Catalog Hardset – VectorLabs https://staging.vectorlabs.com From linker design and synthesis, bioconjugated design and manufacturing, biomolecule labeling and functionalization, and detection system and solutions, our team is here to help you move forward, with impact. Wed, 22 Oct 2025 17:14:22 +0000 en-US hourly 1 https://wordpress.org/?v=6.9 https://staging.vectorlabs.com/wp-content/uploads/2024/11/V-favicon-02-100x100.png Catalog Hardset – VectorLabs https://staging.vectorlabs.com 32 32 VECTASHIELD® HardSet™ Antifade Mounting Medium with Phalloidin https://staging.vectorlabs.com/products/vectashield-hardset-phalliodin/ Sat, 29 Apr 2023 05:09:34 +0000 https://staging.vectorlabs.com/?post_type=product&p=15172 Description

The VECTASHIELD HardSet Antifade Mounting Medium with TRITC-Phalloidin prevents rapid photobleaching of fluorescent proteins and fluorescent dyes. Fluorescent derivatives of phalloidin are used to stain actin filaments. TRITC (tetramethylrhodamine) is excited at 544 nm and emits at 572 nm, producing an orange-red fluorescence. VECTASHIELD HardSet Mounting Media hardens after coverslipping. After approximately 15 minutes at room temperature, the coverslip will become immobilized, and optimal antifade ability and refractive index will be achieved.

Features:

  • Ideal refractive index (1.46)
  • Ready-to-use – from the refrigerator to the benchtop
  • No warming necessary
  • Can be stored without sealing for long term analysis
  • Hardening formulation

Specifications

Unit Size 10 ml
Applications Immunofluorescence, In situ hybridization, Cellular Imaging
Mounting Aqueous (Hardening)
Antifade Yes
Counterstain Phalloidin Rhodamine (red fluorescence)
Cellular Stain Cytoskeleton

The post VECTASHIELD® HardSet™ Antifade Mounting Medium with Phalloidin appeared first on VectorLabs.

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Description

The VECTASHIELD HardSet Antifade Mounting Medium with TRITC-Phalloidin prevents rapid photobleaching of fluorescent proteins and fluorescent dyes. Fluorescent derivatives of phalloidin are used to stain actin filaments. TRITC (tetramethylrhodamine) is excited at 544 nm and emits at 572 nm, producing an orange-red fluorescence. VECTASHIELD HardSet Mounting Media hardens after coverslipping. After approximately 15 minutes at room temperature, the coverslip will become immobilized, and optimal antifade ability and refractive index will be achieved.

Features:

  • Ideal refractive index (1.46)
  • Ready-to-use – from the refrigerator to the benchtop
  • No warming necessary
  • Can be stored without sealing for long term analysis
  • Hardening formulation

Specifications

Unit Size10 ml
ApplicationsImmunofluorescence, In situ hybridization, Cellular Imaging
MountingAqueous (Hardening)
AntifadeYes
CounterstainPhalloidin Rhodamine (red fluorescence)
Cellular StainCytoskeleton

Documents

Product FAQs

All of the VECTASHIELD formats we currently offer which includes regular non-setting VECTASHIELD, PLUS, HardSet and Vibrance formulations each contain a percentage of glycerol.

No form of tissue dehydration (e.g. air drying or ethanol exposure) is required nor recommended when applying VECTASHIELD. From our experience, the most optimal antifade actions of VECTASHIELD are obtained when the preparation is removed from the final buffer/water rinse, kept slightly wet/moist and then coverslipped with a small volume (25-50 uL) of VECTASHIELD.

That depends upon how long you wish to retain the slides and which VECTASHIELD formulation you are using. If you are using one of our non-setting VECTASHIELD products such as H-1000/H-1200 or H-1900/H-2000, then we suggest sealing the coverslip with plastic sealant or nail polish if you intend to keep the slides beyond a day or so. If you are using one of our setting/curing formulations such as VECTASHIELD HardSet or Vibrance, then in most cases when using thin cut (<10 um) tissue sections or cell monolayers, no sealing of the coverslip is requuired.

