Tetrazines demonstrate exceptionally fast cycloaddition kinetics (up to 30 000 M^-1 s^-1) with trans-cyclooctenes (TCO) as the dienophile, the fastest kinetics ever reported for any bioorthogonal reaction. In addition, inverse-Electron-Demand Diels-Alder reaction of tetrazines trans-cyclooctenea forms a stable covalent bond and does not require Cu-catalyst or elevated temperatures.

AZDye 660R is a bright and photostable far-red dye that emits fluorescence at about 685 nm in the borderline spectral region between far-red and near-IR. Although the absorption maximum is at around 665 nm, this dye can be sufficiently excited by the 633 or 635 nm laser. AZDye 660R dye is water soluble and pH-insensitive from pH 4 to pH 10. AZDye 660R is a rhodamine-based dye, and like rhodamine dyes in general, it is exceptionally photostable (Figure 1). The superior photostability and excellent brightness of AZDye 660R make the dye an ideal choice for confocal microscopy and other demanding applications.

[caption id="attachment_26345" align="alignnone" width="1000"] Abs/Em Spectra[/caption]

Specifications

The post AZDye 660R Tetrazine appeared first on VectorLabs.

Terazine-activated Cy5 dye reacts with TCO-containing compounds via a Inverse-Electron-Demand Diels-Alder reaction to form a stable covalent bond and does not require Cu-catalyst or elevated temperatures. The inverse-electron demand Diels-Alder cycloaddition reaction of TCO with tetrazines is a bioorthogonal reaction that possesses exceptional kinetics and selectivity. Such excellent reaction rate constants are unparalleled by any other bioorthogonal reaction pair described to date.

Cy5 Tetrazine is a water-soluble, pH-insensitive from pH 4 to pH 10, far-red-fluorescent probe with excitation ideally suited for the 633 nm or 647 nm laser lines. Its absorption and emission spactra are almost identical to those of Alexa Fluor® 647, CF® 647 Dye, or any other Cyanine5 based fluorescent dyes.

This is sulfonated dye is also known as sulfo-Cyanine5.

[caption id="attachment_24122" align="alignnone" width="1200"] Abs/Em Spectra[/caption]

Specifications

The post Cy5 Tetrazine appeared first on VectorLabs.

Terazine-activated TAMRA dye reacts with TCO-containing compounds via a Inverse-Electron-Demand Diels-Alder reaction to form a stable covalent bond and does not require Cu-catalyst or elevated temperatures. The inverse-electron demand Diels-Alder cycloaddition reaction of TCO with tetrazines is a bioorthogonal reaction that possesses exceptional kinetics and selectivity. Such excellent reaction rate constants are unparalleled by any other bioorthogonal reaction pair described to date.

Abs/Em Spectra

Specifications

The post TAMRA Tetrazine appeared first on VectorLabs.

Tetrazines demonstrate exceptionally fast cycloaddition kinetics (up to 30 000 M^-1 s^-1) with trans-cyclooctenes (TCO) as the dienophile, the fastest kinetics ever reported for any bioorthogonal reaction. In addition, inverse-Electron-Demand Diels-Alder reaction of tetrazines trans-cyclooctenea forms a stable covalent bond and does not require Cu-catalyst or elevated temperatures. In applications such as in vivo cancer imaging or pre‐targeted cell labeling studies where rapid reaction kinetics are desired, a faster hydrogen substituted Cy®3 Tetrazine probe would be a probe of choice.

Cy®3 is a bright, water-soluble, and pH insensitive orange-fluorescent dye that can be excited using the 532 nm or 555 nm laser line and visualized with TRITC (tetramethylrhodamine) filter sets. Cy3 conjugates give less background than TAMRA and most other commonly used fluorescent dyes.

Cy®Dye is a trademark of GE Healthcare.

[caption id="attachment_24113" align="alignnone" width="1000"] Abs/Em Spectra[/caption]

Specifications

The post Cy3 Tetrazine appeared first on VectorLabs.

A bright, red-fluorescent probe used for detection TCO-tagged biopolymers. AZDye 594 Tetrazine demonstrates exceptionally fast cycloaddition kinetics (up to 30 000 M^-1 s^-1) with trans-cyclooctenes (TCO) as the dienophile, the fastest kinetics ever reported for any bioorthogonal reaction. In applications such as in vivo cancer imaging or pre‐targeted cell labeling studies where rapid reaction kinetics is a must AZDye 594 Tetrazine probe would of great value.

[caption id="attachment_25875" align="alignnone" width="1000"] Abs/Em Spectra[/caption]

Specifications

The post AZDye 594 Tetrazine appeared first on VectorLabs.

A bright, green-fluorescent probe used for detection TCO-tagged biopolymers. AZDye 488 Tetrazine demonstrates exceptionally fast cycloaddition kinetics (up to 30 000 M^-1 s^-1) with trans-cyclooctenes (TCO) as the dienophile, the fastest kinetics ever reported for any bioorthogonal reaction. In applications such as in vivo cancer imaging or pre‐targeted cell labeling studies where rapid reaction kinetics is a must AZDye 488 Tetrazine probe would of great value.

[caption id="attachment_25358" align="alignnone" width="1000"] Abs/Em Spectra[/caption]

Specifications

The post AZDye 488 Tetrazine appeared first on VectorLabs.