SPDP – VectorLabs https://staging.vectorlabs.com From linker design and synthesis, bioconjugated design and manufacturing, biomolecule labeling and functionalization, and detection system and solutions, our team is here to help you move forward, with impact. Wed, 22 Oct 2025 17:19:32 +0000 en-US hourly 1 https://wordpress.org/?v=6.9 https://staging.vectorlabs.com/wp-content/uploads/2024/11/V-favicon-02-100x100.png SPDP – VectorLabs https://staging.vectorlabs.com 32 32 SPDP-dPEG®₃₆-NHS ester https://staging.vectorlabs.com/products/spdp-dpeg36-nhs-ester/ Sat, 24 Feb 2024 02:13:33 +0000 https://staging.vectorlabs.com/?post_type=product&p=37063 SPDP-dPEG®36-NHS ester, product number QBD-10867, is a long (115 atoms), hydrophilic, non-immunogenic, heterobifunctional crosslinker that has the same functionality as the widely popular, but unfortunately hydrophobic, SPDP crosslinker. It contains an amine-reactive N-hydroxysuccinimidyl (NHS) ester on one end of the dPEG®36 linker and a thiol-reactive SPDP (also known as OPSS) reactive group on the other end. The long spacer arm may facilitate the reaction of the SPDP end of the molecule with buried thiol groups.

Among the most popular heterobifunctional crosslinking reagents used in bioconjugate chemistry are those compounds that conjugate molecules containing free amine groups with molecules that contain sulfhydryl groups. SPDP (N-succinimidyl 3-(2-pyridyldithio)-propionate) is one of the most popular versions of this type of crosslinker. Unfortunately, SPDP and its popular varieties (LC-SPDP and Sulfo-LC-SPDP) are hydrophobic molecules. When conjugated to biomolecules, care must be taken not to modify the target molecules too much, because this can precipitate the conjugate products.

By contrast, SPDP-dPEG®36-NHS ester, product number QBD-10867, is used like SPDP and its related hydrophobic products to modify peptides, proteins, and other biomolecules. However, precautions against overly modifying the target molecule are not necessary. The water-soluble dPEG® product will not cause the resulting conjugates to precipitate due to excessive hydrophobicity.

Specifications

Unit Size 100mg, 1000mg
Molecular Weight 1969.33; single compound
Chemical formula C₈₇H₁₆₁N₃O₄₁S₂
CAS 924280-65-9
Purity > 98%
Spacers dPEG® Spacer is 115 atoms and 137.8 Å
Shipping Ambient
Typical solubility properties (for additional information contact Customer Support) Methylene chloride, Acetonitrile, DMAC or DMSO.
Storage and handling -20°C; Always let come to room temperature before opening; be careful to limit exposure to moisture and restore under an inert atmosphere; stock solutions can be prepared with dry solvent and kept for several days (freeze when not in use). dPEG® pegylation compounds are generally hygroscopic and should be treated as such. This will be less noticeable with liquids, but the solids will become tacky and difficult to manipulate, if care is not taken to minimize air exposure.

The post SPDP-dPEG®₃₆-NHS ester appeared first on VectorLabs.

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Description

SPDP-dPEG®36-NHS ester, product number QBD-10867, is a long (115 atoms), hydrophilic, non-immunogenic, heterobifunctional crosslinker that has the same functionality as the widely popular, but unfortunately hydrophobic, SPDP crosslinker. It contains an amine-reactive N-hydroxysuccinimidyl (NHS) ester on one end of the dPEG®36 linker and a thiol-reactive SPDP (also known as OPSS) reactive group on the other end. The long spacer arm may facilitate the reaction of the SPDP end of the molecule with buried thiol groups.

Among the most popular heterobifunctional crosslinking reagents used in bioconjugate chemistry are those compounds that conjugate molecules containing free amine groups with molecules that contain sulfhydryl groups. SPDP (N-succinimidyl 3-(2-pyridyldithio)-propionate) is one of the most popular versions of this type of crosslinker. Unfortunately, SPDP and its popular varieties (LC-SPDP and Sulfo-LC-SPDP) are hydrophobic molecules. When conjugated to biomolecules, care must be taken not to modify the target molecules too much, because this can precipitate the conjugate products.

