Catalog Vectashield Plus – VectorLabs https://staging.vectorlabs.com From linker design and synthesis, bioconjugated design and manufacturing, biomolecule labeling and functionalization, and detection system and solutions, our team is here to help you move forward, with impact. Wed, 22 Oct 2025 17:14:34 +0000 en-US hourly 1 https://wordpress.org/?v=6.9 https://staging.vectorlabs.com/wp-content/uploads/2024/11/V-favicon-02-100x100.png Catalog Vectashield Plus – VectorLabs https://staging.vectorlabs.com 32 32 VECTASHIELD® PLUS Antifade Mounting Medium https://staging.vectorlabs.com/products/vectashield-plus-antifade/ Mon, 01 May 2023 15:29:14 +0000 https://staging.vectorlabs.com/?post_type=product&p=13487 Description

VECTASHIELD PLUS protects against fading across the visible spectrum, even under far-red wavelengths. It is compatible with most commercially available fluorophores. This new formulation of non-setting media improves upon the original VECTASHIELD products. Specifically, VECTASHIELD PLUS exhibits no inherent background or toning and provides superior fluorophore signal retention across the spectrum, including far-red wavelengths.

Features:

  • No inherent toning or background
  • Superior signal retention across the spectrum from blue to far red
  • Mounted sections can be viewed immediately
  • Available in two convenient sizes
  • Superior non-setting formulation

 

Specifications

Unit Size 2 ml, 10 ml
Applications Immunofluorescence, In situ hybridization
Recommended Storage 2-8 ºC
Mounting Aqueous (Non-Hardening)
Antifade Yes
Counterstain None

Technical Information

The refractive index for VECTASHIELD Mounting Medium is 1.45.

VECTASHIELD PLUS Mounting Medium does not solidify, but rather, remains a liquid on the slide—which can then be stored without sealing. If desired, coverslips can be sealed around the perimeter using nail polish or plastic sealant. Mounted slides should be stored at 2-8 °C and protected from light.

VECTASHIELD Mounting Medium Antifade Comparison

Other manufacturers measure the antifade properties of their mountants using labeled microspheres or arrayed spots. Vector Labs prefers to measure antifade properties of VECTASHIELD mountants using frozen tissue sections that have been immunohistochemically stained with fluorescently labeled secondary antibodies. Antifade capability is then measured using a 40x objective with real time imaging over 30 seconds of continuous exposure to the excitation illumination. Individual intensity measurements are recorded from 6 separate labeled regions and the average is calculated. The intensity after 30 second exposure is expressed as a percentage of the intensity at zero time. The values for PG are taken from the manufacturer’s published results.

The post VECTASHIELD® PLUS Antifade Mounting Medium appeared first on VectorLabs.

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Description

VECTASHIELD PLUS protects against fading across the visible spectrum, even under far-red wavelengths. It is compatible with most commercially available fluorophores. This new formulation of non-setting media improves upon the original VECTASHIELD products. Specifically, VECTASHIELD PLUS exhibits no inherent background or toning and provides superior fluorophore signal retention across the spectrum, including far-red wavelengths.

Features:

  • No inherent toning or background
  • Superior signal retention across the spectrum from blue to far red
  • Mounted sections can be viewed immediately
  • Available in two convenient sizes
  • Superior non-setting formulation

Specifications

Unit Size2 ml, 10 ml
ApplicationsImmunofluorescence, In situ hybridization
Recommended Storage2-8 ºC
MountingAqueous (Non-Hardening)
AntifadeYes
CounterstainNone

Documents

Product FAQ

VECTASHIELD PLUS provides several advantages over the regular VECTASHIELD (H-1000 & H-1200). In particular, VECTASHIELD PLUS media have significantly lower inherent background​ toning and provide superior signal retention particularly in the far-red spectrum (e.g. Cy5).

Similar to other non-setting media, we recommend you to seal the coverslip perimeter if the intention is to store the slides for a period of time.

Yes, the antifade mounting media are at least equivalent in signal retention. We have not seen any reduction in performance ​with any fluorophores evaluated, and with some dyes (i.e. Alexa Fluor 647 and Dylight 649) there are advantages. 

We do know that regular VECTASHIELD antifade mounting media (H-1000 & H-1200) are suitable for SRM.​ However, the formulation for VECTASHIELD PLUS is different from that used for ​regular VECTASHIELD. Indeed one component of H-1000 and​ H-1200 that may have been a contributing factor to the underlying blue​ tone and that appears to play a key role in optimal signal for SRM, has​ been removed from VECTASHIELD PLUS. Therefore, until we have data to the ​contrary VECTASHIELD PLUS would not be recommended for SRM.

Technical Information

The refractive index for VECTASHIELD Mounting Medium is 1.45.

VECTASHIELD PLUS Mounting Medium does not solidify, but rather, remains a liquid on the slide—which can then be stored without sealing. If desired, coverslips can be sealed around the perimeter using nail polish or plastic sealant. Mounted slides should be stored at 2-8 °C and protected from light.

VECTASHIELD Mounting Medium Antifade Comparison

Other manufacturers measure the antifade properties of their mountants using labeled microspheres or arrayed spots. Vector Labs prefers to measure antifade properties of VECTASHIELD mountants using frozen tissue sections that have been immunohistochemically stained with fluorescently labeled secondary antibodies. Antifade capability is then measured using a 40x objective with real time imaging over 30 seconds of continuous exposure to the excitation illumination. Individual intensity measurements are recorded from 6 separate labeled regions and the average is calculated. The intensity after 30 second exposure is expressed as a percentage of the intensity at zero time. The values for PG are taken from the manufacturer’s published results.

