2 EO – VectorLabs https://staging.vectorlabs.com From linker design and synthesis, bioconjugated design and manufacturing, biomolecule labeling and functionalization, and detection system and solutions, our team is here to help you move forward, with impact. Wed, 22 Oct 2025 17:18:52 +0000 en-US hourly 1 https://wordpress.org/?v=6.9 https://staging.vectorlabs.com/wp-content/uploads/2024/11/V-favicon-02-100x100.png 2 EO – VectorLabs https://staging.vectorlabs.com 32 32 Bis-dPEG®₂-NHS ester https://staging.vectorlabs.com/products/bis-dpeg2-nhs-ester/ Sat, 24 Feb 2024 02:12:05 +0000 https://staging.vectorlabs.com/?post_type=product&p=36712 Bis-dPEG®2-NHS ester, product number QBD-10724, is a homobifunctional, amine-reactive, crosslinking reagent. Each end of the short (10 atoms, 11.0 Å), single molecular weight, discrete PEG (dPEG®) linker is functionalized as the N-hydroxysuccinimidyl (NHS) ester of a propionic acid group. The optimal pH range for reacting the end groups is 7.0 - 7.5. The product is soluble in water and many organic solvents; however, in water, it hydrolyzes rapidly. Therefore, we advise against storing aqueous stock solutions of this product. Rather, unused material in aqueous media should be discarded promptly.

Specifications

Unit Size 100mg, 1000mg
Molecular Weight 400.34; single compound
Chemical formula C₁₆H₂₀N₂O₁₀
CAS 65869-63-8
Purity > 98%
Spacers dPEG® Spacer is 10 atoms and 11.0 Å
Shipping Ambient
Typical solubility properties (for additional information contact Customer Support) Methylene chloride, Acetonitrile, DMAC or DMSO.
Storage and handling -20°C; Always let come to room temperature before opening; be careful to limit exposure to moisture and restore under an inert atmosphere; stock solutions can be prepared with dry solvent and kept for several days (freeze when not in use). dPEG® pegylation compounds are generally hygroscopic and should be treated as such. This will be less noticeable with liquids, but the solids will become tacky and difficult to manipulate, if care is not taken to minimize air exposure.

The post Bis-dPEG®₂-NHS ester appeared first on VectorLabs.

]]>

Description

Bis-dPEG®2-NHS ester, product number QBD-10724, is a homobifunctional, amine-reactive, crosslinking reagent. Each end of the short (10 atoms, 11.0 Å), single molecular weight, discrete PEG (dPEG®) linker is functionalized as the N-hydroxysuccinimidyl (NHS) ester of a propionic acid group. The optimal pH range for reacting the end groups is 7.0 – 7.5. The product is soluble in water and many organic solvents; however, in water, it hydrolyzes rapidly. Therefore, we advise against storing aqueous stock solutions of this product. Rather, unused material in aqueous media should be discarded promptly.

Specifications

Unit Size100mg, 1000mg
Molecular Weight400.34; single compound
Chemical formulaC₁₆H₂₀N₂O₁₀
CAS65869-63-8
Purity> 98%
SpacersdPEG® Spacer is 10 atoms and 11.0 Å
ShippingAmbient
Typical solubility properties (for additional information contact Customer Support)Methylene chloride, Acetonitrile, DMAC or DMSO.
Storage and handling-20°C; Always let come to room temperature before opening; be careful to limit exposure to moisture and restore under an inert atmosphere; stock solutions can be prepared with dry solvent and kept for several days (freeze when not in use). dPEG® pegylation compounds are generally hygroscopic and should be treated as such. This will be less noticeable with liquids, but the solids will become tacky and difficult to manipulate, if care is not taken to minimize air exposure.



Documents

References

Greg T. Hermanson, Bioconjugate Techniques, 3rd Edition, Elsevier, Waltham, MA 02451, 2013, ISBN 978-0-12-382239-0; See Chapter 18, Discrete PEG Reagents, pp. 787-821, for a full overview of the dPEG® products.

Multivalent Benzamidine Molecules for Plasmin Inhibition: Effect of Valency and Linker Length. Tanmaye Nallan Chakravarthula, Dr. Ziqian Zeng, Prof. Nathan J. Alves. ChemMedChem, Volume 17, Issue 22, (2022), 09/16/2022, https://doi.org/10.1002/cmdc.202200364.

The post Bis-dPEG®₂-NHS ester appeared first on VectorLabs.

]]> MAL-dPEG®₂-TFP ester https://staging.vectorlabs.com/products/mal-dpeg2-tfp-ester/ Sat, 24 Feb 2024 02:11:34 +0000 https://staging.vectorlabs.com/?post_type=product&p=36439 MAL-dPEG®2-TFP ester, product number QBD-10549, is a crosslinking reagent that joins free amines to free thiols (sulfhydryl groups) across a short (16 atoms), single molecular weight, discrete PEG (dPEG®) linker. The sulfhydryl groups react with a maleimido group via a Michael addition reaction. The amines form amide bonds with the crosslinker by nucleophilic substitution of the 2,3,5,6-tetrafluorophenyl (TFP) ester of the terminal propionic acid group.
The conjugation of free amines to free thiols is one of the most popular, most useful crosslinking reactions in bioconjugate chemistry is the conjugation of free amines to free thiols. These reactions require heterobifunctional reagents that bridge the two groups. Typical crosslinkers are hydrophobic. Vector Laboratories' dPEG® crosslinking products are water-soluble, amphiphilic, single molecular weight PEG compounds with discrete chain lengths. These hydrophilic, non-immunogenic, dPEG® crosslinkers prevent the problems with aggregation that arise when using traditional, hydrophobic crosslinkers.

