DyLight 488 – VectorLabs https://staging.vectorlabs.com From linker design and synthesis, bioconjugated design and manufacturing, biomolecule labeling and functionalization, and detection system and solutions, our team is here to help you move forward, with impact. Wed, 22 Oct 2025 17:14:19 +0000 en-US hourly 1 https://wordpress.org/?v=6.9 https://staging.vectorlabs.com/wp-content/uploads/2024/11/V-favicon-02-100x100.png DyLight 488 – VectorLabs https://staging.vectorlabs.com 32 32 Anti-Streptavidin Antibody, DyLight® 488 https://staging.vectorlabs.com/products/dylight-488-anti-streptavidin/ Tue, 02 May 2023 02:35:55 +0000 https://staging.vectorlabs.com/?post_type=product&p=13944 Description

Anti-Streptavidin antibodies have been widely used as amplifying reagents in immunohistochemistry, in situ hybridization, microarray assays, ELISAs, blots, and many other applications. Our antibodies to streptavidin are produced in goats using our highly purified streptavidin and isolated by affinity chromatography. Anti-Streptavidin does not bind avidin and Anti-Avidin does not recognize streptavidin. These antibodies provide opportunities to significantly amplify signals in many applications.

DyLight 488 Anti-Streptavidin has been optimally labeled to provide maximum fluorescence.

  • Maximum Excitation: 493 nm
  • Maximum Emission: 518 nm
  • Color: green

Specifications

Unit Size 0.5 mg
Applications Immunofluorescence, In situ hybridization
Concentration 1 mg active conjugate/ml
Recommended Storage 2-8 °C
Solution 10 mM HEPES, pH 7.5, 0.15 M NaCl, 0.08% sodium azide
Recommended Usage The recommended concentration range for use is 5-20 µg/ml.
Detection Target Streptavidin
Conjugate DyLight 488
Color of Fluorescence Green
Host Species Goat
Format Concentrate

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Description

Anti-Streptavidin antibodies have been widely used as amplifying reagents in immunohistochemistry, in situ hybridization, microarray assays, ELISAs, blots, and many other applications. Our antibodies to streptavidin are produced in goats using our highly purified streptavidin and isolated by affinity chromatography. Anti-Streptavidin does not bind avidin and Anti-Avidin does not recognize streptavidin. These antibodies provide opportunities to significantly amplify signals in many applications.

DyLight 488 Anti-Streptavidin has been optimally labeled to provide maximum fluorescence.

  • Maximum Excitation: 493 nm
  • Maximum Emission: 518 nm
  • Color: green

Specifications

Unit Size0.5 mg
ApplicationsImmunofluorescence, In situ hybridization
Concentration1 mg active conjugate/ml
Recommended Storage2-8 °C
Solution10 mM HEPES, pH 7.5, 0.15 M NaCl, 0.08% sodium azide
Recommended UsageThe recommended concentration range for use is 5-20 µg/ml.
Detection TargetStreptavidin
ConjugateDyLight 488
Color of FluorescenceGreen
Host SpeciesGoat
FormatConcentrate

Documents

Citations

Technical Information

The capability of binding avidin or streptavidin via either biotin binding sites or through antigen binding sites, makes these biotinylated antibodies unique. These antibodies can be used either as part of preformed complexes or in sequence to amplify fluorescent signals. When used in sequence, the target is first labeled with fluorochrome-conjugated avidin or streptavidin, followed by incubation with Biotinylated Anti-Avidin or Biotinylated Anti-Streptavidin, followed by a second layer of fluorochrome-conjugated avidin or streptavidin. This sequence can be repeated. This multi-layered approach introduces more fluorochromes at the target site and can provide a multi-fold amplification over a single layer.

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]]> Streptavidin, DyLight® 488 https://staging.vectorlabs.com/products/dylight-488-streptavidin/ Mon, 01 May 2023 19:35:55 +0000 https://staging.vectorlabs.com/?post_type=product&p=13562 Description

DyLight 488 Streptavidin can be used to detect biotinylated secondary antibodies and other macromolecules in applications such as immunofluorescence, in situ hybridization, or flow cytometry.

Features:

  • Advantages of DyLight™ dyes include greater photostability, pH independence, and brighter fluorescence
  • Excitation maximum: 493 nm
  • Emission maximum: 518 nm
  • Color:  Green

Specifications

Unit Size 1 mg
Applications Immunofluorescence, In situ hybridization, Flow Cytometry/Cell Separation
Recommended Usage Recommended concentration range for use: 5-30 µg/ml. Avoid using RPMI 1640 or other biotin-containing solutions as diluents. Serum also can contain biotin and should not be added to diluents.
Solution 10 mM HEPES, 0.15 M NaCl, pH 7.5, 0.08% sodium azide.
Maximum Excitation 493 nm
Maximum Emission 518 nm
Recommended Storage 2-8 °C
Concentration 1.0 mg/ml active conjugate
Conjugate DyLight 488
Color of Fluorescence Green
Format Concentrate

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Description

DyLight 488 Streptavidin can be used to detect biotinylated secondary antibodies and other macromolecules in applications such as immunofluorescence, in situ hybridization, or flow cytometry. 

Features:

  • Advantages of DyLight™ dyes include greater photostability, pH independence, and brighter fluorescence
  • Excitation maximum: 493 nm
  • Emission maximum: 518 nm
  • Color:  Green

Specifications

Unit Size1 mg
ApplicationsImmunofluorescence, In situ hybridization, Flow Cytometry/Cell Separation
Recommended UsageRecommended concentration range for use: 5-30 µg/ml. Avoid using RPMI 1640 or other biotin-containing solutions as diluents. Serum also can contain biotin and should not be added to diluents.
Solution10 mM HEPES, 0.15 M NaCl, pH 7.5, 0.08% sodium azide.
Maximum Excitation493 nm
Maximum Emission518 nm
Recommended Storage2-8 °C
Concentration1.0 mg/ml active conjugate
ConjugateDyLight 488
Color of FluorescenceGreen
FormatConcentrate

Documents

Citations

Technical Information

Vector Laboratories fluorochrome-conjugated streptavidin and avidin reagents are highly purified and possess very low non-specific binding properties. They have extremely high affinity for biotin. 

