Azide – VectorLabs https://staging.vectorlabs.com From linker design and synthesis, bioconjugated design and manufacturing, biomolecule labeling and functionalization, and detection system and solutions, our team is here to help you move forward, with impact. Wed, 22 Oct 2025 17:20:50 +0000 en-US hourly 1 https://wordpress.org/?v=6.9 https://staging.vectorlabs.com/wp-content/uploads/2024/11/V-favicon-02-100x100.png Azide – VectorLabs https://staging.vectorlabs.com 32 32 Bromoacetamido-dPEG®₃-azide https://staging.vectorlabs.com/products/bromoacetamido-dpeg3-azide/ Sat, 24 Feb 2024 02:15:21 +0000 https://staging.vectorlabs.com/?post_type=product&p=39306 Bromoacetamido-dPEG®3-azide, product number QBD-11217, is discrete PEG (dPEG®) crosslinker that facilitates the crosslinking of free thiols with click chemistry alkyne partners across a short (16 atoms, 17.5 Å) single molecular weight PEG bridge.

The short, amphiphilic dPEG® linker adds hydrodynamic volume to conjugate molecules and increases their water solubility. The flexible, non-immunogenic spacer can reduce or eliminate renal clearance and help shield conjugates from opsonization through the increased hydrodynamic volume the dPEG® linker imparts.

The bromoacetate moiety reacts chemoselectively with free thiols at pH >8.0. It is an alternative to the maleimide group and is used in situations where the target molecule needs to be at a high pH. The azide group reacts with a suitable alkyne partner via metal-catalyzed (Cu, Ru) or strain-promoted (copper-free) click chemistry. Moreover, Bromoacetamido-dPEG®3-azide is stable, unlike MAL-PEG-azide constructs, which are somewhat unstable.

Specifications

Unit Size 100 mg, 1000 mg
Molecular Weight 339.19; single compound
Chemical formula C₁₀H₁₉BrN₄O₄
CAS N/A
Purity > 98%
Spacers dPEG® Spacer is 16 atoms and 17.5 Å
Shipping Ambient
Typical solubility properties (for additional information contact Customer Support) Methylene Chloride, Methanol, Acetone, Acetonitrile, DMF, or DMSO.
Storage and handling -20°C; Always let come to room temperature before opening; be careful to limit exposure to moisture and restore under an inert atmosphere; stock solutions can be prepared with dry solvent and kept for several days (freeze when not in use). dPEG® pegylation compounds are generally hygroscopic and should be treated as such. This will be less noticeable with liquids, but the solids will become tacky and difficult to manipulate, if care is not taken to minimize air exposure.

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Description

Bromoacetamido-dPEG®3-azide, product number QBD-11217, is discrete PEG (dPEG®) crosslinker that facilitates the crosslinking of free thiols with click chemistry alkyne partners across a short (16 atoms, 17.5 Å) single molecular weight PEG bridge.

The short, amphiphilic dPEG® linker adds hydrodynamic volume to conjugate molecules and increases their water solubility. The flexible, non-immunogenic spacer can reduce or eliminate renal clearance and help shield conjugates from opsonization through the increased hydrodynamic volume the dPEG® linker imparts.

The bromoacetate moiety reacts chemoselectively with free thiols at pH >8.0. It is an alternative to the maleimide group and is used in situations where the target molecule needs to be at a high pH. The azide group reacts with a suitable alkyne partner via metal-catalyzed (Cu, Ru) or strain-promoted (copper-free) click chemistry. Moreover, Bromoacetamido-dPEG®3-azide is stable, unlike MAL-PEG-azide constructs, which are somewhat unstable.

