Tyramide – VectorLabs https://staging.vectorlabs.com From linker design and synthesis, bioconjugated design and manufacturing, biomolecule labeling and functionalization, and detection system and solutions, our team is here to help you move forward, with impact. Wed, 22 Oct 2025 17:09:17 +0000 en-US hourly 1 https://wordpress.org/?v=6.9 https://staging.vectorlabs.com/wp-content/uploads/2024/11/V-favicon-02-100x100.png Tyramide – VectorLabs https://staging.vectorlabs.com 32 32 Cy3 Tyramide https://staging.vectorlabs.com/products/cy3-tyramide/ Wed, 27 Sep 2023 06:58:03 +0000 https://staging.vectorlabs.com/?post_type=product&p=27301 Description

Orange fluorescent reagent widely used for tyramide signal amplification (TSA) in IHC, ICC, FISH and multicolor FISH. Tyramide signal amplification (TSA) has proven to be a particularly versatile and powerful enzyme amplification technique with improved assay sensitivity. TSA is based on the ability of HRP, in the presence of low concentrations of hydrogen peroxide, to convert labeled tyramine-containing substrate into an oxidized, highly reactive free radical that can covalently bind to tyrosine residues at or near the HRP.

Cy3 Tyramide is a component of many tyramide signal amplification kits. This reagent can be used with any antibody conjugated to HRP for tyramide signal amplification for detection of low abundance targets in multiplexable fluorescent immunocytochemistry (ICC), immunohistochemistry (IHC ), and in situ hybridization (ISH).

[caption id="attachment_25595" align="alignnone" width="1000"] Abs/Em Spectra[/caption]

Specifications

Unit Size 150 slides, 1500 slides
Abs/Em Maxima 555/572 nm
Extinction Coefficient
155,000
Flow Cytometry Laser Line
532 nm or 555 nm
Microscopy Laser Line
532 nm or 555 nm
Spectrally Similar Dyes Alexa Fluor® 555, CF® 555, DyLight® 549, Cy3 Dye
Molecular weight 735.91 (protonated)
CAS N/A
Solubility Water, DMSO, DMF
Appearance Red solid
Storage Conditions -20°C. Desiccate
Shipping Conditions Ambient temperature

The post Cy3 Tyramide appeared first on VectorLabs.

]]>

Description

Orange fluorescent reagent widely used for tyramide signal amplification (TSA) in IHC, ICC, FISH and multicolor FISH. Tyramide signal amplification (TSA) has proven to be a particularly versatile and powerful enzyme amplification technique with improved assay sensitivity. TSA is based on the ability of HRP, in the presence of low concentrations of hydrogen peroxide, to convert labeled tyramine-containing substrate into an oxidized, highly reactive free radical that can covalently bind to tyrosine residues at or near the HRP.

Cy3 Tyramide is a component of many tyramide signal amplification kits. This reagent can be used with any antibody conjugated to HRP for tyramide signal amplification for detection of low abundance targets in multiplexable fluorescent immunocytochemistry (ICC), immunohistochemistry (IHC ), and in situ hybridization (ISH).

Specifications

Unit Size150 slides, 1500 slides
Abs/Em Maxima555/572 nm
Extinction Coefficient
155,000
Flow Cytometry Laser Line
532 nm or 555 nm
Microscopy Laser Line
532 nm or 555 nm
Spectrally Similar DyesAlexa Fluor® 555, CF® 555, DyLight® 549, Cy3 Dye
Molecular weight735.91 (protonated)
CASN/A
SolubilityWater, DMSO, DMF
AppearanceRed solid
Storage Conditions-20°C. Desiccate
Shipping ConditionsAmbient temperature

Abs/Em Spectra

AF555

Documents

FAQs

150 slides has 0.24 µmol (depending on molecular weight this is ~200 µg)
1500 slides has 2.4 µmol (depending on molecular weight this is ~2 mg)

Here is a general protocol for the 150 slide unit size. To prepare 100x stock solution, we recommend reconstituting with 150 ul of DMSO. Storage once in solution: 100X stock solution in DMSO can be stored for 6 months at 2-8 °C protected from light. Avoid moisture.
Working solutions can be made fresh by adding 1 part 100X stock solution, 1 part 0.15% H202, and 100 part buffer.
For example, you can add 5 µL of 100X Tyramide stock solution and 5 µL 0.15% H2O2 to 500 µL of buffer. The final concentration of H2O2 is 0.0015%.
Typical reaction time following the HRP reagent is 2-10 minutes but should be optimized for each workflow. Following the TSA working solution incubation, wash thoroughly.

The post Cy3 Tyramide appeared first on VectorLabs.