Amplification of fluorescent signals can be easily achieved with our biotinylated secondary antibodies followed by our highly purified fluorochrome-labeled streptavidin or avidin. Using a biotin/avidin or biotin/streptavidin detection system results in an additional layer of amplification over a directly conjugated secondary antibody.

DyLight™ dyes offer several advantages including greater photostability, pH independence, and brighter fluorescence. We offer DyLight™ conjugated streptavidin for use in a variety of applications, in particular, cell- and tissue-based immunofluorescent detection. The DyLight™ conjugates are completely stable from pH 4 to pH 9, making them compatible with many aqueous-based buffers and diluents.

DyLightspectra jpg

Dylight Table1

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]]> Goat Anti-Digoxigenin/Digoxin (DIG) Antibody, DyLight™ 488 https://staging.vectorlabs.com/products/dylight-488-anti-digoxigenin-digoxin-dig/ Mon, 01 May 2023 19:02:49 +0000 https://staging.vectorlabs.com/?post_type=product&p=13538 Description

Digoxigenin (DIG) is a small plant-derived molecule not found in animals. DIG is used to label nucleic acid probes for applications such as in situ hybridization. DIG-labeled probes are detected with antibodies specifically directed against the DIG label. We have generated high affinity and highly purified antibodies for this purpose. The DyLight™ conjugated antibodies allow a one-step fluorescent visualization of DIG-labeled probes.

Features:

  • High affinity and highly purified antibody to detect DIG-labed probes
  • DyLight® 488 conjugated antibodies allow a one-step fluorescent visualization of DIG-labeled probes
  • Excitation:  493 nm
  • Emission:  518 nm
  • Color:  Green

Specifications

Unit Size 0.5 mg
Applications In situ hybridization
Concentration 1.0 mg active conjugate/ml
Recommended Storage 2-8 °C
Solution 10 mM HEPES, 0.15 M NaCl, pH 7.8, 0.08% sodium azide.
Maximum Emission 518 nm
Maximum Excitation 493 nm
Recommended Usage The recommended concentration range for use is 5-20 µg/ml.
Detection Target Digoxigenin/Digoxin (DIG)
Conjugate DyLight 488
Color of Fluorescence Green
Host Species Goat
Format Concentrate

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Description

Digoxigenin (DIG) is a small plant-derived molecule not found in animals. DIG is used to label nucleic acid probes for applications such as in situ hybridization. DIG-labeled probes are detected with antibodies specifically directed against the DIG label. We have generated high affinity and highly purified antibodies for this purpose. The DyLight™ conjugated antibodies allow a one-step fluorescent visualization of DIG-labeled probes.

Features:

  • High affinity and highly purified antibody to detect DIG-labed probes
  • DyLight® 488 conjugated antibodies allow a one-step fluorescent visualization of DIG-labeled probes
  • Excitation:  493 nm
  • Emission:  518 nm
  • Color:  Green

Specifications

Unit Size0.5 mg
ApplicationsIn situ hybridization
Concentration1.0 mg active conjugate/ml
Recommended Storage2-8 °C
Solution10 mM HEPES, 0.15 M NaCl, pH 7.8, 0.08% sodium azide.
Maximum Emission518 nm
Maximum Excitation493 nm
Recommended UsageThe recommended concentration range for use is 5-20 µg/ml.
Detection TargetDigoxigenin/Digoxin (DIG)
ConjugateDyLight 488
Color of FluorescenceGreen
Host SpeciesGoat
FormatConcentrate

Documents

Citations

Technical Information

DyLight™ dyes offer several advantages including greater photostability, pH independence and brighter fluorescence. DyLight™ conjugates are completely stable from pH 4 to pH 9, making them compatible with many buffers and diluents.

The post Goat Anti-Digoxigenin/Digoxin (DIG) Antibody, DyLight™ 488 appeared first on VectorLabs.

]]> Glysite™ Scout Glycan Screening Kit, Immunofluorescence 488 https://staging.vectorlabs.com/products/glysite-scout-screening-kit-488/ Sun, 30 Apr 2023 03:33:40 +0000 https://staging.vectorlabs.com/?post_type=product&p=13446 Description

Glysite Scout Glycan Screening Kit, Immunofluorescence 488 is a fully integrated kit for the detection of glycan expression in tissue sections. Included in the kit is a diverse panel of glycan binders (lectins) ideal for the preliminary screening of tissue samples with unknown glycosylation changes with specificity to mannose, complex N-glycan, core O-glycan, fucose, sialic acid, sulfation, GlcNAc, chitin, galactose, and LacNAc.

Validated on human and mouse tissue—including FFPE tissue, across a range of tissue types including colon, lung, spleen, kidney, liver, pancreas, testes, heart, bladder, and uterus—this versatile kit is flexible to meet your needs. Glysite Scout Glycan Screening Kits, Immunofluorescence are available with 3 fluorophore options, 488 (GSK-3000), 594 (GSK-2000), and 649 (GSK-1000).

Specifications

Unit Size 1 kit
Applications Immunofluorescence, Glycobiology
Recommended Storage Store reagents in original bottles at 2–8 °C. Do not freeze the kit.
Conjugate DyLight 488
Sugar Specificity Mannose, Galactose, Fucose, Sialic Acid

Kit Components

This glycan screening kit is designed to provide the investigator with a panel of eight biotinylated lectin conjugates. The lectins have been selected to offer a variety of sugar specificities and are of the same high quality as the reagents offered individually.

