5 EO – VectorLabs https://staging.vectorlabs.com From linker design and synthesis, bioconjugated design and manufacturing, biomolecule labeling and functionalization, and detection system and solutions, our team is here to help you move forward, with impact. Wed, 22 Oct 2025 17:16:49 +0000 en-US hourly 1 https://wordpress.org/?v=6.9 https://staging.vectorlabs.com/wp-content/uploads/2024/11/V-favicon-02-100x100.png 5 EO – VectorLabs https://staging.vectorlabs.com 32 32 Bis-dPEG®₅, half benzyl half NHS ester https://staging.vectorlabs.com/products/bis-dpeg5-half-benzyl-half-nhs-ester/ Sat, 24 Feb 2024 02:07:30 +0000 https://staging.vectorlabs.com/?post_type=product&p=35542 Bis-dPEG®5, half benzyl half NHS ester, product number QBD-10237, is a short, discrete PEG (dPEG®) modification reagent designed for stepwise reactions with amines. Both ends of the single molecular weight PEG linker terminate with propionic acid groups. One end is functionalized as the N-hydroxysuccinimidyl (NHS) ester, while the other acid end is protected as the benzyl ester. The benzyl ester can be removed using hydrogen with a palladium catalyst.

The dPEG®5 linker increases conjugates' hydrodynamic volume and hydrophilicity. QBD-10237 is useful for crosslinking amines under controlled conditions, modifying peptides at N-termini or amine-functionalized side chains, and building supramolecular constructs that require flexible, hydrophilic spacers.

Specifications

Unit Size 100 mg, 1000 mg
Molecular Weight 525.55; single compound
Chemical formula C₂₅H₃₅NO₁₁
CAS 1263044-84-3
Purity > 97%
Spacers dPEG® Spacer is 19 atoms and 21.7 Å
Shipping Ambient
Typical solubility properties (for additional information contact Customer Support) Methylene chloride, DMAC, or DMSO.
Storage and handling -20°C; Always let come to room temperature before opening; be careful to limit exposure to moisture and restore under an inert atmosphere; stock solutions can be prepared with dry solvent and kept for several days (freeze when not in use). dPEG® pegylation compounds are generally hygroscopic and should be treated as such. This will be less noticeable with liquids, but the solids will become tacky and difficult to manipulate, if care is not taken to minimize air exposure.

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Description

Bis-dPEG®5, half benzyl half NHS ester, product number QBD-10237, is a short, discrete PEG (dPEG®) modification reagent designed for stepwise reactions with amines. Both ends of the single molecular weight PEG linker terminate with propionic acid groups. One end is functionalized as the N-hydroxysuccinimidyl (NHS) ester, while the other acid end is protected as the benzyl ester. The benzyl ester can be removed using hydrogen with a palladium catalyst.

The dPEG®5 linker increases conjugates’ hydrodynamic volume and hydrophilicity. QBD-10237 is useful for crosslinking amines under controlled conditions, modifying peptides at N-termini or amine-functionalized side chains, and building supramolecular constructs that require flexible, hydrophilic spacers.

Specifications

Unit Size100 mg, 1000 mg
Molecular Weight525.55; single compound
Chemical formulaC₂₅H₃₅NO₁₁
CAS1263044-84-3
Purity> 97%
SpacersdPEG® Spacer is 19 atoms and 21.7 Å
ShippingAmbient
Typical solubility properties (for additional information contact Customer Support)Methylene chloride, DMAC, or DMSO.
Storage and handling-20°C; Always let come to room temperature before opening; be careful to limit exposure to moisture and restore under an inert atmosphere; stock solutions can be prepared with dry solvent and kept for several days (freeze when not in use). dPEG® pegylation compounds are generally hygroscopic and should be treated as such. This will be less noticeable with liquids, but the solids will become tacky and difficult to manipulate, if care is not taken to minimize air exposure.

Documents

References

  1. Greg T. Hermanson, Bioconjugate Techniques, 3rd Edition, Elsevier, Waltham, MA 02451, 2013, ISBN 978-0-12-382239-0; See Chapter 18, Discrete PEG Reagents, pp. 787-821, for a full overview of the dPEG® products.

The post Bis-dPEG®₅, half benzyl half NHS ester appeared first on VectorLabs.

]]> Bis-dPEG®₅-acid https://staging.vectorlabs.com/products/bis-dpeg5-acid/ Sat, 24 Feb 2024 02:07:25 +0000 https://staging.vectorlabs.com/?post_type=product&p=35515 "Bis-dPEG®5-acid, product number QBD-10230, is a short, monodisperse, homobifunctional polyethylene glycol (PEG) compound designed for crosslinking amines. Two propionic acid groups terminate either end of a discrete PEG (dPEG®) chain. These two end groups can be coupled directly to amines using a carbodiimide such as EDC. Alternatively, the carboxylate ends can be functionalized as active esters. Reactive groups such as NHS, TFP, PFP, and HOBt are suitable for this purpose. Reaction with various amines follows the activation step.

