Specifications

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Specifications

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AZDye 680 (Alexa Fluor® 680 equivalent) is a bright and photostable near-IR dye that is spectrally identical to Alexa Fluor® 680 and IRDye® 680RD. AZDye 680 is often used for small animal imaging applications in the 700 nm wavelength and provides excellent clearance profiles in animals. Flow cytometry is another common application for this dye. AZDye 680 ideally suited for the 633 nm laser line.

A probe for copper-less azide detection (AZDye™ 680 DBCO) is also available for application where the presence of copper is not acceptable.

[caption id="attachment_24263" align="alignnone" width="1940"] Abs/Em Spectra[/caption]

Specifications

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AZDye™ 633 Alkyne is a bright and photostable far-red fluorescent probe with excitation ideally suited to the 633 nm or 635 nm laser excitation source. AZDye™ 633 DBCO is water-soluble, pH-insensitive from pH 4 to pH 10. AZDye™ 633 spectrally is almost identical to Alexa Fluor® 633, DyLight® 633 or CF® 633 Dye. Combination of superior brightness and very low autofluorescence background signal in most biological samples in far-red spectral region allows for very sensitive detection of azide-labeled biomolecules using AZDye™ 633 Alkyne.

[caption id="attachment_27033" align="alignnone" width="1000"] Abs/Em Spectra[/caption]

Specifications

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MB 660R Alkyne is often a reagent of choice for imaging of low abundance azide-containing biomolecules. AZDye™ 647 Alkyne reacts with azides via a copper-catalyzed click reaction (CuAAC) to form a stable triazole linker. The brightness and photostability of this dye are best suited to direct imaging of low-abundance targets.

MB 660R is a bright and photostable far-red dye that emits fluorescence at about 685 nm in the borderline spectral region between far-red and near-IR. Although the absorption maximum is at around 665 nm, this dye can be sufficiently excited by the 633 or 635 nm laser. MB 660R dye is water soluble and pH-insensitive from pH 4 to pH 10. MB 660R is a rhodamine-based dye, and like rhodamine dyes in general, it is exceptionally photostable (Figure 1). The superior photostability and excellent brightness of MB 660R make the dye an ideal choice for confocal microscopy and other demanding applications.

MB 660R dye spectrally is almost identical to Alexa Fluor® 660 and CF® 660R Dye and can be used a less expensive alternative to these dyes.

Alexa Fluor® is a registered trademark of Thermo Fisher Scientific. CF® Dye is a registered trademark of Biotium.

[caption id="attachment_26345" align="alignnone" width="1000"] Abs/Em Spectra[/caption]

Specifications

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The red-fluorescent TAMRA Azide (TAMRA-PEG4-Alkyne) can be reacted with azides via a copper-catalyzed click reaction (CuAAC). TAMRA (tetramethylrhodamine) is a bright fluorescent label is compatible with various excitation sources including mercury arc, tungsten and xenon arc lamps, the 544 nm line of the Helium-Neon laser and the 532 nm green laser line.

[caption id="attachment_24144" align="alignnone" width="1000"] Abs/Em Spectra[/caption]

Specifications

The post TAMRA Alkyne appeared first on VectorLabs.

OG 488 Alkyne, (also known as Oregon Green® 488 Alkyne or 2′,7′-Difluorocarboxyfluorescein Alkyne, 6-isomer) is a bright, green-fluorescent alkyne-activated probe routinely used for imaging of azide-containing biomolecules. OG 488 Alkyne reacts with azides via a copper-catalyzed click reaction (CuAAC) to form a stable triazole linker.

The fluorinated carboxyfluoresceins have higher photostability and ionize at a lower pH (pKa 4.8) than fluorescein (pKa 6.5), which makes them superior fluorescent dyes for use as reporter molecules in biological systems. The absorption and emission of OG 488 dye are virtually identical to widely used fluorescein dyes.

The combination of higher photostability, lower pKa, and excitation ideally suited to the 488 nm laser line makes OG 488 dye an ideal replacement for the widely used fluorescein dyes.