]]> Cy5 Tyramide https://staging.vectorlabs.com/products/cy5-tyramide/ Tue, 19 Sep 2023 20:11:03 +0000 https://staging.vectorlabs.com/?post_type=product&p=22526 Description

Far-red fluorescent reagent widely used for tyramide signal amplification (TSA) in IHC, ICC, FISH and multicolor FISH. Tyramide signal amplification (TSA) has proven to be a particularly versatile and powerful enzyme amplification technique with improved assay sensitivity. TSA is based on the ability of HRP, in the presence of low concentrations of hydrogen peroxide, to convert labeled tyramine-containing substrate into an oxidized, highly reactive free radical that can covalently bind to tyrosine residues at or near the HRP.

Cy5 Tyramide is a component of many tyramide signal amplification kits. This reagent can be used with any antibody conjugated to HRP for tyramide signal amplification for detection of low abundance targets in multiplexable fluorescent immunocytochemistry (ICC), immunohistochemistry (IHC ), and in situ hybridization (ISH).

[caption id="attachment_25905" align="alignnone" width="1000"] Abs/Em Spectra[/caption]

Specifications

Unit Size 150 slides, 1500 slides
Abs/Em Maxima 648/671 nm
Extinction Coefficient
270,000
Flow Cytometry Laser Line
633 nm or 647 nm
Microscopy Laser Line
633 nm or 647 nm
Spectrally Similar Dyes Alexa Fluor® 647, CF® 647, DyLight® 649
Molecular weight 761.28 (protonated)
CAS N/A
Solubility Water, DMSO, DMF
Appearance Blue solid
Storage Conditions -20°C. Desiccate
Shipping Conditions Ambient temperature

The post Cy5 Tyramide appeared first on VectorLabs.

]]>

Description

Far-red fluorescent reagent widely used for tyramide signal amplification (TSA) in IHC, ICC, FISH and multicolor FISH. Tyramide signal amplification (TSA) has proven to be a particularly versatile and powerful enzyme amplification technique with improved assay sensitivity. TSA is based on the ability of HRP, in the presence of low concentrations of hydrogen peroxide, to convert labeled tyramine-containing substrate into an oxidized, highly reactive free radical that can covalently bind to tyrosine residues at or near the HRP.

Cy5 Tyramide is a component of many tyramide signal amplification kits. This reagent can be used with any antibody conjugated to HRP for tyramide signal amplification for detection of low abundance targets in multiplexable fluorescent immunocytochemistry (ICC), immunohistochemistry (IHC ), and in situ hybridization (ISH).

Specifications

Unit Size150 slides, 1500 slides
Abs/Em Maxima648/671 nm
Extinction Coefficient
270,000
Flow Cytometry Laser Line
633 nm or 647 nm
Microscopy Laser Line
633 nm or 647 nm
Spectrally Similar DyesAlexa Fluor® 647, CF® 647, DyLight® 649
Molecular weight761.28 (protonated)
CASN/A
SolubilityWater, DMSO, DMF
AppearanceBlue solid
Storage Conditions-20°C. Desiccate
Shipping ConditionsAmbient temperature

Abs/Em Spectra

AF647

Documents

FAQs

150 slides has 0.24 µmol (depending on molecular weight this is ~200 µg)
1500 slides has 2.4 µmol (depending on molecular weight this is ~2 mg)

Here is a general protocol for the 150 slide unit size. To prepare 100x stock solution, we recommend reconstituting with 150 ul of DMSO. Storage once in solution: 100X stock solution in DMSO can be stored for 6 months at 2-8 °C protected from light. Avoid moisture.
Working solutions can be made fresh by adding 1 part 100X stock solution, 1 part 0.15% H202, and 100 part buffer.
For example, you can add 5 µL of 100X Tyramide stock solution and 5 µL 0.15% H2O2 to 500 µL of buffer. The final concentration of H2O2 is 0.0015%.
Typical reaction time following the HRP reagent is 2-10 minutes but should be optimized for each workflow. Following the TSA working solution incubation, wash thoroughly.

The post Cy5 Tyramide appeared first on VectorLabs.

]]> AZDye 680 Tyramide https://staging.vectorlabs.com/products/azdye-680-tyramide/ Tue, 19 Sep 2023 20:10:39 +0000 https://staging.vectorlabs.com/?post_type=product&p=22518 Description

NIR fluorescent reagent widely used for tyramide signal amplification (TSA) in IHC, ICC, FISH and multicolor FISH. Tyramide signal amplification (TSA) has proven to be a particularly versatile and powerful enzyme amplification technique with improved assay sensitivity. TSA is based on the ability of HRP, in the presence of low concentrations of hydrogen peroxide, to convert labeled tyramine-containing substrate into an oxidized, highly reactive free radical that can covalently bind to tyrosine residues at or near the HRP.