The hydrophilic dPEG® spacer imparts water solubility and flexibility to molecules to which it is conjugated. Moreover, conjugates containing dPEG® moieties have increased hydrodynamic volume. Also, the non-immunogenic crosslinker reduces or eliminates the antigenicity of the resulting conjugates.

Peptides, proteins, amine-containing biomolecules, and small molecules with primary or secondary amine groups can all be crosslinked with Bis-dPEG®5-acid. It may also be useful for peptide stapling or as a building block for macromolecular constructs."

Specifications

Unit Size 100 mg, 1000 mg
Molecular Weight 338.35; single compound
Chemical formula C₁₄H₂₆O₉
CAS 439114-13-3
Purity > 98%
Spacers dPEG® Spacer is 19 atoms and 21.7 Å
Shipping Ambient
Typical solubility properties (for additional information contact Customer Support) Methylene chloride, DMAC, DMSO or water.
Storage and handling -20°C; Always let come to room temperature before opening; be careful to limit exposure to moisture and restore under an inert atmosphere; stock solutions can be prepared with dry solvent and kept for several days (freeze when not in use). dPEG® pegylation compounds are generally hygroscopic and should be treated as such. This will be less noticeable with liquids, but the solids will become tacky and difficult to manipulate, if care is not taken to minimize air exposure.

The post Bis-dPEG®₅-acid appeared first on VectorLabs.

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Description

Bis-dPEG®5-acid, product number QBD-10230, is a short, monodisperse, homobifunctional polyethylene glycol (PEG) compound designed for crosslinking amines. Two propionic acid groups terminate either end of a discrete PEG (dPEG®) chain. These two end groups can be coupled directly to amines using a carbodiimide such as EDC. Alternatively, the carboxylate ends can be functionalized as active esters. Reactive groups such as NHS, TFP, PFP, and HOBt are suitable for this purpose. Reaction with various amines follows the activation step.

The hydrophilic dPEG® spacer imparts water solubility and flexibility to molecules to which it is conjugated. Moreover, conjugates containing dPEG® moieties have increased hydrodynamic volume. Also, the non-immunogenic crosslinker reduces or eliminates the antigenicity of the resulting conjugates.

Peptides, proteins, amine-containing biomolecules, and small molecules with primary or secondary amine groups can all be crosslinked with Bis-dPEG®5-acid. It may also be useful for peptide stapling or as a building block for macromolecular constructs.

Specifications

Unit Size100 mg, 1000 mg
Molecular Weight338.35; single compound
Chemical formulaC₁₄H₂₆O₉
CAS439114-13-3
Purity> 98%
SpacersdPEG® Spacer is 19 atoms and 21.7 Å
ShippingAmbient
Typical solubility properties (for additional information contact Customer Support)Methylene chloride, DMAC, DMSO or water.
Storage and handling-20°C; Always let come to room temperature before opening; be careful to limit exposure to moisture and restore under an inert atmosphere; stock solutions can be prepared with dry solvent and kept for several days (freeze when not in use). dPEG® pegylation compounds are generally hygroscopic and should be treated as such. This will be less noticeable with liquids, but the solids will become tacky and difficult to manipulate, if care is not taken to minimize air exposure.

Documents

References

  1. Greg T. Hermanson, Bioconjugate Techniques, 3rd Edition, Elsevier, Waltham, MA 02451, 2013, ISBN 978-0-12-382239-0; See Chapter 18, Discrete PEG Reagents, pp. 787-821, for a full overview of the dPEG® products.

The post Bis-dPEG®₅-acid appeared first on VectorLabs.

]]> Bis-dPEG®₅-NHS ester https://staging.vectorlabs.com/products/bis-dpeg5-nhs-ester/ Sat, 24 Feb 2024 02:07:21 +0000 https://staging.vectorlabs.com/?post_type=product&p=35489 Bis-dPEG®5-NHS ester, product number QBD-10224, is a homobifunctional, amine-reactive, single molecular weight PEG crosslinker with a short, discrete PEG (dPEG®) chain length. Each end of the chain terminates as the N-hydroxysuccinimidyl (NHS) ester of propionic acid. Unlike traditional hydrophobic crosslinkers, Bis-dPEG®5-NHS ester will not cause problems such as aggregation, precipitation, and inactivation when conjugated to biomolecules. Various uses for Bis-dPEG®5-NHS ester have been published in the scientific literature.

