7 EO – VectorLabs https://staging.vectorlabs.com From linker design and synthesis, bioconjugated design and manufacturing, biomolecule labeling and functionalization, and detection system and solutions, our team is here to help you move forward, with impact. Wed, 22 Oct 2025 17:19:59 +0000 en-US hourly 1 https://wordpress.org/?v=6.9 https://staging.vectorlabs.com/wp-content/uploads/2024/11/V-favicon-02-100x100.png 7 EO – VectorLabs https://staging.vectorlabs.com 32 32 Bis-dPEG®₇-NHS ester https://staging.vectorlabs.com/products/bis-dpeg7-nhs-ester/ Sat, 24 Feb 2024 02:14:35 +0000 https://staging.vectorlabs.com/?post_type=product&p=37294 Bis-dPEG®7-NHS ester, product number QBD-10988, is a homobifunctional, amine-reactive, single molecular weight PEG crosslinker with a short (25 atoms, 28.6 Å) discrete PEG (dPEG®) chain length. Each end of the chain terminates as the N-hydroxysuccinimidyl (NHS) ester of propionic acid. Unlike traditional hydrophobic crosslinkers, Bis-dPEG®7-NHS ester will not cause problems such as aggregation, precipitation, and inactivation when conjugated to biomolecules. As a homobifunctional, amine-reactive crosslinker, the primary uses for QBD-10988 are intermolecular conjugation of biomolecules with free, surface-accessible amines and intramolecular modification of biomolecules using free, surface-accessible amines. Because QBD-10988 is one in a line of similar products, Bis-dPEG®7-NHS ester can be used with other products in the same line to probe the effect of different linker lengths on the performance of crosslinked or intramolecularly modified biomolecules.

NHS esters react with free amines such as the ε-amines of lysine. The optimal pH range for NHS esters to react with free amines is 7.0 – 7.5. However, NHS esters can react with free amines with pH as low as 6.5. NHS esters are susceptible to hydrolysis in aqueous media. As the pH increases, the hydrolysis rate of the ester increases. Thus, we strongly discourage storing Bis-dPEG®7-NHS ester in water or aqueous buffer. Instead, we recommend that customers make new solutions of the product as needed, use them immediately, and discard unused solutions after use. If customers desire to store the product in solution, we recommend the use of a pure, anhydrous, water-miscible solvent such as dimethyl sulfoxide (DMSO), N,N-dimethylacetamide (DMAC), or N,N-dimethylformamide (DMF). DMSO, DMAC, or DMF can be dried chemically or by storing for a minimum of 24 hours over 3 Å molecular sieves. With DMF, use only fresh solvent as the compound decomposes over time to form free amines that will react with the NHS esters.

Specifications

Unit Size 100mg, 1000mg
Molecular Weight 620.60; single compound
Chemical formula C₂₆H₄₀N₂O₁₅
CAS 1334170-02-3
Purity > 98%
Spacers dPEG® Spacer is 25 atoms and 28.6 Å
Shipping Ambient
Typical solubility properties (for additional information contact Customer Support) Methylene chloride, Acetonitrile, DMAC or DMSO.
Storage and handling -20°C; Always let come to room temperature before opening; be careful to limit exposure to moisture and restore under an inert atmosphere; stock solutions can be prepared with dry solvent and kept for several days (freeze when not in use). dPEG® pegylation compounds are generally hygroscopic and should be treated as such. This will be less noticeable with liquids, but the solids will become tacky and difficult to manipulate, if care is not taken to minimize air exposure.