Specifications

Unit Size100 mg, 1000 mg
Molecular Weight339.19; single compound
Chemical formulaC₁₀H₁₉BrN₄O₄
CASN/A
Purity> 98%
SpacersdPEG® Spacer is 16 atoms and 17.5 Å
ShippingAmbient
Typical solubility properties (for additional information contact Customer Support)Methylene Chloride, Methanol, Acetone, Acetonitrile, DMF, or DMSO.
Storage and handling-20°C; Always let come to room temperature before opening; be careful to limit exposure to moisture and restore under an inert atmosphere; stock solutions can be prepared with dry solvent and kept for several days (freeze when not in use). dPEG® pegylation compounds are generally hygroscopic and should be treated as such. This will be less noticeable with liquids, but the solids will become tacky and difficult to manipulate, if care is not taken to minimize air exposure.



Documents

References

Greg T. Hermanson, Bioconjugate Techniques, 2nd Edition, Elsevier Inc., Burlington, MA 01803, April, 2008 (ISBN-13: 978-0-12-370501-3; ISBN-10: 0-12-370501-0). Specifically see pp. 726-729 in his Chapter 18 on discrete PEG compounds for pegylation applications.

Greg T. Hermanson, Bioconjugate Techniques, 3rd Edition, Elsevier, Waltham, MA 02451, 2013, ISBN 978-0-12-382239-0; See chapter 18, Discrete PEG Reagents, pp.787-821, for a full overview of the dPEG® products.

The post Bromoacetamido-dPEG®₃-azide appeared first on VectorLabs.

]]> Bromoacetamido-dPEG®₂₃-azide https://staging.vectorlabs.com/products/bromoacetamido-dpeg23-azide/ Sat, 24 Feb 2024 02:15:20 +0000 https://staging.vectorlabs.com/?post_type=product&p=39298 Bromoacetamido-dPEG®23-azide, product number QBD-11205, is discrete PEG (dPEG®) crosslinker that facilitates the crosslinking of free thiols with click chemistry alkyne partners across a long (76 atoms, 88.5 Å) single molecular weight PEG bridge.

The long, amphiphilic dPEG® linker adds hydrodynamic volume to conjugate molecules and increases their water solubility. The flexible, non-immunogenic spacer reduces or eliminates renal clearance and helps shield conjugates from opsonization through the increased hydrodynamic volume the dPEG® linker imparts.

The bromoacetate moiety reacts chemoselectively with free thiols at pH >8.0. It is an alternative to the maleimide group and is used in situations where the target molecule needs to be at a high pH. The azide group reacts with a suitable alkyne partner via metal-catalyzed (Cu, Ru) or strain-promoted (copper-free) click chemistry. Moreover, Bromoacetamido-dPEG®23-azide is stable, unlike MAL-PEG-azide constructs, which are somewhat unstable.

Specifications

Unit Size 100 mg, 1000 mg
Molecular Weight 1220.24; single compound
Chemical formula C₅₀H₉₉BrN₄O₂₄
CAS N/A
Purity > 98%
Spacers dPEG® Spacer is 76 atoms and 88.5 Å
Shipping Ambient
Typical solubility properties (for additional information contact Customer Support) Methylene Chloride, Methanol, Acetone, Chloroform, DMSO, DMAC, or DMF.
Storage and handling -20°C; Always let come to room temperature before opening; be careful to limit exposure to moisture and restore under an inert atmosphere; stock solutions can be prepared with dry solvent and kept for several days (freeze when not in use). dPEG® pegylation compounds are generally hygroscopic and should be treated as such. This will be less noticeable with liquids, but the solids will become tacky and difficult to manipulate, if care is not taken to minimize air exposure.

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Description

Bromoacetamido-dPEG®23-azide, product number QBD-11205, is discrete PEG (dPEG®) crosslinker that facilitates the crosslinking of free thiols with click chemistry alkyne partners across a long (76 atoms, 88.5 Å) single molecular weight PEG bridge.

The long, amphiphilic dPEG® linker adds hydrodynamic volume to conjugate molecules and increases their water solubility. The flexible, non-immunogenic spacer reduces or eliminates renal clearance and helps shield conjugates from opsonization through the increased hydrodynamic volume the dPEG® linker imparts.