NHS esters react with free amines such as the ε-amines of lysine. The optimal pH range for NHS esters to react with free amines is 7.0 – 7.5. However, NHS esters can react with free amines with pH as low as 6.5. NHS esters are susceptible to hydrolysis in aqueous media. As the pH increases, the hydrolysis rate of the ester increases. Thus, we strongly discourage storing Bis-dPEG®5-NHS ester in water or aqueous buffer. Instead, we recommend that customers make new solutions of the product as needed, use them immediately, and discard unused solutions after use. If customers desire to store the product in solution, we recommend the use of a pure, anhydrous, water-miscible solvent such as dimethyl sulfoxide (DMSO), N,N-dimethylacetamide (DMAC), or N,N-dimethylformamide (DMF). DMSO, DMAC, or DMF can be dried chemically or by storing for a minimum of 24 hours over 3 Å molecular sieves. With DMF, use only fresh solvent as the compound decomposes over time to form free amines that will react with the NHS esters.

Specifications

Unit Size 100 mg, 1000 mg
Molecular Weight 532.50; single compound
Chemical formula C₂₂H₃₂N₂O₁₃
CAS 756526-03-1
Purity > 97%
Spacers dPEG® Spacer is 19 atoms and 21.568 Å
Shipping Ambient
Typical solubility properties (for additional information contact Customer Support) Methylene chloride, Acetonitrile, DMAC or DMSO.
Storage and handling -20°C; Always let come to room temperature before opening; be careful to limit exposure to moisture and restore under an inert atmosphere; stock solutions can be prepared with dry solvent and kept for several days (freeze when not in use). dPEG® pegylation compounds are generally hygroscopic and should be treated as such. This will be less noticeable with liquids, but the solids will become tacky and difficult to manipulate, if care is not taken to minimize air exposure.

The post Bis-dPEG®₅-NHS ester appeared first on VectorLabs.

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Description

Bis-dPEG®5-NHS ester, product number QBD-10224, is a homobifunctional, amine-reactive, single molecular weight PEG crosslinker with a short, discrete PEG (dPEG®) chain length. Each end of the chain terminates as the N-hydroxysuccinimidyl (NHS) ester of propionic acid. Unlike traditional hydrophobic crosslinkers, Bis-dPEG®5-NHS ester will not cause problems such as aggregation, precipitation, and inactivation when conjugated to biomolecules. Various uses for Bis-dPEG®5-NHS ester have been published in the scientific literature.

NHS esters react with free amines such as the ε-amines of lysine. The optimal pH range for NHS esters to react with free amines is 7.0 – 7.5. However, NHS esters can react with free amines with pH as low as 6.5. NHS esters are susceptible to hydrolysis in aqueous media. As the pH increases, the hydrolysis rate of the ester increases. Thus, we strongly discourage storing Bis-dPEG®5-NHS ester in water or aqueous buffer. Instead, we recommend that customers make new solutions of the product as needed, use them immediately, and discard unused solutions after use. If customers desire to store the product in solution, we recommend the use of a pure, anhydrous, water-miscible solvent such as dimethyl sulfoxide (DMSO), N,N-dimethylacetamide (DMAC), or N,N-dimethylformamide (DMF). DMSO, DMAC, or DMF can be dried chemically or by storing for a minimum of 24 hours over 3 Å molecular sieves. With DMF, use only fresh solvent as the compound decomposes over time to form free amines that will react with the NHS esters.

Specifications

Unit Size100 mg, 1000 mg
Molecular Weight532.50; single compound
Chemical formulaC₂₂H₃₂N₂O₁₃
CAS756526-03-1
Purity> 97%
SpacersdPEG® Spacer is 19 atoms and 21.568 Å
ShippingAmbient
Typical solubility properties (for additional information contact Customer Support)Methylene chloride, Acetonitrile, DMAC or DMSO.
Storage and handling-20°C; Always let come to room temperature before opening; be careful to limit exposure to moisture and restore under an inert atmosphere; stock solutions can be prepared with dry solvent and kept for several days (freeze when not in use). dPEG® pegylation compounds are generally hygroscopic and should be treated as such. This will be less noticeable with liquids, but the solids will become tacky and difficult to manipulate, if care is not taken to minimize air exposure.

Documents

References

Greg T. Hermanson, Bioconjugate Techniques, 3rd Edition, Elsevier, Waltham, MA 02451, 2013, ISBN 978-0-12-382239-0; See Chapter 18, Discrete PEG Reagents, pp. 787-821, for a full overview of the dPEG® products.

Cyclic and dimeric gluten peptide analogues inhibiting DQ2-mediated antigen presentation in celiac disease. Jiang Xia, Elin Bergseng, Burkhard Fleckenstein, Matthew Siegel, Chu-Young Kim, Chaitan Khosla and Ludvig M. Sollid. Bioorganic & Medicinal Chemistry. 15 (2007) pp 6565–6573. July 25, 2007. DOI: 10.1016/j.bmc.2007.07.001.

