antigen retrieval step in a staining protocol depends on multiple variables, including the target glycan and its location in the specimen, the glycan-binder, the type of tissue, and the method and length of fixation. Investigators should run controls with and without antigen retrieval to determine if this step is needed for their specimen. 

Tissue components such as red blood cells, elastin, and collagen are strongly fluorescent, particularly in FFPE sections, making it difficult to discern between relevant signal and background. Formalin fixation tends to induce a significant amount of fluorescence to a specimen. The Vector TrueVIEW Autofluorescence Quenching Kit provides a dramatic reduction of autofluorescence, allowing a clear view of the desired signal, so it was utilized as needed on the specimens represented in the images. 

If co-labeling with a primary antibody, users should optimize each protocol separately to achieve maximum signal to noise ratios. Once this is complete, the two protocols can be combined, with the Glysite Scout Glycan Screening Kit protocol applied first, followed by the antibody staining protocol. Users should perform the proper controls to demonstrate that the reagents in each protocol are not cross–reacting with each other. 

The Carbo-Free Blocking Solution included in the Glysite Scout Glycan Screening Kit is virtually free of glycoproteins or other glycosylated molecules. This is critical since these kits detect glycans and would therefore bind to blocking reagents which contained glycosylated components. Users should not use other blocking solutions (e.g., milk derivatives, sera) as it may lead to high background. 

Each Glysite Scout Glycan Screening Kit contains enough reagents for approximately 50 tissue samples per lectin, or up to 400 samples if using one lectin per tissue sample (8 lectins x 50 tissue samples). 

The lectins were selected based upon their broad applicability and specificity to frequently cited glycan targets from literature sources, spanning fields of Cancer, Immunology, Metabolism, Inflammation, Virology, and Tumor Microenvironment, among others. They enable detection of all the major glycan motifs for the evaluation of glycan distribution in a target specimen. 

Technical Information

The lectins selected for the Glysite Scout Glycan Screening Kit, Immunofluorescence 594 were specifically chosen to provide detection of all of the major glycan motifs for the evaluation of glycan distribution in a target specimen. Follow our optimized protocol or view our how-to video to learn how you can rapidly explore glycan expression in your tissue section.

In addition to the glycan binders, the kit includes optimized immunofluorescence reagents for glycan detection, including blocking solutions, DyLight™ streptavidin, and VECTASHIELD Vibrance® Antifade Mounting Medium with DAPI. 

Legal Information

DyLight is a trademark of Pierce Biotechnology, Inc.

The post Glysite™ Scout Glycan Screening Kit, Immunofluorescence 594 appeared first on VectorLabs.

]]> Horse Anti-Mouse IgG Antibody (H+L), DyLight™ 549 https://staging.vectorlabs.com/products/dylight-549-horse-anti-mouse-igg/ Tue, 25 Apr 2023 03:25:12 +0000 https://staging.vectorlabs.com/?post_type=product&p=11610 Description

DyLight 549 Horse Anti-Mouse IgG Antibody can be used for immunofluorescence and other applications. Optimal F/P ratios have been established for each conjugate to ensure maximum fluorescence with minimal background staining.

Features:

  • Recognizes both heavy and light chains (H+L)
  • Optimally labeled with DyLight™ 549 to provide the brightest label for fluorescence microscopy
  • Supplied in solution
  • Excitation: 556 nm
  • Emission: 571 nm
  • Color: Orange

Specifications

Unit Size 1.5 mg
Applications Immunofluorescence, In situ hybridization, Blotting Applications, Flow Cytometry/Cell Separation
Concentration 1.5 mg active conjugate/ml
Recommended Storage 2-8 °C
Solution 10 mM HEPES, 0.15 M NaCl, pH 7.5, 0.08% sodium azide.
Maximum Emission 571 nm
Maximum Excitation 556 nm
Recommended Usage Recommended concentration range for use 5-20 µg/ml. If this antibody is to be used in tissues which may contain cross-reacting endogenous immunoglobulins, dilution of this antibody may be made in buffers containing 2% normal serum from the same species as the tissue.
Target Species Mouse
Conjugate DyLight 549
Color of Fluorescence Orange
Host Species Horse
Format Concentrate

Technical Information

The anti-mouse Ig antibodies are prepared by hyperimmunizing animals in a manner that produces high affinity antibodies. These are then purified by an affinity chromatography procedure designed to remove any low affinity antibodies which may be present. Cross-reactivities that are likely to interfere with specific labeling are removed by solid-phase adsorption techniques. The final product is then subjected to rigorous quality control assays including immunodiffusion, solid-phase enzyme immunoassays, gel electrophoresis and solid-phase binding assays. In preparing the labeled antibodies, great care is taken to ensure the maximum degree of labeling with no alteration in the specificity and affinity of the antibody. The labeled antibody then undergoes a further series of quality control assays, including immunohistochemical analysis.