Glysite Scout Glycan Screening Kit, Immunofluorescence 488 contains the following biotinylated lectins:

  • AAL (Aleuria Aurantia)
  • ECL, ECA (Erythrina Cristagalli)
  • GNL (Galanthus Nivalis)
  • Jacalin
  • MAL II (Maackia Amurensis II)
  • PHA-L (Phaseolus Vulgaris Leucoagglutinin)
  • WFA, WFL (Wisteria Fluoribunda)
  • WGA (Wheat Germ Agglutinin)

 

In addition to the glycan binders, the kit includes optimized immunofluorescence reagents for glycan detection, including blocking solutions, DyLight™ streptavidin, and VECTASHIELD Vibrance® Antifade Mounting Medium with DAPI.

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Resources

Description

Glysite Scout Glycan Screening Kit, Immunofluorescence 488 is a fully integrated kit for the detection of glycan expression in tissue sections. Included in the kit is a diverse panel of glycan binders (lectins) ideal for the preliminary screening of tissue samples with unknown glycosylation changes with specificity to mannose, complex N-glycan, core O-glycan, fucose, sialic acid, sulfation, GlcNAc, chitin, galactose, and LacNAc.

Validated on human and mouse tissue—including FFPE tissue, across a range of tissue types including colon, lung, spleen, kidney, liver, pancreas, testes, heart, bladder, and uterus—this versatile kit is flexible to meet your needs. Glysite Scout Glycan Screening Kits, Immunofluorescence are available with 3 fluorophore options, 488 (GSK-3000), 594 (GSK-2000), and 649 (GSK-1000).

Specifications

Unit Size1 kit
ApplicationsImmunofluorescence, Glycobiology
Recommended StorageStore reagents in original bottles at 2–8 °C. Do not freeze the kit.
ConjugateDyLight 488
Sugar SpecificityMannose, Galactose, Fucose, Sialic Acid

Kit Components

This glycan screening kit is designed to provide the investigator with a panel of eight biotinylated lectin conjugates. The lectins have been selected to offer a variety of sugar specificities and are of the same high quality as the reagents offered individually.

Glysite Scout Glycan Screening Kit, Immunofluorescence 488 contains the following biotinylated lectins:

  • AAL (Aleuria Aurantia)
  • ECL, ECA (Erythrina Cristagalli)
  • GNL (Galanthus Nivalis)
  • Jacalin
  • MAL II (Maackia Amurensis II)
  • PHA-L (Phaseolus Vulgaris Leucoagglutinin)
  • WFA, WFL (Wisteria Fluoribunda)
  • WGA (Wheat Germ Agglutinin)

In addition to the glycan binders, the kit includes optimized immunofluorescence reagents for glycan detection, including blocking solutions, DyLight™ streptavidin, and VECTASHIELD Vibrance® Antifade Mounting Medium with DAPI. 

Documents

Product FAQs

Virtually any tissue type and species are suitable for use with a Glysite Scout Glycan Screening Kit, Immunofluorescence, as glycans are ubiquitous in nature and in life. The kit has been validated on human and mouse tissue—including FFPE tissue, across a range of organs, including colon, lung, spleen, kidney, liver, pancreas, testes, heart, bladder, and uterus.

Each of the three Glysite Scout Glycan Screening Kits, Immunofluorescence contain the same lectins but with a different fluorescent streptavidin detection reagent, offering a choice of Dylight™ 488 (GSK-3000), Dylight™ 594 (GSK-2000), or Dylight™ 649 (GSK-1000). This allows users to choose the kit which best matches the capabilities of their fluorescent microscope and also provides flexibility for multiplexing with other fluorescent detection reagents they might already be using. 

VECTASHIELD Vibrance is an antifade medium for immunofluorescence applications with setting properties, which means the coverslip is immobilized after curing and does not require sealing. Additionally, slides can be stored at room temperature, enabling extended archiving time. VECTASHIELD PLUS does not have setting properties and remains molten, and slides must be stored at 2–8°C. Both VECTASHIELD Vibrance and VECTASHIELD PLUS have superior antifade/anti-photobleaching properties across the spectrum and are compatible with commonly used fluorophores. 

The need for an antigen retrieval step in a staining protocol depends on multiple variables, including the target glycan and its location in the specimen, the glycan-binder, the type of tissue, and the method and length of fixation. Investigators should run controls with and without antigen retrieval to determine if this step is needed for their specimen. 

Tissue components such as red blood cells, elastin, and collagen are strongly fluorescent, particularly in FFPE sections, making it difficult to discern between relevant signal and background. Formalin fixation tends to induce a significant amount of fluorescence to a specimen. The Vector TrueVIEW Autofluorescence Quenching Kit provides a dramatic reduction of autofluorescence, allowing a clear view of the desired signal, so it was utilized as needed on the specimens represented in the images. 

If co-labeling with a primary antibody, users should optimize each protocol separately to achieve maximum signal to noise ratios. Once this is complete, the two protocols can be combined, with the Glysite Scout Glycan Screening Kit protocol applied first, followed by the antibody staining protocol. Users should perform the proper controls to demonstrate that the reagents in each protocol are not cross–reacting with each other. 

The Carbo-Free Blocking Solution included in the Glysite Scout Glycan Screening Kit is virtually free of glycoproteins or other glycosylated molecules. This is critical since these kits detect glycans and would therefore bind to blocking reagents which contained glycosylated components. Users should not use other blocking solutions (e.g., milk derivatives, sera) as it may lead to high background. 

Each Glysite Scout Glycan Screening Kit contains enough reagents for approximately 50 tissue samples per lectin, or up to 400 samples if using one lectin per tissue sample (8 lectins x 50 tissue samples). 