Published studies have shown that TFP esters are more hydrolytically stable in aqueous buffers than N-hydroxysuccinimidyl (NHS) esters and are more reactive toward free amines. TFP esters react under the same conditions as NHS esters. However, the optimal pH range for TFP esters (7.5 – 8.0) is slightly higher than for NHS esters (7.0 – 7.5). Amide bond formation between the TFP-activated propionic acid group of MAL-dPEG®2-TFP ester and a free amine will be slightly slower at a suboptimal pH compared to the reaction rate within the optimum pH range.

The maleimide end of MAL-dPEG®2-TFP ester, product number QBD-10549, reacts optimally with a sulfhydryl at pH 6.5 – 7.5. Conduct the conjugation at the lowest reasonable pH within this range. Above pH 7.5, free amines compete with free thiols at the maleimide reaction site, which may confound the data. Moreover, at higher pH values, the maleimide ring may open to form unreactive maleamic acid.

MAL-dPEG®2-TFP ester, product number QBD-10549, can be used the same way and in the same applications as the equivalent NHS esters. Possible uses for this product include the following:
Coating nanoparticle surfaces;
Tethering antibodies to atomic force microscopy (AFM) probes;
Constructing antibody-drug conjugates (ADCs);
Adding flexibility and water solubility to a peptide;
Increasing the water solubility and hydrodynamic volume of hydrophobic biomolecules;
Building supramolecular constructs; and,
Conjugating the TFP ester end of the molecule a liposomal surface and then using the free maleimide end of the molecule to attach a small molecule drug, peptide, or antibody for targeted delivery of a diagnostic or therapeutic package.

Specifications

Unit Size 100mg, 1000mg
Molecular Weight 476.38; single compound
Chemical formula C₂₀H₂₀F₄N₂O₇
CAS
N/A
Purity > 98%
Spacers
dPEG® Spacer is 16 atoms and 17.3 Å
Shipping
Ambient
Typical solubility properties (for additional information contact Customer Support) Methylene Chloride, DMSO, DMAC, DMF, Methanol or Acetonitrile.
Storage and handling -20°C; Always let come to room temperature before opening; be careful to limit exposure to moisture and restore under an inert atmosphere; stock solutions can be prepared with dry solvent and kept for several days (freeze when not in use). dPEG® pegylation compounds are generally hygroscopic and should be treated as such. This will be less noticeable with liquids, but the solids will become tacky and difficult to manipulate, if care is not taken to minimize air exposure.

The post MAL-dPEG®₂-TFP ester appeared first on VectorLabs.

]]>

Description

MAL-dPEG®2-TFP ester, product number QBD-10549, is a crosslinking reagent that joins free amines to free thiols (sulfhydryl groups) across a short (16 atoms), single molecular weight, discrete PEG (dPEG®) linker. The sulfhydryl groups react with a maleimido group via a Michael addition reaction. The amines form amide bonds with the crosslinker by nucleophilic substitution of the 2,3,5,6-tetrafluorophenyl (TFP) ester of the terminal propionic acid group.
The conjugation of free amines to free thiols is one of the most popular, most useful crosslinking reactions in bioconjugate chemistry is the conjugation of free amines to free thiols. These reactions require heterobifunctional reagents that bridge the two groups. Typical crosslinkers are hydrophobic. Vector Laboratories’ dPEG® crosslinking products are water-soluble, amphiphilic, single molecular weight PEG compounds with discrete chain lengths. These hydrophilic, non-immunogenic, dPEG® crosslinkers prevent the problems with aggregation that arise when using traditional, hydrophobic crosslinkers.

Published studies have shown that TFP esters are more hydrolytically stable in aqueous buffers than N-hydroxysuccinimidyl (NHS) esters and are more reactive toward free amines. TFP esters react under the same conditions as NHS esters. However, the optimal pH range for TFP esters (7.5 – 8.0) is slightly higher than for NHS esters (7.0 – 7.5). Amide bond formation between the TFP-activated propionic acid group of MAL-dPEG®2-TFP ester and a free amine will be slightly slower at a suboptimal pH compared to the reaction rate within the optimum pH range.

The maleimide end of MAL-dPEG®2-TFP ester, product number QBD-10549, reacts optimally with a sulfhydryl at pH 6.5 – 7.5. Conduct the conjugation at the lowest reasonable pH within this range. Above pH 7.5, free amines compete with free thiols at the maleimide reaction site, which may confound the data. Moreover, at higher pH values, the maleimide ring may open to form unreactive maleamic acid.

MAL-dPEG®2-TFP ester, product number QBD-10549, can be used the same way and in the same applications as the equivalent NHS esters. Possible uses for this product include the following:
Coating nanoparticle surfaces;
Tethering antibodies to atomic force microscopy (AFM) probes;
Constructing antibody-drug conjugates (ADCs);
Adding flexibility and water solubility to a peptide;
Increasing the water solubility and hydrodynamic volume of hydrophobic biomolecules;
Building supramolecular constructs; and,
Conjugating the TFP ester end of the molecule a liposomal surface and then using the free maleimide end of the molecule to attach a small molecule drug, peptide, or antibody for targeted delivery of a diagnostic or therapeutic package.

Specifications

Unit Size100mg, 1000mg
Molecular Weight476.38; single compound
Chemical formulaC₂₀H₂₀F₄N₂O₇
CAS
N/A
Purity> 98%
Spacers
dPEG® Spacer is 16 atoms and 17.3 Å
Shipping
Ambient
Typical solubility properties (for additional information contact Customer Support)Methylene Chloride, DMSO, DMAC, DMF, Methanol or Acetonitrile.
Storage and handling-20°C; Always let come to room temperature before opening; be careful to limit exposure to moisture and restore under an inert atmosphere; stock solutions can be prepared with dry solvent and kept for several days (freeze when not in use). dPEG® pegylation compounds are generally hygroscopic and should be treated as such. This will be less noticeable with liquids, but the solids will become tacky and difficult to manipulate, if care is not taken to minimize air exposure.