Citations

Technical Information

VECTASHIELD Mounting Media are compatible with a wide array of fluorochromes, enzymatic substrates, and fluorescent proteins. Please consult the compatibility table (in the “Documents” section) to determine if VECTASHIELD will be compatible in your system.

All formulations of VECTASHIELD Antifade Mounting Media are available with or without the counterstain DAPI (4′, 6-diamidino-2-phenylindole).  The DAPI concentration can be modified by mixing with the corresponding VECTASHIELD Mounting Medium without DAPI.  DAPI produces a blue fluorescence when bound to DNA with excitation at about 360 nm and emission at 460 nm.

R.J. Florijn, et. al. Cytometry, 19 (1995) 177-182.

The refractive index for VECTASHIELD Mounting Medium is 1.45.

VECTASHIELD Mounting Medium in Super Resolution Microscopy

The optimal medium for super-resolution imaging methods maximizes the lifetime and photoswitching characteristics of fluorophores. VECTASHIELD Mounting Medium has been shown to be compatible with a number of fluorophores used in super resolution methods including STORM, STED, and 3D-SIM imaging, such as Cy5 or Alexa Fluor 647, resulting in greater convenience and reproducibility of the method. (Olivier, N. et al., 2013, Biomedical Optics Express, Vol. 4 No. 6, pp. 885-899; Glushonkov, O. et al., 2018, Scientific Reports, V 8, 8749 (2018) ).

VECTASHIELD Mounting Medium Antifade Comparison

Other manufacturers measure the antifade properties of their mountants using labeled microspheres or arrayed spots. Vector Labs prefers to measure antifade properties of VECTASHIELD mountants using frozen tissue sections immunohistochemically stained with fluorescently labeled secondary antibodies.  Antifade capability is measured using a 40x objective with real time imaging over 30 seconds of continuous exposure to the excitation illumination. Individual intensity measurements are recorded from 6 separate labeled regions and the average is calculated. The intensity after 30 second exposure is expressed as a percentage of the intensity at zero time. The values for PG are taken from the manufacturer s published results.

VSPGcomp jpg

VS.Chart .v2 jpg

Check out the below video for coverslipping tips for immunofluorescence slide mounting

02 15 23 banner jpg

How to mount a coverslip on a slide

Correctly coverslipping your slides ensures that your tissue specimens are preserved for optimal imaging and viewing. In this blog post, we will walk you through how to properly coverslip your slides to protect your samples.

august 10 banner 1

How to choose antifade mounting media

As far as antifade mounting media go, one-size-fits-all solutions are few and far between. Here, we break down key factors you should consider when choosing the best antifade mounting medium for your experiment.

Screenshot 2023 03 16 at 3.24.36 PM
Step by step protocol
Screenshot 2023 03 15 at 12.45.07 PM
Screenshot 2023 03 15 at 12.45.07 PM

The post VECTASHIELD® HardSet™ Antifade Mounting Medium with Phalloidin appeared first on VectorLabs.

]]> VECTASHIELD® HardSet™ Antifade Mounting Medium with DAPI https://staging.vectorlabs.com/products/vectashield-hardset-with-dapi/ Wed, 26 Apr 2023 03:59:54 +0000 https://staging.vectorlabs.com/?post_type=product&p=12142 Description

VECTASHIELD HardSet Antifade Mounting Medium with DAPI is a unique, stable formula for preserving fluorescence. This medium prevents rapid photobleaching of fluorescent proteins and fluorescent dyes. VECTASHIELD HardSet Mounting Medium with DAPI hardens after coverslipping. After approximately 15 minutes at room temperature, the coverslip will become immobilized, and optimal antifade ability and refractive index will be achieved.

Features:

  • Inhibits photobleaching of fluorescent dyes and fluorescent proteins
  • Ideal refractive index (1.46)
  • Ready-to-use – from the refrigerator to the benchtop
  • No warming necessary
  • Can be stored without sealing for long term analysis
  • Hardening formulation
  • DAPI counterstain

Specifications

Unit Size 10 ml
Applications Immunofluorescence, In situ hybridization, Cellular Imaging
Mounting Aqueous (Hardening)
Antifade Yes
Counterstain DAPI (blue fluorescence)
Cellular Stain Nucleus

The post VECTASHIELD® HardSet™ Antifade Mounting Medium with DAPI appeared first on VectorLabs.