The bromoacetate moiety reacts chemoselectively with free thiols at pH >8.0. It is an alternative to the maleimide group and is used in situations where the target molecule needs to be at a high pH. The azide group reacts with a suitable alkyne partner via metal-catalyzed (Cu, Ru) or strain-promoted (copper-free) click chemistry. Moreover, Bromoacetamido-dPEG®23-azide is stable, unlike MAL-PEG-azide constructs, which are somewhat unstable.

Specifications

Unit Size100 mg, 1000 mg
Molecular Weight1220.24; single compound
Chemical formulaC₅₀H₉₉BrN₄O₂₄
CASN/A
Purity> 98%
SpacersdPEG® Spacer is 76 atoms and 88.5 Å
ShippingAmbient
Typical solubility properties (for additional information contact Customer Support)Methylene Chloride, Methanol, Acetone, Chloroform, DMSO, DMAC, or DMF.
Storage and handling-20°C; Always let come to room temperature before opening; be careful to limit exposure to moisture and restore under an inert atmosphere; stock solutions can be prepared with dry solvent and kept for several days (freeze when not in use). dPEG® pegylation compounds are generally hygroscopic and should be treated as such. This will be less noticeable with liquids, but the solids will become tacky and difficult to manipulate, if care is not taken to minimize air exposure.



Documents

References

Greg T. Hermanson, Bioconjugate Techniques, 2nd Edition, Elsevier Inc., Burlington, MA 01803, April, 2008 (ISBN-13: 978-0-12-370501-3; ISBN-10: 0-12-370501-0). Specifically see pp. 726-729 in his Chapter 18 on discrete PEG compounds for pegylation applications.

Greg T. Hermanson, Bioconjugate Techniques, 3rd Edition, Elsevier, Waltham, MA 02451, 2013, ISBN 978-0-12-382239-0; See chapter 18, Discrete PEG Reagents, pp.787-821, for a full overview of the dPEG® products.

The post Bromoacetamido-dPEG®₂₃-azide appeared first on VectorLabs.

]]> Bromoacetamido-dPEG®₁₁-azide https://staging.vectorlabs.com/products/bromoacetamido-dpeg11-azide/ Sat, 24 Feb 2024 02:15:19 +0000 https://staging.vectorlabs.com/?post_type=product&p=39290 Bromoacetamido-dPEG®11-azide, product number QBD-11204, crosslinks free thiols with click chemistry alkyne partners across a medium-length (39 atoms, 46.0 Å) single molecular weight, discrete PEG (dPEG®) bridge.

The medium-length dPEG® linker is amphiphilic and adds hydrodynamic volume and water solubility to conjugate molecules. The flexible, non-immunogenic spacer can reduce or eliminate renal clearance and help shield conjugates from opsonization through the increased hydrodynamic volume the dPEG® linker imparts.

The bromoacetate moiety reacts chemoselectively with free thiols at pH >8.0. The bromoacetate group is an alternative to the maleimide group and is used in situations where the target molecule needs to be at a high pH. The azide group reacts with a suitable alkyne partner via metal-catalyzed (Cu, Ru) or strain-promoted (copper-free) click chemistry. Moreover, Bromoacetamido-dPEG®11-azide is stable, unlike MAL-PEG-azide constructs, which are somewhat unstable.

Specifications

Unit Size 100 mg, 1000 mg
Molecular Weight 691.61; single compound
Chemical formula C₂₆H₅₁BrN₄O₁₂
CAS N/A
Purity > 98%
Spacers dPEG® Spacer is 39 atoms and 46.0 Å
Shipping Ambient
Typical solubility properties (for additional information contact Customer Support) Methylene Chloride, DMAC, DMF, and DMSO
Storage and handling -20°C; Always let come to room temperature before opening; be careful to limit exposure to moisture and restore under an inert atmosphere; stock solutions can be prepared with dry solvent and kept for several days (freeze when not in use). dPEG® pegylation compounds are generally hygroscopic and should be treated as such. This will be less noticeable with liquids, but the solids will become tacky and difficult to manipulate, if care is not taken to minimize air exposure.

