Streptavidin – VectorLabs https://staging.vectorlabs.com From linker design and synthesis, bioconjugated design and manufacturing, biomolecule labeling and functionalization, and detection system and solutions, our team is here to help you move forward, with impact. Wed, 22 Oct 2025 17:02:46 +0000 en-US hourly 1 https://wordpress.org/?v=6.9 https://staging.vectorlabs.com/wp-content/uploads/2024/11/V-favicon-02-100x100.png Streptavidin – VectorLabs https://staging.vectorlabs.com 32 32 Sulfo ChromaLINK® Biotin (Water Soluble) https://staging.vectorlabs.com/products/chromalink-biotin-antibody-labeling-h20sol/ Wed, 03 May 2023 04:13:19 +0000 https://staging.vectorlabs.com/?post_type=product&p=14796 Description

ChromaLINK Biotin contains a UV-traceable chromophore based on ChromaLINK technology to enable reproducibility in your biotinylation process. Now you can measure the degree of biotinylation in minutes, not hours, without the standard curves required for HABA/avidin and fluoro-reporter assays. With a simple, non-destructive UV scan, you can quantify biotin incorporation and ensure reproducible production of consistent batches. This biotinylation reagent is water soluble, which means no DMF or other organic solvent is required to dissolve the compound prior to labeling.

Specifications

Label/Modifier Type biotin
Reactivity Streptavidin
Recommended Storage Desiccated: -15° to -25°C
Applications Antibody Labeling, Aptamers
Other Name(s) SoluLINK Bioconjugation

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Description

ChromaLINK Biotin contains a UV-traceable chromophore based on ChromaLINK technology to enable reproducibility in your biotinylation process. Now you can measure the degree of biotinylation in minutes, not hours, without the standard curves required for HABA/avidin and fluoro-reporter assays. With a simple, non-destructive UV scan, you can quantify biotin incorporation and ensure reproducible production of consistent batches. This biotinylation reagent is water soluble, which means no DMF or other organic solvent is required to dissolve the compound prior to labeling.

Specifications

Label/Modifier Typebiotin
ReactivityStreptavidin
Recommended StorageDesiccated: -15° to -25°C
ApplicationsAntibody Labeling, Aptamers
Other Name(s)SoluLINK Bioconjugation

Documents

Citations

Technical Information

Introduction to ChromaLINK Labeling Technology

ChromaLINK Biotin incorporates UV-traceable biotin onto proteins containing lysine residues (amine groups) via a water-soluble-succinimidyl activated ester. ChromaLINK Biotin has been engineered to include many novel features. As illustrated in Figure 1, the molecule’s structure contains a bis-aryl hydrazone chromophore (a), linked by a PEG3 linker arm (b), to biotin (c). This reagent permits direct spectroscopic quantification of incorporated biotin. The extended PEG3 linker preserves biotin/streptavidin affinity and maintains protein solubility after modification while the succinimidyl ester functional group (d), efficiently modifies lysines in aqueous buffers.

B 1007 F1

Figure 1. Molecular structure of ChromaLINK Biotin (water soluble)

Labeling of proteins with ChromaLINK Biotin eliminates the need to carry out cumbersome and time-consuming HABA assays often employed to quantify biotin incorporation. Instead, biotin incorporation is quantified by means of a simple spectrophotometric measurement at two wavelengths (A280 / A354). Typical labeling results are illustrated in Figure 2 by spectral overlay scans of four samples. As illustrated, bovine IgG (100 ul @ 5 mg/ml) was labeled at 0, 5,10, and 15 mole equivalents using ChromaLINK Biotin. Spectral analysis illustrates how easy it is to visualize, confirm, and quantify biotin incorporation.

B 1007 F2 jpg

Figure 2. Superimposed spectra of bovine IgG biotinylated using ChromaLINK Biotin. Various biotin-to-protein mole equivalents (5X, 10X and 15X) were used. Note the traceable UV-signature @ 354nm indicating incorporation of biotin. All spectra were scanned on a Molecular Dynamics SpectraMax PlusTM UV-VIS plate reader (220-420 nm).

Legal Information

SoluLINK® Bioconjugation For Research Use Only. Not for use in diagnostic procedures. For additional licensing restrictions, please see the license agreement at vectorlabs.com/solulink-research-license.

Products are for research use only, not for use in diagnostic or therapeutic procedures or for use in humans. Products are not for resale without express written permission of Seller. No license under any patent or other intellectual property right of Seller or its licensors is granted or implied by the purchase unless otherwise provided in writing.

