TCO-NHS Ester is an amine-reactive building block used for modification of amine-containing molecule in organic media. TCO-NHS ester is supplied as a single axial (minor, rel-(1S-2S-5E-pS), ref 1 and 4) diastereomer. Specially formulated crystalline provides easy handling and extended shelf life.

Axial TCO derivatives demonstrate much higher reactivity in the inverse electron demand cycloaddition reaction compared to equatorial TCO derivatives. Up to 10-fold increase in reactivity was reported (ref 4) compared to equatorial TCO derivatives. At the same time, the axial TCO derivatives undergo cis-trans deactivation at faster rate compared to equatorial TCO derivatives (ref 4).

Specifications

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Sulfo TCO-Maleimide a water-soluble reagent that enables simple and efficient incorporation of TCO moiety onto cysteine-containing peptides or other thiol-containing molecules. The maleimide group specifically and efficiently reacts with reduced thiols (sulfhydryl groups, –SH) at pH 6.5 to 7.5 to form stable thioether bonds. The hydrophilic sulfonated spacer arm greatly improves water solubility of TCO derivatized molecules, in many cases making it completely soluble in aqueous media. A short spacer arm adds minimal mass to modified molecules

Specifications

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TCO-PEG4-NHS Ester is a pegylated lebeling reagent with enhanced solubility in aqueous buffers used for TCO labeling of antibodies, proteins and other primary amine-containing macromolecules. A long, hydrophilic PEG4 spacer arm provides a long and flexible connection that minimizes steric hindrance involved with ligation to complementary tetrazine-containing molecules.

Specifications

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A carboxyl-reactive building block with enhanced solubility in aqueous media. Often a reagent of choice for modification biopolymers, peptides, and magnetic beads through carboxyl groups in the presence of activators (e.g. EDC, or DCC) or activated esters (e.g. NHS esters). A hydrophilic PEG3 spacer arm provides a long and flexible connection that minimizes steric hindrance involved with ligation to complementary tetrazine-containing molecules.

Specifications

The post TCO-PEG3-Amine appeared first on VectorLabs.

A building block used for derivatizing carboxyl groups in the presence of activators (e.g. EDC, or DCC) or activated esters (e.g. NHS esters) with TCO moiety through a stable amide bond. A short spacer arm adds minimal mass to modified molecules (262.8 daltons). TCO-Amine is supplied as specially formulated HCl salt (crystalline) for easy handling and extended shelf life.

Specifications

The post TCO – Amine, HCl Salt appeared first on VectorLabs.

An amine-reactive, non-activated building block with enhanced solubility in aqueous media used for derivatizing amine-containing molecule in the presence of activators (e.g. EDC, or HATU) through a stable amide bond.

Specifications

The post TCO-PEG4-Acid appeared first on VectorLabs.

TCO-PEG4-TFP Ester is an amine-reactive, labeling reagent used to modify proteins, antibodies, and other amine-containing biopolymers. The hydrophilic polyethylene glycol (PEG) spacer arm imparts water solubility and provides a long and flexible connection that minimizes steric hindrance involved with ligation to complementary tetrazine-containing molecules.

The N-hydroxysuccinimide (NHS, also known as HOSu) esters of carboxylic acids are the most widely used reactive esters for modifying amine-containing peptides and proteins. NHS esters are notorious for having a short, pH-dependent half-life in aqueous media. A 2,3,5,6-tetrafluorophenol (TFP) is a different type of reactive ester that displays much better stability toward hydrolysis in aqueous media resulting in more efficiency and better reproducible labeling of biopolymers. TFP ester of carboxylic acids react with primary amines at the same rate as NHS ester forming covalent amide bond that is identical to one formed by the reaction between primary amines and NHS esters or sulfo-NHS esters.

Specifications

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TCO-PNB Ester is an amine-reactive building block used for modification of amine-containing molecule in organic media. TCO-PNB Ester is supplied as a single equatorial (major, rel-(1S-2S-5E-pR), ref 1 and 4) diastereomer. Specially formulated crystalline provides easy handling and extended shelf life.

TCO-PNB Ester is not recommended for labeling of proteins in aqueous buffers due to poor level of activation of labeled proteins (Rahim, M.K., et al., Bioconjugate Chem. 2015, 26, 352-360). TCO-PEG4-NHS Ester and TCO-PEG12-NHS Ester are well suited for protein and antibody labeling in aqueous buffers.

Specifications

The post TCO-PNB Ester (equatorial) appeared first on VectorLabs.

0TCO-PEG3-Maleimide enables simple and efficient incorporation of TCO moiety onto antibodies, cysteine-containing peptides and other thiol-containing molecules. The maleimide group specifically and efficiently reacts with reduced thiols (sulfhydryl groups, –SH) at pH 6.5 to 7.5 to form stable thioether bonds. The hydrophilic polyethylene glycol (PEG) spacer arm improves solubility in aqueous buffers.

Specifications

The post TCO-PEG3-Maleimide appeared first on VectorLabs.