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Description

Bromoacetamido-dPEG®11-azide, product number QBD-11204, crosslinks free thiols with click chemistry alkyne partners across a medium-length (39 atoms, 46.0 Å) single molecular weight, discrete PEG (dPEG®) bridge.

The medium-length dPEG® linker is amphiphilic and adds hydrodynamic volume and water solubility to conjugate molecules. The flexible, non-immunogenic spacer can reduce or eliminate renal clearance and help shield conjugates from opsonization through the increased hydrodynamic volume the dPEG® linker imparts.

The bromoacetate moiety reacts chemoselectively with free thiols at pH >8.0. The bromoacetate group is an alternative to the maleimide group and is used in situations where the target molecule needs to be at a high pH. The azide group reacts with a suitable alkyne partner via metal-catalyzed (Cu, Ru) or strain-promoted (copper-free) click chemistry. Moreover, Bromoacetamido-dPEG®11-azide is stable, unlike MAL-PEG-azide constructs, which are somewhat unstable.

Specifications

Unit Size100 mg, 1000 mg
Molecular Weight691.61; single compound
Chemical formulaC₂₆H₅₁BrN₄O₁₂
CASN/A
Purity> 98%
SpacersdPEG® Spacer is 39 atoms and 46.0 Å
ShippingAmbient
Typical solubility properties (for additional information contact Customer Support)Methylene Chloride, DMAC, DMF, and DMSO
Storage and handling-20°C; Always let come to room temperature before opening; be careful to limit exposure to moisture and restore under an inert atmosphere; stock solutions can be prepared with dry solvent and kept for several days (freeze when not in use). dPEG® pegylation compounds are generally hygroscopic and should be treated as such. This will be less noticeable with liquids, but the solids will become tacky and difficult to manipulate, if care is not taken to minimize air exposure.



Documents

References

Greg T. Hermanson, Bioconjugate Techniques, 3rd Edition, Elsevier, Waltham, MA 02451, 2013, ISBN 978-0-12-382239-0; See chapter 18, Discrete PEG Reagents, pp.787-821, for a full overview of the dPEG® products.

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]]> Aminooxy-dPEG®₁₁-azide.HCl https://staging.vectorlabs.com/products/aminooxy-dpeg11-azide-hcl/ Sat, 24 Feb 2024 02:11:27 +0000 https://staging.vectorlabs.com/?post_type=product&p=36370 Aminooxy-dPEG®11-azide.HCl, product number QBD-10538, is a click chemistry crosslinker functionalized with an oxyamine moiety on one end of a medium-length (38 atoms), single molecular weight, discrete PEG (dPEG®) linker, and an azide group on the other end. The azide end of the linker reacts with aliphatic or cyclic alkynes through copper-catalyzed (CuAAC), ruthenium-catalyzed (RuAAC), or strain-promoted, copper-free (SPAAC) click chemistry to form a triazole ring. The molecule’s aminooxy end (sold as an HCl salt) reacts with aldehydes and ketones to form biocompatible, stable oxime bonds that are important in many bioconjugation and supramolecular construction processes.

Specifications

Unit Size 50 mg, 1000 mg
Molecular Weight 623.134
Chemical formula C₂₄H₅₁CIN₄O₁₂
CAS N/A
Purity > 95%
Spacers dPEG® Spacer is 38 atoms and 40.8Å
Shipping Ambient
Typical solubility properties (for additional information contact Customer Support) Water, DCM, or methanol
Storage and handling -20°C; Always let come to room temperature before opening; be careful to limit exposure to moisture and restore under an inert atmosphere; stock solutions can be prepared with dry solvent and kept for several days (freeze when not in use). dPEG® pegylation compounds are generally hygroscopic and should be treated as such. This will be less noticeable with liquids, but the solids will become tacky and difficult to manipulate, if care is not taken to minimize air exposure.