The lectins were selected based upon their broad applicability and specificity to frequently cited glycan targets from literature sources, spanning fields of Cancer, Immunology, Metabolism, Inflammation, Virology, and Tumor Microenvironment, among others. They enable detection of all the major glycan motifs for the evaluation of glycan distribution in a target specimen. 

Technical Information

The lectins selected for the Glysite Scout Glycan Screening Kit, Immunofluorescence 488 were specifically chosen to provide detection of all of the major glycan motifs for the evaluation of glycan distribution in a target specimen. Follow our optimized protocol or view our how-to video to learn how you can rapidly explore glycan expression in your tissue section.

In addition to the glycan binders, the kit includes optimized immunofluorescence reagents for glycan detection, including blocking solutions, DyLight™ streptavidin, and VECTASHIELD Vibrance® Antifade Mounting Medium with DAPI.

Legal Information

DyLight is a trademark of Pierce Biotechnology, Inc.

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]]> Lycopersicon Esculentum (Tomato) Lectin (LEL, TL), DyLight® 488 https://staging.vectorlabs.com/products/dylight-488-lycopersicon-esculentum-tomato/ Tue, 25 Apr 2023 15:30:52 +0000 https://staging.vectorlabs.com/?post_type=product&p=11761 Description

Tomato lectin (from Lycopersicon esculentum) is an effective marker of blood vessels and microglial cells in rodents. Conjugation of the lectin with a fluorophore facilitates fast, one-step detection and visualization using intravascular perfusion methods or direct application to tissue sections.

DyLight™ 488 labeled tomato lectin has an appropriate number of fluorochromes bound to provide the optimum staining characteristics for this lectin. This conjugate is supplied essentially free of unconjugated fluorochromes. The excitation maximum is at 493 nm and the emission maximum is at 518 nm.

Specifications

Unit Size 1 mg
Applications Immunofluorescence, Glycobiology
Recommended Usage The recommended concentration range for use is 5-20 µg/ml. If a precipitate forms upon long-term storage, warm to 37 ºC.
Recommended Storage 2-8 °C
Maximum Excitation 493 nm
Maximum Emission 518 nm
Solution 10 mM HEPES, 0.15 M NaCl, pH 7.5, 0.08% sodium azide, 0.1 mM CaCl2.
Concentration 1 mg active conjugate/ml
Conjugate DyLight 488
Color of Fluorescence Green
Sugar Specificity [GlcNAc]1-3, N-Acetylglucosamine

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Description

Tomato lectin (from Lycopersicon esculentum) is an effective marker of blood vessels and microglial cells in rodents. Conjugation of the lectin with a fluorophore facilitates fast, one-step detection and visualization using intravascular perfusion methods or direct application to tissue sections.

DyLight™ 488 labeled tomato lectin has an appropriate number of fluorochromes bound to provide the optimum staining characteristics for this lectin. This conjugate is supplied essentially free of unconjugated fluorochromes. The excitation maximum is at 493 nm and the emission maximum is at 518 nm.

Specifications

Unit Size1 mg
ApplicationsImmunofluorescence, Glycobiology
Recommended UsageThe recommended concentration range for use is 5-20 µg/ml. If a precipitate forms upon long-term storage, warm to 37 ºC.
Recommended Storage2-8 °C
Maximum Excitation493 nm
Maximum Emission518 nm
Solution10 mM HEPES, 0.15 M NaCl, pH 7.5, 0.08% sodium azide, 0.1 mM CaCl2.
Concentration1 mg active conjugate/ml
ConjugateDyLight 488
Color of FluorescenceGreen
Sugar Specificity[GlcNAc]1-3, N-Acetylglucosamine

Documents

Product FAQs

Yes, there are a number of published references describing the use of TrueVIEW Autofluorescence Quenching Kit in the scientific literature. Please refer to a partial list of these publications in the Technical Information section of the product detail page for SP-8400.

Citations

Technical Information

Tomato lectin is a very stable single subunit glycoprotein containing about 50 percent arabinose and galactose and may form multimeric aggregates in solution. Tomato lectin, although sharing some specificities with potato lectin, Datura lectin, and wheat germ agglutinin, has been reported to be dissimilar in many respects. LEL binds well to glycophorin and Tamm-Horsfall glycoprotein and has been used effectively to label vascular endothelium in rodents.

Accompanying each fluorescent lectin is an analysis data sheet summarizing the results of our quality control tests and providing pertinent information on the product. All of these reagents are supplied as solutions preserved with sodium azide.

Inhibiting/Eluting Sugar: Chitin Hydrolysate

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]]> VectaFluor™ Excel Amplified Anti-Mouse IgG, DyLight® 488 Antibody Kit https://staging.vectorlabs.com/products/vectafluor-excel-dylight-488-anti-mouse-igg-kit/ Tue, 25 Apr 2023 15:17:28 +0000 https://staging.vectorlabs.com/?post_type=product&p=11745 Description

VectaFluor Excel Amplified Fluorescent Staining System offers a non-biotin amplification method for fluorescence applications. This system uses a ready-to-use (R.T.U.) Amplifier Antibody, followed by a ready-to-use (R.T.U.) VectaFluor DyLight dye-labeled detection system.

Features:

  • High sensitivity
  • Low background
  • VectaFluor DyLight 488 labeled anti-goat IgG antibody for detection
  • Excitation: 493 nm
  • Emission: 518 nm
  • Color: Green

Specifications

Unit Size 1 kit
Applications Immunofluorescence
Technology Amplified Fluorescence Systems
Conjugate DyLight 488
Color of Fluorescence Green
Target Species Mouse
Format Ready-to-Use

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Description

VectaFluor Excel Amplified Fluorescent Staining System offers a non-biotin amplification method for fluorescence applications. This system uses a ready-to-use (R.T.U.) Amplifier Antibody, followed by a ready-to-use (R.T.U.) VectaFluor DyLight dye-labeled detection system.