AZDye™ 680 Tyramide is a component of many tyramide signal amplification kits, including SuperBoost®. This reagent can be used with any antibody conjugated to HRP for tyramide signal amplification for detection of low abundance targets in multiplexable fluorescent immunocytochemistry (ICC), immunohistochemistry (IHC ), and in situ hybridization (ISH).

[caption id="attachment_24263" align="alignnone" width="1940"] Abs/Em Spectra[/caption]

Specifications

Unit Size 150 slides, 1500 slides
Abs/Em Maxima 678/701 nm
Extinction Coefficient
185,000
Flow Cytometry Laser Line
633 nm or 647 nm
Microscopy Laser Line
633 nm or 647 nm
Spectrally Similar Dyes Alexa Fluor® 680, IRDye® 680RD, CF™ 680 Dye
Molecular weight 947.57 (protonated)
CAS N/A
Solubility Water, DMSO, DMF
Appearance Blue solid
Storage Conditions -20°C. Desiccate
Shipping Conditions Ambient temperature

The post AZDye 680 Tyramide appeared first on VectorLabs.

]]>

Description

NIR fluorescent reagent widely used for tyramide signal amplification (TSA) in IHC, ICC, FISH and multicolor FISH. Tyramide signal amplification (TSA) has proven to be a particularly versatile and powerful enzyme amplification technique with improved assay sensitivity. TSA is based on the ability of HRP, in the presence of low concentrations of hydrogen peroxide, to convert labeled tyramine-containing substrate into an oxidized, highly reactive free radical that can covalently bind to tyrosine residues at or near the HRP.

AZDye™ 680 Tyramide is a component of many tyramide signal amplification kits, including SuperBoost®. This reagent can be used with any antibody conjugated to HRP for tyramide signal amplification for detection of low abundance targets in multiplexable fluorescent immunocytochemistry (ICC), immunohistochemistry (IHC ), and in situ hybridization (ISH).

Specifications

Unit Size150 slides, 1500 slides
Abs/Em Maxima678/701 nm
Extinction Coefficient
185,000
Flow Cytometry Laser Line
633 nm or 647 nm
Microscopy Laser Line
633 nm or 647 nm
Spectrally Similar DyesAlexa Fluor® 680, IRDye® 680RD, CF™ 680 Dye
Molecular weight947.57 (protonated)
CASN/A
SolubilityWater, DMSO, DMF
AppearanceBlue solid
Storage Conditions-20°C. Desiccate
Shipping ConditionsAmbient temperature

Abs/Em Spectra

Cy5.5

Documents

FAQs

150 slides has 0.24 µmol (depending on molecular weight this is ~200 µg)
1500 slides has 2.4 µmol (depending on molecular weight this is ~2 mg)

Here is a general protocol for the 150 slide unit size. To prepare 100x stock solution, we recommend reconstituting with 150 ul of DMSO. Storage once in solution: 100X stock solution in DMSO can be stored for 6 months at 2-8 °C protected from light. Avoid moisture.
Working solutions can be made fresh by adding 1 part 100X stock solution, 1 part 0.15% H202, and 100 part buffer.
For example, you can add 5 µL of 100X Tyramide stock solution and 5 µL 0.15% H2O2 to 500 µL of buffer. The final concentration of H2O2 is 0.0015%.
Typical reaction time following the HRP reagent is 2-10 minutes but should be optimized for each workflow. Following the TSA working solution incubation, wash thoroughly.

The post AZDye 680 Tyramide appeared first on VectorLabs.

]]> AZDye 555 Tyramide https://staging.vectorlabs.com/products/azdye-555-tyramide/ Tue, 19 Sep 2023 20:10:38 +0000 https://staging.vectorlabs.com/?post_type=product&p=22515 Description

Orange fluorescent reagent widely used for tyramide signal amplification (TSA) in IHC, ICC, FISH and multicolor FISH. Tyramide signal amplification (TSA) has proven to be a particularly versatile and powerful enzyme amplification technique with improved assay sensitivity. TSA is based on the ability of HRP, in the presence of low concentrations of hydrogen peroxide, to convert labeled tyramine-containing substrate into an oxidized, highly reactive free radical that can covalently bind to tyrosine residues at or near the HRP.

AZDye™ 555 Tyramide (Alexa Fluor® 555 Tyramide equivalent) is a component of many tyramide signal amplification kits, including SuperBoost®. This reagent can be used with any antibody conjugated to HRP for tyramide signal amplification for detection of low abundance targets in multiplexable fluorescent immunocytochemistry (ICC), immunohistochemistry (IHC ), and in situ hybridization (ISH).