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]]> Anti-Streptavidin Antibody, DyLight® 488 https://staging.vectorlabs.com/products/dylight-488-anti-streptavidin/ Tue, 02 May 2023 02:35:55 +0000 https://staging.vectorlabs.com/?post_type=product&p=13944 Description

Anti-Streptavidin antibodies have been widely used as amplifying reagents in immunohistochemistry, in situ hybridization, microarray assays, ELISAs, blots, and many other applications. Our antibodies to streptavidin are produced in goats using our highly purified streptavidin and isolated by affinity chromatography. Anti-Streptavidin does not bind avidin and Anti-Avidin does not recognize streptavidin. These antibodies provide opportunities to significantly amplify signals in many applications.

DyLight 488 Anti-Streptavidin has been optimally labeled to provide maximum fluorescence.

  • Maximum Excitation: 493 nm
  • Maximum Emission: 518 nm
  • Color: green

Specifications

Unit Size 0.5 mg
Applications Immunofluorescence, In situ hybridization
Concentration 1 mg active conjugate/ml
Recommended Storage 2-8 °C
Solution 10 mM HEPES, pH 7.5, 0.15 M NaCl, 0.08% sodium azide
Recommended Usage The recommended concentration range for use is 5-20 µg/ml.
Detection Target Streptavidin
Conjugate DyLight 488
Color of Fluorescence Green
Host Species Goat
Format Concentrate

The post Anti-Streptavidin Antibody, DyLight® 488 appeared first on VectorLabs.

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Description

Anti-Streptavidin antibodies have been widely used as amplifying reagents in immunohistochemistry, in situ hybridization, microarray assays, ELISAs, blots, and many other applications. Our antibodies to streptavidin are produced in goats using our highly purified streptavidin and isolated by affinity chromatography. Anti-Streptavidin does not bind avidin and Anti-Avidin does not recognize streptavidin. These antibodies provide opportunities to significantly amplify signals in many applications.

DyLight 488 Anti-Streptavidin has been optimally labeled to provide maximum fluorescence.

  • Maximum Excitation: 493 nm
  • Maximum Emission: 518 nm
  • Color: green

Specifications

Unit Size0.5 mg
ApplicationsImmunofluorescence, In situ hybridization
Concentration1 mg active conjugate/ml
Recommended Storage2-8 °C
Solution10 mM HEPES, pH 7.5, 0.15 M NaCl, 0.08% sodium azide
Recommended UsageThe recommended concentration range for use is 5-20 µg/ml.
Detection TargetStreptavidin
ConjugateDyLight 488
Color of FluorescenceGreen
Host SpeciesGoat
FormatConcentrate

Documents

Citations

Technical Information

The capability of binding avidin or streptavidin via either biotin binding sites or through antigen binding sites, makes these biotinylated antibodies unique. These antibodies can be used either as part of preformed complexes or in sequence to amplify fluorescent signals. When used in sequence, the target is first labeled with fluorochrome-conjugated avidin or streptavidin, followed by incubation with Biotinylated Anti-Avidin or Biotinylated Anti-Streptavidin, followed by a second layer of fluorochrome-conjugated avidin or streptavidin. This sequence can be repeated. This multi-layered approach introduces more fluorochromes at the target site and can provide a multi-fold amplification over a single layer.

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]]> Anti-Streptavidin Antibody, Unconjugated https://staging.vectorlabs.com/products/unconjugated-anti-streptavidin/ Tue, 02 May 2023 01:14:39 +0000 https://staging.vectorlabs.com/?post_type=product&p=13826 Description

Anti-Streptavidin antibodies have been widely used as amplifying reagents in immunohistochemistry, in situ hybridization, microarray assays, ELISAs, blots, and many other applications.  Our antibodies to streptavidin are produced in goats using our highly purified streptavidin and isolated by affinity chromatography. Anti-Streptavidin does not bind avidin and Anti-Avidin does not recognize streptavidin. These antibodies provide opportunities to significantly amplify signals in many applications.

Specifications

More Information
Unit Size 1 mg
Applications Immunofluorescence, In situ hybridization
Concentration 2 mg active IgG/ml
Recommended Storage 2-8 °C; Store frozen for long term storage
Solution 10 mM HEPES, pH 7.5, 0.15 M NaCl, 0.08% sodium azide, 20 mg/ml sucrose.
Recommended Usage The recommended concentration range for use is 2-5 µg/ml.
Detection Target Streptavidin
Conjugate Unconjugated
Host Species Goat
Format Concentrate

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Description

Anti-Streptavidin antibodies have been widely used as amplifying reagents in immunohistochemistry, in situ hybridization, microarray assays, ELISAs, blots, and many other applications.  Our antibodies to streptavidin are produced in goats using our highly purified streptavidin and isolated by affinity chromatography. Anti-Streptavidin does not bind avidin and Anti-Avidin does not recognize streptavidin. These antibodies provide opportunities to significantly amplify signals in many applications.