By contrast, SPDP-dPEG®36-NHS ester, product number QBD-10867, is used like SPDP and its related hydrophobic products to modify peptides, proteins, and other biomolecules. However, precautions against overly modifying the target molecule are not necessary. The water-soluble dPEG® product will not cause the resulting conjugates to precipitate due to excessive hydrophobicity.

Specifications

Unit Size100mg, 1000mg
Molecular Weight1969.33; single compound
Chemical formulaC₈₇H₁₆₁N₃O₄₁S₂
CAS924280-65-9
Purity> 98%
SpacersdPEG® Spacer is 115 atoms and 137.8 Å
ShippingAmbient
Typical solubility properties (for additional information contact Customer Support)Methylene chloride, Acetonitrile, DMAC or DMSO.
Storage and handling-20°C; Always let come to room temperature before opening; be careful to limit exposure to moisture and restore under an inert atmosphere; stock solutions can be prepared with dry solvent and kept for several days (freeze when not in use). dPEG® pegylation compounds are generally hygroscopic and should be treated as such. This will be less noticeable with liquids, but the solids will become tacky and difficult to manipulate, if care is not taken to minimize air exposure.



Documents

References

Greg T. Hermanson, Bioconjugate Techniques, 2nd Edition, Elsevier Inc., Burlington, MA 01803, April, 2008 (ISBN-13: 978-0-12-370501-3; ISBN-10: 0-12-370501-0); See pp. 276-335 for general description and use of heterobifunctional crosslinkers, and the specific sample protocol for SPDP and LC-SPDP on pp. 286-288. See Greg’s extensive index on pp. 1192-1193 for references to a number and range of applications and their respective protocols.

Greg T. Hermanson, Bioconjugate Techniques, 3rd Edition, Elsevier, Waltham, MA 02451, 2013, ISBN 978-0-12-382239-0; See Chapter 18, Discrete PEG Reagents, pp. 787-821, for a full overview of the dPEG® products.

Metronomic Doses of Temozolomide Enhance the Efficacy of Carbon Nanotube CpG Immunotherapy in an Invasive Glioma Model. Mao Ouyang, Ethan E. White, Hui Ren, Qin Guo, Ian Zhang, Hang Gao, Song Yanyan, Xuebo Chen, Yiming Weng, Anna Da Fonseca, Sunny Shah, Edwin R. Manuel, Leying Zhang, Steven L. Vonderfecht, Darya Alizadeh, Jacob M. Berlin, and Behnam Badie. PLoS One. 2016, 11 (2) e0148139. February 1, 2016. DOI: 10.1371/journal.pone.0148139.

Single-wavelength-excited fluorogenic nanoprobe for accurate realtime ratiometric analysis of broad pH fluctuations in mitophagy. Xin Zhang, Juan Chen, Jiwen Hu, Anna du Rietz, Xiongyu Wu, Ruilong Zhang, Zhongping Zhang, Kajsa Uvdal, Zhangjun Hu. Springer Link. 2022. May 11, 2022. doi.org/10.1007/s12274-022-4325-3

The post SPDP-dPEG®₃₆-NHS ester appeared first on VectorLabs.

]]> SPDP-dPEG®₂₄-NHS ester https://staging.vectorlabs.com/products/spdp-dpeg24-nhs-ester/ Sat, 24 Feb 2024 02:10:19 +0000 https://staging.vectorlabs.com/?post_type=product&p=36121 SPDP-dPEG®24-NHS ester, product number QBD-10379, is a long (80 atoms), hydrophilic, non-immunogenic, heterobifunctional crosslinker that has the same functionality as the widely popular, but unfortunately hydrophobic, SPDP crosslinker. It contains an amine-reactive N-hydroxysuccinimidyl (NHS) ester on one end of the dPEG®24 linker and a thiol-reactive SPDP (also known as OPSS) reactive group on the other end. The longer spacer arm may facilitate the reaction of the SPDP end of the molecule with buried thiol groups. Moreover, unlike the traditional SPDP crosslinkers, the over-conjugation of proteins or peptides with QBD-10379 will not cause the precipitation of conjugates due to excessive hydrophobicity.

Among the most popular heterobifunctional crosslinking reagents used in bioconjugate chemistry are those compounds that conjugate molecules containing free amine groups with molecules that contain sulfhydryl groups. SPDP (N-succinimidyl 3-(2-pyridyldithio)-propionate) is one of the most popular versions of this type of crosslinker. Unfortunately, SPDP and its popular varieties (LC-SPDP and Sulfo-LC-SPDP) are hydrophobic molecules. When conjugated to biomolecules, care must be taken not to modify the target molecules too much, because this can precipitate the conjugate products.

