Iodoacetamide Alkyne is a broad spectrum cysteine reactive probe that reacts with nucleophiles, such as cysteine in proteins and peptides to covalently bind cysteine residues to alkyne. The alkyne group can be used as a click-chemistry handle to attach a fluorophore or biotin to reactive cysteines in proteins for visualization by in-gel fluorescence or identification by mass spectrometry, respectively.

Specifications

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F-ara-EdU is a probe for DNA synthesis that is less toxic compared to 5-ethynyl-2′-deoxyuridine (EdU) and BrdU. Ideally suited for metabolic DNA labeling experiments where long-term cell survival and/or deep-tissue imaging are desired. The alkyne handler can be used for subsequent ligation to azide-containing molecules through a highly efficient click chemistry reaction.

Specifications

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Ac4GalNAl is an unnatural alkyne-containing monosaccharide building block. The alkyne moiety can be used for modification though chemoselective ligation chemistries including CuAAC or various Pd-catalyzed coupling reactions. The acetyl groups increase solubility in many solvents and make handling of this building easier.

Specifications

The post N-(4-pentynoyl)-galactosamine tetraacylated (Ac4GalNAl) appeared first on VectorLabs.

Ac4GlcNAl is an unnatural alkyne-containing monosaccharide building block. The alkyne moiety can be used for modification though chemoselective ligation chemistries including CuAAC or various Pd-catalyzed coupling reactions. The acetyl groups increase solubility in many solvents and make handling of this reagent easier.

Specifications

The post N-(4-pentynoyl)-glucosamine tetraacylated (Ac4GlcNAl) appeared first on VectorLabs.

Ac4ManNAl is an unnatural alkyne-containing monosaccharide building block. The alkyne moiety can be used for modification though chemoselective ligation chemistries including CuAAC or various Pd-catalyzed coupling reactions. The acetyl groups increase solubility in many solvents and make handling of this reagent easier.

Specifications

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L-Homopropargylglycine (HPG) is an amino acid analog of methionine that contains a very small modification, specifically an alkyne moiety that can be fed to cultured cells and incorporated into proteins during active protein synthesis. Detection utilizes the chemoselective ligation or “click ” reaction between an azide and an alkyne or cyclooctyne. The alkyne-modified protein is detected with either fluorescent azide or biotin azide. Detection sensitivity with these reagents in 1-D gels and Western blots is in the low femtomole range and compatible with downstream LC-MS⁄MS and MALDI MS analysis.

Specifications

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Our high purity EdU (5-Ethynyl-2′-deoxyuridine) reagent is manufactured to ensure the highest possible overall product integrity, consistency and performance for the intended research applications.

EdU is a thymidine analog that can be incorporated into replicating DNA for detection of cell proliferations. The alkyne handler can be used for subsequent ligation to azide-containing molecules through a highly efficient click chemistry reaction.

Specifications

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5-Ethynyl uridine (5-EU) is an alkyne-containing metabolic labeling reagent used to detect global RNA transcription temporally and spatially in both in-vitro and in-vivo with the modified nucleotide. The alkyne handler can be used for subsequent ligation to azide-containing molecules through a highly efficient click chemistry reaction.

Molecular biology grade 5-Ethynyl Uridine is HPLC purified to achieve the highest purity possible.

Specifications

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Photocleavable Alkyne-NHS ester reagent contains an amine reactive group linked to terminal alkyne moiety through a spacer arm containing a photocleavable moiety. Molecules jointed through photocleavable linker can be efficiently photoreleased, typically >90% in 5-25 minutes using an inexpensive, near-UV, low intensity lamp (e.g. 365 nm lamp at 1-5 mW/cm²).

Specifications

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