CCT-1115 – VectorLabs https://staging.vectorlabs.com From linker design and synthesis, bioconjugated design and manufacturing, biomolecule labeling and functionalization, and detection system and solutions, our team is here to help you move forward, with impact. Wed, 22 Oct 2025 17:13:19 +0000 en-US hourly 1 https://wordpress.org/?v=6.9 https://staging.vectorlabs.com/wp-content/uploads/2024/11/V-favicon-02-100x100.png CCT-1115 – VectorLabs https://staging.vectorlabs.com 32 32 IR 750 NHS ester https://staging.vectorlabs.com/products/ir-750-nhs-ester/ Tue, 19 Sep 2023 22:32:12 +0000 https://staging.vectorlabs.com/?post_type=product&p=23077 Description

IR 750 NHS Ester (IRDye® 750 NHS Ester analog) is a water-soluble, bright, near-IR fluorescent dye that is spectrally similar to other 750 dyes used for flow cytometry, microscopy and other applications. IR 750 conjugates ideally suited for the 633 nm laser lines or dye-pumped laser. A significant advantage to using long wavelength dyes such as Cy7® Dye or Alexa Fluor® 750 dye over other fluorophores is the low autofluorescence of biological specimens in this region of the spectrum.

The NHS ester (or succinimidyl ester) is the most popular amine reactive group for labeling with the primary amines of proteins (Lys), amine-modified oligonucleotides, and other amine-containing molecules. Proteins can be labeled with IR 750 NHS Ester at high molar ratios without significant self-quenching, leading to brighter conjugates and more sensitive detection. IR 750 conjugates are is pH-insensitive over a wide molar range, helping ensure that you get stable signals in imaging and flow cytometry applications.

Abs/Em Spectra

Specifications

Unit Size 1 mg, 5 mg, 25 mg, 100 mg
Reactivity Primary amine
Abs/Em Maxima 756/776 nm
Extinction coefficient 270,000 cm-1M-1
Solubility Water, DMSO, DMF
Spectrally similar dyes Cy7, Alexa Fluor® 750, IRDye® 750
Molecular weight 1098.28
Storage Conditions -20°C.
Shipping Conditions Ambient temperature

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Description

IR 750 laser

IR 750 NHS Ester (IRDye® 750 NHS Ester analog) is a water-soluble, bright, near-IR fluorescent dye that is spectrally similar to other 750 dyes used for flow cytometry, microscopy and other applications. IR 750 conjugates ideally suited for the 633 nm laser lines or dye-pumped laser. A significant advantage to using long wavelength dyes such as Cy7® Dye or Alexa Fluor® 750 dye over other fluorophores is the low autofluorescence of biological specimens in this region of the spectrum.

The NHS ester (or succinimidyl ester) is the most popular amine reactive group for labeling with the primary amines of proteins (Lys), amine-modified oligonucleotides, and other amine-containing molecules. Proteins can be labeled with IR 750 NHS Ester at high molar ratios without significant self-quenching, leading to brighter conjugates and more sensitive detection. IR 750 conjugates are is pH-insensitive over a wide molar range, helping ensure that you get stable signals in imaging and flow cytometry applications.

Specifications

Unit Size1 mg, 5 mg, 25 mg, 100 mg
ReactivityPrimary amine
Abs/Em Maxima756/776 nm
Extinction coefficient270,000 cm-1M-1
SolubilityWater, DMSO, DMF
Spectrally similar dyesCy7, Alexa Fluor® 750, IRDye® 750
Molecular weight1098.28
Storage Conditions-20°C.
Shipping ConditionsAmbient temperature

Abs/Em Spectra

Cy7

Protein Labeling Calculator

Enter only one — either volume or amount of protein to be labeled
Enter only one — either maximum concentration of DMSO or volume DMSO added to label

Documents

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