By contrast, SPDP-dPEG®24-NHS ester, product number QBD-10379, is used like SPDP and its related hydrophobic products. However, precautions against overly modifying the target molecule are not necessary. The water-soluble dPEG® product will not cause the resulting conjugates to precipitate due to excessive hydrophobicity.

Specifications

Unit Size 100 mg, 1000 mg
Molecular Weight 1440.70; single compound
Chemical formula C₆₃H₁₁₃N₃O₂₉S₂
CAS 924280-65-9
Purity > 97%
Spacers dPEG® Spacer is 80 atoms and 97.3 Å
Shipping Ambient
Typical solubility properties (for additional information contact Customer Support) Methylene chloride, Acetonitrile, DMAC or DMSO.
Storage and handling -20°C; Always let come to room temperature before opening; be careful to limit exposure to moisture and restore under an inert atmosphere; stock solutions can be prepared with dry solvent and kept for several days (freeze when not in use). dPEG® pegylation compounds are generally hygroscopic and should be treated as such. This will be less noticeable with liquids, but the solids will become tacky and difficult to manipulate, if care is not taken to minimize air exposure.

The post SPDP-dPEG®₂₄-NHS ester appeared first on VectorLabs.

]]>

Description

SPDP-dPEG®24-NHS ester, product number QBD-10379, is a long (80 atoms), hydrophilic, non-immunogenic, heterobifunctional crosslinker that has the same functionality as the widely popular, but unfortunately hydrophobic, SPDP crosslinker. It contains an amine-reactive N-hydroxysuccinimidyl (NHS) ester on one end of the dPEG®24 linker and a thiol-reactive SPDP (also known as OPSS) reactive group on the other end. The longer spacer arm may facilitate the reaction of the SPDP end of the molecule with buried thiol groups. Moreover, unlike the traditional SPDP crosslinkers, the over-conjugation of proteins or peptides with QBD-10379 will not cause the precipitation of conjugates due to excessive hydrophobicity.

Among the most popular heterobifunctional crosslinking reagents used in bioconjugate chemistry are those compounds that conjugate molecules containing free amine groups with molecules that contain sulfhydryl groups. SPDP (N-succinimidyl 3-(2-pyridyldithio)-propionate) is one of the most popular versions of this type of crosslinker. Unfortunately, SPDP and its popular varieties (LC-SPDP and Sulfo-LC-SPDP) are hydrophobic molecules. When conjugated to biomolecules, care must be taken not to modify the target molecules too much, because this can precipitate the conjugate products.

By contrast, SPDP-dPEG®24-NHS ester, product number QBD-10379, is used like SPDP and its related hydrophobic products. However, precautions against overly modifying the target molecule are not necessary. The water-soluble dPEG® product will not cause the resulting conjugates to precipitate due to excessive hydrophobicity.

Specifications

Unit Size100 mg, 1000 mg
Molecular Weight1440.70; single compound
Chemical formulaC₆₃H₁₁₃N₃O₂₉S₂
CAS924280-65-9
Purity> 97%
SpacersdPEG® Spacer is 80 atoms and 97.3 Å
ShippingAmbient
Typical solubility properties (for additional information contact Customer Support)Methylene chloride, Acetonitrile, DMAC or DMSO.
Storage and handling-20°C; Always let come to room temperature before opening; be careful to limit exposure to moisture and restore under an inert atmosphere; stock solutions can be prepared with dry solvent and kept for several days (freeze when not in use). dPEG® pegylation compounds are generally hygroscopic and should be treated as such. This will be less noticeable with liquids, but the solids will become tacky and difficult to manipulate, if care is not taken to minimize air exposure.

Documents

References

Greg T. Hermanson, Bioconjugate Techniques, 2nd Edition, Elsevier Inc., Burlington, MA 01803, April, 2008 (ISBN-13: 978-0-12-370501-3; ISBN-10: 0-12-370501-0); See pp. 276-335 for general description and use of heterobifunctional crosslinkers, and the specific sample protocol for SPDP and LC-SPDP on pp. 286-288. See Greg’s extensive index on pp. 1192-1193 for references to a number and range of applications and their respective protocols.