Design of a Potent D-Peptide HIV-1 Entry Inhibitor with a Strong Barrier to Resistance. Brett D. Welch, J. Nicholas Francis, Joseph S. Redman, Suparna Paul, Matthew T. Weinstock, Jacqueline D. Reeves, Yolanda S. Lie, Frank G. Whitby, Debra M. Eckert, Christopher P. Hill, Michael J. Root, and Michael S. Kay. Journal of Virology. 2010, 84 (21) pp 11235–11244. November 2010. DOI: 10.1128/JVI.01339-10.

Ligand-regulated oligomerization of b2-adrenoceptors in a model lipid bilayer. Juan Jose´ Fung, Xavier Deupi, Leonardo Pardo, Xiao Jie Yao, Gisselle A Velez-Ruiz, Brian T DeVree, Roger K Sunahara and Brian K Kobilka. The EMBO Journal, 2009, 28, pp 3315–3328. September 17, 2009. DOI: 10.1038/emboj.2009.267.

Monitoring the switching of single BSA-ATTO 488 molecules covalently end-attached to a pH responsive PAA brush. Namik Akkilic, Robert Molenaar, Mireille M.A.E. Claessens, Christian Blum, and Wiebe M. de Vos. Langmuir. 2016, pp 1-10. August 15, 2016. DOI: 10.1021/acs.langmuir.6b01064.

G-Quadruplex Induction by the Hairpin Pyrrole–Imidazole Polyamide Dimer. Shunsuke Obata, Sefan Asamitsu, Kaori Hashiya, Toshikazu Bando, and Hiroshi Sugiyama. Biochemistry. 2018, 57, pp 498-502. December 13, 2017. DOI: 10.1021/acs.biochem.7b01059.

Surface Modification on Acoustic Wave Biosensors for Enhanced Specificity. Onursal Onen, Asad A. Ahmad, Rasim Guldiken and Nathan D. Gallant. Sensors. 2012, 12 pp 12317-12328. September 10, 2012. DOI: 10.3390/s120912317.

Surface Functionalization and Analysis Thereof for an Ovarian Cancer Diagnostic Biosensor. Asad Ali Ahmad. USF Scholor Commons. 2011, 1 (1) pp 297 416. June 21, 2011. DOI: scholarcommons.usf.edu/etd/2977.

Serum albumin ‘camouflage’ of plant virus based nanoparticles prevents their antibody recognition and enhances pharmacokinetics. Andrzej S. Pitek, Slater A. Jameson, Frank A. Veliz, Sourabh Shukla, and Nicole F. Steinmetz. Biomaterials. 2016, 89 pp 89-97, February 23, 2016. DOI: 10.1016/j.biomaterials.2016.02.032.

Interdomain regulation of the ATPase activity of the ABC transporter hemolysin B from E. coli. Sven Reimann, Gereon Poschmann, Kerstin Kanonenbera, Kai Stuhler, Sander H.J. Smits, and Lutz Schmitt. Biochemical Journal. 2016, June 8, 2016. DOI: 10.1042/BCJ20160154.

Redox-Responsive Self-Assembly of Amphiphilic Multiblock Rod–Coil Polymers. Taek-Gyoung Kim, Chingu Kim, and Ji-Woong Park. Macromolecules. 2017, 50 (20) pp 8185–8191. 10/4/2017. DOI: 10.1021/acs.macromol.7b01650.

Toward the Development of GE11-Based Radioligands for Imaging of Epidermal Growth Factor Receptor-Positive Tumors. Benedikt Judmann, Diana Braun, Ralf Schirrmacher, Björn Wängler, Gert Fricker, and Carmen Wängler. ACS Omega 2022, 7, 31, 27690–27702. 2022. https://doi.org/10.1021/acsomega.2c03407

Multivalent Benzamidine Molecules for Plasmin Inhibition: Effect of Valency and Linker Length. Tanmaye Nallan Chakravarthula, Dr. Ziqian Zeng, Prof. Nathan J. Alves. ChemMedChem, Volume 17, Issue 22, (2022), 09/16/2022, https://doi.org/10.1002/cmdc.202200364.

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]]> Acid-dPEG®₅-NHS ester https://staging.vectorlabs.com/products/acid-dpeg5-nhs-ester/ Sat, 24 Feb 2024 02:04:52 +0000 https://staging.vectorlabs.com/?post_type=product&p=35062 Acid-dPEG®5-NHS ester, product number QBD-10109, is an amine-reactive crosslinker that is designed to facilitate intermolecular crosslinking with free amines at each end of the crosslinker through a single molecular weight, discrete polyethylene glycol (dPEG®) backbone. Because one end of the chain terminates in a propionic acid group while the other end terminates as the N-hydroxysuccinimidyl (NHS) ester of propionic acid, this product can effectively overcome the problems typically afflicting homobifunctional crosslinkers. Moreover, the carboxylic acid end can remain unmodified, allowing the carboxylic acid to be used as a charge modifier in supramolecular and bioconjugate construction. Furthermore, the amphiphilic dPEG® backbone imparts water solubility to conjugate molecules.