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Description

Aminooxy-dPEG®11-azide.HCl, product number QBD-10538, is a click chemistry crosslinker functionalized with an oxyamine moiety on one end of a medium-length (38 atoms), single molecular weight, discrete PEG (dPEG®) linker, and an azide group on the other end. The azide end of the linker reacts with aliphatic or cyclic alkynes through copper-catalyzed (CuAAC), ruthenium-catalyzed (RuAAC), or strain-promoted, copper-free (SPAAC) click chemistry to form a triazole ring. The molecule’s aminooxy end (sold as an HCl salt) reacts with aldehydes and ketones to form biocompatible, stable oxime bonds that are important in many bioconjugation and supramolecular construction processes.

Specifications

Unit Size50 mg, 1000 mg
Molecular Weight623.134
Chemical formulaC₂₄H₅₁CIN₄O₁₂
CASN/A
Purity> 95%
SpacersdPEG® Spacer is 38 atoms and 40.8Å
ShippingAmbient
Typical solubility properties (for additional information contact Customer Support)Water, DCM, or methanol
Storage and handling-20°C; Always let come to room temperature before opening; be careful to limit exposure to moisture and restore under an inert atmosphere; stock solutions can be prepared with dry solvent and kept for several days (freeze when not in use). dPEG® pegylation compounds are generally hygroscopic and should be treated as such. This will be less noticeable with liquids, but the solids will become tacky and difficult to manipulate, if care is not taken to minimize air exposure.



Documents

References

Greg T. Hermanson, Bioconjugate Techniques, 2nd Edition, Elsevier Inc., Burlington, MA 01803, April, 2008 (ISBN-13: 978-0-12-370501-3; ISBN-10: 0-12-370501-0). Specifically see pp. 726-729 in his chapter 18 on discrete PEG compounds.

Greg T. Hermanson, Bioconjugate Techniques, 3rd Edition, Elsevier, Waltham, MA 02451, 2013, ISBN 978-0-12-382239-0; See Chapter 18, Discrete PEG Reagents, pp. 787-821, for a full overview of the dPEG® products.

The post Aminooxy-dPEG®₁₁-azide.HCl appeared first on VectorLabs.

]]> Kdo Azide https://staging.vectorlabs.com/products/kdo-azide/ Tue, 19 Sep 2023 19:18:01 +0000 https://staging.vectorlabs.com/?post_type=product&p=21804 Description

8-Azido-3,8-dideoxy-D-manno-octulosonic acid (Kdo Azide) is an analogue of the natural 3-deoxy-D-manno-octulosonic acid (Kdo) that contains a very small modification, specifically an azido moiety. This molecule can be incorporated by cultured cells (Gram-negative Bacteria, for example) and incorporated into glycans (LPS, for example) during active glycan biosynthesis. Detection utilizes the chemoselective ligation or “click” reaction between an azide and an alkyne or cyclooctyne.

Specifications

Unit Size 10 mg, 25 mg, 100 mg
Molecular weight 280.24
Chemical composition C8H16N4O7
CAS 1380099-68-2
Solubility DMSO, DMF, Water
Purity >90%
Appearance White lyophilized powder
Storage Conditions -20°C.
Shipping Conditions Ambient temperature

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Description

8-Azido-3,8-dideoxy-D-manno-octulosonic acid (Kdo Azide) is an analogue of the natural 3-deoxy-D-manno-octulosonic acid (Kdo) that contains a very small modification, specifically an azido moiety. This molecule can be incorporated by cultured cells (Gram-negative Bacteria, for example) and incorporated into glycans (LPS, for example) during active glycan biosynthesis. Detection utilizes the chemoselective ligation or “click” reaction between an azide and an alkyne or cyclooctyne.