Greg T. Hermanson, Bioconjugate Techniques, 3rd Edition, Elsevier, Waltham, MA 02451, 2013, ISBN 978-0-12-382239-0; See Chapter 18, Discrete PEG Reagents, pp. 787-821, for a full overview of the dPEG® products.

Different pH-Dependencies of the Two Synaptic Adhesion Molecules N-Cadherin and Cadherin-11 and the Possible Functional Implication for Long-term Potentiation. WERNER BAUMGARTNER, ARMIN OSMANAGIC, MARITA GEBHARD,SANDRA KRAEMER, AND NIKOLA GOLENHOFEN. SYNAPSE. 2013, 67 (10) pp 705-715. June 3, 2013. DOI: 10.1002/syn.21679.

Systemically administered collagen-targeted gold nanoparticles bind to arterial injury following vascular interventions. Molly Wasserman Meyers, Jonathan S Rink, Qun Jiang, Megan E Kelly, Janet M Vercammen, Colby S Thaxton, and Melina R Kibbe. Physiological Reports. 2016, 5 (4): e13128. December 17, 2016. DOI: 10.14814/phy2.13128.

The post SPDP-dPEG®₂₄-NHS ester appeared first on VectorLabs.

]]> SPDP-dPEG®₁₂-NHS ester https://staging.vectorlabs.com/products/spdp-dpeg12-nhs-ester/ Sat, 24 Feb 2024 02:10:18 +0000 https://staging.vectorlabs.com/?post_type=product&p=36101 SPDP-dPEG®12-NHS ester, product number QBD-10378, is a medium-length (44 atoms), hydrophilic, non-immunogenic, heterobifunctional crosslinker that has the same functionality as the widely popular, but unfortunately hydrophobic, SPDP crosslinker. It contains an amine-reactive N-hydroxysuccinimidyl (NHS) ester on one end of the dPEG®12 linker and a thiol-reactive SPDP (also known as OPSS) reactive group on the other end. The longer spacer arm may facilitate the reaction of the SPDP end of the molecule with buried thiol groups.

Among the most popular heterobifunctional crosslinking reagents used in bioconjugate chemistry are those compounds that conjugate molecules containing free amine groups with molecules that contain sulfhydryl groups. SPDP (N-succinimidyl 3-(2-pyridyldithio)-propionate) is one of the most popular versions of this type of crosslinker. Unfortunately, SPDP and its popular varieties (LC-SPDP and Sulfo-LC-SPDP) are hydrophobic molecules. When conjugated to biomolecules, care must be taken not to modify the target molecules too much, because this can precipitate the conjugate products.

By contrast, SPDP-dPEG®12-NHS ester, product number QBD-10378, is used like SPDP and its related hydrophobic products. However, precautions against overly modifying the target molecule are not necessary. The water-soluble dPEG® product will not cause the resulting conjugates to precipitate due to excessive hydrophobicity.

Specifications

Unit Size 100 mg, 1000 mg
Molecular Weight 912.08; single compound
Chemical formula C₂₉H₆₅N₃O₁₇S₂
CAS 924280-65-9
Purity > 97%
Spacers dPEG® Spacer is 44 atoms and 51.3 Å
Shipping Ambient
Typical solubility properties (for additional information contact Customer Support) Methylene chloride, Acetonitrile, DMAC or DMSO.
Storage and handling -20°C; Always let come to room temperature before opening; be careful to limit exposure to moisture and restore under an inert atmosphere; stock solutions can be prepared with dry solvent and kept for several days (freeze when not in use). dPEG® pegylation compounds are generally hygroscopic and should be treated as such. This will be less noticeable with liquids, but the solids will become tacky and difficult to manipulate, if care is not taken to minimize air exposure.

The post SPDP-dPEG®₁₂-NHS ester appeared first on VectorLabs.

]]>

Description

SPDP-dPEG®12-NHS ester, product number QBD-10378, is a medium-length (44 atoms), hydrophilic, non-immunogenic, heterobifunctional crosslinker that has the same functionality as the widely popular, but unfortunately hydrophobic, SPDP crosslinker. It contains an amine-reactive N-hydroxysuccinimidyl (NHS) ester on one end of the dPEG®12 linker and a thiol-reactive SPDP (also known as OPSS) reactive group on the other end. The longer spacer arm may facilitate the reaction of the SPDP end of the molecule with buried thiol groups.