Features:

  • High sensitivity
  • Low background
  • VectaFluor DyLight 488 labeled anti-goat IgG antibody for detection
  • Excitation: 493 nm
  • Emission: 518 nm
  • Color: Green

Specifications

Unit Size1 kit
ApplicationsImmunofluorescence
TechnologyAmplified Fluorescence Systems
ConjugateDyLight 488
Color of FluorescenceGreen
Target SpeciesMouse
FormatReady-to-Use

Kit contents:

  • 15 ml 2.5% Normal Horse Serum for blocking
  • 15 ml Amplifier Antibody (Goat anti-mouse IgG)
  • 15 ml VectaFluor DyLight labeled Horse Anti-Goat IgG

Documents

Citations

Technical Information

All the reagents in the VectaFluor Excel Kit are affinity purified and extensively cross-adsorbed to ensure high sensitivity and low background. In addition, the VectaFluor Reagents are conjugated to the DyLight dyes using technology that ensures maximum labeling without compromising antibody affinity or specificity. DyLight dyes offer several advantages such as brighter fluorescence, greater photostability, and pH independence.

All reagents are supplied in stabilized, R.T.U. solutions for maximum convenience.

Kit Contents:

  • 15 ml 2.5% Normal Horse Serum (ready-to-use) for blocking
  • 15 ml Amplifier Antibody (Goat anti-mouse IgG; ready-to-use)
  • 15 ml VectaFluor DyLight labeled Horse Anti-Goat IgG (ready-to-use)

VectaFluorExcelDiagram jpg

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]]> VectaFluor™ Excel Amplified Anti-Rabbit IgG, DyLight™ 488 Antibody Kit https://staging.vectorlabs.com/products/vectafluor-excel-dylight-488-anti-rabbit-igg-kit/ Tue, 25 Apr 2023 14:57:30 +0000 https://staging.vectorlabs.com/?post_type=product&p=11720 Description

VectaFluor Excel Amplified DyLight™ 488 Anti-Rabbit IgG offers a non-biotin amplification method for fluorescence applications. This system uses a ready-to-use (R.T.U.) Amplifier Antibody, followed by a ready-to-use (R.T.U.) VectaFluor DyLight dye-labeled detection system.

Features:

  • High sensitivity
  • Low background
  • VectaFluor DyLight 488 labeled anti-goat IgG antibody for detection
  • Excitation: 493 nm
  • Emission: 518 nm
  • Color: Green

Specifications

Unit Size 1 kit
Applications Immunofluorescence
Technology Amplified Fluorescence Systems
Conjugate DyLight 488
Color of Fluorescence Green
Target Species Rabbit
Format Ready-to-Use

The post VectaFluor™ Excel Amplified Anti-Rabbit IgG, DyLight™ 488 Antibody Kit appeared first on VectorLabs.

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Description

VectaFluor Excel Amplified DyLight™ 488 Anti-Rabbit IgG offers a non-biotin amplification method for fluorescence applications. This system uses a ready-to-use (R.T.U.) Amplifier Antibody, followed by a ready-to-use (R.T.U.) VectaFluor DyLight dye-labeled detection system.

Features:

  • High sensitivity
  • Low background
  • VectaFluor DyLight 488 labeled anti-goat IgG antibody for detection
  • Excitation: 493 nm
  • Emission: 518 nm
  • Color: Green

Specifications

Unit Size1 kit
ApplicationsImmunofluorescence
TechnologyAmplified Fluorescence Systems
ConjugateDyLight 488
Color of FluorescenceGreen
Target SpeciesRabbit
FormatReady-to-Use

Kit contents:

  • 15 ml 2.5% Normal Horse Serum for blocking
  • 15 ml Amplifier Antibody (Goat anti-rabbit IgG)
  • 15 ml VectaFluor DyLight labeled Horse Anti-Goat IgG

Documents

Citations

Technical Information

All the reagents in the VectaFluor Excel Kit are affinity purified and extensively cross-adsorbed to ensure high sensitivity and low background. In addition, the VectaFluor™ Reagents are conjugated to the DyLight dyes using technology that ensures maximum labeling without compromising antibody affinity or specificity. DyLight dyes offer several advantages such as brighter fluorescence, greater photostability, and pH independence.

All reagents are supplied in stabilized, R.T.U. solutions for maximum convenience.

Kit Contents:

  • 15 ml 2.5% Normal Horse Serum (ready-to-use) for blocking
  • 15 ml Amplifier Antibody (Goat anti-rabbit IgG; ready-to-use)
  • 15 ml VectaFluor™ DyLight™ labeled Horse Anti-Goat IgG (ready-to-use)
 
  • Excitation maximum:  493 nm
  • Emission maximum:  518 nm
  • Color:  Green

VectaFluorExcelDiagram jpg

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]]> Horse Anti-Goat IgG Antibody (H+L), DyLight™ 488 https://staging.vectorlabs.com/products/dylight-488-horse-anti-goat-igg/ Tue, 25 Apr 2023 03:53:22 +0000 https://staging.vectorlabs.com/?post_type=product&p=11656 Description

DyLight 488 Horse Anti-Goat IgG Antibody can be used for immunofluorescence and other applications. Optimal F/P ratios have been established for each conjugate to ensure maximum fluorescence with minimal background staining.