Documents

References

Greg T. Hermanson, Bioconjugate Techniques, 3rd Edition, Elsevier, Waltham, MA 02451, 2013, ISBN 978-0-12-382239-0; See Chapter 18, Discrete PEG Reagents, pp. 787-821, for a full overview of the dPEG® products.

The post MAL-dPEG®₂-TFP ester appeared first on VectorLabs.

]]> MAL-dPEG®₂-NHS ester https://staging.vectorlabs.com/products/mal-dpeg2-nhs-ester/ Sat, 24 Feb 2024 02:07:48 +0000 https://staging.vectorlabs.com/?post_type=product&p=35668 MAL-dPEG®2-NHS ester, product number QBD-10266, is a thiol-reactive and amine-reactive crosslinking reagent that joins a sulfhydryl to a free amine. The sulfhydryl groups react with a maleimide group via a Michael addition reaction. The amines form amide bonds with the crosslinker by nucleophilic substitution of the N-hydroxysuccinimidyl (NHS) ester of a carboxylic acid group. The maleimide and NHS functional groups on the crosslinking compound sit at either end of a short, discrete-length polyethylene glycol chain (dPEG®).

The reaction of the maleimide end of MAL-dPEG®2-NHS ester proceeds optimally at pH 7.0 – 7.5, though it can go as low as pH 6.5. Use the lowest reasonable pH within this range. Above pH 7.5, free amines compete with free thiols at the maleimide reaction site, causing confusing results. Moreover, at higher pH values, the maleimide ring may open to form unreactive maleamic acid.

The use of MAL-dPEG®2-NHS ester has been published in many different scientific papers and patents. The following list highlights some of the more notable uses of this product:
Cell targeting;
Crosslinking peptides to passivated nanoparticle surfaces;
Stem cell membrane engineering;
Biosensor development;
Cell capture using aptamers;
Controlled, targeted delivery of siRNA;
Development of extracellular antibody-drug conjugates; and,
Development of multiplex assays.

Specifications

Unit Size 100 mg, 1000 mg
Molecular Weight 425.39; single compound
Chemical formula C₁₈H₂₃N₃O₉
CAS 955094-26-5
Purity > 98%
Spacers dPEG® Spacer is 16 atoms and 17.7 Å
Shipping Ambient
Typical solubility properties (for additional information contact Customer Support) Methylene chloride, Acetonitrile, DMAC or DMSO.
Storage and handling -20°C; Always let come to room temperature before opening; be careful to limit exposure to moisture and restore under an inert atmosphere; stock solutions can be prepared with dry solvent and kept for several days (freeze when not in use). dPEG® pegylation compounds are generally hygroscopic and should be treated as such. This will be less noticeable with liquids, but the solids will become tacky and difficult to manipulate, if care is not taken to minimize air exposure.

The post MAL-dPEG®₂-NHS ester appeared first on VectorLabs.

]]>

Description

MAL-dPEG®2-NHS ester, product number QBD-10266, is a thiol-reactive and amine-reactive crosslinking reagent that joins a sulfhydryl to a free amine. The sulfhydryl groups react with a maleimide group via a Michael addition reaction. The amines form amide bonds with the crosslinker by nucleophilic substitution of the N-hydroxysuccinimidyl (NHS) ester of a carboxylic acid group. The maleimide and NHS functional groups on the crosslinking compound sit at either end of a short, discrete-length polyethylene glycol chain (dPEG®).

The reaction of the maleimide end of MAL-dPEG®2-NHS ester proceeds optimally at pH 7.0 – 7.5, though it can go as low as pH 6.5. Use the lowest reasonable pH within this range. Above pH 7.5, free amines compete with free thiols at the maleimide reaction site, causing confusing results. Moreover, at higher pH values, the maleimide ring may open to form unreactive maleamic acid.

The use of MAL-dPEG®2-NHS ester has been published in many different scientific papers and patents. The following list highlights some of the more notable uses of this product:
Cell targeting;
Crosslinking peptides to passivated nanoparticle surfaces;
Stem cell membrane engineering;
Biosensor development;
Cell capture using aptamers;
Controlled, targeted delivery of siRNA;
Development of extracellular antibody-drug conjugates; and,
Development of multiplex assays.

Specifications

Unit Size100 mg, 1000 mg
Molecular Weight425.39; single compound
Chemical formulaC₁₈H₂₃N₃O₉
CAS955094-26-5
Purity> 98%
SpacersdPEG® Spacer is 16 atoms and 17.7 Å
ShippingAmbient
Typical solubility properties (for additional information contact Customer Support)Methylene chloride, Acetonitrile, DMAC or DMSO.
Storage and handling-20°C; Always let come to room temperature before opening; be careful to limit exposure to moisture and restore under an inert atmosphere; stock solutions can be prepared with dry solvent and kept for several days (freeze when not in use). dPEG® pegylation compounds are generally hygroscopic and should be treated as such. This will be less noticeable with liquids, but the solids will become tacky and difficult to manipulate, if care is not taken to minimize air exposure.

Documents

References

Greg T. Hermanson, Bioconjugate Techniques, 2nd Edition, Elsevier Inc., Burlington, MA 01803, April, 2008 (ISBN-13: 978-0-12-370501-3; ISBN-10: 0-12-370501-0); See pp. 276-335 for general description and use of heterobifunctional crosslinkers, as well as his specific discussion with protocols of our MAL-dPEG®x-NHS esters on pp. 718-722.

Greg T. Hermanson, Bioconjugate Techniques, 3rd Edition, Elsevier, Waltham, MA 02451, 2013, ISBN 978-0-12-382239-0; See Chapter 18, Discrete PEG Reagents, pp. 787-821, for a full overview of the dPEG® products.