Among the most popular heterobifunctional crosslinking reagents used in bioconjugate chemistry are those compounds that conjugate molecules containing free amine groups with molecules that contain sulfhydryl groups. SPDP (N-succinimidyl 3-(2-pyridyldithio)-propionate) is one of the most popular versions of this type of crosslinker. Unfortunately, SPDP and its popular varieties (LC-SPDP and Sulfo-LC-SPDP) are hydrophobic molecules. When conjugated to biomolecules, care must be taken not to modify the target molecules too much, because this can precipitate the conjugate products.

By contrast, SPDP-dPEG®12-NHS ester, product number QBD-10378, is used like SPDP and its related hydrophobic products. However, precautions against overly modifying the target molecule are not necessary. The water-soluble dPEG® product will not cause the resulting conjugates to precipitate due to excessive hydrophobicity.

Specifications

Unit Size100 mg, 1000 mg
Molecular Weight912.08; single compound
Chemical formulaC₂₉H₆₅N₃O₁₇S₂
CAS924280-65-9
Purity> 97%
SpacersdPEG® Spacer is 44 atoms and 51.3 Å
ShippingAmbient
Typical solubility properties (for additional information contact Customer Support)Methylene chloride, Acetonitrile, DMAC or DMSO.
Storage and handling-20°C; Always let come to room temperature before opening; be careful to limit exposure to moisture and restore under an inert atmosphere; stock solutions can be prepared with dry solvent and kept for several days (freeze when not in use). dPEG® pegylation compounds are generally hygroscopic and should be treated as such. This will be less noticeable with liquids, but the solids will become tacky and difficult to manipulate, if care is not taken to minimize air exposure.



Documents

References

Greg T. Hermanson, Bioconjugate Techniques, 2nd Edition, Elsevier Inc., Burlington, MA 01803, April, 2008 (ISBN-13: 978-0-12-370501-3; ISBN-10: 0-12-370501-0); See pp. 276-335 for general description and use of heterobifunctional crosslinkers, and the specific sample protocol for SPDP and LC-SPDP on pp. 286-288. See Greg’s extensive index on pp. 1192-1193 for references to a number and range of applications and their respective protocols.

Greg T. Hermanson, Bioconjugate Techniques, 3rd Edition, Elsevier, Waltham, MA 02451, 2013, ISBN 978-0-12-382239-0; See Chapter 18, Discrete PEG Reagents, pp. 787-821, for a full overview of the dPEG® products.

Ellman’s and Aldrithiol Assay as Versatile and Complementary Tools for the Quantification of Thiol Groups and Ligands on Nanomaterials. Marko Moser, Ralf Schneider, Thomas Behnke, Thomas Schneider, Jana Falkenhagen, and Ute Resch-Genger. Analytical Chemistry. 2016, pp 1-9. July 2, 2016. DOI: 10.1021/acs.analchem.6b01798.

Refined purification strategy for reliable proteomic profiling of HDL 2/3: Impact on proteomic complexity. Michael Holzer, Sabine Kern, Ruth Birner-Grunberger, Sanja Curcic, Akos Heinemann, and Gunther Marsche. Scientific Reports. 2016, 6 (38533) pp 1-10. December 5, 2016. DOI: 10.1038/srep38533.

The post SPDP-dPEG®₁₂-NHS ester appeared first on VectorLabs.

]]> SPDP-dPEG®₈-NHS ester https://staging.vectorlabs.com/products/spdp-dpeg8-nhs-ester/ Sat, 24 Feb 2024 02:10:17 +0000 https://staging.vectorlabs.com/?post_type=product&p=36093 SPDP-dPEG®8-NHS ester, product number QBD-10376, is a medium-length (32 atoms), hydrophilic, non-immunogenic, heterobifunctional crosslinker that has the same functionality as the widely popular, but unfortunately hydrophobic, SPDP crosslinker. It contains an amine-reactive N-hydroxysuccinimidyl (NHS) ester on one end of the dPEG®8 linker, and a thiol-reactive SPDP (also known as OPSS) reactive group on the other end.