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Description

Bis-dPEG®7-NHS ester, product number QBD-10988, is a homobifunctional, amine-reactive, single molecular weight PEG crosslinker with a short (25 atoms, 28.6 Å) discrete PEG (dPEG®) chain length. Each end of the chain terminates as the N-hydroxysuccinimidyl (NHS) ester of propionic acid. Unlike traditional hydrophobic crosslinkers, Bis-dPEG®7-NHS ester will not cause problems such as aggregation, precipitation, and inactivation when conjugated to biomolecules. As a homobifunctional, amine-reactive crosslinker, the primary uses for QBD-10988 are intermolecular conjugation of biomolecules with free, surface-accessible amines and intramolecular modification of biomolecules using free, surface-accessible amines. Because QBD-10988 is one in a line of similar products, Bis-dPEG®7-NHS ester can be used with other products in the same line to probe the effect of different linker lengths on the performance of crosslinked or intramolecularly modified biomolecules.

NHS esters react with free amines such as the ε-amines of lysine. The optimal pH range for NHS esters to react with free amines is 7.0 – 7.5. However, NHS esters can react with free amines with pH as low as 6.5. NHS esters are susceptible to hydrolysis in aqueous media. As the pH increases, the hydrolysis rate of the ester increases. Thus, we strongly discourage storing Bis-dPEG®7-NHS ester in water or aqueous buffer. Instead, we recommend that customers make new solutions of the product as needed, use them immediately, and discard unused solutions after use. If customers desire to store the product in solution, we recommend the use of a pure, anhydrous, water-miscible solvent such as dimethyl sulfoxide (DMSO), N,N-dimethylacetamide (DMAC), or N,N-dimethylformamide (DMF). DMSO, DMAC, or DMF can be dried chemically or by storing for a minimum of 24 hours over 3 Å molecular sieves. With DMF, use only fresh solvent as the compound decomposes over time to form free amines that will react with the NHS esters.

Specifications

Unit Size100mg, 1000mg
Molecular Weight620.60; single compound
Chemical formulaC₂₆H₄₀N₂O₁₅
CAS1334170-02-3
Purity> 98%
SpacersdPEG® Spacer is 25 atoms and 28.6 Å
ShippingAmbient
Typical solubility properties (for additional information contact Customer Support)Methylene chloride, Acetonitrile, DMAC or DMSO.
Storage and handling-20°C; Always let come to room temperature before opening; be careful to limit exposure to moisture and restore under an inert atmosphere; stock solutions can be prepared with dry solvent and kept for several days (freeze when not in use). dPEG® pegylation compounds are generally hygroscopic and should be treated as such. This will be less noticeable with liquids, but the solids will become tacky and difficult to manipulate, if care is not taken to minimize air exposure.



Documents

References

Inhibition of HLA-DQ2 Mediated Antigen Presentation by Analogues of a High Affinity 33-Residue Peptide from α2-Gliadin. Jiang Xia, Matthew Siegel, Elin Bergseng, Ludvig M. Sollid, and Chaitan Khosla. Journal of the American Chemical Society 2006 128 (6) pp 1859-1867 February 15, 2006. DOI:10.1021/ja056423o.

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]]> Azido-dPEG®₇-amine https://staging.vectorlabs.com/products/azido-dpeg7-amine/ Sat, 24 Feb 2024 02:11:18 +0000 https://staging.vectorlabs.com/?post_type=product&p=36305 Azido-dPEG®7-amine, product number QBD-10523, is a carbonyl-reactive, biocompatible crosslinking reagent that can participate in multiple types of reactions. The product consists of an azide group and an amine group on opposite ends of a single molecular weight, discrete polyethylene glycol (dPEG®) chain. Alkynes react with the azido group in metal-catalyzed or strain-promoted click chemistry to yield triazole derivatives. Moreover, the azide group functions as a masked amine that reduces to a primary amine via a suitable reducing agent. Furthermore, Azido-dPEG®7-amine can participate in a Staudinger ligation with appropriate phosphine derivatives to create a water-soluble product.

The amphiphilic dPEG® product adds hydrodynamic volume and imparts water solubility to any compound to which it is conjugated. Also, because the terminal azide group functions in multiple different types of reactions, Azido-dPEG®7-amine can act as either a heterobifunctional, click chemistry crosslinker or a monoprotected homobifunctional crosslinking reagent.