DyLight™ fluorescent dyes are direct alternatives to traditional fluorophores such as fluorescein (FITC) and rhodamine. The excitation and emission spectra parallel that of other commercially available fluorescent reagents allowing for easy substitution into an existing protocol without requiring any further instrumentation or filter sets.

DyLight™ dyes offer a number of potential advantages including greater photostability and brighter fluorescence.  DyLight™ dyes are completely stable over a pH range of 4-9 making them compatible with many aqueous-based buffers and diluents. The DyLight™ dyes can be applied as single labels or in combination with other DyLight™ dyes and fluorophores as part of a multiple immunofluorescent antigen staining methodology.  The DyLight™ dyes currently offered are DyLight™ 488 (green), DyLight™ 549 (orange), DyLight™ 594 (red), and DyLight™ 649 (far red).

Dylightspectra Jpg Dylightspectra Vectorlabs
Dylight Table1 Jpg Dylight-table1 Vectorlabs

The post Horse Anti-Mouse IgG Antibody (H+L), DyLight™ 549 appeared first on VectorLabs.

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Description

DyLight 549 Horse Anti-Mouse IgG Antibody can be used for immunofluorescence and other applications. Optimal F/P ratios have been established for each conjugate to ensure maximum fluorescence with minimal background staining.

Features:

  • Recognizes both heavy and light chains (H+L) 
  • Optimally labeled with DyLight™ 549 to provide the brightest label for fluorescence microscopy 
  • Supplied in solution 
  • Excitation: 556 nm 
  • Emission: 571 nm 
  • Color: Orange

Specifications

Unit Size1.5 mg
ApplicationsImmunofluorescence, In situ hybridization, Blotting Applications, Flow Cytometry/Cell Separation
Concentration1.5 mg active conjugate/ml
Recommended Storage2-8 °C
Solution10 mM HEPES, 0.15 M NaCl, pH 7.5, 0.08% sodium azide.
Maximum Emission571 nm
Maximum Excitation556 nm
Recommended UsageRecommended concentration range for use 5-20 µg/ml. If this antibody is to be used in tissues which may contain cross-reacting endogenous immunoglobulins, dilution of this antibody may be made in buffers containing 2% normal serum from the same species as the tissue.
Target SpeciesMouse
ConjugateDyLight 549
Color of FluorescenceOrange
Host SpeciesHorse
FormatConcentrate

Documents

Citations

Technical Information

The anti-mouse Ig antibodies are prepared by hyperimmunizing animals in a manner that produces high affinity antibodies. These are then purified by an affinity chromatography procedure designed to remove any low affinity antibodies which may be present. Cross-reactivities that are likely to interfere with specific labeling are removed by solid-phase adsorption techniques. The final product is then subjected to rigorous quality control assays including immunodiffusion, solid-phase enzyme immunoassays, gel electrophoresis and solid-phase binding assays. In preparing the labeled antibodies, great care is taken to ensure the maximum degree of labeling with no alteration in the specificity and affinity of the antibody. The labeled antibody then undergoes a further series of quality control assays, including immunohistochemical analysis. 

DyLight™ fluorescent dyes are direct alternatives to traditional fluorophores such as fluorescein (FITC) and rhodamine. The excitation and emission spectra parallel that of other commercially available fluorescent reagents allowing for easy substitution into an existing protocol without requiring any further instrumentation or filter sets.  

DyLight™ dyes offer a number of potential advantages including greater photostability and brighter fluorescence.  DyLight™ dyes are completely stable over a pH range of 4-9 making them compatible with many aqueous-based buffers and diluents. The DyLight™ dyes can be applied as single labels or in combination with other DyLight™ dyes and fluorophores as part of a multiple immunofluorescent antigen staining methodology.  The DyLight™ dyes currently offered are DyLight™ 488 (green), DyLight™ 549 (orange), DyLight™ 594 (red), and DyLight™ 649 (far red).

DyLightspectra jpg
Dylight Table1 jpg

The post Horse Anti-Mouse IgG Antibody (H+L), DyLight™ 549 appeared first on VectorLabs.