Among the most popular heterobifunctional crosslinking reagents used in bioconjugate chemistry are those compounds that conjugate molecules containing free amine groups with molecules that contain sulfhydryl groups. SPDP (N-succinimidyl 3-(2-pyridyldithio)-propionate) is one of the most popular versions of this type of crosslinker. Unfortunately, SPDP and its popular varieties (LC-SPDP and Sulfo-LC-SPDP) are hydrophobic molecules. When conjugated to biomolecules, care must be taken not to modify the target molecules too much, because this can precipitate the conjugate products.

By contrast, SPDP-dPEG®8-NHS ester, product number QBD-10376, is used like SPDP and its related hydrophobic products. However, precautions against overly modifying the target molecule are not necessary. The water-soluble dPEG® product will not cause the resulting conjugates to precipitate due to excessive hydrophobicity.

Specifications

Unit Size 100 mg, 1000 mg
Molecular Weight 735.87; single compound
Chemical formula C₃₁H₄₉N₃O₁₃S₂
CAS 1252257-56-9
Purity > 97%
Spacers dPEG® Sp
Typical solubility properties (for additional information contact Customer Support) Methylene chloride, Acetontrile, DMAC, DMSO or water.
Storage and handling -20°C; Always let come to room temperature before opening; be careful to limit exposure to moisture and restore under an inert atmosphere; stock solutions can be prepared with dry solvent and kept for several days (freeze when not in use). dPEG® pegylation compounds are generally hygroscopic and should be treated as such. This will be less noticeable with liquids, but the solids will become tacky and difficult to manipulate, if care is not taken to minimize air exposure.

The post SPDP-dPEG®₈-NHS ester appeared first on VectorLabs.

]]>

Description

SPDP-dPEG®8-NHS ester, product number QBD-10376, is a medium-length (32 atoms), hydrophilic, non-immunogenic, heterobifunctional crosslinker that has the same functionality as the widely popular, but unfortunately hydrophobic, SPDP crosslinker. It contains an amine-reactive N-hydroxysuccinimidyl (NHS) ester on one end of the dPEG®8 linker, and a thiol-reactive SPDP (also known as OPSS) reactive group on the other end.

Among the most popular heterobifunctional crosslinking reagents used in bioconjugate chemistry are those compounds that conjugate molecules containing free amine groups with molecules that contain sulfhydryl groups. SPDP (N-succinimidyl 3-(2-pyridyldithio)-propionate) is one of the most popular versions of this type of crosslinker. Unfortunately, SPDP and its popular varieties (LC-SPDP and Sulfo-LC-SPDP) are hydrophobic molecules. When conjugated to biomolecules, care must be taken not to modify the target molecules too much, because this can precipitate the conjugate products.

By contrast, SPDP-dPEG®8-NHS ester, product number QBD-10376, is used like SPDP and its related hydrophobic products. However, precautions against overly modifying the target molecule are not necessary. The water-soluble dPEG® product will not cause the resulting conjugates to precipitate due to excessive hydrophobicity.

Specifications

Unit Size100 mg, 1000 mg
Molecular Weight735.87; single compound
Chemical formulaC₃₁H₄₉N₃O₁₃S₂
CAS1252257-56-9
Purity> 97%
SpacersdPEG® Sp
Typical solubility properties (for additional information contact Customer Support)Methylene chloride, Acetontrile, DMAC, DMSO or water.
Storage and handling-20°C; Always let come to room temperature before opening; be careful to limit exposure to moisture and restore under an inert atmosphere; stock solutions can be prepared with dry solvent and kept for several days (freeze when not in use). dPEG® pegylation compounds are generally hygroscopic and should be treated as such. This will be less noticeable with liquids, but the solids will become tacky and difficult to manipulate, if care is not taken to minimize air exposure.



Documents

References

Greg T. Hermanson, Bioconjugate Techniques, 2nd Edition, Elsevier Inc., Burlington, MA 01803, April, 2008 (ISBN-13: 978-0-12-370501-3; ISBN-10: 0-12-370501-0); See pp. 276-335 for general description and use of heterobifunctional crosslinkers, and the specific sample protocol for SPDP and LC-SPDP on pp. 286-288. See Greg’s extensive index on pp. 1192-1193 for references to a number and range of applications and their respective protocols.

Greg T. Hermanson, Bioconjugate Techniques, 3rd Edition, Elsevier, Waltham, MA 02451, 2013, ISBN 978-0-12-382239-0; See Chapter 18, Discrete PEG Reagents, pp. 787-821, for a full overview of the dPEG® products.