New Fluorophore Signal Table VECTASHIELD jpg New Fluoro Remaining Graph jpg VS.Chart .v2 jpg

Resources

02 15 23 banner jpg

How to mount a coverslip on a slide

Correctly coverslipping your slides ensures that your tissue specimens are preserved for optimal imaging and viewing. In this blog post, we will walk you through how to properly coverslip your slides to protect your samples.

august 10 banner 1

How to choose antifade mounting media

As far as antifade mounting media go, one-size-fits-all solutions are few and far between. Here, we break down key factors you should consider when choosing the best antifade mounting medium for your experiment.

v2 cover VL H 1900 UserGuide LBL00829 jpg
Step by Step Protocol
v2 VL LIT3018 VECTASHIELD PLUS Brochure Cover 1 jpg
Product-specific information

The post VECTASHIELD® PLUS Antifade Mounting Medium appeared first on VectorLabs.

]]> VECTASHIELD® PLUS Antifade Mounting Medium with DAPI https://staging.vectorlabs.com/products/vectashieldr-plus-antifade-mounting-medium-dapi/ Wed, 26 Apr 2023 03:02:05 +0000 https://staging.vectorlabs.com/?post_type=product&p=12067 Description

VECTASHIELD PLUS Mounting Medium protects against fading across the visible spectrum, even under far-red wavelengths. It is compatible with most commercially available fluorophores. This new formulation of non-setting media improves upon the original VECTASHIELD products. Specifically, VECTASHIELD PLUS exhibits no inherent background or toning and provides superior fluorophore signal retention across the spectrum, including far-red wavelengths.

Features:

  • No inherent toning or background
  • Superior signal retention across the spectrum from blue to far red
  • Mounted sections can be viewed immediately
  • DAPI (blue) counterstain premixed with medium
  • Available in two convenient sizes
  • Superior non-setting formulation

 

VECTASHIELD PLUS Mounting Medium with DAPI does not solidify, but remains a liquid on the slide and can be stored without sealing. If desired, coverslips can be sealed around the perimeter with nail polish or a plastic sealant. Mounted slides should be stored at 2-8 °C, protected from light.

Specifications

Unit Size 2 ml, 10 ml
Applications Immunofluorescence, In situ hybridization
Mounting Aqueous (Non-Hardening)
Antifade Yes
Counterstain DAPI (blue fluorescence)

 

The post VECTASHIELD® PLUS Antifade Mounting Medium with DAPI appeared first on VectorLabs.

]]>

Description

VECTASHIELD PLUS Mounting Medium protects against fading across the visible spectrum, even under far-red wavelengths. It is compatible with most commercially available fluorophores. This new formulation of non-setting media improves upon the original VECTASHIELD products. Specifically, VECTASHIELD PLUS exhibits no inherent background or toning and provides superior fluorophore signal retention across the spectrum, including far-red wavelengths.

Features:

  • No inherent toning or background
  • Superior signal retention across the spectrum from blue to far red
  • Mounted sections can be viewed immediately
  • DAPI (blue) counterstain premixed with medium
  • Available in two convenient sizes
  • Superior non-setting formulation

 

VECTASHIELD PLUS Mounting Medium with DAPI does not solidify, but remains a liquid on the slide and can be stored without sealing. If desired, coverslips can be sealed around the perimeter with nail polish or a plastic sealant. Mounted slides should be stored at 2-8 °C, protected from light.

Specifications

Unit Size2 ml, 10 ml
ApplicationsImmunofluorescence, In situ hybridization
MountingAqueous (Non-Hardening)
AntifadeYes
CounterstainDAPI (blue fluorescence)

Documents

Citations

Technical Information

The refractive index for VECTASHIELD Mounting Medium is 1.45.

VECTASHIELD PLUS Mounting Medium does not solidify, but rather, remains a liquid on the slide ‐ which can then be stored without sealing. If desired, coverslips can be sealed around the perimeter using nail polish or plastic sealant. Mounted slides should be stored at 2-8 °C and protected from light.

VECTASHIELD Mounting Medium Antifade Comparison

Other manufacturers measure the antifade properties of their mountants using labeled microspheres or arrayed spots. Vector Labs prefers to measure antifade properties of VECTASHIELD mountants using frozen tissue sections that have been immunohistochemically stained with fluorescently labeled secondary antibodies. Antifade capability is then measured using a 40x objective with real time imaging over 30 seconds of continuous exposure to the excitation illumination. Individual intensity measurements are recorded from 6 separate labeled regions and the average is calculated. The intensity after 30 second exposure is expressed as a percentage of the intensity at zero time. The values for PG are taken from the manufacturer’s published results.

New Fluorophore Signal Table VECTASHIELD jpg

New Fluoro Remaining Graph jpg

VS.Chart .v2 jpg

Check out the video below on how to use a hydrophobic barrier pen.

02 15 23 banner jpg

How to mount a coverslip on a slide

Correctly coverslipping your slides ensures that your tissue specimens are preserved for optimal imaging and viewing. In this blog post, we will walk you through how to properly coverslip your slides to protect your samples.

august 10 banner 1

How to choose antifade mounting media

As far as antifade mounting media go, one-size-fits-all solutions are few and far between. Here, we break down key factors you should consider when choosing the best antifade mounting medium for your experiment.

v2 cover VL H 2000 UserGuide LBL00830 jpg
Step by Step Protocol
v2 VL LIT3018 VECTASHIELD PLUS Brochure Cover 1 jpg
Product-specific information

The post VECTASHIELD® PLUS Antifade Mounting Medium with DAPI appeared first on VectorLabs.

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