Cell selective targeting of a simian virus 40 virus-like particle conjugated to epidermal growth factor. Yuichi Kitai, Hajime Fukuda, Teruya Enomoto, Yuki Asakawa, Takahiro Suzuki, Satoshi Inouye, Hiroshi Handa. Journal of Biotechnology. 2011, 155 (2) pp 251-256. Sept 10, 2011. DOI: 10.1016/j.jbiotec.2011.06.030.

Conjugation of Peptides to the Passivation Shell of Gold Nanoparticles for Targeting of Cell-Surface Receptors. Lisa Maus, Oliver Dick, Hilmar Bading, Joachim P. Spatz, and Roberto Fiammengo. ACS Nano. 2010, 4 (11), pp 6617–6628. October 12, 2010. DOI: 10.1021/nn101867w.

Stem cell membrane engineering for cell rolling using peptide conjugation and tuning of celleselectin interaction kinetics. Hao Cheng, Marta Byrska-Bishop, Cathy T. Zhang, Christian J. Kastrup, Nathaniel S. Hwang, Albert K. Tai, Won Woo Lee, Xiaoyang Xu, Matthias Nahrendorf , Robert Langer, Daniel G. Anderson. Biomaterials. 2012, 33 (20), pp 5004-5012. July 2012. DOI: 10.1016/j.biomaterials.2012.03.065.

Sensitive detection of small molecule–protein interactions on a metal-insulator-metal label-free biosensing platform. Amir Syahir, Kotaro Kajikawa and Hisakazu Mihara. Europe PubMed Central. 2012, 7 (8) pp 1867-1874. May 25, 2012. DOI: 10.1002/asia.201200138.

Aptamer-Containing Surfaces for Selective Capture of CD4 Expressing Cells. Qing Zhou, Ying Liu, Dong-Sik Shin, Jaime Silangcruz, Nazgul Tuleuova, and Alexander Revzin. Langmuir. 2012, 28 (34) pp 12544−12549. August 1, 2012. DOI: 10.1021/la2050338.

NIR light controlled photorelease of siRNA and its targeted intracellular delivery based on upconversion Nanoparticles. Yanmei Yang, Fang Liu, Xiaogang Liu and Bengang Xing. Nanoscale. 2013, 5 (1) pp 231-238. Oct 30, 2012. DOI: 10.1039/C2NR32835F.

Attachment of hydrogel microstructures and proteins to glass via thiol-terminated silanes. Jeong Hyun Seo, Dong-Sik Shin, Priam Mukundan, Alexander Revzin. Colloids and Surfaces B: Biointerfaces. 2012, 98 pp 1–6. October 1, 2012. DOI: org/10.1016/j.colsurfb.2012.03.025.

A sandwich-type DNA array platform for detection of GM targets in multiplex assay. Sena Cansız, Can Ozen, Ceren Bayrac, A. Tahir Bayrac, Fatma Gul, Murat Kavruk, Remziye Yılmaz, Fusun Eyidogan, Huseyin, Avni Oktem. European Food Research and Technology. 2012, 235 (3) pp 429-437. July 6, 2012. DOI: 10.1007/s00217-012-1767-y.

Extracellular Antibody Drug Conjugates Exploiting the Proximity of Two Proteins. David J Marshall, Scott S Harried, John L Murphy, Chad A Hall, Mohammed S Shekhani, Christophe Pain, Conner A Lyons, Antonella Chillemi, Fabio Malavsi, Homer L Pearce, Jon S Thorson, and James R Prudent. MT Open. 2016. July 19, 2016. DOI: 10.1038/mt.2016.119.

A simple DNA handle attachment method for single molecule mechanical manipulation experiments. Duyoung Min, Mark A. Arbing, Robert E. Jefferson, and James U. Bowie. Protein Science, 2016, 25 pp 1535-1544. May 24, 2016. DOI: 10.1002/pro.2952.

A virus-like particle vaccine platform elicits heightened and hastened local lung mucosal antibody production after a single dose. Laura E. Richert, Amy E. Servidb, Ann L. Harmsen, Agnieszka Rynda-Apple, Soo Han, James A. Wiley, Trevor Douglas, Allen G. Harmsen. Vaccine. 2012, 30 (24) pp 3653-3665. May 21, 2012. DOI: 10.1016/j.vaccine.2012.03.035.

The use of glass substrates with bi-functional silanes for designing micropatterned cell-secreted cytokine immunoassays. Seo JH, Chen Li, Verkhoturov SV, Schweikert EA, Revzin A. Biomaterials. 2011, 32 (23) pp 5478-5488. August 2011. DOI: 10.1016/j.biomaterials.2011.04.026.

Comparison between polyethylene glycol and zwitterionic polymers as antifouling coatings on wearable devices for selective antigen capture from biological tissue. Kye J. Robinson, Jacob W. Coffey, David A. Muller, Paul R. Young, Mark A. F. Kendall, Kristofer J. Thurecht, Lisbeth Grondahl, and Simon R. Corrie. Biointerphases. 2015, 10 (4) pp 04A305. December 2015. DOI: 10.1116/1.4932055.

A neutralized non-charged polyethylenimine-based system for efficient delivery of siRNA into heart without toxicity. Fang Wang, Lu Gao, Liu-Yi Meng, Jing-Ming Xie, Jing-Wei Xiong, and Ying Luo. ACS Applied Materials and Interfaces. 2016, pp 1-43. November 17, 2016. DOI: 10.1021/acsami.6b13295.

Drug-free albumin-triggered sensitization of cancer cells to anticancer drugs. LianLi, Jiyuan Yang, Sirima Soodvilaia, Jiawei Wang, Praneet Opanasopit, Jindřich Kopeček. Journal of Controlled Release. 2018, 293 (2019) pp 84-93. November 19, 2018. https://doi.org/10.1016/j.jconrel.2018.11.015.