Cellular Delivery and Antisense Effects of Peptide Nucleic Acid Conjugated to Polyethyleneimine via Disulfide Linkers. Peter R. Berthold, Takehiko Shiraishi, and Peter E. Nielsen.Bioconjugate Chem., 2010, 21 (10), pp 1933–1938 September 27, 2010. DOI: 10.1021/bc1003586.

Cell number and transfection volume dependent peptide nucleic acid antisense activity by cationic delivery methods. Laia Llovera, Peter R. Berthold, Peter E. Nielsen and Takehiko Shiraishi. Artificial DNA, PNA & XNA 2012, 3 (1) pp 22-27 March 5, 2012. DOI: 10.4161/adna.19906.

In Situ Detection of Protein Complexes and Modifications by Chemical Ligation Proximity Assay. Rui Hong, Esteban Roberts, and Christopher Bieniarz. Bioconjugate Chemistry. 2016, June 1, 2016. DOI: 10.1021/acs.bioconjchem.6b00230.

The post SPDP-dPEG®₈-NHS ester appeared first on VectorLabs.

]]> SPDP-dPEG®₄-NHS ester https://staging.vectorlabs.com/products/spdp-dpeg4-nhs-ester/ Sat, 24 Feb 2024 02:10:16 +0000 https://staging.vectorlabs.com/?post_type=product&p=36085 SPDP-dPEG®4-NHS ester, product number QBD-10374, is Vector Laboratories' water-soluble version of the immensely popular, but unfortunately hydrophobic, SPDP crosslinker. SPDP-dPEG®4-NHS ester is a heterobifunctional crosslinker. The N-hydroxysuccinimidyl (NHS) ester end of the molecule reacts with free amines. The opposite end is a 3-(2-pyridyldithio)-propionate functional group that reacts with sulfhydryl groups in thiol-containing molecules to form disulfide bonds. Between the two functional groups is a short (20 atoms), amphiphilic, single molecular weight, discrete polyethylene glycol (dPEG®) spacer.

Among the most popular heterobifunctional crosslinking reagents used in bioconjugate chemistry are those compounds that conjugate molecules containing free amine groups with molecules that contain sulfhydryl groups. SPDP (N-succinimidyl 3-(2-pyridyldithio)-propionate) is one of the most popular versions of this type of crosslinker. Unfortunately, SPDP and its popular varieties (LC-SPDP and Sulfo-LC-SPDP) are hydrophobic molecules. When conjugated to biomolecules, care must be taken not to modify the target molecules too much, because this can precipitate the conjugate products.

By contrast, SPDP-dPEG®4-NHS ester, product number QBD-10374, is used like SPDP and its related hydrophobic products. However, precautions against overly modifying the target molecule are not necessary. The water-soluble dPEG® product will not cause the resulting conjugates to precipitate due to excessive hydrophobicity.

Specifications

Unit Size 100 mg, 1000 mg
Molecular Weight 559.65; single compound
Chemical formula C₂₃H₃₃N₃O₉S₂
CAS 1334177-95-5
Purity > 97%
Spacers dPEG® Spacer is 20 atoms and 23.1 Å
Shipping Ambient
Typical solubility properties (for additional information contact Customer Support) Methylene chloride, Acetonitrile, DMAC or DMSO.
Storage and handling -20°C; Always let come to room temperature before opening; be careful to limit exposure to moisture and restore under an inert atmosphere; stock solutions can be prepared with dry solvent and kept for several days (freeze when not in use). dPEG® pegylation compounds are generally hygroscopic and should be treated as such. This will be less noticeable with liquids, but the solids will become tacky and difficult to manipulate, if care is not taken to minimize air exposure.

The post SPDP-dPEG®₄-NHS ester appeared first on VectorLabs.

]]>

Description

SPDP-dPEG®4-NHS ester, product number QBD-10374, is Vector Laboratories’ water-soluble version of the immensely popular, but unfortunately hydrophobic, SPDP crosslinker. SPDP-dPEG®4-NHS ester is a heterobifunctional crosslinker. The N-hydroxysuccinimidyl (NHS) ester end of the molecule reacts with free amines. The opposite end is a 3-(2-pyridyldithio)-propionate functional group that reacts with sulfhydryl groups in thiol-containing molecules to form disulfide bonds. Between the two functional groups is a short (20 atoms), amphiphilic, single molecular weight, discrete polyethylene glycol (dPEG®) spacer.

