Water-soluble, substrate for sortase mediated labeling of proteins. Sortase catalyzes a transpeptidase reaction between a specific internal sequence of a protein and an amine group present on the N-terminus of triglycine recently has become an area of great interest. This method of labeling proteins has been denoted as “Sortagging”.

Proteins conjugated to DBCO-Gly-Gly-Gly can be further modified with azide-containing molecules creating site-specific protein conjugates. Examples of creating protein conjugates using sortagging include site-specifically PEGylating proteins,¹ site-specific protein-lipid conjugates,² and constructing peptides and glycosylphosphatidylinositol chimeras.³ Sortase has also been used in peptide synthesis to cyclize peptides to create macrocyclic peptides, glycopeptides⁴ and protein−protein conjugates.

Specifications

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Sulfo DBCO-PEG4-TFP Ester is a water-soluble, amine-reactive reagent that enables simple and efficient incorporation of DBCO moiety onto proteins, peptides or other amine-containing molecules. The hydrophilic sulfonated spacer arm greatly improves water solubility of DBCO derivatized molecules, in many cases making it completely soluble in aqueous media. The hydrophilic PEG spacer arm improves labeling efficiency and provides a long and flexible connection.

Specifications

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Sulfo DBCO-PEG4-Maleimide is a water-soluble, sulfhydryl-reactive reagent that enables simple and efficient incorporation of DBCO moiety onto cysteine-containing peptides or other thiol-containing molecules. The hydrophilic sulfonated spacer arm greatly improves water solubility of DBCO derivatized molecules, in many cases making it completely soluble in aqueous media. The hydrophilic PEG spacer arm improves labeling efficiency and provides a long and flexible connection.

Specifications

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Sulfo DBCO-PEG4-Amine is a water-soluble reagent used to derivatize carboxyl-containing molecules or activated esters (e.g. NHS esters) with DBCO moiety through a stable amide bond. The hydrophilic sulfonated spacer arm greatly improves water solubility of DBCO derivatized molecules, in many cases making it completely soluble in aqueous media. The PEG spacer arm provides a long and flexible connection.

Specifications

The post Sulfo DBCO-PEG4-Amine appeared first on VectorLabs.