Specifications

Unit Size10 mg, 25 mg, 100 mg
Molecular weight280.24
Chemical compositionC8H16N4O7
CAS1380099-68-2
SolubilityDMSO, DMF, Water
Purity>90%
AppearanceWhite lyophilized powder
Storage Conditions-20°C.
Shipping ConditionsAmbient temperature

Documents

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]]> N-azidoacetylgalactosamine-tetraacylated (Ac4GalNAz) https://staging.vectorlabs.com/products/n-azidoacetylgalactosamine-tetraacylated-ac4galnaz/ Tue, 19 Sep 2023 19:18:00 +0000 https://staging.vectorlabs.com/?post_type=product&p=21800 Description

The unnatural azido-containing monosaccharide building block. The azide moiety can be used for modification though chemoselective ligation chemistries including CuAAC, Cu-free click reaction or Staudinger ligation. The acetyl groups increase solubility in many solvents and make handling of this reagent easier.

Specifications

Unit Size 5 mg, 25 mg, 100 mg
Molecular weight 430.37
Chemical composition C16H22N4O10
CAS 653600-56-7
Solubility DMSO, DMF, DCM, THF, Chloroform
Purity >90%
Appearance White to grey amorphous solid
Storage Conditions -20°C.
Shipping Conditions Ambient temperature

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Description

The unnatural azido-containing monosaccharide building block. The azide moiety can be used for modification though chemoselective ligation chemistries including CuAAC, Cu-free click reaction or Staudinger ligation. The acetyl groups increase solubility in many solvents and make handling of this reagent easier.

Specifications

Unit Size5 mg, 25 mg, 100 mg
Molecular weight430.37
Chemical compositionC16H22N4O10
CAS653600-56-7
SolubilityDMSO, DMF, DCM, THF, Chloroform
Purity>90%
AppearanceWhite to grey amorphous solid
Storage Conditions-20°C.
Shipping ConditionsAmbient temperature

Documents

Selected References

  1. Simon P. Wisnovsky, et al. (2020). Metabolic precision labeling enables selective probing of O-linked N-acetylgalactosamine glycosylation. PNAS, 117 (41), 25293-25301. [PNAS]
  2. Ranzinger, R., et al. (2020). Mass Spectrometric Method for the Unambiguous Profiling of Cellular Dynamic Glycosylation. ACS Chem. Biol., 15, 10, 2692-2701. [ACSPublications]
  3. Suttapitugsakul, S., et al. (2019). Surface Glycoproteomic Analysis Reveals That Both Unique and Differential Expression of Surface Glycoproteins Determine the Cell Type. Anal. Chem., 91(10), 6934–42. [PubMed]
 

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]]> N-azidoacetylglucosamine-tetraacylated (Ac4GlcNAz) https://staging.vectorlabs.com/products/n-azidoacetylglucosamine-tetraacylated-ac4glcnaz/ Tue, 19 Sep 2023 19:17:33 +0000 https://staging.vectorlabs.com/?post_type=product&p=21795 Description

The unnatural azido-containing monosaccharide building block. The azide moiety can be used for modification though chemoselective ligation chemistries including CuAAC, Cu-free click reaction or Staudinger ligation. The acetyl groups increase solubility in many solvents and make handling of this reagent easier.

Specifications

Unit Size 5 mg, 25 mg, 100 mg
Chemical composition C18H22N4O10
CAS
98924-81-3
Solubility DMSO, DMF, DCM, THF, Chloroform
Purity >90%
Appearance White to grey amorphous solid
Storage Conditions -20°C.
Shipping Conditions Ambient temperature

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Description

The unnatural azido-containing monosaccharide building block. The azide moiety can be used for modification though chemoselective ligation chemistries including CuAAC, Cu-free click reaction or Staudinger ligation. The acetyl groups increase solubility in many solvents and make handling of this reagent easier.

