Tetrazines demonstrate exceptionally fast cycloaddition kinetics (up to 30 000 M^-1 s^-1) with trans-cyclooctenes (TCO) as the dienophile, the fastest kinetics ever reported for any bioorthogonal reaction. In addition, inverse-Electron-Demand Diels-Alder reaction of tetrazines trans-cyclooctenea forms a stable covalent bond and does not require Cu-catalyst or elevated temperatures.

AZDye 680R is a bright and photostable near IR dye that emits fluorescence at about 700 nm in the borderline spectral region between far-red and near-IR. AZDye 680R dye is water soluble and pH-insensitive from pH 4 to pH 10. AZDye 680R is a rhodamine-based dye, and like rhodamine dyes in general, it is very bright and exceptionally photostable (Figure 1). The superior photostability and excellent brightness of AZDye 680R makes this dye an ideal for confocal microscopy, single molecule imaging and other applications that demand both brightness and photostability.

[caption id="attachment_26351" align="alignnone" width="1000"] Abs/Em Spectra[/caption]

Specifications

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A bright, red-fluorescent probe used for detection TCO-tagged biopolymers. AZDye 568 Tetrazine demonstrates exceptionally fast cycloaddition kinetics (up to 30 000 M^-1 s^-1) with trans-cyclooctenes (TCO) as the dienophile, the fastest kinetics ever reported for any bioorthogonal reaction. In applications such as in vivo cancer imaging or pre‐targeted cell labeling studies where rapid reaction kinetics is a must AZDye 568 Tetrazine probe would of great value.

Tetrazines demonstrate exceptionally fast cycloaddition kinetics (up to 30 000 M^-1 s^-1) with trans-cyclooctenes (TCO) as the dienophile, the fastest kinetics ever reported for any bioorthogonal reaction. In addition, inverse-Electron-Demand Diels-Alder reaction of tetrazines trans-cyclooctenea forms a stable covalent bond and does not require Cu-catalyst or elevated temperatures.

AZDye 568™ is a bright, and highly photostable, orange-fluorescent probe optimally excited by the 568 nm laser line on the Ar-Kr mixed-gas laser. This probe is water-soluble and its fluorescence is pH independent over a wide pH range. The brightness and photostability of this dye are best suited to direct imaging of low-abundance targets.

[caption id="attachment_25619" align="alignnone" width="1000"] Abs/Em Spectra[/caption]

Specifications

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A building block used for derivatization of carboxylic acids or activated esters with tetrazine moiety. Tetrazine Amine demonstrates exceptionally fast cycloaddition kinetics (up to 30 000 M^-1 s^-1) with trans-cyclooctene (TCO) as the dienophile, the fastest kinetics ever reported for any bioorthogonal reaction. This reagent is supplied as HCl salt for improved stability and easy handling.

Specifications

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Tetrazine Acid demonstrates exceptionally fast cycloaddition kinetics (up to 30 000 M^-1 s^-1) with trans-cyclooctene (TCO) as the dienophile, the fastest kinetics ever reported for any bioorthogonal reaction. The chemical stability tetrazines is substantially lower compared to methyltetrazines, which requires more careful selection of reagents and conditions for chemical transformation, however it is in acceptable range for many applications.

Specifications

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Tetrazine-PEG5-NHS Ester is an amine-reactive labeling reagent often used for modification of proteins, peptides, or amine-modified oligonucleotides with tetrazine moiety. Hydrogen substituted tetrazines demonstrate exceptionally fast kinetics, generally at least 10 fold faster compared to methyl substituted tetrazines. The aqueous solubility of this reagent is substantially enhanced by a hydrophilic polyethylene glycol (PEG5) spacer arm.

Specifications

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Tetrazine-NHS Ester demonstrates exceptionally fast cycloaddition kinetics (up to 30 000 M^-1 s^-1) with trans-cyclooctene (TCO) as the dienophile, the fastest kinetics ever reported for any bioorthogonal reaction. The chemical stability tetrazines is substantially lower compared to Methyltetrazines, which requires more careful selection of reagents and conditions for chemical transformation, however it is in acceptable range for many applications.

This product is not recommended for labeling of proteins or any other biopolymers in aqueous buffers due to poor aqueous solubility.

Specifications

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