Specifications

Unit Size 100 mg, 1000 mg
Molecular Weight 435.42; single compound
Chemical formula C₁₈H₂₉NO₁₁
CAS N/A
Purity > 97%
Spacers dPEG® Spacer is 19 atoms and 21.6 Å
Shipping Ambient
Typical solubility properties (for additional information contact Customer Support) Methylene chloride, DMAC or DMSO.
Storage and handling -20°C; Always let come to room temperature before opening; be careful to limit exposure to moisture and restore under an inert atmosphere; stock solutions can be prepared with dry solvent and kept for several days (freeze when not in use). dPEG® pegylation compounds are generally hygroscopic and should be treated as such. This will be less noticeable with liquids, but the solids will become tacky and difficult to manipulate, if care is not taken to minimize air exposure.

The post Acid-dPEG®₅-NHS ester appeared first on VectorLabs.

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Description

Acid-dPEG®5-NHS ester, product number QBD-10109, is an amine-reactive crosslinker that is designed to facilitate intermolecular crosslinking with free amines at each end of the crosslinker through a single molecular weight, discrete polyethylene glycol (dPEG®) backbone. Because one end of the chain terminates in a propionic acid group while the other end terminates as the N-hydroxysuccinimidyl (NHS) ester of propionic acid, this product can effectively overcome the problems typically afflicting homobifunctional crosslinkers. Moreover, the carboxylic acid end can remain unmodified, allowing the carboxylic acid to be used as a charge modifier in supramolecular and bioconjugate construction. Furthermore, the amphiphilic dPEG® backbone imparts water solubility to conjugate molecules.

Specifications

Unit Size 100 mg, 1000 mg
Molecular Weight 435.42; single compound
Chemical formula C₁₈H₂₉NO₁₁
CAS N/A
Purity > 97%
Spacers dPEG® Spacer is 19 atoms and 21.6 Å
Shipping Ambient
Typical solubility properties (for additional information contact Customer Support) Methylene chloride, DMAC or DMSO.
Storage and handling -20°C; Always let come to room temperature before opening; be careful to limit exposure to moisture and restore under an inert atmosphere; stock solutions can be prepared with dry solvent and kept for several days (freeze when not in use). dPEG® pegylation compounds are generally hygroscopic and should be treated as such. This will be less noticeable with liquids, but the solids will become tacky and difficult to manipulate, if care is not taken to minimize air exposure.

Documents

References

  1. Greg T. Hermanson, Bioconjugate Techniques, 3rd Edition, Elsevier, Waltham, MA 02451, 2013, ISBN 978-0-12-382239-0; See Chapter 18, Discrete PEG Reagents, pp. 787-821, for a full overview of the dPEG® products.

The post Acid-dPEG®₅-NHS ester appeared first on VectorLabs.

]]> Fmoc-N-amido-dPEG®₅-acid https://staging.vectorlabs.com/products/fmoc-n-amido-dpeg5-acid/ Sat, 24 Feb 2024 02:04:37 +0000 https://staging.vectorlabs.com/?post_type=product&p=34879 "Fmoc-N-amido-dPEG®5-acid, product number QBD-10053, is one of a broad line of products designed for use in peptide synthesis. The short (19 atoms), discrete PEG (dPEG®) spacer is functionalized with a propionic acid group on one end and Fmoc-protected amine on the other. The product can be added to the N-terminus of a growing peptide chain or to a primary-amine-functionalized side chain of an amino acid such as lysine. The dPEG®5 spacer imparts water solubility to the peptide to which it is conjugated.

QBD-10053 permits our customers to insert a dPEG® spacer into a peptide chain using familiar Fmoc chemistry using solid phase or solution phase chemistry. The dPEG® compound can be inserted at either end of the peptide chain or in the middle of two amino acid sequences to provide a flexible linker between distinct functional peptides. Additionally, the dPEG® spacer can be used to provide spacing in a synthetic construct where steric hindrance is a problem. The amphiphilic nature of dPEG® products means that the construct gains hydrodynamic volume and water solubility while remaining soluble in organic solvent. The Fmoc protecting group removes easily with a solution of piperidine in N,N-dimethylformamide (DMF)."