Specifications

Unit Size5 mg, 25 mg, 100 mg
Chemical compositionC18H22N4O10
CAS
98924-81-3
SolubilityDMSO, DMF, DCM, THF, Chloroform
Purity>90%
AppearanceWhite to grey amorphous solid
Storage Conditions-20°C.
Shipping ConditionsAmbient temperature

Documents

Selected References

  1. Ranzinger, R., et al. (2020). Mass Spectrometric Method for the Unambiguous Profiling of Cellular Dynamic Glycosylation. ACS Chem. Biol., 15, 10, 2692-2701. [ACSPublications]
 

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]]> N-azidoacetylmannosamine-tetraacylated (Ac4ManNAz) https://staging.vectorlabs.com/products/n-azidoacetylmannosamine-tetraacylated-ac4mannaz/ Tue, 19 Sep 2023 19:17:09 +0000 https://staging.vectorlabs.com/?post_type=product&p=21787 Description

The unnatural azido-containing monosaccharide building block. The azide moiety can be used for modification though chemoselective ligation chemistries including CuAAC, Cu-free click reaction or Staudinger ligation. The acetyl groups increase solubility in many solvents and make handling of this reagent easier.

Specifications

Unit Size 5 mg, 25 mg, 100 mg
Molecular weight 430.37
Chemical composition C16H22N4O10
CAS 361154-30-5
Solubility DMSO, DMF, DCM, THF, Chloroform
Purity >90%
Appearance Slightly grey amorphous solid
Storage Conditions -20°C.
Shipping Conditions Ambient temperature

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Description

The unnatural azido-containing monosaccharide building block. The azide moiety can be used for modification though chemoselective ligation chemistries including CuAAC, Cu-free click reaction or Staudinger ligation. The acetyl groups increase solubility in many solvents and make handling of this reagent easier.

Specifications

Unit Size5 mg, 25 mg, 100 mg
Molecular weight430.37
Chemical compositionC16H22N4O10
CAS361154-30-5
SolubilityDMSO, DMF, DCM, THF, Chloroform
Purity>90%
AppearanceSlightly grey amorphous solid
Storage Conditions-20°C.
Shipping ConditionsAmbient temperature

Documents

Selected References

  1. Ranzinger, R., et al. (2020). Mass Spectrometric Method for the Unambiguous Profiling of Cellular Dynamic Glycosylation. ACS Chem. Biol., 15, 10, 2692-2701. [ACSPublications]
  2. Loebel, C., et al. (2020). Metabolic Labeling to Probe the Spatiotemporal Accumulation of Matrix at the Chondrocyte–Hydrogel Interface. Adv. Funct. Mater. [PubMed]
  3. Song, S., et al. (2020). In Situ One-Step Fluorescence Labeling Strategy of Exosomes via Bioorthogonal Click Chemistry for Real-Time Exosome Tracking In Vitro and In Vivo. Bioconjugate Chem., 31(5), 1562-74. [PubMed]
  4. Kim, S. H., et al. (2014). Cell labeling and tracking method without distorted signals by phagocytosis of macrophages . Theranostics, 4 (4), 420-31. [PubMed]

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]]> L-Azidohomoalanine (AHA) https://staging.vectorlabs.com/products/l-azidohomoalanine-aha/ Tue, 19 Sep 2023 19:15:26 +0000 https://staging.vectorlabs.com/?post_type=product&p=21748 Description

L-Azidohomoalanine (AHA) is an amino acid analog of methionine that contains a very small modification, specifically an azido moiety that can be fed to cultured cells and incorporated into proteins during active protein synthesis. Detection utilizes the chemoselective ligation or “click ” reaction between an azide and an alkyne or cyclooctyne. The azido-modified protein is detected with either fluorescent alkyne or biotin alkyne. Detection sensitivity with these reagents in 1-D gels and Western blots is in the low femtomole range and compatible with downstream LC-MS⁄MS and MALDI MS analysis.