The surface stress of biomedical silicones is a stimulant of cellular response. Zhu Cheng, Carolyn R. Shurer, Samuel Schmidt, Vivek K. Gupta, Grace Chuang, Jin Su, Amanda R. Watkins, Abhishek Shetty, Jason A. Spector, Chung-Yuen Hui, Heidi L. Reesink and Matthew J. Paszek. Science Advances. 2020. Vol. 6, no. 15, eaay0076. 10 Apr 2020. DOI: 10.1126/sciadv.aay0076.

Preclinical Development of a Fusion Peptide Conjugate as an HIV Vaccine Immunogen
Li Ou, Wing-Pui Kong, Gwo-Yu Chuang, Mridul Ghosh, Krishana Gulla, Sijy O’Dell, Joseph Varriale, Nathan Barefoot, Anita Changela, Cara W. Chao, Cheng Cheng, Aliaksandr Druz, Rui Kong, Krisha McKee, Reda Rawi, Edward K. Sarfo, Arne Schön, Andrew Shaddeau, Yaroslav Tsybovsky, Raffaello Verardi, Shuishu Wang, Timothy G. Wanninger, Kai Xu, Gengcheng J. Yang, Baoshan Zhang, Yaqiu Zhang, Tongqing Zhou, The VRC Production Program, Frank J. Arnold, Nicole A. Doria-Rose, Q. Paula Lei, Edward T. Ryan, Willie F. Vann, John R. Mascola & Peter D. Kwong. Scientific Reports . 2020. Article number: 3032 (2020). DOI: 10.1038/s41598-020-59711-y

DNA‐Mediated Assembly of Multispecific Antibodies for T Cell Engaging and Tumor Killing. Liqiang Pan, Chan Cao, Changqing Run, Liujuan Zhou, James J. Cho. Advanced Science. 2020. Volume7, Issue2 January 22, 2020, 1900973. November 23, 2019. DOI: 10.1002/advs.201900973

Rapid Gel Card Agglutination Assays for Serological Analysis Following SARS-CoV-2 Infection in Humans. Diana Alves, Rodrigo Curvello, Edward Henderson, Vidhishri Kesarwani, Julia A. Walker, Samuel C. Leguizamon, Heather McLiesh, Vikram Singh Raghuwanshi, Hajar Samadian, Erica M. Wood, Zoe K. McQuilten, Maryza Graham, Megan Wieringa, Tony M. Korman, Timothy F. Scott, Mark M. Banaszak Holl, Gil Garnier, and Simon R. Corrie. ACS Sens. 2020, 5, 8, 2596–2603. Publication Date:July 16, 2020 https://doi.org/10.1021/acssensors.0c01050

Visualizing multi-protein patterns at the synapse of neuronal tissue with DNA-assisted single-molecule localization microscopy. Kaarjel K. Narayanasamy, Aleksandar Stojic, Yunqing Li, Steffen Sass, Marina Hesse, Nina S. Deussner-Helfmann, Marina S. Dietz, Maja Klevanski, Thomas Kuner, Mike Heilemann. bioRxiv. 2021. DOI: 10.1101/2021.02.23.432306

Investigations into the Ice Crystallization and Freezing Properties of the Antifreeze Protein ApAFP752. Asenath-Smith, Emily Jeng, Emily C. Ambrogi, Emma K. Hoch, Garrett R. Olivier, Jason L., Engineer Research and Development Center (U.S.), ERDC/CRREL TR-22-17, 09/01/2022, 10.21079/11681/45620

The post MAL-dPEG®₂-NHS ester appeared first on VectorLabs.

]]> MAL-dPEG®₂-acid https://staging.vectorlabs.com/products/mal-dpeg2-acid/ Sat, 24 Feb 2024 02:07:46 +0000 https://staging.vectorlabs.com/?post_type=product&p=35660 MAL-dPEG®2-acid, product number QBD-10265, is a crosslinker that joins sulfhydryl groups to free amines through a single molecular weight polyethylene glycol (PEG) chain of discrete length (dPEG®). The maleimidopropyl moiety reacts with sulfhydryl groups via a Michael addition reaction. The propionic acid terminus of the molecule reacts directly with free amines using EDC or another carbodiimide to form amide bonds. Alternatively, activation of the propionic acid group with N-hydroxysuccinimide (NHS), 2,3,5,6-tetrafluorophenol (TFP), or some other acylating agent permits amide bond formation under mild, controlled conditions.

Specifications

Unit Size 100 mg, 1000 mg
Molecular Weight 328.32; single compound
Chemical formula C₁₄H₂₀N₂O₇
CAS 756525-98-1
Purity > 98%
Spacers dPEG® Spacer is 16 atoms and 17.5 Å
Shipping Ambient
Typical solubility properties (for additional information contact Customer Support) Methylene chloride, Acetonitrile, DMAC or DMSO.
Storage and handling -20°C; Always let come to room temperature before opening; be careful to limit exposure to moisture and restore under an inert atmosphere; stock solutions can be prepared with dry solvent and kept for several days (freeze when not in use). dPEG® pegylation compounds are generally hygroscopic and should be treated as such. This will be less noticeable with liquids, but the solids will become tacky and difficult to manipulate, if care is not taken to minimize air exposure.

The post MAL-dPEG®₂-acid appeared first on VectorLabs.

]]>

Description

MAL-dPEG®2-acid, product number QBD-10265, is a crosslinker that joins sulfhydryl groups to free amines through a single molecular weight polyethylene glycol (PEG) chain of discrete length (dPEG®). The maleimidopropyl moiety reacts with sulfhydryl groups via a Michael addition reaction. The propionic acid terminus of the molecule reacts directly with free amines using EDC or another carbodiimide to form amide bonds. Alternatively, activation of the propionic acid group with N-hydroxysuccinimide (NHS), 2,3,5,6-tetrafluorophenol (TFP), or some other acylating agent permits amide bond formation under mild, controlled conditions.