Specifications

Unit Size 1000 mg
Molecular Weight 531.59; single compound
Chemical formula C₂₈H₃₇NO₉
CAS
882847-32-7
Purity > 98%
Spacers
dPEG® Spacer is 19 atoms and 21.6 Å
Shipping
Ambient
Typical solubility properties (for additional information contact Customer Support) Methylene chloride, Acetontrile, DMAC or DMSO.
Storage and handling -20°C; Always let come to room temperature before opening; be careful to limit exposure to moisture and restore under an inert atmosphere; stock solutions can be prepared with dry solvent and kept for several days (freeze when not in use). dPEG® pegylation compounds are generally hygroscopic and should be treated as such. This will be less noticeable with liquids, but the solids will become tacky and difficult to manipulate, if care is not taken to minimize air exposure.

The post Fmoc-N-amido-dPEG®₅-acid appeared first on VectorLabs.

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Description

Fmoc-N-amido-dPEG®5-acid, product number QBD-10053, is one of a broad line of products designed for use in peptide synthesis. The short (19 atoms), discrete PEG (dPEG®) spacer is functionalized with a propionic acid group on one end and Fmoc-protected amine on the other. The product can be added to the N-terminus of a growing peptide chain or to a primary-amine-functionalized side chain of an amino acid such as lysine. The dPEG®5 spacer imparts water solubility to the peptide to which it is conjugated.

QBD-10053 permits our customers to insert a dPEG® spacer into a peptide chain using familiar Fmoc chemistry using solid phase or solution phase chemistry. The dPEG® compound can be inserted at either end of the peptide chain or in the middle of two amino acid sequences to provide a flexible linker between distinct functional peptides. Additionally, the dPEG® spacer can be used to provide spacing in a synthetic construct where steric hindrance is a problem. The amphiphilic nature of dPEG® products means that the construct gains hydrodynamic volume and water solubility while remaining soluble in organic solvent. The Fmoc protecting group removes easily with a solution of piperidine in N,N-dimethylformamide (DMF).

Specifications

Unit Size 1000 mg
Molecular Weight 531.59; single compound
Chemical formula C₂₈H₃₇NO₉
CAS
882847-32-7
Purity > 98%
Spacers
dPEG® Spacer is 19 atoms and 21.6 Å
Shipping
Ambient
Typical solubility properties (for additional information contact Customer Support) Methylene chloride, Acetontrile, DMAC or DMSO.
Storage and handling -20°C; Always let come to room temperature before opening; be careful to limit exposure to moisture and restore under an inert atmosphere; stock solutions can be prepared with dry solvent and kept for several days (freeze when not in use). dPEG® pegylation compounds are generally hygroscopic and should be treated as such. This will be less noticeable with liquids, but the solids will become tacky and difficult to manipulate, if care is not taken to minimize air exposure.

Documents

References

  1. Greg T. Hermanson, Bioconjugate Techniques, 3rd Edition, Elsevier, Waltham, MA 02451, 2013, ISBN 978-0-12-382239-0; See Chapter 18, Discrete PEG Reagents, pp. 787-821, for a full overview of the dPEG® products.

  2. Design of a modular tetrameric scaffold for the synthesis of membrane-localized D-peptide inhibitors of HIV-1 entry. J. Nicholas Francis, Joseph S Redman, Debra M Eckert, and Michael S. Kay. Bioconjugate Chemistry. 2012, 23 (6), pp 1252-1258. May 1, 2012. DOI: 10.1021/bc300076f.

  3. Enhanced Cellular Uptake of Peptide-Targeted Nanoparticles through Increased Peptide Hydrophilicity and Optimized Ethylene Glycol Peptide-Linker Length. Jared F. Stefanick, Jonathan D. Ashley, and Basar Bilgicer. ACS Nano. 2013, 7 (9) pp 8115–8127. August 29, 2013. DOI: 10.1021/nn4033954.

The post Fmoc-N-amido-dPEG®₅-acid appeared first on VectorLabs.

]]> Tetrazine-PEG5-NHS Ester https://staging.vectorlabs.com/products/tetrazine-peg5-nhs-ester/ Tue, 19 Sep 2023 17:31:43 +0000 https://staging.vectorlabs.com/?post_type=product&p=21002 Description

Tetrazine-PEG5-NHS Ester is an amine-reactive labeling reagent often used for modification of proteins, peptides, or amine-modified oligonucleotides with tetrazine moiety. Hydrogen substituted tetrazines demonstrate exceptionally fast kinetics, generally at least 10 fold faster compared to methyl substituted tetrazines. The aqueous solubility of this reagent is substantially enhanced by a hydrophilic polyethylene glycol (PEG5) spacer arm.