]]>

Still using VectaShield® HardSet™ with DAPI?

Try our upgraded product VECTASHIELD® Vibrance™ Antifade Mounting Medium with DAPI

Description

VECTASHIELD HardSet Antifade Mounting Medium with DAPI is a unique, stable formula for preserving fluorescence. This medium prevents rapid photobleaching of fluorescent proteins and fluorescent dyes. VECTASHIELD HardSet Mounting Medium with DAPI hardens after coverslipping. After approximately 15 minutes at room temperature, the coverslip will become immobilized, and optimal antifade ability and refractive index will be achieved. 

Features:

  • Inhibits photobleaching of fluorescent dyes and fluorescent proteins
  • Ideal refractive index (1.46)
  • Ready-to-use – from the refrigerator to the benchtop
  • No warming necessary
  • Can be stored without sealing for long term analysis
  • Hardening formulation
  • DAPI counterstain

Specifications

Unit Size10 ml
ApplicationsImmunofluorescence, In situ hybridization, Cellular Imaging
MountingAqueous (Hardening)
AntifadeYes
CounterstainDAPI (blue fluorescence)
Cellular StainNucleus

Documents

Product FAQs

Fixed, thin cut (<10 um), glass mounted sections are recommended to be used in combination with this medium. Unfixed material, thicker sections or the use of gasket or chamber slides or well formats may increase the incidence of bubble formation when applying H-1500. If using thin sections as described, ensure an adequate volume of the medium is being applied to spread out under the coverslip. Storage of slides in the freezer (-20 °C) may also reduce the incidence of bubbles. For existing mounted sections with bubble formation, coverslips can be removed by soaking the slides in buffer, and then remounted using fresh media.

All of the VECTASHIELD formats we currently offer which includes regular non-setting VECTASHIELD, PLUS, HardSet and Vibrance formulations each contain a percentage of glycerol.

No form of tissue dehydration (e.g. air drying or ethanol exposure) is required nor recommended when applying VECTASHIELD. From our experience, the most optimal antifade actions of VECTASHIELD are obtained when the preparation is removed from the final buffer/water rinse, kept slightly wet/moist and then coverslipped with a small volume (25-50 uL) of VECTASHIELD.

That depends upon how long you wish to retain the slides and which VECTASHIELD formulation you are using. If you are using one of our non-setting VECTASHIELD products such as H-1000/H-1200 or H-1900/H-2000, then we suggest sealing the coverslip with plastic sealant or nail polish if you intend to keep the slides beyond a day or so. If you are using one of our setting/curing formulations such as VECTASHIELD HardSet or Vibrance, then in most cases when using thin cut (<10 um) tissue sections or cell monolayers.

Citations

Technical Information

VECTASHIELD Mounting Media are compatible with a wide array of fluorochromes, enzymatic substrates, and fluorescent proteins. Please consult the compatibility table (in the “Documents” section) to determine if VECTASHIELD will be compatible in your system.

All formulations of VECTASHIELD Antifade Mounting Media are available with or without the counterstain DAPI (4′, 6-diamidino-2-phenylindole).  The DAPI concentration can be modified by mixing with the corresponding VECTASHIELD Mounting Medium without DAPI.  DAPI produces a blue fluorescence when bound to DNA with excitation at about 360 nm and emission at 460 nm.

R.J. Florijn, et. al. Cytometry, 19 (1995) 177-182.

The refractive index for VECTASHIELD Mounting Medium is 1.45.

VECTASHIELD Mounting Medium in Super Resolution Microscopy

The optimal medium for super-resolution imaging methods maximizes the lifetime and photoswitching characteristics of fluorophores. VECTASHIELD Mounting Medium has been shown to be compatible with a number of fluorophores used in super resolution methods including STORM, STED, and 3D-SIM imaging, such as Cy5 or Alexa Fluor 647, resulting in greater convenience and reproducibility of the method. (Olivier, N. et al., 2013, Biomedical Optics Express, Vol. 4 No. 6, pp. 885-899; Glushonkov, O. et al., 2018, Scientific Reports, V 8, 8749 (2018) ).