Features:

  • Affinity-purified, ultrapure, high affinity antibody
  • Suitable for use with goat, sheep, and bovine IgG primary antibodies
  • Thoroughly adsorbed against serum and immunoglobulins from potentially interfering species
  • Recognizes both heavy and light chains (H+L)
  • Optimally labeled with DyLight™ 488 to provide the brightest label for fluorescence microscopy
  • Supplied in solution
  • Excitation: 493 nm
  • Emission: 518 nm
  • Color: Green

Specifications

Unit Size 1.5 mg
Applications Immunofluorescence, In situ hybridization, Blotting Applications, Flow Cytometry/Cell Separation
Concentration 1.5 mg active conjugate/ml
Recommended Storage 2-8 °C
Solution 10 mM HEPES, 0.15 M NaCl, pH 7.5, 0.08% sodium azide.
Maximum Emission 518 nm
Maximum Excitation 493 nm
Recommended Usage Recommended concentration range for use 5-20 µg/ml. If this antibody is to be used in tissues which may contain cross-reacting endogenous immunoglobulins, dilution of this antibody may be made in buffers containing 2% normal serum from the same species as the tissue.
Target Species Goat
Conjugate DyLight 488
Color of Fluorescence Green
Host Species Horse
Format Concentrate

Technical Information

The horse anti-goat Ig antibodies are prepared by hyperimmunizing animals in a manner that produces high affinity antibodies. These are then purified by an affinity chromatography procedure designed to remove any low affinity antibodies which may be present. Cross-reactivities that are likely to interfere with specific labeling are removed by solid-phase adsorption techniques. The final product is then subjected to rigorous quality control assays including immunodiffusion, solid-phase enzyme immunoassays, gel electrophoresis and solid-phase binding assays. In preparing the labeled antibodies, great care is taken to ensure the maximum degree of labeling with no alteration in the specificity and affinity of the antibody. The labeled antibody then undergoes a further series of quality control assays, including immunohistochemical analysis.

DyLight™ fluorescent dyes are direct alternatives to traditional fluorophores such as fluorescein (FITC) and rhodamine. The excitation and emission spectra parallel that of other commercially available fluorescent reagents allowing for easy substitution into an existing protocol without requiring any further instrumentation or filter sets.

DyLight™ dyes offer a number of potential advantages including greater photostability and brighter fluorescence.  DyLight™ dyes are completely stable over a pH range of 4-9 making them compatible with many aqueous-based buffers and diluents. The DyLight™ dyes can be applied as single labels or in combination with other DyLight™ dyes and fluorophores as part of a multiple immunofluorescent antigen staining methodology.  The DyLight™ dyes currently offered are DyLight™ 488 (green), DyLight™ 549 (orange), DyLight™ 594 (red), and DyLight™ 649 (far red).

Dylightspectra Jpg Dylightspectra Vectorlabs
Dylight Table1 Jpg Dylight-table1 Vectorlabs

The post Horse Anti-Goat IgG Antibody (H+L), DyLight™ 488 appeared first on VectorLabs.

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Description

DyLight 488 Horse Anti-Goat IgG Antibody can be used for immunofluorescence and other applications. Optimal F/P ratios have been established for each conjugate to ensure maximum fluorescence with minimal background staining.

Features:

  • Affinity-purified, ultrapure, high affinity antibody
  • Suitable for use with goat, sheep, and bovine IgG primary antibodies 
  • Thoroughly adsorbed against serum and immunoglobulins from potentially interfering species 
  • Recognizes both heavy and light chains (H+L) 
  • Optimally labeled with DyLight™ 488 to provide the brightest label for fluorescence microscopy 
  • Supplied in solution 
  • Excitation: 493 nm 
  • Emission: 518 nm 
  • Color: Green

Specifications

Unit Size1.5 mg
ApplicationsImmunofluorescence, In situ hybridization, Blotting Applications, Flow Cytometry/Cell Separation
Concentration1.5 mg active conjugate/ml
Recommended Storage2-8 °C
Solution10 mM HEPES, 0.15 M NaCl, pH 7.5, 0.08% sodium azide.
Maximum Emission518 nm
Maximum Excitation493 nm
Recommended UsageRecommended concentration range for use 5-20 µg/ml. If this antibody is to be used in tissues which may contain cross-reacting endogenous immunoglobulins, dilution of this antibody may be made in buffers containing 2% normal serum from the same species as the tissue.
Target SpeciesGoat
ConjugateDyLight 488
Color of FluorescenceGreen
Host SpeciesHorse
FormatConcentrate

Documents

Citations

Technical Information

The horse anti-goat Ig antibodies are prepared by hyperimmunizing animals in a manner that produces high affinity antibodies. These are then purified by an affinity chromatography procedure designed to remove any low affinity antibodies which may be present. Cross-reactivities that are likely to interfere with specific labeling are removed by solid-phase adsorption techniques. The final product is then subjected to rigorous quality control assays including immunodiffusion, solid-phase enzyme immunoassays, gel electrophoresis and solid-phase binding assays. In preparing the labeled antibodies, great care is taken to ensure the maximum degree of labeling with no alteration in the specificity and affinity of the antibody. The labeled antibody then undergoes a further series of quality control assays, including immunohistochemical analysis. 

DyLight™ fluorescent dyes are direct alternatives to traditional fluorophores such as fluorescein (FITC) and rhodamine. The excitation and emission spectra parallel that of other commercially available fluorescent reagents allowing for easy substitution into an existing protocol without requiring any further instrumentation or filter sets.  

DyLight™ dyes offer a number of potential advantages including greater photostability and brighter fluorescence.  DyLight™ dyes are completely stable over a pH range of 4-9 making them compatible with many aqueous-based buffers and diluents. The DyLight™ dyes can be applied as single labels or in combination with other DyLight™ dyes and fluorophores as part of a multiple immunofluorescent antigen staining methodology.  The DyLight™ dyes currently offered are DyLight™ 488 (green), DyLight™ 549 (orange), DyLight™ 594 (red), and DyLight™ 649 (far red).

DyLightspectra jpg
Dylight Table1 jpg

The post Horse Anti-Goat IgG Antibody (H+L), DyLight™ 488 appeared first on VectorLabs.