Specifications

Unit Size 4 x 2 mg, 10 mg, 25 mg, 100 mg
Molecular weight 604.61
Chemical composition C27H36N9O10
CAS 1682653-80-0
Solubility DMSO, DMF, DCM, THF, Chloroform
Purity >95% (HPLC)
Appearance Red amorphous solid to red oil
Storage Conditions -20°C. Desiccate
Shipping Conditions Ambient temperature

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Description

Tetrazine-PEG5-NHS Ester is an amine-reactive labeling reagent often used for modification of proteins, peptides, or amine-modified oligonucleotides with tetrazine moiety. Hydrogen substituted tetrazines demonstrate exceptionally fast kinetics, generally at least 10 fold faster compared to methyl substituted tetrazines. The aqueous solubility of this reagent is substantially enhanced by a hydrophilic polyethylene glycol (PEG5) spacer arm.

Specifications

Unit Size4 x 2 mg, 10 mg, 25 mg, 100 mg
Molecular weight604.61
Chemical compositionC27H36N9O10
CAS1682653-80-0
SolubilityDMSO, DMF, DCM, THF, Chloroform
Purity>95% (HPLC)
AppearanceRed amorphous solid to red oil
Storage Conditions-20°C. Desiccate
Shipping ConditionsAmbient temperature

Documents

Selected References

  1. Ingham, E. S., et al. (2020). Positron emission tomography imaging of novel AAV capsids maps rapid brain accumulation. Nat Commun, 11, 2102. [Nature Communications]

The post Tetrazine-PEG5-NHS Ester appeared first on VectorLabs.

]]> DBCO-PEG5-Acid https://staging.vectorlabs.com/products/dbco-peg5-acid/ Tue, 19 Sep 2023 17:18:08 +0000 https://staging.vectorlabs.com/?post_type=product&p=20721 Description

DBCO-PEG5-Acid is an amine-reactive, non-activated building block with enhanced solubility in aqueous media used for derivatizing amine-containing molecule in the presence of activators (e.g. EDC, or HATU) through a stable amide bond.

DBCO reagents are the most commonly used substrates for strain-promoted click reactions. DBCO compounds react with azide functionalized compounds or biomolecules without the need for a Cu(I) catalyst to result in a stable triazole linkage.

Specifications

Unit Size 25 mg, 100 mg, 500 mg
Molecular weight 596.67
Chemical composition C32N40N2O9
CAS N/A
Solubility DMSO, DMF, DCM, THF, Chloroform
Purity >95% (HPLC)
Appearance Yellow to slightly orange oil
Storage Conditions -20°C. Desiccate
Shipping Conditions Frozen

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Description

DBCO-PEG5-Acid is an amine-reactive, non-activated building block with enhanced solubility in aqueous media used for derivatizing amine-containing molecule in the presence of activators (e.g. EDC, or HATU) through a stable amide bond.

DBCO reagents are the most commonly used substrates for strain-promoted click reactions. DBCO compounds react with azide functionalized compounds or biomolecules without the need for a Cu(I) catalyst to result in a stable triazole linkage.

Specifications

Unit Size25 mg, 100 mg, 500 mg
Molecular weight596.67
Chemical compositionC32N40N2O9
CASN/A
SolubilityDMSO, DMF, DCM, THF, Chloroform
Purity>95% (HPLC)
AppearanceYellow to slightly orange oil
Storage Conditions-20°C. Desiccate
Shipping ConditionsFrozen

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]]> DBCO-PEG5-NHS Ester https://staging.vectorlabs.com/products/dbco-peg5-nhs-ester/ Tue, 19 Sep 2023 17:17:16 +0000 https://staging.vectorlabs.com/?post_type=product&p=20703 Description

DBCO-PEG5-NHS Ester reacts specifically and efficiently with a primary amine (e.g., side chain of lysine residues or aminosilane-coated surfaces) at pH 7-9 to form a covalent bond. The hydrophilic polyethylene glycol (PEG) spacer arm imparts water solubility that is transferred to the labeled molecule, thus reducing aggregation of labeled proteins stored in solution. The PEG spacer arm provides a long and flexible connection that minimizes steric hindrance involved with ligation to complementary azide-containing molecules.

Specifications

Unit Size 4x2mg, 20mg, 25mg, 100mg, 500mg, 1000mg
Molecular weight 693.74
Chemical composition C36H43N3O11
CAS 1378531-80-6
Solubility DMSO, DMF, DCM, THF, Chloroform
Purity >95% (HPLC)
Appearance Viscous oil or amorphous solid
Storage Conditions -20°C. Desiccate
Shipping Conditions Ambient temperature

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Description

DBCO-PEG5-NHS Ester reacts specifically and efficiently with a primary amine (e.g., side chain of lysine residues or aminosilane-coated surfaces) at pH 7-9 to form a covalent bond. The hydrophilic polyethylene glycol (PEG) spacer arm imparts water solubility that is transferred to the labeled molecule, thus reducing aggregation of labeled proteins stored in solution. The PEG spacer arm provides a long and flexible connection that minimizes steric hindrance involved with ligation to complementary azide-containing molecules.