VECTASHIELD Mounting Medium Antifade Comparison

Other manufacturers measure the antifade properties of their mountants using labeled microspheres or arrayed spots. Vector Labs prefers to measure antifade properties of VECTASHIELD mountants using frozen tissue sections immunohistochemically stained with fluorescently labeled secondary antibodies.  Antifade capability is measured using a 40x objective with real time imaging over 30 seconds of continuous exposure to the excitation illumination. Individual intensity measurements are recorded from 6 separate labeled regions and the average is calculated. The intensity after 30 second exposure is expressed as a percentage of the intensity at zero time. The values for PG are taken from the manufacturer s published results.

VSPGcomp jpg
VS.Chart .v2 jpg

Check out the video below on how to use a hydrophobic barrier pen.

Resources

02 15 23 banner jpg

How to mount a coverslip on a slide

Correctly coverslipping your slides ensures that your tissue specimens are preserved for optimal imaging and viewing. In this blog post, we will walk you through how to properly coverslip your slides to protect your samples.

august 10 banner 1

How to choose antifade mounting media

As far as antifade mounting media go, one-size-fits-all solutions are few and far between. Here, we break down key factors you should consider when choosing the best antifade mounting medium for your experiment.

Screenshot 2023 03 16 at 3.11.41 PM
Step by Step Protocol
Screenshot 2023 03 15 at 12.45.07 PM
Screenshot 2023 03 15 at 12.45.07 PM

The post VECTASHIELD® HardSet™ Antifade Mounting Medium with DAPI appeared first on VectorLabs.

]]> VECTASHIELD® HardSet™ Antifade Mounting Medium https://staging.vectorlabs.com/products/vectashield-hardset-antifade/ Wed, 26 Apr 2023 03:31:56 +0000 https://staging.vectorlabs.com/?post_type=product&p=12107 Description

VECTASHIELD HardSet Antifade Mounting Medium is a unique, stable formula for preserving fluorescence. This medium prevents rapid photobleaching of fluorescent proteins and fluorescent dyes. VECTASHIELD HardSet Mounting Medium hardens after coverslipping. After approximately 15 minutes at room temperature, the coverslip will become immobilized, and optimal antifade ability and refractive index will be achieved.

Features:

  • Inhibits photobleaching of dyes and fluorescent proteins
  • Ideal refractive index
  • Ready to use
  • No warming necessary
  • Offered with nuclear or cytoskeletal counterstains
  • Available in hardening formulation
  • Can be stored without sealing for long term analysis
  • Easy to use

Specifications

Unit Size 10 ml
Applications Immunofluorescence, In situ hybridization, Cellular Imaging
Mounting Aqueous (Hardening)
Antifade Yes
Counterstain None

The post VECTASHIELD® HardSet™ Antifade Mounting Medium appeared first on VectorLabs.

]]>

Still using VECTASHIELD HardSet?

Try our upgraded product VECTASHIELD® Vibrance™ Antifade Mounting Medium

Description

VECTASHIELD HardSet Antifade Mounting Medium is a unique, stable formula for preserving fluorescence. This medium prevents rapid photobleaching of fluorescent proteins and fluorescent dyes. VECTASHIELD HardSet Mounting Medium hardens after coverslipping. After approximately 15 minutes at room temperature, the coverslip will become immobilized, and optimal antifade ability and refractive index will be achieved.

Features:

  • Inhibits photobleaching of dyes and fluorescent proteins
  • Ideal refractive index
  • Ready to use
  • No warming necessary
  • Offered with nuclear or cytoskeletal counterstains
  • Available in hardening formulation
  • Can be stored without sealing for long term analysis
  • Easy to use

Specifications

Unit Size10 ml
ApplicationsImmunofluorescence, In situ hybridization, Cellular Imaging
MountingAqueous (Hardening)
AntifadeYes
CounterstainNone

Documents

Product FAQs

Fixed, thin cut (<10 um), glass mounted sections are recommended to be used in combination with this medium. Unfixed material, thicker sections or the use of gasket or chamber slides or well formats may increase the incidence of bubble formation when applying H-1500. If using thin sections as described, ensure an adequate volume of the medium is being applied to spread out under the coverslip. Storage of slides in the freezer (-20 °C) may also reduce the incidence of bubbles. For existing mounted sections with bubble formation, coverslips can be removed by soaking the slides in buffer, and then remounted using fresh media.