[caption id="attachment_25595" align="alignnone" width="1000"] Abs/Em Spectra[/caption]

Specifications

Unit Size 150 slides, 1500 slides
Abs/Em Maxima 555/572 nm
Extinction Coefficient
155,000
Flow Cytometry Laser Line
532 nm or 555 nm
Microscopy Laser Line
532 nm or 555 nm
Spectrally Similar Dyes Alexa Fluor® 555, CF® 555, DyLight® 549, Cy3 Dye
Molecular weight 966.16 (protonated)
CAS N/A
Solubility Water, DMSO, DMF
Appearance Red solid
Storage Conditions -20°C. Desiccate
Shipping Conditions Ambient temperature

The post AZDye 555 Tyramide appeared first on VectorLabs.

]]>

Description

Orange fluorescent reagent widely used for tyramide signal amplification (TSA) in IHC, ICC, FISH and multicolor FISH. Tyramide signal amplification (TSA) has proven to be a particularly versatile and powerful enzyme amplification technique with improved assay sensitivity. TSA is based on the ability of HRP, in the presence of low concentrations of hydrogen peroxide, to convert labeled tyramine-containing substrate into an oxidized, highly reactive free radical that can covalently bind to tyrosine residues at or near the HRP.

AZDye™ 555 Tyramide (Alexa Fluor® 555 Tyramide equivalent) is a component of many tyramide signal amplification kits, including SuperBoost®. This reagent can be used with any antibody conjugated to HRP for tyramide signal amplification for detection of low abundance targets in multiplexable fluorescent immunocytochemistry (ICC), immunohistochemistry (IHC ), and in situ hybridization (ISH).

Specifications

Unit Size150 slides, 1500 slides
Abs/Em Maxima555/572 nm
Extinction Coefficient
155,000
Flow Cytometry Laser Line
532 nm or 555 nm
Microscopy Laser Line
532 nm or 555 nm
Spectrally Similar DyesAlexa Fluor® 555, CF® 555, DyLight® 549, Cy3 Dye
Molecular weight966.16 (protonated)
CASN/A
SolubilityWater, DMSO, DMF
AppearanceRed solid
Storage Conditions-20°C. Desiccate
Shipping ConditionsAmbient temperature

Abs/Em Spectra

AF555

FAQs

150 slides has 0.24 µmol (depending on molecular weight this is ~200 µg)
1500 slides has 2.4 µmol (depending on molecular weight this is ~2 mg)

Here is a general protocol for the 150 slide unit size. To prepare 100x stock solution, we recommend reconstituting with 150 ul of DMSO. Storage once in solution: 100X stock solution in DMSO can be stored for 6 months at 2-8 °C protected from light. Avoid moisture.
Working solutions can be made fresh by adding 1 part 100X stock solution, 1 part 0.15% H202, and 100 part buffer.
For example, you can add 5 µL of 100X Tyramide stock solution and 5 µL 0.15% H2O2 to 500 µL of buffer. The final concentration of H2O2 is 0.0015%.
Typical reaction time following the HRP reagent is 2-10 minutes but should be optimized for each workflow. Following the TSA working solution incubation, wash thoroughly.

The post AZDye 555 Tyramide appeared first on VectorLabs.

]]> AZDye 647 Tyramide https://staging.vectorlabs.com/products/azdye-647-tyramide/ Tue, 19 Sep 2023 20:10:12 +0000 https://staging.vectorlabs.com/?post_type=product&p=22511 Description

Far-red fluorescent reagent widely used for tyramide signal amplification (TSA) in IHC, ICC, FISH and multicolor FISH. Tyramide signal amplification (TSA) has proven to be a particularly versatile and powerful enzyme amplification technique with improved assay sensitivity. TSA is based on the ability of HRP, in the presence of low concentrations of hydrogen peroxide, to convert labeled tyramine-containing substrate into an oxidized, highly reactive free radical that can covalently bind to tyrosine residues at or near the HRP.

AZDye™ 647 Tyramide is a component of many tyramide signal amplification kits, including SuperBoost®. This reagent can be used with any antibody conjugated to HRP for tyramide signal amplification for detection of low abundance targets in multiplexable fluorescent immunocytochemistry (ICC), immunohistochemistry (IHC ), and in situ hybridization (ISH).

[caption id="attachment_25905" align="alignnone" width="1000"] Abs/Em Spectra[/caption]

Specifications

Unit Size 150 slides, 1500 slides
Abs/Em Maxima 648/671 nm
Extinction Coefficient
270,000
Flow Cytometry Laser Line
633 nm or 647 nm
Microscopy Laser Line
633 nm or 647 nm
Spectrally Similar Dyes Alexa Fluor® 647, CF® 647, DyLight® 649
Molecular weight 992.20 (protonated)
CAS N/A
Solubility Water, DMSO, DMF
Appearance Blue solid
Storage Conditions -20°C. Desiccate
Shipping Conditions Ambient temperature

The post AZDye 647 Tyramide appeared first on VectorLabs.