Specifications

Unit Size 100 mg, 1000 mg
Molecular Weight 394.46; single compound
Chemical formula C₁₆H₃₄N₄O₇
CAS 1333154-77-0
Purity > 98%
Spacers dPEG® Spacer is 25 atoms and 28.8 Å
Shipping Ambient
Typical solubility properties (for additional information contact Customer Support) Methylene chloride, Acetonitrile, DMAC, or DMSO.
Storage and handling -20°C; Always let come to room temperature before opening; be careful to limit exposure to moisture and restore under an inert atmosphere; stock solutions can be prepared with dry solvent and kept for several days (freeze when not in use). dPEG® pegylation compounds are generally hygroscopic and should be treated as such. This will be less noticeable with liquids, but the solids will become tacky and difficult to manipulate, if care is not taken to minimize air exposure.

The post Azido-dPEG®₇-amine appeared first on VectorLabs.

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Description

Azido-dPEG®7-amine, product number QBD-10523, is a carbonyl-reactive, biocompatible crosslinking reagent that can participate in multiple types of reactions. The product consists of an azide group and an amine group on opposite ends of a single molecular weight, discrete polyethylene glycol (dPEG®) chain. Alkynes react with the azido group in metal-catalyzed or strain-promoted click chemistry to yield triazole derivatives. Moreover, the azide group functions as a masked amine that reduces to a primary amine via a suitable reducing agent. Furthermore, Azido-dPEG®7-amine can participate in a Staudinger ligation with appropriate phosphine derivatives to create a water-soluble product.

The amphiphilic dPEG® product adds hydrodynamic volume and imparts water solubility to any compound to which it is conjugated. Also, because the terminal azide group functions in multiple different types of reactions, Azido-dPEG®7-amine can act as either a heterobifunctional, click chemistry crosslinker or a monoprotected homobifunctional crosslinking reagent.

Specifications

Unit Size100 mg, 1000 mg
Molecular Weight394.46; single compound
Chemical formulaC₁₆H₃₄N₄O₇
CAS1333154-77-0
Purity> 98%
SpacersdPEG® Spacer is 25 atoms and 28.8 Å
ShippingAmbient
Typical solubility properties (for additional information contact Customer Support)Methylene chloride, Acetonitrile, DMAC, or DMSO.
Storage and handling-20°C; Always let come to room temperature before opening; be careful to limit exposure to moisture and restore under an inert atmosphere; stock solutions can be prepared with dry solvent and kept for several days (freeze when not in use). dPEG® pegylation compounds are generally hygroscopic and should be treated as such. This will be less noticeable with liquids, but the solids will become tacky and difficult to manipulate, if care is not taken to minimize air exposure.



Documents

References

Greg T. Hermanson, Bioconjugate Techniques, 3rd Edition, Elsevier, Waltham, MA 02451, 2013, ISBN 978-0-12-382239-0; See Chapter 18, Discrete PEG Reagents, pp. 787-821, for a full overview of the dPEG® products.

Tuning the Properties of Layer-by-Layer Assembled Poly(acrylic acid) Click Films and Capsules. Cameron R. Kinnane, Georgina K. Such, and Frank Caruso. Macromolecules, 2011, 44 (5), pp 1194–1202 February 4, 2010. DOI: 10.1021/ma102593k.

Detection of SK2 Channels on Hippocampal Neurons. Jamie L. Maciaszek University of Connecticut (2012) DigitalCommons@Uconn Master’s Theses Paper 237 April 20, 2012. http://digitalcommons.uconn.edu/gs_theses/237.

The development of peptide–boron difluoride formazanate conjugates as fluorescence imaging agents. Neha Sharmaa, Stephanie M. Barbona, Tyler Lalondea, Ryan R. Maara, Mark Milneb, Joe B. Gilroy and Leonard G. Luyt. RSC Adv., 2020, 10, 18970-18977. May 19th, 2020. DOI: 10.1039/D0RA02104K

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