CCT-1336 – VectorLabs https://staging.vectorlabs.com From linker design and synthesis, bioconjugated design and manufacturing, biomolecule labeling and functionalization, and detection system and solutions, our team is here to help you move forward, with impact. Wed, 22 Oct 2025 17:11:35 +0000 en-US hourly 1 https://wordpress.org/?v=6.9 https://staging.vectorlabs.com/wp-content/uploads/2024/11/V-favicon-02-100x100.png CCT-1336 – VectorLabs https://staging.vectorlabs.com 32 32 AZDye 350 NHS Ester https://staging.vectorlabs.com/products/azdye-350-nhs-ester/ Tue, 19 Sep 2023 23:14:27 +0000 https://staging.vectorlabs.com/?post_type=product&p=23457 Description

AZDye™ 350 NHS Ester (Alexa Fluor® 350 NHS Ester equivalent) is an amine reactive, water-soluble, blue-emitting dye used to specifically and efficiently modify a primary amine (e.g., side chain of lysine residues or aminosilane-coated surfaces) at pH 7-9 to form a stable, covalent amide bond. The NHS ester (or succinimidyl ester) is the most popular tool for conjugating dyes to the primary amines of proteins or antibodies (Lys), amine-modified oligonucleotides, and other amine-containing molecules.

AZDye™ 350 is a moderately photostable, blue-fluorescent probe optimally excited by the 350 nm laser line routinely used for generation of stable signal in imaging and flow cytometry. The brightness and photostability of blue dyes are best suited to direct imaging of high-abundance targets.

AZDye™ 350 NHS Ester is structurally identical to Alexa Fluor® 350 NHS Ester.

[caption id="attachment_23916" align="alignnone" width="1200"] Abs/Em Spectra[/caption]

Specifications

Unit Size 1 mg, 5 mg, 25 mg, 100 mg
Reactivity Primary amines
Abs/Em Maxima 346/445 nm
Extinction coefficient 19,000 cm-1M-1
Solubility Water, DMSO, DMF
Spectrally similar dyes Alexa Fluor® 350, AMCA, DyLight® 350
Molecular weight 410.35
Storage Conditions -20°C. Desiccate
Shipping Conditions Ambient temperature

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Description

350nm laser 1 1AZDye™ 350 NHS Ester (Alexa Fluor® 350 NHS Ester equivalent) is an amine reactive, water-soluble, blue-emitting dye used to specifically and efficiently modify a primary amine (e.g., side chain of lysine residues or aminosilane-coated surfaces) at pH 7-9 to form a stable, covalent amide bond. The NHS ester (or succinimidyl ester) is the most popular tool for conjugating dyes to the primary amines of proteins or antibodies (Lys), amine-modified oligonucleotides, and other amine-containing molecules.

AZDye™ 350 is a moderately photostable, blue-fluorescent probe optimally excited by the 350 nm laser line routinely used for generation of stable signal in imaging and flow cytometry. The brightness and photostability of blue dyes are best suited to direct imaging of high-abundance targets.

AZDye™ 350 NHS Ester is structurally identical to Alexa Fluor® 350 NHS Ester.

Specifications

Unit Size1 mg, 5 mg, 25 mg, 100 mg
ReactivityPrimary amines
Abs/Em Maxima346/445 nm
Extinction coefficient19,000 cm-1M-1
SolubilityWater, DMSO, DMF
Spectrally similar dyesAlexa Fluor® 350, AMCA, DyLight® 350
Molecular weight410.35
Storage Conditions-20°C. Desiccate
Shipping ConditionsAmbient temperature

Abs/Em Spectra

AF350

Protein Labeling Calculator

Enter only one — either volume or amount of protein to be labeled
Enter only one — either maximum concentration of DMSO or volume DMSO added to label

Documents

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