]]>

Description

Far-red fluorescent reagent widely used for tyramide signal amplification (TSA) in IHC, ICC, FISH and multicolor FISH. Tyramide signal amplification (TSA) has proven to be a particularly versatile and powerful enzyme amplification technique with improved assay sensitivity. TSA is based on the ability of HRP, in the presence of low concentrations of hydrogen peroxide, to convert labeled tyramine-containing substrate into an oxidized, highly reactive free radical that can covalently bind to tyrosine residues at or near the HRP.

AZDye™ 647 Tyramide is a component of many tyramide signal amplification kits, including SuperBoost®. This reagent can be used with any antibody conjugated to HRP for tyramide signal amplification for detection of low abundance targets in multiplexable fluorescent immunocytochemistry (ICC), immunohistochemistry (IHC ), and in situ hybridization (ISH).

Specifications

Unit Size150 slides, 1500 slides
Abs/Em Maxima648/671 nm
Extinction Coefficient
270,000
Flow Cytometry Laser Line
633 nm or 647 nm
Microscopy Laser Line
633 nm or 647 nm
Spectrally Similar DyesAlexa Fluor® 647, CF® 647, DyLight® 649
Molecular weight992.20 (protonated)
CASN/A
SolubilityWater, DMSO, DMF
AppearanceBlue solid
Storage Conditions-20°C. Desiccate
Shipping ConditionsAmbient temperature

Abs/Em Spectra

AF647

Documents

FAQs

150 slides has 0.24 µmol (depending on molecular weight this is ~200 µg)
1500 slides has 2.4 µmol (depending on molecular weight this is ~2 mg)

Here is a general protocol for the 150 slide unit size. To prepare 100x stock solution, we recommend reconstituting with 150 ul of DMSO. Storage once in solution: 100X stock solution in DMSO can be stored for 6 months at 2-8 °C protected from light. Avoid moisture.
Working solutions can be made fresh by adding 1 part 100X stock solution, 1 part 0.15% H202, and 100 part buffer.
For example, you can add 5 µL of 100X Tyramide stock solution and 5 µL 0.15% H2O2 to 500 µL of buffer. The final concentration of H2O2 is 0.0015%.
Typical reaction time following the HRP reagent is 2-10 minutes but should be optimized for each workflow. Following the TSA working solution incubation, wash thoroughly.

The post AZDye 647 Tyramide appeared first on VectorLabs.

]]> AZDye 594 Tyramide https://staging.vectorlabs.com/products/azdye-594-tyramide/ Tue, 19 Sep 2023 20:10:11 +0000 https://staging.vectorlabs.com/?post_type=product&p=22508 Description

Red fluorescent reagent widely used for tyramide signal amplification (TSA) in IHC, ICC, FISH and multicolor FISH. Tyramide signal amplification (TSA) has proven to be a particularly versatile and powerful enzyme amplification technique with improved assay sensitivity. TSA is based on the ability of HRP, in the presence of low concentrations of hydrogen peroxide, to convert labeled tyramine-containing substrate into an oxidized, highly reactive free radical that can covalently bind to tyrosine residues at or near the HRP.

AZDye™ 594 Tyramide is a component of many tyramide signal amplification kits, including SuperBoost®. This reagent can be used with any antibody conjugated to HRP for tyramide signal amplification for detection of low abundance targets in multiplexable fluorescent immunocytochemistry (ICC), immunohistochemistry (IHC ), and in situ hybridization (ISH).

[caption id="attachment_25875" align="alignnone" width="1000"] Abs/Em Spectra[/caption]

Specifications

Unit Size 150 slides, 1500 slides
Abs/Em Maxima 590/617 nm
Extinction Coefficient
88,000
Flow Cytometry Laser Line
561 nm or 594 nm
Microscopy Laser Line
594 nm
Spectrally Similar Dyes Alexa Fluor® 594, CF® 594, DyLight® 594
Molecular weight 841.95 (protonated)
CAS N/A
Solubility Water, DMSO, DMF
Appearance Dark blue solid
Storage Conditions -20°C. Desiccate
Shipping Conditions Ambient temperature

The post AZDye 594 Tyramide appeared first on VectorLabs.

]]>

Description

Red fluorescent reagent widely used for tyramide signal amplification (TSA) in IHC, ICC, FISH and multicolor FISH. Tyramide signal amplification (TSA) has proven to be a particularly versatile and powerful enzyme amplification technique with improved assay sensitivity. TSA is based on the ability of HRP, in the presence of low concentrations of hydrogen peroxide, to convert labeled tyramine-containing substrate into an oxidized, highly reactive free radical that can covalently bind to tyrosine residues at or near the HRP.