]]> Horse Anti-Mouse IgG Antibody (H+L), DyLight™ 488 https://staging.vectorlabs.com/products/dylight-488-horse-anti-mouse-igg/ Tue, 25 Apr 2023 03:19:01 +0000 https://staging.vectorlabs.com/?post_type=product&p=11587 Description

DyLight 488 Horse Anti-Mouse IgG Antibody can be used for immunofluorescence or other applications. Optimal F/P ratios have been established for each conjugate to ensure maximum fluorescence with minimal background staining.

Features:

  • Recognizes both heavy and light chains (H+L)
  • Supplied in solution
  • Excitation: 493 nm
  • Emission: 518 nm
  • Color: Green

Specifications

Unit Size 1.5 mg
Applications Immunofluorescence, In situ hybridization, Blotting Applications, Flow Cytometry/Cell Separation
Concentration 1.5 mg active conjugate/ml
Recommended Storage 2-8 °C
Solution 10 mM HEPES, 0.15 M NaCl, pH 7.5, 0.08% sodium azide.
Maximum Emission 518 nm
Maximum Excitation 493 nm
Recommended Usage Recommended concentration range for use 5-20 µg/ml. If this antibody is to be used in tissues which may contain cross-reacting endogenous immunoglobulins, dilution of this antibody may be made in buffers containing 2% normal serum from the same species as the tissue.
Target Species Mouse
Conjugate DyLight 488
Color of Fluorescence Green
Host Species Horse
Format Concentrate

Technical Information

The anti-mouse Ig antibodies are prepared by hyperimmunizing animals in a manner that produces high affinity antibodies. These are then purified by an affinity chromatography procedure designed to remove any low affinity antibodies which may be present. Cross-reactivities that are likely to interfere with specific labeling are removed by solid-phase adsorption techniques. The final product is then subjected to rigorous quality control assays including immunodiffusion, solid-phase enzyme immunoassays, gel electrophoresis and solid-phase binding assays. In preparing the labeled antibodies, great care is taken to ensure the maximum degree of labeling with no alteration in the specificity and affinity of the antibody. The labeled antibody then undergoes a further series of quality control assays, including immunohistochemical analysis.

DyLight™ fluorescent dyes are direct alternatives to traditional fluorophores such as fluorescein (FITC) and rhodamine. The excitation and emission spectra parallel that of other commercially available fluorescent reagents allowing for easy substitution into an existing protocol without requiring any further instrumentation or filter sets.

DyLight™ dyes offer a number of potential advantages including greater photostability and brighter fluorescence.  DyLight™ dyes are completely stable over a pH range of 4-9 making them compatible with many aqueous-based buffers and diluents. The DyLight™ dyes can be applied as single labels or in combination with other DyLight™ dyes and fluorophores as part of a multiple immunofluorescent antigen staining methodology.  The DyLight™ dyes currently offered are DyLight™ 488 (green), DyLight™ 549 (orange), DyLight™ 594 (red), and DyLight™ 649 (far red).

Dylightspectra Jpg Dylightspectra Vectorlabs
Dylight Table1 Jpg Dylight-table1 Vectorlabs

The post Horse Anti-Mouse IgG Antibody (H+L), DyLight™ 488 appeared first on VectorLabs.

]]>

Description

DyLight 488 Horse Anti-Mouse IgG Antibody can be used for immunofluorescence or other applications. Optimal F/P ratios have been established for each conjugate to ensure maximum fluorescence with minimal background staining.

Features:

  • Recognizes both heavy and light chains (H+L) 
  • Supplied in solution 
  • Excitation: 493 nm 
  • Emission: 518 nm 
  • Color: Green

Specifications

Unit Size1.5 mg
ApplicationsImmunofluorescence, In situ hybridization, Blotting Applications, Flow Cytometry/Cell Separation
Concentration1.5 mg active conjugate/ml
Recommended Storage2-8 °C
Solution10 mM HEPES, 0.15 M NaCl, pH 7.5, 0.08% sodium azide.
Maximum Emission518 nm
Maximum Excitation493 nm
Recommended UsageRecommended concentration range for use 5-20 µg/ml. If this antibody is to be used in tissues which may contain cross-reacting endogenous immunoglobulins, dilution of this antibody may be made in buffers containing 2% normal serum from the same species as the tissue.
Target SpeciesMouse
ConjugateDyLight 488
Color of FluorescenceGreen
Host SpeciesHorse
FormatConcentrate

Documents

Citations

Technical Information

The anti-mouse Ig antibodies are prepared by hyperimmunizing animals in a manner that produces high affinity antibodies. These are then purified by an affinity chromatography procedure designed to remove any low affinity antibodies which may be present. Cross-reactivities that are likely to interfere with specific labeling are removed by solid-phase adsorption techniques. The final product is then subjected to rigorous quality control assays including immunodiffusion, solid-phase enzyme immunoassays, gel electrophoresis and solid-phase binding assays. In preparing the labeled antibodies, great care is taken to ensure the maximum degree of labeling with no alteration in the specificity and affinity of the antibody. The labeled antibody then undergoes a further series of quality control assays, including immunohistochemical analysis. 

DyLight™ fluorescent dyes are direct alternatives to traditional fluorophores such as fluorescein (FITC) and rhodamine. The excitation and emission spectra parallel that of other commercially available fluorescent reagents allowing for easy substitution into an existing protocol without requiring any further instrumentation or filter sets.  

DyLight™ dyes offer a number of potential advantages including greater photostability and brighter fluorescence.  DyLight™ dyes are completely stable over a pH range of 4-9 making them compatible with many aqueous-based buffers and diluents. The DyLight™ dyes can be applied as single labels or in combination with other DyLight™ dyes and fluorophores as part of a multiple immunofluorescent antigen staining methodology.  The DyLight™ dyes currently offered are DyLight™ 488 (green), DyLight™ 549 (orange), DyLight™ 594 (red), and DyLight™ 649 (far red).

DyLightspectra jpg
Dylight Table1 jpg

The post Horse Anti-Mouse IgG Antibody (H+L), DyLight™ 488 appeared first on VectorLabs.