Specifications

Unit Size100 mg, 1000 mg
Molecular Weight328.32; single compound
Chemical formulaC₁₄H₂₀N₂O₇
CAS756525-98-1
Purity> 98%
SpacersdPEG® Spacer is 16 atoms and 17.5 Å
ShippingAmbient
Typical solubility properties (for additional information contact Customer Support)Methylene chloride, Acetonitrile, DMAC or DMSO.
Storage and handling-20°C; Always let come to room temperature before opening; be careful to limit exposure to moisture and restore under an inert atmosphere; stock solutions can be prepared with dry solvent and kept for several days (freeze when not in use). dPEG® pegylation compounds are generally hygroscopic and should be treated as such. This will be less noticeable with liquids, but the solids will become tacky and difficult to manipulate, if care is not taken to minimize air exposure.

Documents

References

Greg T. Hermanson, Bioconjugate Techniques, 2nd Edition, Elsevier Inc., Burlington, MA 01803, April, 2008 (ISBN-13: 978-0-12-370501-3; ISBN-10: 0-12-370501-0); See pp. 276-335 for general description and use of heterobifunctional crosslinkers, and the specific sample protocol for SMCC or sulfo-SMCC on pp. 283-286.

Greg T. Hermanson, Bioconjugate Techniques, 3rd Edition, Elsevier, Waltham, MA 02451, 2013, ISBN 978-0-12-382239-0; See Chapter 18, Discrete PEG Reagents, pp. 787-821, for a full overview of the dPEG® products.

Synthesis and anti-HIV activity of trivalent CD4-mimetic miniproteins. Hengguang Li, Yongjun Guan, Agnieszka Szczepanska, Antonio J. Moreno-Vargas, Ana T. Carmona, Inmaculada Robina, George K. Lewis and Lai-Xi Wang. Bioorganic & Medicinal Chemistry. 2007, 15 (12) pp 4220–4228. June 15, 2007. doi.org/10.1016/j.bmc.2007.03.064.

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]]> Amino-dPEG®₂-t-butyl ester https://staging.vectorlabs.com/products/amino-dpeg2-t-butyl-ester/ Sat, 24 Feb 2024 02:07:45 +0000 https://staging.vectorlabs.com/?post_type=product&p=35652 Amino-dPEG®2–t-butyl ester, product number QBD-10264, is a monodispersed polyethylene glycol (PEG) crosslinker containing a primary amino group and a tert-butyl ester-protected propionic acid group. The terminal amine reacts with carboxylates to form amide bonds and with aldehydes and ketones to form semi-labile, reducible Schiff bases. The tert-butyl ester protecting group removes easily with trifluoroacetic acid (TFA), leaving the propionic acid free to react with amines or form an active ester with N-hydroxysuccinimide (NHS) or 2,3,5,6-tetrafluorophenol (TFP). This compound is useful as a building block in supramolecular construction, a peptide modification reagent, a surface coating for biomolecules and inorganic/metal surfaces, or for protein modification.

Specifications

Unit Size 100 mg, 1000 mg
Molecular Weight 233.30; single compound
Chemical formula C₁₁H₂₃NO₄
CAS 756525-95-8
Purity > 98%
Spacers dPEG® Spacer is 10 atoms and 10.9 Å
Shipping Ambient
Typical solubility properties (for additional information contact Customer Support) Methylene chloride, DMAC, or DMSO.
Storage and handling -20°C; Always let come to room temperature before opening; be careful to limit exposure to moisture and restore under an inert atmosphere; stock solutions can be prepared with dry solvent and kept for several days (freeze when not in use). dPEG® pegylation compounds are generally hygroscopic and should be treated as such. This will be less noticeable with liquids, but the solids will become tacky and difficult to manipulate, if care is not taken to minimize air exposure.

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Description

Amino-dPEG®2–t-butyl ester, product number QBD-10264, is a monodispersed polyethylene glycol (PEG) crosslinker containing a primary amino group and a tert-butyl ester-protected propionic acid group. The terminal amine reacts with carboxylates to form amide bonds and with aldehydes and ketones to form semi-labile, reducible Schiff bases. The tert-butyl ester protecting group removes easily with trifluoroacetic acid (TFA), leaving the propionic acid free to react with amines or form an active ester with N-hydroxysuccinimide (NHS) or 2,3,5,6-tetrafluorophenol (TFP). This compound is useful as a building block in supramolecular construction, a peptide modification reagent, a surface coating for biomolecules and inorganic/metal surfaces, or for protein modification.

Specifications

Unit Size100 mg, 1000 mg
Molecular Weight233.30; single compound
Chemical formulaC₁₁H₂₃NO₄
CAS756525-95-8
Purity> 98%
SpacersdPEG® Spacer is 10 atoms and 10.9 Å
ShippingAmbient
Typical solubility properties (for additional information contact Customer Support)Methylene chloride, DMAC, or DMSO.
Storage and handling-20°C; Always let come to room temperature before opening; be careful to limit exposure to moisture and restore under an inert atmosphere; stock solutions can be prepared with dry solvent and kept for several days (freeze when not in use). dPEG® pegylation compounds are generally hygroscopic and should be treated as such. This will be less noticeable with liquids, but the solids will become tacky and difficult to manipulate, if care is not taken to minimize air exposure.

Documents

References

Greg T. Hermanson, Bioconjugate Techniques, 3rd Edition, Elsevier, Waltham, MA 02451, 2013, ISBN 978-0-12-382239-0; See Chapter 18, Discrete PEG Reagents, pp. 787-821, for a full overview of the dPEG® products.

The post Amino-dPEG®₂-t-butyl ester appeared first on VectorLabs.