]]> Goat Anti-Rabbit IgG Antibody (H+L), DyLight™ 549 https://staging.vectorlabs.com/products/dylight-549-goat-anti-rabbit-igg/ Mon, 24 Apr 2023 19:47:39 +0000 https://staging.vectorlabs.com/?post_type=product&p=11521 Description

DyLight 549 Goat Anti-Rabbit IgG Antibody can be used for immunofluorescence and other applications. Optimal F/P ratios have been established for each conjugate to ensure maximum fluorescence with minimal background staining.

Features:

  • Recognizes both heavy and light chains (H+L)
  • Optimally labeled with DyLight™ 549 to provide the brightest label for fluorescence microscopy
  • Supplied in solution
  • Excitation: 556 nm
  • Emission: 571 nm
  • Color: Orange

Specifications

Unit Size 1.5 mg
Applications Immunofluorescence, In situ hybridization, Blotting Applications, Flow Cytometry/Cell Separation
Concentration 1.5 mg active conjugate/ml
Recommended Storage 2-8 °C
Solution 10 mM HEPES, 0.15 M NaCl, pH 7.5, 0.08% sodium azide.
Maximum Emission 571 nm
Maximum Excitation 556 nm
Recommended Usage Recommended concentration range for use 5-20 µg/ml. If this antibody is to be used in tissues which may contain cross-reacting endogenous immunoglobulins, dilution of this antibody may be made in buffers containing 2% normal serum from the same species as the tissue.
Target Species Rabbit
Conjugate DyLight 549
Color of Fluorescence Orange
Host Species Goat
Format Concentrate

The post Goat Anti-Rabbit IgG Antibody (H+L), DyLight™ 549 appeared first on VectorLabs.

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Description

DyLight 549 Goat Anti-Rabbit IgG Antibody can be used for immunofluorescence and other applications. Optimal F/P ratios have been established for each conjugate to ensure maximum fluorescence with minimal background staining.

Features:

  • Recognizes both heavy and light chains (H+L)
  • Optimally labeled with DyLight™ 549 to provide the brightest label for fluorescence microscopy
  • Supplied in solution
  • Excitation: 556 nm
  • Emission: 571 nm
  • Color: Orange

Specifications

Unit Size1.5 mg
ApplicationsImmunofluorescence, In situ hybridization, Blotting Applications, Flow Cytometry/Cell Separation
Concentration1.5 mg active conjugate/ml
Recommended Storage2-8 °C
Solution10 mM HEPES, 0.15 M NaCl, pH 7.5, 0.08% sodium azide.
Maximum Emission571 nm
Maximum Excitation556 nm
Recommended UsageRecommended concentration range for use 5-20 µg/ml. If this antibody is to be used in tissues which may contain cross-reacting endogenous immunoglobulins, dilution of this antibody may be made in buffers containing 2% normal serum from the same species as the tissue.
Target SpeciesRabbit
ConjugateDyLight 549
Color of FluorescenceOrange
Host SpeciesGoat
FormatConcentrate

Documents

Citations

Technical Information

The goat anti-rabbit Ig antibodies are prepared by hyperimmunizing animals in a manner that produces high affinity antibodies. These are then purified by an affinity chromatography procedure designed to remove any low affinity antibodies which may be present. Cross-reactivities that are likely to interfere with specific labeling are removed by solid-phase adsorption techniques. The final product is then subjected to rigorous quality control assays including immunodiffusion, solid-phase enzyme immunoassays, gel electrophoresis and solid-phase binding assays. In preparing the labeled antibodies, great care is taken to ensure the maximum degree of labeling with no alteration in the specificity and affinity of the antibody. The labeled antibody then undergoes a further series of quality control assays, including immunohistochemical analysis. 

DyLight™ fluorescent dyes are direct alternatives to traditional fluorophores such as fluorescein (FITC) and rhodamine. The excitation and emission spectra parallel that of other commercially available fluorescent reagents allowing for easy substitution into an existing protocol without requiring any further instrumentation or filter sets.  

DyLight™ dyes offer a number of potential advantages including greater photostability and brighter fluorescence.  DyLight™ dyes are completely stable over a pH range of 4-9 making them compatible with many aqueous-based buffers and diluents. The DyLight™ dyes can be applied as single labels or in combination with other DyLight™ dyes and fluorophores as part of a multiple immunofluorescent antigen staining methodology.  The DyLight™ dyes currently offered are DyLight™ 488 (green), DyLight™ 549 (orange), DyLight™ 594 (red), and DyLight™ 649 (far red).

DyLightspectra jpg
Dylight Table1 jpg

The post Goat Anti-Rabbit IgG Antibody (H+L), DyLight™ 549 appeared first on VectorLabs.

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