Specifications

Unit Size 25 mg, 100 mg, 1000 mg, 5 g
Molecular weight 180.59 (HCl salt)
Chemical composition C4H9ClN4O2 (HCl salt)
CAS 942518-29-8
Solubility Water, DMSO, DMF
Purity >95% (H NMR)
Appearance White crystalline
Storage Conditions -20°C.
Shipping Conditions Ambient temperature

 

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Description

L-Azidohomoalanine (AHA) is an amino acid analog of methionine that contains a very small modification, specifically an azido moiety that can be fed to cultured cells and incorporated into proteins during active protein synthesis. Detection utilizes the chemoselective ligation or “click ” reaction between an azide and an alkyne or cyclooctyne. The azido-modified protein is detected with either fluorescent alkyne or biotin alkyne. Detection sensitivity with these reagents in 1-D gels and Western blots is in the low femtomole range and compatible with downstream LC-MS⁄MS and MALDI MS analysis.

Specifications

Unit Size25 mg, 100 mg, 1000 mg, 5 g
Molecular weight180.59 (HCl salt)
Chemical compositionC4H9ClN4O2 (HCl salt)
CAS942518-29-8
SolubilityWater, DMSO, DMF
Purity>95% (H NMR)
AppearanceWhite crystalline
Storage Conditions-20°C.
Shipping ConditionsAmbient temperature

Documents

Selected References

  1. Loebel , C., et al. (2022). Metabolic labeling of secreted matrix to investigate cell-material interactions in tissue engineering and mechanobiology. Nat Protoc., 10.1038, Online ahead of print. [PubMed]
  2. Bruna, R. E., et al. (2021). Limitation of phosphate assimilation maintains cytoplasmic magnesium homeostasis. Proc Natl Acad Sci U S A., 118 (11), e2021370118. [PubMed]
  3. Costello, A., et al. (2021). Directed evolution of rRNA improves translation kinetics and recombinant protein yield. Nat Commun., 12 (1), 5638. [PubMed]
  4. Kwong, J. Q., et al. (2021). Mitochondrial functional resilience after TFAM ablation in the adult heart. Am J Physiol Cell Physiol., 320 (6), C929-C942. [PubMed]
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]]> PC Azido-NHS Ester https://staging.vectorlabs.com/products/pc-azido-nhs-ester/ Tue, 19 Sep 2023 17:42:49 +0000 https://staging.vectorlabs.com/?post_type=product&p=21212 Description

Photocleavable Azido-NHS ester reagent contains an amine reactive group linked to azido moiety through a spacer arm containing a photocleavable moiety. Molecules jointed through photocleavable linker can be efficiently photoreleased, typically >90% in 5-25 minutes using an inexpensive, near-UV, low intensity lamp (e.g. 365 nm lamp at 1-5 mW/cm2).

Specifications

Unit Size 10 mg, 25 mg, 100 mg
Molecular weight 640.59
Chemical composition C26H26N6O13
CAS N/A
Solubility DMSO, DMF, DCM, THF, Chloroform
Purity >95% (HPLC)
Appearance Yellow to slightly orange oil
Storage Conditions -20°C. Desiccate
Shipping Conditions Frozen

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Description

Photocleavable Azido-NHS ester reagent contains an amine reactive group linked to azido moiety through a spacer arm containing a photocleavable moiety. Molecules jointed through photocleavable linker can be efficiently photoreleased, typically >90% in 5-25 minutes using an inexpensive, near-UV, low intensity lamp (e.g. 365 nm lamp at 1-5 mW/cm2).

Specifications

Unit Size10 mg, 25 mg, 100 mg
Molecular weight640.59
Chemical compositionC26H26N6O13
CASN/A
SolubilityDMSO, DMF, DCM, THF, Chloroform
Purity>95% (HPLC)
AppearanceYellow to slightly orange oil
Storage Conditions-20°C. Desiccate
Shipping ConditionsFrozen

Documents

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