]]> VectaFluor™ Horse Anti-Rabbit IgG, DyLight™ 488 Antibody Kit, R.T.U. https://staging.vectorlabs.com/products/vectafluor-dylightr-488-anti-rabbit-igg/ Tue, 25 Apr 2023 03:06:35 +0000 https://staging.vectorlabs.com/?post_type=product&p=11569 Description

VectaFluor R.T.U. DyLight 488 Anti-Rabbit IgG can be used for immunofluorescence and other applications. Optimal F/P ratios have been established for each conjugate to ensure maximum fluorescence with minimal background staining.

Features:

  • Ready-to-use format offers maximum convenience for fluorescence staining of cells and tissues
  • Recognizes both heavy and light chains (H+L)
  • Optimally labeled with DyLight 488 to provide the brightest label for fluorescence microscopy
  • Excitation: 493 nm
  • Emission: 518 nm
  • Color: Green

Kit Contents:

  • 15 ml pre-diluted, stabilized secondary antibody solution
  • 15 ml 2.5% normal horse serum for blocking

Specifications

Unit Size 1 kit
Applications Immunofluorescence, In situ hybridization, Flow Cytometry/Cell Separation
Maximum Emission 518 nm
Maximum Excitation 493 nm
Target Species Rabbit
Conjugate DyLight 488
Color of Fluorescence Green
Host Species Horse
Format Ready-to-Use

Technical Information

VectaFluor Ready-to-Use (R.T.U.) DyLight dye-labeled Secondary Antibodies offer maximum convenience for fluorescence staining of cells and tissues. Our affinity purified, extensively cross-adsorbed secondary antibodies are conjugated to DyLight dyes in a manner that ensures maximum degree of labeling without compromising antibody affinity or specificity.

VectaFluor R.T.U. Antibody Reagents are supplied as 15 ml pre-diluted, stabilized, ready-to-use solutions along with 15 ml ready-to-use 2.5% normal horse serum for blocking.

  • Excitation maximum:   493 nm
  • Emission maximum:  518 nm
  • Color:  Green

 

DyLight fluorescent dyes are direct alternatives to traditional fluorophores such as fluorescein (FITC) and rhodamine. The excitation and emission spectra parallel that of other commercially available fluorescent reagents allowing for easy substitution into an existing protocol without requiring any further instrumentation or filter sets.

DyLight dyes offer a number of potential advantages including greater photostability and brighter fluorescence.  DyLight dyes are completely stable over a pH range of 4-9 making them compatible with many aqueous-based buffers and diluents. The DyLight dyes can be applied as single labels or in combination with other DyLight dyes and fluorophores as part of a multiple immunofluorescent antigen staining methodology.  The Dylight dyes currently offered are DyLight 488 (green), DyLight 549 (orange), DyLight 594 (red), and DyLight 649 (far red).

Dylightspectra Jpg Dylightspectra Vectorlabs
Dylight Table1 Jpg Dylight-table1 Vectorlabs

The post VectaFluor™ Horse Anti-Rabbit IgG, DyLight™ 488 Antibody Kit, R.T.U. appeared first on VectorLabs.

]]>

Description

VectaFluor R.T.U. DyLight 488 Anti-Rabbit IgG can be used for immunofluorescence and other applications. Optimal F/P ratios have been established for each conjugate to ensure maximum fluorescence with minimal background staining.

Features:

  • Ready-to-use format offers maximum convenience for fluorescence staining of cells and tissues
  • Recognizes both heavy and light chains (H+L) 
  • Optimally labeled with DyLight 488 to provide the brightest label for fluorescence microscopy 
  • Excitation: 493 nm 
  • Emission: 518 nm 
  • Color: Green

 

Kit Contents:

  • 15 ml pre-diluted, stabilized secondary antibody solution 
  • 15 ml 2.5% normal horse serum for blocking

Specifications

Unit Size1 kit
ApplicationsImmunofluorescence, In situ hybridization, Flow Cytometry/Cell Separation
Maximum Emission518 nm
Maximum Excitation493 nm
Target SpeciesRabbit
ConjugateDyLight 488
Color of FluorescenceGreen
Host SpeciesHorse
FormatReady-to-Use

Documents

Citations

Technical Information

VectaFluor Ready-to-Use (R.T.U.) DyLight dye-labeled Secondary Antibodies offer maximum convenience for fluorescence staining of cells and tissues. Our affinity purified, extensively cross-adsorbed secondary antibodies are conjugated to DyLight dyes in a manner that ensures maximum degree of labeling without compromising antibody affinity or specificity. 

VectaFluor R.T.U. Antibody Reagents are supplied as 15 ml pre-diluted, stabilized, ready-to-use solutions along with 15 ml ready-to-use 2.5% normal horse serum for blocking.

  • Excitation maximum:   493 nm
  • Emission maximum:  518 nm
  • Color:  Green

DyLight fluorescent dyes are direct alternatives to traditional fluorophores such as fluorescein (FITC) and rhodamine. The excitation and emission spectra parallel that of other commercially available fluorescent reagents allowing for easy substitution into an existing protocol without requiring any further instrumentation or filter sets.  

DyLight dyes offer a number of potential advantages including greater photostability and brighter fluorescence.  DyLight dyes are completely stable over a pH range of 4-9 making them compatible with many aqueous-based buffers and diluents. The DyLight dyes can be applied as single labels or in combination with other DyLight dyes and fluorophores as part of a multiple immunofluorescent antigen staining methodology.  The Dylight dyes currently offered are DyLight 488 (green), DyLight 549 (orange), DyLight 594 (red), and DyLight 649 (far red).

DyLightspectra jpg
Dylight Table1 jpg

The post VectaFluor™ Horse Anti-Rabbit IgG, DyLight™ 488 Antibody Kit, R.T.U. appeared first on VectorLabs.

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