]]> Fmoc-N-amido-dPEG®₂-acid https://staging.vectorlabs.com/products/fmoc-n-amido-dpeg2-acid/ Sat, 24 Feb 2024 02:07:32 +0000 https://staging.vectorlabs.com/?post_type=product&p=35559 Fmoc-N-amido-dPEG®2-acid, product number QBD-10243, is one of a broad line of products designed for use in peptide synthesis. The short (16 atoms), discrete PEG (dPEG®) spacer is functionalized with a propionic acid group on one end and Fmoc-protected amine on the other. The product can be added to the N-terminus of a growing peptide chain or to a primary-amine-functionalized side chain of an amino acid such as lysine. The dPEG®2 spacer imparts water solubility to the peptide to which it is conjugated.

Description

Fmoc-N-amido-dPEG®2-acid, product number QBD-10243, is a peptide synthesis product used to introduce a short (10 atoms), hydrophilic spacer into a peptide chain using standard peptide chemistry. The Fmoc protecting group can be removed using standard peptide chemistry.

Fmoc-N-amido-dPEG®2-acid, QBD-10243, works well in solid-phase and solution-phase synthetic processes. The dPEG® can be joined to the N-terminal end of a peptide chain. After removal of the Fmoc protecting group, further synthesis can be carried out to create a peptide with a flexible hinge. The peptide sequences on either side of the dPEG® spacer can be identical or different.

In addition, the short dPEG® linker can provide spacing between two units in a synthetic construct where steric hindrance is a problem. The amphiphilic nature of dPEG® means that the construct will gain water solubility while remaining soluble in an organic solvent. The Fmoc protecting group removes easily with a solution of piperidine in N,N-dimethylformamide (DMF).

Specifications

Unit Size 1000 mg
Molecular Weight 399.44; single compound
Chemical formula C₂₂H₂₅NO₆
CAS 872679-70-4
Purity > 98%
Spacers dPEG® Spacer is 10 atoms and 10.9 Å
Shipping Ambient
Typical solubility properties (for additional information contact Customer Support) Methylene chloride, Acetontrile, DMAC or DMSO.
Storage and handling -20°C; Always let come to room temperature before opening; be careful to limit exposure to moisture and restore under an inert atmosphere; stock solutions can be prepared with dry solvent and kept for several days (freeze when not in use). dPEG® pegylation compounds are generally hygroscopic and should be treated as such. This will be less noticeable with liquids, but the solids will become tacky and difficult to manipulate, if care is not taken to minimize air exposure.

The post Fmoc-N-amido-dPEG®₂-acid appeared first on VectorLabs.

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Fmoc-N-amido-dPEG®2-acid, product number QBD-10243, is one of a broad line of products designed for use in peptide synthesis. The short (16 atoms), discrete PEG (dPEG®) spacer is functionalized with a propionic acid group on one end and Fmoc-protected amine on the other. The product can be added to the N-terminus of a growing peptide chain or to a primary-amine-functionalized side chain of an amino acid such as lysine. The dPEG®2 spacer imparts water solubility to the peptide to which it is conjugated.

Description

Fmoc-N-amido-dPEG®2-acid, product number QBD-10243, is a peptide synthesis product used to introduce a short (10 atoms), hydrophilic spacer into a peptide chain using standard peptide chemistry. The Fmoc protecting group can be removed using standard peptide chemistry.

Fmoc-N-amido-dPEG®2-acid, QBD-10243, works well in solid-phase and solution-phase synthetic processes. The dPEG® can be joined to the N-terminal end of a peptide chain. After removal of the Fmoc protecting group, further synthesis can be carried out to create a peptide with a flexible hinge. The peptide sequences on either side of the dPEG® spacer can be identical or different.

In addition, the short dPEG® linker can provide spacing between two units in a synthetic construct where steric hindrance is a problem. The amphiphilic nature of dPEG® means that the construct will gain water solubility while remaining soluble in an organic solvent. The Fmoc protecting group removes easily with a solution of piperidine in N,N-dimethylformamide (DMF).

Specifications

Unit Size1000 mg
Molecular Weight399.44; single compound
Chemical formulaC₂₂H₂₅NO₆
CAS872679-70-4
Purity> 98%
SpacersdPEG® Spacer is 10 atoms and 10.9 Å
ShippingAmbient
Typical solubility properties (for additional information contact Customer Support)Methylene chloride, Acetontrile, DMAC or DMSO.
Storage and handling-20°C; Always let come to room temperature before opening; be careful to limit exposure to moisture and restore under an inert atmosphere; stock solutions can be prepared with dry solvent and kept for several days (freeze when not in use). dPEG® pegylation compounds are generally hygroscopic and should be treated as such. This will be less noticeable with liquids, but the solids will become tacky and difficult to manipulate, if care is not taken to minimize air exposure.

Documents

References

Greg T. Hermanson, Bioconjugate Techniques, 3rd Edition, Elsevier, Waltham, MA 02451, 2013, ISBN 978-0-12-382239-0; See Chapter 18, Discrete PEG Reagents, pp. 787-821, for a full overview of the dPEG® products.

Functional PEG-Modified Thin Films for Biological Detection. Aaron S. Anderson, Andrew M. Dattelbaum, Gabriel A. Montano, Dominique N. Price, Jurgen G. Schmidt, Jennifer S. Martinez, W. Kevin Grace, Karen M. Grace, and Basil I. Swanson. Langmuir. 2008, 24 (5), pp 2240–2247. January 30, 2008. DOI: 10.1021/la7033438.

Polyproline-Rod Approach to Isolating Protein Targets of Bioactive Small Molecules: Isolation of a New Target of Indomethacin. Shin-ichi Sato, Youngjoo Kwon, Shinji Kamisuki, Neeta Srivastava, Quian Mao, Yoshinori Kawazoe, and Motonari Uesugi. J. Am. Chem. Soc. 2007, 129 (4), pp 873–880. January 4, 2007. DOI: 10.1021/ja0655643.