AZDye™ 594 Tyramide is a component of many tyramide signal amplification kits, including SuperBoost®. This reagent can be used with any antibody conjugated to HRP for tyramide signal amplification for detection of low abundance targets in multiplexable fluorescent immunocytochemistry (ICC), immunohistochemistry (IHC ), and in situ hybridization (ISH).

Specifications

Unit Size150 slides, 1500 slides
Abs/Em Maxima590/617 nm
Extinction Coefficient
88,000
Flow Cytometry Laser Line
561 nm or 594 nm
Microscopy Laser Line
594 nm
Spectrally Similar DyesAlexa Fluor® 594, CF® 594, DyLight® 594
Molecular weight841.95 (protonated)
CASN/A
SolubilityWater, DMSO, DMF
AppearanceDark blue solid
Storage Conditions-20°C. Desiccate
Shipping ConditionsAmbient temperature

Abs/Em Spectra

AF594

Documents

FAQs

150 slides has 0.24 µmol (depending on molecular weight this is ~200 µg)
1500 slides has 2.4 µmol (depending on molecular weight this is ~2 mg)

Here is a general protocol for the 150 slide unit size. To prepare 100x stock solution, we recommend reconstituting with 150 ul of DMSO. Storage once in solution: 100X stock solution in DMSO can be stored for 6 months at 2-8 °C protected from light. Avoid moisture.
Working solutions can be made fresh by adding 1 part 100X stock solution, 1 part 0.15% H202, and 100 part buffer.
For example, you can add 5 µL of 100X Tyramide stock solution and 5 µL 0.15% H2O2 to 500 µL of buffer. The final concentration of H2O2 is 0.0015%.
Typical reaction time following the HRP reagent is 2-10 minutes but should be optimized for each workflow. Following the TSA working solution incubation, wash thoroughly.

The post AZDye 594 Tyramide appeared first on VectorLabs.

]]> AZDye 568 Tyramide https://staging.vectorlabs.com/products/azdye-568-tyramide/ Tue, 19 Sep 2023 20:09:42 +0000 https://staging.vectorlabs.com/?post_type=product&p=22503 Description

Red fluorescent reagent widely used for tyramide signal amplification (TSA) in IHC, ICC, FISH and multicolor FISH. Tyramide signal amplification (TSA) has proven to be a particularly versatile and powerful enzyme amplification technique with improved assay sensitivity. TSA is based on the ability of HRP, in the presence of low concentrations of hydrogen peroxide, to convert labeled tyramine-containing substrate into an oxidized, highly reactive free radical that can covalently bind to tyrosine residues at or near the HRP.

AZDye™ 568 Tyramide is a component of many tyramide signal amplification kits, including SuperBoost®. This reagent can be used with any antibody conjugated to HRP for tyramide signal amplification for detection of low abundance targets in multiplexable fluorescent immunocytochemistry (ICC), immunohistochemistry (IHC ), and in situ hybridization (ISH).

[caption id="attachment_25619" align="alignnone" width="1000"] Abs/Em Spectra[/caption]

Specifications

Unit Size 150 slides, 1500 slides
Abs/Em Maxima 578/602 nm
Extinction Coefficient
88,000
Flow Cytometry Laser Line
532 nm or 568 nm
Microscopy Laser Line
532 nm or 568 nm
Spectrally Similar Dyes Alexa Fluor® 568, CF® 568
Molecular weight 813.89
CAS N/A
Solubility Water, DMSO, DMF
Appearance Dark blue solid
Storage Conditions -20°C. Desiccate
Shipping Conditions Ambient temperature

The post AZDye 568 Tyramide appeared first on VectorLabs.

]]>

Description

Red fluorescent reagent widely used for tyramide signal amplification (TSA) in IHC, ICC, FISH and multicolor FISH. Tyramide signal amplification (TSA) has proven to be a particularly versatile and powerful enzyme amplification technique with improved assay sensitivity. TSA is based on the ability of HRP, in the presence of low concentrations of hydrogen peroxide, to convert labeled tyramine-containing substrate into an oxidized, highly reactive free radical that can covalently bind to tyrosine residues at or near the HRP.

AZDye™ 568 Tyramide is a component of many tyramide signal amplification kits, including SuperBoost®. This reagent can be used with any antibody conjugated to HRP for tyramide signal amplification for detection of low abundance targets in multiplexable fluorescent immunocytochemistry (ICC), immunohistochemistry (IHC ), and in situ hybridization (ISH).