Among the most popular heterobifunctional crosslinking reagents used in bioconjugate chemistry are those compounds that conjugate molecules containing free amine groups with molecules that contain sulfhydryl groups. SPDP (N-succinimidyl 3-(2-pyridyldithio)-propionate) is one of the most popular versions of this type of crosslinker. Unfortunately, SPDP and its popular varieties (LC-SPDP and Sulfo-LC-SPDP) are hydrophobic molecules. When conjugated to biomolecules, care must be taken not to modify the target molecules too much, because this can precipitate the conjugate products.

By contrast, SPDP-dPEG®4-NHS ester, product number QBD-10374, is used like SPDP and its related hydrophobic products. However, precautions against overly modifying the target molecule are not necessary. The water-soluble dPEG® product will not cause the resulting conjugates to precipitate due to excessive hydrophobicity.

Specifications

Unit Size100 mg, 1000 mg
Molecular Weight559.65; single compound
Chemical formulaC₂₃H₃₃N₃O₉S₂
CAS1334177-95-5
Purity> 97%
SpacersdPEG® Spacer is 20 atoms and 23.1 Å
ShippingAmbient
Typical solubility properties (for additional information contact Customer Support)Methylene chloride, Acetonitrile, DMAC or DMSO.
Storage and handling-20°C; Always let come to room temperature before opening; be careful to limit exposure to moisture and restore under an inert atmosphere; stock solutions can be prepared with dry solvent and kept for several days (freeze when not in use). dPEG® pegylation compounds are generally hygroscopic and should be treated as such. This will be less noticeable with liquids, but the solids will become tacky and difficult to manipulate, if care is not taken to minimize air exposure.



Documents

References

Greg T. Hermanson, Bioconjugate Techniques, 2nd Edition, Elsevier Inc., Burlington, MA 01803, April, 2008 (ISBN-13: 978-0-12-370501-3; ISBN-10: 0-12-370501-0); See pp. 276-335 for general description and use of heterobifunctional crosslinkers, and the specific sample protocol for SPDP and LC-SPDP on pp. 286-288. See Greg’s extensive index on pp. 1192-1193 for references to a number and range of applications and their respective protocols.

Greg T. Hermanson, Bioconjugate Techniques, 3rd Edition, Elsevier, Waltham, MA 02451, 2013, ISBN 978-0-12-382239-0; See Chapter 18, Discrete PEG Reagents, pp. 787-821, for a full overview of the dPEG® products.

Post-transcriptional regulation of the GASC1 oncogene with active tumor-targeted siRNA-nanoparticle. Sara Movassaghian, Claudia Hildebrandt, Yuran Xie, Rayna Rosati, Ying Li, Na Hyung Kim, Denise Conti, Sandro RP da Rocha, Zeng-Quan Yang, and Olivia M. Merkel. Molecular Pharmaceutics. 2016, May 25, 2016. DOI: 10.1021/acs.molpharmaceut.5b00948.

Targeted Delivery of siRNA to Transferrin Receptor Overexpressing Tumor Cells via Peptide Modified Polyethylenimine. Yuran Xie, Bryan Killinger, Anna Moszczynska, and Olivia M. Merkel. Molecules. 2016, 21 (1334) pp 1-16. October 10, 2016. DOI: 10.3390/molecules21101334.

BSA Hydrogel Beads Functionalized with a Specific Aptamer Library for Capturing Pseudomonas aeruginosa in Serum and Blood. Markus Krämer, Ann-Kathrin Kissmann, Heinz Fabian Raber, Hu Xing, Patrizia Favella, Ingrid Müller, Barbara Spellerberg, Tanja Weil, Dennis Kubiczek, Susanne Sihler, Ulrich Ziener, and Frank Rosenau. International Journal of Molecular Sciences. 2021. Volume 22, Issue 20. October 15, 2021. https://doi.org/10.3390/ijms222011118

Highly Stable Polymer Coating on Silver Nanoparticles for Efficient Plasmonic Enhancement of Fluorescence. Ryo Kato, Mitsuhiro Uesugi, Yoshie Komatsu, Fusatoshi Okamoto, Takuo Tanaka, Fumihisa Kitawaki, and Taka-aki Tano. ACS Omega. 2022. January 7, 2022. https://doi.org/10.1021/acsomega.1c06010

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