Oriented Surface Immobilization of Antibodies at the Conserved Nucleotide Binding Site for Enhanced Antigen Detection. Nathan J Alves, Tanyel Kiziltepe, and Basar Bilgicer. Langmuir. 2012, 28 (25) pp 9640-9648. May 21, 2012. DOI: 10.1021/la301887s.

Enhanced Cellular Uptake of Peptide-Targeted Nanoparticles through Increased Peptide Hydrophilicity and Optimized Ethylene Glycol Peptide-Linker Length. Jared F. Stefanick, Jonathan D. Ashley, and Basar Bilgicer. ACS Nano. 2013, 7 (9) pp 8115–8127. August 29, 2013. DOI: 10.1021/nn4033954.

Selective photocrosslinking of functional ligands to antibodies via the conserved nucleotide binding site. Nathan J. Alves, Matthew M. Champion, Jared F. Stefanick, Michael W. Handlogten, Demetri T. Moustakas, Yunhua Shi, Bryan F. Shawd, Rudolph M. Navari, Tanyel Kiziltepe, Basar Bilgicer. Biomaterials. 2013, 34 (22) pp 5700–5710. April 16, 2013. DOI:.org/10.1016/j.biomaterials.2013.03.082.

Design, Synthesis and Evaluation of a Neurokinin 1 Receptor-Targeted Near IR Dye for Fluorescence Guided Surgery of Neuroendocrine Cancers. Ananda Kumar Kanduluru, Madduri Srinivasarao, and Philip S. Low. Bioconjugate Chemistry. 2016, pp 1-20. August 16, 2016. DOI: 10.1021/acs.bioconjchem.6b00374.

One-Pot Isolation of a Desired Human Genome Fragment by Using a Biotinylated pcPNA/S1 Nuclease Combination. Arivazhagan Rajendran , Narumi Shigi, Jun Sumaoka, and Makoto Komiyama. Biochemistry. 2018, 57, pp 2908-2912. May 3, 2018. DOI: 10.1021/acs.biochem.8b00202.

Stapled, Long-Acting Glucagon-like Peptide 2 Analog with Efficacy in Dextran Sodium Sulfate Induced Mouse Colitis Models. Peng-Yu Yang , Huafei Zou, Candy Lee, Avinash Muppidi, Elizabeth Chao, Qiangwei Fu, Xiaozhou Luo, Danling Wang, Peter G. Schultz, and Weijun Shen. Journal of Medicinial Chemistry. 2018, 61, pp 3218-3223. March 12, 2018. DOI: 10.1021/acs.jmedchem.7b00768.

Evaluation of the Pharmacokinetic Effects of Various Linking Group Using the 111In-DOTA-X-BBN(7−14)NH2 Structural Paradigm in a Prostate Cancer Model. Jered C. Garrison, Tammy L. Rold, Gary L. Sieckman, Farah Naz, Samantha V. Sublett, Said Daibes Figueroa, Wynn A. Volkert and Timothy J. Hoffman. Bioconjugate Chem. 2008, 19 (9) pp 1803–1812. August 20, 2008. DOI: 10.1021/bc8001375.

PEG-Peptide Conjugates. Ian W Hamley. Biomacromolecules. 2014. April 1, 2014. DOI: 10.1021/bm500246w.

Dual-receptor targeted strategy in nanoparticle design achieves tumor cell selectivity through cooperativity. Jared Francis Stefanick, David Thomas Omstead, Tanyel Kiziltepeabc and Basar Bilgicer. Nanoscale. 2019. Fenruary 17, 2019. DOI: 10.1039/C8NR09431D

Designer covalent heterobivalent inhibitors prevent IgE-dependent responses to peanut allergen. Peter E. Deak, Baksun Kim, Amina Abdul Qayum, Jaeho Shin, Girish Vitalpur, Kirsten M. Kloepfer, Matthew J. Turner, Neal Smith, Wayne G. Shreffler, Tanyel Kiziltepe, Mark H. Kaplan, and Basar Bilgicer. 2019. April 8, 2019. DOI: 10.1073/pnas.1820417116

Membrane-Fusogen Distance Is Critical for Efficient Coiled-Coil-Peptide-Mediated Liposome Fusion. Geert A. Daudey, Harshal R. Zope, Jens Voskuhl, Alexander Kros , and Aimee L. Boyle. Langmuir. 2017, 33 (43) pp 12443-12452. 10/5/2017. DOI: 10.1021/acs.langmuir.7b02931.

Design and Evaluation of Novel Polymyxin Fluorescent Probes. Bo Yun , Kade D. Roberts, Philip E. Thompson, Roger L. Nation, Tony Velkov, and Jian Li. Sensors. 2017, 17 (11) pp 2598. 11/11/2017. DOI: 10.3390/s17112598.

Engineering peptide-targeted liposomal nanoparticles optimized for improved selectivity for HER2-positive breast cancer cells to achieve enhanced in vivo efficacy. Baksun Kim, Jaeho Shin, Junmin Wu, David T. Omstead, Tanyel Kiziltepe, Laurie E. Littlepage, Basar Bilgicer. Journal of Controlled Release. 2020. Volume 322, 10 June 2020, Pages 530-541. April 8, 2020. DOI: 10.1016/j.jconrel.2020.04.010

In vivo evaluation of CD38 and CD138 as targets for nanoparticle-based drug delivery in multiple myeloma. David T. Omstead, Franklin Mejia, Jenna Sjoerdsma, Baksun Kim, Jaeho Shin, Sabrina Khan, Junmin Wu, Tanyel Kiziltepe, Laurie E. Littlepage & Basar Bilgicer. Journal of Hematology & Oncology. 2020. 13, Article number: 145 (2020). 11/02/20. DOI: 10.1186/s13045-020-00965-4

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