Specifications

Unit Size150 slides, 1500 slides
Abs/Em Maxima578/602 nm
Extinction Coefficient
88,000
Flow Cytometry Laser Line
532 nm or 568 nm
Microscopy Laser Line
532 nm or 568 nm
Spectrally Similar DyesAlexa Fluor® 568, CF® 568
Molecular weight813.89
CASN/A
SolubilityWater, DMSO, DMF
AppearanceDark blue solid
Storage Conditions-20°C. Desiccate
Shipping ConditionsAmbient temperature

Abs/Em Spectra

AF568

Documents

FAQs

150 slides has 0.24 µmol (depending on molecular weight this is ~200 µg)
1500 slides has 2.4 µmol (depending on molecular weight this is ~2 mg)

Here is a general protocol for the 150 slide unit size. To prepare 100x stock solution, we recommend reconstituting with 150 ul of DMSO. Storage once in solution: 100X stock solution in DMSO can be stored for 6 months at 2-8 °C protected from light. Avoid moisture.
Working solutions can be made fresh by adding 1 part 100X stock solution, 1 part 0.15% H202, and 100 part buffer.
For example, you can add 5 µL of 100X Tyramide stock solution and 5 µL 0.15% H2O2 to 500 µL of buffer. The final concentration of H2O2 is 0.0015%.
Typical reaction time following the HRP reagent is 2-10 minutes but should be optimized for each workflow. Following the TSA working solution incubation, wash thoroughly.

The post AZDye 568 Tyramide appeared first on VectorLabs.

]]> AZDye 546 Tyramide https://staging.vectorlabs.com/products/azdye-546-tyramide/ Tue, 19 Sep 2023 20:09:41 +0000 https://staging.vectorlabs.com/?post_type=product&p=22500 Description

Orange fluorescent reagent widely used for tyramide signal amplification (TSA) in IHC, ICC, FISH and multicolor FISH. Tyramide signal amplification (TSA) has proven to be a particularly versatile and powerful enzyme amplification technique with improved assay sensitivity. TSA is based on the ability of HRP, in the presence of low concentrations of hydrogen peroxide, to convert labeled tyramine-containing substrate into an oxidized, highly reactive free radical that can covalently bind to tyrosine residues at or near the HRP.

AZDye™ 546 Tyramide (Alexa Fluor® 546 Tyramide equivalent) is a component of many tyramide signal amplification kits, including SuperBoost®. This reagent can be used with any antibody conjugated to HRP for tyramide signal amplification for detection of low abundance targets in multiplexable fluorescent immunocytochemistry (ICC), immunohistochemistry (IHC ), and in situ hybridization (ISH).

[caption id="attachment_25018" align="alignnone" width="1000"] Abs/Em Spectra[/caption]

Specifications

Unit Size 150 slides, 1500 slides
Abs/Em Maxima 543/563 nm
Extinction Coefficient
105,000
Flow Cytometry Laser Line
532 nm
Microscopy Laser Line
543 or 546 nm
Spectrally Similar Dyes Alexa Fluor® 546, Atto™ 543, CF® 543
Molecular weight 939.08
CAS N/A
Solubility Water, DMSO, DMF, MeOH
Appearance Red solid
Storage Conditions -20°C. Desiccate
Shipping Conditions Ambient temperature

The post AZDye 546 Tyramide appeared first on VectorLabs.

]]>

Description

Orange fluorescent reagent widely used for tyramide signal amplification (TSA) in IHC, ICC, FISH and multicolor FISH. Tyramide signal amplification (TSA) has proven to be a particularly versatile and powerful enzyme amplification technique with improved assay sensitivity. TSA is based on the ability of HRP, in the presence of low concentrations of hydrogen peroxide, to convert labeled tyramine-containing substrate into an oxidized, highly reactive free radical that can covalently bind to tyrosine residues at or near the HRP.

AZDye™ 546 Tyramide (Alexa Fluor® 546 Tyramide equivalent) is a component of many tyramide signal amplification kits, including SuperBoost®. This reagent can be used with any antibody conjugated to HRP for tyramide signal amplification for detection of low abundance targets in multiplexable fluorescent immunocytochemistry (ICC), immunohistochemistry (IHC ), and in situ hybridization (ISH).

Specifications

Unit Size150 slides, 1500 slides
Abs/Em Maxima543/563 nm
Extinction Coefficient
105,000
Flow Cytometry Laser Line
532 nm
Microscopy Laser Line
543 or 546 nm
Spectrally Similar DyesAlexa Fluor® 546, Atto™ 543, CF® 543
Molecular weight939.08
CASN/A
SolubilityWater, DMSO, DMF, MeOH
AppearanceRed solid
Storage Conditions-20°C. Desiccate
Shipping ConditionsAmbient temperature

Abs/Em Spectra

MB543

Documents

FAQs

150 slides has 0.24 µmol (depending on molecular weight this is ~200 µg)
1500 slides has 2.4 µmol (depending on molecular weight this is ~2 mg)

Here is a general protocol for the 150 slide unit size. To prepare 100x stock solution, we recommend reconstituting with 150 ul of DMSO. Storage once in solution: 100X stock solution in DMSO can be stored for 6 months at 2-8 °C protected from light. Avoid moisture.
Working solutions can be made fresh by adding 1 part 100X stock solution, 1 part 0.15% H202, and 100 part buffer.
For example, you can add 5 µL of 100X Tyramide stock solution and 5 µL 0.15% H2O2 to 500 µL of buffer. The final concentration of H2O2 is 0.0015%.
Typical reaction time following the HRP reagent is 2-10 minutes but should be optimized for each workflow. Following the TSA working solution incubation, wash thoroughly.