Oregon Green® is a registered trademark of Thermo Fisher Scientific.

[caption id="attachment_25358" align="alignnone" width="1000"] Abs/Em Spectra[/caption]

Specifications

The post OG 488 Alkyne appeared first on VectorLabs.

AZDye™ 647 Alkyne is a water-soluble, pH-insensitive from pH 4 to pH 10, far-red-fluorescent probe with excitation ideally suited for the 633 nm or 647 nm laser lines, and visualized with Cy5 filter sets.

AZDye™ 647 is often a reagent of choice for imaging of low abundance azide-containing biomolecules. AZDye™ 647 Alkyne reacts with azides via a copper-catalyzed click reaction (CuAAC) to form a stable triazole linker.

AZDye™ 647 is structurally similar to Alexa Fluor® 647, and spectrally is almost identical to Cy5 Dye, Alexa Fluor® 647, CF® 647 Dye, or any other Cyanine5 based fluorescent dyes. AZDye™ 647 Azide can be used as a less expensive alternative to Alexa Fluor® 647 Azide.

For application where the presence of copper is not acceptable, please consider our AZDye™ 647 DBCO or Cy5 DBCO probes for copper-less detection of azide-modified molecules.

Alexa Fluor® is a registered trademark of Thermo Fisher Scientific.

[caption id="attachment_25905" align="alignnone" width="1000"] Abs/Em Spectra[/caption]

Specifications

The post AZDye 647 Alkyne appeared first on VectorLabs.

AZDye™ 594 Alkyne is a bright, red-fluorescent alkyne-activated probe routinely used for imaging of azide-containing biomolecules. AZDye™ 594 Alkyne reacts with azides via a copper-catalyzed click reaction (CuAAC) to form a stable triazole linker.

AZDye™ 594 is bright, water-soluble, and pH-insensitive from pH 4 to pH 10 red-fluorescent dye with absorption and emission maxima at 590 and 617 nm, respectively. It can be used with the 561 nm and 594 nm laser lines. AZDye™ 594 dye conjugated to a variety of antibodies, peptides, proteins, tracers, and amplification substrates often used for generation of stable signal in imaging and flow cytometry. AZDye™ 594 Azide dye structurally is identical to Alexa Fluor® 594 Azide. Its absorption/emission spectra is a perfect match to spectra of many other fluorescent dyes based on sulfonated rhodamine core, including CF®594 Dye, DyLight® 594 and Alexa Fluor® 594.

For application where the presence of copper is not acceptable, please consider our AZDye™ 594 DBCO probe for copper-less detection of azide-modified molecules.

[caption id="attachment_25875" align="alignnone" width="1000"] Abs/Em Spectra[/caption]

Specifications

The post AZDye 594 Alkyne appeared first on VectorLabs.

AZDye™ 568 Alkyne is a bright, orange-fluorescent alkyne-activated probe routinely used for imaging of azide-containing biomolecules. AZDye™ 568 Alkyne reacts with azides via a copper-catalyzed click reaction (CuAAC) to form a stable triazole linker.

AZDye 568™ is a bright, and highly photostable, orange-fluorescent probe optimally excited by the 568 nm laser line on the Ar-Kr mixed-gas laser. This probe is water-soluble and its fluorescence is pH independent over a wide pH range. The brightness and photostability of this dye are best suited to direct imaging of low-abundance targets. AZDye™ 568 dye structurally is identical to Alexa Fluor® 568 Dye. Its absorption/emission spectra is a perfect match to spectra of many other fluorescent dyes based on sulfonated rhodamine core, including CF® 568 Dye and Alexa Fluor® 568.

For application where the presence of copper is not acceptable, please consider our AZDye™ 568 DBCO probes for copper-less detection of azide-modified molecules.

Alexa Fluor® is a registered trademark of Thermo Fisher Scientific.

[caption id="attachment_25619" align="alignnone" width="1000"] Abs/Em Spectra[/caption]

Specifications

The post AZDye 568 Alkyne appeared first on VectorLabs.