Specifications

More Information
Unit Size1 mg
ApplicationsImmunofluorescence, In situ hybridization
Concentration2 mg active IgG/ml
Recommended Storage2-8 °C; Store frozen for long term storage
Solution10 mM HEPES, pH 7.5, 0.15 M NaCl, 0.08% sodium azide, 20 mg/ml sucrose.
Recommended UsageThe recommended concentration range for use is 2-5 µg/ml.
Detection TargetStreptavidin
ConjugateUnconjugated
Host SpeciesGoat
FormatConcentrate

Documents

 

Citations

Technical Information

These affinity purified antibodies are also available biotin or fluorescein-labeled.

The post Anti-Streptavidin Antibody, Unconjugated appeared first on VectorLabs.

]]> Goat Anti-Streptavidin Antibody, Biotinylated https://staging.vectorlabs.com/products/anti-streptavidin-antibody-biotinylated/ Mon, 24 Apr 2023 15:26:31 +0000 https://staging.vectorlabs.com/?post_type=product&p=11156 Description

Biotinylated Anti-Streptavidin has been widely used as an amplifying reagent in immunohistochemistry, in situ hybridization, microarray assays, ELISAs, blots, and many other applications. The capability of binding streptavidin via either biotin binding sites or through antigen binding sites, makes this biotinylated antibody unique. Anti-Streptavidin does not bind avidin, and Anti-Avidin does not recognize streptavidin.

Specifications

Unit Size 0.5 mg
Applications Immunofluorescence, In situ hybridization
Concentration 0.5 mg active conjugate/ml
Recommended Storage 2-8 °C; Store frozen for long term storage
Solution 10 mM HEPES, pH 7.5, 0.15 M NaCl, 0.08% sodium azide, 20 mg/ml sucrose.
Recommended Usage The recommended concentration range for use is 1-10 µg/ml. If this biotinylated antibody is to be used in tissues, which may contain cross-reacting endogenous immunoglobulins, dilution of this biotinylated antibody may be made in buffers containing 2% normal serum from the same species as the tissue.
Detection Target Streptavidin
Conjugate Biotinylated
Host Species Goat
Format Concentrate

Technical Information

These antibodies can be used either as part of preformed complexes or in sequence to amplify fluorescent signals. When used in sequence, the target is first labeled with fluorochrome-conjugated avidin or streptavidin, followed by incubation with Biotinylated Anti-Avidin or Biotinylated Anti-Streptavidin, followed by a second layer of fluorochrome-conjugated avidin or streptavidin. This sequence can be repeated. This multi-layered approach introduces more fluorochromes at the target site and can provide a multi-fold amplification over a single layer.

These affinity purified antibodies are also available unconjugated or fluorescein-labeled.

The following figures illustrate different experimental setups used to achieve increasing levels of sensitivity.

B2 Fig1 3 800 Jpg B2-fig1-3-800 Vectorlabs

The post Goat Anti-Streptavidin Antibody, Biotinylated appeared first on VectorLabs.

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Description

Biotinylated Anti-Streptavidin has been widely used as an amplifying reagent in immunohistochemistry, in situ hybridization, microarray assays, ELISAs, blots, and many other applications. The capability of binding streptavidin via either biotin binding sites or through antigen binding sites, makes this biotinylated antibody unique. Anti-Streptavidin does not bind avidin, and Anti-Avidin does not recognize streptavidin. 

Specifications

Unit Size0.5 mg
ApplicationsImmunofluorescence, In situ hybridization
Concentration0.5 mg active conjugate/ml
Recommended Storage2-8 °C; Store frozen for long term storage
Solution10 mM HEPES, pH 7.5, 0.15 M NaCl, 0.08% sodium azide, 20 mg/ml sucrose.
Recommended UsageThe recommended concentration range for use is 1-10 µg/ml. If this biotinylated antibody is to be used in tissues, which may contain cross-reacting endogenous immunoglobulins, dilution of this biotinylated antibody may be made in buffers containing 2% normal serum from the same species as the tissue.
Detection TargetStreptavidin
ConjugateBiotinylated
Host SpeciesGoat
FormatConcentrate

Documents

Citations

Technical Information

These antibodies can be used either as part of preformed complexes or in sequence to amplify fluorescent signals. When used in sequence, the target is first labeled with fluorochrome-conjugated avidin or streptavidin, followed by incubation with Biotinylated Anti-Avidin or Biotinylated Anti-Streptavidin, followed by a second layer of fluorochrome-conjugated avidin or streptavidin. This sequence can be repeated. This multi-layered approach introduces more fluorochromes at the target site and can provide a multi-fold amplification over a single layer.

These affinity purified antibodies are also available unconjugated or fluorescein-labeled.

The following figures illustrate different experimental setups used to achieve increasing levels of sensitivity.

B2 Fig1 3 800 jpg

The post Goat Anti-Streptavidin Antibody, Biotinylated appeared first on VectorLabs.

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