The post AZDye 546 Tyramide appeared first on VectorLabs.

]]> AZDye 488 Tyramide https://staging.vectorlabs.com/products/azdye-488-tyramide/ Tue, 19 Sep 2023 20:09:38 +0000 https://staging.vectorlabs.com/?post_type=product&p=22494 Description

Green fluorescent reagent widely used for tyramide signal amplification (TSA) in IHC, ICC, FISH and multicolor FISH. Tyramide signal amplification (TSA) has proven to be a particularly versatile and powerful enzyme amplification technique with improved assay sensitivity. TSA is based on the ability of HRP, in the presence of low concentrations of hydrogen peroxide, to convert labeled tyramine-containing substrate into an oxidized, highly reactive free radical that can covalently bind to tyrosine residues at or near the HRP.

AZDye™ 488 Tyramide is a component of many tyramide signal amplification kits, including SuperBoost®. This reagent can be used with any antibody conjugated to HRP for tyramide signal amplification for detection of low abundance targets in multiplexable fluorescent immunocytochemistry (ICC), immunohistochemistry (IHC ), and in situ hybridization (ISH).

[caption id="attachment_25358" align="alignnone" width="1000"] Abs/Em Spectra[/caption]

Specifications

Unit Size 150 slides, 1500 slides
Abs/Em Maxima 494/517 nm
Extinction Coefficient
73,000
Flow Cytometry Laser Line
488 nm
Microscopy Laser Line
488 nm
Spectrally Similar Dyes FAM, Alexa Fluor® 488, Atto™ 488, CF® 488A Dye, DyLight® 488
Molecular weight 653.63 (protonated)
CAS N/A
Solubility Water, DMSO, DMF
Appearance Orange to light red solid
Storage Conditions -20°C. Desiccate
Shipping Conditions Ambient temperature

The post AZDye 488 Tyramide appeared first on VectorLabs.

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Description

Green fluorescent reagent widely used for tyramide signal amplification (TSA) in IHC, ICC, FISH and multicolor FISH. Tyramide signal amplification (TSA) has proven to be a particularly versatile and powerful enzyme amplification technique with improved assay sensitivity. TSA is based on the ability of HRP, in the presence of low concentrations of hydrogen peroxide, to convert labeled tyramine-containing substrate into an oxidized, highly reactive free radical that can covalently bind to tyrosine residues at or near the HRP.

AZDye™ 488 Tyramide is a component of many tyramide signal amplification kits, including SuperBoost®. This reagent can be used with any antibody conjugated to HRP for tyramide signal amplification for detection of low abundance targets in multiplexable fluorescent immunocytochemistry (ICC), immunohistochemistry (IHC ), and in situ hybridization (ISH).

Specifications

Unit Size150 slides, 1500 slides
Abs/Em Maxima494/517 nm
Extinction Coefficient
73,000
Flow Cytometry Laser Line
488 nm
Microscopy Laser Line
488 nm
Spectrally Similar DyesFAM, Alexa Fluor® 488, Atto™ 488, CF® 488A Dye, DyLight® 488
Molecular weight653.63 (protonated)
CASN/A
SolubilityWater, DMSO, DMF
AppearanceOrange to light red solid
Storage Conditions-20°C. Desiccate
Shipping ConditionsAmbient temperature

Abs/Em Spectra

AF488

Documents

FAQs

150 slides has 0.24 µmol (depending on molecular weight this is ~200 µg)
1500 slides has 2.4 µmol (depending on molecular weight this is ~2 mg)

Here is a general protocol for the 150 slide unit size. To prepare 100x stock solution, we recommend reconstituting with 150 ul of DMSO. Storage once in solution: 100X stock solution in DMSO can be stored for 6 months at 2-8 °C protected from light. Avoid moisture.
Working solutions can be made fresh by adding 1 part 100X stock solution, 1 part 0.15% H202, and 100 part buffer.
For example, you can add 5 µL of 100X Tyramide stock solution and 5 µL 0.15% H2O2 to 500 µL of buffer. The final concentration of H2O2 is 0.0015%.
Typical reaction time following the HRP reagent is 2-10 minutes but should be optimized for each workflow. Following the TSA working solution incubation, wash thoroughly.

The post AZDye 488 Tyramide appeared first on VectorLabs.

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