Specifications

Unit Size4x2mg, 20mg, 25mg, 100mg, 500mg, 1g
Molecular weight693.74
Chemical compositionC36H43N3O11
CAS1378531-80-6
SolubilityDMSO, DMF, DCM, THF, Chloroform
Purity>95% (HPLC)
AppearanceViscous oil or amorphous solid
Storage Conditions-20°C. Desiccate
Shipping ConditionsFrozen

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Selected References

  1. Tracy, C. M., et al. (2019). Cysteine-Directed Bioconjugation of a Platinum(II)-Acridine Anticancer Agent. Inorg Chem., 58 (1), 43-46. [PubMed]
  2. Allo, B., et al. (2018). Clickable and High-Sensitivity Metal-Containing Tags for Mass Cytometry. Bioconjugate Chem. , 29(6), 2028-38. [PubMed]
  3. Nedrow-Byerst, J.R., et al. (2013). PSMA-targeted SPECT agents: mode of binding effect on in vitro performance. Prostate., 73(4), 355-62-9. [PubMed]

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]]> DBCO-PEG5-TFP Ester https://staging.vectorlabs.com/products/dbco-peg5-tfp-ester/ Tue, 19 Sep 2023 17:16:51 +0000 https://staging.vectorlabs.com/?post_type=product&p=20695 Description

DBCO-PEG5-TFP Ester is an amine-reactive, labeling reagent used to modify proteins, antibodies, and other amine-containing biopolymers. The hydrophilic polyethylene glycol (PEG) spacer arm provides a long and flexible connection that minimizes steric hindrance involved with ligation to complementary azide-containing molecules.

The N-hydroxysuccinimide (NHS, also known as HOSu) esters of carboxylic acids are the most widely used reactive esters for modifying amine-containing peptides and proteins. NHS esters are notorious for having a short, pH-dependent half-life in aqueous media. A 2,3,5,6-tetrafluorophenol (TFP) is a different type of reactive ester that displays much better stability toward hydrolysis in aqueous media resulting in more efficiency and better reproducible labeling of biopolymers. TFP ester of carboxylic acids react with primary amines at the same rate as NHS ester forming covalent amide bond that is identical to one formed by the reaction between primary amines and NHS esters or sulfo-NHS esters.

DBCO reagents are the most commonly used substrates for strain-promoted azide-alkyne click chemistry. DBCO compounds react with azide functionalized compounds or biomolecules without the need for a Cu(I) catalyst to result in a stable triazole linkage.

Specifications

Unit Size 4 x 2 mg, 10 mg, 25 mg, 100 mg, 500 mg
Molecular weight 744.27
Chemical composition C38H40F4N2O9
CAS N/A
Solubility DMF, DMSO, DCM, THF, Chloroform
Purity >95% (HPLC)
Appearance Viscous oil
Storage Conditions -20°C. Desiccate
Shipping Conditions Frozen

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DBCO-PEG5-TFP Ester is an amine-reactive, labeling reagent used to modify proteins, antibodies, and other amine-containing biopolymers. The hydrophilic polyethylene glycol (PEG) spacer arm provides a long and flexible connection that minimizes steric hindrance involved with ligation to complementary azide-containing molecules.

Description

DBCO-PEG5-TFP Ester is an amine-reactive, labeling reagent used to modify proteins, antibodies, and other amine-containing biopolymers. The hydrophilic polyethylene glycol (PEG) spacer arm provides a long and flexible connection that minimizes steric hindrance involved with ligation to complementary azide-containing molecules.

The N-hydroxysuccinimide (NHS, also known as HOSu) esters of carboxylic acids are the most widely used reactive esters for modifying amine-containing peptides and proteins. NHS esters are notorious for having a short, pH-dependent half-life in aqueous media. A 2,3,5,6-tetrafluorophenol (TFP) is a different type of reactive ester that displays much better stability toward hydrolysis in aqueous media resulting in more efficiency and better reproducible labeling of biopolymers. TFP ester of carboxylic acids react with primary amines at the same rate as NHS ester forming covalent amide bond that is identical to one formed by the reaction between primary amines and NHS esters or sulfo-NHS esters.

DBCO reagents are the most commonly used substrates for strain-promoted azide-alkyne click chemistry. DBCO compounds react with azide functionalized compounds or biomolecules without the need for a Cu(I) catalyst to result in a stable triazole linkage.

Specifications

Unit Size4 x 2 mg, 10 mg, 25 mg, 100 mg, 500 mg
Molecular weight744.27
Chemical compositionC38H40F4N2O9
CASN/A
SolubilityDMF, DMSO, DCM, THF, Chloroform
Purity>95% (HPLC)
AppearanceViscous oil
Storage Conditions-20°C. Desiccate
Shipping ConditionsFrozen

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