All of the VECTASHIELD formats we currently offer which includes regular non-setting VECTASHIELD, PLUS, HardSet and Vibrance formulations each contain a percentage of glycerol.

No form of tissue dehydration (e.g. air drying or ethanol exposure) is required nor recommended when applying VECTASHIELD. From our experience, the most optimal antifade actions of VECTASHIELD are obtained when the preparation is removed from the final buffer/water rinse, kept slightly wet/moist and then coverslipped with a small volume (25-50 uL) of VECTASHIELD.

That depends upon how long you wish to retain the slides and which VECTASHIELD formulation you are using. If you are using one of our non-setting VECTASHIELD products such as H-1000/H-1200 or H-1900/H-2000, then we suggest sealing the coverslip with plastic sealant or nail polish if you intend to keep the slides beyond a day or so. If you are using one of our setting/curing formulations such as VECTASHIELD HardSet or Vibrance, then in most cases when using thin cut (<10 um) tissue sections or cell monolayers.

Citations

Technical Information

VECTASHIELD Mounting Media are compatible with a wide array of fluorochromes, enzymatic substrates, and fluorescent proteins. Please consult the compatibility table (in the “Documents” section) to determine if VECTASHIELD will be compatible in your system.

All formulations of VECTASHIELD Antifade Mounting Media are available with or without the counterstain DAPI (4′, 6-diamidino-2-phenylindole).  The DAPI concentration can be modified by mixing with the corresponding VECTASHIELD Mounting Medium without DAPI.  DAPI produces a blue fluorescence when bound to DNA with excitation at about 360 nm and emission at 460 nm.

R.J. Florijn, et. al. Cytometry, 19 (1995) 177-182.

The refractive index for VECTASHIELD Mounting Medium is 1.45.

VECTASHIELD Mounting Medium in Super Resolution Microscopy

The optimal medium for super-resolution imaging methods maximizes the lifetime and photoswitching characteristics of fluorophores. VECTASHIELD Mounting Medium has been shown to be compatible with a number of fluorophores used in super resolution methods including STORM, STED, and 3D-SIM imaging, such as Cy5 or Alexa Fluor 647, resulting in greater convenience and reproducibility of the method. (Olivier, N. et al., 2013, Biomedical Optics Express, Vol. 4 No. 6, pp. 885-899; Glushonkov, O. et al., 2018, Scientific Reports, V 8, 8749 (2018) ).

VECTASHIELD Mounting Medium Antifade Comparison

Other manufacturers measure the antifade properties of their mountants using labeled microspheres or arrayed spots. Vector Labs prefers to measure antifade properties of VECTASHIELD mountants using frozen tissue sections immunohistochemically stained with fluorescently labeled secondary antibodies.  Antifade capability is measured using a 40x objective with real time imaging over 30 seconds of continuous exposure to the excitation illumination. Individual intensity measurements are recorded from 6 separate labeled regions and the average is calculated. The intensity after 30 second exposure is expressed as a percentage of the intensity at zero time. The values for PG are taken from the manufacturer s published results.

VSPGcomp jpg
VS.Chart .v2 jpg

Check out the video below on how to use a hydrophobic barrier pen.

Resources

02 15 23 banner jpg

How to mount a coverslip on a slide

Correctly coverslipping your slides ensures that your tissue specimens are preserved for optimal imaging and viewing. In this blog post, we will walk you through how to properly coverslip your slides to protect your samples.

august 10 banner 1

How to choose antifade mounting media

As far as antifade mounting media go, one-size-fits-all solutions are few and far between. Here, we break down key factors you should consider when choosing the best antifade mounting medium for your experiment.

Screenshot 2023 03 15 at 12.45.07
Step by Step Protocol
Screenshot 2023 03 15 at 12.45.07 PM
Screenshot 2023 03 15 at 12.45.07 PM

The post VECTASHIELD® HardSet™ Antifade Mounting Medium appeared first on VectorLabs.

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